LOCUS       DBBBSB010000001        84798 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.001, whole genome shotgun sequence.
ACCESSION   DBBBSB010000001 DBBBSB010000000
VERSION     DBBBSB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84798
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.001"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACIUS0_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACIUS0_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGB0217942.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACIUS0_000002"
     CDS             complement(1672..2667)
                     /locus_tag="ACIUS0_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGB0217943.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACIUS0_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACIUS0_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACIUS0_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACIUS0_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGB0217944.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACIUS0_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACIUS0_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGB0217945.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACIUS0_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACIUS0_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGB0217946.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACIUS0_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACIUS0_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGB0217947.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACIUS0_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACIUS0_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGB0217948.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACIUS0_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACIUS0_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGB0217949.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACIUS0_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACIUS0_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGB0217950.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACIUS0_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACIUS0_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGB0217951.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACIUS0_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACIUS0_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGB0217952.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACIUS0_000013"
     CDS             complement(17699..18322)
                     /locus_tag="ACIUS0_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGB0217953.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACIUS0_000014"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACIUS0_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGB0217954.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACIUS0_000015"
     CDS             complement(18971..19213)
                     /locus_tag="ACIUS0_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGB0217955.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACIUS0_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACIUS0_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGB0217956.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="ACIUS0_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="ACIUS0_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGB0217957.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACIUS0_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACIUS0_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGB0217958.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="ACIUS0_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="ACIUS0_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="ACIUS0_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="ACIUS0_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGB0217959.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="ACIUS0_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="ACIUS0_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGB0217960.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACIUS0_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="ACIUS0_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGB0217961.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..29673)
                     /locus_tag="ACIUS0_000024"
     CDS             complement(29257..29673)
                     /locus_tag="ACIUS0_000024"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGB0217962.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(29670..29777)
                     /locus_tag="ACIUS0_000025"
     CDS             complement(29670..29777)
                     /locus_tag="ACIUS0_000025"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0217963.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACIUS0_000026"
                     /pseudo
     CDS             complement(29738..30340)
                     /gene="ypdF"
                     /locus_tag="ACIUS0_000026"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="ACIUS0_000027"
     CDS             complement(30355..31602)
                     /locus_tag="ACIUS0_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGB0217964.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="ACIUS0_000028"
     CDS             complement(31624..31950)
                     /locus_tag="ACIUS0_000028"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGB0217965.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACIUS0_000029"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="ACIUS0_000029"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGB0217966.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="ACIUS0_000030"
     CDS             33338..34594
                     /locus_tag="ACIUS0_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGB0217967.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /gene="ypeC"
                     /locus_tag="ACIUS0_000031"
     CDS             34709..35035
                     /gene="ypeC"
                     /locus_tag="ACIUS0_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGB0217968.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="ACIUS0_000032"
     CDS             complement(35176..36414)
                     /locus_tag="ACIUS0_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGB0217969.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="ACIUS0_000033"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="ACIUS0_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGB0217970.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="ACIUS0_000034"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="ACIUS0_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(40322..40397)
                     /locus_tag="ACIUS0_000035"
     tRNA            complement(40322..40397)
                     /locus_tag="ACIUS0_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="ACIUS0_000036"
     tRNA            complement(40437..40512)
                     /locus_tag="ACIUS0_000036"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="ACIUS0_000037"
     CDS             40733..41092
                     /locus_tag="ACIUS0_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGB0217971.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="ACIUS0_000038"
     CDS             41094..41486
                     /locus_tag="ACIUS0_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGB0217972.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACIUS0_000039"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="ACIUS0_000039"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGB0217973.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="ACIUS0_000040"
     tRNA            43212..43287
                     /locus_tag="ACIUS0_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="ACIUS0_000041"
     tRNA            43332..43407
                     /locus_tag="ACIUS0_000041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="ACIUS0_000042"
     tRNA            43412..43487
                     /locus_tag="ACIUS0_000042"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="ACIUS0_000043"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="ACIUS0_000043"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="ACIUS0_000044"
     CDS             43763..44104
                     /locus_tag="ACIUS0_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGB0217974.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="ACIUS0_000045"
     CDS             complement(44095..45021)
                     /locus_tag="ACIUS0_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0217975.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="ACIUS0_000046"
     CDS             45111..46109
                     /locus_tag="ACIUS0_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGB0217976.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="ACIUS0_000047"
     CDS             complement(46106..46324)
                     /locus_tag="ACIUS0_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGB0217977.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACIUS0_000048"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="ACIUS0_000048"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGB0217978.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACIUS0_000049"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="ACIUS0_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGB0217979.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="ACIUS0_000050"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="ACIUS0_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGB0217980.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="ACIUS0_000051"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="ACIUS0_000051"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGB0217981.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="ACIUS0_000052"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="ACIUS0_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGB0217982.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="ACIUS0_000053"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="ACIUS0_000053"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGB0217983.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADS
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="ACIUS0_000054"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="ACIUS0_000054"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGB0217984.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACIUS0_000055"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="ACIUS0_000055"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGB0217985.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="ACIUS0_000056"
     CDS             55506..55880
                     /locus_tag="ACIUS0_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGB0217986.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="ACIUS0_000057"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="ACIUS0_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACIUS0_000058"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="ACIUS0_000058"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGB0217987.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACIUS0_000059"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="ACIUS0_000059"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGB0217988.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACIUS0_000060"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="ACIUS0_000060"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGB0217989.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACIUS0_000061"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="ACIUS0_000061"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGB0217990.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACIUS0_000062"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="ACIUS0_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGB0217991.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACIUS0_000063"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="ACIUS0_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGB0217992.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACIUS0_000064"
                     /pseudo
     CDS             complement(62920..63778)
                     /gene="murR"
                     /locus_tag="ACIUS0_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="ACIUS0_000065"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="ACIUS0_000065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGB0217993.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="ACIUS0_000066"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="ACIUS0_000066"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGB0217994.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            66271..66357
                     /locus_tag="ACIUS0_000067"
                     /pseudo
     CDS             66271..66357
                     /locus_tag="ACIUS0_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACIUS0_000068"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="ACIUS0_000068"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGB0217995.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="ACIUS0_000069"
     CDS             complement(67441..68016)
                     /locus_tag="ACIUS0_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGB0217996.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="ACIUS0_000070"
     CDS             complement(68077..68526)
                     /locus_tag="ACIUS0_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGB0217997.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="ACIUS0_000071"
     CDS             complement(68513..68938)
                     /locus_tag="ACIUS0_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGB0217998.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="ACIUS0_000072"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="ACIUS0_000072"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGB0217999.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="ACIUS0_000073"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="ACIUS0_000073"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGB0218000.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACIUS0_000074"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="ACIUS0_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACIUS0_000075"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="ACIUS0_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGB0218001.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACIUS0_000076"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="ACIUS0_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGB0218002.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73210..73278)
                     /locus_tag="ACIUS0_000077"
                     /pseudo
     CDS             complement(73210..73278)
                     /locus_tag="ACIUS0_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74382..75173
                     /locus_tag="ACIUS0_000078"
     CDS             74382..75173
                     /locus_tag="ACIUS0_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218003.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="ACIUS0_000079"
     CDS             75893..76288
                     /locus_tag="ACIUS0_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGB0218004.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="ACIUS0_000080"
     CDS             76306..76569
                     /locus_tag="ACIUS0_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218005.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGGSISAFK"
     gene            76592..77134
                     /locus_tag="ACIUS0_000081"
     CDS             76592..77134
                     /locus_tag="ACIUS0_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218006.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="ACIUS0_000082"
     CDS             77273..77560
                     /locus_tag="ACIUS0_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218007.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="ACIUS0_000083"
     CDS             77599..77805
                     /locus_tag="ACIUS0_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218008.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78117..78747
                     /locus_tag="ACIUS0_000084"
                     /pseudo
     CDS             78117..78747
                     /locus_tag="ACIUS0_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            78854..80314
                     /gene="gadC"
                     /locus_tag="ACIUS0_000085"
     CDS             78854..80314
                     /gene="gadC"
                     /locus_tag="ACIUS0_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HGB0218009.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80348..81157
                     /gene="murI"
                     /locus_tag="ACIUS0_000086"
     CDS             80348..81157
                     /gene="murI"
                     /locus_tag="ACIUS0_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGB0218010.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81276..81970)
                     /locus_tag="ACIUS0_000087"
     CDS             complement(join(81276..81724,81724..81970))
                     /locus_tag="ACIUS0_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0218011.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="ACIUS0_000088"
     CDS             complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="ACIUS0_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGB0218012.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="ACIUS0_000089"
     CDS             complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="ACIUS0_000089"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGB0218013.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="ACIUS0_000090"
     CDS             complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="ACIUS0_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGB0218014.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT        22033 a        21460 c        20102 g        21203 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacatgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gactcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg gtgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggt cctatttacc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattactg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgggtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    78121 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    78181 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    78241 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    78301 tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    78361 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    78421 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    78481 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    78541 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    78601 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    78661 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    78721 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    78781 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatgtt
    78841 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    78901 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    78961 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    79021 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    79081 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    79141 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    79201 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    79261 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    79321 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    79381 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    79441 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    79501 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    79561 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    79621 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    79681 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    79741 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    79801 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    79861 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    79921 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    79981 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    80041 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    80101 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    80161 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    80221 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    80281 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    80341 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg tcggtggatt
    80401 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    80461 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    80521 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    80581 atcctcaact ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat
    80641 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    80701 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    80761 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    80821 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    80881 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    80941 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    81001 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    81061 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    81121 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtac accgtaaaat
    81181 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    81241 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    81301 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    81361 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    81421 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    81481 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    81541 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    81601 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    81661 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    81721 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    81781 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    81841 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    81901 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    81961 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    82021 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    82081 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    82141 aaaagcagaa cattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac
    82201 taacaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    82261 tttttatttt tcattttctc ttttcttctc tttccggttc tgttctggtt ttggtttttt
    82321 cctttttatt tttattcttt cttcagggtt atgctaaaac accaaaacat ttttcaccac
    82381 aaaagcatcc aatcatcttg ccagaccaat caccttaacc ggatacactg ccggatataa
    82441 tcccactaac acccagacac caagcaccat acctgataac taccgtattc aggaaggaaa
    82501 tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg taccaatgtc
    82561 gatatagtcc cccgcgcgga caatcacttc gtcaatgact gccaacggga gttgttgtag
    82621 ctgtgggcgc aacaacatgc ccagagcttt accaaagtcc tgcccggcca ccaccagcag
    82681 gggatgcgga ttcggaaaac gcgcgacgaa atcgaccagc gcgttgatga ccgtcagaac
    82741 cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc acgtacacgt cagttttggg
    82801 atcaagatcc agctgaagca gcgcctgttg ccaggcactc accagatccg tttcatcaat
    82861 cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg ccctccagcc agattgtgct
    82921 gccagagagc gaaagggtat gtgcacccgc gccaatcacc gtggagcgta cggtttgcgc
    82981 cggaaactgc acattcatct cacgcaggcg cggatggtca tgcagcgccg ttgccagaag
    83041 tgggccaata tcggcaaaac agaacgggtt ggcgggctgg tggcgataac attcgcccac
    83101 gcctccagaa agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat
    83161 caatgcctgc gcgagcggcg aaagcgttcc gtcaatcact tcgacaatca gcgccgccat
    83221 ccgccgggta acctgcacca gctgcgcgcc ggtcagcgaa cgggcgtcag tgcctgcacc
    83281 gaagcactca tccacaatca tctgccccgg tttatgagcg taaaccacgc gcccctggct
    83341 gtcggtttcc agcaggcgac caccgacgtt gagacaggca gtgccgctga tttttccggc
    83401 atcgaacagg gcgtagttcg cggtgccacc gccgatatcg atattcagta cccgacacag
    83461 ccgttgttca gaaagggttt gcgccccggc tccgtgaccg gcgatcacgg attcgaggtg
    83521 cggcccggcg ctggcgacga caaaatcgcc cagcgactgt ttttccttca tcactttttc
    83581 catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt
    83641 ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca cggacgccgt
    83701 taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg
    83761 ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc
    83821 gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg
    83881 tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct
    83941 cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag
    84001 catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca
    84061 gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag atcagcatgt
    84121 ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac
    84181 ccggcgtatc aatatcgccc ttatcattaa attccacggc cagtgttttt ctggcgaggg
    84241 tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg ctacccacaa
    84301 acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat
    84361 ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg
    84421 agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg
    84481 ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc
    84541 caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg ggatgcgcga
    84601 tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat
    84661 ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa
    84721 taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat caataagaat ggagagatat
    84781 ttactgttat gaataaac
//
LOCUS       DBBBSB010000002        71465 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.002, whole genome shotgun sequence.
ACCESSION   DBBBSB010000002 DBBBSB010000000
VERSION     DBBBSB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.002"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(740..2326)
                     /locus_tag="ACIUS0_000091"
     CDS             complement(740..2326)
                     /locus_tag="ACIUS0_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HGB0218015.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2681..4852)
                     /gene="glcB"
                     /locus_tag="ACIUS0_000092"
     CDS             complement(2681..4852)
                     /gene="glcB"
                     /locus_tag="ACIUS0_000092"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGB0218016.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4874..5278)
                     /locus_tag="ACIUS0_000093"
     CDS             complement(4874..5278)
                     /locus_tag="ACIUS0_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGB0218017.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5283..6502)
                     /gene="glcF"
                     /locus_tag="ACIUS0_000094"
                     /pseudo
     CDS             complement(5283..6502)
                     /gene="glcF"
                     /locus_tag="ACIUS0_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6513..7565)
                     /gene="glcE"
                     /locus_tag="ACIUS0_000095"
     CDS             complement(6513..7565)
                     /gene="glcE"
                     /locus_tag="ACIUS0_000095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGB0218018.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7565..9064)
                     /gene="glcD"
                     /locus_tag="ACIUS0_000096"
                     /pseudo
     CDS             complement(7565..9064)
                     /gene="glcD"
                     /locus_tag="ACIUS0_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9315..10085
                     /gene="glcC"
                     /locus_tag="ACIUS0_000097"
     CDS             9315..10085
                     /gene="glcC"
                     /locus_tag="ACIUS0_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGB0218019.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10209..11350)
                     /gene="yghO"
                     /locus_tag="ACIUS0_000098"
                     /pseudo
     CDS             complement(10209..11350)
                     /gene="yghO"
                     /locus_tag="ACIUS0_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            11754..13442
                     /locus_tag="ACIUS0_000099"
     CDS             11754..13442
                     /locus_tag="ACIUS0_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGB0218020.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            13439..14353
                     /locus_tag="ACIUS0_000100"
     CDS             13439..14353
                     /locus_tag="ACIUS0_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGB0218021.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14386..14634
                     /locus_tag="ACIUS0_000101"
     CDS             14386..14634
                     /locus_tag="ACIUS0_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGB0218022.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14634..15805
                     /locus_tag="ACIUS0_000102"
                     /pseudo
     CDS             14634..15805
                     /locus_tag="ACIUS0_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15841..16920)
                     /gene="lptG"
                     /locus_tag="ACIUS0_000103"
     CDS             complement(15841..16920)
                     /gene="lptG"
                     /locus_tag="ACIUS0_000103"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGB0218023.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16917..17987)
                     /gene="lptF"
                     /locus_tag="ACIUS0_000104"
     CDS             complement(16917..17987)
                     /gene="lptF"
                     /locus_tag="ACIUS0_000104"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGB0218024.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18018..18578)
                     /locus_tag="ACIUS0_000105"
     CDS             complement(18018..18578)
                     /locus_tag="ACIUS0_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGB0218025.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18590..19414)
                     /locus_tag="ACIUS0_000106"
     CDS             complement(18590..19414)
                     /locus_tag="ACIUS0_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218026.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19414..20763)
                     /locus_tag="ACIUS0_000107"
     CDS             complement(19414..20763)
                     /locus_tag="ACIUS0_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGB0218027.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20809..21567)
                     /locus_tag="ACIUS0_000108"
     CDS             complement(20809..21567)
                     /locus_tag="ACIUS0_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218028.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21599..22312)
                     /locus_tag="ACIUS0_000109"
     CDS             complement(21599..22312)
                     /locus_tag="ACIUS0_000109"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGB0218029.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22486..23178
                     /locus_tag="ACIUS0_000110"
     CDS             22486..23178
                     /locus_tag="ACIUS0_000110"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGB0218030.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23227..24726)
                     /gene="pitB"
                     /locus_tag="ACIUS0_000111"
     CDS             complement(23227..24726)
                     /gene="pitB"
                     /locus_tag="ACIUS0_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGB0218031.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25018..26877)
                     /gene="gss"
                     /locus_tag="ACIUS0_000112"
     CDS             complement(25018..26877)
                     /gene="gss"
                     /locus_tag="ACIUS0_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGB0218032.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27082..27948
                     /gene="yghU"
                     /locus_tag="ACIUS0_000113"
     CDS             27082..27948
                     /gene="yghU"
                     /locus_tag="ACIUS0_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGB0218033.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(27977..28058)
                     /locus_tag="ACIUS0_000114"
                     /pseudo
     CDS             complement(27977..28058)
                     /locus_tag="ACIUS0_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28071..28319)
                     /gene="hybG"
                     /locus_tag="ACIUS0_000115"
     CDS             complement(28071..28319)
                     /gene="hybG"
                     /locus_tag="ACIUS0_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGB0218034.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28453..28794)
                     /gene="hypA"
                     /locus_tag="ACIUS0_000116"
     CDS             complement(28453..28794)
                     /gene="hypA"
                     /locus_tag="ACIUS0_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGB0218035.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28787..29275)
                     /gene="hybE"
                     /locus_tag="ACIUS0_000117"
     CDS             complement(28787..29275)
                     /gene="hybE"
                     /locus_tag="ACIUS0_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGB0218036.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29268..29762)
                     /locus_tag="ACIUS0_000118"
     CDS             complement(29268..29762)
                     /locus_tag="ACIUS0_000118"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGB0218037.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29762..31465)
                     /gene="hybC"
                     /locus_tag="ACIUS0_000119"
     CDS             complement(29762..31465)
                     /gene="hybC"
                     /locus_tag="ACIUS0_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGB0218038.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31462..32640)
                     /gene="hybB"
                     /locus_tag="ACIUS0_000120"
     CDS             complement(31462..32640)
                     /gene="hybB"
                     /locus_tag="ACIUS0_000120"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGB0218039.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32630..33616)
                     /gene="hybA"
                     /locus_tag="ACIUS0_000121"
     CDS             complement(32630..33616)
                     /gene="hybA"
                     /locus_tag="ACIUS0_000121"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGB0218040.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33619..34737)
                     /gene="hybO"
                     /locus_tag="ACIUS0_000122"
     CDS             complement(33619..34737)
                     /gene="hybO"
                     /locus_tag="ACIUS0_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGB0218041.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34926..35213)
                     /gene="yghW"
                     /locus_tag="ACIUS0_000123"
     CDS             complement(34926..35213)
                     /gene="yghW"
                     /locus_tag="ACIUS0_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGB0218042.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35332..36219)
                     /gene="yghX"
                     /locus_tag="ACIUS0_000124"
     CDS             complement(35332..36219)
                     /gene="yghX"
                     /locus_tag="ACIUS0_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGB0218043.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36376..37416
                     /gene="gpr"
                     /locus_tag="ACIUS0_000125"
     CDS             36376..37416
                     /gene="gpr"
                     /locus_tag="ACIUS0_000125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGB0218044.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37456..37950)
                     /locus_tag="ACIUS0_000126"
     CDS             complement(37456..37950)
                     /locus_tag="ACIUS0_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGB0218045.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38141..39025
                     /gene="yghA"
                     /locus_tag="ACIUS0_000127"
     CDS             38141..39025
                     /gene="yghA"
                     /locus_tag="ACIUS0_000127"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGB0218046.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39297..39722)
                     /gene="exbD"
                     /locus_tag="ACIUS0_000128"
     CDS             complement(39297..39722)
                     /gene="exbD"
                     /locus_tag="ACIUS0_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGB0218047.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39729..40463)
                     /gene="exbB"
                     /locus_tag="ACIUS0_000129"
     CDS             complement(39729..40463)
                     /gene="exbB"
                     /locus_tag="ACIUS0_000129"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGB0218048.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40715..41902
                     /gene="metC"
                     /locus_tag="ACIUS0_000130"
     CDS             40715..41902
                     /gene="metC"
                     /locus_tag="ACIUS0_000130"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGB0218049.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42042..42701
                     /gene="yghB"
                     /locus_tag="ACIUS0_000131"
     CDS             42042..42701
                     /gene="yghB"
                     /locus_tag="ACIUS0_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGB0218050.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42741..43640)
                     /gene="yqhC"
                     /locus_tag="ACIUS0_000132"
     CDS             complement(42741..43640)
                     /gene="yqhC"
                     /locus_tag="ACIUS0_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGB0218051.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43834..44997
                     /gene="yqhD"
                     /locus_tag="ACIUS0_000133"
     CDS             43834..44997
                     /gene="yqhD"
                     /locus_tag="ACIUS0_000133"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGB0218052.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45102..45929
                     /gene="dkgA"
                     /locus_tag="ACIUS0_000134"
     CDS             45102..45929
                     /gene="dkgA"
                     /locus_tag="ACIUS0_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGB0218053.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46129..47055
                     /locus_tag="ACIUS0_000135"
     CDS             46129..47055
                     /locus_tag="ACIUS0_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGB0218054.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47106..47363
                     /gene="yqhH"
                     /locus_tag="ACIUS0_000136"
     CDS             47106..47363
                     /gene="yqhH"
                     /locus_tag="ACIUS0_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGB0218055.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47406..49625)
                     /locus_tag="ACIUS0_000137"
                     /pseudo
     CDS             complement(47406..49625)
                     /locus_tag="ACIUS0_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(49736..51148)
                     /gene="ftsP"
                     /locus_tag="ACIUS0_000138"
     CDS             complement(49736..51148)
                     /gene="ftsP"
                     /locus_tag="ACIUS0_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGB0218056.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51223..51960)
                     /gene="plsC"
                     /locus_tag="ACIUS0_000139"
                     /gene_synonym="parF"
     CDS             complement(51223..51960)
                     /gene="plsC"
                     /locus_tag="ACIUS0_000139"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGB0218057.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52194..54452)
                     /gene="parC"
                     /locus_tag="ACIUS0_000140"
     CDS             complement(52194..54452)
                     /gene="parC"
                     /locus_tag="ACIUS0_000140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGB0218058.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(54998..55480)
                     /locus_tag="ACIUS0_000141"
     CDS             complement(54998..55480)
                     /locus_tag="ACIUS0_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGB0218059.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55533..55925)
                     /gene="ygiW"
                     /locus_tag="ACIUS0_000142"
     CDS             complement(55533..55925)
                     /gene="ygiW"
                     /locus_tag="ACIUS0_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGB0218060.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56077..56736
                     /gene="qseB"
                     /locus_tag="ACIUS0_000143"
     CDS             56077..56736
                     /gene="qseB"
                     /locus_tag="ACIUS0_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGB0218061.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56733..58007
                     /gene="qseC"
                     /locus_tag="ACIUS0_000144"
     CDS             56733..58007
                     /gene="qseC"
                     /locus_tag="ACIUS0_000144"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGB0218062.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58053..58385)
                     /locus_tag="ACIUS0_000145"
     CDS             complement(58053..58385)
                     /locus_tag="ACIUS0_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGB0218063.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58704..59285
                     /gene="mdaB"
                     /locus_tag="ACIUS0_000146"
     CDS             58704..59285
                     /gene="mdaB"
                     /locus_tag="ACIUS0_000146"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGB0218064.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59316..59630
                     /locus_tag="ACIUS0_000147"
     CDS             59316..59630
                     /locus_tag="ACIUS0_000147"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGB0218065.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59678..61570)
                     /gene="parE"
                     /locus_tag="ACIUS0_000148"
     CDS             complement(59678..61570)
                     /gene="parE"
                     /locus_tag="ACIUS0_000148"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGB0218066.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61599..62180)
                     /gene="yqiA"
                     /locus_tag="ACIUS0_000149"
     CDS             complement(61599..62180)
                     /gene="yqiA"
                     /locus_tag="ACIUS0_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGB0218067.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62180..63007)
                     /gene="cpdA"
                     /locus_tag="ACIUS0_000150"
     CDS             complement(62180..63007)
                     /gene="cpdA"
                     /locus_tag="ACIUS0_000150"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGB0218068.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63032..63454)
                     /locus_tag="ACIUS0_000151"
     CDS             complement(63032..63454)
                     /locus_tag="ACIUS0_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGB0218069.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63455..64084)
                     /gene="nudF"
                     /locus_tag="ACIUS0_000152"
     CDS             complement(63455..64084)
                     /gene="nudF"
                     /locus_tag="ACIUS0_000152"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGB0218070.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64289..65770
                     /gene="tolC"
                     /locus_tag="ACIUS0_000153"
     CDS             64289..65770
                     /gene="tolC"
                     /locus_tag="ACIUS0_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGB0218071.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65918..66589
                     /locus_tag="ACIUS0_000154"
     CDS             65918..66589
                     /locus_tag="ACIUS0_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGB0218072.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66595..67755
                     /locus_tag="ACIUS0_000155"
     CDS             66595..67755
                     /locus_tag="ACIUS0_000155"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGB0218073.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67793..68581)
                     /gene="ygiD"
                     /locus_tag="ACIUS0_000156"
     CDS             complement(67793..68581)
                     /gene="ygiD"
                     /locus_tag="ACIUS0_000156"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGB0218074.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68724..69497
                     /gene="zupT"
                     /locus_tag="ACIUS0_000157"
     CDS             68724..69497
                     /gene="zupT"
                     /locus_tag="ACIUS0_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGB0218075.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69555..69725)
                     /gene="yqiD"
                     /locus_tag="ACIUS0_000158"
     CDS             complement(69555..69725)
                     /gene="yqiD"
                     /locus_tag="ACIUS0_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGB0218076.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69986..70639)
                     /gene="ribB"
                     /locus_tag="ACIUS0_000159"
     CDS             complement(69986..70639)
                     /gene="ribB"
                     /locus_tag="ACIUS0_000159"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGB0218077.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70743..70891)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71013..71303
                     /gene="ubiK"
                     /locus_tag="ACIUS0_000160"
     CDS             71013..71303
                     /gene="ubiK"
                     /locus_tag="ACIUS0_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGB0218078.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17830 a        19051 c        17654 g        16930 t
ORIGIN      
        1 acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca caaacaggca
       61 ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca taatcaccaa
      121 attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg ttggagttaa
      181 aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg gatgatttcg
      241 aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta acgaacactg
      301 ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt ggagataaat
      361 tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa aatcagcttt
      421 gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt gttctagtga
      481 aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga agcaatgtaa
      541 taacatacta aaattgttct catgaaagtg ggtactttat aaccagcaca gaagccaagt
      601 tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag aagccaatcg
      661 taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt gatatttacc
      721 ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac gcctgcagca
      781 gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac agttcagatt
      841 ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg gagatcatct
      901 tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag acgttgattt
      961 gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg tccgacccgg
     1021 taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc acgcctgtac
     1081 ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg aaggcgaatg
     1141 ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt tcggcaaaga
     1201 cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc gccgcaataa
     1261 aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc attggcgttg
     1321 gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg atctggaaat
     1381 taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac ggcttcatgg
     1441 tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga atgatttgcc
     1501 ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc accatcgcac
     1561 ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag actttaagga
     1621 ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt tccggaccaa
     1681 tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc agcgccgctg
     1741 gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag aacggcacaa
     1801 gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac ggatcgattc
     1861 ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc accgtcgcac
     1921 caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt aacacctgca
     1981 aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac tgcccgctgg
     2041 caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt ggccataatc
     2101 cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt ttaaaggcga
     2161 atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt cctttcagac
     2221 gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc agagcggata
     2281 gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg ttcagtctct
     2341 tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg actgaactgt
     2401 aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg ctacctctgg
     2461 cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag agtatcgtta
     2521 agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt taatattgtt
     2581 aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag ttactatcat
     2641 agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt tctttttcgc
     2701 gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc acgccgagga
     2761 agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc gccatcggac
     2821 gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc tccagcgacg
     2881 cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata tgctggctgg
     2941 agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc ggcacttttg
     3001 aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc tgcacgttgt
     3061 tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc accggaatag
     3121 tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg gcaatgttgg
     3181 cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta gcagcggttg
     3241 gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc tggctgtaca
     3301 tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct ttaccgcgca
     3361 gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc cacggcgtcg
     3421 atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc atttcatcgc
     3481 cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc tgagcgatac
     3541 agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt ttcagggtat
     3601 tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg aacgccactt
     3661 cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc gagtttttct
     3721 gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga ataccttccg
     3781 ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga cccacgttgc
     3841 ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg gcggtgtact
     3901 gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt ttctcttgca
     3961 gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct tccgcatcaa
     4021 ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg acgataacat
     4081 cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt tgcagttcaa
     4141 tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg ccacggtaac
     4201 cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac tggatgcgta
     4261 attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt tccagcggta
     4321 gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc tgcggatcgt
     4381 agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac gcatcgtaca
     4441 gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt gccggaacca
     4501 ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt tccaccgtca
     4561 cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta taggccgctt
     4621 tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc tgaatgcgat
     4681 cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc tcatcaacat
     4741 tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca tccacaaaac
     4801 gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc attgtttctc
     4861 tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc tgcgcatcct
     4921 gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca acaacaaccg
     4981 gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta cgtccgttgt
     5041 tcaccatctc ttcatagccc ttagtttcac gacgccccag cgcggcggta cgcgctttct
     5101 cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac gccagcagat
     5161 gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc gcctcttcct
     5221 gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta gttttcattt
     5281 ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac agaggtacga
     5341 ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat ttccggtttg
     5401 ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc gggatgcgtt
     5461 aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc cgttaaggta
     5521 aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc atgttgtagg
     5581 gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt ttccagcggt
     5641 tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg cgctttatcg
     5701 gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc gccgcaaccg
     5761 ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca ggcatcaata
     5821 ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc caccgcgcca
     5881 caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag cactcgcgca
     5941 gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag cattaaaacc
     6001 cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc aggcagtttt
     6061 gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt cagcgcacgg
     6121 aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg ctccggcagt
     6181 gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt gtgatagcgc
     6241 acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc gagatgctcc
     6301 tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata gatgcgcccg
     6361 cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc ggtacaaaat
     6421 ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc tgccgcatct
     6481 cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat gcgaccgggg
     6541 ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg gaataaagga
     6601 gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg accgccagcg
     6661 ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca gcgtaacgcc
     6721 ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc gctgggtaat
     6781 gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc acgcaattgc
     6841 tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc ttttaccgat
     6901 ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa gccactaatg
     6961 ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag gctgatttcc
     7021 cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat ttcagtgagc
     7081 acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc ggcaacgttt
     7141 ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat gcgcgtgccg
     7201 aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag cccgcaggcg
     7261 accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg aagcatttgc
     7321 ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg agcggttatc
     7381 accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc cagcgtttgc
     7441 ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac cagcggcgtt
     7501 ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc acactcgcgt
     7561 agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca tgcatggcac
     7621 caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc aaaccatcgg
     7681 ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg aactgggcgc
     7741 acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg ccgccaactt
     7801 caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat tcaccaggtt
     7861 cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga aagacgttgg
     7921 caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg ccaggcaggg
     7981 cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt cctaccgccg
     8041 ggaacgcatt tttgcgaccg gcccagaaac gtacgcgctc tgcttcgtcc tgtgccaaac
     8101 ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag tcttcctgta
     8161 cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg acgggataac
     8221 cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc tccagcccgc
     8281 cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt tctaccgagt
     8341 caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt accgtcactt
     8401 cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg tcaaaaccag
     8461 gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt tgcacttcaa
     8521 ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca ccggcatttt
     8581 ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc gcgtaataga
     8641 gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc tgcacgcgcg
     8701 cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc accaacaaca
     8761 cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca cgggtcacca
     8821 ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc atttgcttag
     8881 gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac tcgtaaggaa
     8941 tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc agtgccatca
     9001 gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg tacaagatgc
     9061 tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc gctgcttctg
     9121 tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc ggtcagacca
     9181 ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg atgtaacata
     9241 atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt cctacctgtg
     9301 cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt gcagagagta
     9361 tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc tcggaacgtc
     9421 gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg accgtgctgc
     9481 gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca cggcttaatc
     9541 gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga acgctgtacg
     9601 atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg gcaacgctgg
     9661 gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc gccgccagtg
     9721 agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct ttccatctcg
     9781 ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca ttgaccgatc
     9841 tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca cagcaaaaaa
     9901 agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg ctgccgcacg
     9961 tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg
    10021 agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac aaggtgggaa
    10081 tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac ttaacaggat
    10141 ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc gctaaacagg
    10201 atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc cgccccaatg
    10261 cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat ctccagcgca
    10321 tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc
    10381 gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt
    10441 cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg aaagagcgat
    10501 ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac gcgcagggtg
    10561 cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta agttggtcgc
    10621 ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc tgccagccag
    10681 agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga ttgatgcagc
    10741 gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga gagaaagtga
    10801 gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa cccagttgtt
    10861 caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt ggtgtgtcaa
    10921 aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc ccgctgatct
    10981 tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg gcaaaatcct
    11041 gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg taacgctcac
    11101 ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct ttttttgcca
    11161 cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa tgctcgttgc
    11221 gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat gacggaaaag
    11281 cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc atcagcagat
    11341 cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt tgcaacagca
    11401 acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat agtattaatt
    11461 aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt gtcaatttaa
    11521 ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt cataataaaa
    11581 acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat gaaaatacta
    11641 tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta tttttgatat
    11701 ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta cctatgcgct
    11761 atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt agagccggaa
    11821 tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag acattaaaaa
    11881 cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa ggcgaacgcg
    11941 tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc tgtcagtatg
    12001 ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg gattcctgga
    12061 gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc actggtgatg
    12121 agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat gttttaagcc
    12181 acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca gttccgaacg
    12241 atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc gtcattatca
    12301 cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt aaattacgcc
    12361 ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg gtcggctttc
    12421 tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag gattttgcca
    12481 tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt tccgttgcgc
    12541 cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc gctgaactgg
    12601 atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca gaacaactcc
    12661 atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc atgccgtgct
    12721 acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc ggggttgtgg
    12781 ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg ccgggtgcag
    12841 agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa catggtattg
    12901 aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat atttgcatct
    12961 ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac gagattgccg
    13021 cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat ctgtatgtca
    13081 ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg caggatattg
    13141 aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct tttgttaccg
    13201 cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa cgtcgcgggc
    13261 agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc gcggctatcg
    13321 atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc cgtgcggaag
    13381 cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa tccctggcat
    13441 aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata ttatcgataa
    13501 cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg ctcacgtcaa
    13561 tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta gcgagctggt
    13621 tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca aagaagagat
    13681 tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa aagagagcgg
    13741 cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg agctctcctg
    13801 gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg acgaaattac
    13861 gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt taaaaccgct
    13921 gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata cacaactctc
    13981 ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg ccgaaactgt
    14041 acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact ggcaacgcgt
    14101 acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca ttcctctgcc
    14161 ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag gtaaagaacc
    14221 tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg caagtaataa
    14281 ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg cattacgcaa
    14341 cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt aaatcgtgaa
    14401 atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg tgtggaaatt
    14461 aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt gatggatctc
    14521 ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct gctggatgtc
    14581 aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa ataatggggc
    14641 tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac aacgggttaa
    14701 caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg atcggcaata
    14761 aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat catgatgcca
    14821 tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt tctcgtatgg
    14881 caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag tttttcaatc
    14941 gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact
    15001 tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgt
    15061 ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga
    15121 acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat
    15181 ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct
    15241 cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg caacggggcg
    15301 cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag
    15361 taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg aagtgctgcg
    15421 ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt
    15481 gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa
    15541 cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag
    15601 cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct
    15661 tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac
    15721 gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca
    15781 ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt
    15841 ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg
    15901 taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat
    15961 ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat
    16021 cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt
    16081 caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc tgggttgccc
    16141 ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg
    16201 cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa
    16261 tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca
    16321 cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc
    16381 gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac
    16441 cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc
    16501 ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac
    16561 ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag
    16621 tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga
    16681 taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag
    16741 tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg
    16801 gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc
    16861 tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat
    16921 cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc
    16981 atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt
    17041 gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac
    17101 gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc
    17161 aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag
    17221 gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc
    17281 agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga
    17341 tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg
    17401 tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt agaagtataa
    17461 atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt
    17521 ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc
    17581 gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga
    17641 agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc
    17701 ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc
    17761 gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact
    17821 ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc
    17881 agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc
    17941 accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc
    18001 tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct
    18061 acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc
    18121 accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg
    18181 tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca
    18241 aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga
    18301 tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc
    18361 gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc
    18421 cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata
    18481 taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag
    18541 agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa
    18601 aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat
    18661 aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca
    18721 ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa
    18781 gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa
    18841 attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt
    18901 gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca
    18961 ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa
    19021 tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc
    19081 cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct
    19141 tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt
    19201 tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt
    19261 gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg
    19321 gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct
    19381 cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata
    19441 aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc
    19501 cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa
    19561 catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg
    19621 ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat
    19681 tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag
    19741 cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac cgattccgcc
    19801 cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg
    19861 catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa
    19921 gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga tccccaccaa
    19981 cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac
    20041 aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt
    20101 atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac
    20161 cagattcgac acgtaccagg caataacaaa acccacaaaa ccaaagtcaa aataccaggc
    20221 tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc
    20281 aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag
    20341 cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag
    20401 gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga
    20461 gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt
    20521 tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc
    20581 gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca
    20641 cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct
    20701 attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc
    20761 caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact
    20821 tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc
    20881 gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga
    20941 catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc
    21001 agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg
    21061 gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac
    21121 gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct
    21181 gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg
    21241 cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct
    21301 ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa ccaacggcaa
    21361 tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg
    21421 ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc
    21481 acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt
    21541 aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc
    21601 aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc
    21661 gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt
    21721 tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa
    21781 tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact
    21841 cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc
    21901 catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc
    21961 cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat
    22021 agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt acatgtgccg
    22081 ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt
    22141 cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca
    22201 gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg
    22261 caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc
    22321 cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt
    22381 attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa aacatattaa
    22441 ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca
    22501 cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt gtgtgaacat
    22561 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
    22621 ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca
    22681 attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc ggcgctggta
    22741 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
    22801 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
    22861 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
    22921 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
    22981 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
    23041 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
    23101 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
    23161 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
    23221 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
    23281 cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
    23341 gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg
    23401 catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc
    23461 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
    23521 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
    23581 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
    23641 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc acagcataat
    23701 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
    23761 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
    23821 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
    23881 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
    23941 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
    24001 caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc cgtgcgaaaa
    24061 cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
    24121 gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
    24181 actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
    24241 ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac
    24301 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
    24361 gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
    24421 cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca gcaacaaatc
    24481 gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
    24541 aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat
    24601 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
    24661 aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt
    24721 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
    24781 acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt
    24841 atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact
    24901 ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat tgaatatttt
    24961 gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca acatgtatta
    25021 ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc
    25081 accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta
    25141 tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa
    25201 tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc
    25261 acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt
    25321 gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat
    25381 cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag
    25441 caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc
    25501 aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt
    25561 ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc
    25621 cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc
    25681 cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg
    25741 acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt
    25801 gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt
    25861 atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct catccatgca
    25921 gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg
    25981 tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt
    26041 gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta gctcctgctc
    26101 ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc
    26161 ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt caaactggcc
    26221 tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt ccggctgcgg
    26281 taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa
    26341 agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc gcgtccactg
    26401 ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc
    26461 atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca
    26521 gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg
    26581 caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat
    26641 acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac
    26701 gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc
    26761 ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg
    26821 ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac
    26881 tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct
    26941 cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc
    27001 aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta
    27061 ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg
    27121 tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg
    27181 catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct gggaacgccg
    27241 aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt tactggtgca
    27301 gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc
    27361 gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc
    27421 cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg
    27481 ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt acagggtgcc
    27541 gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag
    27601 tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa
    27661 ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg
    27721 ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg
    27781 ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa
    27841 cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat
    27901 gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg gttggtgttc
    27961 gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca
    28021 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca tcacgcgctg
    28081 gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg
    28141 atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg
    28201 ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc
    28261 gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt
    28321 gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc
    28381 cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc tttttaggcg
    28441 ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac
    28501 acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac
    28561 ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc
    28621 ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac
    28681 gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc
    28741 cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc
    28801 ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattggtga
    28861 ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg
    28921 acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa acaccgtcca
    28981 gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca
    29041 ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg
    29101 gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg
    29161 gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg
    29221 ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc
    29281 gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc
    29341 gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc
    29401 gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac
    29461 aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact
    29521 tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac aatggcatcc
    29581 gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg
    29641 ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc
    29701 cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc
    29761 attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac
    29821 aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat
    29881 cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac
    29941 gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt
    30001 taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga
    30061 actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat
    30121 tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg
    30181 tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag
    30241 tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg
    30301 ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag
    30361 tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg
    30421 tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt
    30481 cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca
    30541 gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg ctgtcggtcg
    30601 ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt
    30661 agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagtt
    30721 tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc aacccgtcga
    30781 ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc gttttaccgc
    30841 ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg
    30901 ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat
    30961 tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag
    31021 tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt
    31081 tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat
    31141 agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt
    31201 gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg
    31261 cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat
    31321 cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg
    31381 aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg
    31441 gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg
    31501 ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat
    31561 agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc
    31621 gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac ctaacagtgc
    31681 gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca cacgcagaac
    31741 gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc
    31801 ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa
    31861 aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg
    31921 accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc
    31981 catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct gccagagcgg
    32041 atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg
    32101 cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt gtagcgacac
    32161 cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta
    32221 gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat
    32281 gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag
    32341 tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg
    32401 gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat
    32461 cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga
    32521 gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc
    32581 aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat
    32641 gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc
    32701 ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg
    32761 aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac
    32821 ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat
    32881 atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt
    32941 attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg
    33001 taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat
    33061 cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt
    33121 tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg
    33181 tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaga
    33241 ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg
    33301 cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga
    33361 tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt
    33421 tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca
    33481 aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg
    33541 catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa
    33601 aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg
    33661 acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag
    33721 caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg
    33781 tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat
    33841 acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca caccgccaac
    33901 atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg
    33961 gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgaccagc
    34021 atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg
    34081 acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc
    34141 gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag
    34201 aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca
    34261 cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt tgcggatgtg
    34321 atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga
    34381 accatccacc actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc
    34441 ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc
    34501 cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt
    34561 gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc
    34621 catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt
    34681 catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta
    34741 tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa tcaataatgc
    34801 gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg
    34861 ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg
    34921 tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta
    34981 ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata
    35041 cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct gtgtgagtag
    35101 cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg
    35161 gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac
    35221 ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga cttttgcgca
    35281 cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct atcaggagag
    35341 atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata
    35401 acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat aagcctcata
    35461 aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt tgattcgggt
    35521 gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg
    35581 tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc
    35641 gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg tcgcttgcgg
    35701 atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac
    35761 ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa cggaacttaa
    35821 tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat
    35881 atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt tgccgctcat
    35941 ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt
    36001 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt
    36061 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc
    36121 aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa
    36181 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg
    36241 tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca
    36301 gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg
    36361 tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac
    36421 cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt atggcacaat
    36481 ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta
    36541 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
    36601 aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact gattatctct
    36661 accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc acgtaaatac
    36721 ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt cgatatcttt
    36781 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
    36841 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
    36901 caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg
    36961 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
    37021 ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
    37081 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg tggtctgacg
    37141 ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag
    37201 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
    37261 acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt gcaggcgctg
    37321 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
    37381 gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac
    37441 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
    37501 tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac
    37561 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
    37621 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca gcttctcttt
    37681 gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca tcaccatcac
    37741 cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga
    37801 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
    37861 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
    37921 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
    37981 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt
    38041 tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
    38101 tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc
    38161 gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
    38221 ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta gcggtcgcct
    38281 gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat
    38341 cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga
    38401 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg
    38461 cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg
    38521 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga
    38581 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct
    38641 aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca cttcgtcaat
    38701 ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct
    38761 gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt
    38821 cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat
    38881 cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
    38941 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt
    39001 gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
    39061 ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa
    39121 gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag
    39181 cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac gaagattcaa
    39241 catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat tctactttac
    39301 ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc
    39361 gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg
    39421 gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg
    39481 accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt
    39541 tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc
    39601 gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc
    39661 gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc
    39721 atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg
    39781 gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc
    39841 atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc
    39901 gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact
    39961 gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc
    40021 gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg
    40081 cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct
    40141 ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg
    40201 agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt
    40261 aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag
    40321 aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa aataagccca
    40381 atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac ggaaaggtcc
    40441 gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca
    40501 taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg catactgccg
    40561 tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc
    40621 gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt
    40681 agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca
    40741 actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca
    40801 gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg
    40861 cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca
    40921 agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc
    40981 ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac
    41041 cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac
    41101 atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa
    41161 aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat
    41221 tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc
    41281 agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac
    41341 caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg
    41401 ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc
    41461 ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag
    41521 cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc
    41581 tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg
    41641 gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga
    41701 taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc
    41761 aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac
    41821 cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc
    41881 cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga cattttattg
    41941 tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa
    42001 taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata
    42061 tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg
    42121 ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat
    42181 ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa ggggttatgg
    42241 attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt
    42301 atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc
    42361 ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc
    42421 tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg
    42481 gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg
    42541 tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc
    42601 aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca
    42661 cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg
    42721 ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc
    42781 cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag
    42841 ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc
    42901 ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa
    42961 ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg
    43021 ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact gaagtgagtc
    43081 tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga
    43141 atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg
    43201 agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt aatcccgctg
    43261 gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac
    43321 tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt
    43381 tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac
    43441 acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat gccaggctcg
    43501 tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac atcgggcaac
    43561 agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc cagcaagcga
    43621 caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca atttgattgt
    43681 gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat tggcagaaat
    43741 gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag atcgttctct
    43801 gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct gcacacccca
    43861 acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat tcctcacgat
    43921 gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt tctcgatcaa
    43981 gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga gccaaacccg
    44041 gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg
    44101 ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc agcggctaac
    44161 tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc
    44221 gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc caacgcaggc
    44281 gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc acatgttcag
    44341 ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg tcaggtggct
    44401 aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa accggttgat
    44461 gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga agatggtccg
    44521 aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg ggcggcgact
    44581 caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac gcatatgctg
    44641 ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc tatcgtcctg
    44701 cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca atatgctgaa
    44761 cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc gattgccgca
    44821 acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta cggtctggac
    44881 ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac ccaactgggc
    44941 gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc ccgctaagct
    45001 ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc acaacacctc
    45061 accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat ccaaccgtta
    45121 ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg caagcaagta
    45181 atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc tcgattgata
    45241 ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca
    45301 acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag cgcccccgcg
    45361 aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc tacttaatgc
    45421 actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg atcgaattgc
    45481 aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat cacctgcaac
    45541 gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt catccgctga
    45601 tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc gaatcctgga
    45661 gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc gatctggcag
    45721 ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat agcggcctgg
    45781 tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat gtctgggatt
    45841 tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc aagcgtctcg
    45901 gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc ggtaatgttc
    45961 cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt gttactcttc
    46021 cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc tcttaaaaga
    46081 acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat gaaaataata
    46141 cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc ttcacagacc
    46201 gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag tacgccttat
    46261 ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc gcttaccctg
    46321 aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc ggtttgtgat
    46381 tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac tgacgtcgat
    46441 catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa gaccacacag
    46501 acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt cagcaatcat
    46561 ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc tttagcttcg
    46621 ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat tgctgattat
    46681 agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa tgccttctat
    46741 aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga acggcaattt
    46801 atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt tgatgaaatt
    46861 cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac gctgaccaat
    46921 ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg ggaaatcgct
    46981 gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa aaccgccgcc
    47041 agattaaagc aatgagctga attaaataac aattagccgg aacaataaat aaaagggaac
    47101 actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc ttgctcagtg
    47161 gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca ttaaatgcca
    47221 aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat gctgccaaat
    47281 ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat tgcctgcgct
    47341 gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt tgccggggat
    47401 tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt caccggacga
    47461 gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt cgccatcggc
    47521 gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc acgcatctct
    47581 tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg ctttttgccc
    47641 atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta acgcaacaac
    47701 gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc gctcttataa
    47761 ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt cgccagcggc
    47821 gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag ccacagcgcc
    47881 aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata cgggatcaga
    47941 tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa gcggtcatag
    48001 ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg ttccggagca
    48061 atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg cggatcttcc
    48121 acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc tttcagatca
    48181 cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg cggacaaata
    48241 tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga tttgcagcgc
    48301 aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt aaaacctgga
    48361 acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg gctctgaata
    48421 atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca gccgcgcata
    48481 atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc ataggccgga
    48541 tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt agaaagcggg
    48601 atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa tgcgcgggcg
    48661 cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag cactttatcg
    48721 cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg tttctggcgc
    48781 ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg tttgttatcc
    48841 gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc tagggtatca
    48901 agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat aattgcggta
    48961 ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg cgctacttac
    49021 accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc taccagaacg
    49081 gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag gctagcctca
    49141 ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg ggtataaacc
    49201 agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc atcatcatga
    49261 cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc agcagtaaca
    49321 ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct ccagtccggc
    49381 tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca aatcgccatc
    49441 ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc
    49501 cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg tccaaaacag
    49561 gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag ggagatagag
    49621 ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta taagccggaa
    49681 cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat gaagattacg
    49741 gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc agcgtctgac
    49801 tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca agcagctcca
    49861 cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac ggcatcgcgc
    49921 cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc ggctcgtccg
    49981 cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata cggttgacgt
    50041 cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga ctgcgaattg
    50101 gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct gtgaccagcg
    50161 gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata ctggatggct
    50221 caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg atcgacactt
    50281 catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc agcgaaagtt
    50341 gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc
    50401 gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc agtcgcaagc
    50461 gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc agcagcgtat
    50521 caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag ttatccagcc
    50581 gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg ataggcagcg
    50641 acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag acctgctgag
    50701 ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg cgaataggca
    50761 gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc atcagcgggc
    50821 ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg ttgctgtaaa
    50881 taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa cgaccattga
    50941 ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt tgtacagtca
    51001 taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc ggttgctgtt
    51061 gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc cccgatgcct
    51121 gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc taaagtaata
    51181 ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt ttccggcggc
    51241 ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta tcgaacgaca
    51301 atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa ttggcggcag
    51361 catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt
    51421 agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa atgctccagt
    51481 cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca tccagatgga
    51541 aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag ctttagtgcg
    51601 attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga agaaggggat
    51661 ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt tcgatgctgt
    51721 caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt agctttcagc
    51781 atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac ggccaaacat
    51841 atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc
    51901 gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga tatatagcat
    51961 aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca ggttagggca
    52021 ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga ataaacaacg
    52081 gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg acgacttaac
    52141 gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc atattactct
    52201 tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc gatacgctgc
    52261 aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg tagctcttcc
    52321 ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg cggcggcaga
    52381 acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg aatgttgata
    52441 atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa catcaacata
    52501 cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac caccggcggc
    52561 ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt acgcgctacc
    52621 agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag cagtttctga
    52681 tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt taatttgccg
    52741 gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat ggcatagcta
    52801 cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac cgccgctttg
    52861 aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc tttagcgctg
    52921 cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag catgtcgtgc
    52981 tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag
    53041 gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg ctcggaagcc
    53101 aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc gcgaatcttc
    53161 atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt
    53221 tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt acgaatgatc
    53281 tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat atgcaggcgc
    53341 ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg acggaacacc
    53401 agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc cagaccgatc
    53461 atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag gtggttcatc
    53521 acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt cgggttctcg
    53581 tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg catttgcgca
    53641 gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct gataaccacc
    53701 gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc gttctcgtag
    53761 attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata atccggcccc
    53821 tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa tgcgattgcc
    53881 gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc gacggcaata
    53941 ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt cggctcctgc
    54001 aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc gctcaatagc
    54061 agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga
    54121 tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta agagaacggc
    54181 tgcgccatca ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg gtatttaccc
    54241 agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt caggcccagt
    54301 tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc aataaacggc
    54361 aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc cgtaaattca
    54421 tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac ttattcgcca
    54481 gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg agtcacagga
    54541 gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat caatgtgcct
    54601 tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa ggttgcgccc
    54661 atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg aggttatgcg
    54721 agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt cagcccagtt
    54781 tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg ctcatggata
    54841 tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg gtattcctta
    54901 ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc aaacttatcg
    54961 cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc acataaacat
    55021 ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga aacagaatcg
    55081 gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg ccccatatcg
    55141 tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt ccaccggtaa
    55201 gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg ctgccgcaga
    55261 tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg acgccaaaag
    55321 tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata aatttcgcca
    55381 cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca gggattgatg
    55441 ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc tgcgaaatta
    55501 ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg gatctgtttg
    55561 acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc aaccgtatct
    55621 ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt gatagtaccg
    55681 ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt gccgcgcagg
    55741 gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac gctgccgttc
    55801 ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa accgccctgc
    55861 tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc tgcgaatttt
    55921 ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat agtccttaac
    55981 aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac aacacggttt
    56041 actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact gatagaagat
    56101 gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt tagtgtcgac
    56161 tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta tgatgcggtg
    56221 atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga atggcgagag
    56281 aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc ggaacgtgta
    56341 gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt gatagaagtc
    56401 gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa cgagctgcgc
    56461 cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg cgaaccctta
    56521 acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc tggtcgggta
    56581 ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt caccagtaat
    56641 gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt tattcgtacc
    56701 gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt cttagtctgc
    56761 gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg ctttccagct
    56821 ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc caactgatgc
    56881 tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg gatcgcatgg
    56941 cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg acctttgcca
    57001 tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa gatattccct
    57061 atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac gatccttggc
    57121 gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc caggaatggg
    57181 aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg tggctggtcg
    57241 cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc gcgccgctga
    57301 acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca ctaaacgcga
    57361 ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg ttcgcccgca
    57421 cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac gaactacgta
    57481 gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac gatgatccgc
    57541 aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct actcgtctgg
    57601 ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag gacgtcgcgg
    57661 agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac acggcgcagc
    57721 aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc accggacaac
    57781 cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt gtgacaccag
    57841 aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc gctaccggca
    57901 gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg aatgttgagt
    57961 ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt ctctgtctat
    58021 gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa ccgtccccaa
    58081 aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag ccaaaactcg
    58141 aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt aagaagaaaa
    58201 aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg tatactgcaa
    58261 atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa agaatctatt
    58321 aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat tttctgcttt
    58381 aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac acgttgatat
    58441 tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg aaccaaacgt
    58501 gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt gtcattttca
    58561 gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct aaattcgcaa
    58621 aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta tagtcgacaa
    58681 acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc gcgaaaaaat
    58741 tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat ggcacactgc
    58801 gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat gtcaaagcgg
    58861 aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc tggtggatgg
    58921 gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt cacgggactc
    58981 tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt tccggcagcc
    59041 tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg gaagccttca
    59101 ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg ccgttccata
    59161 aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat gacgtgataa
    59221 aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg gaaatttttg
    59281 gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc gcagaaatcc
    59341 gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct aaaatcgttc
    59401 caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat tgcgctgctg
    59461 gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag tgggaaagca
    59521 tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc gaagccgtaa
    59581 aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa tcctgccttg
    59641 tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc tccgccatgt
    59701 cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc agcagcatat
    59761 ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc accagacggc
    59821 gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc cccagacctt
    59881 taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt acgccctctt
    59941 tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg tagagcggtg
    60001 gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt acgaacaaag
    60061 cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg atacagattt
    60121 tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc gctaccgaaa
    60181 tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag gtgttaagga
    60241 tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc ttggcagatc
    60301 cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc tgcgcggtac
    60361 aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc accacttttt
    60421 tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc aactccgccg
    60481 cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc gaaacgaatg
    60541 ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc ggatcctgca
    60601 ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac agcttcacgc
    60661 cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac aggccctgac
    60721 gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg tagctttcgg
    60781 tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct tcagtatcgc
    60841 cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt accgcttccg
    60901 ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg ttgatctcat
    60961 ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc acatgcgtca
    61021 ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag aagtgcacgc
    61081 tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc accttttcgc
    61141 cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact tctacgcgct
    61201 tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag aactggtaat
    61261 ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca accgccggta
    61321 ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc acttctaacg
    61381 actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt gcttcatcga
    61441 cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg gtatacatcc
    61501 ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca gcgttataag
    61561 tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg cagaccaaga
    61621 aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt gccgccttct
    61681 atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc cagcacttca
    61741 tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg
    61801 actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt gtaggggttc
    61861 tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg gtttaccacc
    61921 actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc cagtgacgaa
    61981 ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag cagttctgcc
    62041 gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg gtgatgttcc
    62101 gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt
    62161 aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt
    62221 ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg agagtacgcc
    62281 agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac tgcacacagg
    62341 tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc tgatgaatat
    62401 gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc agttcgcccg
    62461 cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga tgatgatgca
    62521 gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc cactcaagct
    62581 gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc aacaggattt
    62641 gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc tgtaacgcgc
    62701 tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc gcacgaaaac
    62761 ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc gctaaatcac
    62821 ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc agcaccgcct
    62881 ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca aacaggtgag
    62941 tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg gttaacaggc
    63001 tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc atcgctccat
    63061 gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc ttttcgtcgc
    63121 gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa atctgctggc
    63181 ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt gacggaaggc
    63241 tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg tatcgggtcg
    63301 attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca gtttcgccgg
    63361 gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg cacaggcgca
    63421 tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt tttaacgctt
    63481 gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct
    63541 cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata tcttcgtttt
    63601 catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc accataattg
    63661 acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt ttggtccgtt
    63721 tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc acactttcac
    63781 cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg gtgtcgtacg
    63841 cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca aagggtagca
    63901 agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc atttgcccgt
    63961 tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga taaagtgttt
    64021 ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg tctggcttaa
    64081 gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg acgtttgact
    64141 gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc acgtaacgcc
    64201 aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt tgatcgcgct
    64261 aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc ttatcggcct
    64321 gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag tttatcagca
    64381 agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga
    64441 aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg cagattacac
    64501 ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca gtgcgtccct
    64561 gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa
    64621 agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga tcctcaacac
    64681 cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata cacaggcaca
    64741 aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg tgggcctggt
    64801 agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt
    64861 gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca ccggtaacta
    64921 ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac cacagccggt
    64981 taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag
    65041 ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac cgactctgga
    65101 tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa cccgtggtgc
    65161 cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc tgagcttctc
    65221 gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac agtacaactt
    65281 tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa ccgtacgttc
    65341 ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac aagccgtagt
    65401 ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta cgcgtaccat
    65461 tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc tggcgaatgc
    65521 gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta cgttgaacga
    65581 gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta atccggaaaa
    65641 cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc ctgatagccc
    65701 ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc ataacccttt
    65761 ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca acgtaaagat
    65821 acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc catcctctat
    65881 tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa aatccatacg
    65941 ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg ctctcgcggt
    66001 tgccactgtt tttatgctgg ctggctgtga aaagagtgat gaaacagtgt ctctctatca
    66061 aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta
    66121 caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg
    66181 tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg gcatggcacc
    66241 agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc tgatggccgg
    66301 ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc cgctgttctc
    66361 ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg gtaaaaacta
    66421 tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg caccaaaacc
    66481 ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac
    66541 tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat accgatggaa
    66601 agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata cggtttcaat
    66661 tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa gttgaccctc
    66721 gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg cctgaaagtg
    66781 gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc aaaacacacc
    66841 tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc gcgtcttgat
    66901 ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga tacgccaacg
    66961 tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct taacgccggt
    67021 aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat cgatcgcttc
    67081 gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg tcgcgacacg
    67141 gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc tgaaatcgcc
    67201 accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt cacggattta
    67261 caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat gctgcgcgaa
    67321 atgttctcca ccaagctgga agatgctggc gtacgctggc tggaaccggc gtggaagagc
    67381 attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac
    67441 ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata tgtggttaaa
    67501 ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa
    67561 gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca cccgttaccg
    67621 aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca acctgccgga
    67681 attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt ttatccacat
    67741 atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat tgttagccta
    67801 tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga atggtaattg
    67861 gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc tctggcgttg
    67921 ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc tgctctaccg
    67981 gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc cacggatacg
    68041 gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta ccgctggcga
    68101 ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg aaatgccagg
    68161 cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg tcaggataca
    68221 tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta tccagcgtca
    68281 ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa cccggagcag
    68341 gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc gtgggcggcg
    68401 tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc accacaatcg
    68461 cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac aaattatctt
    68521 ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca
    68581 tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct tgttcggcgg
    68641 agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc tgcctgaagg
    68701 tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata ctggcggggg
    68761 cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg aaccgcttac
    68821 tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta atggaaatgc
    68881 ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga atgtttatct
    68941 tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat ccgcaggatt
    69001 taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc attctgctca
    69061 ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc acggcgagca
    69121 gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat atccctgaag
    69181 gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc gcgattctgt
    69241 gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg ttaatcctcg
    69301 gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca ggaattatgg
    69361 tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct aataataacc
    69421 ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa
    69481 cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca acttctgcca
    69541 gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact gtttaagtat
    69601 ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc
    69661 aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta ccaggcgata
    69721 aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa atttcacata
    69781 tcctttttcg gatatattca tatggtcgca taataacgga acaattatat gattttgatc
    69841 ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga agcggaatta
    69901 acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg gtttcttgat
    69961 taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc tgacggtatg
    70021 ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac tcaatgcact
    70081 ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca ccagccggtt
    70141 taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta
    70201 caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt
    70261 ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca gaaacaccgg
    70321 tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt
    70381 tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg atgcacaggc
    70441 aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc atggtttctg
    70501 ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc attacaccgc
    70561 gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta ccaaaagagg
    70621 aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg
    70681 gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat gcccgacaga
    70741 atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac cggatctgct
    70801 gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg
    70861 tggggaattt cgccccgccc tgagaataag cggattcact ataacgctaa tgattagcgg
    70921 cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc actacaatgg
    70981 tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa attgagcaaa
    71041 tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg gaagatgtgg
    71101 agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt gtaagccgtg
    71161 aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg gcgctgcttg
    71221 aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag ccagatccag
    71281 agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt atgaattaat
    71341 cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga gtatcatcac
    71401 aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt tttacttaat
    71461 cgata
//
LOCUS       DBBBSB010000003        70712 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.003, whole genome shotgun sequence.
ACCESSION   DBBBSB010000003 DBBBSB010000000
VERSION     DBBBSB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.003"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..795
                     /locus_tag="ACIUS0_000161"
     CDS             <1..795
                     /locus_tag="ACIUS0_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGB0218079.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACIUS0_000162"
     CDS             806..1897
                     /locus_tag="ACIUS0_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218080.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACIUS0_000163"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACIUS0_000163"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGB0218081.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACIUS0_000164"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACIUS0_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGB0218082.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACIUS0_000165"
     CDS             4859..5254
                     /locus_tag="ACIUS0_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGB0218083.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACIUS0_000166"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACIUS0_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGB0218084.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACIUS0_000167"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACIUS0_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGB0218085.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACIUS0_000168"
     CDS             complement(6563..7603)
                     /locus_tag="ACIUS0_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGB0218086.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACIUS0_000169"
     CDS             complement(7676..8311)
                     /locus_tag="ACIUS0_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGB0218087.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACIUS0_000170"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACIUS0_000170"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGB0218088.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACIUS0_000171"
     CDS             complement(8937..9380)
                     /locus_tag="ACIUS0_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGB0218089.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACIUS0_000172"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACIUS0_000172"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGB0218090.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACIUS0_000173"
     CDS             complement(9720..10223)
                     /locus_tag="ACIUS0_000173"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0218091.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACIUS0_000174"
     CDS             complement(10217..10741)
                     /locus_tag="ACIUS0_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGB0218092.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACIUS0_000175"
     CDS             10950..11945
                     /locus_tag="ACIUS0_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGB0218093.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACIUS0_000176"
     CDS             11954..12832
                     /locus_tag="ACIUS0_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGB0218094.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACIUS0_000177"
     CDS             12913..13920
                     /locus_tag="ACIUS0_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGB0218095.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACIUS0_000178"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACIUS0_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGB0218096.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACIUS0_000179"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACIUS0_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGB0218097.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACIUS0_000180"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACIUS0_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGB0218098.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACIUS0_000181"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACIUS0_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGB0218099.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACIUS0_000182"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACIUS0_000182"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGB0218100.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACIUS0_000183"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACIUS0_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGB0218101.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACIUS0_000184"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACIUS0_000184"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGB0218102.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACIUS0_000185"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACIUS0_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGB0218103.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACIUS0_000186"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACIUS0_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGB0218104.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACIUS0_000187"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACIUS0_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGB0218105.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACIUS0_000188"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACIUS0_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGB0218106.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACIUS0_000189"
     tRNA            complement(27654..27730)
                     /locus_tag="ACIUS0_000189"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACIUS0_000190"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACIUS0_000190"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGB0218107.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACIUS0_000191"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACIUS0_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACIUS0_000192"
     tRNA            complement(31514..31600)
                     /locus_tag="ACIUS0_000192"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACIUS0_000193"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACIUS0_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGB0218108.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACIUS0_000194"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACIUS0_000194"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGB0218109.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACIUS0_000195"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACIUS0_000195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGB0218110.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACIUS0_000196"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACIUS0_000196"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGB0218111.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACIUS0_000197"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACIUS0_000197"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGB0218112.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACIUS0_000198"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACIUS0_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGB0218113.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACIUS0_000199"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACIUS0_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGB0218114.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACIUS0_000200"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACIUS0_000200"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGB0218115.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACIUS0_000201"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACIUS0_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACIUS0_000202"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACIUS0_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGB0218116.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACIUS0_000203"
     CDS             complement(41212..42177)
                     /locus_tag="ACIUS0_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGB0218117.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACIUS0_000204"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACIUS0_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGB0218118.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACIUS0_000205"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACIUS0_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGB0218119.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACIUS0_000206"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACIUS0_000206"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGB0218120.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACIUS0_000207"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACIUS0_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGB0218121.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACIUS0_000208"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACIUS0_000208"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGB0218122.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACIUS0_000209"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACIUS0_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGB0218123.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACIUS0_000210"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACIUS0_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGB0218124.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACIUS0_000211"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACIUS0_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGB0218125.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACIUS0_000212"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACIUS0_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGB0218126.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACIUS0_000213"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACIUS0_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGB0218127.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACIUS0_000214"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACIUS0_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGB0218128.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACIUS0_000215"
     CDS             49715..50680
                     /locus_tag="ACIUS0_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGB0218129.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACIUS0_000216"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACIUS0_000216"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGB0218130.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACIUS0_000217"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACIUS0_000217"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGB0218131.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACIUS0_000218"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACIUS0_000218"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGB0218132.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACIUS0_000219"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACIUS0_000219"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGB0218133.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACIUS0_000220"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACIUS0_000220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGB0218134.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACIUS0_000221"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACIUS0_000221"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGB0218135.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACIUS0_000222"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACIUS0_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGB0218136.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACIUS0_000223"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACIUS0_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGB0218137.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACIUS0_000224"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACIUS0_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGB0218138.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACIUS0_000225"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACIUS0_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGB0218139.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACIUS0_000226"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACIUS0_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGB0218140.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACIUS0_000227"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACIUS0_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACIUS0_000228"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACIUS0_000228"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGB0218141.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACIUS0_000229"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACIUS0_000229"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGB0218142.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACIUS0_000230"
     CDS             complement(62547..63476)
                     /locus_tag="ACIUS0_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGB0218143.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACIUS0_000231"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACIUS0_000231"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGB0218144.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACIUS0_000232"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACIUS0_000232"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGB0218145.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACIUS0_000233"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACIUS0_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17314 a        19176 c        17560 g        16662 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DBBBSB010000004        67427 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.004, whole genome shotgun sequence.
ACCESSION   DBBBSB010000004 DBBBSB010000000
VERSION     DBBBSB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67427
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.004"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..229
                     /locus_tag="ACIUS0_000234"
     CDS             <1..229
                     /locus_tag="ACIUS0_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HGB0218146.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="ACIUS0_000235"
                     /pseudo
     CDS             624..1263
                     /locus_tag="ACIUS0_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1366..1689
                     /locus_tag="ACIUS0_000236"
     CDS             1366..1689
                     /locus_tag="ACIUS0_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HGB0218147.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="ACIUS0_000237"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="ACIUS0_000237"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGB0218148.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="ACIUS0_000238"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="ACIUS0_000238"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGB0218149.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="ACIUS0_000239"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="ACIUS0_000239"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGB0218150.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="ACIUS0_000240"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="ACIUS0_000240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGB0218151.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="ACIUS0_000241"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="ACIUS0_000241"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGB0218152.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="ACIUS0_000242"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="ACIUS0_000242"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGB0218153.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="ACIUS0_000243"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="ACIUS0_000243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGB0218154.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="ACIUS0_000244"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="ACIUS0_000244"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGB0218155.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="ACIUS0_000245"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="ACIUS0_000245"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGB0218156.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="ACIUS0_000246"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="ACIUS0_000246"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGB0218157.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="ACIUS0_000247"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="ACIUS0_000247"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGB0218158.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="ACIUS0_000248"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="ACIUS0_000248"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGB0218159.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="ACIUS0_000249"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="ACIUS0_000249"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGB0218160.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="ACIUS0_000250"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="ACIUS0_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGB0218161.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="ACIUS0_000251"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="ACIUS0_000251"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGB0218162.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="ACIUS0_000252"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="ACIUS0_000252"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGB0218163.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="ACIUS0_000253"
     CDS             complement(21227..23059)
                     /locus_tag="ACIUS0_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGB0218164.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="ACIUS0_000254"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="ACIUS0_000254"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGB0218165.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="ACIUS0_000255"
     CDS             complement(23807..24301)
                     /locus_tag="ACIUS0_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGB0218166.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /gene="yfbV"
                     /locus_tag="ACIUS0_000256"
     CDS             complement(24384..24839)
                     /gene="yfbV"
                     /locus_tag="ACIUS0_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGB0218167.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="ACIUS0_000257"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="ACIUS0_000257"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGB0218168.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="ACIUS0_000258"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="ACIUS0_000258"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGB0218169.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="ACIUS0_000259"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="ACIUS0_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGB0218170.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLVTWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="ACIUS0_000260"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="ACIUS0_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGB0218171.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="ACIUS0_000261"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="ACIUS0_000261"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGB0218172.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="ACIUS0_000262"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="ACIUS0_000262"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGB0218173.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="ACIUS0_000263"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="ACIUS0_000263"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGB0218174.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="ACIUS0_000264"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="ACIUS0_000264"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGB0218175.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="ACIUS0_000265"
     CDS             33415..34308
                     /locus_tag="ACIUS0_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGB0218176.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="ACIUS0_000266"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="ACIUS0_000266"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGB0218177.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="ACIUS0_000267"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="ACIUS0_000267"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGB0218178.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="ACIUS0_000268"
     CDS             complement(36224..36940)
                     /locus_tag="ACIUS0_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0218179.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="ACIUS0_000269"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="ACIUS0_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGB0218180.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="ACIUS0_000270"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="ACIUS0_000270"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGB0218181.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="ACIUS0_000271"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="ACIUS0_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGB0218182.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="ACIUS0_000272"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="ACIUS0_000272"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGB0218183.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="ACIUS0_000273"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="ACIUS0_000273"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGB0218184.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="ACIUS0_000274"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="ACIUS0_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGB0218185.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="ACIUS0_000275"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="ACIUS0_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGB0218186.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="ACIUS0_000276"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="ACIUS0_000276"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGB0218187.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="ACIUS0_000277"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="ACIUS0_000277"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGB0218188.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="ACIUS0_000278"
     CDS             complement(45789..46448)
                     /locus_tag="ACIUS0_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGB0218189.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="ACIUS0_000279"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="ACIUS0_000279"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGB0218190.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="ACIUS0_000280"
     CDS             complement(47343..48356)
                     /locus_tag="ACIUS0_000280"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGB0218191.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="ACIUS0_000281"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="ACIUS0_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGB0218192.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="ACIUS0_000282"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="ACIUS0_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGB0218193.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="ACIUS0_000283"
     CDS             complement(50649..51827)
                     /locus_tag="ACIUS0_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0218194.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="ACIUS0_000284"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="ACIUS0_000284"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGB0218195.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="ACIUS0_000285"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="ACIUS0_000285"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGB0218196.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="ACIUS0_000286"
     CDS             complement(55609..55887)
                     /locus_tag="ACIUS0_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGB0218197.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="ACIUS0_000287"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="ACIUS0_000287"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGB0218198.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="ACIUS0_000288"
     CDS             complement(56469..57278)
                     /locus_tag="ACIUS0_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HGB0218199.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="ACIUS0_000289"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="ACIUS0_000289"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGB0218200.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="ACIUS0_000290"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="ACIUS0_000290"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGB0218201.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="ACIUS0_000291"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="ACIUS0_000291"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGB0218202.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="ACIUS0_000292"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="ACIUS0_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGB0218203.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="ACIUS0_000293"
     CDS             complement(60979..61869)
                     /locus_tag="ACIUS0_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGB0218204.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="ACIUS0_000294"
     CDS             complement(61856..62380)
                     /locus_tag="ACIUS0_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218205.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="ACIUS0_000295"
     CDS             complement(62377..62847)
                     /locus_tag="ACIUS0_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218206.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="ACIUS0_000296"
     CDS             complement(62844..63392)
                     /locus_tag="ACIUS0_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218207.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="ACIUS0_000297"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="ACIUS0_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..66779)
                     /gene="yfcU"
                     /locus_tag="ACIUS0_000298"
                     /pseudo
     CDS             complement(64139..66779)
                     /gene="yfcU"
                     /locus_tag="ACIUS0_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66861..67424)
                     /locus_tag="ACIUS0_000299"
     CDS             complement(66861..67424)
                     /locus_tag="ACIUS0_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218208.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16833 a        18243 c        16744 g        15607 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaatga
      601 cgccttttat tgataattta catatgaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctcatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaagaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gtcacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag gctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttacggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattat
//
LOCUS       DBBBSB010000005        66984 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.005, whole genome shotgun sequence.
ACCESSION   DBBBSB010000005 DBBBSB010000000
VERSION     DBBBSB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66984
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.005"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="ACIUS0_000300"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="ACIUS0_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGB0218209.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="ACIUS0_000301"
     CDS             complement(1151..1237)
                     /locus_tag="ACIUS0_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGB0218210.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="ACIUS0_000302"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="ACIUS0_000302"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGB0218211.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="ACIUS0_000303"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="ACIUS0_000303"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGB0218212.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="ACIUS0_000304"
     CDS             3586..3630
                     /locus_tag="ACIUS0_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218213.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="ACIUS0_000305"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="ACIUS0_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGB0218214.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="ACIUS0_000306"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="ACIUS0_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGB0218215.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="ACIUS0_000307"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="ACIUS0_000307"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGB0218216.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="ACIUS0_000308"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="ACIUS0_000308"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGB0218217.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="ACIUS0_000309"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="ACIUS0_000309"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGB0218218.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="ACIUS0_000310"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="ACIUS0_000310"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGB0218219.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACIUS0_000311"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="ACIUS0_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGB0218220.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACIUS0_000312"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="ACIUS0_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGB0218221.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="ACIUS0_000313"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="ACIUS0_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGB0218222.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="ACIUS0_000314"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="ACIUS0_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGB0218223.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="ACIUS0_000315"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="ACIUS0_000315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGB0218224.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="ACIUS0_000316"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="ACIUS0_000316"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGB0218225.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="ACIUS0_000317"
     CDS             complement(16981..17745)
                     /locus_tag="ACIUS0_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGB0218226.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACIUS0_000318"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="ACIUS0_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGB0218227.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="ACIUS0_000319"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="ACIUS0_000319"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGB0218228.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="ACIUS0_000320"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="ACIUS0_000320"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGB0218229.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="ACIUS0_000321"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="ACIUS0_000321"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGB0218230.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="ACIUS0_000322"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="ACIUS0_000322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGB0218231.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="ACIUS0_000323"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="ACIUS0_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGB0218232.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="ACIUS0_000324"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="ACIUS0_000324"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGB0218233.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACIUS0_000325"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="ACIUS0_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGB0218234.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="ACIUS0_000326"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="ACIUS0_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGB0218235.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="ACIUS0_000327"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="ACIUS0_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGB0218236.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="ACIUS0_000328"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="ACIUS0_000328"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGB0218237.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="ACIUS0_000329"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="ACIUS0_000329"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGB0218238.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="ACIUS0_000330"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="ACIUS0_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGB0218239.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="ACIUS0_000331"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="ACIUS0_000331"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGB0218240.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLSWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACIUS0_000332"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="ACIUS0_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGB0218241.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACIUS0_000333"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="ACIUS0_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGB0218242.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACIUS0_000334"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="ACIUS0_000334"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGB0218243.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="ACIUS0_000335"
     CDS             complement(40421..41815)
                     /locus_tag="ACIUS0_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0218244.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACIUS0_000336"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="ACIUS0_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGB0218245.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="ACIUS0_000337"
     CDS             complement(42158..43444)
                     /locus_tag="ACIUS0_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGB0218246.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="ACIUS0_000338"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="ACIUS0_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="ACIUS0_000339"
     CDS             complement(44451..45221)
                     /locus_tag="ACIUS0_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGB0218247.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="ACIUS0_000340"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="ACIUS0_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGB0218248.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="ACIUS0_000341"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="ACIUS0_000341"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGB0218249.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="ACIUS0_000342"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="ACIUS0_000342"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGB0218250.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="ACIUS0_000343"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="ACIUS0_000343"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGB0218251.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="ACIUS0_000344"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="ACIUS0_000344"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGB0218252.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="ACIUS0_000345"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="ACIUS0_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGB0218253.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="ACIUS0_000346"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="ACIUS0_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGB0218254.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="ACIUS0_000347"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="ACIUS0_000347"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGB0218255.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="ACIUS0_000348"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="ACIUS0_000348"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGB0218256.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="ACIUS0_000349"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="ACIUS0_000349"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGB0218257.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="ACIUS0_000350"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="ACIUS0_000350"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGB0218258.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="ACIUS0_000351"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="ACIUS0_000351"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGB0218259.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="ACIUS0_000352"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="ACIUS0_000352"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGB0218260.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="ACIUS0_000353"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="ACIUS0_000353"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGB0218261.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="ACIUS0_000354"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="ACIUS0_000354"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGB0218262.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="ACIUS0_000355"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="ACIUS0_000355"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGB0218263.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="ACIUS0_000356"
     CDS             complement(66071..66289)
                     /locus_tag="ACIUS0_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGB0218264.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="ACIUS0_000357"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="ACIUS0_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGB0218265.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16247 a        18238 c        17245 g        15254 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgagctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcctcattag acct
//
LOCUS       DBBBSB010000006        60419 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.006, whole genome shotgun sequence.
ACCESSION   DBBBSB010000006 DBBBSB010000000
VERSION     DBBBSB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.006"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..322
                     /locus_tag="ACIUS0_000358"
     CDS             <1..322
                     /locus_tag="ACIUS0_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0218266.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACIUS0_000359"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACIUS0_000359"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGB0218267.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACIUS0_000360"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACIUS0_000360"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGB0218268.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACIUS0_000361"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACIUS0_000361"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGB0218269.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACIUS0_000362"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACIUS0_000362"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGB0218270.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACIUS0_000363"
     CDS             complement(3726..4955)
                     /locus_tag="ACIUS0_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGB0218271.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACIUS0_000364"
     CDS             complement(5019..5459)
                     /locus_tag="ACIUS0_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGB0218272.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACIUS0_000365"
     CDS             complement(5564..6334)
                     /locus_tag="ACIUS0_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0218273.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACIUS0_000366"
     CDS             complement(6331..7257)
                     /locus_tag="ACIUS0_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0218274.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACIUS0_000367"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACIUS0_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGB0218275.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACIUS0_000368"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACIUS0_000368"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGB0218276.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACIUS0_000369"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACIUS0_000369"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGB0218277.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACIUS0_000370"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACIUS0_000370"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGB0218278.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACIUS0_000371"
     CDS             12964..13311
                     /locus_tag="ACIUS0_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGB0218279.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACIUS0_000372"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACIUS0_000372"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGB0218280.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACIUS0_000373"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACIUS0_000373"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGB0218281.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACIUS0_000374"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACIUS0_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGB0218282.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACIUS0_000375"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACIUS0_000375"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGB0218283.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACIUS0_000376"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACIUS0_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACIUS0_000377"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACIUS0_000377"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGB0218284.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACIUS0_000378"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACIUS0_000378"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGB0218285.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACIUS0_000379"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACIUS0_000379"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGB0218286.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACIUS0_000380"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACIUS0_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGB0218287.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACIUS0_000381"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACIUS0_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGB0218288.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACIUS0_000382"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACIUS0_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGB0218289.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACIUS0_000383"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACIUS0_000383"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGB0218290.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACIUS0_000384"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACIUS0_000384"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGB0218291.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACIUS0_000385"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACIUS0_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGB0218292.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACIUS0_000386"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACIUS0_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACIUS0_000387"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACIUS0_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGB0218293.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDTMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACIUS0_000388"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACIUS0_000388"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGB0218294.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACIUS0_000389"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACIUS0_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACIUS0_000390"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACIUS0_000390"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGB0218295.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACIUS0_000391"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACIUS0_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGB0218296.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACIUS0_000392"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACIUS0_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGB0218297.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACIUS0_000393"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACIUS0_000393"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGB0218298.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACIUS0_000394"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACIUS0_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGB0218299.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACIUS0_000395"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACIUS0_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGB0218300.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACIUS0_000396"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACIUS0_000396"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGB0218301.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACIUS0_000397"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACIUS0_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGB0218302.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACIUS0_000398"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACIUS0_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGB0218303.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACIUS0_000399"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACIUS0_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGB0218304.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACIUS0_000400"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACIUS0_000400"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGB0218305.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACIUS0_000401"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACIUS0_000401"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGB0218306.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKLQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACIUS0_000402"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACIUS0_000402"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGB0218307.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACIUS0_000403"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACIUS0_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGB0218308.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACIUS0_000404"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACIUS0_000404"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGB0218309.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACIUS0_000405"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACIUS0_000405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGB0218310.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACIUS0_000406"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACIUS0_000406"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGB0218311.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACIUS0_000407"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACIUS0_000407"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGB0218312.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACIUS0_000408"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACIUS0_000408"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGB0218313.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACIUS0_000409"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACIUS0_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGB0218314.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACIUS0_000410"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACIUS0_000410"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGB0218315.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACIUS0_000411"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACIUS0_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGB0218316.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14474 a        16732 c        14847 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatacg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcagcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DBBBSB010000007        58574 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.007, whole genome shotgun sequence.
ACCESSION   DBBBSB010000007 DBBBSB010000000
VERSION     DBBBSB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58574
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.007"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1101)
                     /locus_tag="ACIUS0_000412"
     CDS             complement(<1..1101)
                     /locus_tag="ACIUS0_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGB0218317.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACIUS0_000413"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACIUS0_000413"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGB0218318.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACIUS0_000414"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACIUS0_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGB0218319.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACIUS0_000415"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACIUS0_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGB0218320.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACIUS0_000416"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACIUS0_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGB0218321.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACIUS0_000417"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACIUS0_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGB0218322.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACIUS0_000418"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACIUS0_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGB0218323.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACIUS0_000419"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACIUS0_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGB0218324.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACIUS0_000420"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACIUS0_000420"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGB0218325.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACIUS0_000421"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACIUS0_000421"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGB0218326.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACIUS0_000422"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACIUS0_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGB0218327.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACIUS0_000423"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACIUS0_000423"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGB0218328.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACIUS0_000424"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACIUS0_000424"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGB0218329.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACIUS0_000425"
     CDS             11851..12015
                     /locus_tag="ACIUS0_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218330.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACIUS0_000426"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACIUS0_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGB0218331.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACIUS0_000427"
     CDS             13524..13769
                     /locus_tag="ACIUS0_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGB0218332.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACIUS0_000428"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACIUS0_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGB0218333.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACIUS0_000429"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACIUS0_000429"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGB0218334.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACIUS0_000430"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACIUS0_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGB0218335.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACIUS0_000431"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACIUS0_000431"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGB0218336.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACIUS0_000432"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACIUS0_000432"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGB0218337.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACIUS0_000433"
     CDS             19432..19785
                     /locus_tag="ACIUS0_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGB0218338.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACIUS0_000434"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACIUS0_000434"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGB0218339.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACIUS0_000435"
     CDS             21085..22212
                     /locus_tag="ACIUS0_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGB0218340.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACIUS0_000436"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACIUS0_000436"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGB0218341.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="ACIUS0_000437"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="ACIUS0_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="ACIUS0_000438"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="ACIUS0_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACIUS0_000439"
     CDS             complement(26405..26533)
                     /gene="ymiD"
                     /locus_tag="ACIUS0_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGB0218342.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26571..26744
                     /gene="yciZ"
                     /locus_tag="ACIUS0_000440"
     CDS             26571..26744
                     /gene="yciZ"
                     /locus_tag="ACIUS0_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGB0218343.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="ACIUS0_000441"
     CDS             26834..27583
                     /locus_tag="ACIUS0_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGB0218344.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="ACIUS0_000442"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="ACIUS0_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGB0218345.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACIUS0_000443"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="ACIUS0_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGB0218346.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACIUS0_000444"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="ACIUS0_000444"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGB0218347.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACIUS0_000445"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="ACIUS0_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGB0218348.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACIUS0_000446"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="ACIUS0_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGB0218349.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACIUS0_000447"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="ACIUS0_000447"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGB0218350.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="ACIUS0_000448"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="ACIUS0_000448"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGB0218351.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACIUS0_000449"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="ACIUS0_000449"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGB0218352.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGGPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACIUS0_000450"
     CDS             complement(35512..35607)
                     /gene="ymiC"
                     /locus_tag="ACIUS0_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGB0218353.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACIUS0_000451"
     CDS             complement(35721..35888)
                     /gene="yciX"
                     /locus_tag="ACIUS0_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGB0218354.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36031)
                     /locus_tag="ACIUS0_000452"
     CDS             complement(35891..36031)
                     /locus_tag="ACIUS0_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGB0218355.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACIUS0_000453"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="ACIUS0_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGB0218356.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACIUS0_000454"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="ACIUS0_000454"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGB0218357.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="ACIUS0_000455"
     CDS             40511..40762
                     /locus_tag="ACIUS0_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGB0218358.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACIUS0_000456"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="ACIUS0_000456"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGB0218359.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="ACIUS0_000457"
     CDS             42067..42825
                     /locus_tag="ACIUS0_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGB0218360.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="ACIUS0_000458"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="ACIUS0_000458"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGB0218361.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACIUS0_000459"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="ACIUS0_000459"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGB0218362.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="ACIUS0_000460"
     CDS             complement(44425..45045)
                     /locus_tag="ACIUS0_000460"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGB0218363.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACIUS0_000461"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(45042..45923)
                     /gene="rnm"
                     /locus_tag="ACIUS0_000461"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGB0218364.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="ACIUS0_000462"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="ACIUS0_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGB0218365.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="ACIUS0_000463"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="ACIUS0_000463"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGB0218366.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="ACIUS0_000464"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="ACIUS0_000464"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGB0218367.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="ACIUS0_000465"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="ACIUS0_000465"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGB0218368.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="ACIUS0_000466"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="ACIUS0_000466"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGB0218369.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="ACIUS0_000467"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="ACIUS0_000467"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGB0218370.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="ACIUS0_000468"
     CDS             53107..53286
                     /locus_tag="ACIUS0_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGB0218371.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="ACIUS0_000469"
     CDS             53372..53872
                     /locus_tag="ACIUS0_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGB0218372.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDHVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="ACIUS0_000470"
     CDS             53918..54424
                     /locus_tag="ACIUS0_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGB0218373.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACIUS0_000471"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="ACIUS0_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGB0218374.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="ACIUS0_000472"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="ACIUS0_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="ACIUS0_000473"
     CDS             55479..56222
                     /locus_tag="ACIUS0_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGB0218375.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="ACIUS0_000474"
     CDS             56252..56791
                     /locus_tag="ACIUS0_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGB0218376.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="ACIUS0_000475"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="ACIUS0_000475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGB0218377.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACIUS0_000476"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="ACIUS0_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGB0218378.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58277..58573
                     /locus_tag="ACIUS0_000477"
     CDS             58277..58573
                     /locus_tag="ACIUS0_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGB0218379.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14060 a        15146 c        14644 g        14724 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggcc cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca cgttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacggttgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
    58141 tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga
    58201 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
    58261 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
    58321 aaaacgcctt tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg
    58381 tcgtttgttg acagctggtc caatgccagc ggtagacagt aacgatccag gggctgcggg
    58441 atttactggc tcaaccgtta ttgctgaatt tgaatcgctg gaagcagctc aggcctgggc
    58501 cgatgctgac ccgtacgtag cggcaggagt ctatgaacac gtttcagtga aaccatttaa
    58561 gaaagtgttc tgag
//
LOCUS       DBBBSB010000008        58559 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.008, whole genome shotgun sequence.
ACCESSION   DBBBSB010000008 DBBBSB010000000
VERSION     DBBBSB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58559
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.008"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            132..2666
                     /gene="mrcB"
                     /locus_tag="ACIUS0_000478"
     CDS             132..2666
                     /gene="mrcB"
                     /locus_tag="ACIUS0_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGB0218380.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2886..5129
                     /gene="fhuA"
                     /locus_tag="ACIUS0_000479"
     CDS             2886..5129
                     /gene="fhuA"
                     /locus_tag="ACIUS0_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGB0218381.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5180..5977
                     /gene="fhuC"
                     /locus_tag="ACIUS0_000480"
     CDS             5180..5977
                     /gene="fhuC"
                     /locus_tag="ACIUS0_000480"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGB0218382.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5977..6867
                     /gene="fhuD"
                     /locus_tag="ACIUS0_000481"
     CDS             5977..6867
                     /gene="fhuD"
                     /locus_tag="ACIUS0_000481"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGB0218383.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6864..8846
                     /gene="fhuB"
                     /locus_tag="ACIUS0_000482"
     CDS             6864..8846
                     /gene="fhuB"
                     /locus_tag="ACIUS0_000482"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGB0218384.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8881..10161)
                     /gene="hemL"
                     /locus_tag="ACIUS0_000483"
     CDS             complement(8881..10161)
                     /gene="hemL"
                     /locus_tag="ACIUS0_000483"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGB0218385.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10327..10374
                     /gene="yadX"
                     /locus_tag="ACIUS0_000484"
     CDS             10327..10374
                     /gene="yadX"
                     /locus_tag="ACIUS0_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGB0218386.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10386..11807
                     /gene="clcA"
                     /locus_tag="ACIUS0_000485"
     CDS             10386..11807
                     /gene="clcA"
                     /locus_tag="ACIUS0_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGB0218387.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11831..11896
                     /gene="yadW"
                     /locus_tag="ACIUS0_000486"
     CDS             11831..11896
                     /gene="yadW"
                     /locus_tag="ACIUS0_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGB0218388.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11889..12233
                     /gene="erpA"
                     /locus_tag="ACIUS0_000487"
     CDS             11889..12233
                     /gene="erpA"
                     /locus_tag="ACIUS0_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGB0218389.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12280..12903)
                     /locus_tag="ACIUS0_000488"
     CDS             complement(12280..12903)
                     /locus_tag="ACIUS0_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGB0218390.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12941..13741)
                     /gene="btuF"
                     /locus_tag="ACIUS0_000489"
     CDS             complement(12941..13741)
                     /gene="btuF"
                     /locus_tag="ACIUS0_000489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGB0218391.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13734..14432)
                     /gene="mtnN"
                     /locus_tag="ACIUS0_000490"
     CDS             complement(13734..14432)
                     /gene="mtnN"
                     /locus_tag="ACIUS0_000490"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGB0218392.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14516..16033
                     /gene="dgt"
                     /locus_tag="ACIUS0_000491"
     CDS             14516..16033
                     /gene="dgt"
                     /locus_tag="ACIUS0_000491"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGB0218393.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16163..17587
                     /gene="degP"
                     /locus_tag="ACIUS0_000492"
     CDS             16163..17587
                     /gene="degP"
                     /locus_tag="ACIUS0_000492"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGB0218394.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17742..18898
                     /gene="cdaR"
                     /locus_tag="ACIUS0_000493"
                     /pseudo
     CDS             17742..18898
                     /gene="cdaR"
                     /locus_tag="ACIUS0_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18987..19373)
                     /locus_tag="ACIUS0_000494"
     CDS             complement(18987..19373)
                     /locus_tag="ACIUS0_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGB0218395.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19687..20511)
                     /gene="dapD"
                     /locus_tag="ACIUS0_000495"
     CDS             complement(19687..20511)
                     /gene="dapD"
                     /locus_tag="ACIUS0_000495"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGB0218396.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20542..23214)
                     /gene="glnD"
                     /locus_tag="ACIUS0_000496"
     CDS             complement(20542..23214)
                     /gene="glnD"
                     /locus_tag="ACIUS0_000496"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGB0218397.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23276..24070)
                     /gene="map"
                     /locus_tag="ACIUS0_000497"
     CDS             complement(23276..24070)
                     /gene="map"
                     /locus_tag="ACIUS0_000497"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGB0218398.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24438..25163
                     /gene="rpsB"
                     /locus_tag="ACIUS0_000498"
     CDS             24438..25163
                     /gene="rpsB"
                     /locus_tag="ACIUS0_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGB0218399.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25421..26272
                     /gene="tsf"
                     /locus_tag="ACIUS0_000499"
     CDS             25421..26272
                     /gene="tsf"
                     /locus_tag="ACIUS0_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGB0218400.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26419..27144
                     /gene="pyrH"
                     /locus_tag="ACIUS0_000500"
     CDS             26419..27144
                     /gene="pyrH"
                     /locus_tag="ACIUS0_000500"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGB0218401.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27436..27993
                     /gene="frr"
                     /locus_tag="ACIUS0_000501"
     CDS             27436..27993
                     /gene="frr"
                     /locus_tag="ACIUS0_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGB0218402.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28085..29281
                     /gene="ispC"
                     /locus_tag="ACIUS0_000502"
     CDS             28085..29281
                     /gene="ispC"
                     /locus_tag="ACIUS0_000502"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGB0218403.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            29470..30228
                     /gene="ispU"
                     /locus_tag="ACIUS0_000503"
                     /gene_synonym="uppS"
     CDS             29470..30228
                     /gene="ispU"
                     /locus_tag="ACIUS0_000503"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGB0218404.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30241..31098
                     /gene="cdsA"
                     /locus_tag="ACIUS0_000504"
     CDS             30241..31098
                     /gene="cdsA"
                     /locus_tag="ACIUS0_000504"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGB0218405.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31110..32462
                     /gene="rseP"
                     /locus_tag="ACIUS0_000505"
     CDS             31110..32462
                     /gene="rseP"
                     /locus_tag="ACIUS0_000505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGB0218406.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32492..34924
                     /gene="bamA"
                     /locus_tag="ACIUS0_000506"
     CDS             32492..34924
                     /gene="bamA"
                     /locus_tag="ACIUS0_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGB0218407.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35046..35531
                     /gene="skp"
                     /locus_tag="ACIUS0_000507"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35046..35531
                     /gene="skp"
                     /locus_tag="ACIUS0_000507"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGB0218408.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35535..36560
                     /gene="lpxD"
                     /locus_tag="ACIUS0_000508"
     CDS             35535..36560
                     /gene="lpxD"
                     /locus_tag="ACIUS0_000508"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGB0218409.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36665..37120
                     /gene="fabZ"
                     /locus_tag="ACIUS0_000509"
     CDS             36665..37120
                     /gene="fabZ"
                     /locus_tag="ACIUS0_000509"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGB0218410.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37124..37912
                     /gene="lpxA"
                     /locus_tag="ACIUS0_000510"
     CDS             37124..37912
                     /gene="lpxA"
                     /locus_tag="ACIUS0_000510"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGB0218411.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37912..39060
                     /gene="lpxB"
                     /locus_tag="ACIUS0_000511"
     CDS             37912..39060
                     /gene="lpxB"
                     /locus_tag="ACIUS0_000511"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGB0218412.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39057..39653
                     /gene="rnhB"
                     /locus_tag="ACIUS0_000512"
     CDS             39057..39653
                     /gene="rnhB"
                     /locus_tag="ACIUS0_000512"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGB0218413.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39690..43172
                     /gene="dnaE"
                     /locus_tag="ACIUS0_000513"
     CDS             39690..43172
                     /gene="dnaE"
                     /locus_tag="ACIUS0_000513"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGB0218414.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43185..44144
                     /gene="accA"
                     /locus_tag="ACIUS0_000514"
     CDS             43185..44144
                     /gene="accA"
                     /locus_tag="ACIUS0_000514"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGB0218415.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44243..46384
                     /gene="ldcC"
                     /locus_tag="ACIUS0_000515"
     CDS             44243..46384
                     /gene="ldcC"
                     /locus_tag="ACIUS0_000515"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGB0218416.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46441..46830
                     /locus_tag="ACIUS0_000516"
     CDS             46441..46830
                     /locus_tag="ACIUS0_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGB0218417.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46895..48193
                     /gene="tilS"
                     /locus_tag="ACIUS0_000517"
     CDS             46895..48193
                     /gene="tilS"
                     /locus_tag="ACIUS0_000517"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGB0218418.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48242..48502)
                     /gene="rof"
                     /locus_tag="ACIUS0_000518"
     CDS             complement(48242..48502)
                     /gene="rof"
                     /locus_tag="ACIUS0_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGB0218419.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48489..48689)
                     /locus_tag="ACIUS0_000519"
     CDS             complement(48489..48689)
                     /locus_tag="ACIUS0_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGB0218420.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48855..49400
                     /locus_tag="ACIUS0_000520"
     CDS             48855..49400
                     /locus_tag="ACIUS0_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGB0218421.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49397..49819
                     /gene="arfB"
                     /locus_tag="ACIUS0_000521"
     CDS             49397..49819
                     /gene="arfB"
                     /locus_tag="ACIUS0_000521"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGB0218422.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49833..50543
                     /gene="nlpE"
                     /locus_tag="ACIUS0_000522"
                     /gene_synonym="cutF"
     CDS             49833..50543
                     /gene="nlpE"
                     /locus_tag="ACIUS0_000522"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGB0218423.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50698..51522)
                     /locus_tag="ACIUS0_000523"
     CDS             complement(50698..51522)
                     /locus_tag="ACIUS0_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HGB0218424.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51575..53293)
                     /gene="proS"
                     /locus_tag="ACIUS0_000524"
     CDS             complement(51575..53293)
                     /gene="proS"
                     /locus_tag="ACIUS0_000524"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGB0218425.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53404..54111)
                     /gene="tsaA"
                     /locus_tag="ACIUS0_000525"
     CDS             complement(53404..54111)
                     /gene="tsaA"
                     /locus_tag="ACIUS0_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGB0218426.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54108..54512)
                     /gene="rcsF"
                     /locus_tag="ACIUS0_000526"
     CDS             complement(54108..54512)
                     /gene="rcsF"
                     /locus_tag="ACIUS0_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGB0218427.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54630..55445)
                     /gene="metQ"
                     /locus_tag="ACIUS0_000527"
     CDS             complement(54630..55445)
                     /gene="metQ"
                     /locus_tag="ACIUS0_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGB0218428.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55485..56138)
                     /locus_tag="ACIUS0_000528"
     CDS             complement(55485..56138)
                     /locus_tag="ACIUS0_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGB0218429.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56131..57162)
                     /gene="metN"
                     /locus_tag="ACIUS0_000529"
     CDS             complement(56131..57162)
                     /gene="metN"
                     /locus_tag="ACIUS0_000529"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGB0218430.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57350..57925
                     /gene="gmhB"
                     /locus_tag="ACIUS0_000530"
     CDS             57350..57925
                     /gene="gmhB"
                     /locus_tag="ACIUS0_000530"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGB0218431.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            58289..>58559
                     /locus_tag="ACIUS0_000531"
     rRNA            58289..>58559
                     /locus_tag="ACIUS0_000531"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        13920 a        14424 c        16100 g        14115 t
ORIGIN      
        1 cctcacccta accctctccc tcaagggaga ggggaccgat cgagcgcaaa ttttgagaga
       61 tatcttcttc tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc
      121 ggagaaaaag catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc
      181 gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt
      241 atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc
      301 gtaagcctcg tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg
      361 ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg
      421 gcaaggtctg gcaactgccc gcggcagttt atggccgaat ggtcaacctt gaaccagata
      481 tgaccatcag caagaacgaa atggtgaagc tgctggaggc aacacagtat cgtcaggtgt
      541 cgaaaatgac ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgatccgcc
      601 gtccgtttga tttcccggac agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg
      661 gtgatcatct ggcgaccatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc
      721 ttgatccgcg tctgatcacc atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc
      781 cgcgcagtgg tttccctgat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt
      841 tttacgagca tgatggaatc agtctctact ccattgggcg tgcggtgctg gcaaacctga
      901 ccgcaggtcg cacggtgcag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt
      961 tcctctccag cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca
     1021 tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg
     1081 gtcagagcgg cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc
     1141 gcccggtaga agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg
     1201 cgtccatcta caacccgtgg cgtaacccga aactggcgct ggagcgacgt aatctggtgc
     1261 tgcgtctgct gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgccc
     1321 gtccgctggg ggttcagccg cgtggtgggg tgatctctcc tcagccagcc tttatgcagc
     1381 tggtgcgtca ggagttgcag gcaaaactgg gcgataaggt aaaagatctc tccggcgtga
     1441 agatcttcac tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag
     1501 gcattccggc actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg
     1561 tcgaccgctt tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg
     1621 gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt
     1681 atctgacggc tttaagccag ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc
     1741 caatcgcgct gcgtcagccg aatggccagg tctggtcacc gcagaacgat gaccgtcgtt
     1801 atagcgaaag cggcagagtg atgctggtgg atgcgttgac acgttcgatg aacgtgccga
     1861 cggtaaatct ggggatggcg ctggggctgc ctgcggttac ggagacctgg atcaaactgg
     1921 gcgtaccgaa agatcagttg catccggttc cggcaatgct gctgggggcg ttgaacttaa
     1981 cgccaatcga agtggcgcag gcattccaga ccatcgccag cggtggtaac cgtgcaccgc
     2041 tttctgcact gcgttcggta atcgcggaag atggtaaggt gctgtatcag agcttcccgc
     2101 aggcagaacg cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg
     2161 tggtacaacg tggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag
     2221 ggaaaacagg gactaccaac aataacgtag atacctggtt tgcaggcatt gacggcagca
     2281 cggtgaccat cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca
     2341 gcggggcaat gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc
     2401 ttgttccgcc agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca
     2461 gcggtggcat gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga
     2521 gcgagatgca gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac
     2581 agccgcaaca gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga
     2641 tcaaggatat gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc
     2701 atccggtaaa ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat
     2761 taacccttca ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat
     2821 tatcttatct ttataataat cattctcgtt tacgttatta ttcactttac atcagagata
     2881 taccaatggc gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg
     2941 tagtagccac agcggttagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag
     3001 aagacactat caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg
     3061 caactattgc ggcgcggcag tcagctaccg gcactaaaac cgatacgccg attcaaaaag
     3121 tgccacagtc tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg
     3181 taaaagaagc gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca
     3241 cctatgacca cctgatcatt cgcggttttg cggcagaagg ccaaagccaa aataactatc
     3301 tgaatggcct gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc
     3361 tggaacgcgc tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg
     3421 gcggtctgtt gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt
     3481 ttaaagccgg tactgacagc ctgttccaga ctggttttga ctttagcgat gcgttggatg
     3541 atgacggtgt ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga
     3601 aagggtcaga agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata
     3661 aaaccaattt caccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct
     3721 ggttgccgaa agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact
     3781 ttaatgaagg ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct
     3841 tcgatcacga atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca
     3901 aaacctcgca aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca
     3961 gcaaacagtg tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg
     4021 tcgttgatga tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg
     4081 ccactggcga tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg
     4141 acatcaacgc ctggtttggt tacgacgact ctgtgccact gttcaatctg tacaatccgg
     4201 tgaatcccga tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc
     4261 tgaataaaca gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc
     4321 tggtcaccct aggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg
     4381 ggacgaccga taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt
     4441 ttgataatgg tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag
     4501 ttgggaagga tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga
     4561 aatatgtacc ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa
     4621 ccaacaacct gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc
     4681 gcgcacgtgg cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg
     4741 gttcttatac ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc
     4801 ctgcacaggt gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc
     4861 cgctttcagg tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc
     4921 cggctaactc ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc
     4981 tggcgcgagt cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc
     5041 gtgaatacgt cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg
     5101 tcgttgcaac cgcaaccttt cgtttctaat ttctcttttg gggcacggat ttccgtgccc
     5161 atttcacaag ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact
     5221 gcgtaatatc tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt
     5281 tcctgccggg aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct
     5341 caaaatgctt ggtcgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc
     5401 gctggaaagc tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct
     5461 tcctccggca gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca
     5521 tggcgcgctg gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct
     5581 ggttggctta aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca
     5641 gcgggcgtgg atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc
     5701 gacttcagcg ctggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag
     5761 tcaggaacgt ggcctgacgg tcattgccgt gttgcacgat atcaatatgg cggcacgcta
     5821 ctgtgattat ctggtcgccc tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga
     5881 aattatgcgc ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca
     5941 tccggcgggt gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg
     6001 cgccgtcgac tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc
     6061 cacgcggcgg ctattgaccc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta
     6121 ctgctggcgc tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg
     6181 gtcagcgaac caccattgcc ggactcagtg attgacgtcg gtttgcgtac agaacctaac
     6241 cttgaactgc tgaccgaaat gaaaccgtcg tttatggtct ggtcggcagg atatggccct
     6301 tcatcagaaa tgctggcacg tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa
     6361 catccgttgg cgatggcgcg taaatcgctg acggaaatgg cagatttact taacctgcaa
     6421 agcgcagcgg aaacgcattt agcgcaatac gaagacttta tccgcagcat gaaaccccgc
     6481 tttgtgaagc gtggtgcacg cccgttactg ctgacgacgc ttatcgatcc gcgccatatg
     6541 ctggtcttcg gtccaaacag cttgttccag gaaattctcg atgagtacgg catcccaaat
     6601 gcctggcaag gggaaaccaa cttctggggc agtaccgccg tcagtatcga tcgtctggca
     6661 gcgtataaag acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg
     6721 ctaatggcaa ccccgctgtg gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc
     6781 gtacccgcag tctggtttta tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg
     6841 gataacgcca tcggaggtaa agcgtgagta aacgaattgc gcttttcccg gcgttattgc
     6901 tggcgctgtt agtgattgtc gctacggcgc tcacctggat gaacttctca caggcgctgc
     6961 cgcgcagtca gtgggcgcag gccgcctggt cgccggatat tgacgtcatc gagcagatga
     7021 tttttcacta cagcttgttg ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg
     7081 ggctggtggg cgtgctgttt cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt
     7141 tgggcgttgc aacaggcgcg caactgggga ttaccgtcac cacgctctgg gcgatcccta
     7201 gcgcgatggc gagccagttt gctgcgctgg caggggcttg cgttgttggc ttaattgtct
     7261 ttggcgtcgc gtgggggaaa cggctgtcgc cggtaacgct gattttcgcg gggttggtag
     7321 tgagccttta ttgcggcgca atcaatcagt tactggttat cttccatcat gaccagttgc
     7381 aaagcatgtt tctgtggagc actggaacgc tgacgcaaac cgactggggc ggtgttgagc
     7441 gtttatggcc gcagttgctg ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa
     7501 ccctgatggg gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc
     7561 gtctggcggc gctgtcgctg gcgattgtca tcagtgcgct gctggtgaac gctgtgggga
     7621 ttatcggctt tatcgggttg ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc
     7681 tgctgccacg actgatgctg gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc
     7741 aaatcatcct ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt
     7801 tgatcggtgc gccgctgcta ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg
     7861 atatgaaggt taacgatcgt gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg
     7921 cgggcggcgt gctgttgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc
     7981 acggctggac gtgggcgagc ggggcgttgc tcgaggattt aatgccctgg cgctggccgc
     8041 gaattatggc ggcgctgttt gcgggcgtca tgctggcggt agcgggctgt attattcagc
     8101 gactgaccgg caacccgatg gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt
     8161 ttggtgtggt gttaatgctg tttctggtac cgggtaatgc ctttggctgg ttgttacccg
     8221 caggcagtct cggggcggcg gtgacgctgt tgatcattat gatcgccgcc ggacgcggtg
     8281 gattttcccc acaccgcatg ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc
     8341 ttttgatgat gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt
     8401 ccggttcgac ctacaacgcg accgatgctc aggtctggcg caccggaatt gtgatggtga
     8461 ttttgctggc gattactccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg
     8521 ataccgcgcg ggcggtaggg atggcgctga cgccgacgcg aattgcgctg ctgctgttag
     8581 cggcttgcct gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa
     8641 tggcaccgca tattgcgcgg atgatgggct ttcgacggac gatgccacac atcgtaattt
     8701 cggcgctggt gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt
     8761 ttccattcca gatcccggcg gggctgctgt cgacctttat cggtgcgcca tattttatct
     8821 atttgctgag aaagcagagc cgttaataga atgcctcgcc cggtgggcga ggcaaaatta
     8881 tcacaacttc gcgaacaccc gacgtgcagc atcgatggtg ttattgatat cttccatgct
     8941 gtgcgccaca gacataaagc ctgcttcaaa cgctgacggt gccaggtaaa caccttcatc
     9001 cagcatcata tggaagaaac gcttaaaacg ctcgacgtca caggccatca catcctgata
     9061 gcacgtcacg gactcggcgt cggtaaagaa gataccgaac ataccaccga cgtggttaac
     9121 gaccagcgga attcccgctt cttctgccgc ttccagcaga ccttctgcca gacgtgttgt
     9181 cagctcatcc agcgtttcgt gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc
     9241 cgctgccatc gcaatcgggt taccggaaag cgtacccgcc tgatagaccg gacctgtcgg
     9301 ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc
     9361 gatgattttt ccgaggcagg ttaaatctgg cactacggcg aaataatcct gtgcgccagc
     9421 tagcgctacg cggaaaccgg tcatcacttc atcgatgatc agcaacgcgc caaattcgtc
     9481 gcacagcgca cgcagacctg gcaggaactc tggcagcggc ggaacacagt tcatattgcc
     9541 tgccaccggc tcgacgataa tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg
     9601 tacagaggcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac
     9661 gcccggcgag tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca
     9721 gtcagcgtga ccgtggtaac aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc
     9781 acgggccagg cggatggcgc tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat
     9841 atccatggtc gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggtcgg
     9901 tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga tcgccggatg
     9961 gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc
    10021 aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc
    10081 agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta
    10141 aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta
    10201 ttccatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc
    10261 aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg
    10321 attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc
    10381 attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca
    10441 gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg
    10501 cggcagtcgt cggcacgctt gtcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct
    10561 ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgctactgt
    10621 taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca
    10681 aatacgcgcc cgaagcaggg ggatcgggga tcccggaaat tgaaggggcg ctggaagatc
    10741 aacgtcctgt tcgctggtgg cgtgtattgc cggtgaaatt ctttggcggg ctggggacac
    10801 tcggcggagg catggggcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatca
    10861 gtcgcatggt gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg
    10921 caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt
    10981 ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat
    11041 ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga
    11101 ttgacgtcgg taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg
    11161 gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt
    11221 tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggg ggggcgattg
    11281 gcggtctgtg tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc
    11341 tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg
    11401 ctcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc
    11461 cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt
    11521 ttccgcaata tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg
    11581 cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact
    11641 accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta
    11701 ccggcgggaa acctctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg
    11761 agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg
    11821 gtattagata atggcgatta ttattgggtt agaatttgcc cgattgccga tgtcgtttgg
    11881 agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca
    11941 aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca
    12001 ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag
    12061 gcgatatgac catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat
    12121 atctggtcgg cggttccgtt gactacaccg aaggactgga aggttctcgt ttcatcgtga
    12181 ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg
    12241 ttcatgtgcc ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag
    12301 cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac
    12361 gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc
    12421 gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg
    12481 gatttcgcgg accagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat
    12541 acaaaccgcg attaacggac cggcttccgc attaaaggct ttattcacgc taatgccgac
    12601 aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg
    12661 ctggcgcacc agcacgatgg tcagcatgct ggtgaccatg gcaacgacca gatcggtggg
    12721 atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc
    12781 tactgcggta accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc
    12841 taacaaaacg ccgtagatgg caaataccgc tgtgccgact atatccagcc aatagacgag
    12901 cattgttcaa tccccactga gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg
    12961 cattacagag ctgttgtgct gcgaggataa tacgtgggct tgcacgttca aaccagtcac
    13021 tcgtgagagg aataacggga attttgagct gttcacccca gtattgtttg attttaggaa
    13081 tttggtccgg tccgcctgta atgacaatag cctgtggcga gcgtgctaac acctgttcgc
    13141 ggctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt
    13201 cgagtacctg gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt
    13261 gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc acgtactgat
    13321 ccagcaggga ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt
    13381 gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc
    13441 ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca
    13501 gatcgggttt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct
    13561 caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc
    13621 cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt
    13681 tgagccacag tggcgcgaga aaagacaggg cgaccagcgc cctgaacagt gatttagcca
    13741 tgtgccagtt tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc
    13801 aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg
    13861 acaacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct
    13921 acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg
    13981 aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag
    14041 gcctcagcgg cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac
    14101 tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag
    14161 acaacgatat cgcccacttt cagcgttggt gccaggccac cggcagaacc ggtgttaata
    14221 atcacatctg gcttgcagtg ttccaacagc agagtggcac ccagcgccgc agcgacttta
    14281 ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg
    14341 caaccgccga gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct
    14401 tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga
    14461 aggcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc
    14521 acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt
    14581 taaaaccgaa catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc
    14641 tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc gcaatgccgc
    14701 cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtgggacgct acatcgccaa
    14761 agaaatttta agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact
    14821 gaccggcccc tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc
    14881 gccgtttggt cattttggcg aagcagcgat aaatgactgg tttcgccaac gtttgcaccc
    14941 ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt
    15001 acgggacggg gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca
    15061 ttttgagggg aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac
    15121 ttgggcacag gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac
    15181 gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta
    15241 tattgcccgc ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg
    15301 gattatggaa gctgccgacg acatctccta ttgtgtggca gaccttgaag atgcggtaga
    15361 gaaaagaata tttaccgttg agcagcttta tcatcatttg cacgaagcgt ggggccagca
    15421 tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa
    15481 tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa
    15541 taaactggta ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg
    15601 aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata
    15661 taaaaatgtc gctgtaaaac atgtgtttag ccatccggat gtcgagcagc ttgaattgca
    15721 gggctatcgg gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt
    15781 atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg
    15841 cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt
    15901 accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgctgca
    15961 ggattatatc agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc
    16021 cgtagaacaa taaccagtct tttgtaaaga cgaacaataa atttttacct tttgcagaaa
    16081 ctttagttcg gaacttcggg ctataaaacg aatctgaaga acacagcaat tttgcgttat
    16141 ctgttaatcg agactgaaat acatgaaaaa aaacacatta gcactgagtg cactggctct
    16201 gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac
    16261 gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt
    16321 cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc gtaatttcca
    16381 gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc
    16441 gttctgccag gggggccagg gcggtaatgg cggcggccag caacagaaat tcatggcgct
    16501 gggttccggc gtcatcattg atgccgataa aggctatgtc gtcaccaaca accacgttgt
    16561 tgataacgcg acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat
    16621 ggttggcaaa gatccgcgct ctgatatcgc gctgatccaa atccagaacc cgaaaaaact
    16681 gaccgcaatt aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat
    16741 tggtaacccg tttggtctgg gcgagacggt aacttccggg attgtctctg cgctggggcg
    16801 tagcggcctg aatgctgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg
    16861 tggtaactcc ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc
    16921 gatcctcgca ccggacggcg gcaacatcgg tatcggtttt gctatcccga gtaacatggt
    16981 gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat
    17041 tatggggact gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg
    17101 tgctttcgta agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg
    17161 tgatgtgatc acctcactga acggtaagcc gatcagcagc tttgccgcac tgcgtgctca
    17221 ggtgggtact atgccggtgg gcagcaaact gaccctgggc ttactgcgcg acggtaagca
    17281 ggttaacgtg aacctggaac tgcagcagag cagccagaat caggttgatt ccagctccat
    17341 cttcaacggc attgaaggcg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt
    17401 gaacaacgtg aagacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat
    17461 tattggtgcg aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag
    17521 caaaccgtct gtgctggcac tgaacattca gcgcggcgac agcaccatct acctgttaat
    17581 gcagtaatct ccctcaaccc cttcctgaat acgggaaggg gttctcctta caatctgtga
    17641 acttcgccac aactccatac atcttcatca tcctttgggc atttgcacaa tgccgtacgt
    17701 tacgtacttc cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg
    17761 ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca
    17821 acgtaatgga tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat
    17881 tgcacgaagg tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg
    17941 tagcacgtca tctgcacggt gtgcggcagg ggattaatct accgttacgg ctggaaggtg
    18001 aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac
    18061 tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc
    18121 aggatagccg tttgcgggaa gaactggtga tgaacctgat tcaggcagag gagaatactc
    18181 ccgcacttct gaatgggcgc aacggctggg gatcgatctc aatcaaccgc gagtggtggc
    18241 tattgttgag gtcgacagcg gtcagcttgg cgtggacagc gcaatggcgg agttacaaca
    18301 actgcaaaac gcgctgacta cgcccgagcg taataatctg gtggcgattg tctcgctaac
    18361 cgaaatggtg gtgttgaaac cggcgttgaa ctcttttggg cgctgggatg cagaagatca
    18421 tcgtaagcga gttgaacaac tgattacccg catgaaagag tacggccagc tgcgttttcg
    18481 cgtttcactg ggcaactatt ttaccggtcc tggcagtatt gcccgatcct atcgtacggc
    18541 gaaaacgacg atggtggtgg gtaaacagcg gatgccagaa agtcgctgct atttttatca
    18601 ggatctgatg ttacctgtgt tactcgacag tttgcgtggc gactggcagg ccaacgaact
    18661 ggcgcgaccg ctggcgcggc tgaaagcgat ggacaataac ggcttgctgc gacgaacgct
    18721 ggcggcgtgg tttcgccaca atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca
    18781 tcgtaatacc ctggagtatc ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg
    18841 caattttgat gacaggttgc tgctgtatgt ggcgttacaa ctggatgaag agcggtagat
    18901 tatgcgttaa ggttgaggcg gcgctggctc attcccctca gcccgaaggg gagaggggaa
    18961 tgcccgagtt aaccgtcaat tcgggattat ttacgcgtta gtttttccag atcggcttca
    19021 atctcgctga tcttattggt tacgactgac tccaggtgac gtaagtcgtc gaggatctta
    19081 cgcttaagat caacttcgct gcggtcacgc tgacagattt gatcaagctc atcaatgata
    19141 taccgtagat tcgggctgat ttcctggact tctttataac cctgacccac accatcagcg
    19201 acgaccgttt tacgctgacg cggatattta aacttaacgc tcttggcgaa aaactcgcct
    19261 ttgtctttct ggaaatagat tttcagaata tcgttgttgg cttcctgccg gaggctgtaa
    19321 cgatcaattt cttcaggatt ggtaataccc agacttttca gattgtcgta catagcgtta
    19381 cctcaaaatg agtcagtaaa ttgtgcttat tttagcattt ggcttggccc gcccggctcg
    19441 actgtgatcg cagaaagcct ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa
    19501 ttacctgctt tattgtggcg atattccatg ggatggatta gcacatttat cttcgttttg
    19561 cgaattacat gacagtccga aacaagatga aaaagtattt aaaaaagggg gaggaagaat
    19621 gagtaggacg aattattctg agccagaaag catacgggca gcatcatgct gcccgtatat
    19681 gtactcttaa tcgatggtac gcagcagttc gttaatgccg actttgccgc gagttttcgc
    19741 gtcaactttc ttaacgataa ccgcacagta gaggctgtat ttgccatctt ttgacggcag
    19801 attacctgaa acaaccacag accccgccgg aacgcgaccg tagtggattt cgccggtttc
    19861 acggtcgtaa atacgggtgc tctgaccaat gtatacgccc atagagatga cggaaccttc
    19921 ttcgacaatc accccttcaa ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat
    19981 aatggtcggg ttagcctgca gcggctccag cacgccgccg atgcccacgc caccggaaag
    20041 gtggacgttt ttaccaatct gcgcacaaga accgacggtc gcccaggtat caaccatggt
    20101 gccttcatca acatatgcgc cgatgttaac gtaagacggc atcagcacgg tgttacgggc
    20161 aataaacgca ccctgacgta ccgccgctgg tggcacaacg cggaagcctt ctttctggaa
    20221 acgtgcttcg tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc ggctttctgc
    20281 cccttcgatc acctgattat cattaatacg gaaagagagc agcaccgctt ttttcaacca
    20341 ctgatgcgtc acccactgac cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag
    20401 cagggcgatc acctgattta ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat
    20461 ctcggcacgg cgttcaaaag cggtttcaat aatgttctgt aactgctgca ttgttaaact
    20521 cttttcatat cagtaaacac atcacccttt atcgtttgga ttgagggcct ctgtcaaccg
    20581 ctgatgcact tcctgctgca actcgttatt aagcgcacgc cggtcagcgg tggcaattat
    20641 gaataaatct tctactcgct cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc
    20701 cagatcggca aaaattttcc cgactcgcgc cagcagtcca ggttggtcga gggcgatcag
    20761 ttcgaggaac gatttgcggt cggtatgggt cggcaaaaac gttacttcgg tttcaacagt
    20821 aaaatggcgt aatttggcgg gttggcgacg gggctgcggt ggctgccagc tactttgcgt
    20881 cagcacctgc tccagaccaa accgaataac ctcatggcga tctgcggaca gcgggctgcc
    20941 atcgggttcc agcacgataa aggtatccat cgccataccg tcgcgagtgg tgaaaatttg
    21001 tgcgtcgtga acacttaaat tgcggcggtc taattcggca cagacggcgg caaacagata
    21061 ggggcggtcc gggctccaga taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag
    21121 caataccagc ggtttgctta aatcatgctg taataaatgg cgggcatgcc aggccagttg
    21181 atttgggcta tggcggacaa aatagttagc acgacagcgt gaccaaattt ggtgcagcgc
    21241 ctcttcgtcg atgttatcca tgcgcagtag tgccagtgcc tggagttggt gatggcgaac
    21301 ccgttcgcgc atatccggcg tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa
    21361 atagagttcg cgcaacagac tttgcttcca gctattccac agtgtttcgt tggtggcgca
    21421 aatgtcagcc actgtcaggc ataccagata gcgcagacga ttttccgttt gaacttcttc
    21481 ggcaaactgc ttgatgactt cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat
    21541 cagcaagtgc tggcgaacca gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg
    21601 gagttctgca aaatgcacta catcctgagc accgagaatg gagtggtcgc cgccgcgtcc
    21661 tttggcgata tcgtgaaaca gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg
    21721 ccagacgtcc acacacaacg gatggcgctg gcgcgtttct tcactggcaa aactctccag
    21781 tttcagcatc acgcggatag tatgttcatc caccgtgtag gcgtggaaca gatcaaactg
    21841 catctgcccg acgatatgcg accattgcgg catatacgcg ccgagcacgc tatggcgatg
    21901 cattggcaat agtccgcgcc gcaccgctcc ggggtgacgc agaatgctca aaaacagttt
    21961 tcgtgcttgc ggaatattac acagcggttg ttgcagatgg cgacgggcat ggcgtaactg
    22021 gcgcagcgtg gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg tgtagaacat
    22081 acgcaagatg gcttccggct ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt
    22141 accgcgtagc tgaaactcat cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag
    22201 gatggcttca tcgaacagtt gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac
    22261 gcggaagtaa tccttcatca tccgctcgac cggttcgttg ccttcaccac tgtaattcag
    22321 acgctgagcg acgctaagtt ggcgatcgaa taacagacga ttatcgtaac ggctgacgac
    22381 cagatgcagg gcaaagcgaa tacgccacag tatatgcaga cattcgttta attccgcccg
    22441 ctccgctgag gttaagaagc caaacccgac catttcatcc agcgatgttg cgccaaaatg
    22501 acggcgggcc acccattgca gagtgtgaat atcgcgcaag ccgccagggc tacttttgat
    22561 gtctggttca aggttgtagc tggtgccatg gtaacgctga tggcgctggt tctgttcttc
    22621 aactttcgct gcgtagaact tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg
    22681 cagttcgagg aagagcgcaa catcgccaat taataagcgg gattcgatta aattggtggc
    22741 gacggttaaa tccgataacc cttccaacat gcactcttca agcgtgcgca cactatgacc
    22801 gacttccagc tttacatccc agagcagcgt taacagctcg cccacttttt gcgcctgatc
    22861 gtccgggagc tttttacggc ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc
    22921 gccacgaccg tagccaccga cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc
    22981 ttcaatccat aatcgttgca ggagctggtc tatgaactcg gtacgtgcct caatcaactg
    23041 ttctgcagag atcccattgt caaaggcatc acccagccaa cgctggaaag tatcgatatg
    23101 ggcttttatc ccacagacgg ttaattcatc acggggccag gcgcatggat tttgcggttg
    23161 accgggcagg gtggagagag cggtgtttgc gtactgttct ggaagggtat tcattgtgcg
    23221 ccacccataa gattaaatta tcgcattaaa aaagccggca ttcgccggct tcatcttatt
    23281 cgtcgtgcga gattatcgcc gggatggtgt catccttgcg tagcgtcaaa atttcgcagc
    23341 cgttatcagt caccacaata gtatgctcat attgtgcaga caagctgcga tctttggttt
    23401 ttaccgtcca gccatctttc atggtgcgga tctctttttt acccgcgttg accattggct
    23461 caatggtgaa cgtcatcccc ggtttcagca cgacgttggt ttcacgggag tcatagtgca
    23521 gcacctgcgg ttcttcatgg aagccgcgac caataccgtg tccgcaatat tcacgaacga
    23581 cggagaagcc ttctgcttcg acaaatttct gaatcgccgc accgatttcg cgcagattaa
    23641 tgcctggttt taccatgcgt agcgccaggt acaggctttc ttgcgtgatg cggcacaggc
    23701 gttcgcccat gatggtcggc ttaccgacga taaacatttt cgaggtatcg ccgtggaaac
    23761 catctttgat tacggtgaca tcaatgttaa cgatatcgcc atctttcagc agcttagcat
    23821 cgtccgggat accgtggcac accacttcat taatagagat gcaaacggat ttcggatagc
    23881 cgtgatagcc gaggcaggca gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga
    23941 tgcgatccag ctcgccggtg ctgacgcccg gtttaacata cggttcgatc atctccagca
    24001 cttcggcagc cagtcggcca gcgacgcgca ttttttcgat atcttctggg gtcttgattg
    24061 agatagccat taattctgtc catcagcgtc ggtgataccg acaatatata tgtaagtgcc
    24121 gtcaatggta tcacacccgg gcaaattgag aatcattctg aatttcgcca aacgtgccac
    24181 tgaaggtttt ctataataga aaattcgacg tctgatgctg tacacagcgc caacaattat
    24241 tggtgtccac gacgtatttg tggtataaag cgcgccggac ttccgatcca tttcgtatac
    24301 acagactgga cggaagcgac aatctcactt tgtgtaacaa cacacacgta tcggcacata
    24361 ttccggggtg ccctttgggg tcggtaatat gggatacgtg gaggcataac cccaactttt
    24421 atatagaggt tttaatcatg gcaactgttt ccatgcgcga catgctcaag gctggtgttc
    24481 acttcggtca ccagacccgt tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc
    24541 gtaacaaagt tcacatcatc aaccttgaga aaactgtacc gatgttcaac gaagctctgg
    24601 ctgaactgaa caagattgtt tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg
    24661 ctgcaagcga agcggtgaaa gacgctgctc tgagctgcga ccagttcttc gtgaaccatc
    24721 gctggctggg cggtatgctg actaactgga aaaccgttcg tcagtccatc aaacgtctga
    24781 aagacctgga aactcagtct caggacggta ctttcgacaa gctgaccaag aaagaagcgc
    24841 tgatgcgcac tcgtgagctg gagaaactgg aaaacagcct gggcggtatc aaagacatgg
    24901 gcggtctgcc ggacgctctg tttgtaatcg atgctgacca cgaacacatt gctatcaaag
    24961 aagcaaacaa cttgggtatt ccggtatttg ctatcgttga taccaactct gatccggacg
    25021 gtgttgactt cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg
    25081 gcgctgttgc tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc caggcggaag
    25141 aaagcttcgt agaagctgag taataaggct tgataactcc cccaaaatag ttcgagttgc
    25201 agaaaggcgg caagctcgag aattcccggg agcttacatc agtaagtgac cgggatgagc
    25261 gagcgaagac aacgcatctg cggcgcgaaa tatgaagggg gagagccctt atagaccagg
    25321 tagtacacgt ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc
    25381 ggtttaatgc cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc
    25441 cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact
    25501 gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat
    25561 taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga
    25621 cggcaactac ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc
    25681 tggtttccag gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga
    25741 cgttgaagtt ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg
    25801 tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca
    25861 gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag agctggttaa
    25921 acacatcgct atgcacgttg ctgcaagcaa gccagaattc atcaaaccag aagacgtatc
    25981 cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa
    26041 gccgaaagaa atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt
    26101 ttctctgacc ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa
    26161 agagcataac gctgaagtga ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa
    26221 agttgagact gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa
    26281 aaggagccgc ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg
    26341 tggggctgcg ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga
    26401 cagtctcagg aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt
    26461 aagttgagtg gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg
    26521 gatcgtatgg ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg
    26581 attggtgggg gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt
    26641 gtgggcgacc acatggggat gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca
    26701 ctgcaccgcg cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc
    26761 gacagctaca gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc
    26821 tccgccggta caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc
    26881 gaaattgaag ccgatgtggt gctgaaagca accaaagttg acggcgtgtt taccgctgat
    26941 ccggcgaaag atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa
    27001 aaagagctga aagtcatgga cctggcggcc ttcacgctgg ctcgtgacca taaattaccg
    27061 attcgtgttt tcaatatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa
    27121 gaagggactt taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt
    27181 aagtatcgcg ggggcgtaag tctggttata aggcgttatt gttgcaggca gtttggtcac
    27241 ggccagcgcg cagcaaccgg agcgtacaaa agtacgtgag gatggcgagc actgctcggg
    27301 gccaaaatgg caaataaaat agcctaataa tccagacgat tacccgtaat atgtttaatc
    27361 agggctatac ttagcacact tccactgtgt gtgactgtct ggtctgactg agacaagttt
    27421 tcaaggattc gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg catggacaaa
    27481 tgcgtagaag cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc
    27541 ctgctggatg gcattgtcgt ggaatattac ggcacgccga cgccgctgcg tcagctggca
    27601 agcgtaacgg tagaagattc ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct
    27661 ccggccgttg aaaaagcgat tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt
    27721 agcgacatcc gtgttccgct gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa
    27781 atcgttcgtg gtgaagcaga acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg
    27841 aacgacaaag tgaaagcact gttgaaagat aaagagatca gcgaagacga cgatcgccgt
    27901 tctcaggacg atgtacagaa actgactgat gctgcaatca agaaaattga agcggcgctg
    27961 gcagacaaag aagcagaact gatgcagttc tgatttcttg aacgacaaaa acgccgctca
    28021 gtagatcctt gcggatcggc tggcggcgtt ttgcttttta ttctgtctca actctggatg
    28081 tttcatgaag caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga
    28141 cgtggtgcgc cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt
    28201 cactcgcatg gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga
    28261 agcgagtgcg aaacttctta aaacgatgct acagcaacag gggagccgca ccgaagtctt
    28321 aagtgggcaa caagccgctt gcgatatggc agcgcttgag gaggttgatc aggtgatggc
    28381 agccattgtt ggtgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac
    28441 cattttgctg gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt
    28501 aaagcagagc aaagcgcaat tgttaccggt cgatagcgaa cataacgcta tttttcagag
    28561 tttaccgcaa cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt
    28621 gtccatttta cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc
    28681 aacaatgacg cccgatcaag cttgccgtca tccgaactgg tcgatgggac gtaaaatttc
    28741 tgtcgattcg gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt
    28801 taacgccagc gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat
    28861 ggtgcgctat caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc
    28921 aattgcccac accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt
    28981 ttgcaaacta agtgcgttga catttgccgc accggattat gagcgttatc catgcctgaa
    29041 actggcgatg gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa
    29101 cgaaatcacc gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt
    29161 gaatttatcc gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt
    29221 atctgttgat gcgaacgcga gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg
    29281 aggataatcc ggctacagag agtcgcgcta tttgttagcg tagggcttca gtgatatagt
    29341 ctgcgccatc tgatcgtaag tagttggctt tataaggtca ggtatgccgt ggttttacac
    29401 ggcttttttt tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa
    29461 aaacgcgtga tgttgtctgc tactcaacca cttagcgaaa aattgccagc gcatggctgc
    29521 cgtcatgttg cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt
    29581 cgtgcctttg ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc
    29641 aacaacggta ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca
    29701 gcgcaggaag tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa
    29761 agtctgcacc gacataacgt gcgtctgcgt attattggcg ataccagtcg ttttaactcg
    29821 cgtttgcaag aacgtattcg taaatctgaa gcgctaacag ccggaaatac cggtctgacg
    29881 ctgaatattg cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg
    29941 gctgaaaagg tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgttaaac
    30001 cagcatgtct gtatgcatga actggcccct gtagatttag taattaggac tgggggagag
    30061 catcgcatta gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttacagat
    30121 gttctctggc ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat
    30181 cgagagcgtc gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt
    30241 ttgctgaagt atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg
    30301 tttctgttgc cgccggtggg gttcgccatt gtaacgctgg tggtctgcat gctggcagcg
    30361 tgggaatggg gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg
    30421 ttatgcgggt tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt
    30481 catcaaccgc tggttgaaat ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta
    30541 ttgctggtgc tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc
    30601 cttatttttg gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc
    30661 tggcactatg acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg
    30721 gtatggggcg ctgactccgg cgcatatatg tttggcaaat tgtttggtaa acataagctg
    30781 gcaccgaagg tttctccggg taaaacctgg caaggcttta taggtggact cgctactgca
    30841 gcggtaatct catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta
    30901 ctcatttgct ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg
    30961 tttaagcgtg aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt
    31021 ttagatcgta ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg
    31081 gtattcagga cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt
    31141 tcatcgttgc actgggtgta cttatcaccg tgcatgaatt tggtcatttc tgggttgccc
    31201 ggcgttgtgg tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc
    31261 gaactgataa gctcggcacc gaatatgtta tcgccctgat cccgttgggc ggttatgtca
    31321 aaatgctgga tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata
    31381 ataaatctgt cggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt
    31441 ttgctatctt tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg
    31501 ttggcgaaat agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac
    31561 taaaagccgt agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg
    31621 ataaaattgg cgatgaaagc accaccatta cagtagcgcc atttgacagc gaccaacggc
    31681 gggatgtaaa gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat
    31741 cttcgctggg gattcgtcct cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc
    31801 caaactcggc ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg
    31861 gtcagccctt aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccgggtaaat
    31921 ccttagcgtt agaaatcgaa aggcagggga gtcccttgtc tttgacatta atcccggaga
    31981 gtaaaccggg taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt
    32041 tgccagatga gtataaagtt gtacgccagt atgggccgtt caacgccatc gtcgaagcca
    32101 cggacaaaac gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg
    32161 gtgatgtgaa actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga
    32221 cagcggaact cggggtagtt tattacctgc cgtttcttgc gcttattagc gtgaacttag
    32281 ggataattaa cctgtttccg ttgcccgtac ttgacggggg gcatctgctg ttccttgcga
    32341 tcgaaaagat caagggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg
    32401 gctcgattct gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat
    32461 gagagttagt taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc
    32521 gctgctgttt agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca
    32581 tttcgaaggc cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac
    32641 aggcgacacg gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg
    32701 caactttgag gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga
    32761 acgtccgacc attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct
    32821 gaagcaaaac ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat
    32881 tgccgatatc gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag
    32941 cgttaaagct gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca
    33001 ggaaggtgtg tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac
    33061 cgacgaactg atctctcatt tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg
    33121 cgatcgtaaa taccagaaac agaaactggc gggcgatctt gaaaccctgc gcagctacta
    33181 tctggaccgc ggttatgccc gtttcaacat cgactctacc caggtcagtc tgacgccaga
    33241 taaaaaaggt atttacgtca cggtgaacat caccgaaggc gatcagtaca agctttctgg
    33301 cgttgaagtg agcggcaacc ttgccgggca ctccgctgaa attgagcaac tgactaagat
    33361 cgagccgggc gagctgtata acggcactaa agtgaccaaa atggaagatg acattaaaaa
    33421 gcttctcggt cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg aaattaacga
    33481 tgccgacaaa accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg
    33541 taagatccgt tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg
    33601 tcagatggaa ggtgcatggt tggggagcga tctggtcgat cagggtaagg agcgtctgaa
    33661 tcgtctgggc ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga
    33721 ccaggttgat gtagtctaca aggtgaaaga acgtaacact ggtagcttca acttcgggat
    33781 tggttacggt actgaaagtg gcgtgagctt ccaggctggc gtgcaacagg ataactggtt
    33841 aggtacaggt tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga
    33901 actgtcggta accaacccgt acttcaccgt agatggcgta agcctcggtg gtcgtctctt
    33961 ctataatgac ttccaggcag atgacgccga cctgtccgac tataccaaca agagttatgg
    34021 tacagacgtg acgttgggct tcccgattaa cgaatataac tcgctgcgtg caggtctggg
    34081 ttatgtacat aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta
    34141 ctctatgggt gaacatccga gcacctctga tcaggataac agcttcaaaa cggacgactt
    34201 cacgttcaac tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg
    34261 ttcacgtgtc aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa
    34321 agtgacgtta gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct
    34381 gggtcgtacc cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga
    34441 gaacttctat gccggtggtt ccagcaccgt gcgtggcttc cagtccaata ccattggtcc
    34501 gaaagcagtt tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg
    34561 tgcgactcag gacggcgcga aagacctgtg taaatcggat gatgctgtag gcggtaacgc
    34621 catggcggtt gccagcctcg agttcatcac cccgacgccg tttattagcg ataagtatgc
    34681 taactcggtt cgtacttcat tcttctggga tatgggtacc gtttgggata caaactggga
    34741 ttccagccaa tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc
    34801 gggtatcgca ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc
    34861 gttcaaaaag tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg
    34921 gtaagtgttc tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata
    34981 taagatcgcc gggccacgca aagaactgca ccctccggtg caaatgggat ggtaaggagt
    35041 ttattgtgaa aaagtggtta ttagctgcag gtcttggttt agcactggca acttctgctc
    35101 aggcggctga caaaattgca atcgtcaata tgggcagcct gttccagcag gtagcgcaga
    35161 aaaccggtgt ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc
    35221 gtatggaaac cgatctgcag gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg
    35281 atcgcaccaa actggaaaaa gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc
    35341 aggcttttga gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc
    35401 gtatccagac tgctgtgaaa tccgttgcca acagccagga tatcgatctg gttgttgatg
    35461 caaacgccgt tgcttacaac agcagcgatg taaaagacat cactgccgac gtactgaaac
    35521 aggttaaata agtaatgcct tcaattcgac tggctgattt agcgcagcag ttggatgcag
    35581 aactacacgg tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa
    35641 caggtcacat tacgttcatg gttaacccaa aataccgtga gcatttaggc ttgtgccagg
    35701 cgtccgcggt tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag
    35761 tgaagaatcc ctacctgact tacgcgcgca tggcgcaaat tttagatacc acgccgcagc
    35821 ccgcgcagaa cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg
    35881 tatcgattgg cgctaacgcg gtgattgagt ccggcgttga actgggcgat aacgtgatta
    35941 tcggtgccgg ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg
    36001 cgaacgtaac catttaccat gagatccaga tcggtcagaa ttgcctgatc cagtccggaa
    36061 cagtggtagg cgcagacggc tttggttatg ccaacgatcg tggtaactgg gtgaagatcc
    36121 cacagattgg tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc acaaccatcg
    36181 atcgcggcgc gctggatgac actgttattg gcaatggcgt gatcattgat aaccagtgcc
    36241 agattgcaca taacgtcgtg attggcgaca atacggcggt tgccggtggc gtcattatgg
    36301 cgggcagcct gaaaattggt cgttactgca tgatcggcgg agccagcgta atcaacgggc
    36361 atatggaaat atgcgacaaa gtgacggtta cgggcatggg tatggtgatg cgtcccatca
    36421 ctgaaccagg cgtctattcc tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa
    36481 ccgctgcact ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg cttgagcgca
    36541 aggttaatca acaagactaa cgttccatct tttgttcgcc aaactttacg gcctgtctca
    36601 ttcttacgat tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg acaggaagag
    36661 tatcttgact actaacactc atactctgca gattgaagag attttagaac ttctgccgca
    36721 ccgtttcccg ttcttactgg tggatcgcgt gctggatttt gaagaaggtc gttttctgcg
    36781 cgcagtaaaa aatgtctctg tcaatgagcc attcttccag ggccatttcc ctggaaaacc
    36841 gattttcccg ggtgtgctga ttctggaagc aatggcacag gcaacaggta ttctggcgtt
    36901 taaaagcgta ggaaaactgg aaccgggtga gctgtactac ttcgctggta ttgacgaagc
    36961 gcgcttcaag cgcccggtcg tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa
    37021 aacgcgccgc ggcctgaccc gttttaaagg ggttgctctg gtcgatggta aagtagtttg
    37081 cgaagcaacg atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg ataaatccgc
    37141 ctttgtgcat ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga acgcacacat
    37201 tggtcctttt tgtatcgttg gaccccatgt cgaaattggt gagggtaccg tactgaaatc
    37261 tcacgttgtc gtgaatggtc atactaaaat tggccgcgat aatgagattt atcagtttgc
    37321 ctccatcggc gaagttaacc aggatttgaa atatgctggc gaaccgaccc gtgtggaaat
    37381 cggcgatcgt aaccgcattc gcgaaagcgt caccattcat cgtggcacag tccagggcgg
    37441 tggattgacg aaggtgggca gcgacaactt actgatgatc aacgcgcaca ttgcgcacga
    37501 ttgtacggta ggtaaccgct gtattctcgc caacaacgca acgctggcgg gtcacgtatc
    37561 ggttgacgac ttcgcgatca tcggcggcat gaccgcagtc catcagttct gcatcattgg
    37621 tgcgcatgtg atggttggcg gctgctccgg tgtggcgcag gacgtccctc cttatgttat
    37681 tgcgcagggt aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg
    37741 attcagccgt gaggcgatta ccgctatccg caatgcgtat aagctaattt atcgtagcgg
    37801 taaaacgctc gatgaagtga aaccggaaat tgctgaactg gcggaaacat atccggaagt
    37861 gaaagccttt accgatttct ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac
    37921 cagcgtccat taacgattgc cctggtcgcc ggagaaacct ccggcgatat cctgggggcc
    37981 ggtttaatcc gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg tgttgccggg
    38041 ccacgaatgc aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg
    38101 ggcattgttg aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca
    38161 aagcgttttg gcgaactgaa gccagatgtt tttgttggta ttgatgcgcc cgacttcaat
    38221 attactcttg aaggtaacct caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca
    38281 tccgtctggg cgtggcgaca gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg
    38341 ctcgcatttc tgcctttcga aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt
    38401 atcggtcata ccatggctga tgccatgccg ttagatccag ataaaaatgg tgcccgtgat
    38461 gtgctgggga tcccttacga tgcccactgt ctggcattgt tgccgggcag ccgtggtgca
    38521 gaagtcgaaa tgcttagtgc cgatttcttg aaaactgccc agcttttgcg ccagacatat
    38581 ccggatctcg aaatcgtggt gccgctggtg aatgccaaac gccgcgagca gtttgaacgc
    38641 atcaaagctg aagtcgcgcc agacctgtcg gttcatttgc tggatggaat gggccgtgag
    38701 gcgatggtcg ccagcgatgc ggcactactg gcgtcgggga cggcagccct ggagtgtatg
    38761 ctggcgaaat gcccgatggt ggtgggatat cgcatgaagc cttttacctt ctggttggcg
    38821 aagcggctgg tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg cagagagtta
    38881 gtcaaagagt tattgcagga agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg
    38941 ctgttggcga acgggaaaac cagccacgcg atgcacgata ccttccgtga actgcatcag
    39001 cagatccgct gcaatgccga tgagcaggcg gcacaagccg ttctggagtt agcacaatga
    39061 tcgaatttgt ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc
    39121 cgttagttgg cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc ccgattgccg
    39181 ggctgaatga ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctat gaagagatca
    39241 aagagaaagc gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc gacgagctga
    39301 acattcttca tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg catattgcgc
    39361 cggaatatgt gttgattgat ggtaaccgct gcccgaaatt accgatgcct gcgatggctg
    39421 tggtgaaagg ggatagccgc gtaccagaaa tcagtgccgc gtctatcctg gcgaaagtga
    39481 cgcgtgacgc cgaaatggcg gcgctggata ttgttttccc gcaatatggt tttgcccaac
    39541 ataaagggta tccaaccgct tttcatctgg aaaaactggc tgaatatggc gcgactgaac
    39601 atcatcgtcg cagctttggg cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt
    39661 gtcgagatta agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta cacctgcggg
    39721 tgcacagcga ctactcgatg atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg
    39781 cggcggcgtt gggtatgcca gcactggcga tcaccgattt caccaacctt tgcggtctgg
    39841 tgaagttcta cggagcggga catggcgcag ggattaagcc tatcgtcggg gcagatttta
    39901 atgtccagtg cgacctgctg ggtgatgagt taacccacct gacggtactg gcggcgaaca
    39961 ataccggcta tcagaatctg gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg
    40021 ccgccgggcc gatcatcgat cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc
    40081 tttccggcgg gcgcatgggc gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg
    40141 tagatgagtg tgtcgcgttt tatgaagaac acttcccgga tcgctatttt ctcgagctga
    40201 tccgcaccgg caggccggat gaagaaagct atctgcacgc ggcggtggaa ctggcggaag
    40261 cgcgcggttt gcccgtcgtg gcgactaacg atgtgcgctt tatcgacagc agcgactttg
    40321 acgcacacga aatccgcgtc gcgatccacg acggctttac cctcgacgat cctaaacgcc
    40381 cgcgtaacta ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt gagctgtttg
    40441 ccgacatccc agaagccctt gccaacaccg ttgagatcgc caaacgctgt aacgtaactg
    40501 tgcgtcttgg tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc accgaagatt
    40561 atctggtcaa gcgtgcaaaa gaaggcctgg aagagcgtct ggcattttta ttccctgacg
    40621 aggaagaacg tcttaaacgc cgcccggaat atgacgaacg tctggagact gaacttcagg
    40681 ttatcaacca gatgggcttc ccgggctact tcctcatcgt tatggaattt atccagtggt
    40741 cgaaagataa cggcgtaccg gtagggccag gccgtggctc cggtgcgggt tcactggtgg
    40801 cctacgcgct gaaaatcacc gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt
    40861 tccttaaccc ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt atggagaaac
    40921 gcgatcaggt tatcgaacac gtggcggaca tgtacggtcg tgatgcggta tcgcagatta
    40981 tcaccttcgg tacgatggcg gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc
    41041 atccgtacgg ctttgtcgac cgtatctcga aactgatccc gcccgatccg gggatgacgc
    41101 tggcgaaagc gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg gatgaagaag
    41161 ttaaggcgct gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc
    41221 acgccggtgg ggtggttatc gcgccgacca aaattaccga ttttgcgccg ctttattgcg
    41281 atgaagaggg caaacatccg gtcacccagt ttgataaaag cgacgttgaa tacgccggac
    41341 tggtgaagtt cgacttcctt ggtttgcgta cgctcaccat catcaactgg gcgctggaga
    41401 tgatcaacaa gcggcgggcg aagaatggcg agccaccgct ggatatcgcc gcgatcccgc
    41461 tggatgacaa gaaaagcttc gacatgctgc aacgctcgga aaccacggcg gtattccagc
    41521 ttgaatcgcg cggcatgaag gacctgatca agcgtctgca acctgactgc ttcgaagata
    41581 tgatcgcact ggtggcactg ttccgccctg gtccgttgca gtcagggatg gtggataact
    41641 ttatcgaccg taaacatggt cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg
    41701 aaagcctgaa atcggtactg gagccaacct acggcattat cttgtatcag gaacaggtca
    41761 tgcagattgc ccaggtgctt tctggttata ccctcggtgg cgcggatatg ctgcgccgtg
    41821 cgatgggtaa gaaaaagccg gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg
    41881 cagaaaagaa cggaatcaac gccgaactgg cgatgaaaat cttcgacctg gtggagaaat
    41941 tcgcgggtta cggatttaac aaatcgcact ctgcggccta tgctttggtg tcatatcaaa
    42001 cgttatggct gaaagcgcac tatccggcgg agtttatggc ggcggtaatg accgccgata
    42061 tggacaacac cgagaaggtg gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa
    42121 tcctgccacc agatataaac tccggtcttt accatttcca cgtcaacgac gatggcgaaa
    42181 tcgtgtatgg tattggcgcc atcaaagggg taggtgaagg tccgattgag gccatcatcg
    42241 aagcccgtaa taaaggcggc tacttccgcg aactgtttga tctctgcgcc cgtaccgaca
    42301 ccaaaaagtt aaaccggcgg gtgctggaaa aactgatcat gtccggggcg tttgaccgtc
    42361 ttgggccaca ccgcgcggcg ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc
    42421 aacacgcgaa agcggaagct atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc
    42481 cggaacaaat tgaacaatcc tacgccagct gccaaccgtg gccggagcag gtggtattag
    42541 atggggaacg tgaaacgtta ggcctgtacc tgaccggaca ccctatcaac cagtatttaa
    42601 aagagattga gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg
    42661 gtaaagtcat cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg
    42721 gcaatcgtat cggtatctgc acgctggatg accgttccgg gcggctggaa gtgatgttgt
    42781 ttactgacgc cctggataaa taccagcaat tgctggaaaa agaccgcata cttatcgtca
    42841 gcggacaggt cagctttgat gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga
    42901 tggatattga cgaagcccgg gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca
    42961 ggcaaattga tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc caccgctctg
    43021 ggacaattcc agtacatctc tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg
    43081 gcgcgacgtg gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt ggcctcattg
    43141 gttcggagca ggtggaactg gagtttgact aatacaggaa tactatgagt ctgaatttcc
    43201 ttgattttga acagccgatt gcagagctgg aagcgaaaat cgattctctg actgcggtta
    43261 gccgtcagga tgagaaactg gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa
    43321 aaagcgtaga actgacgcgt aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac
    43381 tggcacgcca tccgcagcgt ccttataccc tggattacgt tcgcctggca tttgatgaat
    43441 ttgacgaact ggctggcgac cgtgcgtatg cagacgataa agctatcgtc ggtggtatcg
    43501 cccgtctcga tggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag
    43561 aaaaaattcg ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc
    43621 tgatgcaaat ggctgaacgc tttaagatgc ctatcatcac ctttatcgac accccggggg
    43681 cttatcctgg cgtgggcgca gaagagcgtg gtcagtctga agccattgca cgcaacctgc
    43741 gtgaaatgtc tcgccttggc gtaccggtag tttgtacggt tatcggtgaa ggtggttctg
    43801 gcggtgcgct ggcgattggc gtgggcgata aagtgaatat gctgcaatac agcacctatt
    43861 ccgttatctc gccggaaggt tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc
    43921 tggcggctga agcgatgggt atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact
    43981 ccatcatccc ggaaccgctg ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt
    44041 tgaaagcgca actgctggcg gatctggccg atctcgatgt gttaagcact gaagatttaa
    44101 aaaatcgtcg ttatcagcgc ctgatgagct acggttacgc gtaattcgca aaagttctga
    44161 aaaagggtca cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg
    44221 aaggattttc caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt
    44281 ttataaagat gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat
    44341 tatctggcca caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg
    44401 cggcgtgatt tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact
    44461 taatgaatat ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt
    44521 gcaggatatg cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat
    44581 cgccattcgt atgcgtcagt acaccaacga atatcttgat aacattacgc cgccgttcac
    44641 gaaagccttg tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat
    44701 gggcggcacc gcatatcaaa aaagcccggt tggctgtctg ttttatgatt ttttcggcgg
    44761 gaataccctt aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgcttgacca
    44821 caccgggcca cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag
    44881 ttatatcgtt accaacggaa tatcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc
    44941 atccggcagt acgctgttga tcgaccgcaa ctgccataaa tcactggcgc atctgttgat
    45001 gatgaacgat gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattctggg
    45061 ggggatcccg cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccactac
    45121 gcaagcacaa tggccggttc atgcggtgat caccaactcc acctatgatg gtttgctcta
    45181 caacaccgac tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg
    45241 ggtgccgtat actcattttc atccaatcta ccagggtaaa agtggtatga gcggcgagcg
    45301 tgttgcggga aaagttttct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc
    45361 gcaggcttca ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt
    45421 tatgatgcat accaccacct cgcccagtta tcccattgtt gcttcggtta agacggcggc
    45481 ggcgatgctg cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct
    45541 gcattttcgc aaagaggtcc agcggctgcg ggaagagtct gacggttggt ttttcgatat
    45601 ctggcaaccg ccgcaggtgg atgaagccga atgcaggccc gttgcgcctg gcgaacaatg
    45661 gcacggcttt aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat
    45721 tttgacaccg gggatggacg aacagggcaa tatgagtgag gaggggatcc cggcggcgct
    45781 ggtagcaaaa ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct
    45841 gctgtttctc tttagtattg gcatcgataa aaccaaagca atgggattat tgcgtgggtt
    45901 gacggaattt aagcgctctt acgatctcaa cctgcggatc aaaaatatgc tgcccgatct
    45961 ctatgctgaa gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat
    46021 ccataagctg attcgtaaac acgatctttc cggtttgatg ttgcgggcat tcgatacttt
    46081 gccggaaatg atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga
    46141 aaccattgcg ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc
    46201 accgggcgta ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact
    46261 cgattttcta ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat
    46321 tcacggcgcg aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg
    46381 ataacttgcc agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt
    46441 atgctgggtt taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc
    46501 aaagctttct actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg
    46561 cgcgactcgt ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct
    46621 tttccgttcc cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct
    46681 tttagcgttg atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt
    46741 gaagccatcc gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac
    46801 gggctgccgt tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac
    46861 gtgccgggca ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact
    46921 tctcacctca cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct
    46981 gcatcagttg gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt
    47041 gcatcacggt ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca
    47101 acagtggcag gtgccgctgg tggtcgaacg cgtacaactt gcgcaagagg ggctggggat
    47161 tgaggcccag gcgcggcagg cacgttatca ggcatttgcc cgtaccctat tgcccggcga
    47221 agtgctggtc accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa
    47281 acgcggtagc ggccctgccg ggctttcggc tatggcggaa gtttcggagt ttgctggaac
    47341 acggcttatc cgcccgttgc ttgcccgcac gcggggggaa ctggagcagt gggcattggc
    47401 gcatggttta cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt
    47461 tctgcgcctg cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg cggaagcaac
    47521 ggcccgcagc gccacacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga
    47581 tgatttagca cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat
    47641 gagtgatgcc cgtcgcgcgg cgattattcg ccgctggctg gcagggcaga atgcaccgat
    47701 gccttcccgc gacgcgctgg tgcgcatctg gcaggaagtg gcgctggcgc gggaagatgc
    47761 ttccccctgt ttacgtttgg gcgcctttga aatccgccgc tatcagtcgc aactgtggtg
    47821 gattaaatcc gtcaccgggc aaagcgaaac cattgtgctg tggcagacgt ggctacaacc
    47881 gctggaatta ccggcggggc tgggaaccgt acagcttact gcgggaggcg atattcgccc
    47941 tccgcgtgca gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt
    48001 cgggcgcaac ggcggacgta agctgaagaa aatctggcaa gagttaggcg tgccgccgtg
    48061 gctacgtgac accacgccac tgctgtttta tggagaaacg ctgattgcgg cggcaggggt
    48121 atttgtgacg caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa
    48181 aacgcttagt taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca
    48241 atcaggattc gcttaccacc accgtaccga tttcagggtg gctaaagctg gtaattttat
    48301 ccagacgcag ctcgcgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc
    48361 gggagactaa atcactggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca
    48421 gcattaaatg atgctggcag gcgagctcaa gattatcgta atcatcacaa ttaattggtt
    48481 gatacgtatc attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct
    48541 ttttccctga cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa
    48601 acgcagccca gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc
    48661 ttgcgtatta actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg
    48721 tggatgcaag tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact
    48781 catttctgca atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg
    48841 aaaggagttt tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg
    48901 atctcgaccg caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa
    48961 cccaggagcg tatgatgctg cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc
    49021 aatttacccg tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc
    49081 tgggcattga tttgtggatt gagctggggc tgccggatga gcgacgaatt aagaaagcct
    49141 gcacccaggc cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt
    49201 ggcagcaaaa tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg
    49261 atgaacaact ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga
    49321 ttcaggatgg tgttatctgg ttatcggatg ataagaataa tctggaagtt aacttaaccg
    49381 cctggcaaca accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct
    49441 tgagatcacc gccattcgtg cgcagggcgc tggcgggcag catgttaata agacctcaac
    49501 ggctattcat ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg
    49561 tctgctcgcc gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca
    49621 ggaataccgc agtcaggaac tgaaccgcga agccgctctg gcccggctgg tggcagtgat
    49681 taaagattta acaaaagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa
    49741 agagcgcagg ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt
    49801 acgcagcggt cgggaataaa aagaaggaat ggatggtgaa aaaagcgata gtgacagcga
    49861 tggctgtaat cagcctcttt actctgatgg gatgtaataa tcgggccgaa gtcgatacgc
    49921 tttctccggc gcaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctac
    49981 cgtgtgccga ttgcgaagga atcgaaatct ctctgttcct cgaaaaagac ggaacatggg
    50041 tgatgaatga gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta
    50101 catgggcgcg aaccgctgac aagctggtat taaccgacag caaaggtgaa aagtcatatt
    50161 atcgggcgaa aggcgatgcg ctggagatgc tcgatcgtga aggcaatcca attgaatcgc
    50221 agttcaacta tacgctggaa ccggcacaat ccagtttacc tatgacgccg atgaccctgc
    50281 gaggcatgta tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac
    50341 gtttcatggt agcgaataac gcagagctgg agcgtagcta cctggctgcg cgcggtcaca
    50401 gtgaaaaacc ggtgttactg tcagtagaag gtcactttac gcttgaggct aatccggata
    50461 ctggtgcgcc aaccaaagta ttagcgcccg atacggcggg taaattttac ccgaataagg
    50521 attgcagtag tttggggcag taaacctgct taaggcagaa acaaacgcaa aattgcctga
    50581 tgcgctgcgc ttatcaggcc tacagcatat aatgcaacat tttgaattag cacgattttg
    50641 taggccggat aaggcgttta cgccgcatcc ggcattttgt gcacgaggct ggctttatca
    50701 ctgagtcagc ccaacaaagc gacccgcctt gatataaatc ccaggcttca ggtgcccaac
    50761 atactgtaac tgcgtttctg gcttaaacgc agagacatca ccaatacgca tatgcatcag
    50821 atcggcaggg ctaatccagc ccgattgcgc cagtgtaagt ggatgcccgg atttggcaaa
    50881 tgcatcagtg acaaattccg aacaaaacca cgactttttg tctccttcgc acacactgct
    50941 taactgcgct ttcgccaggt cgctgacgca ctgttggcga aaatcctcgg agaacggatt
    51001 cagtgagcac atctggcgag tcaccataaa gggaataaat tcgacaatgc cgcgatagtt
    51061 ataaccgcta tctttgattt tattggcaaa cgcggtgata tctgtggctt gttgcggggt
    51121 aagatccggg actcgtaaga cgaaaagctt atcactatgc ttcatcgctt ttttaaggga
    51181 aacaatctgg acgccagcgc ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc
    51241 aacgtgactc acagaggaag tgctgaagac gcggattcca aatgaggtta ccccaaggct
    51301 tgaggagaac agcaaatcgc cgggtttgag atcaggtgcc gttatttctt ttattgattg
    51361 ttcggtgaaa gagctttgat gctggaattt gacagcccat gtttttgcct cggcatctac
    51421 tgctgtggct gatgggtctg gctggctgat atcaacggta caagccgaaa gtaaaagaaa
    51481 gctggggaga agcaggcggc agtacgcctt tggtttatcc attttataca atccatgtaa
    51541 aaaagggccc tgaaattcag gaccctttct ggcatcagcc tttaatctgt ttcaccagat
    51601 attcgacgat gtcaccagtc ttaattaact gtttctcgcc gttgcgacga tatttatatt
    51661 cgatatcgtc gttgtcgagg ttacggtcgc ccagcacaat agtgtgcgga ataccgatca
    51721 gttccatatc agcaaacatc acgcccggac gctctttgcg gtcatccagc agcacttcga
    51781 taccttgtgc acgcagttcg ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt
    51841 tgtgcatgtt catcggcaga atcgccacct ggaacggcgc gatagcgtca ggccatacga
    51901 tgccgcgttc gtcgtagttc tgctcaatcg ccgcagctac cacacgcgtt accccgatac
    51961 cgtagcaacc catcgtcagg atttggttac ggccatcttc accctgtacg gaggctttca
    52021 gtgcttcgga gtacttggta cccagctgga agatgtgacc aacttcgata ccacgtttga
    52081 tcagcagcgt accctggcca tccgggcttg gatcgccagc caccacgtta cggatatctg
    52141 caacttccgg ggtagcgaca tcgcgatccc agttgatacc gaagtagtgt ttaccatcga
    52201 tgttagcacc agcagcgaaa tcactcatcg ccgcaacggt acggtcaatc accaccggaa
    52261 tcggcatgtt taccggaccc agtgaacccg gaccggcttt aaccacagca cgaatttctt
    52321 cttcggtcgc gaaagtcagc gggcttgcaa cctgcggcag tttttctgct ttaacttcgt
    52381 tcagctcgtg atcaccgcgc accagcagcg caaccagcgg gaagctactg ccttcaaccg
    52441 ctttaaccag cagagtctta accgttttct caatcggcag attgaactgt tcaaccagtt
    52501 ccgcgatggt tttcgcgttc ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc
    52561 gcggttcttt cggcgcgata gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt
    52621 cggagaagac cacatcgtct tcaccgctct gcgccagcac ctggaattcg tgagaggcgc
    52681 tgccgccgat agaaccggtg tcggcctgta cggcgcggaa atccagcccc atgcggctga
    52741 agattttgct gtaggccgca tacattgcat cgtaggtttc ctgcagggat tcctgagaag
    52801 tatggaaaga gtaagcatct ttcatcagga attcgcggga acgcatgacg ccgaaacgcg
    52861 gacgcacttc gtcgcggaac ttggtctgga tctgatagaa gttcagcggc agctgtttgt
    52921 aagagctaag ctcgttacga atcaggtcag tgataacttc ttcatgagtt gggccgagta
    52981 cgaacggacg ctcgccacgg tcaacaaaac gcagcagttc cggaccgtac tgttcccaac
    53041 gaccactctc ttgccacaaa tcggctggct gaaccaccgg catcgacacc tcgatcgcac
    53101 cggcgttgtt catctcttca cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg
    53161 tcggcagcca ggtatataac ccggaggcca gcttgcggat catcccggcg cgcagcatca
    53221 gctgatggct gatcacctcg gcgtcggcag gtgtctcctt gagagtggag agcaggtatt
    53281 ggctagtacg catgttgtta cggttccagt tggaaggtag aacaggctca aggcgagcct
    53341 gggacaaaaa aagtgattta gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg
    53401 aatttaacgc ggttccagcg caaagacttc aaaacctgcg tcggtgacgc gccagcgaac
    53461 gttaaaatca tgtagccaga cggcataggt tttgcccgtt tcctcacctt tacgataggc
    53521 cgggcgcggg tcctgcgcca gtacttcgcg gataaacagc gttaactgcg gataacgctt
    53581 ctccagcgtc aaaagctgct tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc
    53641 tggcgcactt tgcgcatagc tggcactggc atcgggaagc gattcggcaa aggggagata
    53701 cggtttgata tccactaccg gcgtaccatc gaccagatcc agactgccga gcttcagaat
    53761 cacgctgcct ttatggcaaa caacctcttt cagctctacc agcgacatgc caattgggtt
    53821 agggcggaaa gtagagcgtg ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg
    53881 tggacgcaca gtcggacgcc agccgccttc catcgtttga tgaaagacga aaaggatcca
    53941 taaatggctg aatgcttcca ggccgcgaac ggcgtcggcc tggttgtagg aagctatgag
    54001 atgcagttcg ccgttggcgc tttttaccag acctggctgg cgcggaacgg cgaatttttc
    54061 tttatagggc gagcgaataa cgcctatttg ctcgaactgg aaactgctca tttcgccgta
    54121 atgttaagcg cagagccgat acatacagcc tgacgatagc agcccggcgt accgctggtg
    54181 acttcgcagc tatgcagtaa tacagcattg gctttcattt tagaggcatt gatttgcatc
    54241 tgcttacgtg cggttggaat gctcggcgga gagtcctgat tagaggcctg gcaagagtcg
    54301 ccactgactt caccgagatc gcggaacggt ttgccgacta attcttctgc attggtataa
    54361 attcggaccg gcgtggcgcg cggcgctttc ggttttgcag gctccgcttt cggctggggt
    54421 gcagtgcttt gaacgggttc gacaggggat ctgcttaaca tggaacagcc gcttagcatg
    54481 agtgctacta aacagatcgg taaagcacgc atagtatttc ctcaatgtat gatcaaaacg
    54541 tcaatattga atcaggagct tgtaaaaatg acaagacggg caagcgcccg tcctgaatga
    54601 tattacaaat tgtggaaaca gcctaaaaat taccagcctt taacagcgcc gccgttaaac
    54661 actttgtttg ctgcttcgta aacttcatca gactgataag cctggacgaa tttcttcacg
    54721 ttctcggcgt ctttgttatc ttcacgcgtt acgatcaggt ttacgtatgg ggagtcttta
    54781 tcttcgacaa agataccgtc tttcgccgga gtcaggccaa tctggctggc ataggtggta
    54841 ttgataactg ccagagcgat ttgcgcgtcg tccagagaac gcggcagttg cggtgcttcc
    54901 agttcaacaa ttttcagatt ttttgggttc tcaacaacat caagaacggt cggcagcagg
    54961 ccaacgccat ctttcagttt gatcaagccc actttttgca gcagcagcag tgaacgacca
    55021 agattagttg gatcgtttgg cacggcaacc tgcgaaccat cctgcagttc atccagtgat
    55081 ttgattttct tggagtaacc agcaatcgga taaacaaaag tgttgcctac tgcgaccagt
    55141 ttgtagccac gatctttcag ttgctgatca aggtacggtt tatgctggaa ggcgttggcg
    55201 tcgatatcgc ctttgctcaa tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc
    55261 tcaacgtcca ggccatattt gtctttcgca actttctgcg cgacttctgc tacctgctgt
    55321 tcggcaccaa caatcacgcc gactttaatg tggtttggat ctttttcatc ctgaccgcag
    55381 cctaccagtg ccagtgatcc aatcagggct cccactgccg caaaggtttt gaatttgaac
    55441 gccatacctt attccttctt caattattta tgttgtgttg aacgttactt gcgagtgaca
    55501 gcccggacga tgcggtcgcc tgcaaactga attaaataaa ccagaatgac cagcaatacc
    55561 agtaccgtat tcatcaccgt cgcgttgtag ccgatgtagc catactgata gccaatctga
    55621 cctaaaccac cggcaccgac tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg
    55681 gtgatagttg ccgcattcac cagacccggc aacgcttccg gtaacagcac tttacagacg
    55741 atctgcattg gcgtggcacc cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc
    55801 agcagagcgt tctcgaccat acgggcaata aacggtgctg caccaacggt taacggaaca
    55861 attgctgcct gcaaaccaat cgatgtaccg acaataacgc gggtaaacgg aatcatccat
    55921 accagcaaga taatgaacgg gatggaacgg aaaatgttca caatcgcaga aacggtacga
    55981 tacagcttcg cgttagcaat aatttgcccc ggacgcgtga cataaagcag aacgccaacc
    56041 ggcagaccaa tcacaaaccc aaaaaatccg gatacgaagg tcattgccag cgtttcccat
    56101 acgccacgaa ccagcagcca catcatcggc tcagacataa cccagtacct ctacttttac
    56161 atggtgttcc tgcagccagg caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc
    56221 agtcagcatg atgccgaatt taacgccacc ggcgtagtcc atctgcgcgc taataatgtt
    56281 gttgttgacg ttgaaacgac gcgcggtttc agaaagcagt ggggcatcga ccgattgacc
    56341 ggtaaactcc agacgcagca tcggcacgca gtcagtaaat ggctccgttt gcagacgttc
    56401 ctggtaatct tccgggatat ccagatgcag ggtcgactga ataaacttct gcgccagcgg
    56461 cgttttcgga tgcgagaaca cttcacttac cgtgtcctgc tcgatcagtt cgccattgct
    56521 gatgaccgcc acgcaatcac aaatacgctt cacaacgtcc atttcgtggg tgatcaacag
    56581 aatcgtcaaa cccagacggc ggttaatgtc tttcagcagt tcgagaatag aacgtgtcgt
    56641 tgccgggtcc agcgcgctgg tggcttcatc acacagcaat actttgggat tgctggctaa
    56701 tgcacgggca atcgccacac gttgtttctg cccaccggaa agattcgacg ggtagctatc
    56761 atgcttatcg ccaagaccaa ccaatgacag caattccgtc acgcgacgtt tgatctcgtc
    56821 tttcggtgtg ttgtccagct ccagcggcag agccacgttg ccaaaaacag tacgcgaaga
    56881 gagcaggtta aaatgctgga aaatcatacc aatctggcgg cgagctttgg tcaactcgga
    56941 ttctgacagc gtggtcaatt cctggccatc gaccagcacg ctaccctcgg ttgggcgctc
    57001 cagcaagttt acacaacgta taagcgtact cttacccgcg cctgaggcac cgataacgcc
    57061 ataaatttgt ccagctggca catgcaggct gacgttgttc aacgcctgga tggtgcgggt
    57121 gccctggtgg aacactttgg tgatattcga aagttttatc attgattatt tattatcgtc
    57181 attaagttag tcgtagcatc tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat
    57241 gttaaggcat ccagacgtct aaatcaatca ggtttatgcg aagagcactt tcttgcaggt
    57301 cgaaacatgc gatactagcg tcccatgcct tattaaggag ctataaaagg tggcgaagag
    57361 cgtacccgca atttttcttg accgtgatgg caccattaat gtcgatcacg gctatgtcca
    57421 tgagatcgac aactttgaat ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa
    57481 aatgggcttt gcgctggtgg tagtaaccaa ccagtctggc attgctcgcg gtaaatttac
    57541 cgaagcacag tttgaaacgc tgaccgagtg gatggactgg tcgctggcgg accgagatgt
    57601 cgatctggat ggtatctatt attgcccgca tcatccgcag gggagtgttg aagagtttcg
    57661 ccaggtctgc gattgccgca aaccacatcc ggggatgctt ttgtcagcac gcgattattt
    57721 gcatattgat atggccgctt cttatatggt gggcgataaa ttagaagata tgcaggcagc
    57781 ggttgcggcg aacgtgggaa caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga
    57841 agcagaaaac gcggcagatt gggtgttaaa tagcctggca gacctgccgc aagcgataaa
    57901 aaagcagcaa aaaccggcgc aatgattaaa agatgaacgg ttgaaataaa aatgcatttt
    57961 tccgcttgtc ttcctgagcc gactccctat aatgcgcctc catcgacacg gcggatgtga
    58021 atcacttcac acaaacagcc ggttcggttg aagagaaaaa atcctgaaat tcagggttga
    58081 ctctgaaata ggaaagtgtg atatacgcca cctcgcgaca gtgagctgaa agctgcgtcg
    58141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    58201 aacgtcgcaa gacgataaat gaataccaag tctcaagagt gaacacgtaa ttcattacga
    58261 agtttaattc tttgagcatc aaacttttaa attgaagagt ttgatcatgg ctcagattga
    58321 acgctggcgg caggcctaac acatgcaagt cgaacggtaa caggaagcgc ttgctgcttc
    58381 gctgacgagt ggcggacggg tgagtaatgt ctgggaaact gcctgatgga gggggataac
    58441 tactggaaac ggtagctaat accgcataac gtcgcaagac caaagagggg gaccttcggg
    58501 cctcttgcca tcggatgtgc ccagatggga ttagcttgtt ggtggggtaa cggctcacc
//
LOCUS       DBBBSB010000009        58032 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.009, whole genome shotgun sequence.
ACCESSION   DBBBSB010000009 DBBBSB010000000
VERSION     DBBBSB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.009"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(24..278)
                     /locus_tag="ACIUS0_000532"
     CDS             complement(24..278)
                     /locus_tag="ACIUS0_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGB0218432.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACIUS0_000533"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACIUS0_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGB0218433.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACIUS0_000534"
     CDS             1228..2694
                     /locus_tag="ACIUS0_000534"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGB0218434.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACIUS0_000535"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACIUS0_000535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGB0218435.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACIUS0_000536"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACIUS0_000536"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGB0218436.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACIUS0_000537"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACIUS0_000537"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGB0218437.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACIUS0_000538"
     CDS             5709..7265
                     /locus_tag="ACIUS0_000538"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGB0218438.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACIUS0_000539"
     CDS             7347..9161
                     /locus_tag="ACIUS0_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGB0218439.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACIUS0_000540"
     CDS             9162..10256
                     /locus_tag="ACIUS0_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGB0218440.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACIUS0_000541"
     CDS             10256..11281
                     /locus_tag="ACIUS0_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0218441.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACIUS0_000542"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACIUS0_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGB0218442.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACIUS0_000543"
     CDS             complement(12876..13220)
                     /locus_tag="ACIUS0_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGB0218443.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACIUS0_000544"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="ACIUS0_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGB0218444.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACIUS0_000545"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="ACIUS0_000545"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGB0218445.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="ACIUS0_000546"
     CDS             15616..17376
                     /locus_tag="ACIUS0_000546"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGB0218446.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="ACIUS0_000547"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="ACIUS0_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGB0218447.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACIUS0_000548"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="ACIUS0_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGB0218448.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="ACIUS0_000549"
     CDS             19113..19340
                     /locus_tag="ACIUS0_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGB0218449.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="yejM"
                     /locus_tag="ACIUS0_000550"
     CDS             19360..21120
                     /gene="yejM"
                     /locus_tag="ACIUS0_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGB0218450.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="ACIUS0_000551"
     tRNA            21195..21271
                     /locus_tag="ACIUS0_000551"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACIUS0_000552"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="ACIUS0_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGB0218451.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="ACIUS0_000553"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="ACIUS0_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGB0218452.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACIUS0_000554"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="ACIUS0_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGB0218453.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACIUS0_000555"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="ACIUS0_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGB0218454.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACIUS0_000556"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="ACIUS0_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGB0218455.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACIUS0_000557"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="ACIUS0_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGB0218456.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACIUS0_000558"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="ACIUS0_000558"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGB0218457.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACIUS0_000559"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="ACIUS0_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGB0218458.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACIUS0_000560"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="ACIUS0_000560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGB0218459.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACIUS0_000561"
     CDS             complement(30806..31429)
                     /gene="ccmA"
                     /locus_tag="ACIUS0_000561"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGB0218460.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACIUS0_000562"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="ACIUS0_000562"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGB0218461.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACIUS0_000563"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="ACIUS0_000563"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGB0218462.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACIUS0_000564"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="ACIUS0_000564"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGB0218463.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACIUS0_000565"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="ACIUS0_000565"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGB0218464.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACIUS0_000566"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="ACIUS0_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGB0218465.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACIUS0_000567"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="ACIUS0_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGB0218466.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACIUS0_000568"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="ACIUS0_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGB0218467.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /gene="yojO"
                     /locus_tag="ACIUS0_000569"
     CDS             37391..37555
                     /gene="yojO"
                     /locus_tag="ACIUS0_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGB0218468.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37669..38178
                     /gene="eco"
                     /locus_tag="ACIUS0_000570"
     CDS             37669..38178
                     /gene="eco"
                     /locus_tag="ACIUS0_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGB0218469.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACIUS0_000571"
                     /pseudo
     CDS             complement(38300..39924)
                     /gene="mqo"
                     /locus_tag="ACIUS0_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="ACIUS0_000572"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="ACIUS0_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGB0218470.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="ACIUS0_000573"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="ACIUS0_000573"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGB0218471.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="ACIUS0_000574"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="ACIUS0_000574"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGB0218472.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="ACIUS0_000575"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="ACIUS0_000575"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGB0218473.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="ACIUS0_000576"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="ACIUS0_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGB0218474.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="ACIUS0_000577"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="ACIUS0_000577"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGB0218475.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="ACIUS0_000578"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="ACIUS0_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGB0218476.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="ACIUS0_000579"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="ACIUS0_000579"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGB0218477.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="ACIUS0_000580"
     CDS             complement(53338..54114)
                     /locus_tag="ACIUS0_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGB0218478.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="ACIUS0_000581"
     CDS             complement(54119..55768)
                     /locus_tag="ACIUS0_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGB0218479.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..58032)
                     /locus_tag="ACIUS0_000582"
                     /pseudo
     CDS             complement(55769..58032)
                     /locus_tag="ACIUS0_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14198 a        15654 c        14257 g        13923 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca aacaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc atgatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta at
//
LOCUS       DBBBSB010000010        55418 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.010, whole genome shotgun sequence.
ACCESSION   DBBBSB010000010 DBBBSB010000000
VERSION     DBBBSB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.010"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACIUS0_000583"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACIUS0_000583"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGB0218480.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACIUS0_000584"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACIUS0_000584"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGB0218481.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACIUS0_000585"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACIUS0_000585"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGB0218482.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACIUS0_000586"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACIUS0_000586"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGB0218483.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACIUS0_000587"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACIUS0_000587"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGB0218484.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACIUS0_000588"
     CDS             7826..9559
                     /locus_tag="ACIUS0_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGB0218485.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACIUS0_000589"
     CDS             9708..10559
                     /locus_tag="ACIUS0_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0218486.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACIUS0_000590"
     CDS             complement(10546..10887)
                     /locus_tag="ACIUS0_000590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGB0218487.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACIUS0_000591"
     CDS             complement(10889..11767)
                     /locus_tag="ACIUS0_000591"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGB0218488.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACIUS0_000592"
     CDS             complement(11733..14030)
                     /locus_tag="ACIUS0_000592"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGB0218489.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACIUS0_000593"
     CDS             complement(14081..14401)
                     /locus_tag="ACIUS0_000593"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGB0218490.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACIUS0_000594"
     CDS             complement(14416..15495)
                     /locus_tag="ACIUS0_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGB0218491.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000595"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACIUS0_000596"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACIUS0_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACIUS0_000597"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACIUS0_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACIUS0_000598"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACIUS0_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACIUS0_000599"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACIUS0_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGB0218492.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACIUS0_000600"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACIUS0_000600"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGB0218493.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACIUS0_000601"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACIUS0_000601"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGB0218494.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACIUS0_000602"
     CDS             25474..25608
                     /locus_tag="ACIUS0_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218495.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACIUS0_000603"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACIUS0_000603"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGB0218496.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACIUS0_000604"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACIUS0_000604"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGB0218497.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACIUS0_000605"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACIUS0_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGB0218498.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACIUS0_000606"
     CDS             30120..30728
                     /locus_tag="ACIUS0_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGB0218499.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLDKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACIUS0_000607"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACIUS0_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGB0218500.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACIUS0_000608"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACIUS0_000608"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGB0218501.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACIUS0_000609"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACIUS0_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGB0218502.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACIUS0_000610"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACIUS0_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGB0218503.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACIUS0_000611"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACIUS0_000611"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGB0218504.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACIUS0_000612"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACIUS0_000612"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGB0218505.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACIUS0_000613"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACIUS0_000613"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGB0218506.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACIUS0_000614"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACIUS0_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGB0218507.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACIUS0_000615"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACIUS0_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGB0218508.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACIUS0_000616"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACIUS0_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACIUS0_000617"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACIUS0_000617"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGB0218509.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACIUS0_000618"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACIUS0_000618"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGB0218510.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACIUS0_000619"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACIUS0_000619"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGB0218511.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACIUS0_000620"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACIUS0_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGB0218512.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACIUS0_000621"
     CDS             complement(44843..45142)
                     /locus_tag="ACIUS0_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGB0218513.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACIUS0_000622"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACIUS0_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACIUS0_000623"
     CDS             45895..46494
                     /locus_tag="ACIUS0_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGB0218514.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACIUS0_000624"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACIUS0_000624"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGB0218515.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACIUS0_000625"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACIUS0_000625"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGB0218516.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACIUS0_000626"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACIUS0_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGB0218517.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACIUS0_000627"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACIUS0_000627"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGB0218518.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACIUS0_000628"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACIUS0_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGB0218519.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACIUS0_000629"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACIUS0_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGB0218520.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACIUS0_000630"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACIUS0_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGB0218521.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACIUS0_000631"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACIUS0_000631"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGB0218522.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACIUS0_000632"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACIUS0_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGB0218523.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15017 c        14163 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccagac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcgataaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DBBBSB010000011        54498 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.011, whole genome shotgun sequence.
ACCESSION   DBBBSB010000011 DBBBSB010000000
VERSION     DBBBSB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54498
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.011"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            115..747
                     /gene="comR"
                     /locus_tag="ACIUS0_000633"
     CDS             115..747
                     /gene="comR"
                     /locus_tag="ACIUS0_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGB0218524.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(809..1348)
                     /locus_tag="ACIUS0_000634"
     CDS             complement(809..1348)
                     /locus_tag="ACIUS0_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGB0218525.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1575..2879)
                     /gene="ndh"
                     /locus_tag="ACIUS0_000635"
     CDS             complement(1575..2879)
                     /gene="ndh"
                     /locus_tag="ACIUS0_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGB0218526.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3287..3829)
                     /gene="ycfP"
                     /locus_tag="ACIUS0_000636"
     CDS             complement(3287..3829)
                     /gene="ycfP"
                     /locus_tag="ACIUS0_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGB0218527.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3852..4877)
                     /gene="nagZ"
                     /locus_tag="ACIUS0_000637"
     CDS             complement(3852..4877)
                     /gene="nagZ"
                     /locus_tag="ACIUS0_000637"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGB0218528.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(4888..5712)
                     /gene="thiK"
                     /locus_tag="ACIUS0_000638"
     CDS             complement(4888..5712)
                     /gene="thiK"
                     /locus_tag="ACIUS0_000638"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGB0218529.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5693..6334)
                     /gene="lpoB"
                     /locus_tag="ACIUS0_000639"
     CDS             complement(5693..6334)
                     /gene="lpoB"
                     /locus_tag="ACIUS0_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGB0218530.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6349..6726)
                     /locus_tag="ACIUS0_000640"
     CDS             complement(6349..6726)
                     /locus_tag="ACIUS0_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGB0218531.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6729..7088)
                     /gene="hinT"
                     /locus_tag="ACIUS0_000641"
     CDS             complement(6729..7088)
                     /gene="hinT"
                     /locus_tag="ACIUS0_000641"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGB0218532.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7421..9606
                     /gene="fhuE"
                     /locus_tag="ACIUS0_000642"
                     /pseudo
     CDS             7421..9606
                     /gene="fhuE"
                     /locus_tag="ACIUS0_000642"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9666..11099)
                     /gene="ptsG"
                     /locus_tag="ACIUS0_000643"
     CDS             complement(9666..11099)
                     /gene="ptsG"
                     /locus_tag="ACIUS0_000643"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGB0218533.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11394..12191)
                     /locus_tag="ACIUS0_000644"
     CDS             complement(11394..12191)
                     /locus_tag="ACIUS0_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGB0218534.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12202..13206)
                     /gene="holB"
                     /locus_tag="ACIUS0_000645"
     CDS             complement(12202..13206)
                     /gene="holB"
                     /locus_tag="ACIUS0_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGB0218535.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13203..13844)
                     /gene="tmk"
                     /locus_tag="ACIUS0_000646"
     CDS             complement(13203..13844)
                     /gene="tmk"
                     /locus_tag="ACIUS0_000646"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGB0218536.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13834..14856)
                     /gene="yceG"
                     /locus_tag="ACIUS0_000647"
     CDS             complement(13834..14856)
                     /gene="yceG"
                     /locus_tag="ACIUS0_000647"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGB0218537.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14859..15668)
                     /gene="pabC"
                     /locus_tag="ACIUS0_000648"
     CDS             complement(14859..15668)
                     /gene="pabC"
                     /locus_tag="ACIUS0_000648"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGB0218538.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15788..17029)
                     /gene="fabF"
                     /locus_tag="ACIUS0_000649"
     CDS             complement(15788..17029)
                     /gene="fabF"
                     /locus_tag="ACIUS0_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGB0218539.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17116..17352)
                     /gene="acpP"
                     /locus_tag="ACIUS0_000650"
     CDS             complement(17116..17352)
                     /gene="acpP"
                     /locus_tag="ACIUS0_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGB0218540.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17563..18297)
                     /gene="fabG"
                     /locus_tag="ACIUS0_000651"
     CDS             complement(17563..18297)
                     /gene="fabG"
                     /locus_tag="ACIUS0_000651"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGB0218541.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18310..19239)
                     /gene="fabD"
                     /locus_tag="ACIUS0_000652"
     CDS             complement(18310..19239)
                     /gene="fabD"
                     /locus_tag="ACIUS0_000652"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGB0218542.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19255..20208)
                     /gene="fabH"
                     /locus_tag="ACIUS0_000653"
     CDS             complement(19255..20208)
                     /gene="fabH"
                     /locus_tag="ACIUS0_000653"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGB0218543.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20276..21346)
                     /gene="plsX"
                     /locus_tag="ACIUS0_000654"
     CDS             complement(20276..21346)
                     /gene="plsX"
                     /locus_tag="ACIUS0_000654"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGB0218544.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21427..21600)
                     /gene="rpmF"
                     /locus_tag="ACIUS0_000655"
     CDS             complement(21427..21600)
                     /gene="rpmF"
                     /locus_tag="ACIUS0_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGB0218545.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21652..22173)
                     /gene="yceD"
                     /locus_tag="ACIUS0_000656"
     CDS             complement(21652..22173)
                     /gene="yceD"
                     /locus_tag="ACIUS0_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGB0218546.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22372..22956
                     /gene="yceF"
                     /locus_tag="ACIUS0_000657"
     CDS             22372..22956
                     /gene="yceF"
                     /locus_tag="ACIUS0_000657"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGB0218547.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23068..24027)
                     /gene="rluC"
                     /locus_tag="ACIUS0_000658"
     CDS             complement(23068..24027)
                     /gene="rluC"
                     /locus_tag="ACIUS0_000658"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGB0218548.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24600..27791
                     /gene="rne"
                     /locus_tag="ACIUS0_000659"
     CDS             24600..27791
                     /gene="rne"
                     /locus_tag="ACIUS0_000659"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGB0218549.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27988..28930)
                     /gene="flgL"
                     /locus_tag="ACIUS0_000660"
                     /pseudo
     CDS             complement(27988..28930)
                     /gene="flgL"
                     /locus_tag="ACIUS0_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(28942..30584)
                     /gene="flgK"
                     /locus_tag="ACIUS0_000661"
                     /pseudo
     CDS             complement(28942..30584)
                     /gene="flgK"
                     /locus_tag="ACIUS0_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30650..31591)
                     /gene="flgJ"
                     /locus_tag="ACIUS0_000662"
     CDS             complement(30650..31591)
                     /gene="flgJ"
                     /locus_tag="ACIUS0_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGB0218550.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31591..32688)
                     /gene="flgI"
                     /locus_tag="ACIUS0_000663"
     CDS             complement(31591..32688)
                     /gene="flgI"
                     /locus_tag="ACIUS0_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGB0218551.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32700..33398)
                     /gene="flgH"
                     /locus_tag="ACIUS0_000664"
     CDS             complement(32700..33398)
                     /gene="flgH"
                     /locus_tag="ACIUS0_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGB0218552.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33451..34233)
                     /gene="flgG"
                     /locus_tag="ACIUS0_000665"
     CDS             complement(33451..34233)
                     /gene="flgG"
                     /locus_tag="ACIUS0_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGB0218553.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34371..34958)
                     /gene="flgF"
                     /locus_tag="ACIUS0_000666"
                     /pseudo
     CDS             complement(34371..34958)
                     /gene="flgF"
                     /locus_tag="ACIUS0_000666"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(35060..35122)
                     /locus_tag="ACIUS0_000667"
                     /pseudo
     CDS             complement(35060..35122)
                     /locus_tag="ACIUS0_000667"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35142..36281)
                     /locus_tag="ACIUS0_000668"
     CDS             complement(35142..36281)
                     /locus_tag="ACIUS0_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGB0218554.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(36306..37001)
                     /gene="flgD"
                     /locus_tag="ACIUS0_000669"
     CDS             complement(36306..37001)
                     /gene="flgD"
                     /locus_tag="ACIUS0_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGB0218555.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37013..37416)
                     /gene="flgC"
                     /locus_tag="ACIUS0_000670"
                     /pseudo
     CDS             complement(37013..37416)
                     /gene="flgC"
                     /locus_tag="ACIUS0_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(37420..37836)
                     /gene="flgB"
                     /locus_tag="ACIUS0_000671"
     CDS             complement(37420..37836)
                     /gene="flgB"
                     /locus_tag="ACIUS0_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGB0218556.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37991..38650
                     /gene="flgA"
                     /locus_tag="ACIUS0_000672"
     CDS             37991..38650
                     /gene="flgA"
                     /locus_tag="ACIUS0_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGB0218557.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            38726..39019
                     /gene="flgM"
                     /locus_tag="ACIUS0_000673"
     CDS             38726..39019
                     /gene="flgM"
                     /locus_tag="ACIUS0_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGB0218558.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39024..39440
                     /gene="flgN"
                     /locus_tag="ACIUS0_000674"
     CDS             39024..39440
                     /gene="flgN"
                     /locus_tag="ACIUS0_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGB0218559.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39480..41015)
                     /gene="murJ"
                     /locus_tag="ACIUS0_000675"
     CDS             complement(39480..41015)
                     /gene="murJ"
                     /locus_tag="ACIUS0_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGB0218560.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41182..42048)
                     /locus_tag="ACIUS0_000676"
                     /pseudo
     CDS             complement(41182..42048)
                     /locus_tag="ACIUS0_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42050..42697)
                     /locus_tag="ACIUS0_000677"
     CDS             complement(42050..42697)
                     /locus_tag="ACIUS0_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HGB0218561.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42708..43292)
                     /gene="rimJ"
                     /locus_tag="ACIUS0_000678"
     CDS             complement(42708..43292)
                     /gene="rimJ"
                     /locus_tag="ACIUS0_000678"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGB0218562.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43528..44736
                     /gene="mdtH"
                     /locus_tag="ACIUS0_000679"
     CDS             43528..44736
                     /gene="mdtH"
                     /locus_tag="ACIUS0_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGB0218563.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44800..45447
                     /gene="grxB"
                     /locus_tag="ACIUS0_000680"
     CDS             44800..45447
                     /gene="grxB"
                     /locus_tag="ACIUS0_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGB0218564.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            45581..46141
                     /locus_tag="ACIUS0_000681"
     CDS             45581..46141
                     /locus_tag="ACIUS0_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGB0218565.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46247..47293
                     /gene="pyrC"
                     /locus_tag="ACIUS0_000682"
     CDS             46247..47293
                     /gene="pyrC"
                     /locus_tag="ACIUS0_000682"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGB0218566.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47367..47612
                     /gene="dinI"
                     /locus_tag="ACIUS0_000683"
     CDS             47367..47612
                     /gene="dinI"
                     /locus_tag="ACIUS0_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGB0218567.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47902..48156
                     /gene="bssS"
                     /locus_tag="ACIUS0_000684"
     CDS             47902..48156
                     /gene="bssS"
                     /locus_tag="ACIUS0_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGB0218568.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48271..49389
                     /gene="solA"
                     /locus_tag="ACIUS0_000685"
     CDS             48271..49389
                     /gene="solA"
                     /locus_tag="ACIUS0_000685"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGB0218569.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            49437..49550
                     /locus_tag="ACIUS0_000686"
     CDS             49437..49550
                     /locus_tag="ACIUS0_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGB0218570.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49811..50377
                     /locus_tag="ACIUS0_000687"
     CDS             49811..50377
                     /locus_tag="ACIUS0_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGB0218571.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50381..50956
                     /locus_tag="ACIUS0_000688"
     CDS             50381..50956
                     /locus_tag="ACIUS0_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGB0218572.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(50998..52050)
                     /locus_tag="ACIUS0_000689"
     CDS             complement(50998..52050)
                     /locus_tag="ACIUS0_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGB0218573.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52275..53195
                     /gene="lpxL"
                     /locus_tag="ACIUS0_000690"
     CDS             52275..53195
                     /gene="lpxL"
                     /locus_tag="ACIUS0_000690"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGB0218574.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53367..53552
                     /locus_tag="ACIUS0_000691"
                     /pseudo
     CDS             53367..53552
                     /locus_tag="ACIUS0_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53588..>54498
                     /locus_tag="ACIUS0_000692"
     CDS             53588..>54498
                     /locus_tag="ACIUS0_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGB0218575.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT        13320 a        14499 c        13439 g        13240 t
ORIGIN      
        1 ctttaaattt cagtcaaata gaaagcaatt atttttatgg gatctggact ggtgaacgga
       61 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca
      121 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
      181 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
      241 gcggacctcg tcgaagcaac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
      301 aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat
      361 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
      421 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
      481 tgcaccaccc tctccccaga ttcaggagat atagccaata cgttgaaatc acgccatgcg
      541 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
      601 acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg
      661 atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgccgg aacgactttg
      721 cgtttatggc ccgaactggt gaaataagtc gcaaaaaagg cccctcagcc tgagcgaatg
      781 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt
      841 tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat
      901 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
      961 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
     1021 taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta ccgcgaccac
     1081 caccaaactg atgccctatc acgccaccag caacagcgcc gagcaccgac ccggtaatgc
     1141 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
     1201 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggcccc
     1261 gttcaaacac gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc
     1321 cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat
     1381 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatgactg
     1441 gaaatactta aggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
     1501 tatcaggcct accgctcgta ccatttgtcc agggcggata aggcgtttat accacatccg
     1561 ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc
     1621 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
     1681 agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
     1741 ttacccatca ggctaccaac ggtggagaag ttcgacagcg ataccagcga accatgatct
     1801 ttatactgat aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg
     1861 caagtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc
     1921 gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgagtggt ttgcagcgtc
     1981 ggttccacca ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag
     2041 tctggcgctt tgatcccggc tgcccatacc atcagatcag cctcaatata ttcgccatct
     2101 ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg
     2161 ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa
     2221 atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa
     2281 ccgtagctgt gcagttgctt gatcgcgttg tgcaattcag cggagagttc tacacccgtc
     2341 gcgccgccgc cgacaatcgc aatgttcact ttgccatttg cgcccaggtt ggcagagtat
     2401 ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
     2461 aaaatgcagt tctctttgac gcctggcgta ttgaaatcgt tagaggtgct acccagcgcc
     2521 atgaccaggg tgtcataggc gattttacgt tccggaacca acagttcacc tttctcgtcg
     2581 cgtagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccaac
     2641 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
     2701 tcaagcgagc cagtcgccac ttcgtgcagc agtggtttcc acaggtggct gtggttacga
     2761 tcgaccagcg taattttggc ttttttctta cgtcccagct tatgccccag ctgtgttgcc
     2821 atttccagcc caccagcacc gccgccgaca atcacaatct ttttcaatgg cgtagtcaac
     2881 gtgcccccct taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat
     2941 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
     3001 ttggtcatac caaaattgat gttaatcaag ttttgttgct taaaagatag gcaactttac
     3061 cgggggagat caaaaaatag agtttattgt ttattatttg agcaaattcg gaagggtaat
     3121 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagatgcgc agcatcgcat
     3181 caggcattga tgctcgactg tcggatgcgg cgtggacgcc ttatccgact tacagaattc
     3241 ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
     3301 tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt
     3361 cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagtg
     3421 cttcatcatt acgcgacaaa ataaccagac aacgatcgcg attcttctcg cggaagttgg
     3481 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt
     3541 tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa
     3601 tccgttccgc ccagtatccg cccaggccaa cgccgcaaat tagcggacgc tcgtcaacgt
     3661 tcagttgcaa cattttgtcc acttctttaa gcagatgctg catatcatgt ttcggatgcc
     3721 gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct
     3781 cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac
     3841 ataagccagg gttagtgacc tactttctct tcctgccagc gttcatgtaa ctgattcaga
     3901 cgggtgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
     3961 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
     4021 acggcccctt tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatgcc
     4081 tgcccgcgtt cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag
     4141 aaaatcacgc cgtcaaaccc cagttcctga cgcaaaacgg ttttcagcca gtagggagaa
     4201 ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg
     4261 agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct
     4321 tgtggacgtg gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct
     4381 gggaagtgtt tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg
     4441 gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg
     4501 atatgcccga catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg
     4561 ctggccatca gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc
     4621 gcagcaaagg attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt
     4681 ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc
     4741 accagttcac gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc
     4801 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc
     4861 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
     4921 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
     4981 tcctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
     5041 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc
     5101 cacccacacc gccgccagtt ccagcgcgat atcaccatct ccagcatact cccagtcgat
     5161 gagttttaac cctgatgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
     5221 acttaagcgt aaaggtcgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca
     5281 acccacagtc cgccgcgccg gatcgctttg ctgccagtac agttccagta agggcaacag
     5341 cgttattcgc cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc
     5401 gttggtatcc ggcaaatacg tttttaccgc accgggcaga tagtcgacta ccatccagtc
     5461 acggagatat aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata
     5521 ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
     5581 ctggccctga tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac
     5641 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct
     5701 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
     5761 tttgtagggt cggagcgtta acgttgccag aggcgcagga gtacagcacg taatgagcgc
     5821 cgacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
     5881 aacctaactg ttgcttagcc atcgacagtt gctgggcgga aaccagggta aatttcccgt
     5941 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag
     6001 tacggttgtt aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa
     6061 gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg
     6121 caggcggtgc cgttcgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca
     6181 ccgagggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca
     6241 cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg
     6301 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcaccccttt acaaatatag
     6361 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga
     6421 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
     6481 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaatcgt
     6541 tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc
     6601 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
     6661 atgtgaacga cagcccacca gcagtaacaa caccagagac accagcccaa agcatccttt
     6721 tctcatcgtt acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag
     6781 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtattcat gatcagacga
     6841 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
     6901 cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag aatattcgga
     6961 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
     7021 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
     7081 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
     7141 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaatcttaa ctcacgattt
     7201 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
     7261 aatgcgctga aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc
     7321 tcatttgcat ttgcagcaaa attattcgca cactaaatat aaccgctcat caccctcctc
     7381 cggcatgagc ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa
     7441 cagggataat caatatcaag tcatcaccaa accgtcacta cttgccggtt gcatagcact
     7501 ggcactatta ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga
     7561 gggttcagcc acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc
     7621 gggtaccaaa atgcagatga ctcaacatga tattcctcag tcggtcacta ttgttagcca
     7681 gcagcggatg gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg
     7741 gatcagcaaa agtcaggcgg attccgatcg tgctctttat tattcccgcg gattccagat
     7801 cgataactat atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga
     7861 cgcactttct gatatggcac tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact
     7921 catgaccggg acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgacctg
     7981 tcgtgaattt aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt
     8041 ggcggattta caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg
     8101 ctaccagaat aacgactcat ggctggaccg ctacaatagt gaaaagacct tcttctcggg
     8161 cattgtcgat gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg
     8221 cattgatgtt aatagcccaa cctggggcgg tttaccgcgc tggaatactg atggcagcag
     8281 caacagttac gatcgcgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat
     8341 caacaaggtc tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa
     8401 tgccacccac tctgaagtcg aatttgacag caaaatgatg tatgtcgatg cctatgtaaa
     8461 caaagcggat ggtatgctgg ttgggccata cagtaattat ggacctggct ttgatcatgt
     8521 cggcggcacc ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga
     8581 cggtagttat gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa
     8641 acaaaacaat cgttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac
     8701 aactttaatg gcaatttccc acaagccgac tggtcatcac agagcctggc gcaggacgat
     8761 accacacata tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
     8821 ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag
     8881 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
     8941 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
     9001 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
     9061 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
     9121 ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc
     9181 agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt
     9241 ggcgcaacgc gctatcttgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
     9301 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc
     9361 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc
     9421 ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg
     9481 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
     9541 atggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
     9601 ttctgaataa cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt
     9661 acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta
     9721 ccgaaaatag cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
     9781 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
     9841 gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt
     9901 tcgctggtac ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga
     9961 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg
    10021 acgatcggta acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga
    10081 ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa tacagattgg gaatgccagg
    10141 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg
    10201 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    10261 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    10321 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    10381 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc
    10441 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccaggca acgttcgatg
    10501 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    10561 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    10621 gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc
    10681 agcttaatac ggtagaaacg gttaaacatg tacgctgcaa tcgcaccgga gataatccct
    10741 ccgagtacgc cagtatccgc caagtgttta gaggcgattt cttcagcagg taaatgcagt
    10801 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc
    10861 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt
    10921 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    10981 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg tagtacggat
    11041 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    11101 attgagagtg ctcctgagta tgggtgcttt ttttacgttc tcacgcgtgg caagggggga
    11161 gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat
    11221 tcagtgccaa tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca
    11281 cattttgaca aaacatatgt cacaatattt caaacgcggt tattcaccgc ccaattttcg
    11341 gcggtgaact ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg
    11401 atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc
    11461 tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga
    11521 accatcgcag gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca
    11581 gtttccacca gtaatcgatc cagggggaca taacgcgcag catcgcgcag ttgctccgca
    11641 ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc
    11701 gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct
    11761 tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg
    11821 ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt
    11881 tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc
    11941 gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca
    12001 caagaaaata cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt
    12061 aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg
    12121 tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg
    12181 actaaaaaca tgatgtctct cttaaagatg aggaaccggt aacacaacgc ccggttgcag
    12241 gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac
    12301 agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcacgaggg
    12361 agaaagatgg tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcacctgcgc
    12421 agcgccatga tggcgtttta gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc
    12481 cggagcttgt tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct
    12541 atatgccaac gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc
    12601 cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga
    12661 cattgtcact tcgcgtgaaa gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta
    12721 atgtaaccga caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag
    12781 gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc
    12841 gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg
    12901 ctcattcagc ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt
    12961 ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg
    13021 acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc
    13081 gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc
    13141 tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca
    13201 tctcatgcgt ccaactcctt cacccagtgg gtcacggtag tgcggattgc atccatcacg
    13261 gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc
    13321 agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc
    13381 tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt
    13441 aagtctgggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca
    13501 ataccacgtc ctccgccctg atacgcctga gtggaaagat cgtggcgatc gccaatcacc
    13561 caggtgccgt tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca
    13621 taaaacatca gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc
    13681 agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc
    13741 atgtcgcgga tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg
    13801 cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac
    13861 tttcagataa tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc
    13921 acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt
    13981 cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt
    14041 gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc
    14101 catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat
    14161 aaataccgag gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga
    14221 tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca
    14281 ggcgctatcg accgctttca ccattttctt gtgaactcgc ttgagtaacg cgacatcggt
    14341 ggtattggcg gtatacatca aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt
    14401 ttccagttca agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat
    14461 atacggagcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg
    14521 cagcgggaac tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat
    14581 ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag
    14641 cagccattga aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag
    14701 cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt
    14761 gctgtcggca agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac
    14821 cagcaataac aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt
    14881 ggggctaaat attcatataa cgttgctgac gaaaaggtga catcgccaca ggcacgtacg
    14941 ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcctcc
    15001 agcgaggctt gcacttcgac aagctgataa gaggattgtg ccagtaaacg gatacagaat
    15061 tgtcgcataa tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacattgccc
    15121 ttccgccaga acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc
    15181 agcgcctcat cagcgtttgt ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga
    15241 ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc
    15301 aacgtcatcc cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga
    15361 atccgcgttg ctggtccgct gttcaatgtg ctgtaccctc gcccgccact accgcgactg
    15421 atcacgactt tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca
    15481 agctgaggcc agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgcagg
    15541 atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca
    15601 tcaccaaact gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt
    15661 aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac
    15721 aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa
    15781 tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt
    15841 tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat
    15901 cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg
    15961 ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag
    16021 atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct
    16081 gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct
    16141 gactcgcttc aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc
    16201 cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt
    16261 cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac
    16321 cggcaccatc gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt
    16381 gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg
    16441 gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa
    16501 tacgcgcagc atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc
    16561 ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg
    16621 acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt
    16681 cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt
    16741 tctcttccgt tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat
    16801 attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa
    16861 aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta
    16921 ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga
    16981 caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc
    17041 aaggagggaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    17101 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    17161 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    17221 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    17281 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    17341 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    17401 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    17461 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    17521 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    17581 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    17641 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    17701 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    17761 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    17821 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    17881 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    17941 ttttgacaga cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc
    18001 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    18061 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    18121 attcaacatc agacctttgc cgttggcacc taaataatca ctgatcgcct gagcgccatt
    18181 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    18241 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    18301 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    18361 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc
    18421 atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg
    18481 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    18541 atcaacgtta ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac
    18601 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa
    18661 cggcagcgcg cgttttgcgc cagcagcttt acaggcagcg ccagcacgct caaccgcttc
    18721 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    18781 accttctgca gcttcttcac acgctttcgc aataggcgca tcatccagac cgatgattgc
    18841 cgccatagcg cccgtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac
    18901 cagacgcacc gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc
    18961 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata
    19021 cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    19081 agctgacccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    19141 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    19201 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    19261 acgaaccagc gcagagcccc aagtgaatcc accgccaaag gcttcaagca gaaccaactg
    19321 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    19381 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt
    19441 tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    19501 agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac
    19561 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    19621 gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt
    19681 ggaaatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    19741 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    19801 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    19861 ggtgaaacct gcacaggctg ctgcaacgtc aaatgccggg caacctttaa tgtccaacat
    19921 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    19981 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    20041 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    20101 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    20161 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    20221 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    20281 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    20341 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    20401 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    20461 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    20521 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    20581 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat
    20641 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    20701 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    20761 tactgtcaag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    20821 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    20881 tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    20941 gtggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    21001 ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    21061 ctttcaccag ctccagggcc atacgcattg aactcccacg gctggcgcgg atagcttgcg
    21121 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    21181 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    21241 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    21301 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    21361 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    21421 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    21481 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    21541 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    21601 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    21661 ctttaagctg gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc
    21721 aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    21781 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    21841 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    21901 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    21961 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    22021 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    22081 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    22141 atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    22201 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    22261 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    22321 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    22381 cttattttag cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct
    22441 tttgaatgtg cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    22501 ttggtgcttc gactggcaca agaaaaatcg caatctctgg cgtcacgtta tccggatcat
    22561 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    22621 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    22681 actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt
    22741 gacgtccatt tccgtcacct aagcgaggcg gagattgata attacgtgcg taaggagcat
    22801 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt
    22861 ttagagggac gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    22921 ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg
    22981 tcgcatccgg caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc
    23041 gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    23101 cttcaaacct tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    23161 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    23221 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    23281 ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt
    23341 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    23401 gacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct
    23461 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt
    23521 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    23581 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    23641 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    23701 ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc
    23761 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    23821 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    23881 cacccgcact tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    23941 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    24001 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    24061 gccactcatt tttctatgct tatttttagt tttgcacctt attacttcac tacgtgatca
    24121 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    24181 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    24241 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    24301 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    24361 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    24421 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    24481 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    24541 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    24601 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    24661 ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    24721 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    24781 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    24841 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    24901 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    24961 gtctggcggg tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc
    25021 gccgtatcga aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    25081 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc
    25141 aatgggattt aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    25201 gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt
    25261 atttacgtca ggatatcggc gaaatcctta tcgataaccc gaaagtcctc gaactggcac
    25321 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    25381 gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc
    25441 gtgaagtccg tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaacag
    25501 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta
    25561 acaccaacct cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    25621 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    25681 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    25741 gctttggcct gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc
    25801 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc
    25861 tctctattct gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    25921 ccattgttcc tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg
    25981 cgattgaaac gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa
    26041 ccccgcacta ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca
    26101 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    26161 gtaagcgtcc tgaacaacct gcactggcaa cctttgccat gccggatgta ccgccagcgc
    26221 caaccccagc tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa
    26281 caccagcagc tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc
    26341 tgttcagcgg tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga
    26401 aaccggaacg tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg
    26461 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    26521 gtaatcgtcg ccaggcacag cagcaaactg ccgagacgcg tgagagccgt cagcaggctg
    26581 aggtaacgga aaaagcacgt accaccgacg agcagcaagt gccgcgtcgt gaacgtagcc
    26641 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    26701 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    26761 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    26821 tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc
    26881 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    26941 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    27001 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    27061 caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    27121 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg
    27181 aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    27241 aacctgttgt tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac
    27301 cggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    27361 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg
    27421 cagaacacgc agaaccgatg gttgaaccgc aggaagagac ggcagatgca gatattgaag
    27481 aagttgccga aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg
    27541 cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga
    27601 tcaccgttga gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg
    27661 ttccggaggc accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag
    27721 gtgccgcagg tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac
    27781 ctgttgagta ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat
    27841 ttcatgtttg aatcaacata ttaaaattca tcaaatggca tccttgctaa ccaacaatgc
    27901 aaaataggca aaaaaaatgc ccgccccggc gatcccagag cgggcagaca aaacataccc
    27961 agtttcatga tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg
    28021 catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga
    28081 aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc
    28141 gcgatcgcta cctaaggaat ccagcgactc cagtttgttc agctgcgtgc ctaattccgc
    28201 gcgaacggta aggacattat tcagcgagtt tttcagtcca cggttggttt tatccaatgc
    28261 cgcagcggcg atttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat
    28321 ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac
    28381 cgcgttactg gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg
    28441 cgaagcatcg acctgttgct taatactttc tgttccgcca acataatcac cgttagcctc
    28501 actaaacggc gcagtctctg ttttatatgt agcgcccatt accgtcagtg gtgttcgcca
    28561 gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt
    28621 cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg
    28681 tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca
    28741 gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa
    28801 tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca
    28861 tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac
    28921 tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc
    28981 cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta
    29041 ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac
    29101 attaccttgc gtggtgcgac tggttttcaa cgtcgcggtt ttattaccga tatcactcac
    29161 taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg
    29221 cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc
    29281 catcgcgatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac
    29341 tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc
    29401 atcaaatgcc actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag
    29461 gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac
    29521 ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag
    29581 aaccgcgggc ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc
    29641 ggctttgtgt tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt
    29701 acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt
    29761 cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt
    29821 acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac
    29881 cagtgagtaa ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct
    29941 acgccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta
    30001 ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt
    30061 tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg
    30121 tcctggtcgc gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc
    30181 ccaatcagcg cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg
    30241 gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg
    30301 attttcgaca tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt
    30361 aactggttgg taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg
    30421 ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg
    30481 gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg
    30541 gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag
    30601 ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat
    30661 tatcaatgtt catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga
    30721 tcatgttggt gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga
    30781 cctgcgcccc ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg
    30841 ttaacagccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg
    30901 cttttacttt cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc
    30961 ctttccagtt gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc
    31021 ggcggatttg ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat
    31081 gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg
    31141 ctctactgtc acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct
    31201 gcgaaagcgt ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg
    31261 ctggcgtgga ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg
    31321 caagccccag acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg
    31381 tatacagacg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc
    31441 ttttcaacat catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg
    31501 ccgccggatc ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg
    31561 cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca
    31621 tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc
    31681 attgagcgca cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc
    31741 gccgctctgg cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa
    31801 cggtgtatct ggctggctga cattggcctg acgattaact gttactgaga gatttccctg
    31861 cgctaccgcg cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga
    31921 gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc
    31981 aaggaaacgg acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc
    32041 taacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc
    32101 catgctgaaa tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct
    32161 gggcaattca cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac
    32221 ctgaacactg ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc
    32281 cagcgcatac acctgactgt caacgccctt aagcggagtc atcaacaacg tacctccacg
    32341 caagcttttg gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc
    32401 aaacggagga agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt
    32461 gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa
    32521 cggcgtctgg gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat
    32581 cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc
    32641 cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact
    32701 tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta
    32761 atgtagccat tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta
    32821 ttgctgccgc tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc
    32881 tgattaatgg caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg
    32941 tcaaccgtca ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg
    33001 ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc
    33061 aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca
    33121 gaggagcttt tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata
    33181 ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac
    33241 tggaaaatag aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc
    33301 ccctgcacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag
    33361 ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag
    33421 taaaccggtg aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg
    33481 atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt
    33541 gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc
    33601 cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta
    33661 gaggttttcg ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg
    33721 cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc
    33781 gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc
    33841 agccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga
    33901 tgagccatct ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct
    33961 gttattggtc tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac
    34021 ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg
    34081 ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt
    34141 agtactgacg tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag
    34201 gcccgttttg gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca
    34261 agcagagcag aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg
    34321 ggcgagggaa tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc
    34381 gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc
    34441 atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta
    34501 ctgccttcca gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct
    34561 cgcgtggcct gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg
    34621 ctgcccgtgg ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg
    34681 cccggattga gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc
    34741 acagcaattg gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg
    34801 ggatcaacct gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg
    34861 gccagccatc ctgctgcaac gcaacgtcca gcgggcgaga ggtgtaatcc atcttgccgg
    34921 gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc
    34981 cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat
    35041 tggccagatt actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg
    35101 cagtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc
    35161 gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg
    35221 gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg
    35281 ttggtcagag tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat
    35341 tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc
    35401 gccagtacaa tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta
    35461 ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt
    35521 gccacaatgt tgttagcgcc ggttgcgata ttattcgcca tcgcaccatc cactaatgtg
    35581 ccattagcat taaattccag tgttgtcgct gtcttcgcaa tgctgtttgg atcactgcta
    35641 tcctgggtgt agacctgcca gttattatcc ccggtcttca caaaatagac gctcatgtca
    35701 tgagcattac cctgactgtc gaaaacagtc accgaacctt ttttgttata gctatccgca
    35761 ttgctggcgc tgaatggcgt aacagtagga agcggatcac tggaattcag gttgatctgc
    35821 atcgacgccg tggtggtagt tttcgctgcc atcagggtat tcgggatcga aatattggtc
    35881 ggattcgccc cttgctgaat agtcggcggc gtaccggtta ccgggtaacc cgtcagctgt
    35941 aaaccttgcg tattcaacag gttacggttt tcatccagct taaattgtcc gttacggctg
    36001 tagaacaccg aaccgttgct gtctaccaga cggaaaaaac cgttctggct gatagcaacg
    36061 tccagacctc gcccggtgtt ggtggtcgtg ccatcggtaa agtcctgagt gataccggca
    36121 acttttaccc ccagtcccac tttcgaaccg gcaaacatat cggcaaaaga ggccgtgcct
    36181 gatttaaagc cgtaggtggc ggagttggcg atattgttgc caataacatc gaggttggtg
    36241 gcggcagcgt ttaatccgct aaccgcttga gaaaaggcca tgactgactc ctgaaatgtg
    36301 aaggcttaaa ttatctgccg tacttcgtcg agggtggtgg tgccgtaagt gccgagatcc
    36361 agcgtattac cgctgttgcc gcggatcaca ccctgcacca gagcaaactg cagcggctgg
    36421 gcaaccagtt gtgtaccacc gttactggcg ctaatcgcta cattgtaaga accgttcggc
    36481 gcagtgctgc catcagtcaa cgaaccgtcc caggtgaaac tgtgaactcc ggcggtcagt
    36541 tcaccaatat caatggtgcg cacaaccgcg ccatttttat cggtgatggt ggccgtaact
    36601 ttgtctgccg cctgttgcag ctcaacacca aacggcgtgg tcgtggtcac agccccttct
    36661 tcactgccgg ttcccgcaag aacagttgtg ccggggatca tcacgccgtg accgatcagg
    36721 ttactggcct gtaacgactg gctgttatca atctgtccgg aaatagatcc gagcgtggta
    36781 ttgagttttt caatcccgct gaccgtgctg atttgtgcca attgcgacgt cagctcgttg
    36841 ttttccattg gattggtcgg gtcctggttt ttcagctgcg ccaccagcaa agtcagaaaa
    36901 ctgctttgta aatctgcggc gttgctgccc gtgagcgaac tactgctggt ggtactgacg
    36961 ccggtatttg tcggatcggt ggtggttacc gcaatggaca tagctttctc ctttattgac
    37021 cgagcgtaag ggttttcagc atcatgcttt tcaccgtgtt gagcacttca acattggcct
    37081 gatagctgcg tgacgccgac atggtgttaa ccatctcccc gacgacatca acgttcggca
    37141 tttttatgta gccctttgca tctgccagcg gattacctgg ttcataaacc agtttgtccg
    37201 gggcctgact ttcttaacat cggcaacctt tacgccgcct gtcgcagcac ctggtgcagc
    37261 gttaacctgg aataccacct gttttgcccg atatggctgt ccatcaggac cggtcacgct
    37321 atcagcattt gccagattac tggccgccac gttcaggcgc tgggactggg cagttaacgc
    37381 cgacccggcg atatcaaaaa tattcagcag tgccatccgt taatttccgc tctgtaaaac
    37441 gttcatcatg cctttgattt gcccgctcaa cgcgctaagg ctcatctggt attgcaggct
    37501 gttatcggca aactgggtgc gttcgcgatc catatcgacg gtattaccgt caagcgaagg
    37561 ctggtccgga atacggtatt gcagttctgc ggtaggaggc gtcagcgcct gcgccgggat
    37621 gtgctgcgtt gaggtcatcg tcagtgcaac cacactggtt gcatcccgtc cacgttgcat
    37681 gactttttta agttcactgg caaaatcgat atcgcgcgcc tgataaccag gggtatcggc
    37741 attggcgatg tttgctgcca gcacttcctg acgctgggcg cgcagattga gcgcctcttg
    37801 ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct cctccgcagg tatcaaaatt
    37861 ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc ggaataaacg caaaatgggt
    37921 cgctatttat gccgttgatg gtcattgcgg acaggtacaa ttcacgttgt agaaatggct
    37981 gggggcgaaa atgctgacaa taaaacgtag cgtggcgatc atcgcgatac tgttcagtcc
    38041 gttaagtacg gcgagcaatc tcacatcgca attgcacaac ttttttagcg cccaactcgc
    38101 gggggtaagt gatgaggttc gtgtttctat tcgtacagcg cccaatctac taccgccatg
    38161 cgagcagcca ttgctttcga tgagcaataa ttcccgcctg tggggcaatg tgaatgtgtt
    38221 ggcacgctgc ggtaacgaca aacgatattt acaggttaat gtgcaggcca caggaaatta
    38281 tgtggttgcc gcgatgccta ttgtgcgggg aggaaagctg gaagctggca atgtcaaact
    38341 gaaacgtgga cgcctggata ccctgccacc gcgtacggtg ctggatatca atcaacttgt
    38401 tgatgccgtt agcctgcgcg atctatcacc cgatcaacct atccagttaa cccagtttcg
    38461 ccaggcatgg cgggtaaaag cgggacaacg cgtcaatgtg atcgccagcg gtgatgggtt
    38521 tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc gtcgcacaga atgcgcgggt
    38581 gcgcatggta tcggaacagg tagtcagcgg cgttgttgat gcagatggga atattcttat
    38641 aaacctgtaa gctgttaaag attacccgtc ccttgccgat aaataagcaa cacatgataa
    38701 aagcgccctc aatgaggaat aaaccatgag tattgatcgc acttcgcctc tgaagcctgt
    38761 aagcaccgtt caaccgcgcg aaaccaccga cgcgccggta acgaacagcc gggcggcaaa
    38821 aacaaccgcc tccaccagca ccagtgtgac gttaagcgac gcgcaagcaa aactgatgca
    38881 acccggcagc agtgatatca atcttgaacg tgtcgaagcg ttaaaactgg cgattcgtaa
    38941 cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg ctgatcaacg aagcgcagca
    39001 agacttgcag agtaactgat cgtatgacac gtcttgcaga gatcctcgac cagatgtccg
    39061 ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca gcaacatctc tctatggggc
    39121 agatcaacgg cagccagttg caatggatta cagaacaaaa aagctcactg ctggcgacgc
    39181 tggattacct cgaacagtta cgcaagaaag aacccaatac agcaaatagc gttgatatta
    39241 gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact acgccaaatg aatcaacata
    39301 acggctggtt actggaagga cagattgagc gcaatcaaca ggcgctggaa atgttgaaac
    39361 cgcatcagga accgacgcta tatggggcga acggtcagac ctcaacaccc catcgcggca
    39421 gtaaaaagat ttcgatctga atattatctc cggcctgcac tgcaggccgg aatgcattgt
    39481 tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca gtaccgccag tgcagcgaag
    39541 tacgcggcaa tccccgccag cacgaccgcc attaaacgca gtaagcgcca gggcatggta
    39601 cccaatgacc actccggcat gatatgtaac atacctaaaa gcacgccaga catcaccagt
    39661 accgccacca ccagacgcaa cagaaacgcc atccagccgg gttgcggagt aaagattttc
    39721 tgcttacgca actgccagta aagcagcgaa gcattcagac acgccgccag accaatagaa
    39781 agtgacagcc cggcatgttt caacggacca ataaacgcca ggttcatcaa ttgcgttaaa
    39841 atcagcgtaa cgatggcaat tttcactggc gttttaatgt cctggcggga ataaaagcca
    39901 ggagccaaca ctttcactac aatcaggcca atcaaaccca ccgagtaggc aattaacgcc
    39961 cgctgggtca tcagcgcatc aaacgcggta aatttaccgt actggaacag cgaaacggtt
    40021 aacggacctg aaagaatgcc caacgcaacc gcactcggca gtgccaacag gaaacaaaga
    40081 cgcaaccccc agtccatcaa acggttgtat tcatcatgat tgccactggc aaaacttttc
    40141 gacagcgacg gcagcaaaat ggtgccaagc gccacgccca gcacaccgga gggaaactcc
    40201 attaagcggt cggcgtaata catccaagac accgaaccgg aagcaagaaa cgaggcaaaa
    40261 atggtgttga tgattaagga gatctggctc acagagacgc caaggatcgc cggtcccatc
    40321 tgtttcacca cgcgcattgc tccagcatcg tggaagttaa tgcgcggcag gaccagcatg
    40381 ccgatcttct tcaggtgcgg tagctgatac accagctgca gaatgccgcc gaccgtaaca
    40441 gcccacgcca gcgccagcac cggtgggtta aagtacggtg cggcaaacag cgcgaaacca
    40501 atcatgctga tgttaagcag tgttggcgca aacgccggaa tcgagaagcg gttccacgta
    40561 ttcagaatcg ctcccaccag cgacgccagg gagatcagca agatataggg aaaggtaatc
    40621 tttagtagct ggctggtcag ggcaaatttg tcagctgtgt cagcgaagcc tggcgcggtc
    40681 accatgatca cccacggtgc ggcgagcatg ccagcgaccg tcacaaccgc cagcgcaagt
    40741 gtcagcaggc cagaaacata agagacaaag acccgcgtgg cgtcttcacc ctgcttactt
    40801 ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg ccccttcggc aaagatacgg
    40861 cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt cggttgccat ccctgcgcca
    40921 aagattctgg cgacaattgc gtctcgtgcg aagccaagca cacgcgaaaa catggtcatc
    40981 gagctgacgg cggccagcga ttttaataaa ttcatcggtg ttctaatccg gacccacggg
    41041 caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt ctacctagat tatggtgaat
    41101 tgctaccgcc agatgttaca gggtttattc actcatcgca tcgcgccaga tcttgtcaac
    41161 gatacgttgc gccagcactg cctattcgcc ggcggtttgc ggaactgtct gattttacac
    41221 gcattcaatg aagtgccgcg cacagccgac aaatccacgt tgctcaagag tgctctgcca
    41281 accaggaatc ggtttattca ctacaccctg cccgcgctcc tcacgccatt cgcgcatatc
    41341 cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg gtttcacgct gacttccggc
    41401 acggcgatgc atacaagtgg tgatctgcaa aggaccggcc gaaaaatggt gctcggcaaa
    41461 cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca tccagagagg ctttgccgcc
    41521 cgacaaccac agcgcggtat ccaccacatg cagataatca tccagcaacg tgaaataaag
    41581 atcgtgtggc ccgacgctat tgctacgatg tttatccatt cttagcgagg ctgcggtggc
    41641 gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga cggttaaaac cgaccatcaa
    41701 cgtcagtttt ttccgcgccg ccagttccac cagcctttca gcatcgcgca gattttctgc
    41761 cagcggttta tcgacacaga catgtacccc cgcattgagt aacgtactga ccacgtcaaa
    41821 gtggctggcg gtgctggagt gcacaaaaac cgcatcacaa ctggcggcaa ggctggataa
    41881 cgaatcggca taaggaatgc gccagctgtc acaaattggc agggctttgg cgcgcgtagg
    41941 cgaccaggct ccttgtaacg tccagtcaga cgctgccgcc agcaccggta accacgcttt
    42001 ttgcgcaatg ccgcctaatc ccactacgcc gatacgtaat tttttcactt taatctccca
    42061 gatgggccag caacgaatca agacgttgtt tcagttctgc cacttcgatt tccagggctt
    42121 cgacgcgggt ctgtaaatca ccgtcaacca tgttcgacat agccgttacc gccggctgat
    42181 cttcaacctc accactgaaa agatgcatgt agcggctttc gcgtttaccc ggttcgcggg
    42241 ccagacgcac cacaaaagga ccatcttcgc gattcgccag ttgttccagc gtcgactcca
    42301 cttccgccat atcgctgaat tcatacattc gcgcggcgcg gctgcgcagt tcacccggcg
    42361 tctgggcacc acgcaataac aacgtggtga ttaacgccac ttctgcggcg ctcagtttca
    42421 gatcgccaaa ttctgaatta caaaaacgtt gctcatattt ggtgacccga ttaccaaaac
    42481 cgctcactgt gcgtagataa tgacgtttga ccagattatc cagctgttcc tgcacttcgg
    42541 attcactcag attcatgacc ggttcacggt tcgttttctg attacaggcc gtgactacac
    42601 cattgactga gagcgggtat tgctccggcg tggtcacctg cttttccagc aagcaaccaa
    42661 ttacgcgagc ttccagggca gtaagttgat atttcatgag attctcctta gcggccgggc
    42721 gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc gccattgtcc atcaatcaac
    42781 agatagtctt tcgcatagcc ttctttttca aaacccagtc gcgccagtaa atcaccgctg
    42841 cgtttattgt gcggcatata attagccata atgcgatgaa tatgttgggt gcgctgcata
    42901 taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt tgccctgcca tttttgccca
    42961 atcgaataac cgagatagca ggcatgaaaa gagccgcgaa caacattgga aaaattggca
    43021 acgccaataa tctctttttc gtccgggtcg aataagccaa agtagaaagc tgaaccttgt
    43081 ttatgaaatt cgttaatcat ccctagcctg gcctgccagc ctgatggata acagtggctt
    43141 tcgtcgcgca ctggctccca gggcttgagg aaatggcgat tctctgcgta ataatccgcc
    43201 agacgccagg catcacgatc atgcaccaga cgcacgacca gtcggtctgt ggttaagcgc
    43261 acttttggca cgttactgcg atagccaaac attctatacc tactccttcc cgtaacgtct
    43321 ttaatacgct gtaagtatta ctatacctgc gcaaccagac cctgtgaaaa gtccaaaatg
    43381 ccattttctg cattattcac atatttgata aaaactctct atcaaagcga cgttttgata
    43441 aaaaaatact gtccccgaag tgatgggaaa agtcttagcg gcaatgcaga atagaaagtg
    43501 ctcatctttt atttcccggg aggggaaatg tcccgcgtat cgcaggcgag gaacctgggt
    43561 aaatatttcc tgctcatcga taatatgctg gtcgtgctgg ggttctttgt tgtcttcccg
    43621 ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg ccgtcatggt cggtattgct
    43681 ctcggtctac gccagtttat tcagcaaggt ctgggtattt tcggcggcgc aattgccgac
    43741 cgctttggtg ccaaaccgat gattgttacc ggtatgctga tgcgcgccgc cggattcgcc
    43801 acaatgggta tcgcccacga accgtggcta ttgtggtttt catgcctgct ctcgggactc
    43861 ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg tgaaactaat ccgtccacaa
    43921 caacgtggtc gttttttctc gctgttgatg atgcaggaca gtgccagtgc ggtcattggc
    43981 gcattgttgg ggagctggct gttgcaatac gactttcgcc tggtctgcgc cacaggggcc
    44041 gttttgtttg tgctatgtgc ggcgttcaat gcgtggttgt taccggcatg gaaactctcc
    44101 accgtacgca cgcccgttcg cgaaggcatg acccgcgtga tgcgtgacaa gcgttttgtc
    44161 acctatgtcc tgacgctggc gggttactac atgctggctg tacaagtgat gctgatgctg
    44221 ccaattatgg tcaacgacgt ggctggcgcg ccctctgccg ttaaatggat gtatgccatt
    44281 gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc gctggagtga aaagcatttt
    44341 cgtctggaac accggttgat ggctgggctg ttgataatgt cattaagcat gatgccggtg
    44401 ggcatggtca gcggcctaca acaacttttc accctgattt gtctgtttta tatcgggtcg
    44461 atcattgccg agcctgcgcg tgaaacctta agtgcttcgc tggcggacgc aagagctcgc
    44521 ggcagctata tggggtttag ccgtctgggt ctggcgattg gcggcgctat tggttatatc
    44581 ggtggtggct ggctgtttga cctgggcaaa tcggcgcacc agccagagct tccgtggatg
    44641 atgctgggaa ttattggcat cttcactttc cttgcgctgg gttggcagtt tagtcagaaa
    44701 cgcgccgcgc gtcgtttgct ggaacgcgac gcctgatttg atactccccc ctgactcctc
    44761 catactgaaa gtgaactgac agccaaatgg aggagtcacg tgaagctata catttacgat
    44821 cactgccctt actgcctcaa agcccgcatg attttcggcc tgaaaaatat ccccgtcgaa
    44881 ttacatgttc tgctcaacga cgacgcagaa acacccaccc ggatggtcgg tcaaaaacag
    44941 gttcccattc tgcaaaaaga tgacagccgc tatatgccag aaagcatgga tatcgttcac
    45001 tatgtcgata aactcgacgg caaaccgtta ctgaccggca aacgttcccc tgccattgaa
    45061 gagtggctgc gcaaggtcaa tggctacgtc aacaaactgc tgttgccgcg ttttgccaaa
    45121 tcggcatttg atgagttttc tactcccgcc gcgcgcaaat atttcgtcga caagaaagag
    45181 gccagcgcgg gtaattttgc cgacctgctg gcccactctg acggtctgat taagaatatc
    45241 agcgatgatt tgcgtgcgct ggacaaactg atcgtcaaac cgaacgccgt gaatggcgaa
    45301 ctttcggaag atgatattca gctatttccg ctactgcgta atctgacgct ggtagccgga
    45361 atcaactggc caagccgcgt tgctaattac cgcgataata tggcgaaaca gacacaaatc
    45421 aatttgttat catcaatggc gatttaatcc tgacccgccg cggcagcctg tattcgcggg
    45481 ttgccgccgt tttcagtttt ttctgctggc gagtttatcg ctggtgacac atgctgcgtt
    45541 gtgtcaggat attgacgcag atttcatgtc gaggactacc atgaacaagt ttttatttgc
    45601 cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat caactcaccc aatacaccat
    45661 caccgaacaa gaaattaacc agtcgcttgc gaaacataat aatttctcaa aagatatcgg
    45721 tttacccggc gtggctgacg cccatattgt tctgacaaac ttgaccagcc aaattggtcg
    45781 cgaagagccg aataaggtta ccctaaccgg agacgccaat ctggacatga actccctgtt
    45841 cggtagtcag aaagcgacca tgaaactgaa gctgaaagcg ctgcccgtgt ttgataaaga
    45901 aaaaggtgcg atcttcctga aagagatgga agtggtcgat gcgacggtac aaccggaaaa
    45961 aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac caggcattac gcaattactt
    46021 taaccagcaa cctgcttacg tcctgcgcga agatggcagt cagggcgaag caatggcgaa
    46081 aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt gtcattccgt ttacggatta
    46141 atgacgaggg cgcattcgcg ccctttattt ttcgtgcaaa ggaaaacgtt tccgcttatc
    46201 ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag agattaatga ctgcaccatc
    46261 ccaggtatta aagatccgcc gcccagacga ctggcacctt cacctccgcg atggcgacat
    46321 gttaaaaact gtcgtgccgt ataccagcga aatttatgga cgggctatcg taatgcccaa
    46381 tctggctccg cccgtgacca cagttgaggc tgccgtggcg tatcgccaac gtattcttga
    46441 cgccgtacct gccgggcacg atttcacccc attgatgacc tgttatttaa cagattcgct
    46501 ggatcctaat gagctggagc gcggatttaa tgaagccgtg ttcaccgctg caaaacttta
    46561 cccggcaaac gcaaccacta actccagcca cggcgtgacg tcaattgacg caatcatgcc
    46621 ggtacttgag cgcatggaaa aaatcggtat gccgctactg gtgcatggtg aagtgacaca
    46681 tgcagatatc gacatttttg atcgtgaagc gcgctttata gaaagcgtga tggaacctct
    46741 gcgccagcgc ctgactgcgc tgaaagtcgt ttttgagcac atcaccacca aagatgctgc
    46801 cgactatgtc cgtgacggaa atgaacggct ggctgccacc atcactccgc agcatctgat
    46861 gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg cacctgtatt gtttacccat
    46921 cctcaaacgc aatattcacc aacaggcatt gcgtgaactg gtcgccagcg gttttaatcg
    46981 agtattcctc ggtacggatt ctgcgccaca tgcacgtcat cgcaaagaga gcagttgcgg
    47041 ctgcgcgggc tgcttcaacg ccccaaccgc gctgggcagt tacgctaccg tctttgaaga
    47101 gatgaatgct ctgcagcact ttgaagcatt ctgttctgta aacggcccgc agttctatgg
    47161 cttgccggtc aacgacacat tcatcgaact ggtacgtgaa gagcaacagg ttgctgaaag
    47221 catcgcactg actgatgaca ccctggtgcc attcctcgcc ggggaaacgg tacgctggtc
    47281 cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg ttattttacc tgtataaata
    47341 accagtatat tcaacagggg gctattatgc gaattgaagt caccatagcg aaaacttctc
    47401 cattgccagc tggggctatt gacgccctgg ctggcgaact ttcccgccgt attcagtatg
    47461 cgtttcctga taatgaaggc cacgtatcgg tacgttatgc cgcagcgaat aatttatcgg
    47521 ttattggcgc aacaaaagaa gataaacagc gcattagcga aattctccag gaaacgtggg
    47581 aaagcgccga tgactggttt gtcagcgaat aatatgcagt gatttttgtt tttgccggat
    47641 cgccccggct tttttatttt acttacgaat tttgagtaca aaatttctac acttcctcaa
    47701 aataattgca caatatgcta aaaaaatatg aatgacatgc tgttttattg ttcatataaa
    47761 tcttaaaagc agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta
    47821 ttggtatact gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt
    47881 aacacgaatt agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc
    47941 gtagggtggg acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac
    48001 cagaccccaa ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc
    48061 actgatgttg ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc
    48121 ggtgatttcc cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa
    48181 ggcacctcac cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc
    48241 tgcttaaacc atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc
    48301 ggttccgtag gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg
    48361 accgacgccc atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt
    48421 cgccatgctt atggtgaagg cgaaaagtat gtcccgttgg tcctccgcgc gcaaacgctg
    48481 tgggatgaac tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt
    48541 aacctcggcc cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg
    48601 caactcaacg ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc
    48661 gtcccggaca actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg
    48721 gcgattaaaa cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc
    48781 ccggtcaccg caattcgtca tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag
    48841 tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag
    48901 ctgcctgtcc agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc
    48961 gtgaagaata aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt
    49021 tttccggcag aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat
    49081 tcagcggatg aacgtgttcc gtttgcggaa gtggccagtg atgggtcaga agccttcccg
    49141 ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat
    49201 gataattcgc ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg
    49261 ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct
    49321 gccgattttg cgcaagacca aaaaagcgat tttgatttga cgccattcag gctttcccgc
    49381 ttccaataat catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc
    49441 gtcggctgtt gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc
    49501 tctggggatt gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac
    49561 tgacataaac tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat
    49621 tattcctgct tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga
    49681 agattctttc attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt
    49741 atttttgtgt tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtatt
    49801 ggatgcactt atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt
    49861 ccattggcta agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac
    49921 gctcagttat tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat
    49981 tttgttaatg atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg
    50041 tccgctgccg agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt
    50101 gtgcctattg ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa
    50161 accgatcagc gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca
    50221 ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtctgt
    50281 tatgcacgga tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg
    50341 catgctggga aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg
    50401 tttaaccttc gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat
    50461 tgacaaagaa ggtcagcacg cctttgttaa cttccgcata cagcatcttg gctatagctg
    50521 gttatatggc acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccggc
    50581 tgccgataaa gtgaatgtga caattaacac caccagcgtc gatactaacc acgccgaacg
    50641 cgataaacat cttcgcagtg cggattttct caataccaca aaatatccac aggcaacatt
    50701 cacctccacc agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct
    50761 gaatggcgta accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc
    50821 atggggcggt aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa
    50881 tatcaagaca gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg
    50941 cgtacagcag aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta
    51001 ttctgttgga tcaggaatac ccagtgttgt attcagacgt ccacgagact tattaaagat
    51061 cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct
    51121 ttcttcgcag caaatctcac tacaacaacc tttgtatttt tccgcgcata ctggacactg
    51181 aataaacagc aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcgcc
    51241 gcactggtgg caatgcgcga taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa
    51301 aacaaaattt ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta
    51361 ttcaataatt ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc
    51421 actggccttt tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg
    51481 tgcctgcatc atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc
    51541 gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag
    51601 tgcatcggga tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt
    51661 gctggcatca aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat
    51721 gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc
    51781 taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac
    51841 gctgatttgc gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc
    51901 ggtaaacagc tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt
    51961 gtaaaacgaa atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc
    52021 gttggaaatg cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta
    52081 aaaatccgcg gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca
    52141 tttgcgcatt aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc
    52201 acaaattttg gttgcgggcg aaaaaatgcg acaatacata caattgcccg tataggttga
    52261 aaaacaggat tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt
    52321 attggttaac ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccga
    52381 ttatctaccg cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg
    52441 caaaaattgt gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc
    52501 gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggtatgg
    52561 cgtggttctg gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca
    52621 ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac
    52681 tggagctggg cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga
    52741 acgataatcc actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga
    52801 tgctcgaccg caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct
    52861 ggtacgcacc agatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg
    52921 ttgagcaggc tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc
    52981 tggtgccctt cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc
    53041 caccagagtg ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag
    53101 tggtcgaaaa atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta
    53161 aaacacgccc ggaaggcgtt ccttcacgct attaaatctc ccatgccgga tgcttcagaa
    53221 tggcatccgg cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg
    53281 cgccccctgg aagtcgggcg cataattagt gtgtttatct tttcttctta tgttcaccgc
    53341 gcctggcgca ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact
    53401 ggaaacgaaa cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc
    53461 tggtaatgcc cttcttaccc ctctacgttg agcagcttgg tgttaccggc cactccgccc
    53521 tgaatatgtg gtccggtatt gtcttcagta taatgggttc ctaaacacat cattttaggt
    53581 attgaatatg agacgggcaa tcagctacat cagattttca agtgaacgac aactgaaagg
    53641 ggactctgta cgccgacaat ctaagctggt tactgactgg cttgataaga accctgagtt
    53701 ttacttggat tcatccttga gctttaaaga tttggggaaa agtgctttta gtggtaagca
    53761 cctgaaaggt ggtctaggtg actttttaac cgctatcgag aaagggcttg ttaaagctgg
    53821 tgatacgcta ctaatcgaga gtttagaccg tctgtcacgt caggatattg atattgcttc
    53881 tgagctatta cgccgtatct taagggctgg tgtagatgta gtcacactga gcgatggaga
    53941 acattacaca cgagaaagtt taaaagaccc gttagccctg attaaaagca ttctgataat
    54001 gcagcgggcg catgaggaaa gtttgagaaa gtctgagcgt gtccaagctg catggaaccg
    54061 taaaaaagaa ctcatctccg aaggtatcaa ggtatctagg cgttgtcctg catggttaag
    54121 gctgaatgat gaccgtcgaa cattcacaat cattcctgat aaagtggaag ttgttaaacg
    54181 agcatttgac ctgagattac agggtttaag cttttgggct atcaccagaa ctttaaacga
    54241 tgaaggtcac ttgtcattga atcaatacac acccaaacaa aaggggtggt ctgatactgc
    54301 tgtaaagaaa ctgctcagaa atcgtgctgt gattggatgt ttcacacctg ctggacgtga
    54361 agaagttcag ggttactacc ccgcaataat cagtgagtca ctattctatc gagtacagca
    54421 actgaacaca gggcaatatg gtagggcttc tgtatcaagt aatccgctat cagtcaactt
    54481 gttcaggggt atcatcaa
//
LOCUS       DBBBSB010000012        54468 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.012, whole genome shotgun sequence.
ACCESSION   DBBBSB010000012 DBBBSB010000000
VERSION     DBBBSB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54468
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.012"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="ACIUS0_000693"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="ACIUS0_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGB0218576.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="ACIUS0_000694"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="ACIUS0_000694"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGB0218577.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="ACIUS0_000695"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="ACIUS0_000695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGB0218578.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACIUS0_000696"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="ACIUS0_000696"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGB0218579.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACIUS0_000697"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="ACIUS0_000697"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGB0218580.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="ACIUS0_000698"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="ACIUS0_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGB0218581.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="ACIUS0_000699"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="ACIUS0_000699"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGB0218582.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="ACIUS0_000700"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="ACIUS0_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="ACIUS0_000701"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="ACIUS0_000701"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGB0218583.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="ACIUS0_000702"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="ACIUS0_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGB0218584.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACIUS0_000703"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="ACIUS0_000703"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGB0218585.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="ACIUS0_000704"
     CDS             complement(13538..13933)
                     /locus_tag="ACIUS0_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGB0218586.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACIUS0_000705"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="ACIUS0_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGB0218587.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="ACIUS0_000706"
     CDS             complement(15220..15447)
                     /locus_tag="ACIUS0_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGB0218588.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="ACIUS0_000707"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="ACIUS0_000707"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGB0218589.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="ACIUS0_000708"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="ACIUS0_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGB0218590.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACIUS0_000709"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="ACIUS0_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGB0218591.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECCV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACIUS0_000710"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="ACIUS0_000710"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGB0218592.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="ACIUS0_000711"
     tRNA            19688..19764
                     /locus_tag="ACIUS0_000711"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACIUS0_000712"
     CDS             complement(19839..21092)
                     /gene="amiC"
                     /locus_tag="ACIUS0_000712"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGB0218593.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21324..22655
                     /gene="argA"
                     /locus_tag="ACIUS0_000713"
     CDS             21324..22655
                     /gene="argA"
                     /locus_tag="ACIUS0_000713"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGB0218594.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACIUS0_000714"
     CDS             complement(22717..24543)
                     /gene="recD"
                     /locus_tag="ACIUS0_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGB0218595.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHDTTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACIUS0_000715"
     CDS             complement(24543..28085)
                     /gene="recB"
                     /locus_tag="ACIUS0_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGB0218596.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACIUS0_000716"
     CDS             complement(28078..30966)
                     /gene="ptrA"
                     /locus_tag="ACIUS0_000716"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGB0218597.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACIUS0_000717"
     CDS             complement(31142..34510)
                     /gene="recC"
                     /locus_tag="ACIUS0_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGB0218598.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34523..34873)
                     /locus_tag="ACIUS0_000718"
     CDS             complement(34523..34873)
                     /locus_tag="ACIUS0_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGB0218599.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34858..35265)
                     /locus_tag="ACIUS0_000719"
     CDS             complement(34858..35265)
                     /locus_tag="ACIUS0_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGB0218600.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35262..35825)
                     /locus_tag="ACIUS0_000720"
     CDS             complement(35262..35825)
                     /locus_tag="ACIUS0_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGB0218601.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35816..36286)
                     /locus_tag="ACIUS0_000721"
                     /pseudo
     CDS             complement(35816..36286)
                     /locus_tag="ACIUS0_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACIUS0_000722"
     CDS             complement(36470..37264)
                     /gene="thyA"
                     /locus_tag="ACIUS0_000722"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGB0218602.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACIUS0_000723"
     CDS             complement(37271..38146)
                     /gene="lgt"
                     /locus_tag="ACIUS0_000723"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGB0218603.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000724"
     CDS             complement(38297..40543)
                     /gene="ptsP"
                     /locus_tag="ACIUS0_000724"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGB0218604.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACIUS0_000725"
     CDS             complement(40556..41086)
                     /gene="rppH"
                     /locus_tag="ACIUS0_000725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGB0218605.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACIUS0_000726"
     CDS             complement(41439..41585)
                     /gene="ygdT"
                     /locus_tag="ACIUS0_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGB0218606.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            41771..42460
                     /gene="mutH"
                     /locus_tag="ACIUS0_000727"
     CDS             41771..42460
                     /gene="mutH"
                     /locus_tag="ACIUS0_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGB0218607.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42529..43242
                     /locus_tag="ACIUS0_000728"
     CDS             42529..43242
                     /locus_tag="ACIUS0_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGB0218608.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43380..43598
                     /gene="ygdR"
                     /locus_tag="ACIUS0_000729"
     CDS             43380..43598
                     /gene="ygdR"
                     /locus_tag="ACIUS0_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGB0218609.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43706..44746
                     /locus_tag="ACIUS0_000730"
     CDS             43706..44746
                     /locus_tag="ACIUS0_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGB0218610.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACIUS0_000731"
     CDS             complement(44779..45969)
                     /gene="lplT"
                     /locus_tag="ACIUS0_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGB0218611.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     LRRH"
     gene            complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACIUS0_000732"
     CDS             complement(45962..48121)
                     /gene="aas"
                     /locus_tag="ACIUS0_000732"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGB0218612.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48707..49738
                     /gene="galR"
                     /locus_tag="ACIUS0_000733"
     CDS             48707..49738
                     /gene="galR"
                     /locus_tag="ACIUS0_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGB0218613.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACIUS0_000734"
     CDS             complement(49745..51007)
                     /gene="lysA"
                     /locus_tag="ACIUS0_000734"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGB0218614.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51129..52064
                     /gene="lysR"
                     /locus_tag="ACIUS0_000735"
     CDS             51129..52064
                     /gene="lysR"
                     /locus_tag="ACIUS0_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGB0218615.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACIUS0_000736"
     CDS             complement(52051..52743)
                     /gene="ygeA"
                     /locus_tag="ACIUS0_000736"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGB0218616.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACIUS0_000737"
     CDS             complement(52872..54290)
                     /gene="araE"
                     /locus_tag="ACIUS0_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGB0218617.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13320 a        14734 c        13423 g        12991 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacagcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg gcaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcaa gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct
    19801 gatctcccat aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt
    19861 gccccatcgg caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg
    19921 aaagtcgccg ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc
    19981 agaatggagg gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt
    20041 ttatgcagct tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg
    20101 tcagcaatag tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
    20161 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
    20221 gcagtactgg ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga
    20281 ctggtaaagg cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt
    20341 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
    20401 ttgccctctt cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt
    20461 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
    20521 ataatgacaa tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc
    20581 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
    20641 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
    20701 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
    20761 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
    20821 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
    20881 acgcgttcag ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
    20941 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca
    21001 gccaggctga cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga
    21061 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
    21121 tgtgtaacaa gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc
    21181 atctaataaa cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc
    21241 tcgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa
    21301 ataatcatgc aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat
    21361 tccgccattc ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg
    21421 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
    21481 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
    21541 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
    21601 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
    21661 caatgagtct caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt
    21721 ttattattgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
    21781 tccggcggat tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa
    21841 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
    21901 ccactcaact agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
    21961 gcgttactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
    22021 gggtagaagc ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg
    22081 gcgcagtgaa agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag
    22141 atggcgcgct gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
    22201 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
    22261 ttcgcccgct ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg
    22321 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
    22381 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
    22441 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
    22501 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
    22561 ttaccccggt ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc
    22621 gtaaatccaa agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca
    22681 ggcgtaacac tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc
    22741 cgccagacca ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc
    22801 ggcatacagc gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt
    22861 tactaccggc gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg
    22921 cgatttatgt accgtcatcg cccacgtagt gtcgtgctct ggcaagcgac tcggttgcac
    22981 agacttaata ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag
    23041 cgcaataccg atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac
    23101 cggtcggcct tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat
    23161 aaactgttca attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca
    23221 caaaagctgg tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag
    23281 cagatccaga taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc
    23341 gccgctctgt aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac
    23401 tgccgtttta tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc
    23461 gaaacgatag cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc
    23521 ggttcctgcc ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt
    23581 aaagcccgcg ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc
    23641 cagttgatca cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag
    23701 tctcgacatc ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg
    23761 cagcgggtta ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg
    23821 gtgcaaagtg ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg
    23881 tcgcaaagcc ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc
    23941 cagacggata cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc
    24001 cacggtggtc gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag
    24061 cgccactgcc gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc
    24121 cagggtttgc gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa
    24181 gcgcgctacc gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag
    24241 gatcatcggc gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc
    24301 ccaattttgt agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc
    24361 gttattttcc agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa
    24421 cagtgccgcc gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg
    24481 cacatccagc gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt
    24541 cattacgcct cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg
    24601 gcgttgggtc gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg
    24661 aacagataga taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc
    24721 agataacgat gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc
    24781 atcgctgctg ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat
    24841 ttatagtcga gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt
    24901 aacatgccgc gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac
    24961 tggcgaatta gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac
    25021 tccatctcca cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg
    25081 agaggtgcct ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca
    25141 aagccgccga gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg
    25201 aggtcttcaa acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc
    25261 ggtgttaacg tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt
    25321 ggcatcagat cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca
    25381 cgccagttat cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca
    25441 tcattaacct gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca
    25501 tcgcataacg cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc
    25561 agcaaacgcc cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg
    25621 cgcaccagcg gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa
    25681 agcaaacgca gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca
    25741 gcattaagat ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg
    25801 acgcggaaat tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta
    25861 tggatcgtga caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag
    25921 gcattactgt ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt
    25981 tccagctgcg ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc
    26041 cgctcaccgc ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc
    26101 cgcagcatcg gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg
    26161 actaccgcat cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc
    26221 cccatcattg acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc
    26281 gcctgcaaca accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc
    26341 gaaaggtaaa cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc
    26401 tggcggctgc gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg
    26461 ttcatcagca acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca
    26521 catacctgcg ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc
    26581 cacattttca tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc
    26641 ccggctgatt tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg
    26701 ctgaaaagct tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa
    26761 gtgtagtggg catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg
    26821 aatgcatata tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg
    26881 tgccagatac ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc
    26941 attgccaccg ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc
    27001 agcgcggaat cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt
    27061 tcacgcgcta ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc
    27121 gacaatggtt ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc
    27181 cccccggcct tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc
    27241 aactgataac tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc
    27301 tgattgctac ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt
    27361 tcacccactg cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg
    27421 tggcgggaag ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct
    27481 tgcagataac gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg
    27541 acctgggcta tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc
    27601 tggtagcgta gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca
    27661 aaggcattca ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca
    27721 tccatctgcc gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct
    27781 tccagcaggc gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac
    27841 tcgtggatat tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg
    27901 accaccagca gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca
    27961 agtaacaggc gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct
    28021 tcaatcaggc gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca
    28081 ctcattcttt tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac
    28141 tttccagcct tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga
    28201 cagaatagcc atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg
    28261 cggcgtcagc agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc
    28321 gcgatcgaaa tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag
    28381 catctgggta attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa
    28441 ttttgcctct gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt
    28501 atcattgctt tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc
    28561 aaacacggca tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac
    28621 gatctgcccc aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac
    28681 aaataccgct gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg
    28741 ttttttatcg accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg
    28801 tttttgcaca tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat
    28861 aaactctggt cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt
    28921 aatggagggc aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat
    28981 ctgcgcgggc ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg
    29041 gttataccag gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc
    29101 ctcgagcaat gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat
    29161 aaggccgttg ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa
    29221 ctgatcaagc gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc
    29281 gctgtccatg gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc
    29341 aaaataacgg cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg
    29401 atggtcgtat ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag
    29461 ctctggcaaa gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc
    29521 gctgatttta tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc
    29581 tttcgggctg atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt
    29641 tactgcttta gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg
    29701 aacgcgaatc atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg
    29761 ataacggaag tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc
    29821 tttttcacgt aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc
    29881 caggccttta tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat
    29941 aggatcggag ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag
    30001 tgtacctggg ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa
    30061 cttcgctgag ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg
    30121 gacgtaatga atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg
    30181 ttttttgctc tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc
    30241 cggcagcggt ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc
    30301 gtggaaatct ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag
    30361 gttaccacca gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg
    30421 tgccatgcgc atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg
    30481 ctcacgttcg gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc
    30541 taccgcaccg ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc
    30601 agtgctggca ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg
    30661 cgggtacttt ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta
    30721 cgcctcggga tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc
    30781 ctgcggatca gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg
    30841 gcggttatct ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc
    30901 ctgacttaat ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg
    30961 gggcattcac ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca
    31021 aatcatctgc cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc
    31081 agtccgtgac tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca
    31141 ctcatgactg attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg
    31201 cctccattgt ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt
    31261 cgccacgcac catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca
    31321 acgtggaatc gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg
    31381 cgccgccact ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa
    31441 tcagttgtga gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac
    31501 cgtctttgcg tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt
    31561 gttccagcca aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca
    31621 ggccatccgg ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat
    31681 caatttccat actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca
    31741 tctcctggca ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg
    31801 ccgcccggaa gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata
    31861 actgctgatt gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga
    31921 tttcgctgtc ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg
    31981 gatgtgccca gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca
    32041 gcggctgaac aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac
    32101 gagcatagct ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat
    32161 ggaggcaagt aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat
    32221 cgcccggtag ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg
    32281 ggaaacgttc actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct
    32341 gcgcggaaat taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc
    32401 cacgcttcgg tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa
    32461 cgccgtcgtt cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca
    32521 gagtacaaat gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac
    32581 gctgtgccag ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta
    32641 aaccttcggc gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt
    32701 ccgcatccgg caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta
    32761 gcggacgctc ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca
    32821 gatgccccac cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc
    32881 actcgccctg cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc
    32941 aggtgtgttg tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc
    33001 aacgaatgcc ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt
    33061 caaaccgcgc cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc
    33121 gactgtcagg cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg
    33181 cacgacggtc ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg
    33241 cctgaataaa cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag
    33301 taagtgtcgg ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac
    33361 gctgcgggct atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt
    33421 tgttatcgct acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt
    33481 ccagaatgtc agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt
    33541 ccagctcctg gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc
    33601 cccatgaggc cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc
    33661 cggcattttc gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct
    33721 gacgagtcag cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc
    33781 aggggttggt aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga
    33841 gataaacagg cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg
    33901 ggcaggtcgt cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc
    33961 agcgcggttg tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc
    34021 aggcctgtgc ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt
    34081 ccggacgata gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca
    34141 gttttcgctt gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct
    34201 ccagcaattg cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc
    34261 tctctttggg gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca
    34321 gcggaaaatc aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact
    34381 gcgccatacc ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt
    34441 cgcgttcgac aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga
    34501 cccttaacat agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc
    34561 cttctctgcc cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg
    34621 tctgcattcg gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct
    34681 gccaaccatg ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg
    34741 ttcgctggat acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca
    34801 gcaccatcag caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca
    34861 gggaagctga cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga
    34921 aaaaacaatt tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc
    34981 ggcaatcaat aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc
    35041 agcgtactgc gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc
    35101 cagcgccgac tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga
    35161 ggcaaaactg cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag
    35221 catcaggacc agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc
    35281 acgctatagc tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta
    35341 accgtcaaca ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata
    35401 atgatggcat ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg
    35461 cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc
    35521 ggttcgcgat cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc
    35581 tgtccgacaa tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg
    35641 agatgcttcg cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga
    35701 gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat
    35761 acgctactga tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca
    35821 ggcatccttc tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca
    35881 gccaccactc gcccgttgag tttttgaagc gaatatgcac ggcccatgcg gtattgcgca
    35941 ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca
    36001 caaagaccaa tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc
    36061 accataacgt cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat
    36121 cttcacgtaa atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg
    36181 actgctgcca gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca
    36241 tcgcgaccag cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga
    36301 gcgaggagaa aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt
    36361 tacgccgttg caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg
    36421 taaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca
    36481 ccggcgcttt aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt
    36541 agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc
    36601 ggcttaattg cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg
    36661 tccagacaaa atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg
    36721 cgtagctggc aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct
    36781 ggcaagagag tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg
    36841 ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt
    36901 cgtttttcag ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg
    36961 gccaggcgcg ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt
    37021 cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga
    37081 accacagcag ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga
    37141 atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg
    37201 tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt
    37261 tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga
    37321 tcacacccgc gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc
    37381 aggcaccggt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc
    37441 cgtagccaat caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca
    37501 ggttgaggat aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt
    37561 ggcgcggcag cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca
    37621 gcaaaatatc ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa
    37681 cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg
    37741 gaatgagtgg tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga
    37801 agataatcat caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc
    37861 agacacggaa cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat
    37921 aaccaatacg tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt
    37981 catttttggt ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa
    38041 aaatgaaccc caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga
    38101 aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt
    38161 tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct
    38221 ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat
    38281 atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca
    38341 taaaggctgc aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg
    38401 caagattttc tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag
    38461 aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg
    38521 gatcgcccgc catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg
    38581 cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc
    38641 gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga
    38701 tgaaatcgac cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca
    38761 tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg
    38821 cacgttcaat caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca
    38881 gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga
    38941 agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt
    39001 cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga
    39061 cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg
    39121 acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa
    39181 tacagctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta
    39241 tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa
    39301 gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga
    39361 ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct
    39421 gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg
    39481 agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa
    39541 gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc
    39601 cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag
    39661 cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga
    39721 ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac
    39781 gcagccgggt gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt
    39841 gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg
    39901 ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt
    39961 ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg
    40021 gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag
    40081 aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat
    40141 actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa
    40201 ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc
    40261 tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga
    40321 cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg
    40381 tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg
    40441 tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg
    40501 cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac
    40561 ctcttttacg tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca
    40621 tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca
    40681 cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc
    40741 tggtttgcat attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt
    40801 tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc
    40861 gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt
    40921 caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg
    40981 gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac
    41041 aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa
    41101 ataaagcttt atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg
    41161 cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca
    41221 gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact
    41281 acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa
    41341 atattcatta ttagtcaatg cattacatgt aatttgagta ataaaaaaga gtaattcgtg
    41401 acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc
    41461 acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc
    41521 aaggctgtaa atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat
    41581 aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt
    41641 tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca
    41701 tgtggaaaaa gttacactgc gaatattcgg catataattg ctgtttgttt tttaaccaag
    41761 gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt
    41821 gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg
    41881 gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat
    41941 ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga
    42001 acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt
    42061 tgcccggtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct
    42121 caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg
    42181 cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga
    42241 ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca
    42301 cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat
    42361 tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt
    42421 caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc
    42481 tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca
    42541 tggataaccg atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt
    42601 cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa
    42661 cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg
    42721 gcatcaatct cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa
    42781 atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc
    42841 aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca
    42901 ttcctcggcg ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg
    42961 attaccgctg tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc
    43021 gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg
    43081 gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt
    43141 ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt
    43201 gaaagcctca acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct
    43261 gcgggagggc gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat
    43321 atataacgtc ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga
    43381 tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt
    43441 ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg
    43501 aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa
    43561 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
    43621 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
    43681 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
    43741 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
    43801 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
    43861 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
    43921 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
    43981 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
    44041 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
    44101 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
    44161 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
    44221 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
    44281 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
    44341 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
    44401 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
    44461 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
    44521 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
    44581 gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
    44641 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
    44701 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
    44761 taaaaccggc gttaaatatt aatgacggcg cagccagatc cacagcgccg ttattgccag
    44821 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
    44881 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
    44941 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
    45001 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
    45061 ctcgtgttgc agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca
    45121 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
    45181 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
    45241 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
    45301 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
    45361 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat
    45421 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag
    45481 gacatgccag tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc
    45541 agaagcttcc attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag
    45601 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    45661 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    45721 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag
    45781 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    45841 cgctttcagt aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa
    45901 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    45961 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    46021 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    46081 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    46141 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    46201 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    46261 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    46321 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    46381 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    46441 gccctttcag ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat
    46501 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    46561 aaacgacagg cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat
    46621 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    46681 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    46741 cgaacaacac ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg
    46801 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    46861 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    46921 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    46981 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    47041 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    47101 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    47161 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    47221 ccgccgtaat agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa
    47281 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    47341 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    47401 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    47461 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    47521 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    47581 ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag
    47641 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    47701 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    47761 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    47821 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    47881 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    47941 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    48001 caataaaaga gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt
    48061 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    48121 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    48181 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    48241 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    48301 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    48361 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
    48421 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat
    48481 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
    48541 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
    48601 tgcgtttggc tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt
    48661 ccgtaacact gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa
    48721 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
    48781 acccaaagcc agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta
    48841 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat
    48901 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
    48961 ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg
    49021 tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat
    49081 gatcccagat gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
    49141 ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg
    49201 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
    49261 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
    49321 tggtattccg gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
    49381 acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa
    49441 cgattcaatg gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg
    49501 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
    49561 gaccaccgtg cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc
    49621 actggcggat aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg
    49681 tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
    49741 gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc
    49801 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg
    49861 acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt
    49921 ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
    49981 cacataacgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
    50041 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
    50101 tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct
    50161 gagtaattaa tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca
    50221 cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat
    50281 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
    50341 aaatagcctg taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct
    50401 gctccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca
    50461 gatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
    50521 tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa
    50581 cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag
    50641 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
    50701 catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg
    50761 cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc
    50821 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca
    50881 gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
    50941 cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat
    51001 gtggcataac aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat
    51061 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
    51121 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
    51181 ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg
    51241 aactggcgcg ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat
    51301 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
    51361 tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
    51421 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
    51481 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
    51541 ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa
    51601 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc
    51661 tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt
    51721 cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac
    51781 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
    51841 taggtatttc ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg
    51901 tgcggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc
    51961 cgtcatcagc gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg
    52021 tcacttctct tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
    52081 ggcatggatc gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa
    52141 accaatttct gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat
    52201 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
    52261 aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa
    52321 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag
    52381 cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa
    52441 gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca
    52501 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc
    52561 cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct
    52621 atgtagcagc atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
    52681 cagacgatag taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt
    52741 cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc
    52801 ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc
    52861 ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg
    52921 atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac
    52981 gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt
    53041 gtcaggaagg ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta
    53101 ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc
    53161 gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac
    53221 aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg
    53281 cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc
    53341 gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc
    53401 atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg
    53461 atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca
    53521 cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt
    53581 tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac
    53641 acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg
    53701 aagaccacca gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc
    53761 cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg
    53821 accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa
    53881 agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg
    53941 gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg
    54001 atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac
    54061 agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg
    54121 gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga
    54181 ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta
    54241 tcacgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc
    54301 cagcagagag taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg
    54361 aaacagcata aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat
    54421 tttaaaatta agttgaatta ttgagattat tattaacctc ctaatttt
//
LOCUS       DBBBSB010000013        52809 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.013, whole genome shotgun sequence.
ACCESSION   DBBBSB010000013 DBBBSB010000000
VERSION     DBBBSB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.013"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..358
                     /locus_tag="ACIUS0_000738"
     CDS             <1..358
                     /locus_tag="ACIUS0_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGB0218618.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
     gene            complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="ACIUS0_000739"
     CDS             complement(396..1295)
                     /gene="nhaR"
                     /locus_tag="ACIUS0_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGB0218619.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="ACIUS0_000740"
     CDS             complement(1361..2527)
                     /gene="nhaA"
                     /locus_tag="ACIUS0_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGB0218620.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3113..3265
                     /gene="mokC"
                     /locus_tag="ACIUS0_000741"
     CDS             3113..3265
                     /gene="mokC"
                     /locus_tag="ACIUS0_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGB0218621.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="ACIUS0_000742"
     CDS             complement(3369..4499)
                     /gene="dnaJ"
                     /locus_tag="ACIUS0_000742"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGB0218622.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="ACIUS0_000743"
     CDS             complement(4588..6504)
                     /gene="dnaK"
                     /locus_tag="ACIUS0_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGB0218623.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6881..7285
                     /locus_tag="ACIUS0_000744"
     CDS             6881..7285
                     /locus_tag="ACIUS0_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGB0218624.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7311..8024
                     /gene="msyB"
                     /locus_tag="ACIUS0_000745"
     CDS             7311..8024
                     /gene="msyB"
                     /locus_tag="ACIUS0_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGB0218625.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8173..8739
                     /gene="satP"
                     /locus_tag="ACIUS0_000746"
     CDS             8173..8739
                     /gene="satP"
                     /locus_tag="ACIUS0_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGB0218626.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8774..9361)
                     /gene="mog"
                     /locus_tag="ACIUS0_000747"
     CDS             complement(8774..9361)
                     /gene="mog"
                     /locus_tag="ACIUS0_000747"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGB0218627.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9476..10429)
                     /gene="tal"
                     /locus_tag="ACIUS0_000748"
     CDS             complement(9476..10429)
                     /gene="tal"
                     /locus_tag="ACIUS0_000748"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGB0218628.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10708..12138
                     /locus_tag="ACIUS0_000749"
                     /pseudo
     CDS             10708..12138
                     /locus_tag="ACIUS0_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12208..12984
                     /gene="yaaA"
                     /locus_tag="ACIUS0_000750"
     CDS             12208..12984
                     /gene="yaaA"
                     /locus_tag="ACIUS0_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGB0218629.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13137..13433)
                     /locus_tag="ACIUS0_000751"
     CDS             complement(13137..13433)
                     /locus_tag="ACIUS0_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGB0218630.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="ACIUS0_000752"
     CDS             complement(13647..14933)
                     /gene="thrC"
                     /locus_tag="ACIUS0_000752"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGB0218631.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="ACIUS0_000753"
     CDS             complement(14934..15866)
                     /gene="thrB"
                     /locus_tag="ACIUS0_000753"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGB0218632.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="ACIUS0_000754"
     CDS             complement(15868..18330)
                     /gene="thrA"
                     /locus_tag="ACIUS0_000754"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGB0218633.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18356..18475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="ACIUS0_000755"
     CDS             complement(18411..18476)
                     /gene="thrL"
                     /locus_tag="ACIUS0_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGB0218634.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18690..19376)
                     /locus_tag="ACIUS0_000756"
     CDS             complement(18690..19376)
                     /locus_tag="ACIUS0_000756"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGB0218635.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19776..19916)
                     /gene="yjjY"
                     /locus_tag="ACIUS0_000757"
     CDS             complement(19776..19916)
                     /gene="yjjY"
                     /locus_tag="ACIUS0_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGB0218636.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20012..20728
                     /gene="arcA"
                     /locus_tag="ACIUS0_000758"
     CDS             20012..20728
                     /gene="arcA"
                     /locus_tag="ACIUS0_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGB0218637.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20787..22139)
                     /gene="creD"
                     /locus_tag="ACIUS0_000759"
     CDS             complement(20787..22139)
                     /gene="creD"
                     /locus_tag="ACIUS0_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGB0218638.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22197..23621)
                     /gene="creC"
                     /locus_tag="ACIUS0_000760"
     CDS             complement(22197..23621)
                     /gene="creC"
                     /locus_tag="ACIUS0_000760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGB0218639.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23621..24310)
                     /gene="creB"
                     /locus_tag="ACIUS0_000761"
     CDS             complement(23621..24310)
                     /gene="creB"
                     /locus_tag="ACIUS0_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGB0218640.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24323..24796)
                     /gene="creA"
                     /locus_tag="ACIUS0_000762"
     CDS             complement(24323..24796)
                     /gene="creA"
                     /locus_tag="ACIUS0_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGB0218641.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25007..25876
                     /gene="robA"
                     /locus_tag="ACIUS0_000763"
     CDS             25007..25876
                     /gene="robA"
                     /locus_tag="ACIUS0_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGB0218642.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25873..26520)
                     /gene="gpmB"
                     /locus_tag="ACIUS0_000764"
     CDS             complement(25873..26520)
                     /gene="gpmB"
                     /locus_tag="ACIUS0_000764"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGB0218643.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26572..27092
                     /gene="yjjX"
                     /locus_tag="ACIUS0_000765"
                     /pseudo
     CDS             26572..27092
                     /gene="yjjX"
                     /locus_tag="ACIUS0_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27177..27503)
                     /gene="trpR"
                     /locus_tag="ACIUS0_000766"
     CDS             complement(27177..27503)
                     /gene="trpR"
                     /locus_tag="ACIUS0_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGB0218644.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27593..29530)
                     /gene="sltY"
                     /locus_tag="ACIUS0_000767"
     CDS             complement(27593..29530)
                     /gene="sltY"
                     /locus_tag="ACIUS0_000767"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGB0218645.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29741..31408
                     /gene="ettA"
                     /locus_tag="ACIUS0_000768"
     CDS             29741..31408
                     /gene="ettA"
                     /locus_tag="ACIUS0_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGB0218646.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31515..31682)
                     /gene="nadS"
                     /locus_tag="ACIUS0_000769"
     CDS             complement(31515..31682)
                     /gene="nadS"
                     /locus_tag="ACIUS0_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGB0218647.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31716..32948)
                     /gene="nadR"
                     /locus_tag="ACIUS0_000770"
     CDS             complement(31716..32948)
                     /gene="nadR"
                     /locus_tag="ACIUS0_000770"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGB0218648.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32969..34351)
                     /gene="radA"
                     /locus_tag="ACIUS0_000771"
     CDS             complement(32969..34351)
                     /gene="radA"
                     /locus_tag="ACIUS0_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGB0218649.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34400..35368)
                     /gene="serB"
                     /locus_tag="ACIUS0_000772"
     CDS             complement(34400..35368)
                     /gene="serB"
                     /locus_tag="ACIUS0_000772"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGB0218650.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35474..36118
                     /locus_tag="ACIUS0_000773"
     CDS             35474..36118
                     /locus_tag="ACIUS0_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGB0218651.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36146..37161
                     /gene="lplA"
                     /locus_tag="ACIUS0_000774"
                     /pseudo
     CDS             36146..37161
                     /gene="lplA"
                     /locus_tag="ACIUS0_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37193..37456
                     /locus_tag="ACIUS0_000775"
     CDS             37193..37456
                     /locus_tag="ACIUS0_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0218652.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37617..38336)
                     /gene="deoD"
                     /locus_tag="ACIUS0_000776"
     CDS             complement(37617..38336)
                     /gene="deoD"
                     /locus_tag="ACIUS0_000776"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGB0218653.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38393..39616)
                     /gene="deoB"
                     /locus_tag="ACIUS0_000777"
     CDS             complement(38393..39616)
                     /gene="deoB"
                     /locus_tag="ACIUS0_000777"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGB0218654.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39668..40990)
                     /gene="deoA"
                     /locus_tag="ACIUS0_000778"
     CDS             complement(39668..40990)
                     /gene="deoA"
                     /locus_tag="ACIUS0_000778"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGB0218655.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41117..41896)
                     /gene="deoC"
                     /locus_tag="ACIUS0_000779"
     CDS             complement(41117..41896)
                     /gene="deoC"
                     /locus_tag="ACIUS0_000779"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGB0218656.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42154..43695
                     /locus_tag="ACIUS0_000780"
     CDS             42154..43695
                     /locus_tag="ACIUS0_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGB0218657.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43667..44530
                     /locus_tag="ACIUS0_000781"
     CDS             43667..44530
                     /locus_tag="ACIUS0_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGB0218658.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44744..45526)
                     /locus_tag="ACIUS0_000782"
     CDS             complement(44744..45526)
                     /locus_tag="ACIUS0_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGB0218659.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45523..46596)
                     /locus_tag="ACIUS0_000783"
     CDS             complement(45523..46596)
                     /locus_tag="ACIUS0_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGB0218660.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46718..46879)
                     /locus_tag="ACIUS0_000784"
     CDS             complement(46718..46879)
                     /locus_tag="ACIUS0_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGB0218661.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46996..47601)
                     /gene="osmY"
                     /locus_tag="ACIUS0_000785"
     CDS             complement(46996..47601)
                     /gene="osmY"
                     /locus_tag="ACIUS0_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGB0218662.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47994..49583)
                     /gene="prfC"
                     /locus_tag="ACIUS0_000786"
     CDS             complement(47994..49583)
                     /gene="prfC"
                     /locus_tag="ACIUS0_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGB0218663.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49674..50351)
                     /gene="yjjG"
                     /locus_tag="ACIUS0_000787"
     CDS             complement(49674..50351)
                     /gene="yjjG"
                     /locus_tag="ACIUS0_000787"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGB0218664.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50366..50812)
                     /gene="rimI"
                     /locus_tag="ACIUS0_000788"
     CDS             complement(50366..50812)
                     /gene="rimI"
                     /locus_tag="ACIUS0_000788"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGB0218665.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50781..51194)
                     /gene="holD"
                     /locus_tag="ACIUS0_000789"
     CDS             complement(50781..51194)
                     /gene="holD"
                     /locus_tag="ACIUS0_000789"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGB0218666.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51297..52328
                     /gene="rsmC"
                     /locus_tag="ACIUS0_000790"
     CDS             51297..52328
                     /gene="rsmC"
                     /locus_tag="ACIUS0_000790"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGB0218667.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52605..52691
                     /locus_tag="ACIUS0_000791"
     tRNA            52605..52691
                     /locus_tag="ACIUS0_000791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52639..52641,aa:Leu,seq:cag)
     gene            52720..52806
                     /locus_tag="ACIUS0_000792"
     tRNA            52720..52806
                     /locus_tag="ACIUS0_000792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52754..52756,aa:Leu,seq:cag)
BASE COUNT        12570 a        14288 c        13245 g        12706 t
ORIGIN      
        1 aaattatcag tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt
       61 ttttgaaccc agtgacgttg aatattactt tgaaccagtg acgatacagg agtcaggaac
      121 cagcatatta aaaaatgagt tagaaaacag tgaggacggt gcgggtcaaa taggctttca
      181 actctccaat gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca
      241 gcatccccat gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa
      301 caatgtctca agcgggcaaa taatgagccg agtcaaaatc gtggtaatgt acaactaatc
      361 tatctccccc accttaactt tgggggagct gccgattaac gcaccgctgg actaaaaagc
      421 gcagaataat ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca
      481 aaaatagcat ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta
      541 tcggcataaa agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcatcgca
      601 ccaaaagctt tcatcaaagc ggcatcatca aactcgccga ggatttctac gtttaatccc
      661 tgggagttaa accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa
      721 agtcgccgtt cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag
      781 aaactcacgc cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct
      841 atcggacagt cagaaatgat catatccagt ttatgctgac ttaattgctc cagcagcatt
      901 tcgtgggtgg attcgaagca gcgaagatga atgggctcgc cttctaccac tgcggcgtta
      961 agtacgctac tgaccaggcg tttggaaagt gcatcagcca cgccaacgtc aaacaataaa
     1021 ttggattctt tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta
     1081 tcggcatagc gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt
     1141 ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt
     1201 ggagttaaat aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag
     1261 aagtaataca agtggttgta attgatatga gacatgctca tttctctccc tgataacaat
     1321 gaaaagggag ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac
     1381 gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga
     1441 gtttcgccca gttaatcagt tctggatcta cgctaccaaa ggccaggctg gcaataaaga
     1501 tagacatagt aaaaccgata ccgcacagga tccccaccac cataatttgc tgataagtcg
     1561 ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc
     1621 ccagcggttt gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc
     1681 catccagcgt gacgccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa
     1741 tcagatacgc cacccacggg tgcaacacat gctccagtcg cttcgctgga gaacgcccat
     1801 gcttctcttt caaaggaata aagaagccga caattacccc cgccagagtt gcgtgaaccc
     1861 ccgatttcaa caccgcagtc cacaacacca cgccaacaag aatatagacg cccgtgcggc
     1921 gtacaccaca cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaaggg
     1981 aggccatcga taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt
     2041 cgataatagc cagagccatc aaaaagatct tcagcactaa cggaacacga cttcccaaca
     2101 gcgccagtac accaagtgca aaagcaatgt cagtagccgc cgggatcgcc cacccttcgc
     2161 gggtaatcgg atcggcatag ttaaaagcca gatagagtaa tgccggcaca atcatcccac
     2221 caatagcggc gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca
     2281 gttcacgttt aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcatcattta
     2341 tccataacag catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct
     2401 ccagaaagtc gtgataccat ccactggttg cgccgctgtt ggccataatc atcgccagga
     2461 tagcggcaat gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat
     2521 gtttcacttt ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcacg
     2581 aatcatcgtt aaaatcagat tatagtgtag gaataattcg ttttttacga gccattgaag
     2641 aatttgcgcc atcagcggtg agatgcgttt ttgacggctg taagcgctca cgagtgaccc
     2701 taagcccaga taatcatagc ttaaggtgag gattcctgag ggattaatta ggaaaagtaa
     2761 taattaattt gttgtcagtc ggcgatgctt gttaggccaa tatttcaaaa ataatccagc
     2821 ttgtcgtcaa taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa
     2881 attacgccac aaaaatgcga ggcgtcttca ggattagaat cgataaaaca aaatgggtta
     2941 aagagagcga tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac
     3001 ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggcct cctatatgct
     3061 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
     3121 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
     3181 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
     3241 tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca
     3301 ggtgctcgca tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt
     3361 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
     3421 gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc
     3481 agctgtttct gcttttcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
     3541 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaatagctta
     3601 ccggtctggg tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg
     3661 atttcgccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg
     3721 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
     3781 ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
     3841 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     3901 catttgttgc acggatcttt gatcagcgta ccgcggccct gacagtgtgg acaggtctgc
     3961 tgtacagcga agaatccctg gcgcatctgc acctgaccag aaccatgaca ggtcggacag
     4021 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     4081 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     4141 ttatagcgta aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata
     4201 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accaccgccg
     4261 cccatgcctc cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc
     4321 gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg
     4381 tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttctgatt
     4441 tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc
     4501 ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt
     4561 cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc
     4621 gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg
     4681 ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg
     4741 cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag
     4801 cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg
     4861 cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa
     4921 cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc
     4981 gttcagacca gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt
     5041 cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat
     5101 gccgcgcggt gccgggttga taccatctag gttgaactga cccagagatt tgttatcagc
     5161 cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc
     5221 ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt
     5281 catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac
     5341 gtcttttacg tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc
     5401 gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac
     5461 cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag
     5521 gccagcatcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac
     5581 caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc
     5641 tgcagtgatg tatggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc
     5701 tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc
     5761 ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga agtcttcacc
     5821 acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc
     5881 gtcgatttcg ataatagaaa tatcgaaagt accacccccc aggtcataaa ccgcgatagt
     5941 acggttgcca gtgcctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat
     6001 gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc
     6061 atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc
     6121 ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt
     6181 ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat
     6241 gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat
     6301 cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag
     6361 agtttcacca tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt
     6421 ctccagcacg cgaggaatgg tgccatccat aatcgctaca caagagttgg tagtacccag
     6481 gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg
     6541 ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc
     6601 agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag
     6661 atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga caagacgtaa
     6721 aattgacagc aattttattc atataaagaa tgaatcgcag ccaaggcgtt catcaaaaaa
     6781 ttgtaataaa aagaaaagat tacgtgcctg aatcttctct ttatcagcag taaactagtg
     6841 ggtattcatc cccctacctc ttcccactaa gagaatcctt atgaaatccg tttttacgat
     6901 ttccgccagc ctggcgatta gcctgatgct gtgctgcacg gcgcaggcaa acgaccataa
     6961 actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac gatctggcgc tgaaacttcc
     7021 tgtttgtcgc attgtgaaac gcatacaact ctctgccgac catggcgatt tacagttaag
     7081 tggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc
     7141 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa
     7201 acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac
     7261 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     7321 attacctgaa tgattcagat ctggattttc ttcagcattg tagtgaggaa cagttggcaa
     7381 atttcgcccg attgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcatactga
     7441 tgcgcaacga actgtttaaa tcgatggaag ggcatcccga gcaacatcgc cgcaactggc
     7501 agctgattgc cggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg
     7561 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaagcgattg aagctgaaag
     7621 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     7681 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     7741 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     7801 gtgtgtcgca tttgctttcc agccaactga cccgcatttt acgcacccac gcagcaatga
     7861 gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg aggccctgtg ggcgcagcat
     7921 taaatggggt taaagcggtc agcggcagca cctatcgcgt gacgattcca gccgtactgc
     7981 aaatcgcctg cctgcgccgg atggttagcg ccactcaggt ctgataccgg aaaccgggga
     8041 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     8101 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     8161 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     8221 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt
     8281 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     8341 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     8401 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     8461 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     8521 actctgaaag gcgcgcgcgt cctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     8581 ctgctggcga tcggtaacat tgccggtaat gccgcgatca tccactttgc cggctggatt
     8641 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtgct gaacgagcag
     8701 tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgtcttgaga tccccccgct
     8761 cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgcagcaa
     8821 ccacttctgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     8881 tggcaaaaat accatgcacc acaacgttac cctcagcgtc cttcacacct tccagcgtct
     8941 ctttaataga cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacgc
     9001 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     9061 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     9121 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     9181 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     9241 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgctggaatg cctttatcct
     9301 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     9361 tcaagatttt tccgtttaaa caatctcgtt aaacagaatg atacactgcg aagggagtga
     9421 cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     9481 gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt
     9541 ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag
     9601 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     9661 cttcgctctc cgccagctct ttcagcagtg ccggtgcgat ggtcagacgg tcacagcctg
     9721 ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac
     9781 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     9841 gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaacg
     9901 gcgagatcag gaacacgccc gcctccgcac aagcacgagc ctgagcgaag gagaacagca
     9961 gggtcaggtt acagttgata ccttcttttt ccagctgttc tgcagcacgg ataccctgcc
    10021 aggtagaagc cagtttgatc agaatacgat cgttgctaat acctgcatcg ttgtagagtt
    10081 tgatcaggcg ttttgctttc gcaattgacg cttcagtgtc ataggaaaga cgcgcatcaa
    10141 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
    10201 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
    10261 cgacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag
    10321 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
    10381 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
    10441 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
    10501 gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca
    10561 ctgcgttgat atcgcccacc atttttatac aaaacctcat gtatgctacg cagaagttat
    10621 caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca
    10681 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
    10741 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
    10801 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
    10861 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
    10921 gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg accaggtgcc
    10981 gtcttttgga tgtgggttgc tgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
    11041 cttgcacaac tttataaaga acgtgacgtc aatgggcagt ttcgtggcgg accggcatgg
    11101 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc
    11161 atcgcctacg gcataatttt cagcggagtt caggcgaacg ccgttgcccg agccctgagt
    11221 ttttcttttg attttcctcc gctggtgacg ggcattattc tcgctgtctt tgctctgctg
    11281 gcaatcactc gtggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
    11341 gcgataatct gggtactgac cagcctggtg atttgcgtaa taaatatcgg gcaacttccc
    11401 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
    11461 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
    11521 gaggtgggaa tgggatcgac gccaaacgcg gcagcggcag ctgcatcctg gcctccgcat
    11581 ccggcagcac aagggattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
    11641 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
    11701 ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctaggg tgctgaattt
    11761 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
    11821 gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc
    11881 tgcaccttcg caacggtcat cggcggcacc ttgctaagcc ttccgctgat gtggcaactg
    11941 gcagatatca taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
    12001 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
    12061 gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctccgga cgcgtgggat
    12121 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
    12181 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
    12241 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
    12301 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
    12361 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
    12421 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
    12481 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
    12541 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
    12601 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
    12661 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
    12721 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
    12781 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
    12841 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
    12901 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
    12961 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
    13021 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
    13081 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
    13141 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
    13201 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
    13261 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
    13321 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
    13381 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    13441 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
    13501 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
    13561 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
    13621 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
    13681 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
    13741 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
    13801 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
    13861 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
    13921 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
    13981 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
    14041 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
    14101 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
    14161 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
    14221 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
    14281 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
    14341 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
    14401 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
    14461 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
    14521 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
    14581 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
    14641 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
    14701 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
    14761 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
    14821 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
    14881 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
    14941 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
    15001 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
    15061 agagcgaaca aggtcgggcc ggagccggag ataccgctcg ctaccgcgcc gatttccgcg
    15121 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
    15181 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
    15241 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
    15301 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
    15361 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
    15421 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
    15481 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
    15541 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
    15601 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
    15661 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
    15721 agtttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
    15781 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
    15841 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
    15901 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
    15961 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
    16021 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
    16081 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
    16141 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
    16201 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
    16261 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
    16321 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
    16381 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
    16441 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
    16501 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
    16561 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc
    16621 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
    16681 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
    16741 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
    16801 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
    16861 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
    16921 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
    16981 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
    17041 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
    17101 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
    17161 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
    17221 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
    17281 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
    17341 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
    17401 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
    17461 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
    17521 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
    17581 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
    17641 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
    17701 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
    17761 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
    17821 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
    17881 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
    17941 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
    18001 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
    18061 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
    18121 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
    18181 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
    18241 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
    18301 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
    18361 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
    18421 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
    18481 gttgtgtact ctgtaatttt tatctgtttg tgcgctatgc ctatattggt taaagtattt
    18541 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
    18601 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
    18661 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
    18721 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    18781 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
    18841 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    18901 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    18961 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    19021 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    19081 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    19141 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    19201 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    19261 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    19321 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    19381 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    19441 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    19501 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    19561 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    19621 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    19681 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    19741 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    19801 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    19861 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    19921 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    19981 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    20041 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    20101 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    20161 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    20221 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    20281 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    20341 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    20401 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    20461 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    20521 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    20581 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    20641 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    20701 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    20761 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    20821 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    20881 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    20941 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gagggtaaac
    21001 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    21061 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    21121 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    21181 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    21241 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    21301 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    21361 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    21421 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    21481 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    21541 gccatattca gctgcaggtt ctgcttccgc cagtcccctt cgggtaaagg gatatgcacg
    21601 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    21661 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    21721 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    21781 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    21841 acgttctgat tgccatcaac catcaatgac tcaggtaacc aaaaatggat aaaacttcgt
    21901 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    21961 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    22021 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    22081 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    22141 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    22201 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    22261 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    22321 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    22381 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    22441 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    22501 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    22561 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    22621 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    22681 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    22741 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    22801 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    22861 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    22921 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    22981 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    23041 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    23101 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    23161 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    23221 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    23281 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    23341 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    23401 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    23461 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    23521 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    23581 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    23641 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    23701 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    23761 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    23821 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    23881 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    23941 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    24001 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    24061 gggccgtcag gaacagtaca ggtaacgccg gatagagcgc cagtaactgg cggcacaatt
    24121 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    24181 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    24241 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    24301 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    24361 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    24421 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    24481 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    24541 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    24601 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    24661 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    24721 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    24781 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    24841 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    24901 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    24961 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    25021 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    25081 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    25141 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    25201 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    25261 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    25321 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    25381 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    25441 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    25501 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    25561 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    25621 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    25681 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    25741 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    25801 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    25861 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    25921 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    25981 cgaaatagaa cagttacgca gacgtaagcg gcgctctgcc catgctggta atccgagaat
    26041 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    26101 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    26161 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    26221 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    26281 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    26341 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    26401 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    26461 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    26521 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    26581 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    26641 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    26701 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    26761 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    26821 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    26881 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    26941 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    27001 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    27061 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    27121 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    27181 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    27241 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    27301 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    27361 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    27421 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    27481 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    27541 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    27601 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    27661 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    27721 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    27781 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    27841 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    27901 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    27961 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    28021 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    28081 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    28141 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    28201 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    28261 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    28321 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    28381 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    28441 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    28501 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    28561 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    28621 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    28681 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    28741 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    28801 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    28861 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    28921 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    28981 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    29041 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    29101 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    29161 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    29221 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    29281 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    29341 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    29401 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    29461 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    29521 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    29581 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    29641 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    29701 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    29761 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    29821 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    29881 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    29941 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    30001 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    30061 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    30121 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    30181 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    30241 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    30301 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    30361 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    30421 ctggatcctc gaactggacc gcggtgaagg aattccgtgg gaaggtaact actcctcctg
    30481 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    30541 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    30601 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    30661 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    30721 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    30781 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    30841 tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa
    30901 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    30961 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    31021 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    31081 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    31141 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    31201 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    31261 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    31321 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    31381 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    31441 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    31501 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    31561 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    31621 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    31681 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    31741 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    31801 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    31861 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    31921 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    31981 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    32041 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtgac ccaacgcgat tttatcgtag
    32101 tcggaatact gcaatgcgat ttcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    32161 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    32221 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    32281 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    32341 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    32401 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    32461 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    32521 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    32581 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    32641 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    32701 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    32761 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    32821 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    32881 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    32941 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    33001 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    33061 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    33121 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    33181 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcggcgctgg
    33241 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    33301 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    33361 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    33421 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    33481 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    33541 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    33601 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    33661 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    33721 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    33781 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    33841 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    33901 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    33961 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    34021 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    34081 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    34141 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    34201 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    34261 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    34321 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    34381 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    34441 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    34501 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    34561 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    34621 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    34681 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    34741 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    34801 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    34861 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    34921 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    34981 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    35041 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    35101 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    35161 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    35221 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    35281 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    35341 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    35401 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    35461 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    35521 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    35581 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    35641 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    35701 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    35761 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    35821 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    35881 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    35941 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    36001 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    36061 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    36121 cattactaca agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta
    36181 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    36241 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    36301 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    36361 tggcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
    36421 caagccggag tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc tgaacgcgct
    36481 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
    36541 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
    36601 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
    36661 actggcggcg aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct
    36721 gccggggctc accatgagca ggtttgcgag gccataaccg aggccttttt cgcccattat
    36781 ggcgagcacg tggaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    36841 gccgaaacct ttgcccgtca gagtagctga gagtggaact tcggtcaggc tccggcattc
    36901 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    36961 aaaggccata tcacccgcgc ccaggtattt accgacagcc tcaaccccgc gccgctggaa
    37021 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    37081 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    37141 atagcggggg cggtaaggta actcgctgat taaaatatcc aaggaaggaa atatgatcga
    37201 tctggaaaac caggaacggg agattatcaa tttaatgttc agccagcgta tttcctggct
    37261 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    37321 ctcgattaat tctctaaaac agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    37381 caaaatggca gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    37441 ggctgaacat aaatgaatag attcgtgaat agatacttga atgtttttta ttttctatcc
    37501 atatgaaaaa taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag
    37561 ccggagcagt cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac
    37621 tctttatcgc ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc
    37681 tgacgctcag cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac ggtgcagatg
    37741 gtcagggctt tcgcgccaaa ctctgcagcc acgccgtaga taccagccgc ttccatttcc
    37801 acgccgagga tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac
    37861 aggtcagcgg agaacaggtt acccacgcga gcatcaacgc ccagtgcttt agctgcatct
    37921 actgcgttac gcaccatgtc aaagtcagcg atagcggcaa agtcatggtc tttaaaacgg
    37981 atgcggttaa ctttggaatc ggtgcaggcc cccataccga taacgacgtc gcgcagtttt
    38041 acgtgcggca gaactgcgcc acaggaacca acgcggataa ttttcttcac gccgaaatcg
    38101 gtgatcagtt ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg
    38161 gaaattttgc ggcctttgta agtaccagtg aagcccagca taccgcgaac gttgttcact
    38221 tcacgggcat cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc
    38281 atcaaaacta cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt
    38341 ttatcctttt tgtgacataa caaaggcgga gtcaacgctc cgcccaaatc catcagaaca
    38401 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    38461 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggcca tataccagta
    38521 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcgcaa ccgtggtcag
    38581 cggtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    38641 cgaacagttc cagacccgca gcataaccgg cgacgtcgcg gcggtggccc caggaagagt
    38701 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    38761 tggtggcgtc aaacagcgcg tccaagccag tcgctttcac ttttttggtg ataccgcagt
    38821 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg gccgtgtttt tcatcaacca
    38881 gtttctgcag cacggtcggt gctggcggct caacagccaa gtcgtgacgg ttaccggtac
    38941 gctggaagtt accagctttg tcaccgataa acggacgagc gataacacga ccgatattgt
    39001 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccagac
    39061 cgaaagtttc ttcatggcag gcaatctgga acacggagtc agcggaggta tagaaaatcg
    39121 gcttgccggt tttcatgtgc tcttcgccca gttgatccag aatgaccgta ccggaagagt
    39181 ggcagttacc gaggtaaccc ggcagattag cgcgttcgac cagtttatcc agcagctctt
    39241 gcgggaagct gttttcgtga tcggagaaat atccccactc aaacagaacc gggacaccgg
    39301 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    39361 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    39421 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcggc cctttacgac
    39481 cattatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    39541 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    39601 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    39661 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gcgttatcgg
    39721 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    39781 cgaggataac cgccagcgga cgctgaccgt ctacctggtc gcccagacgc gccatatcag
    39841 taaagccgac gctgtaatcg atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    39901 ctactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    39961 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    40021 tcgggccttt ttgtgccgct accatacgac caaagacttc tgccgcttta ccgttgtcca
    40081 gcaccgcctg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagatca
    40141 acatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    40201 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    40261 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    40321 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    40381 tcacgtccat caccagcgcg tccagacctt ccgccagttt cttcgccagg atcgaggcag
    40441 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    40501 cagccggagc cagtgaactg gtctgaccga taatcgccac gccgacgtct ttaataattt
    40561 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    40621 gcgtaccgcc agtatgaccg aggccgcgac cggagatcat cggaatatag ccgccgcagg
    40681 ctgcgaccat cggccccaac atcagcgaag tgacatcgcc gacgccgccg gtcgagtgtt
    40741 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    40801 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    40861 tcgcgagggc ggcaatctgc ccttcggaaa tagtgttgtc gcgaataccg ttgataaaga
    40921 aacgaatttc ttcatcgctc agcgcatgac catcacgttt tttacgaata atttcttgtg
    40981 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    41041 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    41101 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcgccgtgg cccagcgctt
    41161 tcagcaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tcagcccagt
    41221 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    41281 cgccgcccgc cggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    41341 tcatgatgcg agcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    41401 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    41461 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    41521 tacaggcttt caccaggtca aaaccaacct gctcgtcacc cgccatcagc gcgcggtacg
    41581 ggaacaccac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    41641 cgatttcgat gtcgtcgtta ccgtgtggga agttggttac cgtagcgata cggatttccg
    41701 gggtgccctg ctctttcaga gttttgcgag caatcgggat aaaacgagga tagatacaga
    41761 tagcggcggt attgccgacc ggagttttgg cctgatgaca cagagcaatt actttctcgt
    41821 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    41881 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    41941 tgttagtatt ctaacatcta ctccgcaaca cacttcgata cacatcacaa ttaaggaaat
    42001 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    42061 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    42121 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    42181 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    42241 tttctggcac tggaagcaga aaacaatctg ccttacccac agcttcctgc cgaagcccgc
    42301 cgcgcgctgg atgaaggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    42361 cgctatgtct tacccgatta cgctcgtttt ctggcgaacg gttccgaatg gctggagctg
    42421 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    42481 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccgtat
    42541 gttagaattc taacacaaaa cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    42601 gacagaaccc tgccagatgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    42661 acccgcgcga tcttacgtgc agacgcgcag gtttcaccga acctgacctt tatctacgat
    42721 cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc
    42781 aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg
    42841 attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt
    42901 aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta
    42961 ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa
    43021 caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt
    43081 tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgagaaa
    43141 gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc
    43201 agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc
    43261 gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt
    43321 ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat
    43381 gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac
    43441 ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc
    43501 gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat
    43561 ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc
    43621 gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg
    43681 cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc
    43741 tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg
    43801 acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt
    43861 tgacggcaaa gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagat
    43921 gtgtccgcaa catgccacac caatggcgca atccatgagc gtggacgaag tgcttagcca
    43981 tgtgcgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac
    44041 ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca
    44101 tctcacctgt ctggtggaca gtaacggtat gttgagcgaa accggctggg aaaaattact
    44161 cccggtgtgt gacggcgcaa tgctcgatct caaagcatgg ggtagcgaat gtcatcaaca
    44221 attaaccgga cgcgataatc aacagattaa gcgcagcatc tatttgctgg cagagcgcgg
    44281 caagctggcg gaactgcgtt tgctgatgat cccaggccag gtggattatt tgcaacacat
    44341 cgaagaactg gcggcgttta tcaagggact tggtgatgtt ccggttcgcc tgaacgcgtt
    44401 tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt
    44461 tgagccgttg gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct
    44521 ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg cctgataaga
    44581 cgcgtcaagc gtcgcatcag gcattgtgcg ccaactgccg gatgcggcgt gaacgcctta
    44641 tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgttaag cgtcgcatca
    44701 ggcattatgc gccgactgcc ggatgctgcc tacgaagggc aaactaccca cgaacgttaa
    44761 acaccgcata cgtgttatta agcaacactt cggcaatctc atccgcgggt tccgggcgta
    44821 actcgcaaag cacggcaaac acacgggcag cctgttccgg gcggttaggc tgcccctgaa
    44881 aaccgttgag cggcatatcc ggcgcatcgg tttccagcaa caacgatgcc agcggtaatt
    44941 ttgcgatgac atcgcgggtt ttactggcgc gtggataggt gatagtaccg cctacgccga
    45001 ttttgtagcc cagctgtaca aaccgttcag cctgttgctg gctgccagaa aatccgtgga
    45061 caacaccagc acgcggcaaa tcgtggcgtt taagatgcat cgccagtttg tcgtgcgtgc
    45121 gccgtgaatg caggatcacc ggcagatcgt agcgtttcgc cagtttcagt tgttcgtcga
    45181 gtaaccactg ctgcctctca aattgcggat cgtcgccgaa gagatccaga ccgatctccc
    45241 ccaccgccac caccttcgcc ggacgccttt ccagcgcctg ctgtagctgc tcaagagaca
    45301 catcgctatg tttttccaac ataccaggat gcaagcccaa tacggcatac agcggttgat
    45361 aattttccgc taacgcctgc acacgggcaa aattttccgc ctctgtcgcc ggaacaatga
    45421 tcttgcctac gcccgcttgt gccgcgcgtt gcaggctggc ctcttcatcg ccaataaaag
    45481 gcgggaaatc aaaatggcag tgagtgtcga taaaacggca aatcaagcca gatcctcatt
    45541 gttaaatgtc gggtcgttcg cctgcggagc gtcgctcact tcggcaacca gcgtatcgtt
    45601 ggcgactggc gcaggcggaa tgacaatcga ttccggcgtc accactggca ccagatggcg
    45661 ggtaagcggc gctttttcag ttaatagctt gccaaccgtg gcgaggaaat aacgaccgca
    45721 taaacgccca agtttatagt cttcacgcag cgccggaatc cgactgccaa gcgcgatact
    45781 atgtaatggc ttcggcggat aaatttcgga tatccgcagc ttgcccggtg gtttctcaat
    45841 aaattgctga atgtcacgat agctggtttc atgatgctgc accagattga ccagcggctg
    45901 caggctactg tcacccagcc agcgttccat gcgtttgaac cactgcggcg tgtagtacat
    45961 ttgtgacggc acagtgcgga tgacgaccaa cgttttagcg ccctgccttg ccgcctcttt
    46021 aaccggaatc gcatcactga tcccgccatc caggtagtta atgccttcca gcgacactcc
    46081 gctacgataa aagccaggta tcgcactgga ggcgcgaatc acatccagcc agttttgttt
    46141 ggttggtaaa aagtaattcg gcgcgtagtc atcctgacga caggcgcaca tataaaacga
    46201 tttgccgctg tcaaacaacc gcgcggcggt atccatttgc aacggcatct ggcttgcggt
    46261 ggcctccacc agccagtcga gatcgataag atttcctcca cggacaaagc gcaatggatc
    46321 gaaaaattcg cgttttgtgg tatagcgcat gatgactttg cgcgcgtaac cgggctgatt
    46381 gcagataaac gccgagaggt tctgcgcccc ggcagatgtg ccgagataaa gatcgaaagg
    46441 attaaactgc gcgcgcataa actcatccag cacgccagcc gtgaaaattc cacgctgtcc
    46501 gccgccttcg cacaccagag ctattcgtcc aggctggaac ggccttagcg acaacggcgc
    46561 aatattacca agcgttacag gtattcgctg ccccacttct gccttcctgt ttttgttgtg
    46621 aaagaaagac aaagtaacgc aatttattat tcgctaaaac cgcaaaagcc agtccgctgg
    46681 actggctttg gataaaattt tcttaatggt ctgaaatcta gggtcgtttt cggcccatga
    46741 acaaactcac taggaacaga ataatcccga cgacaaagac aattttagct gcgcctgcag
    46801 cggtaccggc cagaccacca aacccaagtg cggcggcgat taacgcgata accagaaata
    46861 tgatgcccca acgaaacata agcgtctcct ttaccatagt taatgtcacc gcttgatatg
    46921 agcgtgaaaa tcactcaatg cgatattttt agtgtggtgc acatcgcgcc tcccgacaaa
    46981 gttcgggagg acgaattact tagttttcag atcattttta acgcttttca caccatctac
    47041 cgctttggcg atactttcag cacggtcact ttgtgcctga gaatctacgg taccggagag
    47101 ctgaaccacg ccatcggtgg tttcaacttt cactttacgt gaagggacga tatcgtccgc
    47161 cagcagtttg gctttgattt cactggtggt ggcggtgtca cccgcgtagc ccttcaccga
    47221 gccttcttta gcgtcgcgaa cgtgcagttt gtcgctgaca gaggtcacgc cttcaacgcc
    47281 tttcgccact ttcactgcct cttcggcctg ggcctgactt tcaacgaaac cgctcagggt
    47341 cacgactttt tgatcggttt tgacagagat atcggtgctc ttgatgttgt catgatccac
    47401 cagcgccgcc ttcactttcg cggtgatggc gctgtcatcc atgaaattac cgactttatt
    47461 catagagcta tcgacttttt gccctgcgct ttcattggta gtctgcgcgt tgttttccgc
    47521 gtaggcagag ccggtcgcga cggcagaggt caacattaca gccagcagag ttttcgaaat
    47581 cttcagtctt gtcatagtca tcgatttatt cctgtatgtt tgctcgtaat ttgagcctag
    47641 caacacgagg ttgcattgct gaatagggag agacttcacc ctctacagaa gtcaatggtc
    47701 gccatcacaa aagcgatggg tgatgaataa cgaccattac agcctctgaa tcagttatta
    47761 atatcggtag aatgacaatc gacggcttta gatactaata tctacgcatt gaacggtatt
    47821 taacgccatc agaaatgtca tcactttgtt aaatatagat cacaattttg aaaccgctcg
    47881 ggatatcagc gaaaacataa gcaaaagtga atgttttaag aacattccgt aagcggctaa
    47941 taaggaaggg aaattgacag ggcgcagcgg ctaccgcgcc ctggaggcaa gaattaatgc
    48001 tcgcgggtct ggtggaactg aacgtccgga taacgttcct gtgccaggcg caggttgacc
    48061 atgctggtag cgatgtaagc gaggttatcg ccgccatcaa gcgccagttg gctttcgttc
    48121 ttacgcttga actcttcgaa tttcttcgcg tctgcacatt ctacccagcg ggcagtggca
    48181 acgttgactg actcatacac tgcttcaacg ttgtattcgc tcttcaggcg cgctaccacc
    48241 acatcaaact gcagcacacc aaccgcacca acgatcagat cgttgttgga gattggacgg
    48301 aacacctgca ccgcgccctc ttcggaaagc tgtaccagcc ctttgagcag ctgtttttgc
    48361 ttcagcggat ctttcaggcg gatacgacgg aacagttctg gtgcgaagtt cggaataccg
    48421 gtgaacttca tcatctcacc ctgggtaaag gtgtcgccga tctgaatggt gccgtggttg
    48481 tgcaggccga ggatatcgcc cggatacgct tcttcaacgt gcgaacggtc acccgccata
    48541 aaggtcagcg cgtcggagat caccacatct ttcgcagtgc gcacctggcg cagtttcatg
    48601 cctttttcat atttaccgga caccacacgc ataaacgcca cgcggtcgcg gtgtttcgga
    48661 tccatgttgg cctgaatttt aaatacgaag ccggtaaatt tatcttcgct cgcttctacg
    48721 gtacgggtat cggtctgacg cggcatcggc gcaggcgccc actccaccag gccattcagc
    48781 atatgatcaa cgccgaagtt acccagcgca gtaccgaaga ataccggagt gatttcgccc
    48841 gcaaggaaca gctctttgtc gaactcgtta gacgcgcctt taaccagttc cagttcgtca
    48901 cgcagctgct gtgccagatc ttcaccaacc gcagcatcga gatccgggtt attcagccct
    48961 ttaacaatgc ggacttcctg aatggtgtgc cctttaccgc tctgatagag ataggtttca
    49021 tctttataaa ggtggtaaac gcctttaaac agcttgccgc agccaattgg ccaagtgatc
    49081 ggtgcgcagc caattttcag ctcgttctca acttcatcga gcagttccat cgggtcgcgg
    49141 atatcacggt caagtttgtt cataaaggtg aggatcggcg tgtcgcgcag acgggtaact
    49201 tccatcagct tacgggtacg atcttcaaca ccttttgcgg cgtcgataac catcaggcag
    49261 cagtccaccg ccgtcagggt acgataggta tcttccgaga agtcttcgtg ccccggggtg
    49321 tcgagcaggt taaccaggca atcgtgatac ggaaactgca tcacagacgt agtaatggag
    49381 atcccacgct gcttttccat ctccatccag tccgacttag cgtgctggtt ggaaccacgg
    49441 ccttttactg taccggcggt ctgaatggcc tgtccgaaca gcagcacctt ctcggtgatg
    49501 gtagttttac cggcgtccgg gtgagaaata atggcaaaag tgcggcgctt cgccacctct
    49561 ttcaaataag gagacaacgt cataattctt tcttcttgag taagcggcat cgcgccgcgc
    49621 ttgttgaaaa cgaaaaattg cggctatttt acccatcaac gggggggagg caatcagtgt
    49681 ttacacagga gctgctccag ttcgtgcaac gaagaaacgg tccaggtggg cgcgatgcct
    49741 tctggttgct cgcgattgtg cgcattcagc cagcaggtcg caagcccggc gttgatgcca
    49801 ccgagaatat cggactcggc agtgtcgcca accatcagca cgcgtgaacg gtcaggattg
    49861 cccgcctgtt ccagcgcata atcgaaaatt ttcttattcg gtttggcaac gccaacttct
    49921 tcggaaatca ccagcaaatc gaagtaatca cgcaggcccg tccgttccag acgcacttgc
    49981 tgcaaggcgc taaaaccgtt ggtgatgatg ccgattttag cgttgctacg aatggcgtta
    50041 agcagagaaa ccgcgcccgg caacggcgta cagatttccg ccatcgcatt aataaaggca
    50101 tcgttaagca agcctgcttc aactttcagc cgttcggccc agctctcgaa acgcccgtgc
    50161 tgaagctgta atgaagtgat cgcgccgttt tgataatcca cccacagtgg cttgttaacg
    50221 gcctgatagt cctgaaaatc ttcagcggta aaggtgacgc tgtaatcaag aaacatccgc
    50281 tgcaggccgg tgaatgagtc aaaggtaaac agcgtttcat cggcatcaaa gaaaatccag
    50341 tcccacttca ttattccacc ttgtcttaca tactgattgg caacgccatg atgatggcgt
    50401 cttcgcgacc gtccgtggtg gggtagtaat tgcggcgaat cgtcgcctcg ttaaagccta
    50461 aactttcgta cagggcaatg gcggcagcat ttgaggcacg gacttccagc catagtgtcg
    50521 ccacgccgcg tttttccagt tcgtcgatca gatgttccag cagcgcccta cccagcccct
    50581 gacgctgata gtcgggatcg accgcaatat tgaacaatgt agcttcatcc agcaccactt
    50641 gcgtaatcgc aaacgccgcc attttgccgt tttgcgttaa ctgaaagttg agataacgct
    50701 cgccctggtt gctggcaaag gttttttcac tccacggaaa ggcgtgggcg cgttgttcaa
    50761 tgtggtaagc cgctggtaaa tcagtcgttt cgagggaaga aatcgtgttc atatgtgcaa
    50821 atttgttgcc ataacgcggc gcgtgccgtt gggtttgccc gtaattcggt gagcgccggt
    50881 gatgccacct gagcgccttc caatgatagc ggttcgtcag tacccaaccg ccagctgttg
    50941 cagcgactgc cttgcggcag catcgcgatt ttttctggcg tcagttgcag cacttgatcg
    51001 gggctgacgg ttaatgcgcg cagaacatcg ctcactaaag gatcagtcag ggcgggaaga
    51061 tcgtttgcca ccatcaccag acggacgtgt gccgggctcg caatggcaat ctccccctgc
    51121 aacgcgccag ggcgacgcag cgaccactgg gtaatgccca gttgctgtaa ctgccagtct
    51181 cgtcgggatg tcatagcgaa tcgctcctgt tgtcaggggc gcaaatatag caaattcgtc
    51241 gataccgcgc caacatatgg ctataatcgc cgccagtatc aattgaggag cattccatgt
    51301 ctgcatttac cccggcaagt gaagtcttgc tgcgtcacag tgatgatttc gaacaaagcc
    51361 gtattctgtt tgccggagac ttacaggatg acctgcccgc gcgtttagat accgcggcca
    51421 gccgtgctca tacccagcaa ttccaccatt ggcaggtgtt aagctgccag atgggggata
    51481 acgcccgttt tagtctggtc gccacggcga atgatgtcgc agattgcgat acgctgattt
    51541 actactggcc gaagaacaaa ccggaagccc agttccagtt gatgaattta ctttctctgc
    51601 tgccagtggg gacagatatt tttgtcgttg gcgagaaccg cagcggcgtg cgcagcgctg
    51661 agcagatgct ggcagattat gcgccgttga ataaagtcga cagcgctcgt cgctgtggcc
    51721 tctattttgg tcgtctggaa aaacagccag tatttgatgc ggataaattc tggggcgaat
    51781 acagcgtcga tggcctgacg gtcaaaacgc tgcctggcgt gtttagccgc gacggtctgg
    51841 atgtcggtag ccagttgctg ctctcgacgt taactccgca cacgaaaggt aaagtgctgg
    51901 atgtcggctg tggcgcgggt gtactttcag ttgcctttgc gcgccattcg ccgaaaattc
    51961 gtctcacctt gtgcgatgtc tctgccccgg cggtagaagc cagccgcgca acacttgcag
    52021 ccaacggtgt tgaaggtgaa gtctttgcca gcaacgtctt ttccgaggtg aaaggttgtt
    52081 ttgatatgat catctccaac ccgccgttcc acgatgggat gcaaaccagc ctggatgcgg
    52141 cgcaaacgct gattcgtggc gcggtgcgtc atcttaatag cggcggcgag ctgcgaattg
    52201 tagcgaacgc cttcctgcct tacccggacg tgctggatga gacatttggc ttccatgaag
    52261 ttatcgcgca aaccgggcgc ttcaaggtat atcgcgccat tatgacccgc caggcgaaga
    52321 aaggttgagt atctgatatc tgtcgccgca taccgtgtcg gatgcggcgt gaacgcctta
    52381 tccgacctac aaaacctgca cgttagccct tcgtaggcca gataagacgc gccagcgtcg
    52441 catctggcat ctgcccccgc atgcctacca aacctgcacg ctagtttcct gatggacatt
    52501 tttccagcaa ttacacatca ggcgataatt aactattgac gaaaagctga aaaccactag
    52561 aatgcgcctc cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat
    52621 tggtagacgc gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc
    52681 ccctcgcacc aaaaatcacg ttgatattgc tcgcactggg cgaaggtggc ggaattggta
    52741 gacgcgctag cttcaggtgt tagtgttctt acggacgtgg gggttcaagt cccccccctc
    52801 gcaccaaac
//
LOCUS       DBBBSB010000014        49831 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.014, whole genome shotgun sequence.
ACCESSION   DBBBSB010000014 DBBBSB010000000
VERSION     DBBBSB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49831
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.014"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1225
                     /locus_tag="ACIUS0_000793"
     CDS             <1..1225
                     /locus_tag="ACIUS0_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGB0218668.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1222..2187
                     /gene="glpX"
                     /locus_tag="ACIUS0_000794"
     CDS             1222..2187
                     /gene="glpX"
                     /locus_tag="ACIUS0_000794"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGB0218669.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2209..2718
                     /gene="fumE"
                     /locus_tag="ACIUS0_000795"
     CDS             2209..2718
                     /gene="fumE"
                     /locus_tag="ACIUS0_000795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGB0218670.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2715..3428
                     /locus_tag="ACIUS0_000796"
     CDS             2715..3428
                     /locus_tag="ACIUS0_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGB0218671.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3400..4077)
                     /locus_tag="ACIUS0_000797"
     CDS             complement(3400..4077)
                     /locus_tag="ACIUS0_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0218672.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPVDEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4071..4748)
                     /locus_tag="ACIUS0_000798"
     CDS             complement(4071..4748)
                     /locus_tag="ACIUS0_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0218673.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4736..5443)
                     /locus_tag="ACIUS0_000799"
                     /pseudo
     CDS             complement(4736..5443)
                     /locus_tag="ACIUS0_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5444..6022)
                     /locus_tag="ACIUS0_000800"
     CDS             complement(5444..6022)
                     /locus_tag="ACIUS0_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGB0218674.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6045..6476)
                     /locus_tag="ACIUS0_000801"
                     /pseudo
     CDS             complement(6045..6476)
                     /locus_tag="ACIUS0_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6848..7867
                     /gene="epd"
                     /locus_tag="ACIUS0_000802"
     CDS             6848..7867
                     /gene="epd"
                     /locus_tag="ACIUS0_000802"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGB0218675.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7917..9080
                     /gene="pgk"
                     /locus_tag="ACIUS0_000803"
     CDS             7917..9080
                     /gene="pgk"
                     /locus_tag="ACIUS0_000803"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGB0218676.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9295..10374
                     /gene="fbaA"
                     /locus_tag="ACIUS0_000804"
     CDS             9295..10374
                     /gene="fbaA"
                     /locus_tag="ACIUS0_000804"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGB0218677.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10565..11425
                     /gene="mscS"
                     /locus_tag="ACIUS0_000805"
     CDS             10565..11425
                     /gene="mscS"
                     /locus_tag="ACIUS0_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGB0218678.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11564..12199
                     /gene="argO"
                     /locus_tag="ACIUS0_000806"
     CDS             11564..12199
                     /gene="argO"
                     /locus_tag="ACIUS0_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGB0218679.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12292..13032
                     /locus_tag="ACIUS0_000807"
     CDS             12292..13032
                     /locus_tag="ACIUS0_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGB0218680.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13199..14149
                     /locus_tag="ACIUS0_000808"
     CDS             13199..14149
                     /locus_tag="ACIUS0_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0218681.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14091..15569)
                     /gene="scpC"
                     /locus_tag="ACIUS0_000809"
                     /pseudo
     CDS             complement(14091..15569)
                     /gene="scpC"
                     /locus_tag="ACIUS0_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15593..16378)
                     /gene="scpB"
                     /locus_tag="ACIUS0_000810"
     CDS             complement(15593..16378)
                     /gene="scpB"
                     /locus_tag="ACIUS0_000810"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGB0218682.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16389..17384)
                     /gene="meaB"
                     /locus_tag="ACIUS0_000811"
     CDS             complement(16389..17384)
                     /gene="meaB"
                     /locus_tag="ACIUS0_000811"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGB0218683.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17377..19521)
                     /gene="scpA"
                     /locus_tag="ACIUS0_000812"
     CDS             complement(17377..19521)
                     /gene="scpA"
                     /locus_tag="ACIUS0_000812"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGB0218684.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19725..20618)
                     /gene="argP"
                     /locus_tag="ACIUS0_000813"
     CDS             complement(19725..20618)
                     /gene="argP"
                     /locus_tag="ACIUS0_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGB0218685.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21001..21660
                     /gene="rpiA"
                     /locus_tag="ACIUS0_000814"
     CDS             21001..21660
                     /gene="rpiA"
                     /locus_tag="ACIUS0_000814"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGB0218686.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21916..23148
                     /gene="serA"
                     /locus_tag="ACIUS0_000815"
     CDS             21916..23148
                     /gene="serA"
                     /locus_tag="ACIUS0_000815"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGB0218687.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23377..23436
                     /gene="ibsC"
                     /locus_tag="ACIUS0_000816"
     CDS             23377..23436
                     /gene="ibsC"
                     /locus_tag="ACIUS0_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGB0218688.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23769..23828
                     /locus_tag="ACIUS0_000817"
     CDS             23769..23828
                     /locus_tag="ACIUS0_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGB0218689.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23929..24531)
                     /locus_tag="ACIUS0_000818"
     CDS             complement(23929..24531)
                     /locus_tag="ACIUS0_000818"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGB0218690.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24552..24735)
                     /gene="ssrS"
                     /locus_tag="ACIUS0_000819"
     ncRNA           complement(24552..24735)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACIUS0_000819"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24777..25106)
                     /gene="zapA"
                     /locus_tag="ACIUS0_000820"
     CDS             complement(24777..25106)
                     /gene="zapA"
                     /locus_tag="ACIUS0_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGB0218691.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25274..25852
                     /gene="ygfB"
                     /locus_tag="ACIUS0_000821"
     CDS             25274..25852
                     /gene="ygfB"
                     /locus_tag="ACIUS0_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGB0218692.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25878..27203
                     /gene="pepP"
                     /locus_tag="ACIUS0_000822"
     CDS             25878..27203
                     /gene="pepP"
                     /locus_tag="ACIUS0_000822"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGB0218693.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27200..28378
                     /gene="ubiH"
                     /locus_tag="ACIUS0_000823"
                     /gene_synonym="visB"
     CDS             27200..28378
                     /gene="ubiH"
                     /locus_tag="ACIUS0_000823"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGB0218694.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28401..29603
                     /gene="ubiI"
                     /locus_tag="ACIUS0_000824"
                     /gene_synonym="visC"
     CDS             28401..29603
                     /gene="ubiI"
                     /locus_tag="ACIUS0_000824"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGB0218695.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29677..29760)
                     /locus_tag="ACIUS0_000825"
     CDS             complement(29677..29760)
                     /locus_tag="ACIUS0_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218696.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30051..31145
                     /gene="gcvT"
                     /locus_tag="ACIUS0_000826"
     CDS             30051..31145
                     /gene="gcvT"
                     /locus_tag="ACIUS0_000826"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGB0218697.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31169..31558
                     /gene="gcvH"
                     /locus_tag="ACIUS0_000827"
     CDS             31169..31558
                     /gene="gcvH"
                     /locus_tag="ACIUS0_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGB0218698.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31677..34550
                     /gene="gcvP"
                     /locus_tag="ACIUS0_000828"
     CDS             31677..34550
                     /gene="gcvP"
                     /locus_tag="ACIUS0_000828"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGB0218699.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGQDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34817..35560
                     /locus_tag="ACIUS0_000829"
     CDS             34817..35560
                     /locus_tag="ACIUS0_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0218700.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35617..37050)
                     /gene="bglA"
                     /locus_tag="ACIUS0_000830"
     CDS             complement(35617..37050)
                     /gene="bglA"
                     /locus_tag="ACIUS0_000830"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGB0218701.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37095..37405
                     /gene="yqfB"
                     /locus_tag="ACIUS0_000831"
                     /pseudo
     CDS             37095..37405
                     /gene="yqfB"
                     /locus_tag="ACIUS0_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37569..38228
                     /locus_tag="ACIUS0_000832"
     CDS             37569..38228
                     /locus_tag="ACIUS0_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGB0218702.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38424..39404)
                     /gene="ygfZ"
                     /locus_tag="ACIUS0_000833"
     CDS             complement(38424..39404)
                     /gene="ygfZ"
                     /locus_tag="ACIUS0_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGB0218703.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39647..39913
                     /gene="sdhE"
                     /locus_tag="ACIUS0_000834"
     CDS             39647..39913
                     /gene="sdhE"
                     /locus_tag="ACIUS0_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGB0218704.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39894..40301
                     /locus_tag="ACIUS0_000835"
     CDS             39894..40301
                     /locus_tag="ACIUS0_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGB0218705.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40341..40862)
                     /gene="fldB"
                     /locus_tag="ACIUS0_000836"
     CDS             complement(40341..40862)
                     /gene="fldB"
                     /locus_tag="ACIUS0_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGB0218706.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40974..41870
                     /gene="xerD"
                     /locus_tag="ACIUS0_000837"
     CDS             40974..41870
                     /gene="xerD"
                     /locus_tag="ACIUS0_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGB0218707.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41895..42605
                     /gene="dsbC"
                     /locus_tag="ACIUS0_000838"
     CDS             41895..42605
                     /gene="dsbC"
                     /locus_tag="ACIUS0_000838"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGB0218708.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42611..44344
                     /gene="recJ"
                     /locus_tag="ACIUS0_000839"
     CDS             42611..44344
                     /gene="recJ"
                     /locus_tag="ACIUS0_000839"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGB0218709.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44435..45533
                     /gene="prfB"
                     /locus_tag="ACIUS0_000840"
     CDS             join(44435..44509,44511..45533)
                     /gene="prfB"
                     /locus_tag="ACIUS0_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGB0218710.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45543..47060
                     /gene="lysS"
                     /locus_tag="ACIUS0_000841"
     CDS             45543..47060
                     /gene="lysS"
                     /locus_tag="ACIUS0_000841"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGB0218711.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47103..47651)
                     /gene="idi"
                     /locus_tag="ACIUS0_000842"
     CDS             complement(47103..47651)
                     /gene="idi"
                     /locus_tag="ACIUS0_000842"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGB0218712.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47706..47777
                     /locus_tag="ACIUS0_000843"
     CDS             47706..47777
                     /locus_tag="ACIUS0_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218713.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47774..47899)
                     /gene="yqfG"
                     /locus_tag="ACIUS0_000844"
     CDS             complement(47774..47899)
                     /gene="yqfG"
                     /locus_tag="ACIUS0_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGB0218714.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47901..49349)
                     /gene="uacT"
                     /locus_tag="ACIUS0_000845"
     CDS             complement(47901..49349)
                     /gene="uacT"
                     /locus_tag="ACIUS0_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGB0218715.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12055 a        12450 c        13405 g        11921 t
ORIGIN      
        1 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
       61 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
      121 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
      181 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
      241 gttaccaagc ggatattcag cgggctacag ctacgaatat tttggcggca atgccactta
      301 tatgattatt cccgaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
      361 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa
      421 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
      481 tgcactgctg gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg
      541 tggcatacaa ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt
      601 gcagaagctg ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa
      661 taccaaaggg atgagcgatc ctatccagat gctgcgggcg ctgacaggag atgtcgggtt
      721 cgatgacatt tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact
      781 ggcggaagat ggctgtctga acttctttgc cgggctgacg gataaaaact tcaaagtgcc
      841 gtttaatttc tacaacgtcc attacaacag cacgcacgta gtcggtacat ccggcggttc
      901 aacggacgac atgaaagagg cgattgccct tagcgccaca ggacagttac agccgtcgtt
      961 tatggtgacc catattggtg gcctggatgc ggtgccagaa accgtgctca atctgccgga
     1021 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     1081 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
     1141 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     1201 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     1261 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     1321 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     1381 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     1441 ggcgatgggc cagaagtcga tatcgcggtt gatcccattg aaggcacgcg gatggtggcg
     1501 atggggcaga gcaatgcgct ggcggtcatg gctttcgctc ctcgagatag cctgttacat
     1561 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     1621 ctgtctttgc ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taaaccgctg
     1681 gacaagctgc gcatggttac gctggataaa ccgcgtctga gtgccgcgat tgaagaagca
     1741 acacagctgg gcgtgaaggt ttttgccctg ccggatggcg atgtcgccgc cagcgtgctg
     1801 acctgctggc aggataatcc ttacgatgtg atgtacacca tcggcggtgc gccagaaggc
     1861 gtgatttctg cctgtgccgt taaagcgtta ggcggggata tgcaggccga gctgattgac
     1921 ttttgtcagg ctaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
     1981 cgttgtaagg caatgggcgt tgacgttaac cgtgtgtact cgctcgatga actggtgagg
     2041 gggaacgata tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc
     2101 caacagacgg cgaatggggt gcggacgcag acattactga tcggcggcgc ggaccaaacg
     2161 tgtaatataa tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg
     2221 acagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
     2281 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     2341 aacgatgaag agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     2401 gacgatattg atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     2461 tatgccgata tcacgcactt ttcccagtac tggcattacc tgaacgaaca ggatgaaacg
     2521 cccggatttg ccgatgatat aacctgggat tttatcagca atgtcaacag tataactcgc
     2581 aatgcgacgc tctacgatgc gttaaaagcg atgaagttcg ccgatttcgc cgtctggtca
     2641 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
     2701 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
     2761 accatgatga agagatagaa cgcgtgcata aactgttgct gcgtatgctg gcggccttac
     2821 agacggtaaa tccgcagcgg cgtacggtgg tttttctttg cgctccgccg gggacgggta
     2881 aatctaccct aaccaccttc tgggaatatc tcgcgcaaca agacccggag ctgcccgcta
     2941 ttcaggcgct cccgatggat ggttttcacc attacaatag ctggctggac gcgcatcaat
     3001 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgc
     3061 gccaggtggt ggaaggggat tgtacgtggc cgcagtacga tcgacaaaag catgatcctg
     3121 ttgaagatgc gttgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc
     3181 tggacgacga gaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaacg
     3241 cgccagctgc tgcgttgcgg gagcgtctgg tggggcgcaa gctggcggga ggggtatcgc
     3301 tggcagatgc agaagccttt tatgaccgta ccgacgggcc gaatgttcgc cgggggctgg
     3361 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
     3421 tggattaacg ttcctgatta caccatcttc cagccgcacc acacgagaga aatgacgccg
     3481 ggtaaatgcg gcgtcatgac tgattgctac cacgctcgtg cctcgctggc ggcacttctc
     3541 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
     3601 cagtaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
     3661 attatgcaga tccaacgggt gcgagttggc agtgtctgct aactgacaac attgcagggc
     3721 agcatgggta cgttgagtga tttcatccac cgggcatttt tgcagtttca aaccaaatgc
     3781 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
     3841 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     3901 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg atttccctgc
     3961 gccattgtca ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     4021 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     4081 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     4141 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     4201 tgcaacagca tttcgctggc ctgcggtgtc aggcggctaa aggcttcatc aagaattaag
     4261 agctttggct gcatcgcgat ggcgcaggcg atgatcacgc gctgtgtctc tccaccggag
     4321 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     4381 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     4441 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     4501 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     4561 gtgcctgtta tctctcttgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     4621 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     4681 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     4741 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     4801 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     4861 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     4921 gcggaataat taacgcgggc atagcacgta aacgctgata ccagccctca tccagcggca
     4981 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     5041 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     5101 cgcgaataaa acattgcacg gggacgtact gcattcacaa ctgtgaggtt gaaacaatcg
     5161 ccagaatcag caaccagagc gtaatagcat gcgaccaccg ttctggcgag cgtggcgtgc
     5221 cgcttagcca ttctggtagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     5281 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaga
     5341 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     5401 aggccgctaa tgcccacagt gttaatgagg taaatggatg catttagcgc acggcagcca
     5461 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     5521 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     5581 ccaggttttg ccccatcgag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     5641 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     5701 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     5761 cggagaccag gccgatgacc atcgcgacgg gtgcaaatgc ggcggcgatc gggtcggtca
     5821 gcaatcccca gagtaaattg gtcagtaagc cggtcagcat tccgatcacc ggaccaagca
     5881 gtaccgcgct aataagtgtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     5941 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     6001 tggaaaagtg gctacgcgcc ataagaaaac ctctttgaaa gtagttattt aacgggggaa
     6061 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     6121 aggctaccga tcaccagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     6181 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     6241 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     6301 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ctaggcggca
     6361 tgaagttgat gttgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagg
     6421 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgagt agggcaatgt catcattgga
     6481 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     6541 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     6601 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agataattgc
     6661 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     6721 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     6781 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     6841 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     6901 tcgtgctttg tatgaatccg gacgccgggc ggaaattacc gtggtggcaa tcaacgaact
     6961 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagccatg gccgttttgc
     7021 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     7081 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     7141 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     7201 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     7261 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     7321 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     7381 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     7441 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgctgg
     7501 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     7561 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     7621 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     7681 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     7741 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     7801 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     7861 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     7921 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
     7981 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
     8041 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
     8101 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
     8161 aagacaaact gtctaacccg gttcgtctgg ttaaagatta cctcgacggc gttgacgttg
     8221 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
     8281 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
     8341 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
     8401 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
     8461 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
     8521 actccctgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
     8581 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
     8641 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
     8701 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
     8761 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
     8821 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
     8881 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
     8941 acactctggc agcaatcgac ctgttcggca ttgctgacaa aatctcctac atctccactg
     9001 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
     9061 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcacttg tttcagcgcg
     9121 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
     9181 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
     9241 acatgcaact tgaagtatta tgagtataag gcccgacgat acaggacaag agacatgtct
     9301 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
     9361 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
     9421 atcaacgccg tactggaaac tgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
     9481 aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggtgct
     9541 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
     9601 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
     9661 ctgctggacg cgggtgaaaa acacttcgca gctaccggta agccgctgtt ctcttctcac
     9721 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
     9781 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
     9841 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
     9901 gttgattacg catacaccga gctgagcaaa atcagcccgc gtttcaccat cgctgcgtcc
     9961 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    10021 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    10081 gtattccacg gtggttccgg ttctactgct caggaaatca aagactccgt aagctacggc
    10141 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    10201 tactacaaag cgaacgaagc ttatctgcag ggtcagctgg gtaacccgaa aggcgaagat
    10261 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    10321 atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagtcatt
    10381 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaggtgat
    10441 tcagatgaga aatctgtgat ctatttggca aaattatgct ttattgttta cccttgtcag
    10501 actgcccgtc ataaggcggc ggagtgtatt tctccatttt gagtcagttg aaaaggaata
    10561 ttgaatggaa gatttgaatg ttgtcgatag cataaacggc gcgggaagct ggctggtagc
    10621 taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg gcggcactcg cgatcatcat
    10681 cgttggtttg attatcgcgc ggatgatttc caacgcggtg aatcgcctga tgatctcccg
    10741 taaaatcgat gccactgttg ctgattttct ttctgcatta gtccgttacg gtattatcgc
    10801 ctttacgcta atcgctgccc tgggacgcgt gggtgtacaa acagcgtcag tcattgctgt
    10861 actcggtgcc gcaggcttag ctgttggtct ggctttgcag gggtcacttt ctaacctggc
    10921 cgctggcgtg ttacttgtca tgttccgccc gttccgtgcc ggagaatatg ttgacctcgg
    10981 cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc accaccatgc gtactgcaga
    11041 cggtaaaatt atcgttattc cgaacggtaa aattattgcc ggaaatatta ttaacttctc
    11101 ccgcgagcca gttcgccgta acgaatttat tattggcgtg gcgtatgatt ccgatatcga
    11161 tcaggttaag cagatcctga ccaatattat ccagtctgaa gatcgcattt tgaaagatcg
    11221 cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg attaatttcg tggtccgcgt
    11281 ctggagcaac agcggcgatc tgcaaaacgt gtactgggat gtgctggagc gtattaaacg
    11341 tgaatttgat gccgccggta tcagcttccc gtacccgcaa atggatgtga actttaagcg
    11401 ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca gataatactc tctggcctct
    11461 ctcttattag ttattctgat tgccaattaa tattatcaat ttccgctaat aacaatcccg
    11521 cgatatagtc tctgcatcag atacttaatt cggaatatcc aacgtgtttt cttattactt
    11581 tcaaggtctt gctcttgggg cagctatgat tctgccgctc ggcccacaaa atgcttttgt
    11641 gatgaatcag ggcattcgtc gtcagtacca cattatgatt gccttacttt gcgctatcag
    11701 cgatttggtc ctgatttgcg cggggatttt tggtggtagc gcgttattga tgcagtcgcc
    11761 gtggttgcta gcgctggtca cctggggcgg cgtagtcttc ttgctctggt atggttttgg
    11821 cgcttttaaa acagcgatga gtagtaatat tgagttagct agtgccgaag tcctgaagca
    11881 aggcagatgg aaaattatcg ccaccatgtt ggcagtgacc tggctgaatc cgcatgttta
    11941 cctggatact tttgttgtac tgggcagcct tggcgggcag cttgacgtcg aaccaaaacg
    12001 ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg ttctttggtc tggctattct
    12061 cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca cagcgcatta tcaatctggt
    12121 agtgggatgt gtcatgtggt ttatagcctt gcagctggcg agagacggta ttgctcatgc
    12181 acaagccttg ttcagttagg cgctatctga tggaaaaata aaacagaggc gctaagcttg
    12241 cctccagagg tcctgaattt tgggcaagtc aattaacacg gagagactaa cgtgaagttc
    12301 aaagttatcg ccctggcggc attaatgggt attagcggga tggcagcgca ggctaacgaa
    12361 ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa gtgtggatgc ggtgccagac
    12421 attgccactc ttgcgattga agttaacgtg gccgcgaagg atgccgctac agccaagaaa
    12481 caggcagatg agcgcgtcgc acaatacatt tccttccttg aactcaatca gatcgcgaaa
    12541 aaagatatca gctcagcgaa tttacgcacc cagccagatt atgactatca ggatggtaaa
    12601 agtatcctga aaggctaccg agctgtgaga acggtggaag tcacgctccg tcagttagac
    12661 aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc ttaacgaaat tcgttctgtg
    12721 tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag cgcgtaaggc agcgattgat
    12781 aacgcgattc atcaggcgca ggaactggcg aacggctttc atcgtaaact ggggccggta
    12841 tatagcgtgc gctaccatgt ttccaactat cagcccagcc caatggtgcg gatgatgaaa
    12901 gccgatgccg cgccggtgtc tgcccaggaa acttacgagc aggccgctat tcagtttgat
    12961 gatcaggtcg atgtggtctt ccagttagaa cctgtggatc aacaacccgc taaaacacct
    13021 gcagcacaat aatacttgta gcccgacgta ttcgtcgggc taatgccttt acccgatatt
    13081 gcctcctttt tctgtaattt tctgtaaaac catgacgtaa tcacctatct ttgctcgcgg
    13141 tttcatcaag gataggctga ctgaaacggt taagcggaca aggatgcggg gaggggggat
    13201 ggacattttt atttcgaaaa agatgcgcaa tttcatttta ttagcgcaaa ccaataatat
    13261 tgcccgggcg gcagaaaaaa tccatatgac ggcttcgcca tttggtaaaa gcattgccgc
    13321 gctggaagaa caaattggct atacgctatt tacccgcaaa gataataata tcagcctcaa
    13381 taaagccggt caggaactgt atcaaaaact gttcccggtt tatcaacgac tttctgcgat
    13441 tgataatgaa atccataact cagggcgtcg ttcacgagaa attgtgattg gcatagataa
    13501 tacttatccg acaattattt tcgatcagtt gattagtctc ggagataagt atgaaggcgt
    13561 gaccgcccag ccagttgagt tcagcgaaaa cggcgttatc gataatcttt ttgaccgtca
    13621 gttagatttt attatctccc cacagcatgt atctgcacga gttcaggaac tggaaaacct
    13681 gactatcagt gaattaccac cgctgcgatt gggctttctc gtttctcgcc gttatgaaga
    13741 gcgtcaagag caagagctgc ttcaggagct gccctggcta caaatgcgct ttcaaaaccg
    13801 ggctaacttc gaagcaatga ttgatgcgaa tatgcgacct tgtgggatca atcccaccat
    13861 tatttatcgt ccctacagtt ttatggccaa aatcagcgcc gtggagcgcg gacattttct
    13921 gacggtgatc ccgcattttg cctggcgact ggtgaatccg gcaacgctga aatattttga
    13981 tgcgccgcac aggccgatgt atatgcagga atatctttat tcaatcagaa atcatcgtta
    14041 taccgccacg atcttcagca tattgctgaa gatcgtgacg ggacaaacca ttaacccagc
    14101 atcgagccgg ttgcaattaa attacggtgt aagtcgaaga cgtggctaag atcgtggtga
    14161 atatgtccgc cgggcgcatt ttccagatag cgatgcagat aatcccgata cataggatgt
    14221 gcacaattat caatgatagt gcgggcgcgt tgaagcgggg aaagaccgcg cagatcggcg
    14281 atcccttgtt cggtgatgat cactttgacg ctgtgttcgc tgtgatcaac atggctgcac
    14341 attggcacga cggttgagat cttgccttct ttagcaatcg acggagccat aaagatcgac
    14401 agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc cgttcatcag atcgaccccg
    14461 gctacgtgcg ttgaatttgc atgcccgtaa atatcaaact ccaggccgac gttcagagcg
    14521 atgacgccca gacgacggat gatttccggg ttattggaaa tctcctgcgg acgcaacaca
    14581 atgcggctgg caaagtaatc catattgtcg taaatcttgc gcagggaatc ggccgagatt
    14641 gtcaggctgg aggcgctggc cccgctgatt ttgccggttt ccagtaaatg caccaccgat
    14701 tcctgtagca cttccgaata catcatgaac ggaggaattt ccgggttttc ccccagacgt
    14761 gccattaccg cattattgat attgcccacg ccactttgca gcggcagaaa ttccggcgga
    14821 atacgcccat gcgccatttc ctgcaataag aacgtgacca cgttatcggc aatctgctgg
    14881 cacatgggat tttgcttatc cagcatatta ccggcgtcgg gcaagttggt ttccacgacg
    14941 gcgacaatct ttttcggatc gatttgcaca tagcgggtac cgacgcgatc cattgcatgg
    15001 aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa tcacaatatc tgccagttct
    15061 gcaacgcgcg gatcgtgata gtgattaagt tcaatgatca ctttcttcgc tcgcagcagc
    15121 taggtcggcg cattaccgat cccgctggtt aaccagactc gaccatccgg tgccagtgcc
    15181 gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac cgtaattgac catttgcgcc
    15241 acttcgctca aatgcaggtc aacgaaactc accgcgccct gattgatctt tttacgtaaa
    15301 ctggacgatg tttgatatgg cgcacgccag gaaacagcat cggcgtcaga aagtacatcg
    15361 tcagcggcgg cgctgattga cgcccccgtc agcaggcgaa tttgatacgg ctttttggcc
    15421 tcatgctgtt cgttagctct gcgggcaatc gcggcgggta gggctttcgg cgaacccgct
    15481 ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga taatttctgc cgcttcatcg
    15541 gcggtcatcc ttgtccactg agtttccatt tttactcctt cgttcgcagg gattaatgac
    15601 caacgaaatt aggtttacgt ttttcgagga aagcgttcat cccttcctgg taatcttcgc
    15661 tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa ttcatcggag ttcatggtgt
    15721 gtgcttcgcc cagtacacgc agctcttctt tgataacggc aatggctaac ggcgctttct
    15781 cagagatgtg gtgcgccatt tgtaaggtga aatcttccag ttcttccact tccacaacat
    15841 ggttgaggat gccgacagcc agcgcgcgct gggcggtgat tggcgaagcg gtaaaaatca
    15901 gctctttgac aatgtggaag cccgcgtcgc gggtcaggtt gtgaatgccg accaggttat
    15961 acgggacgcc gaggtttaca ggcgtcattg agaaggttga ggtactggcg gcgatgatca
    16021 gatcggaact catgatcatt tcaaatgcgc caccccaaac actaccttcc accatcgaaa
    16081 tgatcggttt cgggaatttt tggatcatgc gggtgatttg acgcaatgga tcatcatagg
    16141 agagcggatc gcgaccgcca gacggcagtt cgtgaatatc gtgacctgcg gagaagactt
    16201 tggatccact tggcgcccgc aaaatgatac agcgaatttc cggccggttg agatcgctta
    16261 acgcctgcat aagatcatca ataaagactt tacttaaggc attaagtttt cgtccatagt
    16321 taaactcaat gaccgccact ttgttgatag tggcaacgtt cacatactga taagacataa
    16381 aaattccttt aatcaaaata ttgcgtctgg ataaattcac tgagctgccg caggccggtg
    16441 cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct ggcggtaata gcgatcgaaa
    16501 tcttcattcg cgaacaggtg attcagtact tcttcttcgg tctgcttacg cagccattcc
    16561 accgattgtt gttgccgcac ttgttgtaaa cgaccactgg cagttagcgc ggttttgaag
    16621 tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt ccagtgcgct acaagtcaga
    16681 ccccgtggct gccattcgtc gtatttacgt cgcagaatat gcagggcact ctcgtacata
    16741 tgccgggcaa tggcgacatt ggtatggtta tcgccatcgt ctttgttgat aacgagcaga
    16801 tcagccactt ccatcagccc ttttttaatg ccctgcagat catcgccgcc accggcaatt
    16861 tgcaacgaga taaaacagtc caccatgcgg gcgacttccg tttccgactg cgcgatgcca
    16921 accgtttcga caatcactac gtcataaccc gccgcttcgc ataacagcat taattcccgt
    16981 gctcgctgac tggcaccgcc cagatgaccg gaggagggta ccgggcgaat aaacgccgcc
    17041 tcggcacgcg ccaggtcatt catgcgggtt ttatccccga gaatgctacc gccagtgacc
    17101 gggctgctgg gatcgaccgc aataacagca acctttaatc cctcacgaat caacaacatg
    17161 ccaaaggctt caagaaaggt acttttcccc gcgccgggtg tgccggtaac gcccagtcgc
    17221 agggcgttac cacagaacgg cataatagcg tcaagcagct gtgtacttag cgcctgatga
    17281 cgtgggtgac ggctttccac cagcgtcatg gcttgggcga gcgtggcacg ctcaccctga
    17341 cgtaagcggc gaatactttc tgccagcgtg gcttcattaa tcatgatgct ggcttatcag
    17401 attcagtacg tcgcgcacac tgtcgagcat aggtgtacct ggaccataaa tcgccgccac
    17461 gccgcgctct tgcaggaagg cgtaatcctg cggcggaatg acgccacccg cgaccacgca
    17521 gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt tccgggatca gcgttttatg
    17581 accggcagcc agtgaggatg cgcccactac gtgaacgtcg ttttctacgg ctaggcgggc
    17641 gatctcttca ggtgtagaga acatcgggct taaatctacg tcgaaaccga gatcggaata
    17701 ggcgctggcg atcactttcg cgccgcgatc gtgtccatcc tggcccatct tagcgatcag
    17761 aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc gtttgcgcaa caatggcatc
    17821 gaactcggag gccgatttct cagactgatg atagctttgc gcaatcacgc cggtaacaca
    17881 ctggcttggc accagataac ggtcgaaagc gacttccagc gcatcggaaa tttcacccag
    17941 ggtggcgcga acgcgagcgg cattaacagc ggcagccagc aggttttcgt tatgctgtgc
    18001 ggcgtgagtc agggcgttca acgcggcggt tacggcggca tcatcacggg tggcgcgaat
    18061 gcgttccagc gaagcaattt gctcgttacg caccatcacg ttgtcgatct caagtacatc
    18121 ggtttcgtct tcgtgatcca gtttgtactt gttgacacca acgatgacac gcttgccctg
    18181 gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc attcgttttg gcagacctgc
    18241 ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt tggataatag ctctggcttg
    18301 tttgacgatt tgatcggtca gcgactcaat gtaataggat ccggccagtg gatcgacggt
    18361 gtggcagagt tctgattctt cctggatgat gatctgggtg ttgcgggcaa tgcgtgctga
    18421 gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg gtatgcagtg actgagtacc
    18481 gcccagcgtc gcagccagtg cttcaatggt ggtgcggata acgttgttat acggatcctg
    18541 ttcagtcagg ctccagcctg aggtctggca gtgggtacgc agcgccagtg atttcggatc
    18601 ctgtgcgcca aatccactga ccgcttcgct ccataaataa cgtgccgcac gcaacatggc
    18661 gacgttcata aacagatcca tgccgatacc gaagaataac gacaggcgag gagcgaagtc
    18721 atcaattttc agtccggcag agattgctgc tttgatgtac tcaatcccat cggcgagcgt
    18781 aaatgctacc tgctgcacgc agttggcacc cgcttccccc atgtggtaac cgctgatact
    18841 gatggtatta aatcgcggca tgttgccgga acaccaggcg atgatgtcgg cgataatgcg
    18901 cattgacggt tttggtgggt aaatataggt gttgcggcag aggtactctt tgagaatatc
    18961 gttttgaatg gtgccggtca gtttatcagg tgtaacacct tgctcttctg cggcgacgat
    19021 ataaaacgcc agtactggta gcactgcgcc attcatggtc atcgaaaccg acattttatc
    19081 cagcgggatc tggtcgaaca ggactttcat atcttccacg gtgtcgatag cgacgcccgc
    19141 tttgccgacg tcacccgcca cgcgtgggtt atcggagtcg tagccacggt gggtggcaag
    19201 gtcaaacgca acggaaagac ctttttgccc ggcggccagg ttacggcgat aaaaagcgtt
    19261 ggactctttt gctgttgaaa aaccagcata ctgacggatg gtccacggtt gggcggtata
    19321 catagtggca cgcgggccac gaacgtaggg cggcaaacca ggaagggtac ctgtcacctc
    19381 cagattatcg agatcggctt cggtatacag cggcttgatg gcgatccctt ccgcggtttg
    19441 ctgaaccagc gagtcgacag ttttctcccg acggctcaat tccttgttgg caagctgttg
    19501 ccactcctgc tcgttagaca ttatttgctc cgtaaaacgc aatcaaaatg agggtatttg
    19561 cgcttacggt gagcttttta cgtgggtttg tcgccagcaa aaaaaatgca ttcagacgtc
    19621 gttttttgcc gcctgagatt tgtctatttt gtgatgtgat tcaaggaata caaaaagaag
    19681 tgaggacaac gcctgatatg tgctatcagg cattatttga tggattaatc ctgacgaagg
    19741 actttgtgac cataatcgag taacgcatca gtgactttac gcatcatgcg gctttcagga
    19801 gcaaagcggt gccagtagag catccgtcgt tgaaatagcc caggcgttaa gtcaatcagt
    19861 tcaccgctgg ccagctcttt ctcgatttgc aggtgcggga tcatacagca ggtggtgccc
    19921 tggcgagcaa gttgtacgaa cgcttctgaa gaattaacga tatggcaggg tacgctgcca
    19981 ggaggaagat cgaagttttg ctgcaaaaag gcctggtgca tatcgtcaag atggtcaaac
    20041 gcgaccactg gcgctttcaa taatgccgaa cgcgttacgc cgttaggaaa atatttttcg
    20101 gcaaacggtt ttgagctgac gaacagatag tcgagcgcac cgagtttatc gacaagacaa
    20161 ctcggcagcg cctgatgttg aatactcacc gcgccgacca cttcgccgcg gcgcagacgt
    20221 tcctgagtac gggtttcatc ttctacctgc aagttgaggc ggataggcga atcagccaac
    20281 acaggagcca gtgccggaag caaccacgtc gccagactgt cggcgttgac cgccagtgaa
    20341 agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc actcttcttc cagcaactcc
    20401 acctggcgca gcagtgccag cagtttttgc ccttgttccg tcgggcgcgg cggtacggta
    20461 cgcaccaaca gcggctgccc gaacatattt tccagttgct taatgcgctg tgagacggct
    20521 gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc ctcgttcacg tatcaccgca
    20581 tccagtgcct gtaatgttct gtagtccggg cgtttcattg ctgttgtctg ctcctggtaa
    20641 atgctttttc ctgcactatg acacaatttt gtgtcaggtt gcatataccg aagccgtcgg
    20701 cgggtaacat ttgtatcaat ggttaaacgc gcagcttaaa agatcaggga tttgcgctat
    20761 tgcatttcgc ttagcgactt cgcgccctgg ttatgttgaa tggcgtggcg ttattgcctc
    20821 tgttcgcacc gtggagcggc atacccagac cgttgtatgc tttatttgcg gggcgaaagg
    20881 ggatgcccgc cattgcgcga tattttatgg atgagttaac cacccggctt gccaacgggg
    20941 tctgaatcgc tttttttgta tataatgcgt gtgaaatttc ataccacagg cgaaacgatc
    21001 atgacgcagg atgaattgaa aaaagcagta ggatgggcgg cacttcagta tgttcagccc
    21061 ggtaccattg ttggtgtagg tacaggttcc accgccgcac actttattga cgcgctcggc
    21121 acgatgaaag gccagattga aggggccgtt tccagttcag atgcttccac tgaaaaactg
    21181 aaaagcctcg gcattcacgt ttttgatctc aacgaagtcg acagccttgg catctacgtt
    21241 gatggcgcag atgaaatcaa cggccacatg caaatgatca aaggcggcgg cgcggcgctg
    21301 acccgtgaaa aaatcattgc ttcggttgcg gaaaaattta tctgtattgc cgacgcctcc
    21361 aagcaggttg atattctggg taaattcccg ctgccagtag aagttatccc gatggcacgt
    21421 agtgcagtgg cgcgccagct ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg
    21481 gtgaccgata atggcaacgt gatcctcgac gtccacggca tggaaatcct tgacccgata
    21541 gcgatggaaa acgccataaa tgcgattcct ggcgtggtga ctgttggctt gtttgctaac
    21601 cgtggcgcgg acgttgcgct gattggcaca cctgacggtg tcaaaaccat tgtgaaatga
    21661 tctgacgggg gaacctcccc cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt
    21721 cacgctttta ccaggcaatt gtcgattgct ctaaataaat cctctaaacc agcatattca
    21781 tccaagaatt acctttgcgt gatatttcct caacatcgcg acgcaaacgt tcatattgcc
    21841 gcaatattat tttttgatat gttgaaaggc ggatgcaaat ccgcacacaa catttcaaaa
    21901 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
    21961 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
    22021 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
    22081 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaaa
    22141 ctggtcgcta ttggctgttt ctgtatcgga acaaaccagg ttgatctgga tgcggcggca
    22201 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
    22261 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
    22321 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
    22381 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
    22441 tatgtttact tttatgatat tgaaaacaaa ctgccgctgg gcaacgccac tcaggtacag
    22501 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
    22561 tccaccaaaa atatgatggg cgcgaaagag atttcgctaa tgaagcccgg ctcgctgctg
    22621 attaatgcct cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
    22681 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
    22741 ccatttacct ctccactgtg tgaattcgac aacgtccttc tgacgccaca cattggcggt
    22801 tcgactcagg aagcgcagga gaatatcggc ttggaagttg cgggtaaatt gatcaagtat
    22861 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
    22921 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
    22981 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
    23041 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
    23101 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
    23161 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    23221 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    23281 cgttgtgttt atttaatggg gatttacgat ggcgggacag catggggctg taacggtaaa
    23341 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    23401 attgtactgt tactgataag tttcggagct tattaacagt cagtctcaag ggaggagtca
    23461 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    23521 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    23581 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgagcac
    23641 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    23701 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    23761 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    23821 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    23881 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    23941 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    24001 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    24061 ttgtaaggtc cgatcataaa aaccaccgcc cattcccaga cgctgaccgt actcatcaaa
    24121 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    24181 cagttttggc tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    24241 caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    24301 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    24361 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    24421 ttgacccatt tccagctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    24481 gcggatttct tgtcgggata atgtcagtgg gagctctggt agttgcgtca tggctggtgc
    24541 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    24601 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg aaccgagcat gctcaccaac
    24661 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    24721 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    24781 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    24841 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtctttgct
    24901 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    24961 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    25021 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    25081 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    25141 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    25201 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    25261 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    25321 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    25381 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    25441 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    25501 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    25561 cagcgttttc gatcgggctg atgcactggc aggttgggtc aatcacttcc tgcttggtct
    25621 tggcgttacg caaccgaagc tggacaaagt gaccggcgaa accggtgaag ccatcgacga
    25681 tctgcgtaac atcgcgcagc tgggctacga cgaagacgaa gatcaggaag agcttgaaat
    25741 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    25801 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    25861 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    25921 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    25981 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    26041 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    26101 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    26161 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    26221 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    26281 atgcatatgc tgatgaaatc gtgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    26341 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    26401 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    26461 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    26521 aaattcacca cgaatttaac cgccacggtg cgcgctatcc ttcctataac accattgtag
    26581 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    26641 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    26701 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    26761 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    26821 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggta
    26881 atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc
    26941 actggttagg actggatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    27001 tggaaccggg gatggtactg accgtagagc ctggtctgta tatcgcgccg gatgcggatg
    27061 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    27121 ccggcaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    27181 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    27241 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    27301 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    27361 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggctg attgcgcaac
    27421 cgccatcact accgtgcatg tcagcgatcg tggtcatgct ggattcgtca ccctcgccgc
    27481 agaagattac caactggcgg cgctggggca ggttgtcgaa ttgcataatg tcgggcaacg
    27541 gctgtttgca ttgctgcgaa aagcacctgg cgtaatgctg cattgccccg atcgcgtagc
    27601 taacgttgcc cgtactcaga gtcatgttga agtgacgctg gagagcggcg agatgctgac
    27661 gggccgcgtg ctgatagccg ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    27721 tgattggcag caggagcctt acgaacaact ggcagtgatt gccaacgttg ctacttctgt
    27781 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    27841 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    27901 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tctgcctttg gctggcgact
    27961 ggggcaaatt acccacgccg gtaaacgcag tgcttatccg ctggcattaa cccgcgccgc
    28021 cagaccgatt acccatcgca ctgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    28081 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    28141 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    28201 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    28261 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    28321 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    28381 gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg
    28441 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    28501 agcgcgtacc ggaacctctg gcggtagatg cactaccaca actgcgcgtt tcggctatca
    28561 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctcgctcgta
    28621 gggccagttg ttatcacggc atggaagtgt gggacaaaga cagttttggt cacatctcgt
    28681 ttgacgatca aagcatgggc tatagccatc ttgggcacat cgttgaaaac tcagtgattc
    28741 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat tactctgttg gcccccacag
    28801 aattacagca ggtcgcctgg ggagaaaatg aaaccttcct gacgctgaaa gatggcagca
    28861 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    28921 cagatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    28981 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    29041 ttttaccgct tagcgatccg catctttgct cgattgtctg gtcactgtcg ccagaggaag
    29101 cgcagcggat gcagcaggca agtgaagacg aatttaatcg cgcgttaaat atcgcttttg
    29161 ataatcgcct tggcttatgc aaggttgaga gcgcgcgtca ggtgttcccg ctgacggggc
    29221 gttatgcgcg ccagtttgcc gcacaccgtc tggcgctggt gggcgacgcc gcgcatacca
    29281 ttcacccact ggcggggcag ggcgttaatc tcggatttat ggacgccgca gagctgattg
    29341 ctgaactgaa acggttgcat cgtcagggta aagacatcgg gcagtacatt tacctgcgcc
    29401 gctatgaacg tagccgcaag cacagcgcgg cgctgatgct ggctggtatg cagggttttc
    29461 gcgatctctt ttccggcgct aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    29521 tggccgacac gcttcctggc gttaagccgc aacttatccg ccaggcaatg ggattaaacg
    29581 atttgcctga atggctgcgt taaaaatttc tcctctgttg tttatttgat acccatcaca
    29641 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    29701 ctccgttttc gcattatatt ttctaatacc atgatttttt gatttagtgt tttttgacat
    29761 ttttttagct cttaatattg tcttattcaa attgactttc tcatcacatc atctttgtat
    29821 agaaattggt gtattttttg gttttttatt ctgtcgcgat ttttgcattt tttaaccata
    29881 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    29941 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt tttgtggtga
    30001 taatttcacc atgaaaaagt tgtcagcccc gcttattcaa tgaggacaag atggcacaac
    30061 agactccttt gtacgaacaa cacacgcttt gcggcgctcg catggtggat ttccacggct
    30121 ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca tcatgcggta cgtaccgatg
    30181 ccggaatgtt tgatgtgtcg catatgacca tcgtcgatct ccgcggcagc cgcacccggg
    30241 agtttctgcg ttatctgctg gcgaacgatg tggcgaagct caccaaaagc ggcaaagccc
    30301 tttactcggg gatgttgaat gcctctggcg gtgtgataga tgatctcatc gtctactact
    30361 ttactgaaga tttcttccgc ctcgttgtta actccgccac ccgcgaaaaa gacctctcct
    30421 ggattaccca acacgctgaa cctttcggca tcgaaattac cgttcgtgat gacctttcca
    30481 tgattgccgt gcaagggccg aatgctcagg caaaagctgc cacactgttt aatgatgctc
    30541 agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt gcaggtgggc gatctgttta
    30601 ttgccaccac tggttatacc ggtgaagcgg gctatgaaat tgcgctgccc aatgaaaaag
    30661 cggccgattt ctggcgtgcg ctggtggaag cgggcgttaa gccctgcggt ctgggggcgc
    30721 gtgacacgct gcgtctggaa gcgggcatga atctttatgg tcaggagatg gacgaaacta
    30781 tttctccttt agccgccaac atgggctgga ctatcgcctg ggaaccgaca gatcgtgact
    30841 ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg tacagaaaaa ctggttggtc
    30901 tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc ggtacgcttt accgatgcgc
    30961 agggcaacca gcatgaaggc attatcacca gcggtacttt ctccccgacg ctgggttaca
    31021 gcattgcgct ggcgcgcgtg ccggaaggta ttggcgaaac ggcgattgtg caaattcgca
    31081 accgtgaaat gccggttaaa gtgacaaaac ctgtttttgt gcgtaacggc aaagccgtcg
    31141 cgtgatttac ttttttggag attgattgat gagcaacgta ccagcagaac tgaaatacag
    31201 caaagaacac gaatggctgc gtaaagaagc cgacggcact tacaccgttg gtattaccga
    31261 acatgctcag gagctgttag gcgatatggt gtttgttgac ctgccggaag tgggcgcaac
    31321 ggttagcgcg ggcgatgact gcgcggttgc cgaatcggta aaagcggcgt cagacattta
    31381 tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca ctgagcgatt ccccggaact
    31441 ggtgaacagc gaaccgtatg caggcggctg gatctttaaa atcaaagcca gcgatgaaag
    31501 cgaactggaa tcactgctgg atgcgaccgc atacgaagca ttgttagaag atgagtaacg
    31561 gctttattcc tcttctgcgg gagaggatca gggtgaggaa aatttatgcc tcaccctcac
    31621 tctcttcgta aggagagagg ttcacaattc actgcacgtt tcaggaacca tcgctcatga
    31681 cacagacgtt aagccagctt gaaaacagcg gcgcttttat tgaacgccat atcggaccgg
    31741 acgccgcgca acagcaagaa atgctgaatg ccgttggtgc acaatcgtta aacgcgctga
    31801 ccggccagat tgtgccgaaa gatattcagc ttgcgacacc accgcaggtt ggcgcaccgg
    31861 cgaccgaata cgccgcactg gcagaactca aggctattgc cagtcgcaat aaacgcttca
    31921 cgtcttacat cggcatgggt tacaccgccg tgcagctacc gccggttatc ctgcgtaaca
    31981 tgctggaaaa tccgggctgg tataccgcgt acactccgta tcagcctgaa gtctcccagg
    32041 gccgccttga agcactgctc aacttccagc aggtaacgct ggatttgact ggacaggata
    32101 tggcctctgc ttctcttctg gacgaggcca ccgctgccgc cgaagcaatg gcgatggcga
    32161 aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt ggcttccaat gtgcatccgc
    32221 aaacgctgga tgtggtccgt actcgtgctg aaacctttgg ttttgaagtg attgtcgatg
    32281 acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt gctgttacag caggtaggca
    32341 ctaccggtga aattcacgac tacactgcgc ttattagcga actgaaatca cgcaaaattg
    32401 tggtcagcgt tgccgccgat attatggcgc tggtgctgtt aactgcgccg ggtaaacagg
    32461 gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt gccgatgggc tacggtggcc
    32521 cacacgcggc attctttgcg gcgaaagatg aatacaaacg ctcaatgccg ggccgtatta
    32581 tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg catggcgatg cagactcgcg
    32641 agcaacatat tcgtcgtgag aaagcgaact ccaacatttg tacttcccag gtactgctgg
    32701 caaacatcgc cagcctgtat gccgtttatc acggcccggt tggcctgaaa cgtatcgcta
    32761 accgcattca ccgtctgacc gatattctgg cggcgggcct gcaacaaaaa ggtctgaaac
    32821 tgcgccatgc gcactatttc gacaccttgt gtgtggaagt ggccgacaaa gcgggcgtac
    32881 tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga tatcctgaac gccgtgggga
    32941 tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca gcttttcagt gtgctgctgg
    33001 gcgataacca cgggctggag atcgacacgc tggacaaaga cgtggctcac gacagccgct
    33061 ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac ccatccggtg tttaatcgct
    33121 atcacagcga aaccgaaatg atgcgctata tgcactcgct ggagcgtaaa gatctggcgc
    33181 tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa actgaacgcc gccgccgaga
    33241 tgatcccaat cacttggacg gaatttgccg aactgcaccc gttttgcccg ccggaacagg
    33301 ctgaaggtta tcagcagatg attgcgcagc tggctgactg gctggtgaaa ctgaccggtt
    33361 acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg cgaatacgcg ggcctgctgg
    33421 cgattcgtca ttatcatgaa agccgcaacg aagggcatcg cgatatctgc ctgatcccgg
    33481 cttctgcgca cggaactaac cccgcttctg cacatatggc aggaatgcag gtggtggttg
    33541 tggcgtgtga taaaaacggc aacatcgatc tgactgatct gcgcgcgaaa gcggaacagg
    33601 cgggcgataa cctctcctgt atcatggtga cttatccttc tacccacggc gtgtatgaag
    33661 aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg cggtcaggtt taccttgatg
    33721 gcgcgaacat gaacgcccag gttggcatca cctcgccggg ctttattggc gcggacgttt
    33781 cgcacctcaa cctgcataaa actttctgca ttccgcacgg cggtggtggt ccgggtatgg
    33841 gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc gggtcatagc gtggtgcaaa
    33901 tcgaaggcat gttaacccgt cagggcgcag tttctgcggc accgttcggt agcgcctcta
    33961 tcctgccaat cagctggatg tacatccgca tgatgggcgc agaagggctg aaaaaagcaa
    34021 gccaggtggc aatcctcaac gccaactata ttgccagccg cctgcaggat gccttcccgg
    34081 tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg tattctcgat attcgcccgc
    34141 tgaaagaaga aaccggcatc agcgagctgg acattgccaa gcgcctgatc gactacggtt
    34201 tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct gatggttgaa ccgactgaat
    34261 ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat gctggctatc cgcgcagaaa
    34321 ttgaccaggt gaaagccggt gtctggccgc tggaagataa cccgctggtg aacgcgccgc
    34381 acattcagag cgaactggtc gccgagtggg cgcatccgta cagccgtgaa gttgcggtat
    34441 tcccggcagg cgtggcagac aaatactggc cgacggtgaa acgtctggat gatgtttacg
    34501 gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga ataccagtaa ttcactgatt
    34561 cgagtatttt ctaaaggcgc ttcggcgcct ttttagtcag atgacaaagt acaaaagtgc
    34621 tcagacagtc ccctcgcccc tttggggaga gggttagggt gaggggaaca ggccggcact
    34681 ggcgcgaata tttaccctca ccccgaccct ctccctgaaa gggcgagggg gaaaagcgtg
    34741 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt
    34801 tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg
    34861 ggcaactgca ttactgttgg cgcaagaagg gtatacggtg gcggttaatt atcagcaaaa
    34921 cctccacgcg gcgcaggaag tgatgaactt aataatgcaa gccggtggca aagcattcgt
    34981 gctccaggcg gatatcagcg acgaaaacca ggtcgttgcg atgtttacag caatcgatca
    35041 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
    35101 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg tgacgggata
    35161 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
    35221 cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga
    35281 ttacgcggca tcgaaagggg cgattgattc gttaaccacc ggactatcgc tggaagtcgc
    35341 cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg aaatgcacgc
    35401 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
    35461 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
    35521 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
    35581 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggc
    35641 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
    35701 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa
    35761 tgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacac catcgtaagt
    35821 cactgctttt ttcatctctt caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
    35881 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
    35941 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
    36001 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt caaaaccaga
    36061 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
    36121 aagataatcg caggtgcctt cgcgcagtac atcgagatcg ccgtcttcca ttttgatatt
    36181 aaatccgcga cgttcccact cgttcaatac ataggacggg taatagccgc atagctgcac
    36241 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
    36301 acatgagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
    36361 aatgcgacgc gcggctttca ctgccagggc gctggcgaca aactggtgat gcagcacctg
    36421 gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc acgccggagc agcagtaacc
    36481 aaacagcggc gcacgccagt tacgctggtt gtttatttcg ttgaaggtca tccagtattt
    36541 gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga aatcaacgaa
    36601 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
    36661 gagggttatc accggttcga tgttgtattt cagcagttca tcgaacagag agtcgtaaaa
    36721 cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac gcgtccaggc
    36781 gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
    36841 gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt
    36901 ggtgatttcg cgcggcacgc cgtgtgcgcc acaggtcaga acgtcacaaa tgctcggtcc
    36961 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc caccccataa
    37021 gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatcag tcaattctta
    37081 caggaaatag taccatgcag ccaaacgaca tcactttttt caacgtttcc aggatgacat
    37141 tctggctggg cgtaaaacca tcaccatccg cgacgagtct gaatcgcact tcaaaacggg
    37201 tgatgtgctt cgtgtcggac gttttgaaga tgacggttat ttttgcacga ttgaagtcac
    37261 cgcaacctca accgtaacgc tggatacact gacggaaaaa catgcagagc aggaaaatat
    37321 gaccctgcct gaactgaaaa aggtcattgc tgacatctat cccggtcaga cacaatttta
    37381 tatgattgaa tttaaatgtc tttaaattga actcacgcct gttagttgaa atttcgcatt
    37441 taatttcatg atttttagga gattagtctc aaatcagaat ttattttagc taacaggtgt
    37501 tcactggaac tattctcagt tacgctggag aggtatacat gtgcgtgtac gtttctctgg
    37561 agtaagttat ggttcagaag cccctcatta agcagggata ttcgctggca gaggaaattg
    37621 ccaacagcgt cagtcacggc attgggctgg tgtttggtat cgttgggctg gtgttgctac
    37681 tggttcaggc ggtggatctc aatgccagcg ccacagcgat aaccagttac agcctctatg
    37741 gcggcagtat gatcctgctg ttcctcgctt cgacgctcta tcacgccatt cctcatcaac
    37801 gggcaaaaat gtggctgaag aaatttgacc attgcgctat ttacctgttg attgccggaa
    37861 cctacacgcc gtttttgctg gtggggctgg attctccgtt agcgcgcggg ttgatgattg
    37921 ttatctggag cctagcattg ctggggattc tgttcaaact gaccatcgcg caccgattca
    37981 aaattttatc tctggtgact tatctggcga tgggctggct gtcgctggtg gtaatttatg
    38041 aaatggcagt taagctcgcg gcgggcagcg ttaccttact ggcggtaggc ggcgtggttt
    38101 attcgctcgg ggtgattttc tacgtctgca aacgcattcc atacaaccat gccatctggc
    38161 acggcttcgt gctcggcggt agtgtgtgcc actttctggc gatctatttg tatattgggc
    38221 aggcgtaatg ggtatgcctt tgtaggcctg ataagacgcg tcagcgtcgc atcaggcatc
    38281 ggagcgcaac cgccggatgc ggcgtgaacg ccttatccga tctacgggcc tcgtccctgt
    38341 tgtgataaag gcatcaaatc ccagacatga taagacgcgt cagcgtcgca tcaggcgata
    38401 aatgcctgat gcgatacaga gatttactct tcgagcgaat acggcagcgg ctcgatatgc
    38461 agtgtattcg catcgtcgcg tacgcggaag atgctgtccg gttccatatc gttattcatg
    38521 acgacctgta ccacgacctg accatcttcc agttttaccg cagccagcac cgtaccggta
    38581 cgacgccagt tctcgcccat tttcagctct aagtcttcac cagcttccgg cagtcggctg
    38641 gcactacctg ccagcaacca gagcgcgcgt ttgttggcac cacggaattt tgctcgcgcc
    38701 accatctctt gtccggtata acagcctttc ttaaagctga taccgcccag cgcctggaga
    38761 ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat caatcaccgg gaaaccagct
    38821 tcaatgttta atgccagcca ctgttggcta ttgttcagtt ccgcttcacc gcgcagttta
    38881 tcggtcagca tattagcagt agcttcatcg gttacgatca ggaaacgttc tgccgggtgt
    38941 tcaaaccata gcaaagtggt cgcgccttct ttgactacct gtttttcttt cgaaggcagt
    39001 tcgctaaaga gatttgccag cgcggcgcgc gcctgaaaac cggcaacacc aagcagcaca
    39061 cgctcgtcgt ctggcgcgat ggtcacttta gagaataccg catatttttt cagttcagtc
    39121 agctgcggtt cacgcacgct gcgccgttca atccatgcaa agccatcgcc gtcgcggaac
    39181 agacgtaaat tgctccacat tttacctttg gcgtcgcaat gggcggcgag caggtgctga
    39241 tcttctgcca tctggctgac atctgctgtc acctgaccct gcatatattt ttcgctgtcc
    39301 gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca tcagcgtcag cggtaaacgg
    39361 gcagaagccg taggctgacg tggaggaaaa ggtgtaaaag ccataataga gtcctgagtt
    39421 gcttaacggc gtattaatga ctaatggtaa aagagctatg aggcaatgca agtgctttca
    39481 ctacgcgatc tgtgccttct gcgaggcgac tgccaaaaaa atgaagccct tgaattgttg
    39541 tgaattaatt tcgcgatcgc gatggtgttt actgatattc ccgtaaaaga agtcgaaaaa
    39601 cacgttacaa tagcagggta ctggattttc gtgagacagg aagaagatgg acattaacaa
    39661 caaagcccga attcattggg catgccgccg tggtatgcgc gaactcgata tttcaatcat
    39721 gtcgtttttc gaacatgagt acgacagctt aagcgatgac gaaaaacgca tctttattcg
    39781 tctgctggaa tgtgacgatc cggacctgtt taactggctg atgaatcacg gtaaaccggc
    39841 cgatgcagaa ctggaaataa tggtccgact catccagaca cggaaccggg aacgtggtcc
    39901 tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg gctttccttg ctgattcatg
    39961 ggctggttgc cgctgttatt ttactcatgc cctggccgct cagttacacc ccgttatgga
    40021 tggtgttact ttcgctggtg gtatttgatt gcgttcgcag ccagcggtgt attaatgctc
    40081 gccaggggga aattcgcttg ttgatggacg ggcgtttgcg ttggcaaggg caggagtgga
    40141 gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat gctgcgttta cgttctgatg
    40201 gcggtaaacg gcaacattta tggctggcag ccgacagcat ggacgaagcc gaatggcggg
    40261 atttacggcg gattttgttg caacaagaga cgcaaagata aagatgacgg caaagcatat
    40321 gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca tttcgttgag gatttgctcg
    40381 caccagctct gaatacgctc gtcgctaagg tcatactggt tagtttcatc cagcgccaga
    40441 cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc tggtaaattc atatccttcc
    40501 gttggccagt agccgacgaa cttcacgcct ttggtcgaga gtttgtcatg cagcataccg
    40561 agcgcatcga ggaaccactc gccgtatccc agttgatcgc caagcccata cagcgcaata
    40621 attttacctt caaggttcag gtcgtcgagc tgatcccaga cggcttccca gtcttcctgg
    40681 atttcaccaa aatcccaggt cgggataccc agaatgagca catcgtactg ctccattaat
    40741 ttcggggagt cgtccttgag gttatgtaag gtcaccagtt ctgggccgat aatatcgcgg
    40801 attttttctg ccgccatttc ggtgtaacag gtgctggaac cgtaaaaaag acccatattc
    40861 ataacgtaag cttctcgatt ctgctgtggc ttttgcagct agtgtatcag aatcgcttta
    40921 gtcacggcac aataaggcat aatggatcaa atgagtgacc ataaggggcg caagtgaaac
    40981 aggagctggc acgcatcgag cagtttcttg atgctctgtg gctggaaaaa aatctggctg
    41041 aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat ggtggagtgg ttgcatcacc
    41101 gcgggttgac gctggcgacg gcgcaaagtg acgatttgca ggcattactg gcagaacggc
    41161 tggagggcgg gtataaagcc accagctcag cgcgtttgct gagtgcagtg cgccgattgt
    41221 tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc cagtgcgcat ctcgcttcac
    41281 cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc gcaggtcgaa cgtttattac
    41341 aggcgccatt aattgatcag ccactggagc tacgcgataa agccatgctt gaagtgttgt
    41401 atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac aatgagtgat atcagcctgc
    41461 gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga gcgcctggtg ccgttagggg
    41521 aagaggcggt ttactggctg gaaacctatc tggaacatgg gcgtccgtgg ctgctgaatg
    41581 gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca gcagatgacg cgacagacct
    41641 tctggcaccg cattaaacat tatgccgtgc tggcgggtat cgacagcgaa aagctgtcac
    41701 cgcatgtgtt gcgtcacgct tttgccactc atttattaaa tcatggtgcg gatttacgcg
    41761 tggtgcagat gctactgggc cacagcgatc tttccaccac gcaaatttat acgcatgtcg
    41821 ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc gcgggcgtga tgtctgaaaa
    41881 gaacgggaag atttatgaag aaaggtttta tgttgtttac tttgttagcg gcgttttcag
    41941 gctttgctca ggctgatgac gcggcaattc aacaaacgtt agccaaaatg ggcatcaaaa
    42001 gcagcgatat tcagcccgcg cccgtagctg gcatgaagac agttctgact aacagcggtg
    42061 tgttgtacat caccgatgat ggtaaacata tcattcaggg gccaatgtat gacgttagtg
    42121 gcacggctcc ggtcaatgtc accaataaga tgctgttaaa gcagttgaat gcgcttgaaa
    42181 aagagatgat cgtttataaa gcgccgcagg aaaaacacgt catcaccgtg tttactgata
    42241 ttacctgtgg ttactgccac aaactgcatg agcaaatggc agactataac gcgctgggga
    42301 tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga cagcgatgca gagaaagaaa
    42361 tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt tgatgatgtg atggcaggta
    42421 aaagcgtcgc accagccagc tgcgacgtgg atattgccga ccattacgca cttggcgtcc
    42481 agcttggcgt tagcggtact ccggcagttg tgctgagcaa tggcacactt gttccgggtt
    42541 accagccgcc gaaagacatg aaagaattcc tcgacgaaca ccaaaaaatg accagcggta
    42601 aataattcgc gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga
    42661 cttgcccgct gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag
    42721 tgcgcaagaa ctggaacgca gtgttaaagg tatgttgccc tggcagcaac taagcggtgt
    42781 cgaaaaggcc gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt
    42841 cggtgatttc gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc
    42901 gcttggttgc agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt
    42961 aagcccggaa gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga
    43021 taacggtatt tcctcccatg cgggagttga gcacgctcgc tcgttgggca tcccggttat
    43081 tgttaccgat caccatttgc cgggcgacac attacccgca gcggaagcga tcattaaccc
    43141 taacttgcgc gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta
    43201 tctgatgctg gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgcggcat
    43261 cgcaattcct aacctggcag aactgctgga tctggtagca ctggggacag tagcggacgt
    43321 cgtgccgctg gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgtgc
    43381 cggaaagtgc cgtccaggga ttaaagcgct gctggaagtg gcaaatcgcg atgcacaaaa
    43441 actagccgcc agcgatttag gttttgcgct gggaccgcgt ctcaatgctg ccgggcgact
    43501 ggatgatatg tccgttggtg tggcgctgtt gctgtgcgac aacatcggcg aagcgcgcgt
    43561 gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca
    43621 ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg
    43681 gctggcaatg tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgtat
    43741 caaagagcgt tttcaccgtc cggttatcgc ctttgcgcca gcaggtgacg gtacgctgaa
    43801 aggttccggt cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac
    43861 actctacccc ggcatgatgc tcaagtttgg cggtcatgcg atggcggcgg gtttgtcgct
    43921 ggaagaggat aaattcgaac tctttcaaca acggtttggc gaactggtta ctgagtggct
    43981 ggccccttcg ctattgcaag gcgaagtggt gtcagacggt ccgttaagcc cggccgaaat
    44041 gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga
    44101 gccgctgttt gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt
    44161 gaaggtgatg gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt
    44221 cgataccgct ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga
    44281 tatcaacgag tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat
    44341 ttagcgtcat cttctctata aaaaagagcg tggattgggt acaatcccgc tcttatcacc
    44401 gcattttgac tagctcaata aaagaaatca gaccatgttt gaaattaatc cggtaaataa
    44461 tcgcattcag gacctcacgg aacgctccga cgttcttagg gggtatcttt gactacgacg
    44521 ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga acagccggat gtctggaacg
    44581 aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc cctcgaagcc gttgtcgaca
    44641 ccctcgacca aatgaaacag gggctggaag atgtttctgg tctgctggaa ctggctgtag
    44701 aagctgacga cgaagaaacc tttaacgaag ccgttgctga actcgacgcc ctggaagaaa
    44761 aactggcgca gcttgagttc cgccgtatgt tctctggcga atatgacagc gccgactgtt
    44821 acctcgatat tcaggcgggg tctggcggta cggaagcaca ggactgggcg agcatgcttg
    44881 agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa aactgaaatc atcgaagagt
    44941 cggaaggtga agtggcgggt attaaatccg tgacgatcaa aatctccggc gattacgctt
    45001 acggctggct gcgtacagaa actggcgttc accgcctggt gcgtaaaagc ccgtttgact
    45061 ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt ttatccggaa gttgatgatg
    45121 atattgatat cgaaatcaac ccggcggatc tgcgcattga cgtttatcgc gcgtccggcg
    45181 cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg tattacccac atcccgaccg
    45241 ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa gaacaaagat caggccatga
    45301 agcagatgaa agcgaagctt tatgaactgg agatgcagaa gaaaaatgcc gagaaacagg
    45361 cgatggaaga taacaaatct gacatcggct ggggcagcca gattcgttct tatgtccttg
    45421 atgactcccg cattaaagat ctgcgcaccg gggtagaaac ccgcaacacg caggccgtgc
    45481 tggacggcag cctggatcaa tttatcgaag caagtttgaa agcagggtta tgaggaacca
    45541 acatgtctga acaacacgca cagggcgctg acgcggtagt cgatcttaac aatgaactga
    45601 aaacgcgtcg tgagaagctg gcgaacctgc gtgagcaggg aattgccttc ccgaacgatt
    45661 tccgtcgcga tcatacctct gaccaattgc acgcagaatt cgacggtaaa gagaacgaag
    45721 aactggaagc gctgaacatc gaagtcgccg ttgctggccg catgatgacc cgccgtatta
    45781 tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg ccgcattcag ctgtacgttg
    45841 cccgtgacga tctgccggaa ggcgtttaca acgagcagtt caaaaaatgg gacctcggcg
    45901 acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac cggcgaactg tctatccact
    45961 gtaccgagct gcgtctgctg accaaagcac tgcgtccgct gccggataaa ttccacggct
    46021 tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga tctcatctct aacgatgaat
    46081 cccgtaacac ctttaaagta cgctcgcaga tcctctctgg tattcgccag ttcatggtga
    46141 accgcggctt tatggaagtt gaaacgccga tgatgcaggt gatcccaggc ggtgccgctg
    46201 cgcgtccgtt tatcacccac cataacgcgc tggatctcga catgtacctg cgtatcgcgc
    46261 cggaactgta cctcaagcgt ctggtggtcg gtggcttcga gcgtgtattc gaaatcaacc
    46321 gtaacttccg taacgaaggt atttccgtac gtcataaccc agagttcacc atgatggaac
    46381 tctacatggc ttacgcagat tacaaagatc tgatcgagct gaccgaatcg ctgttccgta
    46441 ctctggcaca ggatattctc ggtaagacgg aagtgaccta cggcgacgtg acgctggact
    46501 tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat caagaaatat cgcccggaaa
    46561 ccgacatggc ggatctggat aacttcgact ctgcgaaagc gattgctgaa tctatcggta
    46621 tccacgttga gaagagctgg ggtctgggcc gtatcgttac cgagatcttc gaagaagtgg
    46681 cagaagcaca tctgattcag ccgaccttca ttactgaata tccggcagaa gtttctccgc
    46741 tggcgcgtcg taacgacatt aacccggaaa tcacagaccg ctttgagttc ttcatcggtg
    46801 gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc ggaagatcag gcgcaacgct
    46861 tcctggatca ggttgccgcg aaagacgcag gtgacgacga agcgatgttc tacgatgaag
    46921 attacgttac cgcactggaa catggcttac cgccgacagc aggtctgggg attggcatcg
    46981 accgtatggt aatgctgttc accaacagcc ataccatccg cgacgttatt ctgttcccgg
    47041 cgatgcgtcc ggtgaaataa gcattacgtt atgctcacaa ccccggcaaa tgtcggggtt
    47101 ttttatttaa gctgggtaaa tgcagataat cgtattctgg cttcgcgatt tgtcgcctgc
    47161 atcaccatcc acggactgaa cgcccacggc gtggcatcaa taccgcgtaa tacatcagct
    47221 aaatcacacc attgataatc catcacttca tcatcgttga tctgtaacgc actggtggtg
    47281 cgtgcggcaa ataccggaca cacttcattt tccacaatgc ctctcggatc ggtggcgcgg
    47341 tagcgaaagt caggatagat agattcagga ggcgtaattt ccacgccaag ctcataacgg
    47401 caacggcgga tcaccgcgtc ttcgctgctt tctcccagtt gtgggtgccc acaaaccgag
    47461 ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc ggcgggtaac taataattgc
    47521 cccttggcat taaacagcca actggagaac gcgagatgta agagggtgtc tgccgtgtgt
    47581 gcggcatact tttccagcgt acccgtggga actccctgtg cattcaataa aatgacgtgt
    47641 tccgtttgca taatttctca catgtaattc tgatcactca tcattataga taattgaaga
    47701 gtgaaatgat taaccaagag agcgtttatc gacaaccgcc cgttttgagc ggatgccgac
    47761 aggtaaaaac catttaaaaa agtagcatca tatatttgaa gcgactttca atggccattg
    47821 caacacagtc agaaatgaca ggaatagaga gagtaaaaag caacaacaga ctgaatatag
    47881 cgcgcattaa aaaattcata ttattctcca tgctcatttt tcttcagctt gcgcatcaga
    47941 agccacatac gtactgtcct gacttctaaa tctttatcag agacggattc cttcaccagg
    48001 tcagcatgat gctgatagcc attaaagaag acgttcagca caacggcgct aagggttgcg
    48061 agcataatgc cgctatgtag cagcggttgc agtacggccg gtaacttaga aaagaaatcg
    48121 tgagagagcg tcggagtcat gccaacgccg agactgatcg ccacaatata gaggttgtaa
    48181 cggttggtgg tgtagttaca gcgcgacaga attcgaatcc ctgtcgccag taccatgccg
    48241 aacatcacta gaccagcgcc gcccagcaca aattgcggaa tggaggctac cagcaccgcc
    48301 atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca cacccagcgg
    48361 ctatgaacgc gcatcacgct aaccaggcca acgttttgag aaaatgacgt gtgggggaag
    48421 ctgttaaacg tgccgcctat cattgtccct acgccatcga cacgcagccc gcaaataata
    48481 tcgtgtgaag agagtttacg accgactatt tcacccagtg ccaggaacat ccccatcgac
    48541 tcgataaaca cgatgattaa cacggcagtc atggtcagaa tggaaacggg atcgaaaatc
    48601 ggcataccaa acgacatcgg ggtgacaatc gcaaaccacg aggcatcatg tagcccggat
    48661 aaattgactt cattcatcat ccacgaaagt aaaaagccaa atacaatccc cagtaatacg
    48721 gcgacgttgg acataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagacg
    48781 gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt acctccagcg
    48841 gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta
    48901 accagtggcg ggaataaagg catcaagcga ccaataagtg gcgctaataa tgtggtgata
    48961 aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca
    49021 atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc
    49081 cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga
    49141 tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata
    49201 agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc
    49261 agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat
    49321 gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagaataaa
    49381 aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg
    49441 cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca
    49501 cgaaaatgac aagagaaact gacaatggaa ttatctgtaa ttttctcata ttgatatttc
    49561 attaatcatt ctggcatgtc gcactctcgc atttaatcgt ttttatctgg atagcgctct
    49621 tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact
    49681 cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc
    49741 gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat acgtttatcg
    49801 ctgctgaagc tgcggaatgt ataggctgtc a
//
LOCUS       DBBBSB010000015        49538 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.015, whole genome shotgun sequence.
ACCESSION   DBBBSB010000015 DBBBSB010000000
VERSION     DBBBSB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49538
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.015"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="ACIUS0_000846"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="ACIUS0_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGB0218716.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="ACIUS0_000847"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="ACIUS0_000847"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGB0218717.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="ACIUS0_000848"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="ACIUS0_000848"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGB0218718.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="ACIUS0_000849"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="ACIUS0_000849"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGB0218719.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="ACIUS0_000850"
     CDS             5724..6665
                     /locus_tag="ACIUS0_000850"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGB0218720.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="ACIUS0_000851"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="ACIUS0_000851"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGB0218721.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="ACIUS0_000852"
     CDS             7685..8935
                     /locus_tag="ACIUS0_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGB0218722.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /locus_tag="ACIUS0_000853"
                     /pseudo
     CDS             complement(9006..9700)
                     /locus_tag="ACIUS0_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="ACIUS0_000854"
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="ACIUS0_000854"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGB0218723.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10872..12122
                     /locus_tag="ACIUS0_000855"
     CDS             10872..12122
                     /locus_tag="ACIUS0_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGB0218724.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="ACIUS0_000856"
     CDS             complement(12178..13266)
                     /locus_tag="ACIUS0_000856"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGB0218725.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="ACIUS0_000857"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="ACIUS0_000857"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGB0218726.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="ACIUS0_000858"
     CDS             complement(14200..15249)
                     /locus_tag="ACIUS0_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGB0218727.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /gene="yieE"
                     /locus_tag="ACIUS0_000859"
     CDS             15348..16229
                     /gene="yieE"
                     /locus_tag="ACIUS0_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGB0218728.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="ACIUS0_000860"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="ACIUS0_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGB0218729.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="ACIUS0_000861"
                     /pseudo
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="ACIUS0_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="ACIUS0_000862"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="ACIUS0_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="ACIUS0_000863"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="ACIUS0_000863"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGB0218730.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /gene="yeiB"
                     /locus_tag="ACIUS0_000864"
     CDS             21997..23073
                     /gene="yeiB"
                     /locus_tag="ACIUS0_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGB0218731.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="ACIUS0_000865"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="ACIUS0_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGB0218732.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="ACIUS0_000866"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="ACIUS0_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGB0218733.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="ACIUS0_000867"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="ACIUS0_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGB0218734.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="ACIUS0_000868"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="ACIUS0_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGB0218735.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="ACIUS0_000869"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="ACIUS0_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="ACIUS0_000870"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="ACIUS0_000870"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGB0218736.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="ACIUS0_000871"
     CDS             complement(30815..31054)
                     /locus_tag="ACIUS0_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGB0218737.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="ACIUS0_000872"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="ACIUS0_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGB0218738.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="ACIUS0_000873"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="ACIUS0_000873"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGB0218739.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="ACIUS0_000874"
     CDS             complement(32940..33635)
                     /locus_tag="ACIUS0_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGB0218740.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="ACIUS0_000875"
     CDS             complement(33632..34030)
                     /locus_tag="ACIUS0_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGB0218741.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="ACIUS0_000876"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="ACIUS0_000876"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGB0218742.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="ACIUS0_000877"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="ACIUS0_000877"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGB0218743.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="ACIUS0_000878"
                     /pseudo
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="ACIUS0_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37422..38144
                     /locus_tag="ACIUS0_000879"
                     /pseudo
     CDS             37422..38144
                     /locus_tag="ACIUS0_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="ACIUS0_000880"
     CDS             complement(38261..38839)
                     /locus_tag="ACIUS0_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGB0218744.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="ACIUS0_000881"
     CDS             39009..39596
                     /locus_tag="ACIUS0_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGB0218745.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="ACIUS0_000882"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="ACIUS0_000882"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGB0218746.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="ACIUS0_000883"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="ACIUS0_000883"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGB0218747.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="ACIUS0_000884"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="ACIUS0_000884"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGB0218748.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46117
                     /gene="osmF"
                     /locus_tag="ACIUS0_000885"
     CDS             45200..46117
                     /gene="osmF"
                     /locus_tag="ACIUS0_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGB0218749.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46124..47281
                     /gene="yehY"
                     /locus_tag="ACIUS0_000886"
     CDS             46124..47281
                     /gene="yehY"
                     /locus_tag="ACIUS0_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGB0218750.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47274..48200
                     /gene="yehX"
                     /locus_tag="ACIUS0_000887"
     CDS             47274..48200
                     /gene="yehX"
                     /locus_tag="ACIUS0_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGB0218751.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48205..48936
                     /gene="yehW"
                     /locus_tag="ACIUS0_000888"
     CDS             48205..48936
                     /gene="yehW"
                     /locus_tag="ACIUS0_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGB0218752.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48917..49024)
                     /locus_tag="ACIUS0_000889"
     CDS             complement(48917..49024)
                     /locus_tag="ACIUS0_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGB0218753.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49084..49538)
                     /locus_tag="ACIUS0_000890"
                     /pseudo
     CDS             complement(49084..49538)
                     /locus_tag="ACIUS0_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11960 a        12205 c        13312 g        12061 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggccag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtggtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgtgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagaaccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca gaatgtctga
     6661 attaaaaatt tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccagcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ccaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 atttatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg caaatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtacgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagctggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggtgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aacatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacgcaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctagacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggg gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggt ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac gctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc ggatctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatc gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatca
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggacgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ttttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct ctggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcggct
    34921 gcgacgcagt gatgattggt cgcggggcgc tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagc cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcatttag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagatccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attacaccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ctgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat gccgacaagc
    44521 cgaacaaata tacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat cttgcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccga
    45421 tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta aagatgaaaa
    45481 cgatgcagcg tggaaaaatg ccgggcaggg ctacgagaaa gtcaaaaagc tcgatgcgga
    45541 gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga ctatcgccgt
    45601 gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gccgacctga gtcgttatct
    45661 gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac gcgccgatgc
    45721 gttacccacg tttgaaaaag cctatggctt taagctcggt caggatcagt tgctgtcact
    45781 ggctggtggc gacacggcgg tgacgattaa agccgctgcc cagcaaacct ctggcgttaa
    45841 tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc aaaccttaag
    45901 cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg aatcggtgtt
    45961 gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc tcgatgcaaa
    46021 aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca aaaaagtggc
    46081 tgccgactac ctgaaacaaa aagggtgggc gaagtaattt cccgtgactt atctacgtat
    46141 taatcctgtt ctggcgctgc tgttgttgct gacggcaatc gcagcggcgc tgccgtttat
    46201 cagttacgcg cctaatcgtt tagtctcggg tgaagggctt catctctggc aactgtggcc
    46261 gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct gttttgtccc
    46321 cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg tattgttggt
    46381 gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc tggcgcgtac
    46441 cagcctcggc agaggtttct ggctggctgc ggcgctggca ttgctggcct gtagcgatgc
    46501 catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc agattgccat
    46561 tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa tgaaagaata
    46621 cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt tgctgtttgg
    46681 tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct acttttccac
    46741 tgcgcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc cttcggtggc
    46801 gctctttggc ctgttgattg cgccgcttgc cgcgctggtt acggcctttc cgtggctggg
    46861 gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg tgctctatgc
    46921 cttgctgccg ctggtgcgag gcgtggtagt cggtttgaac cagatcccgc gcgatgtgct
    46981 ggagagcgcc agagcgatgg ggatgagcgg ggcgcagcgt ttcctgcatg ttcagttacc
    47041 gctggcgtta ccggtatttc tgcgcagcct gcgggtggtg atggtgcaaa ctgtaggtat
    47101 ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt tccaggggct
    47161 gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg ttctggcggt
    47221 gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac gtaatgattg
    47281 aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat ctcaatctca
    47341 attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc aaatccacca
    47401 ccctgaaaat gattaaccgc ctggtggagc atgacagcgg agtgatccgc tttgccggag
    47461 aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat gccattcaat
    47521 ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg ccgcaattac
    47581 aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg ctactggggc
    47641 tggagtcaaa tttgcgtgag cgttatccgc atcagctttc cggtggtcag cagcaacgtg
    47701 tgggagtggc gcgtgcactg gctgccgatc cgcaagtctt actgatggat gaaccttttg
    47761 gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc attcaccgtt
    47821 tgctggggcg cactattgtg ctggtcactc atgatattga tgaggcgcta cggctggcag
    47881 aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg ctgacgatgc
    47941 tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa ctgggggtgc
    48001 acctgctttc gttacgtagc gtggcggatt acgtgcgtcg cgaagaacgg gcagaaggtg
    48061 aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc gcgcggggat
    48121 gcgaggtgct gccggtggtg aacacgcagg gccagccttg cggcacgctg cattttcagg
    48181 atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt ctggctcatt
    48241 gctctttttg tggcgctgat tttctggctg ccttacagcc agccgctgtt tgctgccttg
    48301 ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct ggcactggct
    48361 catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggcac aggtgccgga
    48421 attgctgtta ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac tattgccgcc
    48481 gttggacaga cttttccgcc tgtcgcagtg ctggcgattg ccgttccggt gatcggcttt
    48541 ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccat cctgcaggtg
    48601 acacttgccg ggctgggagc gattgatccc agcgtgacag aagttgcgaa aggtatggga
    48661 atgagtcgag gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc ggtgattctg
    48721 gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc ctcaacggta
    48781 ggggccagta cgctgggtac gcccatcatc atcgggctta gcggatttaa taccgcgtat
    48841 gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg cctgtttgaa
    48901 aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg agcatgacgc
    48961 caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt ttagctatgc
    49021 gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt tgttaacgaa
    49081 tcattaaata ccgagtggga aaatatcatg gccttgttct tgccaactgg tgagttgctg
    49141 ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc cttcgaatag
    49201 ttcagggcgt agttgattga gcgagtgggc gagcgaggac atcaatgcgc catccttgtt
    49261 gactggcaat ccagccctcc agccacagac gggtggtatc ctgaatattc cagccaacca
    49321 ccagcgcatc tttaccctgt tttttacgtg ccgaagccag acaaatggcg atgtagttga
    49381 tcagtacgcc gtcgaggatc gccagcagcg cctggagagt cggttgttgg cactgaagcc
    49441 gtcggcgcag aggaataaac agatgtgtgg tgagtgtctg ggcggggtaa tcctgaccgc
    49501 gctctttgat ccacgttcgc aggctatgta gattgccg
//
LOCUS       DBBBSB010000016        48321 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.016, whole genome shotgun sequence.
ACCESSION   DBBBSB010000016 DBBBSB010000000
VERSION     DBBBSB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48321
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.016"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            187..480
                     /gene="yicS"
                     /locus_tag="ACIUS0_000891"
     CDS             187..480
                     /gene="yicS"
                     /locus_tag="ACIUS0_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGB0218754.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACIUS0_000892"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="ACIUS0_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGB0218755.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="ACIUS0_000893"
     CDS             complement(1922..2374)
                     /locus_tag="ACIUS0_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGB0218756.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACIUS0_000894"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="ACIUS0_000894"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGB0218757.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="ACIUS0_000895"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="ACIUS0_000895"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGB0218758.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACIUS0_000896"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="ACIUS0_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGB0218759.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACIUS0_000897"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="ACIUS0_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGB0218760.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACIUS0_000898"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="ACIUS0_000898"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGB0218761.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACIUS0_000899"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="ACIUS0_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGB0218762.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACIUS0_000900"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="ACIUS0_000900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGB0218763.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACIUS0_000901"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="ACIUS0_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGB0218764.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACIUS0_000902"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="ACIUS0_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGB0218765.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="ACIUS0_000903"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="ACIUS0_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGB0218766.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACIUS0_000904"
     CDS             complement(13737..13811)
                     /gene="ysdE"
                     /locus_tag="ACIUS0_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGB0218767.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="ACIUS0_000905"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="ACIUS0_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGB0218768.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="ACIUS0_000906"
     CDS             complement(15085..15582)
                     /locus_tag="ACIUS0_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGB0218769.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="ACIUS0_000907"
     CDS             complement(15579..15941)
                     /locus_tag="ACIUS0_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGB0218770.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="ACIUS0_000908"
     CDS             complement(15931..16278)
                     /locus_tag="ACIUS0_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGB0218771.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /gene="yidI"
                     /locus_tag="ACIUS0_000909"
     CDS             16387..16800
                     /gene="yidI"
                     /locus_tag="ACIUS0_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGB0218772.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="ACIUS0_000910"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="ACIUS0_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="ACIUS0_000911"
     CDS             complement(18324..20039)
                     /locus_tag="ACIUS0_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGB0218773.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="ACIUS0_000912"
     CDS             20206..21099
                     /locus_tag="ACIUS0_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGB0218774.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="ACIUS0_000913"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="ACIUS0_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="ACIUS0_000914"
     CDS             complement(21910..23526)
                     /locus_tag="ACIUS0_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGB0218775.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="ACIUS0_000915"
     CDS             23821..24495
                     /locus_tag="ACIUS0_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGB0218776.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="ACIUS0_000916"
     CDS             complement(24534..26195)
                     /locus_tag="ACIUS0_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGB0218777.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACIUS0_000917"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="ACIUS0_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGB0218778.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACIUS0_000918"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="ACIUS0_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGB0218779.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="ACIUS0_000919"
     CDS             27649..27981
                     /locus_tag="ACIUS0_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGB0218780.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="ACIUS0_000920"
     CDS             complement(27983..29215)
                     /locus_tag="ACIUS0_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGB0218781.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="ACIUS0_000921"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="ACIUS0_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGB0218782.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="ACIUS0_000922"
     CDS             complement(30377..31714)
                     /locus_tag="ACIUS0_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0218783.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACIUS0_000923"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="ACIUS0_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACIUS0_000924"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="ACIUS0_000924"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGB0218784.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACIUS0_000925"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="ACIUS0_000925"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGB0218785.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACIUS0_000926"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="ACIUS0_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGB0218786.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /gene="yidX"
                     /locus_tag="ACIUS0_000927"
     CDS             35377..36033
                     /gene="yidX"
                     /locus_tag="ACIUS0_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGB0218787.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACIUS0_000928"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="ACIUS0_000928"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGB0218788.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="ACIUS0_000929"
     CDS             complement(37005..37403)
                     /locus_tag="ACIUS0_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGB0218789.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACIUS0_000930"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="ACIUS0_000930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGB0218790.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACIUS0_000931"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="ACIUS0_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGB0218791.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACIUS0_000932"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="ACIUS0_000932"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGB0218792.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACIUS0_000933"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="ACIUS0_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGB0218793.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="ACIUS0_000934"
     CDS             43964..44044
                     /locus_tag="ACIUS0_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218794.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="ACIUS0_000935"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="ACIUS0_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGB0218795.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="ACIUS0_000936"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="ACIUS0_000936"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGB0218796.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="ACIUS0_000937"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="ACIUS0_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGB0218797.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="ACIUS0_000938"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="ACIUS0_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGB0218798.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="ACIUS0_000939"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="ACIUS0_000939"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGB0218799.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11980 a        13065 c        12219 g        11057 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca gaatagacaa a
//
LOCUS       DBBBSB010000017        48062 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.017, whole genome shotgun sequence.
ACCESSION   DBBBSB010000017 DBBBSB010000000
VERSION     DBBBSB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48062
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.017"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACIUS0_000940"
     CDS             complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACIUS0_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGB0218800.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACIUS0_000941"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACIUS0_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGB0218801.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="ACIUS0_000942"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="ACIUS0_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGB0218802.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="ACIUS0_000943"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="ACIUS0_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGB0218803.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACIUS0_000944"
     CDS             complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACIUS0_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGB0218804.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACIUS0_000945"
     CDS             complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACIUS0_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGB0218805.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9762..10139
                     /locus_tag="ACIUS0_000946"
     CDS             9762..10139
                     /locus_tag="ACIUS0_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGB0218806.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10292..10843
                     /gene="apt"
                     /locus_tag="ACIUS0_000947"
     CDS             10292..10843
                     /gene="apt"
                     /locus_tag="ACIUS0_000947"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGB0218807.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10972..12897
                     /gene="dnaX"
                     /locus_tag="ACIUS0_000948"
     CDS             10972..12897
                     /gene="dnaX"
                     /locus_tag="ACIUS0_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGB0218808.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12230..12294
                     /gene="dnaX"
                     /locus_tag="ACIUS0_000948"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12950..13279
                     /locus_tag="ACIUS0_000949"
     CDS             12950..13279
                     /locus_tag="ACIUS0_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGB0218809.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13279..13884
                     /gene="recR"
                     /locus_tag="ACIUS0_000950"
     CDS             13279..13884
                     /gene="recR"
                     /locus_tag="ACIUS0_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGB0218810.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13994..15868
                     /gene="htpG"
                     /locus_tag="ACIUS0_000951"
     CDS             13994..15868
                     /gene="htpG"
                     /locus_tag="ACIUS0_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGB0218811.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16049..16693
                     /gene="adk"
                     /locus_tag="ACIUS0_000952"
     CDS             16049..16693
                     /gene="adk"
                     /locus_tag="ACIUS0_000952"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGB0218812.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16825..17787
                     /gene="hemH"
                     /locus_tag="ACIUS0_000953"
     CDS             16825..17787
                     /gene="hemH"
                     /locus_tag="ACIUS0_000953"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGB0218813.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACIUS0_000954"
                     /pseudo
     CDS             complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACIUS0_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18895..20199
                     /gene="gsk"
                     /locus_tag="ACIUS0_000955"
     CDS             18895..20199
                     /gene="gsk"
                     /locus_tag="ACIUS0_000955"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGB0218814.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACIUS0_000956"
     CDS             complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACIUS0_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGB0218815.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22246..23466)
                     /gene="fsr"
                     /locus_tag="ACIUS0_000957"
     CDS             complement(22246..23466)
                     /gene="fsr"
                     /locus_tag="ACIUS0_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGB0218816.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23684..25336
                     /gene="ushA"
                     /locus_tag="ACIUS0_000958"
     CDS             23684..25336
                     /gene="ushA"
                     /locus_tag="ACIUS0_000958"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGB0218817.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25373..25852)
                     /gene="ybaK"
                     /locus_tag="ACIUS0_000959"
     CDS             complement(25373..25852)
                     /gene="ybaK"
                     /locus_tag="ACIUS0_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGB0218818.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26056..26850)
                     /locus_tag="ACIUS0_000960"
     CDS             complement(26056..26850)
                     /locus_tag="ACIUS0_000960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGB0218819.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26988..27329
                     /locus_tag="ACIUS0_000961"
     CDS             26988..27329
                     /locus_tag="ACIUS0_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGB0218820.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27544..30048)
                     /gene="copA"
                     /locus_tag="ACIUS0_000962"
     CDS             complement(27544..30048)
                     /gene="copA"
                     /locus_tag="ACIUS0_000962"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGB0218821.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30310..31242
                     /gene="glsA"
                     /locus_tag="ACIUS0_000963"
     CDS             30310..31242
                     /gene="glsA"
                     /locus_tag="ACIUS0_000963"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGB0218822.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31245..32537
                     /locus_tag="ACIUS0_000964"
     CDS             31245..32537
                     /locus_tag="ACIUS0_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGB0218823.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32568..32665
                     /locus_tag="ACIUS0_000965"
                     /pseudo
     CDS             32568..32665
                     /locus_tag="ACIUS0_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32662..33069
                     /gene="cueR"
                     /locus_tag="ACIUS0_000966"
     CDS             32662..33069
                     /gene="cueR"
                     /locus_tag="ACIUS0_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGB0218824.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33070..33528)
                     /locus_tag="ACIUS0_000967"
     CDS             complement(33070..33528)
                     /locus_tag="ACIUS0_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGB0218825.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33525..34442)
                     /locus_tag="ACIUS0_000968"
     CDS             complement(33525..34442)
                     /locus_tag="ACIUS0_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGB0218826.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34588..35265
                     /gene="fetA"
                     /locus_tag="ACIUS0_000969"
     CDS             34588..35265
                     /gene="fetA"
                     /locus_tag="ACIUS0_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGB0218827.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35252..36031
                     /gene="fetB"
                     /locus_tag="ACIUS0_000970"
     CDS             35252..36031
                     /gene="fetB"
                     /locus_tag="ACIUS0_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGB0218828.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36094..36948)
                     /gene="cnoX"
                     /locus_tag="ACIUS0_000971"
     CDS             complement(36094..36948)
                     /gene="cnoX"
                     /locus_tag="ACIUS0_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGB0218829.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37009..37818)
                     /gene="ybbO"
                     /locus_tag="ACIUS0_000972"
     CDS             complement(37009..37818)
                     /gene="ybbO"
                     /locus_tag="ACIUS0_000972"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGB0218830.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37808..38430)
                     /gene="tesA"
                     /locus_tag="ACIUS0_000973"
                     /pseudo
     CDS             complement(37808..38430)
                     /gene="tesA"
                     /locus_tag="ACIUS0_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38401..39087
                     /gene="ybbA"
                     /locus_tag="ACIUS0_000974"
     CDS             38401..39087
                     /gene="ybbA"
                     /locus_tag="ACIUS0_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGB0218831.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39084..41499
                     /gene="ybbP"
                     /locus_tag="ACIUS0_000975"
                     /pseudo
     CDS             39084..41499
                     /gene="ybbP"
                     /locus_tag="ACIUS0_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41717..41872)
                     /gene="dinQ"
                     /locus_tag="ACIUS0_000976"
     CDS             complement(41717..41872)
                     /gene="dinQ"
                     /locus_tag="ACIUS0_000976"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGB0218832.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42212..43306)
                     /gene="mnmH"
                     /locus_tag="ACIUS0_000977"
     CDS             complement(42212..43306)
                     /gene="mnmH"
                     /locus_tag="ACIUS0_000977"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGB0218833.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43375..44301)
                     /gene="allS"
                     /locus_tag="ACIUS0_000978"
     CDS             complement(43375..44301)
                     /gene="allS"
                     /locus_tag="ACIUS0_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGB0218834.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44531..45013
                     /gene="allA"
                     /locus_tag="ACIUS0_000979"
     CDS             44531..45013
                     /gene="allA"
                     /locus_tag="ACIUS0_000979"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGB0218835.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45091..45906
                     /gene="allR"
                     /locus_tag="ACIUS0_000980"
     CDS             45091..45906
                     /gene="allR"
                     /locus_tag="ACIUS0_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGB0218836.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45996..47777
                     /gene="gcl"
                     /locus_tag="ACIUS0_000981"
     CDS             45996..47777
                     /gene="gcl"
                     /locus_tag="ACIUS0_000981"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGB0218837.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47790..>48062
                     /locus_tag="ACIUS0_000982"
     CDS             47790..>48062
                     /locus_tag="ACIUS0_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGB0218838.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11432 a        12507 c        12792 g        11331 t
ORIGIN      
        1 ttttatgaat tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg
      121 attacatgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc
      601 agctctcgta cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt
     2041 tttctaccac aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagctc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 catcacccac gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca
     3541 tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg
     3841 ctggactggg tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcacagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacctg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct
     7021 tccgctgtgg gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag catcacgctc tggcattaca acggcactga agctggcgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg
     8041 aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg
     8761 acaccagcgg ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac
     8821 aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc
     8881 aagaattcaa tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt
     8941 ttgcgtttgc ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg
     9001 cctcatcatc gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca
     9061 gcacggtact ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt
     9121 tgacatcgtc aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc
     9181 gctagcctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag
     9241 gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg
     9301 gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt
     9361 ttacgctgcc agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg
     9421 gaggcttcac gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact
     9481 tgcggcagct gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg
     9541 tcgagacaag actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca
     9601 cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct
     9661 tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg
     9721 ttatgctaaa gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa
     9781 tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac
     9841 cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc
     9901 acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc
     9961 atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca
    10021 tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct
    10081 gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa
    10141 gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca
    10201 caggcgcagg cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt
    10261 gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta
    10321 tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga
    10381 tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga
    10441 gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt
    10501 tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa
    10561 actgccgcgt gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga
    10621 aatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc
    10681 aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc
    10741 tgacgctgcg ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca
    10801 gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg
    10861 ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt
    10921 gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat
    10981 caggttttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat
    11041 gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt
    11101 tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac
    11161 tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag
    11221 caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat
    11281 acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat
    11341 ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt
    11401 gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg
    11461 gtgacgattt tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt
    11521 cgccatcagc ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg
    11581 caattgttgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag
    11641 gcgattgcca gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg
    11701 cttgacgacg atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc
    11761 gtaatggcac tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg
    11821 gaaatgctcg gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc
    11881 aacgacatgg ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg
    11941 gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg
    12001 gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg
    12061 ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta
    12121 atgacgccaa ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc
    12181 ccggaaacca ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca
    12241 accaaagcaa aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct
    12301 gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg
    12361 ctggaaaaag cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg
    12421 cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa
    12481 acgccggaac tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca
    12541 caggtgagcc aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa
    12601 gaggagagcg acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac
    12661 cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa
    12721 ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg
    12781 atatacgaag aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag
    12841 accctgcgtc ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg
    12901 taagcacagc ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa
    12961 aggcggtctg ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat
    13021 gcaggaagag atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt
    13081 gaccatcaac ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga
    13141 cgacaaagag atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat
    13201 tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg
    13261 ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg
    13321 cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag
    13381 cgcgatcgta gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc
    13441 ggccactgcg ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat
    13501 ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc
    13561 attgagcaga cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg
    13621 ctggacggca tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag
    13681 gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct
    13741 aactacattg ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc
    13801 gtaccggttg gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc
    13861 gggcgtcata agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa
    13921 ttattctccc ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt
    13981 gaggtagacc tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct
    14041 tctgcacctg atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat
    14101 ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct
    14161 gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct
    14221 gaccatctcc gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac
    14281 tatcgctaaa tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga
    14341 cagccagctg atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa
    14401 agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc
    14461 ggctggcgaa ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat
    14521 caccctgcat ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat
    14581 catcagcaaa tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa
    14641 agatggcgaa accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg
    14701 taacaagtcg gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga
    14761 ctttaacgat ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag
    14821 cctgctgtac atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg
    14881 cctgaaactg tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa
    14941 ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg
    15001 tgaaatcctc caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt
    15061 gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca
    15121 acagtttggc ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat
    15181 cgccaaactg ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct
    15241 ggaagactac gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga
    15301 cagctatgcg gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga
    15361 agttctgctg ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga
    15421 cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga
    15481 agttgatgag agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa
    15541 agctctgctc ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc
    15601 agcgatcgtt tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc
    15661 ggcgggccag aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact
    15721 ggtgaaacgt gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct
    15781 gctggatcag gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg
    15841 tcgtatgaat cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc
    15901 atttttcttt tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct
    15961 caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa
    16021 atcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc
    16081 ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac
    16141 tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga
    16201 cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg
    16261 cattgctcag gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc
    16321 gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt
    16381 accggacgaa ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg
    16441 tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga
    16501 agaactgact acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata
    16561 ccatcagatg actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac
    16621 caaatacgcg aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa
    16681 aatcctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc
    16741 aggcaatacc tctcacagca attagttctt cttcctcact tttccgctac aattatcaac
    16801 aagttgaatc gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac
    16861 ctgggtacgc ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta
    16921 agcgacagac gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg
    16981 attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac
    17041 ggctcgccgc tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg
    17101 gatacgccag tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat
    17161 gaactcctgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc
    17221 tgttctacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc
    17281 attccgggga tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg
    17341 gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct
    17401 tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc
    17461 acaacgactc gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc
    17521 tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa
    17581 atgctcggag aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat
    17641 tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc
    17701 gggaaaaaat atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg
    17761 gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga
    17821 agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct
    17881 gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctagta aagcagacgg
    17941 ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga
    18001 gtgagatcat tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa
    18061 tacgcctctt catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac
    18121 agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca
    18181 attttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg
    18241 gcacctgcgg aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc
    18301 tgctggtgga aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct
    18361 tcaggtgaaa gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg
    18421 cgcatgatgc gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa
    18481 aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat
    18541 tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg
    18601 cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt
    18661 aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca
    18721 ggtagtttgt tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac
    18781 cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct
    18841 cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa
    18901 tttcccggta aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt
    18961 cagcaattcc agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg
    19021 ctggtcgata ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc
    19081 gggcattcac tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag
    19141 aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac
    19201 tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt
    19261 ggcagttatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta
    19321 caaggcgtgg atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt
    19381 acctttgcta tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat
    19441 gtgattgccg gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt
    19501 gaacccatgc cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg
    19561 gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa
    19621 ttcctcaaag accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc
    19681 ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg
    19741 tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt
    19801 aaaacccagc atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc
    19861 cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg
    19921 tacatgggcg ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca
    19981 gcgttgctgc atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc
    20041 aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac
    20101 cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag
    20161 cgtgaagaca gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc
    20221 agataaggcg ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg
    20281 cgtcttatca tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt
    20341 caccacttca cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg
    20401 ttcacccatc actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata
    20461 atgggcgcgg gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc
    20521 acccgcttca taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc
    20581 cagttgcata atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg
    20641 cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag
    20701 caatttctcc cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca
    20761 aatatccact gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag
    20821 cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat
    20881 cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc
    20941 cattcccagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat
    21001 ggttaatgcc gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga
    21061 tttaccaaac agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
    21121 taacggatca aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa
    21181 tggcaacgta tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc
    21241 aaagaacgca ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc
    21301 taacgccagc gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg
    21361 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
    21421 caccttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg
    21481 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc
    21541 cacaatcaac caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc
    21601 aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc
    21661 ggtcattaac gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc
    21721 tatctgggcg atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga
    21781 aaaatgcaaa ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc
    21841 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
    21901 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
    21961 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
    22021 tcctggtggt tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg
    22081 acaatttcat tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc
    22141 tgcaaaaaaa gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
    22201 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
    22261 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
    22321 gactaactcg atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc
    22381 catgccgaaa gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag
    22441 ctcctgagcg tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac
    22501 cgttaaaacc cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac
    22561 gccgaggata gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc
    22621 gatcaccgta cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg
    22681 gatagataat ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat
    22741 atagaaatat ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt
    22801 gcgcggcagt ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc
    22861 cgagtaccaa cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa
    22921 ccaggcaacg ttgcctttgc cataaggctc gataatcacc gccgccagca agggtcccag
    22981 ggaactgcca aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc
    23041 ggaagccata cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac
    23101 cagcgccgct gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc
    23161 acttaaggta aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca
    23221 gtagccgacc actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc
    23281 aatctgcata aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag
    23341 cgattggatc atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga
    23401 tgttcgggcc ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat
    23461 tgccatagga aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta
    23521 ttacccgact aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa
    23581 tttctaatct ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgttgcgtc
    23641 atactatttt tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg
    23701 gggcgtggcg ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta
    23761 tgagcaggat aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt
    23821 ttggcgcaat gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat
    23881 ccgcaaagag gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa
    23941 cactggcgtg cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct
    24001 ggtgggctat gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt
    24061 acgccagcag gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag
    24121 tactggcgag cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc
    24181 cgttattggg ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga
    24241 tatcgaattt cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac
    24301 agaaaagcca gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca
    24361 cggctctaac gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc
    24421 gatgatcgtc ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca
    24481 ggtcgattac gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt
    24541 gcaggcgcat gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga
    24601 aatgaaaatg gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga
    24661 agacgggaaa agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat
    24721 ctcgctgtta tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa
    24781 aaccaatggt cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg
    24841 gcggttgatt ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg
    24901 aggcggaatt cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt
    24961 gcagccattc ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta
    25021 cctgaccgcc gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt
    25081 tagctttgtg gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga
    25141 tccggcgaaa acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata
    25201 tccgcgcctt gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct
    25261 gaaagcgtat atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga
    25321 ggtgagctgg cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc
    25381 ggcgggcgat atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt
    25441 cgatatccag tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg
    25501 cgtcgataat cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt
    25561 atcccgtcga acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg
    25621 cttttgctac ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt
    25681 gtttcatatc accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac
    25741 ctaatttttt gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat
    25801 ggatttgaaa cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt
    25861 tcctgcctta aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag
    25921 tcatcttgcc taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt
    25981 cgcttaaagt gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg
    26041 atattggcca tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag
    26101 tgcaccgacc gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc
    26161 gagatttcgc tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt
    26221 tatatcatta ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg
    26281 tgcgttggta tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag
    26341 ctggagcaac atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg
    26401 ttgcttcgcc gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt
    26461 ttgtgcctgc gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag
    26521 tgagggagaa atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct
    26581 aatgcgctct tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga
    26641 aacatccgcc tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg
    26701 caggggagcc atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt
    26761 gccggggagg gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca
    26821 aagcgttttt acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag
    26881 cgcgcggtga gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc
    26941 agtatgttct tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa
    27001 caagaaaacc gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt
    27061 tgaaaatcaa tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga
    27121 tcaataacaa tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata
    27181 ccacagtcga ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca
    27241 acatgcgcac ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc
    27301 gtgaagagcg tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg
    27361 ataaggcgtt cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg
    27421 tcttatcagg cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca
    27481 tccggcagtc atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg
    27541 tacttattcc ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga
    27601 cgagagcgcc attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca
    27661 caaaataccg gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt
    27721 ctgcttcatg ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag
    27781 gctatggcgc atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat
    27841 cgcaatgccg acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat
    27901 tgccacctga cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag
    27961 cactccggca atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg
    28021 gttatccccg gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc
    28081 cacgctatca ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat
    28141 cgccagcagc acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc
    28201 tttagtatcg acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc
    28261 ttctgcttca ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag
    28321 ctgcatatcg cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc
    28381 cagcgccgcc gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc
    28441 gacaacctgc ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag
    28501 tgtactggcg cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg
    28561 cccgacgccg gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat
    28621 aatcagcacc gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa
    28681 ataccagatt gccgcactga caagcgcaat aaccactact accggcacaa atatggctga
    28741 gattttatcc gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg
    28801 aatgatcctt gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc
    28861 gtcctgtacc actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg
    28921 ttcgcccgtc agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac
    28981 cggcacgcga tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg
    29041 cacgcttttt tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa
    29101 tttctccagt gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt
    29161 gatcagaccg ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa
    29221 ccactgcggc cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc
    29281 caccagcgta tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa
    29341 atggccgctg gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct
    29401 gcggttgtcg gcggtgacta tcatgttatc gccgatcatc ccccagacca tcaccgggat
    29461 acccaccgcc agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt
    29521 ttcttgctgg cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc
    29581 tttttccacc gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg
    29641 ctccgccagg tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac
    29701 gcgggtgaca cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc
    29761 atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga
    29821 tgactccgcc agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt
    29881 gatggtttcg atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga
    29941 cacatccgct tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca
    30001 gtgaccgcag gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt
    30061 taagacagtt ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga
    30121 aattaataaa ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc
    30181 aggattagac atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc
    30241 aggatgcctg aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt
    30301 taacaaaaga tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa
    30361 tttcactcac ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca
    30421 ggtcaactgg cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac
    30481 agtgattacc gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta
    30541 gaagatgtcg gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc
    30601 tttaactcag ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat
    30661 gctggcgcta ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag
    30721 cgaattttac atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc
    30781 aaccagtcgg aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc
    30841 gccggatatc tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct
    30901 acgctcatca atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg
    30961 aatccgttga cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa
    31021 atgatgatgg aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg
    31081 ggcaaaagcg gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc
    31141 gcgttctcac caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca
    31201 tcggtcgcta agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag
    31261 gtaataacgg taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa
    31321 tggtgggggc ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga
    31381 cctggggcgc ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc
    31441 gtctgggggc gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag
    31501 gcaacggcgt cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca
    31561 tcgccatggt tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc
    31621 aggaggagca ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca
    31681 actccttaag caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga
    31741 tgatcctgtt attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg
    31801 tctctgcgcc ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct
    31861 atgcgggctt tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta
    31921 tgccacgggc gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc
    31981 tggttttgct tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag
    32041 cgcaggctgc ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt
    32101 tactggcgac ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca
    32161 acatgggcag cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta
    32221 cctggggcaa tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag
    32281 actcactcgc cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg
    32341 tggcgatccg cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt
    32401 tggtgatgtt gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg
    32461 cgttgatatg gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc
    32521 gtaataaaac cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg
    32581 tcgatattcg cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc
    32641 acaggaggcg ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc
    32701 agcaaagcca ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa
    32761 aacggttatc gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca
    32821 cggcaggtgg gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg
    32881 cagcggcaca gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga
    32941 cacattgagg agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct
    33001 ggcgatgaca gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg
    33061 gcagggtgat taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac
    33121 ttcaacatgc gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc
    33181 gacgcgcata tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat
    33241 cagctgctgc ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg
    33301 cgacaaccat ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc
    33361 ttgccactcc caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac
    33421 gccgctccac aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc
    33481 gagactgagc cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt
    33541 ttgttggcgc tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg
    33601 gcctctaatg gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct
    33661 tcggtgtatt tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct
    33721 tcagacacca ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc
    33781 tgtaaaaacg ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct
    33841 tcggctttga ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg
    33901 gtacgttccg ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg
    33961 tcgcgaattt caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata
    34021 cgcagcaggc gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa
    34081 cccaacacgg tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg
    34141 acttcatagg cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta
    34201 acgttggcgt tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc
    34261 atattgatct tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt
    34321 aacgttttgg tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt
    34381 ttgacgcccg cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc
    34441 atcggaaaaa cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa
    34501 attatacctg attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt
    34561 agtaaaaatc agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa
    34621 aacgtaggat atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt
    34681 gctggcgaat ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa
    34741 atagttgctt cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc
    34801 agcacactaa aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg
    34861 ctgtttggcg atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag
    34921 cctgacccgg ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg
    34981 acgaagaata tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac
    35041 ctgcaattta tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt
    35101 aataaacata acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg
    35161 ctgtgggtga cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa
    35221 ccgcatgccg gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc
    35281 attagcactg gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact
    35341 ggcgctggag aaagatattc tatggagcgt cgggcgagcg ataattcagc tgattattgt
    35401 cggctatgtg ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt
    35461 gttatttatc tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa
    35521 agcttttatc tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct
    35581 gattctctca ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat
    35641 tgccggtaac gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat
    35701 tagcgaacag caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc
    35761 agcgatattg attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa
    35821 aacggttggc ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga
    35881 tccggtgaag gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag
    35941 cttgtcgacc attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca
    36001 gttggtggtg actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc
    36061 ggcgttgtgg ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg
    36121 gcgatacttc gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt
    36181 tttacgcgtc tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc
    36241 cagcgcctct tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc
    36301 ggcatcttct ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc
    36361 cgcctgcttc agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg
    36421 caacggaatg gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt
    36481 ttctgccagc agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag
    36541 caatggcagg gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt
    36601 cagctcttct tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg
    36661 cggcccctgg aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc
    36721 gcgcagacca aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat
    36781 aaattgcccg ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg
    36841 ctggctacgt tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac
    36901 ctgttgcagg ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc
    36961 tgttgtcgat attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa
    37021 tattttgtcc atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac
    37081 cagcgttacc ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc
    37141 gaccaccgct tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt
    37201 atcactttgc gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat
    37261 caggctgact ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc
    37321 cagcgcatat ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac
    37381 cgatgatgtc atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat
    37441 ggtgagctgg tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct
    37501 gatggtggaa agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca
    37561 attatcggtc agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag
    37621 atcgatcaac acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg
    37681 gcaacctgcc agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat
    37741 tccactggaa catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc
    37801 cgttgcttta tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa
    37861 taaacggctg ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga
    37921 ggtagacctc ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg
    37981 ggtaaatggc gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt
    38041 gcattaacaa tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt
    38101 gctcggtttg ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac
    38161 ccaacgcggc tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt
    38221 gtcgccgctg atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa
    38281 ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat
    38341 caataacgtg tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa
    38401 atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag
    38461 catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca
    38521 ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat
    38581 gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa
    38641 gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt
    38701 cctaccctta acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc
    38761 gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg
    38821 gatcatcttc cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc
    38881 tttaatggtc gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag
    38941 acgggcgata aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg
    39001 attatggtga cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg
    39061 aacgggcagt tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc
    39121 cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg
    39181 gcaatatcag cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg
    39241 gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa
    39301 agcgcggcct gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca
    39361 caccgcagct ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcggtctgc
    39421 aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga
    39481 tggcactgct taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga
    39541 ttgccggaga agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc
    39601 gtctgatgat gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg
    39661 tcacctggcg ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt
    39721 tgttacctca gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc
    39781 tggggcgatc gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc
    39841 tgctggcagt ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc
    39901 tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg
    39961 gtcagtggtt gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt
    40021 tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca
    40081 gcctctggcc gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc
    40141 tacgaccata ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg
    40201 tcgcgaacgt ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc
    40261 tgctcgccgg attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag
    40321 tactggcttt gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga
    40381 cgctgaaatc gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa
    40441 cgttaagcca gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc
    40501 tgcgtggcga tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact
    40561 ttttaattaa catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc
    40621 atatagtccc ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata
    40681 agccgacaga aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa
    40741 atacgcggcc cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag
    40801 tgtcgatgga agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt
    40861 ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg
    40921 aaagtctgcg gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc
    40981 agagctggct taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc
    41041 gccagttccc gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg
    41101 tgctggagca ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta
    41161 tgttgctgtt gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg
    41221 tatggcgcac actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc
    41281 gccatgcttg ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca
    41341 gtgttgcagg cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt
    41401 ctgccgtgca gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg
    41461 gttaagggta aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg
    41521 atgcgctacg cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg
    41581 taggccggat aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa
    41641 tatgaaacca actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt
    41701 agctacgttt cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg
    41761 ccttaagaac gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag
    41821 aaagacaatc acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga
    41881 tgaggactgt ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag
    41941 gacttaaaag tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc
    42001 gggattgtat cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt
    42061 gctagatgag gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa
    42121 aaaaaccgac tgtcatgtcg gtttttttat atctgtattc tggtgcaacc attttgttct
    42181 cacgacaatc gccgtttcag aataccgccc atcactgcgc cttaacccat accgccactt
    42241 ccgcccactc accgcgaaag acaacttttt ccgctttttt ctcaagctga tagcgataca
    42301 ttgggtcgta atattcttta agtaacggca ccagccaggc cagatgaccg tcggtgctgc
    42361 cggtggtgag ttgcgttgtc agcgccgtat ccagctgggc agcgagttcg ttatagcgct
    42421 gtaaccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg caatattcct
    42481 gccagccctg ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc aagaaatact
    42541 cttcattcag gcgttcaaga cggatctcaa acggatcttc taccaccgca atcgccgcct
    42601 gagtcattcg ctcgcgcaga cattccggca ggtgattcga accgatcatc cggctttcat
    42661 cttccagcac ccacaggcgc aaatcctgac gggcgtcggt ttttagcatt tcggcagcca
    42721 gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca aacgccgaac
    42781 cgcgatgacg cgccagccct tccagatcaa caccgtttgg ctgttgttgc actaatagcg
    42841 ttttaccgct gccggtacaa ccgccaatca gcactatcgg tttttgtgcc agttcaatgg
    42901 tcgcctgaat cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc ggataatcaa
    42961 tccccgcttc atgcagccag cgttgcacaa tatgtgaacg ttgaccgcca cgggcgcagc
    43021 agagaatacc ttgcggattt tgcaggcacg ctgcccgcca ggcgtacata cgctgctgac
    43081 gaatttcacc cgccaccagt ttatgcccca gcgccagcgc tgcgtctgag ccttgctgtt
    43141 tatagcaggt gccaacggag gcgcgttcat cgttatccat taacggcaga ttgatagcgg
    43201 cgggcattgc gccttgctca aactcgatag gggcgcgaac atcaataatg ggcgtatcag
    43261 caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac
    43321 ctcaaatcag cttgcgcgct attttacgcg ccaacgcgca aggaaacttg atttttaact
    43381 gcgtggggtg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgtgtt gggtaaacaa
    43441 ggtcacaata tcttctaccg ctttgcagct gccaaatttg cgccatgcca gactcaatgg
    43501 cgaaggaggg cgcatcgttg ggattacccg gctgaccagt tgttgattat cgatcattga
    43561 ctggcaaagt gattttggca aaaaaccaat gccaacaccc gccagatggg cggcgatttt
    43621 cgtttccata tcgggaacaa taatctcttt ttgccccggc aatcgccagg cgacgcgttt
    43681 ggttaaggtg ccggcgctgt cttcaatatt gaccgccgga aagcgacgca actgcgcttc
    43741 tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa atcgccattg
    43801 caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcagttc ccgtgacccc
    43861 gatagccagc gaaaaacctt cgtacaatag cgagtcccag acgcccatat agatttgtcg
    43921 ggagatgtga aactgggtaa aggggtaacg ctcattcagc cacgccagca actgggcgac
    43981 ggcctggggg ttatagagca ggttgttgat gacaatattc acctggcgtt ccacgccatc
    44041 attcacctgt tgcagctcgc tgggcatact ttccagccag ctcagccagt ctctggcctg
    44101 ggaaagtaga tgctcgccag ccgctgtcaa cgttacgcta cgagtcgtac ggaaaaacag
    44161 cgctactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt
    44221 acataatcgt tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg
    44281 caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag ttgcgtgatt
    44341 ttcttatcct ctgatttatc agtattttta catgataatc ctgttcaatt tgtggactaa
    44401 atctagtttt ggaaaaacat tccaactttt gtattaatgt tgttctctta aggttttaga
    44461 ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat tatgttacaa
    44521 ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta gtgcttatgg
    44581 cgacgtaatc gaaacgcagc aacgggattt tttccatatt aacaatggcc tggtggagcg
    44641 ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta tcagcattaa
    44701 ccgcgcgcaa ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc atccgctggg
    44761 cattcaggcc tttatcccaa tgaaaggtga agtgtttgtg gtggtcgtgg cgttaggcga
    44821 cgacaaacca gacctgtcaa cgctgcgggc gtttatcacc aacggtgaac agggggtgaa
    44881 ttaccatcgt aacgtctggc atcacccact tttcgcctgg cagcgcgtca ccgattttct
    44941 gaccatcgat cgcggcggca gtgacaactg tgatgttgaa agtattcctg aacaggaact
    45001 ctgttttgcg tgacgcctgc aaccgacttg cataagataa actaattgtt cattgtttat
    45061 gctcacttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg cggcaggcca
    45121 ggacaggcgg agcctgtggc acagaagggc gcacaggcgt tagagcgggg aattgcgatt
    45181 ctgcaatatc tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc tctcaatctg
    45241 gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaagcagc ggattttgtc
    45301 tatcaggaca gtcaattagg ctggtggcat ataggattag gtgtctttaa cgtcggtgcg
    45361 gcgtacatcc ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg ccgcctgatg
    45421 ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc ggtattaatt
    45481 ggtcagttag agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag tcgtctgccg
    45541 ctgcatgctt ccggtgcggg caaagcgctg ctttatccgc tggcggaaga ggagttgatg
    45601 agcatcattc tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt ggatatgccc
    45661 accttgctga aggacctgga acaagcgcgt gaactgggct ataccgtaga taaagaagag
    45721 catgttgtag gtctgaattg catagcttca gcaatttacg atgatgtcgg tagagttgtt
    45781 gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt tgtcagtcag
    45841 ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact gaaagcacat
    45901 ccatagtgtc tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa tttgaaagtt
    45961 ggaaaatttt tccaactaat agaggtagga ataaaatggc aaaaatgaga gccgttgacg
    46021 cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt ccgggagctg
    46081 caattaatcc gttctactca gcgatgcgta agcacggcgg tattcgtcac attctggcgc
    46141 gtcatgtgga aggtgtttcg cacatggcgg aaggttatac ccgcgcaacg gcagggaata
    46201 tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc gcgctctatt
    46261 ccgcttctgc tgattccatt cctattctgt gcattaccgg ccaggcaccg cgcgcccgtc
    46321 tgcataaaga agattttcag gccgtagata ttgaagcaat tgctaaaccg gtcagcaaaa
    46381 tggcggttac agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag gcatttcacc
    46441 tgatgcgttc tggtcgtccg ggtccggtac tggtggattt accgttcgac gttcaggttg
    46501 cggaaatcga gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa cctgctgcca
    46561 gccgtatgca gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt ccggtgattg
    46621 ttgccggggg cggggtaatc aatgctgacg cagctgcact gttacaacag tttgctgaac
    46681 tgaccagcgt tccggtgatc ccaacgctga tgggctgggg ctgtatcccg gacgatcatg
    46741 aactgatggc cgggatggtc ggtctgcaaa ccgcgcatcg ttacggtaac gcaacgttgc
    46801 tggcgtccga catggtgttt ggtatcggta accgttttgc taaccgtcat accggttcgg
    46861 tagagaaata caccgaaggg cgcaaaatcg ttcatattga tattgagccg acgcaaattg
    46921 gtcgcgtgct gtgtccggat ctcggcattg tctctgatgc taaagcggcg ctgacactgc
    46981 tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt aaagaatggg
    47041 tcgccgactg ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc gacaacgtgc
    47101 cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc gatgtttgtt
    47161 atgtcaccac tattggtctg tcacaaatcg ctgcggcaca aatgctgcat gtctttaaag
    47221 accgccactg gatcaactgt ggtcaggctg gtccgttagg ctggacgatt ccggcggcgc
    47281 tggggggttg tgccgctgat ccgaaacgca atgtggtggc gatttctggc gactttgact
    47341 tccagttcct gattgaagag ttagccgttg gcgcgcagtt caacattccg tacatccatg
    47401 tgctggtgaa taacgcctat ctcggcctga ttcgtcagtc acaacgcgct tttgacatgg
    47461 actactgcgt gcaactcgct ttcgagaata tcaactccag tgaagtgaat ggctacggtg
    47521 ttgaccacgt aaaagtagcg gaaggtttag gttgtaaagc tattcgggtc ttcaaaccgg
    47581 aagatattgc gccagccttt gaacaggcga aagccttaat ggcgcaatat cgggtaccgg
    47641 tagtcgtgga agttattctc gagcgtgtga ccaatatttc gatgggcagc gaactggata
    47701 acgtcatgga atttgaagat atcgccgata acgcagcgga tgcaccgact gaaacctgct
    47761 tcatgcacta tgaataaggg agataaatga tgttacgttt ctctgctaat ttatcgatgt
    47821 tatttggtga atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt ggttttcgcg
    47881 gcgttgaatt tatgtttcct tatgactacg acattgaaga attaaaacag gtactggcga
    47941 gtaataaact cgaacatacg ctgcacaatt taccggcagg tgactgggcg gcgggcgagc
    48001 gcggtattgc ctgtattcct ggccgtgaag aagagtttcg ggatggcgta gcagcagcga
    48061 tt
//
LOCUS       DBBBSB010000018        47956 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.018, whole genome shotgun sequence.
ACCESSION   DBBBSB010000018 DBBBSB010000000
VERSION     DBBBSB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47956
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.018"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..79
                     /locus_tag="ACIUS0_000983"
     CDS             <1..79
                     /locus_tag="ACIUS0_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218839.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACIUS0_000984"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACIUS0_000984"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGB0218840.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACIUS0_000985"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACIUS0_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGB0218841.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACIUS0_000986"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACIUS0_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGB0218842.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACIUS0_000987"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACIUS0_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGB0218843.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACIUS0_000988"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACIUS0_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGB0218844.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACIUS0_000989"
     CDS             5377..5835
                     /locus_tag="ACIUS0_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGB0218845.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACIUS0_000990"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACIUS0_000990"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGB0218846.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACIUS0_000991"
     tRNA            6887..6971
                     /locus_tag="ACIUS0_000991"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACIUS0_000992"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACIUS0_000992"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACIUS0_000993"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACIUS0_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGB0218847.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACIUS0_000994"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACIUS0_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGB0218848.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACIUS0_000995"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACIUS0_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGB0218849.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACIUS0_000996"
     CDS             complement(10252..13995)
                     /locus_tag="ACIUS0_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGB0218850.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACIUS0_000997"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="ACIUS0_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGB0218851.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="ACIUS0_000998"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="ACIUS0_000998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGB0218852.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="ACIUS0_000999"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="ACIUS0_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGB0218853.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACIUS0_001000"
                     /gene_synonym="iatA"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="ACIUS0_001000"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGB0218854.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="ACIUS0_001001"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="ACIUS0_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGB0218855.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACIUS0_001002"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="ACIUS0_001002"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGB0218856.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACIUS0_001003"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="ACIUS0_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGB0218857.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="ACIUS0_001004"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="ACIUS0_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGB0218858.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="ACIUS0_001005"
     CDS             23392..23499
                     /locus_tag="ACIUS0_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGB0218859.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACIUS0_001006"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="ACIUS0_001006"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGB0218860.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACIUS0_001007"
     CDS             complement(24538..25347)
                     /gene="sirB1"
                     /locus_tag="ACIUS0_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGB0218861.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACIUS0_001008"
     CDS             complement(25351..25743)
                     /gene="sirB2"
                     /locus_tag="ACIUS0_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGB0218862.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACIUS0_001009"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="ACIUS0_001009"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGB0218863.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACIUS0_001010"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="ACIUS0_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGB0218864.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACIUS0_001011"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="ACIUS0_001011"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGB0218865.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="ACIUS0_001012"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="ACIUS0_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGB0218866.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="ACIUS0_001013"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="ACIUS0_001013"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGB0218867.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="ACIUS0_001014"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="ACIUS0_001014"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGB0218868.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="ACIUS0_001015"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="ACIUS0_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGB0218869.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACIUS0_001016"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="ACIUS0_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGB0218870.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="ACIUS0_001017"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="ACIUS0_001017"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGB0218871.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="ACIUS0_001018"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="ACIUS0_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGB0218872.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="ACIUS0_001019"
     CDS             36790..39657
                     /locus_tag="ACIUS0_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGB0218873.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACIUS0_001020"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="ACIUS0_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGB0218874.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="ACIUS0_001021"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="ACIUS0_001021"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGB0218875.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="ACIUS0_001022"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="ACIUS0_001022"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGB0218876.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="ACIUS0_001023"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="ACIUS0_001023"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGB0218877.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="ACIUS0_001024"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="ACIUS0_001024"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGB0218878.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACIUS0_001025"
                     /pseudo
     CDS             complement(47142..47581)
                     /gene="ycgY"
                     /locus_tag="ACIUS0_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
BASE COUNT        12040 a        12343 c        11744 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtgaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaatg
//
LOCUS       DBBBSB010000019        47485 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.019, whole genome shotgun sequence.
ACCESSION   DBBBSB010000019 DBBBSB010000000
VERSION     DBBBSB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.019"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="ACIUS0_001026"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="ACIUS0_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            1316..1441
                     /gene="timP"
                     /locus_tag="ACIUS0_001027"
     CDS             1316..1441
                     /gene="timP"
                     /locus_tag="ACIUS0_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGB0218879.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACIUS0_001028"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="ACIUS0_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGB0218880.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACIUS0_001029"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="ACIUS0_001029"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGB0218881.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACIUS0_001030"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="ACIUS0_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGB0218882.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACIUS0_001031"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="ACIUS0_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGB0218883.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACIUS0_001032"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="ACIUS0_001032"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGB0218884.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACIUS0_001033"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="ACIUS0_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGB0218885.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACIUS0_001034"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="ACIUS0_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGB0218886.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACIUS0_001035"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="ACIUS0_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGB0218887.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACIUS0_001036"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="ACIUS0_001036"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGB0218888.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACIUS0_001037"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="ACIUS0_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGB0218889.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACIUS0_001038"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="ACIUS0_001038"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGB0218890.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="ACIUS0_001039"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="ACIUS0_001039"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGB0218891.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="ACIUS0_001040"
     CDS             11405..12259
                     /locus_tag="ACIUS0_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGB0218892.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="ACIUS0_001041"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="ACIUS0_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGB0218893.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="ACIUS0_001042"
     CDS             complement(13722..14861)
                     /locus_tag="ACIUS0_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGB0218894.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACIUS0_001043"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="ACIUS0_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGB0218895.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /gene="hcaE"
                     /locus_tag="ACIUS0_001044"
     CDS             16047..17408
                     /gene="hcaE"
                     /locus_tag="ACIUS0_001044"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGB0218896.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /gene="hcaF"
                     /locus_tag="ACIUS0_001045"
     CDS             17405..17923
                     /gene="hcaF"
                     /locus_tag="ACIUS0_001045"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGB0218897.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="ACIUS0_001046"
     CDS             17923..18243
                     /locus_tag="ACIUS0_001046"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGB0218898.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="ACIUS0_001047"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="ACIUS0_001047"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGB0218899.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20284
                     /gene="hcaD"
                     /locus_tag="ACIUS0_001048"
                     /pseudo
     CDS             19062..20284
                     /gene="hcaD"
                     /locus_tag="ACIUS0_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20381..20803
                     /locus_tag="ACIUS0_001049"
     CDS             20381..20803
                     /locus_tag="ACIUS0_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGB0218900.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20851..21723)
                     /locus_tag="ACIUS0_001050"
     CDS             complement(20851..21723)
                     /locus_tag="ACIUS0_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGB0218901.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21735..22829)
                     /locus_tag="ACIUS0_001051"
     CDS             complement(21735..22829)
                     /locus_tag="ACIUS0_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGB0218902.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22862..23860)
                     /locus_tag="ACIUS0_001052"
     CDS             complement(22862..23860)
                     /locus_tag="ACIUS0_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0218903.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23885..25396)
                     /locus_tag="ACIUS0_001053"
     CDS             complement(23885..25396)
                     /locus_tag="ACIUS0_001053"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGB0218904.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25419..26401)
                     /locus_tag="ACIUS0_001054"
                     /pseudo
     CDS             complement(25419..26401)
                     /locus_tag="ACIUS0_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26498..29779)
                     /locus_tag="ACIUS0_001055"
                     /pseudo
     CDS             complement(26498..29779)
                     /locus_tag="ACIUS0_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29897..31090
                     /locus_tag="ACIUS0_001056"
     CDS             29897..31090
                     /locus_tag="ACIUS0_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGB0218905.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31288..32541)
                     /gene="glyA"
                     /locus_tag="ACIUS0_001057"
     CDS             complement(31288..32541)
                     /gene="glyA"
                     /locus_tag="ACIUS0_001057"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGB0218906.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32868..34058
                     /gene="hmpA"
                     /locus_tag="ACIUS0_001058"
     CDS             32868..34058
                     /gene="hmpA"
                     /locus_tag="ACIUS0_001058"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGB0218907.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34103..34441)
                     /gene="glnB"
                     /locus_tag="ACIUS0_001059"
     CDS             complement(34103..34441)
                     /gene="glnB"
                     /locus_tag="ACIUS0_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGB0218908.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34502..35836)
                     /gene="glrR"
                     /locus_tag="ACIUS0_001060"
     CDS             complement(34502..35836)
                     /gene="glrR"
                     /locus_tag="ACIUS0_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGB0218909.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35826..36539)
                     /gene="qseG"
                     /locus_tag="ACIUS0_001061"
     CDS             complement(35826..36539)
                     /gene="qseG"
                     /locus_tag="ACIUS0_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGB0218910.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36704..38131)
                     /gene="qseE"
                     /locus_tag="ACIUS0_001062"
     CDS             complement(36704..38131)
                     /gene="qseE"
                     /locus_tag="ACIUS0_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGB0218911.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38689..42576)
                     /gene="purL"
                     /locus_tag="ACIUS0_001063"
                     /gene_synonym="purI"
     CDS             complement(38689..42576)
                     /gene="purL"
                     /locus_tag="ACIUS0_001063"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGB0218912.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42835..44391
                     /gene="mltF"
                     /locus_tag="ACIUS0_001064"
     CDS             42835..44391
                     /gene="mltF"
                     /locus_tag="ACIUS0_001064"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGB0218913.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44388..44891)
                     /gene="tadA"
                     /locus_tag="ACIUS0_001065"
     CDS             complement(44388..44891)
                     /gene="tadA"
                     /locus_tag="ACIUS0_001065"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGB0218914.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44949..45584)
                     /gene="yfhb"
                     /locus_tag="ACIUS0_001066"
     CDS             complement(44949..45584)
                     /gene="yfhb"
                     /locus_tag="ACIUS0_001066"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGB0218915.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45793..46641
                     /locus_tag="ACIUS0_001067"
     CDS             45793..46641
                     /locus_tag="ACIUS0_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGB0218916.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46980..47485)
                     /locus_tag="ACIUS0_001068"
                     /pseudo
     CDS             complement(46980..47485)
                     /locus_tag="ACIUS0_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11323 a        13016 c        11948 g        11198 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt cacagccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gtgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat
    19621 gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg
    19681 tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact
    19741 gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta ttggtatcag
    19801 cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca ttgtcattga
    19861 tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg
    19921 tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca
    19981 aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc cgtggttctg
    20041 gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    20101 ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct
    20161 tatcggtgcg gtcacgctga atcagggccg tgagattcgc ccaattcgca aatggatcca
    20221 gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact
    20281 gtaatcagga taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg
    20341 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt
    20401 tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat
    20461 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    20521 ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    20581 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt
    20641 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt
    20701 tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt
    20761 ggcaattacc ggaccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg
    20821 gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca acgacatctc
    20881 tgaggttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg
    20941 ctcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc
    21001 aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat
    21061 gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    21121 attgttcacc cactgacgcg gcaacggcgc gagctggtta aaatccagtt cctgcggtag
    21181 ctgctcgcaa aactcacccg ccagccactg ctcccgttcc agccagtaac cgctcgcctg
    21241 cgcctgaatc cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg taccaaatgg
    21301 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    21361 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    21421 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    21481 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    21541 acggttgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc
    21601 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat
    21661 tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt
    21721 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    21781 atcagcacat aggcatcacc tgcctgttct agcgagaagc gatgggtaat ggcgttacgc
    21841 ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc
    21901 acccaggagc cgataatccg ccgttgatgg tgcatcagat cagcgctgac ctcgaattcc
    21961 acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc
    22021 agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta
    22081 agttcagcaa ttatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcccc
    22141 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt
    22201 gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc
    22261 agcacgttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    22321 ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    22381 ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    22441 tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag
    22501 ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc
    22561 atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta
    22621 tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa
    22681 atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact
    22741 tcccgcagat cgacggttga atttcctagt aaataagcag ccagcatcgt tttcataaat
    22801 ccctcattaa caatacgatt aattttcatc cctgcagcac ccgcgccagg ggcgttaagg
    22861 tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca
    22921 cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac
    22981 cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac
    23041 cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    23101 ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg gtcaccgccg
    23161 ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca
    23221 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc
    23281 ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac
    23341 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg
    23401 cgttggtcat aaacagcccc attccgcgca gagcgctcca cagccccagt gtggcaacga
    23461 aacttggtac gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcgccta
    23521 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    23581 ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    23641 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    23701 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    23761 caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga
    23821 cgctcttgcc ctgcggcaat ggtaatgacg aagcatacat cttcaggttc ctcggtaaaa
    23881 ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa
    23941 actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaatt
    24001 cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa
    24061 tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca
    24121 aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag
    24181 aaagcgtgcc gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct
    24241 cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc
    24301 gattggtcag cactgtattt tcgtcaacgc ctagccaggg aatgatcccc gcttctttgc
    24361 ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga
    24421 ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct
    24481 tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac
    24541 gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta
    24601 cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga tcacgcccta
    24661 gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct
    24721 gaccatcgcg cataacggtg gcataggagg caatgcggcg gatttcttcc atccggtggc
    24781 tgacataaat caccgccacg cccagtgccg acatcttttt caccgcactg atcaccagtt
    24841 caacttctgc actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc
    24901 ccttcatcac ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc gttgaaacaa
    24961 gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca
    25021 tttgcaggta atcaatcatg ccgttgcggc gaggccattg accaaggcag aggttttctg
    25081 ccaccgtcag cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag
    25141 cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat
    25201 caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc
    25261 cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca
    25321 gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct
    25381 ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc
    25441 gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc
    25501 actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg
    25561 cccattttct taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc
    25621 tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga
    25681 ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg
    25741 agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc
    25801 ccttcctggt tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat
    25861 cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc
    25921 tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt ttaccaaatt
    25981 ccagtggatc gccgaccaga tacgccgaga catatttatc gacacccttt tgattaatac
    26041 aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac
    26101 tgccattttc agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat
    26161 caacaaaggt actttctttc gaaatatccc cctgggcgtt agtttcaatt aactgtacct
    26221 gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct
    26281 gggtatcaag gtatattgca ccaatggtca tttctttatc cgcagccctg gcaaatagtg
    26341 cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca
    26401 tagtgcctcc tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg
    26461 gcagcgcgtg ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa
    26521 aaggcatcac agtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt
    26581 cagcgcgagc agactcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca
    26641 ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag
    26701 gttgtgaaac agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact
    26761 gtttcatttc actaaacagt tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca
    26821 ttccttgcca gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg
    26881 tgcgatcgcc ggtcgccgcc agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg
    26941 cgttggcgca tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc
    27001 cttcgcttaa attctccgga taatacagcg cggcatgaag cagttcgctg gcctgctgcg
    27061 gctctctggc atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg
    27121 tgaccttccc ttccccgcct tcccacgggt ggaatttacg cgtggcgaga atgtccgccg
    27181 ctttgtctgc ctgacccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc
    27241 gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc
    27301 cacttaactt atccagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct
    27361 gataagcatt atcaagataa cgcgcggcca gctcataatc gtgtttctta ttccacgcat
    27421 ggatcgctaa cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc
    27481 ataaggcaat ggctttgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc
    27541 gagcaaacca acactcttca atactctcca gcgccgccac ttcttccagc gtattcggga
    27601 aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt
    27661 tcggcagcaa gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca
    27721 gcatctctgc cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcagag
    27781 cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac
    27841 catcgaacca gttcagccac cacagagtgg cgttcagcgg ataatcacgc agcagtttct
    27901 cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaagc
    27961 aaagcacttc ctgattggtt gggcaggcac gaagactttg ttggcaaaaa tccagacccg
    28021 cgtcgaagtt atcattacgc gccgccagtc gtgccagacc ataatagccg cctgctttac
    28081 tattgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac
    28141 gttcgtaagc actggcgcga atcaaacttg cttgtccaca ctgcggtttt ttgttcagcg
    28201 catgtgcacg tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa tctgcgcggt
    28261 tatattccag catcgccagc gccaggttac agcgataatc cagcgggtcc agcgccacgc
    28321 cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac
    28381 cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc gctttggcga
    28441 cgtccggcaa cggcaactcc tgcggctgat gttcctgata actcagtaca atattgccgt
    28501 cggcatcgga gagttcaatg gtcagtctgt caggattgat accgtgcaac acgccctgga
    28561 tggcggtcgc tggtgtcagg gccacggcat catcgagcaa cgcgttgcat ttgccgattt
    28621 cgcggatcgc cagacggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc
    28681 cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc accatgccca
    28741 gagaatgata aggcaggaaa tactgctcga aacgcttctc ttcgtaagca tcaagccagg
    28801 taaaatcagg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat
    28861 tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa ctccactgtt
    28921 ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct tcatcgtgac
    28981 accacgcgcc gacaaaatcg tactgcgatt tttcggccat atatgaggtt ggaacaggca
    29041 catttttata gcgagaaatg tccactccgg cggagtagtc cactttgtag taagtgccgg
    29101 tggcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg
    29161 gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttgacc caccacaaga
    29221 aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagt gccgcccggt
    29281 cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccga
    29341 cccacaccgt ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg
    29401 tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca
    29461 gcatccccac cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca
    29521 ctttatccca tgcgcgatgc acccgaccgc ccagttccgg caggatcatc actttgatgt
    29581 agtcgttttc cagccacacc gcctgctagg atttcagggt tttctgctcg ctcagcgtat
    29641 cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga
    29701 acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc
    29761 acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt
    29821 tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa
    29881 acaaggttta gtgaagatga gagcctgcat taacaatcaa cagattcgcc accataacaa
    29941 atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg
    30001 gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa
    30061 acgggtggtg aatattgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct
    30121 gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcccacca gtattgagtg
    30181 tcaggttgct aatgcttgtt taagtccgaa aggcgaattt gagtatttac agattgatgt
    30241 accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt
    30301 gtggccggac cgcaccatca atctggcgct ggcaatccac gggcaggttg atccggtgac
    30361 cggcgtatcg caaaccatgc cgcaagcgcc gtggacaacg ccagttgagg tgaagtatct
    30421 gctggaagag aagctgggca ttcgggtgct ggtcgataat gactgcgtga tgctggcgct
    30481 ggcggagaaa tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta
    30541 cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag
    30601 cggacagatt ggtcacacca tcgttaatcc ggatggagtc gtctgcgact gtggacgtta
    30661 cggcagcctg gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc gggtatggct
    30721 aaaatcacaa ccggttaata ctcagcttga gcctgaaaaa ctgacaaccg cgcagttaat
    30781 cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc
    30841 cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta
    30901 cggtcgcagt tgtgcctttg gtgagaactg gcttaatact attattcgcc agacaggatt
    30961 taacccgttc gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca
    31021 attaagccgc gcacaacagg tgctgggaat tggctatttg tatgttgagg cgcagttacg
    31081 acagatttga tggcgcgata acgtagaaag gcttcccgaa ggaagccttg atgatcataa
    31141 acgaaaaatt gcctgatgcg ctacgcttat caggcctaca cggagattgc aatatattga
    31201 atttgcaaga ttttgtaggc ctgagaaggt gttcatgccg catccaacat gaacaacgag
    31261 cacattgtca gcaaatcacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc
    31321 gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc
    31381 acacatccag ccagccagtt ctttcgcttc ggcttcttta aagccgcgac gggtaatcgc
    31441 cggagtacct acacgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct
    31501 gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag
    31561 gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg aaaccacttt
    31621 gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga cctgctgctg
    31681 gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac
    31741 gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag
    31801 ctcttcgcta ccacctttcg ccaagatcag gccgccgcgc ggacccgcca gggttttgtg
    31861 agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac
    31921 caggcccgca acgtgcgcca tatcaacgaa cagataagca ccgatgctgt cagcgatttc
    31981 acgcattttc gcccagtcaa ccacgccgga atatgcagag aaaccaccga taatcatttt
    32041 cggcttgtgt tctttggctt gtttttccag atcggcgtaa tcgatatgac cggtagcatc
    32101 gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt
    32161 caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc
    32221 ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc
    32281 gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca
    32341 accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc
    32401 ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc
    32461 ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat
    32521 gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc
    32581 tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca
    32641 ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa
    32701 taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt
    32761 catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata
    32821 agatgcattt gagatacatc aattaagatg caaaaaagga agaccatatg cttgacgctc
    32881 aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa
    32941 ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca
    33001 tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg
    33061 ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc
    33121 acaccagctt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa
    33181 cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg
    33241 gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca
    33301 aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg
    33361 cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc
    33421 cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc
    33481 agtactcttt gactcgcaaa ccggatggca aaggctatcg tatcgcggtg aaacgcgaag
    33541 agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac
    33601 tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa
    33661 tctctaccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag
    33721 gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgaagtt cacgcctttg
    33781 ctgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc
    33841 gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt
    33901 tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg
    33961 ttggcttcat gcagtttacc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca
    34021 ttcattacga atgctttggc ccgcataagg tgctgtaatt tgatgttgcc ggatggaaac
    34081 atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc
    34141 gaatgacccg agccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg
    34201 ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa
    34261 ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc
    34321 ctttctggcg accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg
    34381 ccagtgcttc gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca
    34441 tgctattcct tgaaaaggtt gcctgtcttt tgatctgcta aacgtaacac ataacgccaa
    34501 ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg
    34561 tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg atttgcagca
    34621 gcttacgcaa gtagttgagt tcaaaatgat tacgtgcctc aacaaaggtt ggcagcgccg
    34681 tattttcacc ctccagcgcc tgctccacca gcgcatcact aatcaccgga gatgaggtca
    34741 gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg
    34801 tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg
    34861 ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc tccgccagtg
    34921 ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc
    34981 gcgccatcgc ttttgacaga tcacggtgag tggcagaaat aatccgcaca ttgatatcaa
    35041 tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga
    35101 cctgtaacgg cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg
    35161 cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa
    35221 acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt
    35281 tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc
    35341 tctgaccgtt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca
    35401 aacgcagcat cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg
    35461 gcgcggattg ctccagcgca tcgtcaattg cctgatatag cgcatcttta tcgacaggct
    35521 tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat
    35581 gcgcagtaag aataattact ggcattcccg gctgcacttt ctggatttca gcaaatagct
    35641 gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat
    35701 tcagtacccg taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg
    35761 tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg
    35821 caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg
    35881 tgcggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata
    35941 tcggtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt
    36001 tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg
    36061 cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg
    36121 cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc
    36181 tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag
    36241 ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca
    36301 caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc
    36361 cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct
    36421 tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga
    36481 catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata
    36541 ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga
    36601 cagcgcggag aaaccggggc gtacatatcg tacgtgaaga tttcgagcac tacccggggc
    36661 cgaactgaca aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg
    36721 acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt
    36781 ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca
    36841 ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct
    36901 cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca ccatgtaaac
    36961 tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat
    37021 tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga
    37081 cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca
    37141 ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt
    37201 aatcaagcag ttgttcgatc agtttttgca aattgcagct gctgctatca agaatgctca
    37261 ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc
    37321 cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat
    37381 ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg
    37441 agcgtaactc gctcggtcca cagaacgaaa cgctattgcc cagagaacgc ccttccccca
    37501 gacggttgat catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta
    37561 gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac
    37621 gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct
    37681 gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg
    37741 gaccactgtt gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt
    37801 aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc
    37861 gctggctttg ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc
    37921 tacgctccat ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg
    37981 tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc
    38041 atgccagcac caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta
    38101 atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc
    38161 tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat
    38221 ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat
    38281 cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg
    38341 aaggggctga cataagtcgg tgaatgagcc actgcttact attatgcagt aactgtgcca
    38401 ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgagggat ttatggcttc
    38461 ttcgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact taagataaac
    38521 ttattagatt gcaataaaat caatcattta agtgtcgcca gaaagcgaca cggtaaacca
    38581 cccattgtcg tgatttacag acacaaaaaa gctcccggag ttgggagctt atgatagtgg
    38641 ttggtgctta atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt accccaactg
    38701 cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttccggatg
    38761 ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg
    38821 accactttca gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt tagccgggta
    38881 ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc
    38941 cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc
    39001 aatcggcatt tgcgagccca ccatcccctg caacagcaga gacgggcttt gggttacttc
    39061 aaccaggctg aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac gtggccacaa
    39121 ctcactcccc gggatcagtt cacgcaggtt agacatcatc tggcaaccgt tacatacccc
    39181 cagggccagc gtttgcggac gatggaagaa ggttgcaaac tcatcgcgta cacggtcatt
    39241 gaacaggata gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc
    39301 tccgcacgcg accagggcgt gaaaatcctc cagtcccgta cgtccagtca gcaggtcgct
    39361 catatgcacg tcgatagcat caaagccccc acgttggaaa gctgccgcca tttcaacatg
    39421 cgagttcacg ccctgctcac gcagtacggc aactttcgga cgtgcgccag tggcaatata
    39481 cggtgctgcc acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc
    39541 gtcgttagat ttcgcctgat gctcctgatc ggcacactcc gggttgtcac gcaggcgctg
    39601 catctgccag gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac
    39661 agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg
    39721 gacgcaatcc gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc
    39781 acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc
    39841 cagagaggcg atatccgcgt caatgccaca atgacctgca aaggccattt ccgccagcgt
    39901 taccagcagg cctccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag
    39961 cgcctgaatc gcgtcataga agcctttcag ttggacaaca tcgcgtacat ctgccggttt
    40021 gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt
    40081 gcccaaatca atcagcagca gtgcgttatc ttcggtacaa agctgcggcg tgatggtgtg
    40141 acgtacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc
    40201 gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg
    40261 gatcgtcagg cccagcgccg gacaaagttc ttcacccacg gctttaacgg cttcatacag
    40321 gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg
    40381 tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg
    40441 ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc
    40501 accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca
    40561 cggccccacc atctgatcgc gcgccaccat gccggttacg ctgcggtcac cgatggtcac
    40621 caggaaagtt ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat
    40681 ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt
    40741 catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa
    40801 atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg
    40861 tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag
    40921 cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc
    40981 gcttagaata tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca ccagctccgg
    41041 catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata aacagaattg ggttagcatc
    41101 accaagctgc cagcaacggt cgatcacttc ctggcaacga cgctccatct ccgggttgtc
    41161 gcgctgtacg gaagcaaagt cgaggtcggc atcagactga ccagacgcca tggaagacgc
    41221 cgcgccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc
    41281 gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag
    41341 catgatcggt ttgtgataac cgcgcagctc ttcgccattg tggctgttca ctttttcttc
    41401 ataagtacgg aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa acgccgcacc
    41461 gcccagcggg ccttcggtca tgatgtccag cgcggtaaca atgcgctcag gcttaccgaa
    41521 atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggatacgg agaaaccaac
    41581 caaaccagct ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc
    41641 ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac
    41701 tttcatcagg atgtgcgccg gttcctgatg gaaattgtag cggcccgttt cgtggtcagc
    41761 aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag
    41821 aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg
    41881 ttgttcaccg tcgataatcc agtcggcgtt aaaaattttg tggcggcagt gctcagagtt
    41941 cgcctgagcg aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc
    42001 atcctgcaga tagtcaattt catcttccgc cagagccaag ccaagacgca ggttagcgtc
    42061 gatcagcgcc tgacggccct gccccagcaa atcaacgctt gtgaccggag tcggttgatg
    42121 gtgggcaaac aactgctctg catcatctaa atcaaaaaag accgtttcca tcatgcggtc
    42181 gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata
    42241 gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc
    42301 ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt
    42361 gccttgcggg gcgtggctgg cgagtgccgg accatatttc agcaggcgtt caagttgtgc
    42421 gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat
    42481 attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc
    42541 cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc
    42601 ggtgtaccca agggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac
    42661 cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta
    42721 ataagttgcc atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc
    42781 actattatta cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa
    42841 aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc
    42901 tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg
    42961 ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa
    43021 ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg
    43081 aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc
    43141 gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc
    43201 cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt
    43261 acgctgggca acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtagttaac
    43321 gatctccaga ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa
    43381 aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct
    43441 gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat
    43501 atcaccgatg aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc
    43561 gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa
    43621 gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc
    43681 gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt
    43741 gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact
    43801 tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc
    43861 attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat
    43921 atgatgagta aagtgccgga aagtgtgccg gagaacgaac ggatctggtt tgccctcgct
    43981 gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa
    44041 gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc
    44101 tattacagca agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa
    44161 aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct
    44221 acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc
    44281 aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat
    44341 tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag
    44401 gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc
    44461 aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc
    44521 ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca
    44581 aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca
    44641 agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc
    44701 cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag
    44761 ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca
    44821 tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc aatattcgtg gctaaattcg
    44881 acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat
    44941 gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga
    45001 cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc
    45061 ggcgtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc
    45121 aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc
    45181 cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag
    45241 atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg
    45301 gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg
    45361 ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct
    45421 tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc
    45481 ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt
    45541 ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga
    45601 ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc
    45661 cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca attagcggca
    45721 atggaatata aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc
    45781 gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaagg gcttgcccag
    45841 agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc
    45901 tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa
    45961 aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc
    46021 cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg
    46081 ctggtcggcg aaaaactgat taaagaaaat accgccgcta tgtacgcaac gctaaacgtt
    46141 aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt
    46201 ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag
    46261 attggcttca atgctgccgc actgcgcgat atgcatgcgc tactcgcaac agtacaggcg
    46321 tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac
    46381 ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact
    46441 ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg
    46501 acaaacagtg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc
    46561 attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg
    46621 ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg
    46681 ttttgagagt ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg
    46741 tgaaatcaaa taaaaacaca tttattttca tagtgataca gcaatgtgtt attgaatgtg
    46801 ttgttggatt tatggtgcaa aaaaattttg cataagttca gcaacacaaa gaacaccact
    46861 ttctttaacg tcctccgcgc cctaaaggac ggggctttac ggcgcaaaag gcctgtgaca
    46921 tacaacatta acttttcagt caaccgtagc acgttcttgt atacgccgtg tctgcggttt
    46981 tatataaccc tggctggaaa cctcttatac aaagtcgatt cgccaacatc atagatgatc
    47041 gccaccttct ggcgagaaac tcctgatgca attagtcgcc cggcctgcgc ccattattct
    47101 ggtgaaagtt tgggacggcg accgccaatt cgtccctgtg cgcgagcagc ttccagtcct
    47161 gcttttgttc gttcaacaat cagttcacgt tccatttcag ccagggcacc catcacatgg
    47221 aagaaaaagc gtcccattgg tgtgctggta tcaattgaat cagtcagact acgaaagttg
    47281 atgcctcgtt cgcgcaacgc ctccaccagc acgacaagat gccgcatact gcgccccagc
    47341 cgatccagct tccagacaac cagtgtgtcg cctgccgata atgtcttgag cagttttttc
    47401 agccccggtc tttcggactt tgtaccactt atcttgtctt caaaaatcag ctcgcatcct
    47461 gcacagttca acgcattacg ttgta
//
LOCUS       DBBBSB010000020        44702 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.020, whole genome shotgun sequence.
ACCESSION   DBBBSB010000020 DBBBSB010000000
VERSION     DBBBSB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.020"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACIUS0_001069"
     CDS             complement(27..914)
                     /gene="rpnA"
                     /locus_tag="ACIUS0_001069"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGB0218917.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACIUS0_001070"
     CDS             complement(1117..1353)
                     /gene="feoC"
                     /locus_tag="ACIUS0_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGB0218918.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACIUS0_001071"
     CDS             complement(1353..3674)
                     /gene="feoB"
                     /locus_tag="ACIUS0_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGB0218919.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACIUS0_001072"
     CDS             complement(3691..3918)
                     /gene="feoA"
                     /locus_tag="ACIUS0_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGB0218920.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4356..6677)
                     /locus_tag="ACIUS0_001073"
     CDS             complement(4356..6677)
                     /locus_tag="ACIUS0_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGB0218921.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACIUS0_001074"
     CDS             complement(6774..7250)
                     /gene="greB"
                     /locus_tag="ACIUS0_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGB0218922.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7478..8197
                     /gene="ompR"
                     /locus_tag="ACIUS0_001075"
     CDS             7478..8197
                     /gene="ompR"
                     /locus_tag="ACIUS0_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGB0218923.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8194..9546
                     /gene="envZ"
                     /locus_tag="ACIUS0_001076"
     CDS             8194..9546
                     /gene="envZ"
                     /locus_tag="ACIUS0_001076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGB0218924.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACIUS0_001077"
     CDS             complement(9622..11244)
                     /gene="pckA"
                     /locus_tag="ACIUS0_001077"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGB0218925.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11623..13374
                     /locus_tag="ACIUS0_001078"
     CDS             11623..13374
                     /locus_tag="ACIUS0_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGB0218926.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13518..14387)
                     /gene="hslO"
                     /locus_tag="ACIUS0_001079"
     CDS             complement(13518..14387)
                     /gene="hslO"
                     /locus_tag="ACIUS0_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGB0218927.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14412..14813)
                     /gene="hslR"
                     /locus_tag="ACIUS0_001080"
     CDS             complement(14412..14813)
                     /gene="hslR"
                     /locus_tag="ACIUS0_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGB0218928.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14824..15492)
                     /gene="yrfG"
                     /locus_tag="ACIUS0_001081"
     CDS             complement(14824..15492)
                     /gene="yrfG"
                     /locus_tag="ACIUS0_001081"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGB0218929.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15557..17692)
                     /gene="igaA"
                     /locus_tag="ACIUS0_001082"
     CDS             complement(15557..17692)
                     /gene="igaA"
                     /locus_tag="ACIUS0_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGB0218930.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            18012..18572
                     /gene="nudE"
                     /locus_tag="ACIUS0_001083"
     CDS             18012..18572
                     /gene="nudE"
                     /locus_tag="ACIUS0_001083"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGB0218931.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18737..21289)
                     /gene="mrcA"
                     /locus_tag="ACIUS0_001084"
     CDS             complement(18737..21289)
                     /gene="mrcA"
                     /locus_tag="ACIUS0_001084"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGB0218932.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21409..22188
                     /gene="hofM"
                     /locus_tag="ACIUS0_001085"
     CDS             21409..22188
                     /gene="hofM"
                     /locus_tag="ACIUS0_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGB0218933.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22188..22727
                     /gene="hofN"
                     /locus_tag="ACIUS0_001086"
     CDS             22188..22727
                     /gene="hofN"
                     /locus_tag="ACIUS0_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGB0218934.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22711..23151
                     /gene="hofO"
                     /locus_tag="ACIUS0_001087"
     CDS             22711..23151
                     /gene="hofO"
                     /locus_tag="ACIUS0_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGB0218935.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23141..23547
                     /gene="hofP"
                     /locus_tag="ACIUS0_001088"
                     /pseudo
     CDS             23141..23547
                     /gene="hofP"
                     /locus_tag="ACIUS0_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23459..24697
                     /gene="hofQ"
                     /locus_tag="ACIUS0_001089"
     CDS             23459..24697
                     /gene="hofQ"
                     /locus_tag="ACIUS0_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGB0218936.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25098..25619
                     /gene="aroK"
                     /locus_tag="ACIUS0_001090"
     CDS             25098..25619
                     /gene="aroK"
                     /locus_tag="ACIUS0_001090"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGB0218937.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25676..26764
                     /gene="aroB"
                     /locus_tag="ACIUS0_001091"
     CDS             25676..26764
                     /gene="aroB"
                     /locus_tag="ACIUS0_001091"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGB0218938.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26856..28148
                     /gene="damX"
                     /locus_tag="ACIUS0_001092"
     CDS             26856..28148
                     /gene="damX"
                     /locus_tag="ACIUS0_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGB0218939.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28255..29091
                     /gene="dam"
                     /locus_tag="ACIUS0_001093"
     CDS             28255..29091
                     /gene="dam"
                     /locus_tag="ACIUS0_001093"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGB0218940.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29109..29786
                     /gene="rpe"
                     /locus_tag="ACIUS0_001094"
     CDS             29109..29786
                     /gene="rpe"
                     /locus_tag="ACIUS0_001094"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGB0218941.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29779..30537
                     /gene="gph"
                     /locus_tag="ACIUS0_001095"
     CDS             29779..30537
                     /gene="gph"
                     /locus_tag="ACIUS0_001095"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGB0218942.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30530..31534
                     /gene="trpS"
                     /locus_tag="ACIUS0_001096"
     CDS             30530..31534
                     /gene="trpS"
                     /locus_tag="ACIUS0_001096"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGB0218943.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31804..32709
                     /gene="yhfZ"
                     /locus_tag="ACIUS0_001097"
     CDS             31804..32709
                     /gene="yhfZ"
                     /locus_tag="ACIUS0_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGB0218944.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32726..33088
                     /locus_tag="ACIUS0_001098"
     CDS             32726..33088
                     /locus_tag="ACIUS0_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGB0218945.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33172..34335
                     /locus_tag="ACIUS0_001099"
     CDS             33172..34335
                     /locus_tag="ACIUS0_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGB0218946.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34336..35562
                     /locus_tag="ACIUS0_001100"
     CDS             34336..35562
                     /locus_tag="ACIUS0_001100"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGB0218947.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35559..36437
                     /locus_tag="ACIUS0_001101"
     CDS             35559..36437
                     /locus_tag="ACIUS0_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGB0218948.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36448..36801
                     /locus_tag="ACIUS0_001102"
     CDS             36448..36801
                     /locus_tag="ACIUS0_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGB0218949.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36813..38117
                     /locus_tag="ACIUS0_001103"
     CDS             36813..38117
                     /locus_tag="ACIUS0_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGB0218950.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38129..39214
                     /locus_tag="ACIUS0_001104"
     CDS             38129..39214
                     /locus_tag="ACIUS0_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGB0218951.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39374..40105)
                     /gene="frlR"
                     /locus_tag="ACIUS0_001105"
                     /gene_synonym="yhfR"
     CDS             complement(39374..40105)
                     /gene="frlR"
                     /locus_tag="ACIUS0_001105"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGB0218952.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40205..40990)
                     /gene="frlD"
                     /locus_tag="ACIUS0_001106"
     CDS             complement(40205..40990)
                     /gene="frlD"
                     /locus_tag="ACIUS0_001106"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGB0218953.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40987..41817)
                     /gene="frlC"
                     /locus_tag="ACIUS0_001107"
     CDS             complement(40987..41817)
                     /gene="frlC"
                     /locus_tag="ACIUS0_001107"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGB0218954.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41867..42889)
                     /gene="frlB"
                     /locus_tag="ACIUS0_001108"
     CDS             complement(41867..42889)
                     /gene="frlB"
                     /locus_tag="ACIUS0_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGB0218955.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42910..44247)
                     /gene="frlA"
                     /locus_tag="ACIUS0_001109"
                     /pseudo
     CDS             complement(42910..44247)
                     /gene="frlA"
                     /locus_tag="ACIUS0_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44542..>44702)
                     /locus_tag="ACIUS0_001110"
     CDS             complement(44542..>44702)
                     /locus_tag="ACIUS0_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGB0218956.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
BASE COUNT        10417 a        11343 c        12232 g        10710 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tactcgccgc agccaactct
       61 tcttctgaca gcccggtaaa tcgcatgatg tctgcaagag ggactccgga ttcaagcatt
      121 attttggcta tatgcagggc tttggattgt tccccctcct gccgcaatct ttccgcaata
      181 gtcattaaac tctccttgtg tttcggtgaa cgttcggcaa cgccgtcgat aaaatcgtta
      241 aaacgtacag cgtcgccagt ttgcagtatg taattaaaca gccctttgat ttgtctgtca
      301 ttagcgtatc cactacttaa taagcaggcc atttgctcta ccagccccat cagatcgcgt
      361 tgacgaatat gtttttgaat taactccagc agcgccatac gtcggtgctg catgatttca
      421 tcatcaggca tgacggtgac atcaatcagc ggaaatgcgg aggcataaag ctgccttgcc
      481 agtttgggat cggcgaaaca atccagccaa cacagcgaat agggataggg gctttcaata
      541 ccgtggtaaa acaacaatgg caccaccatc ggcaacgttt tgtatccagc atcaaggtga
      601 ttttgcattg cggcaatagc gtaacgcatc atgcgaaatg cgatcagttt gtttgaggtg
      661 ctttgatgtt caatcagaca atagatgtat cctggtcctt gttccgtttt caccgaccac
      721 agcacatcgg aatagctttc acgcagatcg tcatcaataa agctcctcga ctccagcttg
      781 agggttttca tatcacagag cgcgtgaatg ggtgccggta aatgaaacgc aagaaaatcg
      841 cgtgccgtct ccggttggcg taaaaagagt ttgaacagcg catcatgtgg ggtggaactc
      901 tgctttttac tcatggcgtt ccttcacctg ttgtttgaat gccgcgactc tacgcgccac
      961 tccacatctg tgcattagcg agttttcatc cctgcgagac gctttcagag gaacttacgt
     1021 tgcgctgcaa gaattgcatt tttatggaaa gccgcacgca aaaaagcccg gatgcaaacg
     1081 cataccgggc ttttcaaaac aggcgatgga gtatggttaa cgcagcgccc accactcgcg
     1141 cagacaggct ttgccttccg ggcagctttt acaactgcca gagaggcagc cgtcaggttc
     1201 ttcctgaatc cgcacggctt tgcccatgct ttccagctgt tgcagcatgg cgttaatcat
     1261 tggctgcgga gtgttcaatg tctggcttat ctgggtcgct tccatacggc cccgtaacgc
     1321 cagcaaatca cgtacctgaa taagtgaagc cattaatggc aatcaccggt ggtgctggct
     1381 gcgcagcaac tgcttaccga cttgcgggtt gccagcagtt cgatatccac ccggctacgc
     1441 gcgcggcgca gcagaccgat agctacgatg ttaaacagga ttaccgccag aatgcacacc
     1501 aggctgtaag ttgggtgctg actgtagctg gcgacctgat agaacaatgt tgccagtgag
     1561 taagcgatat tcagccccca caggatggag aagcccatcc agccacggct tgattcacgg
     1621 gcgatggctc ccatcaccga gatacatggt acatacagca ggacgaaaat caggtagctg
     1681 tatgcggcag ctgcgctacc gaatttctga tccatcacgc ccatcgcccc ggtacccatt
     1741 tcaccgttgc ctttgctagc ttcaatgggg ttcatcagta cgctaaggct gaaggtgtct
     1801 ttcaggctct gccaggtttc atctaccgca ctgaacagct cttcaccgag gttgaattcc
     1861 gccggattga actcttcgtc ctgaatattt tctgcggtgt agagggtgtt gagcgtaccc
     1921 accaccactt cttttgccat tgcgcccgta aacaggccaa ccgttgcctg ccagttatct
     1981 tcatgtacgc caatcggctt gaagacaggg gttatcaccc ggctgacgga agccagcgcc
     2041 gagtcgttga tgttatcgac gattttcccg ctcaacgaga agctgttgaa agcgctcagg
     2101 aaaatgctga cgatgataat cactttacca gcacgcagaa cgaagccctt cagacgctgc
     2161 caggtctgga taatcaggct tttaacgtgt ggtacatggt agaccggcag ctccatgaca
     2221 aacggcgtcg cttcaccgcg catgatggtg tacttgagca tcaggccagt cagcaccgcc
     2281 atcacgatac cgagcatata cagcgagaaa accgccaggg caccgttctg cccgaagaag
     2341 gcagccgcga atactgcgaa gatagccaga cgcgcgccgc aggacataaa cggtgccatc
     2401 atgatggtca tcagacgttc acgcggtgca tcaagcgtac gtgcgcccat taccgacggt
     2461 acgttacaac cgaaaccgac gatcagcggc acaaaggatt tccccggcaa gcccagcgcc
     2521 tgcatcaggc ggtccatcac aaacgctgca cgcgccatat agccggagtc ctcaaggaag
     2581 gagaggaaca ggtacatcat gccgatctgc ggcaccagtg gcagcacggt gttaataccg
     2641 ccacccaggc cctgggcgag gaagatagtc agccagtccg ggaagtggag cgtgtagcca
     2701 atccattgaa taccatgcac aaatagcgcc acggagccga cgttaaacag cggctgtaac
     2761 gccccgccga tgttgatagc cagcaggaac atcaggtaca tcacaaagag gaaaatcggc
     2821 agaccgagga aacggttgag cacgatttta tctaccgcag tggtgaaacg gctgggttct
     2881 gccgtcaggg tgttgcttac cacatcacag atggcagcaa tgcactggta acgcgcatcg
     2941 gcaatgtgca gcgccggatc atccatttca ttacgcagac gggcgagggt ggcatccaga
     3001 tgctgcgacg cttcaccggc gtaggcgcgg ctgtagatat cgccttccag catttgcagg
     3061 cccagccagc gacgttgttt cagcgggatg tcggaaggca tcacttttgc cagtgaatct
     3121 gcttcgttga gtagcggctg tgcgtaatgc accagttcca cattctcgtt agctttatag
     3181 cgatcaatcg ccagcttgag cgcttcaata ccgcgaccac gggttgaaac cagcgggatc
     3241 accgggcagc caagacgtgc cgatagagca tcaatttcaa tacgaatatt ttgcttctcg
     3301 gcaatgtcga gcatgttcag cgccacgatg cagggaatgc cgagttccag cagttgcagc
     3361 gtcaggtaca ggttacgctc aaggttagac gcatccacca cgttaatcag caggtcggcg
     3421 tcgccactca aaatgtagtg acaggcgatt tgctcatcga gcgaggtctg cgatgagatg
     3481 gtggtcagag aataggtgcc gggcaggtcc accagcgtga cctgatgatc ggtggtggag
     3541 aattgccctt ctttacgttc gacggtaacg ccagcccagt tacctacacg ctgacgtgcg
     3601 ccagtgagct ggttaaataa ggttgtcttg ccagaatttg gattaccaat taagccaatg
     3661 gttaattttt tcattgttgt tatcaccgta ttaacaggaa accgcttcca cttctaataa
     3721 ggccagatct tttttgcgta ataccaggct cacacgacgg gtttcgatat gaatggggtc
     3781 gccgagtgga gcgacgcgca ccacatcaaa agaggagcca ggtaacatgc ctagagaaag
     3841 cagtttttgg cgatatgccg ggctgatttc acgggaaaag ccagtgattt tccacgcagt
     3901 atctggagtg tattgcatag gtgcctactt gtttctcatt aactggataa ctacctcatg
     3961 gggcgtttga ccgcgacatg agaccagatg atttgccaac aaaggagaga ttaaataatc
     4021 aacaacacaa tgataatgag aatggtttct atcagcaata cttaaaatgt gtgcgaatgt
     4081 tggtttgaat aataatctga ctcaatgttg atgtggctca aggttatatc gcagcgaaaa
     4141 tataattcga atagtgagtt acccggactg gctaatgttt aattaaggtt tcagttgtta
     4201 atgaagtgat aaatattaac ggagtatatt gtgagtttgg ctgtggaata aaagtgccgg
     4261 agatattctc cggcactttt atgtcaggca aagcgttcgt tgccagatgc ggcgtgaacg
     4321 ccttatcgcg caggtttagg ggccagtccg ggcgtttaac gttttttacc catcgctgcc
     4381 gccagcgcat ccatcatcgc gctattaccg gcaggctgcg cttcacgacc gcgtggtttg
     4441 gctgccgggc ggttgttttg cgggcgttca ttaccgccgc cgcgacgaga gttggtttcg
     4501 ccaggctgct cgtccagacg catagtcagg gcgatacgtt tacgctgaag atccacttcc
     4561 agcactttca ccttcacaat gtcgcccgct ttcaccacgg tatgcggatc ttccacaaac
     4621 ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat gcacgccaat atcgacaaac
     4681 gcgccaaagt tggtgacgtt ggtcactgcg ccttcgagga tcatacccgg ttgcaggtcg
     4741 ttcattgtct cgacgccatc ggcaaactga gcggttttaa attccggacg cggatcgcga
     4801 cccggttttt ccagctcttt gatgatgtca gttactgtcg gcacaccgaa tttttcatca
     4861 gtaaagtcag acgctttcag gttacgcagt tcgctgctgt tacccatcag atctttcagt
     4921 gcctgctgtg ttgctgccag aatgcgttcc accaccggat aggcttccgg atgaacggta
     4981 gaggcgtcca gcgggttatc accgtggtta atgcgcagga agcccgcgca ctgctcgaag
     5041 gctttcggcc ccagacggct gactttcagc agttgctgac ggttctggaa ctggccgttc
     5101 tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg tcaggcccgc cacgcgggtt
     5161 aatagcggaa cagaagcggt gttgagatcg acgccaacgg cgtttacgca gtcttctact
     5221 actgcatcca gtttgcgggc cagttgcgtc tggctgacgt catgctgata ctgacctacg
     5281 ccgatagatt tcggatcgat tttcaccagc tccgccagcg gatcctgcaa acggcgggcg
     5341 atagataccg caccacgcag cgaaacgtcg agatccggga actcctgcgc tgccagctcg
     5401 gaagctgagt aaaccgacgc gccagcttcg ctgacgatca ctttctgtgc ggtcactttc
     5461 gggaactgct tctgcacgtc gagatagaaa cgttcagttt cgcgggaagc tgtaccgttg
     5521 ccgatcgcca ccagttcaac gttatgtttt tcacacaagg cagcaacggt catcgctgct
     5581 tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg ccaccagttt gccagtggca
     5641 tcgaccaccg ccacttttac cccggtacgc agacccggat cgaggcccat cgttgcacgc
     5701 agtccggcag gggccgccat cagcagatcg tgcaggttac gggcaaagac gttgattgct
     5761 tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg tttccagatg catcagcacc
     5821 ttgatgcgcc aggtccagct cactacgctt ttgcgccagc tatccgccgg ggcattgttc
     5881 aggcgcaggc caaggtgatc catgatgatt tgctcgcaat agctctcttt gggcggctca
     5941 tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc cttcgttacg cccacggaac
     6001 atcgccagcg cgcggtgaga aggcaccgtg gacaacggtt cgtgatgatc gaaatagtcg
     6061 cggaatttcg ccccttcctc ttctttaccg ctcaccaccg tagaaaccaa atgcgcgttc
     6121 ttccacagat aatcacgcac tttcgccagc agcgtggcat cttcggcaaa ccgttccatc
     6181 aggatatagc gcgcgccatc cagcgcggct ttggtatctg ccacgccttt atcggcatca
     6241 acatattgtg cagcggcgac ttctggcgtg tgtgacggat cgctccacag caggtcagcc
     6301 aacggctcaa gccctgcttc aatggcgatt tgcccgcggg tgcggcgttt aggtttgtag
     6361 ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg cgttgatggc cttcgccaga
     6421 tcatcggtga gtttgccttg ctcggaaatg gatttgagga tcgcctgacg tctctcttcc
     6481 agctcgcgca gatagctcag acgcgtttcc agattacgca gctgcgtgtc atccagaccg
     6541 ccggtgattt ccttacgata acgtgcgata aacggcacgg tattcccttc gtcaagcagg
     6601 cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt cacccgcaat aatgcggcag
     6661 aacgaatcat tcatcatggt ttgagttcat cttttcggat caaaaatcag gggatagtta
     6721 tacggactgg ctggcaaaaa tgccagccat cggcaggagg ttaagactct tccttacggt
     6781 ttcacgtact cgatagcatt aacataccag ttcgcttccc cggcaggggt attcaccacc
     6841 gccagatcgc cgacttcttt tttcagcaat gcgcgggcca tcggggaatc gatagagatg
     6901 taatctttac ggccaaaaat ttcatcgtag ccgacaatac ggaaacggtg agtcacgcca
     6961 tcgtcgtttt caatctccac ccacgcgcca aaaaagactt tgccttcctg ctgaggggag
     7021 taatcgacga ttttgagatt ttccaggcat ttagtgagat agcgcacgcg acggtcgatt
     7081 tcacgcagac gctttttatt atactgatag tcagcatttt cgctgcggtc gcccagactt
     7141 gcggcccagg tcaccttttt tgtgacctcc gggcgttctt cacgccagag ataattaagc
     7201 tcttgtttga gtttttcata cccttcccgg gtaaccaggg gcgttttcat ctcgttgatt
     7261 ccctttgtct gtttgataat gcgcacattg ggtataacgt gatcatatca acagaatcaa
     7321 taatgtttcg ccgaataaat tgtatactta agctgctgtt taatatgctt tgtaacaatt
     7381 taggctgaaa ttcataccag atttagctgg tgacgaacgt gagctttttt aagaatacac
     7441 gcttacaaat tgttgcgaac ctttgggagt acaaacaatg caagagaact acaagattct
     7501 ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa cgttatctca ccgaacaagg
     7561 cttccaggtt cgaagcgtcg ctaatgcaga acagatggat cgcctgctga ctcgtgaatc
     7621 tttccatctt atggtactgg atttaatgtt acctggtgaa gatggcttgt cgatttgccg
     7681 acgtcttcgt agtcagagca acccgatgcc gatcattatg gtgacggcga aaggggaaga
     7741 agtggaccgt atcgtaggcc tggagattgg cgctgacgac tacattccaa aaccgtttaa
     7801 cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt cgtcaggcga acgaactgcc
     7861 aggcgcaccg tcacaggaag aggcggtaat tgctttcggt aagttcaaac ttaacctcgg
     7921 tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc accagcggtg agtttgcggt
     7981 actgaaggca ctggtcagcc atccgcgtga gccgctctcc cgcgataagc tgatgaacct
     8041 tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc gacgtgcaga tttcgcgtct
     8101 gcgccgcatg gtagaagaag atccagcgca tccgcgttac attcagaccg tttggggcct
     8161 gggctacgtc tttgtaccgg acggctctaa agcatgaggc gattgcgctt ctcgccacga
     8221 agttcatttg cccgtacgtt attgctcatc gtcaccttgc tgttcgccag cctggtgacg
     8281 acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc tccagcagtt taataaagtc
     8341 ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc aactggagga cggcacgcag
     8401 ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg agctggggat ctctctctac
     8461 tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc aacactatga attcttaagc
     8521 catcagatgg cacagcaact gggcggcccg acggaagtgc gcgttgaggt caacaaaagt
     8581 tcgcctgtcg tctggctgaa aacctggctg tcgcccaata tctgggtacg cgtgccgctg
     8641 accgaaattc atcagggcga tttctctccg ctgttccgct atacgctggc gattatgcta
     8701 ttggcgatag gcggggcgtg gctgtttatt cgtatccaga accgaccgtt ggtcgatctc
     8761 gaacacgcag ccttgcaggt tggtaaaggg attattccgc cgccgctgcg tgaatatggc
     8821 gcttcggagg tgcgttccgt tacccgtgcc tttaaccata tggcggctgg tgttaagcaa
     8881 ctggcggatg accgcacgct gctgatggcg ggggtaagtc acgacttgcg cacgccgctg
     8941 acgcgtattc gcctggcgac tgagatgatg agcgagcagg atggctatct ggcagaatcg
     9001 atcaataaag atatcgaaga gtgcaacgcc atcattgagc agtttatcga ctacctgcgc
     9061 accgggcagg agatgccgat ggaaatggcg gatcttaatg cagtgctcgg tgaggtgatt
     9121 gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc tttaccccgg cagcattgaa
     9181 gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga atatggtggt caacgccgcc
     9241 cgttatggca atggctggat caaagtcagc agcggaacgg agccgaatcg cgcctggttc
     9301 caggtggaag atgacggtcc gggaattgcg ccggaacaac gtaagcacct gttccagccg
     9361 tttgtccgcg gcgacagtgc gcgcaccatt agcggcacgg gattagggct ggcaattgtg
     9421 cagcgtatcg tggataacca taacgggatg ctggagcttg gcaccagcga gcggggcggg
     9481 ctttccattc gcgcctggct gccagtgccg gtaacgcggg cgcagggcac gacaaaagaa
     9541 gggtaaataa acgggaggcg aaggtgcctc ccgttttgct ttctataaga tactggatag
     9601 atattctcca gcttcaaatc attacagttt cggaccagcc gctaccagcg cggcacccgc
     9661 aggggtgtcg gtgtatttat cgaagttgtc gataaacagt ttcgccaggg tttcggcttt
     9721 ttcctgccac tgttccggag aagcgtaggt gttacgcgga tcgagaatct tcgtgtctac
     9781 gcccggcagt tcggttggga tcgccaggtt aaacatcggc agagtgaagg tttctgcatt
     9841 atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg gtatctttaa tcgagatacg
     9901 tttgccagtg ccgttccagc cagtgttaac cagataagcc tgcgcgcccg ccgcctgcat
     9961 acgtttcacc agcacttctg cgtactgagt cgggtgcagc gacaggaacg ccgcgccgaa
    10021 gcaagcggag aaggttggcg tcggttcggt gatgccacgc tcagtaccgg ccagtttggc
    10081 ggtgaagcca gagaggaagt gatactgggt ttgatcggca gtcaggcgag aaaccggcgg
    10141 caacacgccg aaagcatcag cagtcaggaa gataacctta gtcgcgtggc ccgctttgga
    10201 aaccggctta acgatgttat cgatgtgata gattggataa gaaacgcggg tgttctcggt
    10261 ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc acggtgacgt tttccagcag
    10321 cgcatcacga cggatagcgt tgtagatttc aggttccgct tctttcgaca gcttgatagt
    10381 ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca tcgtcccagc cgtgttcgtc
    10441 atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg gttttaccgg tgccggaaag
    10501 gccgaagaac accgcaacat cgcctttctc gccaacgttg gcggagcagt gcatagaagc
    10561 gatacctttc agcggcagca ggtagttcat catcgagaac atccctttct tcatttcgcc
    10621 gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc aggttaaacg ccacgaagtt
    10681 ttcggagttc agaccctgtt ctttccactg cgggttagtg cacttcgcgc cgttcataac
    10741 gataaagtct ggtttgaaac ctgccagctc ttcatcgctc gggcgaataa acatgttttt
    10801 gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg acggaaagac gagtatccgg
    10861 gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg ccggaaagct gcctggtcac
    10921 caggcctttc agatgctgcc aggtttccgg agagagaggt ttgttgtcgt tcttaccttt
    10981 gcctttgtct gcccaccaga aagtatcgcg agtggtatcg tcacggacga tatacttatc
    11041 ttttggtgaa cgaccggtga agatcccggt atcgacggca acggcaccca gattagttaa
    11101 caccccgcgc tcataacctg tcaggctcgg atcgagctct tcctgataca gcaggtcgta
    11161 gcttgggttg taaacgatat catgtacgtc actgatacca taagcctcga gttcttgcgg
    11221 gggcaaacca ttgttaacgc gcatttcact gctccttagc caatatgtat tgcctgaata
    11281 gtaaagtctt tttgggggtg ttaaccgcga caaggctcat agatttacgt atctggagaa
    11341 attcatattt gacaaaaaac tccgtgattc ctgtcacgaa acggttgcta ttatcgcaga
    11401 aatagcattt taggtgtgga aaatgttctc aaaaggttaa atcttggttt atatggcggg
    11461 aatattgtga gtggaatcgc attcctgaat gaaattgatg caaattcaac cggtaaagta
    11521 tcgattcacc acgctaactt tttttgatta ttttctgaaa aaagcaaaat actactgccc
    11581 ggatgcgata attaacccac cttttgattc tggataacca acatggataa cgttgaactt
    11641 tcaccggcaa cacgctgggg gatgattgct accggattac ttcaggggat gattgctacc
    11701 ggattacttc aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac
    11761 agttggattg tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc
    11821 gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg
    11881 acagggatga gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa
    11941 aaggcgattt gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg
    12001 atacaacaaa gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca
    12061 gtatggcata acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg
    12121 gtgcttttac tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc
    12181 ttttttgcaa ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg
    12241 atcctcgcgc gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc
    12301 gccacggggt tgctgccgtt agtatcattg ctaaccctga tgtttatcat caccctgccg
    12361 tttacgggcc tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg
    12421 gcctttttgc aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc
    12481 tggacggggc cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg
    12541 ttcgtcgcca cctgggggtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc
    12601 ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt
    12661 atcgtctggc gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt
    12721 tcgttattgg tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt
    12781 attagcgtga acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta
    12841 actatctaca tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa
    12901 agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat
    12961 ggagagcaac atcttcagga acaggtatcg gaaaaagtat tcgccgaaaa tgtgttaatt
    13021 gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat
    13081 aacgtgatga cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt
    13141 gatggtcagg cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc
    13201 tttgactcag acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata
    13261 acgaaagaaa aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg
    13321 tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc
    13381 ttttatcgga tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct
    13441 ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga
    13501 catactcatt aatgaactta cggatctgcc ggagacgcgt tgttgcggat ttcagcaata
    13561 tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca
    13621 tcttccgcca ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa
    13681 cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct
    13741 tcttcgtgat acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt
    13801 ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgttgggt attttgcgca
    13861 ggcattacct gcaacaacat accgcctgca gccggtttgc cgtctacgtc gccggtgcga
    13921 ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttctag gcaggccgcc
    13981 agggtatcac cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg
    14041 gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg
    14101 ccctgcacgc gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga
    14161 ttcatcggac cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg
    14221 gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa
    14281 tcgtggttct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg
    14341 gcaaaatttt caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt
    14401 gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt
    14461 ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc
    14521 attttttcgc gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg
    14581 gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg
    14641 cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg
    14701 taatgcacct taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg
    14761 gcagcccata gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct
    14821 ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata
    14881 ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa
    14941 ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt
    15001 cagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa
    15061 tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac
    15121 cgccaggttg tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag
    15181 tgcctcaaga aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt
    15241 catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag
    15301 cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac
    15361 cccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc
    15421 gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat
    15481 gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac
    15541 ctgccgcgca aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca
    15601 ggcagctttc ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac
    15661 gccaggcctg tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg
    15721 tgctgagata gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg
    15781 taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca
    15841 tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa
    15901 gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga
    15961 atccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa
    16021 acggcgcggt ggaggttgcc accaggttat ccagcgattc agcactcacc gggcgtaata
    16081 acacattcac gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt
    16141 tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac
    16201 ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt
    16261 tttgaatcgc tgaacgtaat gaggcactga cgcgagattc atcttccggt ttagggtgca
    16321 gctggcgatt aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta
    16381 atggtaatga gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg
    16441 aattggtttt cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa
    16501 tacgcaatgt atcgcctacc cgcacgccag cgtcagccag ttgttttacg ctggtggctt
    16561 caatggtctg cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga
    16621 tccagaataa cagcataaac agcactagca gcgagcccgc agcgataatc gtactgcgca
    16681 gccagtgctg taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac
    16741 gcactacgtg gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa
    16801 tgtatggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga
    16861 tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat
    16921 gaatttctcg cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca
    16981 gcagcagtaa tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga
    17041 aaaagaacat caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac
    17101 tcagcgcata ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct
    17161 cttccccgcg aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt
    17221 cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg
    17281 tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt
    17341 ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt
    17401 aacgtgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag
    17461 agatcggtgc cgcgctagct tccgttgggc caggcacctg taatacctgc gtcaggctct
    17521 caagataatt ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg
    17581 cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg
    17641 caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc
    17701 catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg
    17761 ttggctatcg gcaagcgtgg agcttaactt gagcatcatt aagcacatca cagtaaccgc
    17821 tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct
    17881 attgactttt aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca
    17941 tgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca
    18001 tagatcccac gatgaacaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag
    18061 cccgttcccg actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc
    18121 gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg
    18181 acgatcacct gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt
    18241 tttcgaaagg attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa
    18301 aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac
    18361 cgtcttactt ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc
    18421 tggaaggcga tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg
    18481 atttgctgga agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc
    18541 gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg
    18601 cttatcaggc ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat
    18661 aacgcgttca cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc
    18721 cgaagcgcct ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc
    18781 ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc
    18841 tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg
    18901 gaggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc
    18961 atgcaggctg ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc
    19021 cggaagccgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg
    19081 tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg
    19141 ttttcccgcc gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc
    19201 agcctggttc accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag
    19261 tgttgatgac gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg
    19321 cctgctccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat
    19381 cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa
    19441 tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt
    19501 tttcaatttt gctgataaac cacgggtcca ctaggaagcc gccgttcgcc atgaccgcgt
    19561 agccgcgcgc cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt
    19621 ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc
    19681 ccatcgcccg cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta
    19741 agcgaattgg accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac
    19801 ctgcatccca gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat
    19861 ccatcgccgc ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg
    19921 tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac
    19981 cattttgcgg attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc
    20041 atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt
    20101 tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt
    20161 ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg
    20221 cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg
    20281 taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc
    20341 gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct
    20401 gctgcacttt gcgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc
    20461 cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa
    20521 tctccggcgc gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct
    20581 gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac
    20641 gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg
    20701 ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac
    20761 cgacaccata ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt
    20821 ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca
    20881 tcgtgcgttc tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc
    20941 cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt
    21001 caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct
    21061 ccggtgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca
    21121 gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtca
    21181 ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca
    21241 gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc
    21301 catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccaga
    21361 cgcattgata tacccgtcag agtgacgggt gataaggaga tcatcacaat gacatttaag
    21421 atctggcaaa ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg
    21481 ggcgcaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc
    21541 aaagatgggc ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc
    21601 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    21661 tcatttccgc gcccgtcgat gtcccttggt gagcgggagc aaacggcctg gctgtcaggg
    21721 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    21781 tcactcagcc ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt
    21841 acgctggcag aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta
    21901 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    21961 cagtggctgt gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc
    22021 gcgaaggagc tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa
    22081 ggcggatttg acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt
    22141 ggagactttg ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aatccgccaa
    22201 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    22261 tgttcgttgc gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca
    22321 gcgccgaagc gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct
    22381 tacagataac gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac
    22441 ggcagcgcca gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc
    22501 ttttacccga gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca
    22561 aggggctgac aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg
    22621 cgtcttttca tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat
    22681 ttgagtatca gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc
    22741 acatcacccc gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc
    22801 atttttctat catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt
    22861 catcaccagc agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa
    22921 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg
    22981 catccatccg cgcagggagg tgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    23041 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    23101 ggtgatgatc ttttgttcac gctacaactg gagatgccgc atgagggtta aacgctggtt
    23161 gttggcaggt attgcattgt gccttttaac cggtatgcgt gaccctttta aatcgccgga
    23221 agatctatat gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga
    23281 ggcgagcgca tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa
    23341 aacgatgtgc tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg
    23401 ggtaccggga caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat
    23461 gaagcaatgg atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc
    23521 gcaaaaagtg acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc
    23581 tgaacaggag aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca
    23641 tctaacagat gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat
    23701 aacgcggcag gaaggcaaca ttctctcagt gcattccatt gcctggcaga atgacaatat
    23761 cgcccgccag gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag
    23821 tataaccctg caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag
    23881 tgccaaaggg agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa
    23941 aacggcgtta agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt
    24001 tgagctgtcg gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa
    24061 atggacgctg gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag
    24121 cgacctctcc gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg
    24181 acgcttgctg gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc
    24241 cagtccgcgt ctgctggcct cacatcttca gcctgccagc attaaacagg agagcgaaat
    24301 tccatatcag gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt
    24361 cctggggatg gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca
    24421 catcagccag aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat
    24481 tgataagcag gagatcgaaa cgcaggtcga ggtaaaaagc ggagaaacgt tggcgctggg
    24541 cggcattttt acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat
    24601 tccctggttc gggcaattat ttcgtcatga cggaaaagaa gatgaacgac gcgagttagt
    24661 ggtgtttatc acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt
    24721 tttatgctga acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac
    24781 cgatttgaga gttggtgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg
    24841 cggcattttg ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg
    24901 tagcaaggca gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt
    24961 gccaaacccg ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg
    25021 tttcagttca tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc
    25081 ttagtagtac cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg
    25141 ccggaaaaag cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt
    25201 ccgatcaaga gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag
    25261 gcgaagaagg cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg
    25321 gtattgtgct ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt
    25381 ccgctcgtgg cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc
    25441 agcgtgataa aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag
    25501 cgttggccaa tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg
    25561 atgatcaaag cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat
    25621 tctggcttta tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga
    25681 gaggattgtc gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt
    25741 taatgaacca gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccag
    25801 cgaaaccctg gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt
    25861 taacgtcgat agcgttatcc tccctgacgg cgagcagtat aaaagcctag ctgtactcga
    25921 taccgtcttt acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct
    25981 tggcggcggc gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt
    26041 tcgtttcatt caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa
    26101 aactgcggtc aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc
    26161 agtggtggtg gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct
    26221 ggcagaagtc atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga
    26281 gaatctggat gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg
    26341 ttgtgaactg aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt
    26401 actgaatctg ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg
    26461 gttacatggt gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct
    26521 cgggcagttt agttctgccg aaacgcagcg tattataacc ctgctcacgc gggctgggtt
    26581 accggtcaat gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga
    26641 caagaaagtc cttgcgggag agatgcgctt aattcttccg ttggcaattg gtaagagtga
    26701 agttcgcagc ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc
    26761 gtaacaacaa gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca
    26821 acgtggtaag cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg
    26881 agctgaaacc cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt
    26941 ctgagcgtac tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca
    27001 ctgacgatcg ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag
    27061 aagaaattga cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc
    27121 gtaagcgcaa aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg
    27181 gcattctggt tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga
    27241 ccacttccag cgatcaaacc gcgtctggcg agaagagtat tgatcttgct ggcaatgcga
    27301 ccgatcaggc gaatggcgtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc
    27361 aggatgtttc tctgccaccg atctcttcta cgccgactca agggcaaacc ccggtggcaa
    27421 cggatggtca acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg acccagccac
    27481 aaaatcagca acagctgaac aatgtggcgg tcaattccac gttgccgact gaacccgcaa
    27541 cggttgcgcc tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg
    27601 aacgtccgtc cactacgcgc ccagttcgtc agcaggcggt gattgaaccg aaaaaaccgc
    27661 aagcaaccgt gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaacctg
    27721 aaccagctgc ccctgtggcg agcacgaagg caccggctgc gacttctacg ccagcaccaa
    27781 aagagacggc gactacggct ccagtacaga cggcatcccc ggcgcaaacc acggcaacac
    27841 cagccgctgg agggaagacc gcaggtaatg ttggttcgtt gaaatcggca ccgtccagcc
    27901 attacactct gcagctgagc agttcctcta actacgacaa cctgaacggt tgggcgaaga
    27961 aagagaatct gaaaaactac gttgtctatg aaacgacgcg taatggtcag ccgtggtatg
    28021 tcctggtttc tggcgtgtac gcttcgaaag aagaggcgaa aaaagcggta tctacattgc
    28081 cagcagatgt tcaggccaaa aacccgtggg cgaaaccgct gcgtcaggta caggccgatc
    28141 tgaagtaatc aaggttatct cccgcaatgg tttatcgttg cgggagctgc ctgaagcgct
    28201 ggatgctgtc ggagctttct ccacagccgg agaaggtgta attagttaat cggcatgaag
    28261 aaaaatcgcg cttttttgaa gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa
    28321 cggcatttgc ccaagggcga atgtctggtt gagccttttg taggtgccgg gtcggtgttt
    28381 ctcaacaccg acttttctcg ttatatcctt gccgatatca atagcgacct gatcagtctc
    28441 tataacattg tgaagatgcg tactgatgag tacgtacagg ccgcacgcga gctgtttgtt
    28501 cccgaaacaa attgcgccga ggtttactat cagttccgcg aagagttcaa caaaagccag
    28561 gatccgttcc gtcgggcggt actgttttta tatttgaacc gctacggtta caacggcctg
    28621 tgtcgttaca atctgcgcgg tgagtttaac gtgccgtttg gccgctacaa aaaaccctat
    28681 ttcccggaag cagagttgta tcacttcgct gaaaaagcgc agaatgcctt tttctattgt
    28741 gagtcttacg ccgatagcat ggagcgcgca gatgatgcat ccgtcgtcta ttgcgatccg
    28801 ccttatgcac cgctgtctgc gaccgccaac tttacggcgt atcacacaaa cagttttacg
    28861 cttgaacaac aagcgcatct ggcggagatc gccgaaggtc tggttgagcg ccatattcca
    28921 gtgctgatct ccaatcacga tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg
    28981 catgtcgtca aagttcgacg cagtataagc agcaacggcg gcacacgtaa aaaggtggac
    29041 gaactgctgg ctttgtacaa accaggagtc gtttcacccg cgaaaaaata attctcaagg
    29101 agaagcggat gaaacagtat ttgattgccc cctcaattct gtcggctgat tttgcccgcc
    29161 tgggtgaaga taccgcaaaa gccctggcag ctggcgctga tgtcgtgcat tttgacgtca
    29221 tggataacca ctatgttccc aatctgacga ttgggccaat ggtgctgaaa tccttgcgta
    29281 actacggcat taccgcccct atcgacgtac atctgatggt gaaacccgtc gatcgcattg
    29341 tgcctgattt cgctgccgct ggtgccagca tcattacctt tcatccagaa gcctccgagc
    29401 atgttgaccg cacgctgcaa ctgattaaag aaaatggctg taaagcgggt ctggtattta
    29461 acccggcgac acctctgagc tatctggatt acgtgatgga taagctggat gtgatcctgc
    29521 tgatgtccgt caaccctggt ttcggcggtc agtctttcat tcctcaaaca ctggataaac
    29581 tgcgcgaagt acgtcgccgt atcgacgagt ctggctttga cattcgtctg gaagtggacg
    29641 gtggcgtgaa ggtgaacaac attggcgaaa tcgctgcggc gggcgcggat atgttcgtcg
    29701 ccggttcggc aatcttcgac cagccagact ataaaaaagt cattgatgaa atgcgcagtg
    29761 aactggcaaa ggtaagtcat gaataagttt gaagatattc gcggcgtcgc ttttgatctt
    29821 gatggtacgc tggtcgacag tgctcctggt cttgctgctg cggtagatat ggcgctgtat
    29881 gcgctggagt tgcccgtcgc aggtgaagaa cgcgttatta cctggattgg taacggcgca
    29941 gatgttctga tggagcgcgc attgacctgg gcgcgtcagg aacgtgcgac tctgcgtaaa
    30001 acaatgggga aaccgcccgt tgatgacgac attccggcag aagaacaggt acgtattctg
    30061 cgtaaactgt tcgatcgcta ctatggcgag gttgccgaag aggggacgtt tttgttcccg
    30121 cacgttgccg atacgttggg cgcgttgcag gctaaaggcc tgccgctagg cttggtcacc
    30181 aacaaaccga cgccgttcgt cgcgccgctg ctcgaagcct tagatattgc caaatacttt
    30241 agcgtggtta tcggcggcga tgatgtgcaa aacaaaaaac cgcatccgga cccgctgtta
    30301 ctggtggctg agcggatggg aattgcccca caacagatgc tgtttgtcgg cgactcacgg
    30361 aatgatattc aggcggcaaa agcggcaggt tgcccatcag ttggcttaac ctacggatac
    30421 aactacggcg aggctatcga tctcagccag cctgatgtaa tttatcagtc tataaatgac
    30481 cttctgcccg cattagggct tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc
    30541 catcgttttt agtggcgcac agccctcagg tgaattgacc attggtaact acatgggtgc
    30601 gctgcgtcag tgggtaaaca tgcaggatga ctaccattgc atttactgta tcgttgacca
    30661 acacgcgatc accgtgcgcc aggatgcaca gaagctgcgt aaagcgacgt tggatacgct
    30721 ggccttgtat ctggcttgtg gtatcgatcc tgagaaaagc accatttttg ttcagtccca
    30781 cgtaccagaa catgcgcagt taggctgggc actgaactgc tatacctact tcggcgaact
    30841 gagtcgcatg acgcagttta aagataaatc tgcgcgttat gccgagaaca tcaacgctgg
    30901 tctgtttggc tatccggtgc tgatggctgc ggacatcctg ctgtatcaaa ctaatctggt
    30961 accggtgggt gaagaccaga aacagcacct ggaactgagt cgcgatatcg cccagcgttt
    31021 caacgcgctg tatggcgaga tctttaaggt gccggagccg tttattccga aatctggcgc
    31081 gcgcgtaatg tcgctgctgg agccgaccaa gaagatgtcc aagtctgacg ataaccgcaa
    31141 taacgttatc ggcctgctgg aagatccgaa atcggtagtg aagaaaatca aacgcgcggt
    31201 cactgactcc gacgagccgc cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc
    31261 caacctgctg gatatccttt ctgcggtaac gggccagagc atcccagaac tggaaaaaca
    31321 gttcgaaggc aagatgtatg gtcatctgaa aggtgaagtg gctgatgccg tttccggtat
    31381 gctgactgaa ttgcaggaac gctatcaccg tttccgcaac gatgaagcct tcctgcaaca
    31441 ggtgatgaaa gacggcgcag aaaaagccag tgtacacgct tcccgtacgc tgaaagcggt
    31501 gtacgaagcg attggttttg tggcgaagcc gtaatatcaa aacgctcgga cggtcccctc
    31561 gcccctttgg ggagagggtt agggtgaggg gagcaggcaa gcgccagtgg gaattaccct
    31621 ttgctcgcat tgtcgaataa atcacttcgt catcactctg aaaccgggga aacccggttt
    31681 ttttatcctc ttttcgcaac aacccgaaaa aatgtgaagc gcctcgccgt ttccacatct
    31741 cgtcgtcgcc acttatcttc attttaatga agatataaat attcatttta ttgaaaatat
    31801 attatgcgtc gaacgtttat caaaaaagaa ggcgtcgtca tcacgacact ggcccgttat
    31861 ttgttgggtg aaaaatgcgg taatcgactg aaaaccatag atgagctggc aactgaatgc
    31921 cgttcatcgg ttggcctgac gcaggccgcg ttgaaaacgc tggaatcaag cggagcgata
    31981 cggattgaac gccgtgggcg caatggcagt tatcttgtcg agatggataa caaagcattg
    32041 ctgagtcatg tggatatcaa caacgtggta tgtgcgatgc ctttgcccta tacccgcttg
    32101 tacgaaggtt tagcgagcgg attgaaagcc cagtttgatg gcattccttt ttactatgcg
    32161 catatgcgcg gcgcggatat tcgcgtggag tgtttactta acggtgtcta tgacatggca
    32221 gtagtttccc gcctggcggc ggaaagttat ctcagccaaa acaatttgtg catcgccctc
    32281 gagcttggcc cgcatacgta tgttggtgaa catcaactga tatgccgcaa aggtgagtct
    32341 ggaaatgtta agcgagtagg tctcgatagt cgttcggcgg atcagaaaat catgaccgat
    32401 gtcttttttg gcgatagtga tgtggaacga gtcgatctct cttatcacga gagtttacaa
    32461 cgcattgtta aaggcgatgt cgatgcggtg atctggaacg tggtggcgga aaacgaactg
    32521 accatgctgg gattagaggc gacgccgcta acagacgatc cgcgattttt acaggccacc
    32581 gaagccgtgg tcctgacgcg agtcgatgat tacccaatgc aacaattgct gcgtgctgtt
    32641 gtagataaac acgccctgct tgcccatcaa caacgggtgg tgagtgggga acaggaaccc
    32701 agttattaaa cgaaaggcat ctgatatgga aaccagactc aacctgcttt gcgaggcagg
    32761 cgttattgat aaggacgtct gcaaaggcat gatgcaggtc gtcaacgtac tggaaaaaga
    32821 gtgccatctg ccggtgcgca gtgagcaagg aacgatggcg atgacacata tggcgagtgc
    32881 actgatgcgc agtcgccgtg gtgaagaaat agagccgctg gataacgagt tgctggcgga
    32941 actggcgcaa tccagccact ggcaagccgt tgtgcaattg catcaggagt tgttgaagga
    33001 attcgctctg gaagttaacc cgtgtgaaga aggctatttt ctggccaatc tttatggatt
    33061 atggatggct gctaacgaag aggtttgaag ctcccgacaa atccacgtcg cacaacctaa
    33121 atattcaaaa aaatgaatat ttaattcaag gaacagaaac gaggcaacaa gatgtttgta
    33181 gaagcattga aacgccagaa cccggcgctg atttccgccg cactaagttt gtggcagcag
    33241 ggcaaaatcg ccccggacag ctgggtgatc gacgtggatc agatactgga aaacggtaag
    33301 cggctcattg agacggcgcg gctttacggc attgaactgt atctgatgac caagcaattt
    33361 ggtcgtaatc cgtggctggc ggaaaaattg ctggcattag gctacagcgg cattgtggcg
    33421 gtggattaca aagaggcgcg agtcatgcgc cgcgctggtt tgccagtggc gcatcagggg
    33481 catctggtac aaatcccttg tcatcaggtt gctgacgccg ttgaacaggg caccgacatc
    33541 atcaccgtgt ttactctcga caaagcgcgg gaagtttctg cggcagcggt taaggccgga
    33601 cggggccagc ctgtgctgct caaagtttat agcgacgatg attttcttta tccggggcag
    33661 gagagcggtt ttgtacagca ttcgctgcac gaggtgattg ctgaaattaa gaaactgccg
    33721 gggctgcatt tagccggact aacccatttc ccttgcctgc tttgggatga ggctgctggg
    33781 aaagttttgc caacaccgaa tcttcacacg ctggtacaag cacgggatca actggcgaaa
    33841 tctggtattg cgattgagca actgaacgta ccttcagcga ccagctgcac ttcgctgcca
    33901 ttgctggcgg aatacggtgc gactcatgcc gaacccggtc atgcgctgac cggtactatt
    33961 ccggcaaacc agcagggcga tcagcctgaa cgtatcgcga tgctctggtt aagtgaaatc
    34021 tcccatcatt tccgtggcga cagctactgc tacggcggcg gttactaccg tcgtggtcat
    34081 gcgcaacatg cgctggtgtt tacgccagaa aatcaaaaga ttactgaaac caatctcaaa
    34141 actgtggatg acagcagtat cgactacacc ctgccgctgg caggcgagta tccggtgagc
    34201 agtgcagtgg tgctctgttt tcgcacgcag atttttgtca cccgtagcga tgtggtgctg
    34261 gtgtccggta ttcagcgcgg cgaaccagaa atagttggtc gttatgacag cctcggcaac
    34321 cctctggagg cataaatggc gcgatttgtg gtgttagtga ttgatagctt tggcgtaggg
    34381 gcaatgaaag atgtcacgct ggtgcgcccg caagatgccg gggcgaatac ctgtggtcac
    34441 atcctgagcc agttgccgca tttgcagcta ccaacgctgg agaagctggg gctaatcaac
    34501 acattgggtt atgcgccagg cgatatgcag ccgtcagatt ccgcaacctg gggcgtggca
    34561 gagctgcaac atgaaggtgg cgataccttt atggggcatc aggaaatttt aggcacgcgc
    34621 ccgttaccgc cgctgcggat gccttttcgc gatgtgattg accgtgttga gcaggcatta
    34681 gtttccgctg gctggcaggt ggagcgccgt ggcgatgatc tgcaatttct gtcggtcaat
    34741 caggcggttg cgattggcga taatctcgag gcggatttag gccaagtcta taacattacc
    34801 gccaatctct ctgtgatctc ttttgacgac gcaatcaaaa ttggtcgtat cgtgcgtgac
    34861 caggtacagg tcggtcgggt cattacattt ggcggcctgt taaccaacag tcagcgtatt
    34921 ctcgatgccg cagaaagcaa agaagggcgc tttattggta ttaatgcgcc gcgttctggc
    34981 gcttatgaca acggcttcca ggtcgtgcat atgggctacg gtgttgatga aaaagtgcag
    35041 gtgccgcaaa aactgtatga agcgggcgtg ccaaccgtgc tggtgggtaa ggtggcagat
    35101 atcgtcagca atccttatgg tgtgagctgg caaaacctgg tggatagcca gcggattatg
    35161 gatatcaccc tcgacgaatt taacacccat ccgacggcgt ttatttgcat caatatccag
    35221 gaaaccgacc tcgctggtca tgcagaagac gtcgcacgtt atgccgaacg tttgcaggtc
    35281 gttgaccgta accttgcccg gcttgttgag gcgatgcagc cagatgattg cctggtcgtg
    35341 atggcggatc acggtaacga tccgaccatt ggtcacagcc accatacccg tgaagtggtg
    35401 ccagtgctgg tttatcagca agggctggtc gccacgcagc tcggtgtgcg caccacgctt
    35461 tctgatgtgg gagctaccgt gtgtgaattt ttccgtgcgc caccgccaca aaatggtcgc
    35521 tcttttcttt cctccctccg gtttgcagga gacaccctat gagttttgat ccgacgggtt
    35581 acaccctggc ccatgagcat ctgcatattg atctctccgg ctttaaaaac aacgtggact
    35641 gccgccttga tcagtatgcg ttcatttgcc aggagatgaa cgacctgatg acccggggtg
    35701 tacgtaatgt gattgagatg accaaccgtt acatggggcg caatgcgcaa tttatgctcg
    35761 atgtaatgcg cgaaaccggg atcaacgtgg tggcctgtac tggttattac caggatgcgt
    35821 ttttcccgga acatgtggtg acccgcagcg tgcaggaact ggcgcaggag atggtcgatg
    35881 aaattgaaca gggtatcgat ggcacggagc tgaaagccgg gatcatcgcg gagatcggct
    35941 ccagcgaagg aaagattacg ccgctggaag agaaggtatt tattgccgct gcgctggcgc
    36001 ataaccagac cggacgcccg atctccacgc atacgtcgtt cagcaccatg gggctggaac
    36061 aactggctct gttgcaagcc cacggagttg atctttcgcg cgtcaccgtt ggtcactgcg
    36121 atctgaaaga caacctcgac aacattttga agatgatcga tctcggcgcg tacgtgcagt
    36181 tcgacaccat tggtaagaac agttactacc cggacgaaaa gcgcattgcg atgctccacg
    36241 cgctacgtga ccgtgggttg ctgaaccgcg tcatgctgtc gatggatatt acgcgccgct
    36301 cccatttaaa agccaatggt ggttatggct atgactattt attgaccacc tttattccgc
    36361 aattgcgcca gtcaggattc agtcaggccg atgtggatgt gatgttacgt gaaaatccct
    36421 ctcaattttt ccaataagga cagactcatg aaaaagattg gcgttgcagg cttacagcgt
    36481 gagcagatta aaaaaaccat tgaagcgacg gctcctggct gttttgaagt tttcattcac
    36541 aacgacatgg aagcggcaat gaaggtgaaa tccgggcaac tagattatta cattggcgcg
    36601 tgtaataccg gtgcgggcgc ggcattgtcg attgccatcg cggtgattgg ctataacaaa
    36661 agttgcacca ttgccaaacc aggcattaaa gcgaaagacg agcatatcgc caaaatgatc
    36721 gctgaaggga aagtggcgtt tggcctttcc gttgagcacg tcgaacacgc gattccgatg
    36781 ttgattaacc atctgaaata aaaggcacga ctatggatct gtatattcag attatcgtgg
    36841 tggcgtgcct gacgggtatg acatcgcttc tggagcatcg ctcggcggct gtttttcatg
    36901 acggcatcca cccgatcctg ccgcaactga ttgaaggcta tatgaaccgt cgcgaggcgg
    36961 ggagtatcgc ttttggtctg agcattggtt ttgtggcctc ggtggggatc tcttttaccc
    37021 tgaaaaccgg gctgctcaac gcatggttac tctttcttcc taccgatatc ctcggcgtac
    37081 tggcgataaa cagcctgatg gcgtttggtc ttggcgctat ctggggcgtg ttgatcctta
    37141 cttgcctgtt gccagttaac cagctgctga ccgcgctgcc ggtggatgta ttaggtagcc
    37201 tcggggaatt aagctcgccg gtggtttctg cttttgcact gttcccgctg gtggcgattt
    37261 tctaccagtt tggctggaag caaagtctgg tcgccgccgt ggttgttctg atgacccgtg
    37321 tggtagtcgt gcgctatttc ccacatctta accctgaatc catcgaaatc tttattggca
    37381 tggtgatgct gctggggatc gcgataactc acgacctgcg tcatcgtgat gaaaatgaca
    37441 tcgatgccag cgggctttcg gtgtttgaag agcgcacgtc gcggattatc aaaaacttac
    37501 cttatatcgc catcgtggga gcattgattg ccgccgttgc cagtatgaag atttttgctg
    37561 gtagcgaagt ttcgatcttc acactggaga aagcgtattc cgcaggcgtg acgccggaac
    37621 aatcgcaaac gctgattaat caggcggctc tggcagaatt tatgcgcgga ctggggtttg
    37681 tgccgttgat tgccaccacc gccttagcaa cgggtgtgta tgcagttgcg ggctttacct
    37741 ttgtttatgc ggtgggctat ctctcgccga atccgatggt tgcagcggta ttaggcgcag
    37801 tggttatttc ggcggaagtc ttgctgcttc gttcgatcgg caaatggctg ggacgctacc
    37861 cgtcggtgcg taatgcgtcg gataacatcc gtaacgccat gaatatgctg atggaagtgg
    37921 cgctgctggt cggttcgatt ttcgcagcaa ttaagatggc gggttatacc ggattctcta
    37981 tcgcggttgc catttacttc ctcaacgaat ccctgggccg tccggtacag aaaatggcgg
    38041 caccggtcgt ggcagtaatg atcaccggta ttctgctgaa tgttctttac tggcttggcc
    38101 tgttcgttcc ggcttaagga aggcacctat gaagacgttt cctttgcaaa gcctgactct
    38161 tgctgaagcg cagcaaaagc agtttgcgct ggtggacact atttgccgcc attttccagg
    38221 cagcgagttt cttgctggcg gtgatttagg gttaactcca agtctgaatc aaccgcgtat
    38281 tacccaacgg gtggagcagg tgctggctga tgcatttcac gcacaggccg cggcgctggt
    38341 gcagggcgcg gggactggcg cgattcgcgc cgggctggcg gctttgctca aaccggggca
    38401 gcgtcttctg gtgcatgacg cgcctgttta cccgacgaca cgggttatta ttgagcagat
    38461 ggggctgacg cttattactg ctgatttcaa tgacctgtcg gcactgaagc aggtcgtcga
    38521 cgagcaacaa cctgatgccg cgctggtgca gcatacgcgc cagcagccgc aggacagcta
    38581 tgtgctggca gatgtgctgg caacgttgcg cgcggcaggt gttccagcgt taaccgatga
    38641 taactatgcg gtgatgaagg tagcgcgaat cggctgtgaa tgcggcgcga atgtctcgac
    38701 attctcctgc ttcaagctgt ttgggccgga gggcgttggt gcagtggttg gcgatgccga
    38761 tgttattagt cggattcgcg ccacgcttta ctccggcggt agccagatcc agggctcaca
    38821 ggcgctggaa gtcttgcgtg gtctggtgtt cgcgccagtg atgcatgcgg tgcaggcggg
    38881 agtatccgaa cggttgctgg ctttgcttaa cggcggcgcg gtggcggaag tgaaaagcgc
    38941 cgtcattgcc aatgcgcagt cgaaggtgtt gattgtcgag tttcatcagc tgattgccgc
    39001 cagggtgctg gaagaagcgc aaaagcgtgg cgcgttgcct tacccggtcg gtgcagagtc
    39061 gaaatatgaa atcccgccgc tcttctatcg actttcagga acgtttcgcc aggcgaatcc
    39121 acaactcgaa cattgtgcga ttcgcattaa cccgaatcgc agcggtgaag agacggtgct
    39181 gcggattttg cgtgagagta ttgcgagtat ttaatgctgg atagcaccgt tcggcaatca
    39241 agctaaattc cgcactgaac ggtcccctcg cccctttggg gagagggtta gggtgagggg
    39301 aacgtgcggg atgtaaaatt cagtgcgcca gcatcgccgg atgcgatgct ggcgcatctt
    39361 atccggccta cggttaatgt cttttattat caatcgttaa ggtgatgcga ttggcgcggc
    39421 agaagagttc ggagacgtgc accggtttgt cattctgatc aaaggcgatt ttgctgatgc
    39481 gaaacaacgg ttcgcccagt tcgcatttca gccattttgc ctgcgggcgg gtggcggcaa
    39541 agatatcgat ggtctttttg tcgctgacca ctcgcgtgtc aaaacgctcc tgaaataact
    39601 gataggtgga gcttccttcg acgtaaatct catcaaagtc aggataacgc gacagcggga
    39661 tccatgaact atcaataaac agcggctctt tatcgagata catcacccgg cagagatgga
    39721 acacttcgct gtttccgggg atgttcagct tttcgcaaaa cggcgcggcg ctgacccgtt
    39781 cctgttcgat cactttctct ttcgtcgact tgccttgtga gacgccaaaa tcggtaaaac
    39841 cactgacagt aagcagggcg ttttcaactt tctggctttg tacaaaggtg ccttttccct
    39901 gccagcggat cagtacgccg tctgccacta agtcgctgat ggctttgcga atggtaatgc
    39961 ggctgacgtt atattgtgta caaagctcgt tttcggtagg gatctgttgc ccggcctggt
    40021 aaaccccctg cgcgatatca tccagcagcc gctggcggac ggtagcgtag aggagttgat
    40081 gagagtagcg gtccgtagct gacataatcg accctgaaat aaagaatgaa ttactcggat
    40141 tatagggtcg ttttccggca agtaacagca cgttggcgtg aagatgctca cgccaacgtt
    40201 atacctacca ggcaccgtgg tactgaatgg ttttcgccgc gcacgccgtt ccctgcgcca
    40261 tcgcctgcgg taatgtcatc cccgcagacc agccgcaaag gaatccggca atgaacgaat
    40321 ctccggcacc catggtgtcg ataaccgtca ccggttcagg agcctgacgc cagaactgcg
    40381 cgccatccca ggcaatgctg ccgttttcac ccagcgtgac aatcactgtt cctgcgccac
    40441 gggcaacaat cgctttcatc ttcagacgca gcgcttcgtc ttcttgcggt gcggaggcaa
    40501 aggcaaaatc gagatgcggc accagtgtct gccagagcgg gctgtcccac ttgtcggaga
    40561 agtcgaaagc ggtaagtttg cccgcagcgt gcagctgtgg gaatgcgtct tccgcatgtc
    40621 cccagattgc cgcgtgcaca atgtcatact gcgccagcca ggcgtaatcc tcttcactca
    40681 gggcaaagtc ggccatcacg ccttcggtgt agtcgccaaa aacgcgatca ttgtcgtgca
    40741 gttccacctg agtttgtgcg gtaacgccgt gtttcgtatg gacatggctg atatcgacgc
    40801 ccatgcgggc gagatcctgc ttcagctttg tgccgtagtc atcgtcaccc acccaggtaa
    40861 tgcatcccgg ctgtatgccg tagcgagtgc agtacaccgc cacattgacc gcattaccgc
    40921 cagaaaacgc tttattcagt tgcgggtaga tatcgacgca gttatcgccg attgtcgcca
    40981 gggttttcat ctctcatcct ccggcagcag cgcgcgaaag cgttccagcg cctggcgggc
    41041 atagagtctg ggctcgttca tatacatcgt caccagctcc accgtagagt aaccctcata
    41101 gccccgatca ataatatcgc gcatcagttc ccgcagcggc attttgcctt ctccaggaat
    41161 gtaatgcgtg tcgctggccc cgtcgctgtc gacaatatgc agatgacgta atttatcgcc
    41221 cagtttgtcg aaataactca tcaccggttc cgcctggaca tacggcgcgc aaatgtcgac
    41281 catgctgaac aggcgcggcg aaggcaccag cgccagcgca tgaagcacat cattagcatt
    41341 acacacgacg ttcgattcat acggcgttaa cggttcgagg atcagatcca tgccgatgtt
    41401 ttccgcgtac tcacacagtt cgcttaagtt ctctgccagc cgcccccaga taacgttagg
    41461 cggcgtgaga tagcccgcgt gggccgcaga aatcagcgta taacccgcgt tcatctcttt
    41521 tgccatatct atcgccagct tgatcatgtc caggctttca cgacgcatat gttcatcgcc
    41581 cagcatcatg ttatacggat agccgttggt ttctggcgtg taaccgataa tcggcatctg
    41641 atacgtctgc gccagcgcct tgatttgttt gatgccgccc gcttttaagt ccggcgcgaa
    41701 cgcgtgcggg cgaccgcccc aaatttcgat gccgtcgtaa cccagctcgc ttgcatcacg
    41761 aaatgcgtgt tcaataggca gccgctggtg gccgcaggta aacatacctg ttttcatcgg
    41821 gtaactcctt gcgtaaatcc ggcccgttgc cgggccggga gagggattaa tattccacca
    41881 gaccaccgta atagcggcgt tcatccgggt tgtgatcttt gtaaatagac aggtagtagc
    41941 agagccactc cattggcacg aacatcagga acggtgccag ccacgggtgc agcccttgcg
    42001 aaatttcggc gtaatcgatg acgatcacgt tgtcagtacg ctgtttaaca aagttaatgg
    42061 cgcgttcggt ggtgtggcga ctttcatcat tgccgagcag gaacaggaac ggaacgcccg
    42121 gttcgacaat ctccagcggg ccatggcgga actctccgct ctcaatcacg cagccgtgcg
    42181 tccaggtaaa ttccatcagc gttacaatgc cttctttgta acccagcgga cgcagcggac
    42241 ccgcagcaac ggtataaatc atcggccact ggctggccag ttcaccaagc tggcggcctt
    42301 tttcttccca ggtgcgtacc agatgaccga gcgcattcgg caactgcttg agatcgtttt
    42361 tgatcttgcc gatttccgcg ttcggcgcga ggcgggtgat catctccagc accacgctgt
    42421 agcagagcag caggtgaatt tcccagatac agtcggcctg ataatcaatg ctaaattccg
    42481 ccgccgaggt aatcgggctg tccgcgcgtt tggtgaacgc cgcagtgagt gctccgcagg
    42541 cccggcccag ctccagcgct ttgattacct cttccgtttt accgtagtca gaaacgccaa
    42601 tcaccgcgca acgatcgtcg aggcgatacg gggtgttatc gcagaactcc cagccggaaa
    42661 tggcgtagac ctgaagatcg gaaaagcgat ccgccagatg tttcgccgtt tgcgccgcgt
    42721 tgagtggcga gccgcaggca acgaaataaa tacgatcaat gtcgcgcttc accatctctt
    42781 ccacgatggc gtgcaccagc ggaacgtcat ggctgagaac tttttccact tcctgaacca
    42841 tattttcggt gaccagaaag tccacggtgc ttttatcaat atccaacatt ttattttctt
    42901 cagataacat tacgacaaag cgttgagctg gcggctacgc ttcgcccaga aggcgtaagc
    42961 aggcagacca gtagcaataa cgatggcggc gcagataagg ccgggaatcg gtgcccagac
    43021 aaaggttgag gcgaccagaa tgaggcttga cgcaatggcg agggtggtca tcagcccgaa
    43081 agccggagta cgccacagcg gtttgtaatc gtcgcgttta cgacaccaga tgatggagcc
    43141 gaaggtgagg gtatttttga aacacatcac cagggtgaaa taacccagca ggctggtgag
    43201 atcggaaacg aagatgaaga agatccccag cgccccttgc aggatgatgg agacatccgg
    43261 cgtgttgtat ttcggatgcg catggccgaa gcatttaaag aacaggttgt ctttcgccat
    43321 cgcgtattcc agccgcggct ggtacatcac gcagctggaa agcgaaccaa gaatgacgat
    43381 catcgccgtg atggcaacaa agagcccagc ggtgctgccg agtgcgggga tctaggtcag
    43441 ggcgtcggaa atcggcgttt cagaattggc gagtttgtcg aagggcatca ggccggaaat
    43501 caccagcgcc agcagggtgt agagcaccag aaccagcaga caggaaccaa tcagcgctcg
    43561 tggcatggtt tttccggggt ttttaatttc gccggtcata taacagatag aggccatgcc
    43621 ggtatacgac caactggtgg cagagatccc cgccagcagc gccataaagc tgcccgttgc
    43681 gccaatcgca gtggtggtaa gggcggcaaa attctccgct ttaaaccaga agatcccaag
    43741 gccaatgacg atagtgaacg ggataatttt ggcgatggta attagcgtct gaaacgctgc
    43801 gccgccttca acggagcgca ggtgtagcaa cataaaggcg ataattaatc cggcggcgat
    43861 aaatttaccg agcaacggat cgataggcgt taaaaagcca agattgctga caatcgccag
    43921 cgccataatc gacaatgacg gcgcatcgtt ggcccagaag ctggcccagc cggagaggaa
    43981 agccagcggt cggcttccgg catttttcag ataaacataa tctgcgccat tttccggata
    44041 agcggtggat agttccgcat agacgcacat ttgcgggatc acaattaacc cgccaatgac
    44101 aaacgcgagc accgtaagcc acggcgtgcc cgctgctttt gccacttcac ccacagatac
    44161 aaaaataccg gagccgacgg ttgtcccgac ggcgattgca agaacggccc aaaatccgag
    44221 cttgcgttgg agttcctggc ttcccatagc gatacctttt tcttgtccaa tacgttgtag
    44281 gggagagttt gcagccattt aaaaggtaac atgacaatgc atgatgaata taacaacata
    44341 ttatgttatg tgatgtggag gaagatcaca aaatttcgca caggatcgcg ctgtggctaa
    44401 tggatgtagt tataaaattg aatttaaagt gaaaatattt ttacgggcgg gggcaagaag
    44461 gacatagaaa caaatacgcc ctcggaaaat ccagagggcg tcgggcaatt aaaccggtgt
    44521 tagccgattt ctgtcagaga cttactgtgc agtaggaccg caaccgccaa tgattttaga
    44581 aatagaaatt gccgggtgca gcaggtagtc gtaagagcag ttcttatcac ggttttcaat
    44641 atgaccagta catgcagtta acgtagccag tactgcacct accagtgcaa ctttaataaa
    44701 tt
//
LOCUS       DBBBSB010000021        42378 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.021, whole genome shotgun sequence.
ACCESSION   DBBBSB010000021 DBBBSB010000000
VERSION     DBBBSB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42378
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.021"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..624
                     /locus_tag="ACIUS0_001111"
     CDS             <1..624
                     /locus_tag="ACIUS0_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218957.1"
                     /translation="LMDKYFTTLLKEISNQPLEDWDIIISNTGEKFSEVGLTKYAQHL
                     ASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDTASANRVFHQHVPAFNTDHMIQ
                     YFINAKEWNNARELLIKEEMLGMHNLILLENICMQKNAECMAHITFTLNKLTTQPAIT
                     KIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSASATQ"
     gene            complement(684..1175)
                     /gene="yajQ"
                     /locus_tag="ACIUS0_001112"
     CDS             complement(684..1175)
                     /gene="yajQ"
                     /locus_tag="ACIUS0_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGB0218958.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1343..2254
                     /gene="panE"
                     /locus_tag="ACIUS0_001113"
     CDS             1343..2254
                     /gene="panE"
                     /locus_tag="ACIUS0_001113"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGB0218959.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2217..2807
                     /gene="yajL"
                     /locus_tag="ACIUS0_001114"
     CDS             2217..2807
                     /gene="yajL"
                     /locus_tag="ACIUS0_001114"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGB0218960.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(2861..4309)
                     /gene="thiI"
                     /locus_tag="ACIUS0_001115"
     CDS             complement(2861..4309)
                     /gene="thiI"
                     /locus_tag="ACIUS0_001115"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGB0218961.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            4515..4757
                     /gene="xseB"
                     /locus_tag="ACIUS0_001116"
     CDS             4515..4757
                     /gene="xseB"
                     /locus_tag="ACIUS0_001116"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGB0218962.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            4757..5656
                     /gene="ispA"
                     /locus_tag="ACIUS0_001117"
     CDS             4757..5656
                     /gene="ispA"
                     /locus_tag="ACIUS0_001117"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGB0218963.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            5681..7543
                     /gene="dxs"
                     /locus_tag="ACIUS0_001118"
     CDS             5681..7543
                     /gene="dxs"
                     /locus_tag="ACIUS0_001118"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGB0218964.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            7598..8515
                     /gene="yajO"
                     /locus_tag="ACIUS0_001119"
     CDS             7598..8515
                     /gene="yajO"
                     /locus_tag="ACIUS0_001119"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGB0218965.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(8626..9144)
                     /gene="pgpA"
                     /locus_tag="ACIUS0_001120"
     CDS             complement(8626..9144)
                     /gene="pgpA"
                     /locus_tag="ACIUS0_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGB0218966.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9122..10099)
                     /gene="thiL"
                     /locus_tag="ACIUS0_001121"
     CDS             complement(9122..10099)
                     /gene="thiL"
                     /locus_tag="ACIUS0_001121"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGB0218967.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10177..10596)
                     /gene="nusB"
                     /locus_tag="ACIUS0_001122"
     CDS             complement(10177..10596)
                     /gene="nusB"
                     /locus_tag="ACIUS0_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGB0218968.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(10616..11086)
                     /gene="ribE"
                     /locus_tag="ACIUS0_001123"
     CDS             complement(10616..11086)
                     /gene="ribE"
                     /locus_tag="ACIUS0_001123"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGB0218969.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11175..12278)
                     /gene="ribD"
                     /locus_tag="ACIUS0_001124"
     CDS             complement(11175..12278)
                     /gene="ribD"
                     /locus_tag="ACIUS0_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGB0218970.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12282..12731)
                     /gene="nrdR"
                     /locus_tag="ACIUS0_001125"
     CDS             complement(12282..12731)
                     /gene="nrdR"
                     /locus_tag="ACIUS0_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGB0218971.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            12882..13421
                     /locus_tag="ACIUS0_001126"
     CDS             12882..13421
                     /locus_tag="ACIUS0_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGB0218972.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            13719..14603
                     /gene="tsx"
                     /locus_tag="ACIUS0_001127"
     CDS             13719..14603
                     /gene="tsx"
                     /locus_tag="ACIUS0_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGB0218973.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(14780..15127)
                     /gene="yajD"
                     /locus_tag="ACIUS0_001128"
     CDS             complement(14780..15127)
                     /gene="yajD"
                     /locus_tag="ACIUS0_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGB0218974.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15256..16227)
                     /gene="secF"
                     /locus_tag="ACIUS0_001129"
     CDS             complement(15256..16227)
                     /gene="secF"
                     /locus_tag="ACIUS0_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGB0218975.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16238..18085)
                     /gene="secD"
                     /locus_tag="ACIUS0_001130"
     CDS             complement(16238..18085)
                     /gene="secD"
                     /locus_tag="ACIUS0_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGB0218976.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18113..18445)
                     /gene="yajC"
                     /locus_tag="ACIUS0_001131"
     CDS             complement(18113..18445)
                     /gene="yajC"
                     /locus_tag="ACIUS0_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGB0218977.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(18468..19595)
                     /gene="tgt"
                     /locus_tag="ACIUS0_001132"
     CDS             complement(18468..19595)
                     /gene="tgt"
                     /locus_tag="ACIUS0_001132"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGB0218978.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(19650..20720)
                     /gene="queA"
                     /locus_tag="ACIUS0_001133"
     CDS             complement(19650..20720)
                     /gene="queA"
                     /locus_tag="ACIUS0_001133"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGB0218979.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     YLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            20813..21386
                     /gene="acpH"
                     /locus_tag="ACIUS0_001134"
                     /pseudo
     CDS             20813..21386
                     /gene="acpH"
                     /locus_tag="ACIUS0_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21391..23208)
                     /gene="malZ"
                     /locus_tag="ACIUS0_001135"
     CDS             complement(21391..23208)
                     /gene="malZ"
                     /locus_tag="ACIUS0_001135"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGB0218980.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23364..24737)
                     /gene="proY"
                     /locus_tag="ACIUS0_001136"
     CDS             complement(23364..24737)
                     /gene="proY"
                     /locus_tag="ACIUS0_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGB0218981.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(24813..26132)
                     /gene="brnQ"
                     /locus_tag="ACIUS0_001137"
     CDS             complement(24813..26132)
                     /gene="brnQ"
                     /locus_tag="ACIUS0_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGB0218982.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(26539..27834)
                     /gene="phoR"
                     /locus_tag="ACIUS0_001138"
     CDS             complement(26539..27834)
                     /gene="phoR"
                     /locus_tag="ACIUS0_001138"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGB0218983.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(27892..28581)
                     /gene="phoB"
                     /locus_tag="ACIUS0_001139"
     CDS             complement(27892..28581)
                     /gene="phoB"
                     /locus_tag="ACIUS0_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGB0218984.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            28771..29973
                     /gene="sbcD"
                     /locus_tag="ACIUS0_001140"
     CDS             28771..29973
                     /gene="sbcD"
                     /locus_tag="ACIUS0_001140"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGB0218985.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            29970..33113
                     /gene="sbcC"
                     /locus_tag="ACIUS0_001141"
     CDS             29970..33113
                     /gene="sbcC"
                     /locus_tag="ACIUS0_001141"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGB0218986.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            33239..34420
                     /gene="araJ"
                     /locus_tag="ACIUS0_001142"
     CDS             33239..34420
                     /gene="araJ"
                     /locus_tag="ACIUS0_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGB0218987.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(34563..35471)
                     /gene="mak"
                     /locus_tag="ACIUS0_001143"
     CDS             complement(34563..35471)
                     /gene="mak"
                     /locus_tag="ACIUS0_001143"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGB0218988.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            35596..36507
                     /gene="rdgC"
                     /locus_tag="ACIUS0_001144"
     CDS             35596..36507
                     /gene="rdgC"
                     /locus_tag="ACIUS0_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGB0218989.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(36585..37040)
                     /locus_tag="ACIUS0_001145"
     CDS             complement(36585..37040)
                     /locus_tag="ACIUS0_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0218990.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37037..37372)
                     /locus_tag="ACIUS0_001146"
                     /pseudo
     CDS             complement(37037..37372)
                     /locus_tag="ACIUS0_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(37527..37811)
                     /gene="ppnP"
                     /locus_tag="ACIUS0_001147"
     CDS             complement(37527..37811)
                     /gene="ppnP"
                     /locus_tag="ACIUS0_001147"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGB0218991.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(37883..38560)
                     /locus_tag="ACIUS0_001148"
     CDS             complement(37883..38560)
                     /locus_tag="ACIUS0_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGB0218992.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(38818..39009)
                     /gene="yaiA"
                     /locus_tag="ACIUS0_001149"
     CDS             complement(38818..39009)
                     /gene="yaiA"
                     /locus_tag="ACIUS0_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGB0218993.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39059..39583)
                     /gene="aroL"
                     /locus_tag="ACIUS0_001150"
     CDS             complement(39059..39583)
                     /gene="aroL"
                     /locus_tag="ACIUS0_001150"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGB0218994.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(39766..40224)
                     /locus_tag="ACIUS0_001151"
     CDS             complement(39766..40224)
                     /locus_tag="ACIUS0_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGB0218995.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40344..41153
                     /gene="proC"
                     /locus_tag="ACIUS0_001152"
     CDS             40344..41153
                     /gene="proC"
                     /locus_tag="ACIUS0_001152"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGB0218996.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41170..42285)
                     /gene="adrA"
                     /locus_tag="ACIUS0_001153"
                     /gene_synonym="dgcC"
     CDS             complement(41170..42285)
                     /gene="adrA"
                     /locus_tag="ACIUS0_001153"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGB0218997.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLCGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10665 a        11335 c        10579 g         9799 t
ORIGIN      
        1 ctaatggaca aatatttcac gactcttctt aaagagatca gtaatcaacc attagaagac
       61 tgggatataa taataagtaa tactggtgaa aaatttagtg aagtgggtct aaccaaatat
      121 gctcaacatc tcgcatcttt cgctgaagaa ttttatataa aagagcaaaa atcacattct
      181 gaaagtgaaa tgagaggact attttcatca cttgccgaat tatatagttt tggtaatgat
      241 actgccagcg caaaccgagt gttccatcag catgtccctg ccttcaatac agatcatatg
      301 attcaatatt ttataaatgc caaagaatgg aacaatgcca gggaattatt gataaaagag
      361 gaaatgttgg gtatgcataa tctgatattg ttggagaata tttgtatgca aaaaaatgca
      421 gaatgcatgg cacacattac ctttacgctt aataaattaa caacacaacc agcaataaca
      481 aaaatagata gcgtgggtaa tgagcaactt tatcagattg gcaaaatcta ccataagttg
      541 gaaattaaac ctgagccaga gcaacaggtt ttaattcagt cgctttatga ccgggcatca
      601 ggaagcgcct ctgcaactca ataaatgcat ggtcaaaagg gccaccacgg tggccctgac
      661 aatcagtagc tatatccaga aagttaatcg cggaagtttt tgaactggaa cggctgaccg
      721 agatcgccac cgcgcaccat cgccatgaca gcctgcaaat catcacgaga tttgccagtc
      781 acgcggatct catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttgatcatc
      841 ttgacgattt ttttctgagt ggcgctctca atgccctgtt tcagtttcgc ttccacaaac
      901 caggttttac cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg
      961 cgcttcagca gcttggcacg cagaatatcc agcaactgat tgacctggaa gtcggactcg
     1021 ctcaacactt tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg
     1081 aagtcaaaac gggactccac ttcgcgactc gcgttatcga ccgcgttacg tgcttcctga
     1141 agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat
     1201 gcgaagcata atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt
     1261 aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag
     1321 tggttgcggg tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca
     1381 attatggctt acagcacttt gtaaacaggg tcatgaagtt cagggctggc tgcgtgtacc
     1441 gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta acgaatcgct
     1501 gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga cgctgaaagc
     1561 atggcaggtt tccgatgccg tcaaaagcct cgcatccaca ctgcctgtaa ctacgccaat
     1621 actgttaatt cacaacggca tgggcaccat cgaagaattg caaaacattc agcagccatt
     1681 actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc
     1741 aaacggaatc acgcatatcg gtccggcacg gcaacaggat ggcgattaca gttatctggc
     1801 ggatattttg caaaccgtgt tgcctgacgt tgcctggcat aacaatattc gcgccgagct
     1861 gtggcgcaag ctggccgtca actgcgtgat taatccactg actgccatct ggaattgccc
     1921 gaacggtgaa ttacgtcatc atccgcaaga aattatgcaa atatgcgaag aagtcgcggc
     1981 agtgatcgaa cgcgaagggc atcatacttc agcggaagat ttgcgtgatt acgtgatgca
     2041 ggtgattgat gccacagcag aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg
     2101 ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat
     2161 tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga
     2221 gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacga
     2281 tcaacctctt ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgac agcgatggca
     2341 acctggcaat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgaag
     2401 tggctgatgg cgaatatgac gtgatcgtgc tgcctggtgg cattaaaggc acggagtgtt
     2461 ttcgcgatag cactctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg
     2521 tcgcggctat ttgcgccgcg ccagccaccg tgctggtgcc gcacgatatc tttccgattg
     2581 gcaatatgac cggcttcccg acgctgaaag acaaaattcc cgctgaacaa tggcaggaca
     2641 agcgcgtcgt ctgggatgca cgggtaaaat tgctgaccag ccaggggccg ggtacagcta
     2701 tcgactttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg
     2761 catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc
     2821 aaacgtcgca tctgaccaga tgcgacgtaa aaactacaaa ttacgggcga tataccttca
     2881 cattgttaaa gccttgctcg cgcagataga gcgcctgcag acggctcatc accccgcgct
     2941 cacaccacag cagccaggtt ttgttctggt cgagatcgcc aaatttggtg ctcagtttat
     3001 agaacggcag agaaaccaca tcaatccctt cgactttcag tggcttatct tcctgttcat
     3061 cgacagaacg gatatcgaga atcacgtcgt tcgggccgaa gccgttgacg gtttccactt
     3121 ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttcct
     3181 caaccacttt atcgagaata ctgaagtcga acttctcttc ttccgcttca atcttcgatt
     3241 taaccgcttt caccgtcggg cttttggaga tcacgccaca atattccggc atcgtgcgag
     3301 caaagtcttc ggtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaaa
     3361 tcagcggacg caggatcagc gtgtcagaga cgttatcaat caggcgcagg ttggtcagcg
     3421 tctgactgga cacctggccg agcgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt
     3481 cagccacttt agatgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt
     3541 cgtcgatttt ctcgagaatt tccccgacaa ccggttcgaa attaatggcg acaaaacgca
     3601 cgcggtggga gctgccaaag cggttccaca gataatgcgc cacctgacgc acgccaattt
     3661 catgcgctgc gccgccgagg ttaaagaagc agtaatgcac gcggcagccg cgacgcatca
     3721 acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct
     3781 gggtgccaat cgggaaacca ccaatacctt cgtagcgacc tttaatcagc aggaggcgat
     3841 cgtcttccac ttccagatga acagttacat ccgggttagt cagcttcacg cgcgcggatt
     3901 caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat
     3961 gtttgccacg gcgcttcacg cgtacgcaga aggttttgcc ttccagctga tcgcgatact
     4021 gaaccaacgc tttctcaaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga
     4081 gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagtcaga cgctggtttt
     4141 catctttcgc gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat
     4201 agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag
     4261 attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg
     4321 ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc
     4381 tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat
     4441 tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga
     4501 gtcagacatt cattatgccg aagaaaaatg aggcgcccgc cagctttgaa aaggcgctga
     4561 gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc
     4621 tgaacgagtt cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag
     4681 ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta
     4741 caccggacaa tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa
     4801 ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac
     4861 catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac
     4921 cggtcatatg tttggcgtta gcacaaacac gctggacgca cccgctgctg ccgtagagtg
     4981 tatccacgct tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg
     5041 tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga
     5101 cgctttacaa acgctggcgt tctcgattct aagcgatgcc aatatgccgg aagtgtcgga
     5161 tcgcgacaga atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg
     5221 cggtggtcag gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga
     5281 gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt
     5341 aagcgccgga gataaagggc gtcgtgctct gccagtactc gacaagtacg cagagagcat
     5401 cggccttgcc ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt
     5461 gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg
     5521 tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc aggcgctgaa
     5581 acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat
     5641 ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa
     5701 atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag
     5761 tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag
     5821 cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta
     5881 caacacccca tttgaccaat tgatttggga tgtgggacat caggcttatc cgcataaaat
     5941 tttgaccgga cgccgcgaca aaatcggcac catccgtcag aaaggcggtc tgcacccgtt
     6001 cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat
     6061 cagtgccgga attggtattg cggttgctgc cgagaaagaa ggcaaaaatc gccgcaccgt
     6121 ctgtgtgatt ggcgacggcg cgattaccgc tggcatggcg tttgaagcga tgaatcacgc
     6181 gggcgatatt cgtcctgata tgctggtggt cctcaacgac aatgaaatgt cgatttccga
     6241 aaatgttggc gcgcttaaca accatctggc tcagctgctt tccggtaagc tttactcttc
     6301 actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccg cccattaaag agctgctcaa
     6361 acgtaccgaa gaacatatta aaggcatggt ggtgcctggc acgttgtttg aagagctggg
     6421 atttaactac atcggcccgg tggacggtca cgatgtgctg gggcttatca ccacgctaaa
     6481 gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg
     6541 ttatgaaccg gcagaaaaag acccgatcac tttccacgcc gtgcctaaat ttgatccctc
     6601 cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga
     6661 ctggttgtgc gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg
     6721 tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgttact tcgacgtggc
     6781 tattgccgag caacacgcag tgacctttgc cgccggtctg gcgattggtg gatacaaacc
     6841 cattgtcgcg atctactcca ccttcctgca acgcgcctat gatcaggtgc tgcatgacgt
     6901 ggcaattcaa aagctaccgg tcctgttcgc cattgaccgc gcgggcattg ttggtgctga
     6961 cggtcaaact caccagggcg cttttgatct ctcttacctg cgctgcatac cagaaatggt
     7021 cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta
     7081 taacgatggc ccgtccgcgg tgcgctaccc gcgcggcaac gcggttggcg tggaactgac
     7141 gccgctggaa aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggt
     7201 gatccttaac tttggtacgc tgatgccaga aacggccaaa gtcgccgaat cgctgaacgc
     7261 tacgctggtc gatatgcgtt ttgtgaaacc acttgatgaa gcgttaattc tggaaatggc
     7321 cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggtag
     7381 cggagtgaac gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggcct
     7441 gccggacttc tttattccgc aaggaactca ggaagaaatg cgcgccgaac tcggcctcga
     7501 tgccgccggt atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg
     7561 ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc ccttaggaaa
     7621 aaccgacctt cgcgtttccc gactttgcct cggctgtatg acctttggcg agccagatcg
     7681 cggtaatcac gcatggacac tgccggaaga gagcagccgt cccataatta aacgcgcgct
     7741 ggaaggtggc ataaatttct ttgataccgc caatagctat tccgatggta gcagcgaaga
     7801 gatcgttggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtagtcg ttgccaccaa
     7861 agtgttccat cgcgttggtg atttaccgga aggattatcc cgtgcacaaa ttttgcgctc
     7921 tatcaacgac agcctgcgac gtctcggcat ggattatgtc gatatcctgc aaattcatcg
     7981 ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc
     8041 cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg ctcaggcgct
     8101 ggaactacaa aaacagcact gctgggcgca gtttgtcagt atgcaggatc actataatct
     8161 gatttatcgc gaagaagagc gcgagatgct gcctctgtgt tatcaggagg gcgtggcggt
     8221 gattccgtgg agcccgctgg cgcgggggcg actgacgcgt ccatggggag aaactaccgc
     8281 acgactggtg tctgatgagg tggggaaaaa tctctataaa gaaagcgatg aaaatgacgc
     8341 gcagatcgca gagcggttaa cgggcgtcag tgaagaactc ggtgcaacac gagcacaagt
     8401 tgcgctggcc tggttgttaa gtaaaccggg cattgccgca ccgattatcg gaacttcgcg
     8461 ggaagaacag cttgatgagc tgctgaacgc ggtggatatc actttgaagc cataacagat
     8521 tgccgagctg gaaacgccgt ataaaccgca tgcggtcgta ggatttaaat aagagtccag
     8581 gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg acagaatacc
     8641 cagcggccag tgatgaccga taaagtacag gatgcctgcg gaaatcaccc cggcgacaat
     8701 atcgtcgatc atgatcccca tgccgccatg tacattgcga tcaaaccagc ggatcggcca
     8761 cggcttccac atatccagaa tacggaaaat cacaaacccg gcggcaaccc actgccagtc
     8821 attggtcggc agcgccatga gcgtgatcca cataccaata aattcgtccc agacaatgct
     8881 gccgtgatcg tgcacgccca tatctttcgc cgtttgatga caaagataga cgccgataca
     8941 gatccccaac atcaccacca gcgagtagag ctgccagggt aaaaaggtca tcagatacca
     9001 gaaagggatc gccgccagcg atcccatcga gccaggaacg attgggctta atccacttcc
     9061 gaatccgaca gcaagcagat gccacggatt actcatcttc aggcgacttt tcgcgacatc
     9121 tttatggcgt gacaaaatgg tcatatcctt tccagtcaaa cgtgacaggt tcgccgtcac
     9181 gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta aacggtacgc
     9241 caaggtgtcc gagagcaaca tccagcgcgc cacggttcag ttccggcacc gtgaaacaca
     9301 actcgtaatc ttcaccgcca gagagcgccc agcgcagcgc ctgttccggt tcaacatggc
     9361 gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg
     9421 ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata gctgaatttg
     9481 ccagatcgcg cagtgcctgc ccctgtaaaa tacgcggcga tggacggaga tgacgtttga
     9541 tcaagtagtc agcatcttta gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc
     9601 cggcggcgct atcgcccggt gtaccggtca catagatcca gtcacccggt ttcgccccag
     9661 agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc attgataatg
     9721 gcccacgcgt ggtatcgccg ccaatgagtt gcatatcgta ataattgaga agatcaaaca
     9781 aactgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt aatgccagcg
     9841 tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc gccagtgctt
     9901 tataagccag atcagcagga tcgatatcag ggaggaaatg gttgcccgcc accagcgtat
     9961 cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa tcgtcgccga
    10021 tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg gcaatcaggg
    10081 agaactcgcc acatgccata cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc
    10141 atggaacagt cttccgtgaa tctaccggcc tggatattac tttttgttag ggcgaatcac
    10201 aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg cgccgaacga
    10261 tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcac tacgtttaga
    10321 cagttcgtac agcgcaatgc gcagtactgc tttttctacc tggcccagtt cttccagcag
    10381 gcgggacagg tagggcttca tcagtccgtc gaggtatgcg gtgttagtcg ccaccccggc
    10441 cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag ccaggaactg
    10501 gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg cctggacggc
    10561 acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac taatttcagg
    10621 ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct tctgcacctt
    10681 tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca gtggtcagaa
    10741 ccccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac
    10801 caccagcgac atattcaaag tgggcagtgc caccacgaat aaccgtaccc agcgcaatca
    10861 ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc tcataggcac
    10921 caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt ttcagtgcgt
    10981 caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg
    11041 cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc ttccttcggg
    11101 ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg ctgcttccct
    11161 ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc tacatgacgt
    11221 atctctttga atttaaattg gggggcgtcg gctaatttct caagccctgg cagcgagcat
    11281 aatccacggg cgtcgctgcc taatagttta ggtgcgatat agacaatcag ctcatcgact
    11341 aaacccgcct gtagcaatac gccagcgagc gttggccccg cttccaccca gatgctgtta
    11401 atctgctgtt tacccagttg catcatcagt acaaccagat ccagatgacc tttatgctct
    11461 ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg cgtacgcgcg
    11521 aaccaggttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg attttggcta
    11581 tcaatcacaa tacgtactgg ctggcggaga ttttgttgcg ggtagagcgc ctgagtttgt
    11641 tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac cgtggcgctg
    11701 ctggttaaaa tggcatgact ttgtgcgcgc tgtcgttgta cgtcgcgccg cgcctgaggc
    11761 gaagtgatcc actggctttc gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca
    11821 agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc tttattcaat
    11881 tgctcggctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg
    11941 taaagtccac gcccagcgac ctgcgggtta ggatcttgca tcgctgcgac cacgcgcgct
    12001 acgccagcgg cgatgagtgc gtcacagcac ggtggcgtac ggccatgatg gctacagggt
    12061 tcgagtgtca catacgccgt tgcgcctttg gctttttcac ccgccatacg caacgcgtgt
    12121 acttcggcat gtggttcacc cgctcgttgg tggtaacctt caccgacaat ttcgccatct
    12181 ttgacaatga cgcacccgac attcgggttg gggtgggtgg taaaacgtcc tcgttgcgcc
    12241 agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg
    12301 atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc aaaacggata
    12361 taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat catcttgctc
    12421 ggtacttcgc gctcaccggt ggcgcgcagc tgcgatttaa tatgattgat tgccatttca
    12481 acgtaatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct acgcaatttc
    12541 tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat aaccagctcc
    12601 gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg gcggcgtacg
    12661 gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc gaaacagaat
    12721 gggcaatgca tacggcgtcc tgaccaggtg gttaacggaa atctatttta ccctgaactg
    12781 acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttcg ctgcttttac
    12841 cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact aacgtttttc
    12901 gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcaa agtgaagtgc
    12961 gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc aatcaagaaa
    13021 cagtcaaaat cactcagcaa aacaggctga atgcaaaatc cagcagtggg gtttaccttc
    13081 tgcctggagc gaaaacaccg gcaagactgg aaagccagat cggtacttta cgtatgtcgc
    13141 tggtgaatat tacgcctgat acagatggca ccacccttac actacgtatt cagggtgagt
    13201 cgaatgatcc cttgcccgca ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa
    13261 tagacaactt tcaggaaatc aatgtgcaga atcaattgat taatgcccct gccagcgtcc
    13321 tcgcccccag cgatgttgat attccgttac aattaaaggg gatttctgtg gatcagttag
    13381 gctttgtacg tatccacgac attcaacctg ttatgcagta aacgtatttc gggacgattt
    13441 tgtgcgtccc gcaacatctt ccccgtcatt ttgttactct gcttacatca cctggattga
    13501 tagtaaaagt ttgcaacaag ggcgaaagtc agtacaatcc ccgcccgaat gtgtgtaaac
    13561 gtgaacgcaa tcgattacgt aaatgataga actgtgaaac gaaacatatt tttgtgagca
    13621 atgattttta taataggctc ctctgtatac gaaatattta gaaacgcaat ttgcgccttt
    13681 ttcactcccg caagggattt tcaaacagtg gcatacatat gaaaaaaaca ttactggcag
    13741 ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa aacgacaaac
    13801 cgcagtatct ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc tatcacaccc
    13861 gtttcggacc gcagatccgc aacgatacct accttgagta cgaagcattc gctaaaaaag
    13921 actggttcga cttctatggt tatgcggatg cgccggtatt cttcggcggt aactccgatg
    13981 ctaaaggtat ctggaaccac ggttctccgc tgtttatgga aatcgaacca cgtttctcca
    14041 tcgacaagct gaccaatact gaccttagct tcggtccgtt caaagagtgg tacttcgcga
    14101 acaactacat ttacgacatg ggtcgtaata aagatggtcg ccagagcacc tggtacatgg
    14161 gtctgggtac cgatatcgac actggcctgc cgatgagcct gtccatgaac gtctatgcga
    14221 aataccagtg gcagaactat ggcgcagcga acgaaaacga gtgggacggt taccgtttca
    14281 aaattaaata ctttgtgccg attaccgatc tgtggggcgg tcagctgagc tacatcggct
    14341 tcaccaactt cgactggggt tccgatttag gggatgacag cggtaacgca atcaacggta
    14401 ttaagacccg tactaataac tctatcgctt ccagccatat tctggctctg aactacgatc
    14461 actggcacta ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg aacgacgatg
    14521 cagaactgaa cttcggcaac ggcaacttca acgttcgctc taccggctgg ggtggttacc
    14581 tggtagtagg ttacaacttc tgattatgaa aatgccggga tttattcccg gcatttctga
    14641 ttgttaacaa cgtgtgcttt gttcatgccg gatgcggcgt aaacgctttg tccggcccac
    14701 aaaagcacgt aaattcaata tattgctgaa ataacgtagg cctgataagc gtagcgcatc
    14761 aggcaatttt acgttttaat cacttcttct tgttcatcat cgctttcagg tcagcgaatg
    14821 ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata acggtcgtac
    14881 catactgatc cgcttcggta tatttcgaat gctcatgatc gtggcaatag agacacaaca
    14941 attcccagtt actgccatct tccgggttat tggtatggtc gtgatcaatg tggtgaacgg
    15001 taagttcacg caggttagaa taaacaaact cgcgggaaca gcgaccgcag acccacggat
    15061 agatttttaa tgctttttcg cgatagccgc tttctaaccg tgcatagttt tttgggatga
    15121 tagccatgaa aagttacctg gatgaaatgt agggttacgc gcagtttccc aagatgcggg
    15181 gattttcgca atctccatac atcaaaaaaa atcccgatct tctgaccggg attttcaaca
    15241 tcaacgggaa cttgattaag gcagaattga cggctgatcc gccccttctt tttccacttt
    15301 ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcag atgctacgta
    15361 gatggaagat gcagtgccga tggaaacacc gataagcatg gtcagcgaga agccttccag
    15421 taccggacca ccgaagagat acagcatcag gataaccatc aaggtagtac cggatgtgat
    15481 caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg
    15541 acggatcttg cggaagtttt cacgaattcg gtccgatacc acgatactgt cgttaagcga
    15601 gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga tatggaataa
    15661 cgacaaaata cccagcgtaa tgataacgtc gtgcgtcagc gcaataacca cccctgccgc
    15721 cagtcgccac tcaaagcgga aacctacgta cacgaggata gacagcagcg ctgccatcaa
    15781 cgccatcgca ccggtttgcg caaggtcagc ccccacgctc ggaccgacga actcaatacg
    15841 cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaactt ggctgcccag
    15901 cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt cgtggctgct
    15961 accaaagttt tgcagcattg gctcttcaaa accggctttt tgcaatgcat cacgcattac
    16021 gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag
    16081 cccccagtta aagccgcgca cgcccataat aacgatagca gcgattaaca gcagaccaga
    16141 gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc cgtggtttag
    16201 ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc ttcttgacgc
    16261 gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg
    16321 acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg gtacccactg
    16381 cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata gaactgaatg
    16441 cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc aactcttctt
    16501 taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt aagacgatac
    16561 ccgcgatacc tggcatactc agtgttgcgc ctggcaacag cgacataata ccgacgatca
    16621 agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta tagaagatga
    16681 tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc tgctcaatgt
    16741 tctgcatacc cagggtcggg ccaattgtac gttcttcaac aatctgaatc ggcgcgatca
    16801 acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg ttgatgccgg
    16861 tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc acctcttcct
    16921 gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg tactccacaa
    16981 acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata atgttgccac
    17041 cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg
    17101 agtcggtgat atggtcacca gtcagaatta cgcgtttgta cagcacaacc ggctgacctt
    17161 cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat
    17221 caacattggt gtttaccaga cggaattcca gcgttgccgt cgcgcccaga atctctttcg
    17281 cacgcgcagt gtcctgaata ccgggcagtt caacaacgat acggtcagca ccctgacgct
    17341 gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata ttaatgttct
    17401 gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc gcacgcagct
    17461 ggttgctgcc ctggctgcta atcaccaggt ccggatggcg cttgctcaga tacgcaatgg
    17521 cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagttg ttttctttac
    17581 gaacagtggt atacgggatg cccttttcgc gcaggtcact gcgtaggcta tcgatatttt
    17641 gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag tgaacgccgc
    17701 cacgcaggtc aaggccgagc ttcatcggct cagcatgaat agctgccagc cagcgcggcg
    17761 ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc attaatgctt
    17821 cacgagcgcg caactgggtg tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca
    17881 gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc tggatcagcg
    17941 tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa
    18001 acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc atgacgtact
    18061 tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt aattacagcg
    18121 ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata actacttcag
    18181 tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt acgcgaccaa
    18241 ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc agctttttgt
    18301 gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc agaccgaata
    18361 ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc gttgccgcta
    18421 ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta atcaacgttc
    18481 aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct ctctaattta
    18541 ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg aaggttatga
    18601 atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt cgagatgatg caagtaagcg
    18661 cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc agtatcgctc
    18721 ttatatttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg accatttcgg
    18781 gcgttgcggg ttggcattac gcagtcaaac atatcgatac cgcgacgtac gccttcaacc
    18841 aggtcttccg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg aatttgcgga
    18901 cacacatgct ccagaatacg gtgcatatct gctttcggct cacccacagc cagaccgccg
    18961 acagcgtagc catcaaagcc gatatctacc agacctttaa cagatatatc acgtaaatct
    19021 tcgtaaatac tgccctgaat tataccaaat aacgcatttt tgttaccaag actgtcaaaa
    19081 cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tagagcgttt tgcgtaatcc
    19141 cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga accaagatcg
    19201 tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta
    19261 cggaagtgaa cgccctgttc ggtgatttta cgaatatcac caaggctgaa gacctggaag
    19321 ccgccggagt cggtaagaat cggtcctttc cactgcataa aatcgtgcag atcgccgtgc
    19381 agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc gaggataatt
    19441 tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc gtaggtgcca
    19501 acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg gccacggcgt
    19561 gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg tcagaaaaac
    19621 agtccaacgt ttaaaccagc gccgcggaat tactccccga cgcgctcatt aattgcctgc
    19681 ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc
    19741 gccgctttat aggcattcat ggtgtgttga taaccggcaa aggccgaaac cagcataatc
    19801 agcgtcgact ctggcaagtg gaagttcgtc accagcgcat cgaccacttt gtactggaag
    19861 cccggataga taaagatttg ggtatcgtca aagaacggtt cgataagatc gttttttgcc
    19921 gcctgtgccg cgctttccag cgaacgtact gaagtggtgc caaccgcaat cacccggtta
    19981 ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc agcgtattcc
    20041 gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca
    20101 acgtgcaacg tcacaaacgc catctccacg cctttggcgc gtaatttttc cagcaaaggc
    20161 tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc gctataaacg
    20221 gtttgataaa gttcgcggtc agcgtcttcg tccggacgat cgatatacgg cggcagcggc
    20281 atatggccga tgctgttgag gatatccagt accgagcgtt catcattaaa ttcgacttca
    20341 aacagtgcgc cgtggcgcgc ggtcattgtt gcgttgatac tttcgtcatc gcccagcaac
    20401 agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg
    20461 tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc tggctttacg cccaaacaga
    20521 cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatcg
    20581 agtaaatagg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa
    20641 cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaag
    20701 gagaaatcgg taacgcgcat gacactgact cagactaaaa taagagtcgg gtagtctagt
    20761 gccggggcgc tctccctgca acctttaccc ccctttggat agaatataat cgatgaattt
    20821 tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttctggca atttactggc
    20881 tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca
    20941 tatgcatcga cgtattgact gacaatctgc cggaagtccg cgaagcacag gagtggtttc
    21001 gtagtgaaac gcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt
    21061 cccgccactg gtcgcagctg tcgccggact ttccgctaca ggaatttgtc tgttatgccc
    21121 gcgagcaagt gatgacgatt ttgccggact caccgccacg ttttatcaat ctgaacaatt
    21181 acttgtggtc agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt
    21241 taaacggcat ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc tggtacgatt
    21301 tagacgctca ttatgacgcc ctcgaaaccc gcttctggca gttttatccg cggatgatgg
    21361 cgcaggcgtc acacaaggcg ttataaaacg ttagttcatc cataccgtag ccgaaatggc
    21421 aggcaaagcc agaatcccgt cagtcagttg cccatgccct tctttgcatt gccattgcac
    21481 ggcattgaga aacggtgacg cgggtagcac cacttcactg gcctcgccac ggttgattgc
    21541 caccagtaca cgttgctgat tcagcacgcg gacaaatacc actacgttat cttccgcata
    21601 cagcacctga cagccgccac gacgcagcgc ctgacttttc ttacgcagca caatcattcg
    21661 ccggtacagc gcgaataacg ccgtatcctg cttttccacc tgccagggga acggtttacg
    21721 gcaaaacgga tcgtttttac catccagtcc cacttcatca ccgtaataaa tgcacggtac
    21781 accaggccag gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag
    21841 cagcgtttta aatcgcgcag tatcgtggct atcgagctga ttaaacatac gtaattgttg
    21901 ttgatgagaa agccctgcgc ggtaattatc catccaggcc atacaggttt gggcgtcaat
    21961 ttgctgcgga tcataagaga tgtcggtatt ggcaagaaat ccccacaacg ggaatgtgaa
    22021 gccacgatag ttcatggcag catcttccac atcggcctgt aaccattgcc gtgcatcgcc
    22081 aaaatgttcg ccgacaatat acgcttccgg ctgggtttct ttcgccgctt cggtgatccc
    22141 ggcaacgtgc tgcatattat tgcgcgcccc acccgcctcc cccagcatat gcaccacatc
    22201 cagccgccag ccgtccatat tccacggcgc tttcagccag tggcggacaa tactgtcttc
    22261 cccgcgataa atttcattca ccagactttc cgactgataa tccagcttcg tcaagctggc
    22321 atagccaagc cagtcgagcg ccgtgccatc atcactaaac gagtaccagt cgcgccaggg
    22381 cgattcagag ttgtgacaag ctccccccgt gccaagattg tgcctgtcaa accaggcatg
    22441 ggaatcgcca ctgtggttaa acacgccgtc cagcaccagc cgcattccca gctgctgcgt
    22501 attgtgtcgc aaacgcagca acgccccatc accgccaaac tgcggatcga catggcgata
    22561 atcctcggta tcgtatttat gtacgctggg agctttaaac accggattga gatacagcgc
    22621 tgtcacgcca agctttttca gatacggcag tttttcgctt atcccgtcca gatcgccgcc
    22681 atagaacgtt gatccgcccg cctgcgccgt gaccggttca tcccagtcac gcaagatgat
    22741 ctcttgtccg gctgcatgat ggtaatagac atgatcctgt tcagcttcac gaggaagact
    22801 acgcgcaaaa cgatcaggga agatctgata aaaaatctga tccgcagccc attgtgggcc
    22861 gatatcaggt acatcgacgg caaactgctc cagtcgtgcc ggcggcattc ggctgaagcc
    22921 ctgcggtgta aaccaacgct ggcgatcgtg ccacagcagt ttgaaactgt aacgccgccg
    22981 gggttgtccg ctggagagat caatcgccgc acgccatgcg gtgacgcctg gctgcggctg
    23041 actgcgctgc ttatgcattg gtactgacat ttcttcgtta tcgtgttctg tacgcagcat
    23101 aatgcgctgc ggtgggtctt cgcccgtcag ccacagtgta atgagcagtt gatctttgct
    23161 ttgtttaaca aatgggggca ccggcaggtg ccatgcattt aacatcataa ttcccctgtg
    23221 atgaaaatct gatcaccttg ccatagccta atgcaggatc actcatcatc tgaaggatat
    23281 ttgggggagg aggacggggg cggataactt tcggaatagc cggatcagga tttcctcctg
    23341 aaccggctta cagaacgcaa gtattattgg ttttcagcca gctgacgatc gtggcggcgt
    23401 ttaaacatcc agccaatcaa cagcacaaca atccacgcga aaccgacata cagcgagata
    23461 cgcgtatccg ggtgataacc aatcaacccg ataataaaga gcaggaaaat cagcccgccg
    23521 atggtcgttg ctaccccacc cggcacttta aatttcagcg ccttaacttc ttctggcggc
    23581 aaacggcgac ggaaggcgat ttgcgacagc aggatcataa tccacaccca taccgtggcg
    23641 aaggttgcca gcgaagcgat caccaggaag acgttttccg gcatgatgta gttcagatac
    23701 accgcaaaca gcagcgcggt agtcatcacc agcaccgtaa cccacggaat accgcgacgc
    23761 gacgttttgc tgaaaatttt cggtgcgctg ccctgctctg ccataccgtg gagcatacgg
    23821 cctacgccaa atacgtcact gttaattgcc gacagcgaag cggtcagcac aacaaagtta
    23881 agaatgctgg cggcaaaggt aatgcccata tgctggaacg tcagtacgaa cgggctaccg
    23941 gcagtgccaa cctgattcca cgggtagata gacataatga cgaacagcgt accgacgtag
    24001 aacaccagaa tacgcatcgg cacggagtta atcgcacgcg gaatcgattt ctcaggatct
    24061 ttcgcttcac cggcggtaat cccgataatt tcgatcccac cgtaagcaaa catcaccatt
    24121 tgcaacgaca ttaccatgcc aagccagccg ttactgaaga agccgccgtt gctccacagg
    24181 ttatgaatac cggtcggttg cccgccgttg ccaatccccc agatgatgat gccgaaaccg
    24241 gcgacaatca tgatgatgat ggtggcgact ttaaagaacg agaaccagaa ttccagctca
    24301 ccgaagacct tcacgctcat caggtttacg gcgcaaatga tcagcaccac gctcagtacc
    24361 caaatccagt gcggcaccgt cgggaacaag acacccatat agataccaaa agcggtcaca
    24421 tcggcgatgg cgacaataag gatttcaaaa cagtaggtcc atccggtaat gtaacctgcc
    24481 agcgggccaa ggttttcctg cgcataacgc gagaaagagc tggcggccgg gttatgtacc
    24541 gacatttccc ccagcgcacg cataatgata tacgccgcga taccaccgat aatataggcc
    24601 aacaacacgc tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa cagcccggtg
    24661 ccaattgctg aacccagtgc cataaagcgt atgtggcggg tacttagccc acgctttagc
    24721 ttattcttac tttccatcaa ttcctaaccc atcaacacaa taaaaaacca cggggcatcg
    24781 cagccccgtg gttaaaacaa atgttcagtg atttagtgag cgctggaggt cacctgacga
    24841 cctgccgcgc gatcccagat aatggccaga accaccatca ccactgttgg cattaaccac
    24901 gccagacctt gttcggccag cggtaaacgc tgcgcccagg acggtaagat atcgctgaat
    24961 gcagatgctt tgatcccgtc gagaatacca aaaagcaggc tgataaacat cggcggagca
    25021 atcacgcggg acgaattatg ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata
    25081 cacggcggat aaatagcggt cagcaccggt acggagatct ggatcagctg gcttaagccg
    25141 aggttagaca ccaccatcga gaagccgccg aggataaaca ccagcgtacg ataagagagc
    25201 ggtacgtact gggcaaagaa ttctgcacaa gcacaggtca ggccaaccgc cgtgaccaga
    25261 caggcgatga agattaacgc cgccagcagg aagctaccgc cgccgccaaa ggtgtgctga
    25321 acgtaagcat gcagaatagc agcgccgttt gcagactgat cgaccagcga cgcgctgtct
    25381 gaacccagac agaacagcgc caggtacagc aaagtcagac caacacccgc catcaggcca
    25441 gcccagacgg tataacgggt cagcagacgc gcttcggtaa cgccacgaga acgcgccgcg
    25501 ttaacaataa cgataccaaa caccattgcg cccagcgtat ccatggtcag atagccgtta
    25561 acgaagccgt tagaaaatgc agcgttttga taagcctcag tcgccgtgct gatagaaccc
    25621 gccggccaga caatcgcgac aacagacagg atgaccagcg cgataatttt cagcggcgca
    25681 aggaagttgc ccacggtatc cagcagcttg cccggataga gcgaaaccag aataacgata
    25741 gcgaaataga ccaggctgta gataaacagc ggcagcgcgg aatcacccgt cagcggcgca
    25801 atccccactt caaaggaaac ggtagctgta cgcggcgtag cgaaaagcgg ccccaccgcc
    25861 aggtaacaaa ccgttgccag cagtacgcca gcgactttac cgattggcgt gctgaggctg
    25921 tcaacaccgc cgccaacttt tgccagcgcc actaccgtta ataccggcag gccaacggca
    25981 gtaatgagga agccgaatgc cgcagtccag acgtgttcgc ctgcctgtaa gccgaccatt
    26041 ggagggaaaa taatgttacc tgcgccgacg aacaacgcaa atgtcataaa gcccagagcg
    26101 atgatatcgc gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg atgtggtgtt
    26161 gtaacgttaa atttttgtgc caatccctgg ggatgataca gcggtatatt taaacaattt
    26221 tagcgggaaa tactcccaac caggcgcggc gaagaatagt ttttcgattt accgcgttaa
    26281 tgcagtcagt atgacagcac ctgaagcgca atttaaacgc ttataacgtt ttaaagcaag
    26341 gagggatcta aaaaccagag gtatatagcg ataagaaatc accaaccagt ataaaacact
    26401 atttccgtga aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc gttgtaaaat
    26461 ctaacgcccg tattttgact gcaaagaaaa taagccagca tcggctggct tatggcaata
    26521 aaagatgaca aaggcggatt aatcgctgtt tttggcaatt aaacgttccg ggataacaaa
    26581 actgaaacgt gttccttttc ctactgtact ctcaatattc aggcgacttt cgtgatgatt
    26641 caccgcatgt ttcacgatcg ctaaccctaa tccgctaccg ccggtttgcc gggaacgcgc
    26701 tttatcaacg cgataaaaac gctcggtcag gcgcggaata tgctccggtg caatgcccgg
    26761 tccgttatct tcaacgctaa attcggcacc gtgcggcact cgctgccagc gtacggtgat
    26821 atgcgtgcct tccggcgtat gattcacggc gttatagacc aggttcgaaa tcgcactgcg
    26881 tagctgatct tcgttgccag acaccttgag gccgttatct atctcaaagg taaatgtctg
    26941 ttttttctga ctcagagtct gagcctcgcg ctcaacaacg cgcagcatca tcggcacatc
    27001 aaccttttca ttgagcaatt gcgtcggcgc ggcttcaatt ttcgacagcg ttagcaactg
    27061 cttcaccagc ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg ctttttcgcg
    27121 taccgcgcct tccagcggct gctcatccat catctccagg taaccctgta acacggtcaa
    27181 tggcgtacgt aactcatggc tcacgttggc aaaaaagtta cgccgcgccc cttccagttg
    27241 atgcatttgc gtgacatcac gcgccaccat cagcaactgt ttgtgggtat aaggcatgac
    27301 gcgaatttcc agatgccgcc cggtgttgag caccagattg agcgggcgag aaaaatcacg
    27361 cgttttcaga tattgcgtaa actccgggta acgcagtagg ttaaggatgt tctgcccgtt
    27421 atcttccggc cagcgcaaac caagaatttg ttgcgccaga ccgttacacc agaaaatacc
    27481 gccctcttcc gtggtcagca ccaccgcgtc gggcagcgac tccgcgccgc tacgaaagcg
    27541 tttaatcaga ttgcccagtt cacggcggcg ttttttattt cgcagctgca tctggtgtaa
    27601 gccgtatagt agcggttccc agctaccacg ccccggtggc ggggtcatac tgcgatccac
    27661 ccacagccac catgaaaggc gcaataaatt ccagaaatgc cagataagca gtcctgttac
    27721 cgatgccagc aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga aagccgggag
    27781 gcagcaaagt agcagctcca gcaccagcct tttccacgac agccgttcca gcacgtaaga
    27841 tactccagtt aagaaatcat aagccctgct ctgcgtccga tgagcaaggc gttaaaagcg
    27901 ggttgaaaaa cgatatcctg taccgcgcac ggtctgcacc atgcggtcat gcccgccggg
    27961 ctccagtgct ttacgcaggc gacgaatgtg gacatcgacc gtgcggtctt ccacatacac
    28021 gttagttccc cagacgtggt ttaacagctg ctcgcggctg tacacgcgct caggatgcgt
    28081 cataaaaaag tgcagcagtt taaattctgt cggccccatc tccagcggct cttcgcccgc
    28141 catcactcgg tgagatgtcg ggttgagact taatccctgc atcttaatca cctcttccac
    28201 cgccattggc gaaatacggc gcattaccgc tttgattcgc gccaccagct ccttcggcga
    28261 aaatggcttg gtgatatagt catccgcgcc ggtttcaagg ccgcgcacgc gatcttcttc
    28321 ttcccctctg gcggtcaaca tcaccactgg aatatcccgg gtcatcgact cgcgcttgag
    28381 gtgtttgatg aactggatac cggagccgcc aggtaacatc cagtcaagga gaattaaatc
    28441 cggccagggt tcattcagtt gattcacagc actgtcataa tcttccgctt cgaccggctg
    28501 aaagccattt tgttcgagca cgaagcagac catttcgcga attggagctt catcttctac
    28561 gaccagaata cgtctcgcca tgatttgccc tgttgtaata aataggttgc gatcattaat
    28621 gcgacgtcat tatgcgtcag atttatgaca gatttatgaa aagctcgtcg cacatatctt
    28681 caggttattg atttccgtgg cgcagaaaaa agcaaatggc acatctgttt gggtataatc
    28741 gcgaccatgc tttttcgcca gggaaccgtt atgcgcatcc ttcacacctc agactggcat
    28801 ctcggccaga acttctacag taaaagccgc gaagctgaac atcaggcttt tcttgactgg
    28861 ctgctggaga cagcacaaac ccatcaggtg gatgcgatta ttgttgctgg tgatgttttc
    28921 gataccggct cgccgcccag ttacgcccgc acgttataca accgttttgt tgtcaattta
    28981 cagcaaactg gctgtcatct ggtggtactg gcaggaaacc atgactcggt cgccacgctg
    29041 aatgaatcgc gcgatatcat ggcgttcctc aatacaaccg tggtcgccag cgccggacat
    29101 gcgccgcaaa tcttgcctcg tcgcgacggg acgccaggcg cagtgctgtg ccccattccg
    29161 tttttacgcc cgcgtgacat tatttccagc caggcggggc ttaacggtat tgaaaaacag
    29221 cagcatttac tggcagcgat taccgattat taccaacaac actatgccga tgcctgcaaa
    29281 ctgcgcggcg atcagcctct gcccatcatc gccacgggac atttaacgac cgtgggttcc
    29341 agtaaaagtg acgccgtgcg tgacatttat attggcacgc tggacgcgtt tccggcacaa
    29401 aactttccac cagccgacta catcgcgctc gggcatattc accgcgcaca gattattggc
    29461 ggcatggaac atgttcgcta ttgcggttcc cccattccac tgagttttga tgaatgcggt
    29521 aagagtaaat atgtccatct ggtgacattt tcaaacggca aattagagag cgtggaaaac
    29581 ctgaacgtac cggtaactca acccatggcg gtgctgaaag gcgatctggc gtcgattacc
    29641 gcacagctgg aacagtggcg cgatgtatcg caggagccac ctgtctggct ggatatcgaa
    29701 atcactactg atgagtatct gcatgatatt cagcgcaaaa tccaggcatt aaccgaatca
    29761 ttgcctgtcg aagtattgct ggtacgtcgg agtcgtgaac agcgcgagcg tgtgttagcc
    29821 agccaacagc gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa tcgccgtctg
    29881 gcactggaag acctggatga atcgcagcag caacgtctgc agcatctttt caccacgacg
    29941 ttgcgtaccc tcgccggaga acacgaagca tgaaaattct cagcctgcgc ctgaaaaacc
    30001 tgaactcatt aaaaggcgaa tggaagattg atttcacccg cgagccgttc gccagcaacg
    30061 ggctgtttgc cattaccggc ccaaccggtg cggggaaaac caccctgctg gacgccattt
    30121 gtctggcgct gtatcacgaa actccgcgtc tctctaacgt ttcacaatcg caaaatgatc
    30181 tcatgacccg cgataccgct gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag
    30241 cgtatcgcgc gttctggagc cagaatcggg cgcgtaacca gccggacggt aatttgcagg
    30301 tgcctcgcgt agagctggcg cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag
    30361 ataagctgga actgacagcg acgttaaccg ggctggatta cgggcgcttc acccgttcga
    30421 tgctgctttc gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg
    30481 aattgctcga ggagttaacc ggcactgaaa tctacgggca aatctcggcg atggtttttg
    30541 agcagcacaa atcggcccgc actgagctgg agaagctgca agcgcaggcc agcggcgtca
    30601 cgttgctcac gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta cttactgacg
    30661 aagaaaaaca gttaattacc gcgcagcagc aagaacaaca atcgctaaac tggttaacgc
    30721 gtcaggacga attgcagcaa gaagccagcc gccgtcaaca ggccttgcaa caggcgttag
    30781 ccgaagaaga aaaagcgcaa cctcaactgg cggcgcttag tctggcacaa ccggcacgaa
    30841 atcttcgtcc acactgggaa cgcatcgcag aacacagcgc ggcgctggcg catattcgcc
    30901 agcagattga agaagtaaat actcgcttac agagcacaat ggcgcttcgc gcgagcattc
    30961 gccaccacgc ggcgaagcag tcagcagaat tacagcagca gcaacaaagc ctgaatacct
    31021 ggttacagga acacgaccgc ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc
    31081 agttctccca acaaaccagc gatcgcgagc atctgcggca atggcagcaa cagttaaccc
    31141 atgctgagca aaaacttaat gcgcttgcgg cgatcacgtt gacgttaacc gccgatgaag
    31201 ttgctaccgc cctggcgcaa catgccgagc aacgcccact gcgtcagcgc ctggtcgcgc
    31261 tgcatgggca gattgttccc caacaaaaac gtctggcgca gttaatggtc actatccaga
    31321 atgtcactct ggaacagacg caacgtaatg tcgcacttaa cgaaatgcgc cagcgttata
    31381 aagaaaagac gcagcaactt gccgatgtga aaaccatttg cgagcaggaa gcgcgcatca
    31441 aaacgctgga agcccagcgt gcacagttac aggcgggtca gccttgtcca ctttgtggtt
    31501 ccaccagcca cccggcggtc gaggcgtatc aggcgctgga gcctggtgtt aatcagtctc
    31561 gattactggc gctggaaaac gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg
    31621 ggcaactgga tgcattaacg aagcagcttc agcgcgatga aaacgaagcg caaagcctcc
    31681 gacaagatga gcaagcactt actcaacaat ggcaagccgt cacggccagc ctcaatatca
    31741 ccttgcagcc acaggacgat attcaaccgt ggctggatgc acaagatgag cacgaacgcc
    31801 agctgcggtt actcagccaa cgacatgaat tacaagggca gattgccgcg cataatcagc
    31861 aaattatcca gtatcaacag caaattgaac aacgtcagca gcagctctta acggcattgg
    31921 cgggttatgc actgacattg ccacaggaag atgaagaaga gagctggttg gcgacacgtc
    31981 agcaagaagc gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg caaaaccgta
    32041 ttcagcagct gacaccgatt ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg
    32101 aagaaactgt ggcgctggat aactggcggc aggttcatga acaatgtctc gcattacaca
    32161 gccagcagca gacgttacag caacaggatg ttctggcggc gcaaagtctg caaaaagccc
    32221 aagcgcagtt tgacaccgcg ctacaggcca gcgtctttga cgatcagcag gcgttccttg
    32281 cggcgctaat ggatgaacaa acactaacgc agctggaaca gctcaagcag aatctggaaa
    32341 accagcgccg tcaggcgcaa acgctggtca ctcagacagc agaaacgctg gcacagcatc
    32401 aacaacaccg acctgacggg ttggctctca ctgtgacggt ggagcagatt cagcaagagt
    32461 tagcgcaaac tcaccaaaag ttgcgtgaaa acaccacgag tcaaggcgag attcgccagc
    32521 agctgaagca ggatgcagat aaccgtcagc aacaacaaac cttaatgcag caaattgctc
    32581 aaatgacgca gcaggttgag gactggggat atctgaattc gctaataggt tccaaagagg
    32641 gcgataaatt ccgcaagttt gcccaggggc tgacgctgga taatttagtc catctcgcta
    32701 atcagcaact tacccggctg cacgtgcgct atctgttaca gcgcaaagcc agcgaggcgc
    32761 tggaagtcga ggttgttgat acctggcagg cagatgcggt acgcgatacc cgtacccttt
    32821 ccggcggcga aagtttcctc gttagtctgg cgctggcgct ggcgctttcg gatctggtca
    32881 gccataaaac acgtattgac tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg
    32941 aaacgctgga taccgccctt gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg
    33001 gtgtgattag ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa
    33061 agatcaacgg cctgggctac agcaaactgg aaagtacgtt tgcagtgaaa taactattca
    33121 gcaggataat gaatacagag gggtgaatta tctcttggcc ttgctggtcg ttatcctgca
    33181 agctatcact ttattggcta cggtgattgg tagccgttct ggtggttgtg atggtggtat
    33241 gaaaaaagtc attttatctt tggctctggg cacgtttggt ttggggatgg ccgaatttgg
    33301 cattatgagc gtgctcacgg agctggcgca taacgtagga atttcgattc ctgctgccgg
    33361 gcatatgatc tcgtattatg cactggtggt ggtggtcggt gcgccaatca tcgcactctt
    33421 ttccagccgc tactcactca aacatatctt gttgtttctg gtggcgttgt gcgtcattgg
    33481 caacgccatg ttcacgctct cttcgtctta cctgatgctc gccattggtc ggctggtatc
    33541 cggctttccg catggcgcat tttttggcgt cggagcgatc gtgttatcaa aaattatcaa
    33601 acccggaaaa gtcaccgccg ccgtggcggg gatggtttcc gggatgacag tcgccaattt
    33661 gctgggcatt ccgctgggaa cgtatttaag tcaggaatgc tggcgttaca cctttttatt
    33721 gatcgctgtt tttaatattg cggtgatggc atcggtctat ttttgggtgc cagatattcg
    33781 cgacgaggcg aaaggaaatc tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg
    33841 gttaattttc gccgccacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt
    33901 aaagccatac atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat
    33961 gttagttggg ctagggatgg tgctgggaaa tatgctaagt ggcaggattt caggacgtta
    34021 ttcaccactg cgcattgcag cagtgactga ctttatcatt gtactggcac tgctgatgct
    34081 ctttttctgc ggcggcatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg
    34141 attatttgcc ctttcagcac ctctgcaaat attgttactg caaaatgcca aaggaggaga
    34201 gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata
    34261 ttgcggaggt atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc
    34321 cctgctttcg tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata agcgccagca
    34381 agcggcggat agtccggtgc tggcgaaacc actggggtag attactgtca cggtgcgcgt
    34441 tgcttattgc cggatgcggc gtgaacgcct tatccgccct acgcggttct ggcacatttt
    34501 gtaggcctga taagacgcgg caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt
    34561 ttttactctt gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc
    34621 gccttactca ccggcgtttc acattcgccg ccgaagacaa attgtttaat caactgccca
    34681 accgtttgat ataaacggtc tacattgctc atcccgcccc ccaggacaat cacatccgga
    34741 tcgagaatat tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat
    34801 gccagttccg ctaccggatc gctttcttca accaggcgga taatttcact gcctttcagc
    34861 gcatgtccgc tcaaacgacg ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca
    34921 atacaacctt gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg
    34981 tccatccacg gtagcggatt gtgtccccac tcacctgccg tgccattgcc gccgatatgc
    35041 gtccgcccat tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc
    35101 gtctgcgctc ccgctgccgc gccatctact gcttctgaaa ccgccagaca gttggcatca
    35161 tttgccagcc gcacttcccg ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg
    35221 ttgagccagg ttgaattgac attcttcacc acaccggtgt agggcgaaat cgaacctgga
    35281 atgcccatac ctaccgttcc gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg
    35341 gcgatcgttt caatagtctg ccggtaatca tcacgcggcg tgggcagacg atggcggtac
    35401 aactgctccc ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc taaatcgata
    35461 cctatacgca ctgtactctc cttatttttt tcaatatcaa tagcgtagag acggacaacc
    35521 ggattggcaa tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt taacgacaag
    35581 gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag
    35641 atttcgctgc gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc
    35701 ggcagccagg acatggcgaa gatgggctgg gttcctccga tgggatcgca cagcgatgcg
    35761 ttaacgcacg ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc
    35821 ccgtctccgg tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag
    35881 gcgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg
    35941 ctgccgcgtg ctttcagccg ttttagccag acaatgatgt ggctcgacac ggttaacggt
    36001 ttgattatgg tggactgcgc cagtgccaaa aaagcggaag atacgctggc attactgcgt
    36061 aaaagcctgg ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat tgaactgacg
    36121 ctgaccgaat gggttcgctc cggtagtgcg gcacagggct tccagctgct tgatgaagcc
    36181 gagctgaaat cgttgctgga agatggcggc gtgatccgcg cgaagaaaca agatctgacc
    36241 agcgaagaga tcaccaatca cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac
    36301 tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc
    36361 tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt
    36421 gatgccgatt tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa
    36481 ggattaggtg gcgaagcaca acgttaattg ctgattttcc tttaatgccg gatgcgacgc
    36541 ctgccgcgtc ttatccggcg tacgaagcca caccaggcat ataattattc gctacggcga
    36601 gcaataattt ttagcgcagc aatattatgc gttttacgcc gtaactgatc taatcgcctc
    36661 atccctattc gcctgtcagc agcacgcaga atattcacta acccccattt atcgctggta
    36721 aattgttttg ttaacagctc cgcttttggg gtatcaatat attcacgcaa taaatcagaa
    36781 attgaacaaa tatccgtcag gtaaggatac cagcaagttt caccgtgaaa attacgcact
    36841 ccagcgttac ctgcaataaa gtctttcggg taatcccaga tcacgttttt atccaacgtg
    36901 atccaataac gcggtaaatc ggtacttcca ccattttggc tgcgcatggg ataacgggta
    36961 cagtgaattt gcaggttgat cgttggcgtc agtaagtcat agatctcccg ctgtaacttg
    37021 ctccatggac gttgtgtcat tgtttttcct caagccggtg acgcgcttca ctgcgaattt
    37081 tccgcgttgc cccggttttc actaaatcac gtaactgcga aagatcgggt tgttcgacaa
    37141 ataagagtaa tgatggatcc tcttttaacc acgccgtttt cacatcggca gccagctgtg
    37201 gattattaat agccagcgac cgatcctgag gctcagtcaa tgtcgttaac aacgacgccg
    37261 gggtatgtgc gttgcgtaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg
    37321 cacgccacca ttcatcatct gatttatctt ttatcgtagc cagttccgca aaaaatacct
    37381 ggagatcttc actgatattt atatcaacct tgatgaatta tgaatatatc ggaaattgaa
    37441 aaaatcgcag caaaggaaat attattatgg gttaaagaga ggatatttca gaaaaatgcc
    37501 ctcccgttca aggggaaggc gaggaattac agatagcggc acagataaga ggtaggttcg
    37561 gcaacttgaa gatgaaactc actgtgaccg ggaacattaa acaccgaacc ggcttcatac
    37621 acttgccagt cggtcgcgtc aggcagtaac acattcagcg cgccactgat taccgtcatc
    37681 tcttccggct cagcggtgct gaaggtgtat tcgccttcaa ccataacacc cacgctggcg
    37741 cgaccagtgc tgctgctgga aaagccgatc gatttcactt tgccggaaaa gtactcatta
    37801 ctttgaagca taaactggcc ctgtctggta gttgaacatc tacttcacta taggggccag
    37861 aggcgctggc tgtcacgcaa aattacacca gtaattccgc agccagccgt gcaatcaata
    37921 cgttagagag caagacagga acatcgagct gtttttgcag taaatcgcga tgacgttggt
    37981 gaaatcctaa acaatccagc atgatgacat cagccccttt tgccagtaat tctttcccag
    38041 catcaatgat tttttgttct gaatcatgaa tggggttacc caatgaaaat accggcgatt
    38101 tctgcaaaat ttgccatttt tgcgcctgaa cgggcagcat ctcctcaacc ggaacgataa
    38161 cccccacctg atgatcttca acaatagagg aaaccagtgg aggcaatatt cgcgacggct
    38221 caagaaagat cgtattacgc gcagtcatac tactaatgtt tgctgtactc attaataaaa
    38281 tgacgtcata accctgatta tcgagcactt caaccacacc ttgcagatct cgctccactt
    38341 tacgacgcga aacatgggcc agctggttgt catttaataa tgtgagaatg gtgtcttcgc
    38401 ctgcttctgg cgcgtactcc gccatcactt cttcacgact taacttcccc agcaggctat
    38461 gatgggaaat attatcttcg tcaatgtatt ccgtcaggag cggcaaaacc tcctgcatag
    38521 gtacaatgcc gatggtcagg atcgccaacg acgcactcat tgttcatcca ccttactttt
    38581 ctttcgtcgt taattaccgg gcaagtgtga agcaccactg ctgacactta cttgtgctac
    38641 aaatgacaaa aagcgtagca gcagacgtgc acggcataat gtaaagattc caaatgattc
    38701 cagtaatgga atgttattgt ttaatattct aattattaga aaaacataaa ttatgaaaaa
    38761 tgtgacgcag atcccttgta gtggcactca gaatcccttc cgagcagtct gatgcggtca
    38821 ctctttgtca gggtcctcat agcgtttttt cgcatccatc gcttcctgag ccgtcggatg
    38881 ttcactgatc aacgagtctg gtttaggatg atcggctctg agttggtacc aggttaccgt
    38941 ctgtcctggc tttccttttt caatcgtcac tatatatgct tcacgaggat aaggcggttt
    39001 cgttggcatc gttcttcctt atttcacggg atgaacgtta agtataggcg ctctaaaatc
    39061 aacaattgat cgtctgtgcc agggcgctac gaatttcaga aatcacctgg ctgggttcgt
    39121 ttgttgcgtc gatgataata tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca
    39181 cttcctgaac ttcttcgctc agcggttttc ccgttaaggt tggccgtaaa tcttcttccg
    39241 gtgcagcttg cagtcggtta accaggactg atactggcgc acacaaataa accacgatcc
    39301 cgttattttg catgaagtga cgattaaatt ccgtcagaat aatgccgccg cctgtagcga
    39361 taacggtgga tgccgcagtt accgcttcca gcgccgccgt ttctctggcg cgaaatcccg
    39421 cccactcttc cctttcgacg atctccgcga ccgtcatatt gagctgtgat tgcaaccact
    39481 gatcggtatc gacaaaccga cggttaagcg aatcggcaag ggccattccg accgttgttt
    39541 taccacagcc ccgaggcccg atcagaaaaa gaggttgtgt catcgtgggt tttccccaat
    39601 aggtcgcaat gcggcgaaag ccggtgtcat gagaatagcg atcataccat caaactagta
    39661 caatttcgat tgtaaagaaa aaattccact taaagtaaaa atctcaatac atcccttact
    39721 ataccaataa atattcaaga atgaagtgta aataataaat tacatttagc cacgactacg
    39781 ttgcacttcc agccaccact tctccagctc cgcggcaaag gcctggcggt cacgttgtga
    39841 aaggctatct ggtccgccgg tctggatccc actggcacgt aaggtatcca taaaatcgcg
    39901 catcgtcagg cgctcacgaa tggtcgctgg cgtgtaacgt tcgccgcgcg aattaagcgc
    39961 cgctgcgcct ttctcgatgg cttcagcagc caaaggtata tctgcggtga tcaccaaatc
    40021 gcccgcttca cactgccgga caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg
    40081 cagcgtacga ataaatcgcg atggcggcac gcgtaaactc tggtttgcta ccagtaccag
    40141 cggcatctgc atacgttccg ccgcgcgata caaaatctct ttaattacat tgggacacgc
    40201 gtcggcatcc acccaaattg tcataaagtc atcctttgtt aggtaatcct ctattgtgtc
    40261 gcgcttttgc cttccggcat agttctgttt atgcttctgc cagcgattat caaaacaatg
    40321 aatttcacgg caggagtgag gcaatggaaa agaaaatcgg ttttattggc tgcggcaata
    40381 tgggaaaagc cattctcggc ggtctgattg ccagcggtca ggtgcttcca gggcaaatct
    40441 gggtatacac cccctccccg gataaagtcg ccgccctgca tgaccagttc ggcatcaacg
    40501 ccgcagaatc ggcgcaagaa gtggcgcaaa tcgccgacat catttttgct gccgttaaac
    40561 ctggcatcat gattaaagtg cttagcgaaa tcacctccag cctgaataaa gactctctgg
    40621 tcgtttctat tgctgcaggt gtcacgctcg accagcttgc ccgcgcgctg ggccatgacc
    40681 ggaaaattat ccgcgccatg ccgaacactc ccgcgctggt taatgccggg atgacctccg
    40741 taacgccaaa cgcgctggta accccagaag ataccgctga tgtgctgaat attttccgct
    40801 gctttggcga agcggaagta attgctgagc cgatgatcca cccggtggtc ggtgtgagcg
    40861 gttcttcgcc agcctacgta tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg
    40921 gcgggatgcc acgcgcccag gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa
    40981 aaatggtgct ggaaacggga gaacatccgg gggcactgaa agatatggtc tgctcaccgg
    41041 gaggcaccac cattgaagcg gtacgcgtac tggaagagaa aggcttccgt gccgcagtga
    41101 tcgaagcgat gacgaagtgt atggaaaaat cagaaaaact cagcaaatcc tgatgacttt
    41161 cgccggacgt caggccgcca cttcggtgcg gttacgtccg gctttctttg ctttgtaaag
    41221 cgccaaatct gccgatttca accactcacg atagtgactc atttgtgggt tcagcggcgc
    41281 aacccccaca ctaatccgta aagttacctg tggcgtattc ggcaagcgta acgtgtttag
    41341 cccttcacgc acccgtaaca tggcggtaat ggcgctctca gctggcgtac cggacatgat
    41401 tactgcaaac tcatcgccgc caaaccgacc aatcacatcg ctaccgcaca gggtaatttg
    41461 taactgtcgg gtaagcgcca caatcgcttc atcgcccaca tcatgacccc aggtatcgtt
    41521 gatgctcttg aaatggtcga tatcgataat cagtaacgtt gcatcgcgat tatgccgccg
    41581 acagttatca aattcattgc gtaacatagt ttcccaatga cgtcggttat acacgcccgt
    41641 catgccgtcg cgagtactca tgacctgcaa cctgcgttta tgttccgcca gtttggttgc
    41701 cgtctggtag ctgacccagc caaacatcag aggataaacg acaataatgg gaagggagag
    41761 ccaccattcc agcggagcac tattgaacga cacggtaatg cccgtaagct cgagggtgac
    41821 aaggcaggaa accaccatca acaccagacc cgcgacaaac agacgggggc cgcctgcccc
    41881 catcaaattc agacacagaa tcatcaacat agcggtggaa ggcagcacgt ttacgcccat
    41941 tacgcctacc cacattcccg ctaatactgc atcggttttt aagttgtaaa tttcccggct
    42001 aagcggatcg acggccctgc tcgctatctg ccaggctaaa tgcggccaga cgaacgccca
    42061 gccgaccaac accagccacc accagcccgg cggcgggtgc gaaaccagcg ttgaagcaat
    42121 cggtaagaac atgccagcca ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg
    42181 cagcccggac cgctgatgtt cgttttgagg agttaaagga ggttcctccc catgcgccgc
    42241 cgcttttttg aaaaagtttt catcattcat tatttttggg aacattcaga aacaattttc
    42301 ccaaattata gagacggggc taaaggcaga agtatgaaat ttcgggtgag ccactttcgt
    42361 agctcacccg ttagcact
//
LOCUS       DBBBSB010000022        41085 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.022, whole genome shotgun sequence.
ACCESSION   DBBBSB010000022 DBBBSB010000000
VERSION     DBBBSB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.022"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..131
                     /gene="yecU"
                     /locus_tag="ACIUS0_001154"
     CDS             <1..131
                     /gene="yecU"
                     /locus_tag="ACIUS0_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGB0218998.1"
                     /translation="ADITLKKQPLLFLAKLMVYSAALTFFTTNFHCNMTRKINEYA"
     gene            118..342
                     /gene="yecF"
                     /locus_tag="ACIUS0_001155"
     CDS             118..342
                     /gene="yecF"
                     /locus_tag="ACIUS0_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGB0218999.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(375..995)
                     /gene="sdiA"
                     /locus_tag="ACIUS0_001156"
                     /pseudo
     CDS             complement(375..995)
                     /gene="sdiA"
                     /locus_tag="ACIUS0_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(1225..1977)
                     /gene="tcyN"
                     /locus_tag="ACIUS0_001157"
     CDS             complement(1225..1977)
                     /gene="tcyN"
                     /locus_tag="ACIUS0_001157"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGB0219000.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(1974..2642)
                     /gene="tcyL"
                     /locus_tag="ACIUS0_001158"
     CDS             complement(1974..2642)
                     /gene="tcyL"
                     /locus_tag="ACIUS0_001158"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGB0219001.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2657..3643)
                     /gene="dcyD"
                     /locus_tag="ACIUS0_001159"
     CDS             complement(2657..3643)
                     /gene="dcyD"
                     /locus_tag="ACIUS0_001159"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGB0219002.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3748..4548)
                     /gene="tcyJ"
                     /locus_tag="ACIUS0_001160"
     CDS             complement(3748..4548)
                     /gene="tcyJ"
                     /locus_tag="ACIUS0_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0219003.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4636..5187)
                     /gene="fliZ"
                     /locus_tag="ACIUS0_001161"
     CDS             complement(4636..5187)
                     /gene="fliZ"
                     /locus_tag="ACIUS0_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGB0219004.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(5233..5952)
                     /gene="fliA"
                     /locus_tag="ACIUS0_001162"
                     /pseudo
     CDS             complement(5233..5952)
                     /gene="fliA"
                     /locus_tag="ACIUS0_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(6117..7769)
                     /gene="fliC"
                     /locus_tag="ACIUS0_001163"
     CDS             complement(6117..7769)
                     /gene="fliC"
                     /locus_tag="ACIUS0_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HGB0219005.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            8035..9441
                     /gene="fliD"
                     /locus_tag="ACIUS0_001164"
     CDS             8035..9441
                     /gene="fliD"
                     /locus_tag="ACIUS0_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGB0219006.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9466..9876
                     /gene="fliS"
                     /locus_tag="ACIUS0_001165"
     CDS             9466..9876
                     /gene="fliS"
                     /locus_tag="ACIUS0_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGB0219007.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            9876..10241
                     /gene="fliT"
                     /locus_tag="ACIUS0_001166"
     CDS             9876..10241
                     /gene="fliT"
                     /locus_tag="ACIUS0_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGB0219008.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10319..11806
                     /gene="amyA"
                     /locus_tag="ACIUS0_001167"
     CDS             10319..11806
                     /gene="amyA"
                     /locus_tag="ACIUS0_001167"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGB0219009.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(11840..12253)
                     /gene="yedD"
                     /locus_tag="ACIUS0_001168"
     CDS             complement(11840..12253)
                     /gene="yedD"
                     /locus_tag="ACIUS0_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGB0219010.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12440..13645
                     /gene="yedE"
                     /locus_tag="ACIUS0_001169"
     CDS             12440..13645
                     /gene="yedE"
                     /locus_tag="ACIUS0_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGB0219011.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            13642..13875
                     /gene="yedF"
                     /locus_tag="ACIUS0_001170"
     CDS             13642..13875
                     /gene="yedF"
                     /locus_tag="ACIUS0_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGB0219012.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13982..14652
                     /locus_tag="ACIUS0_001171"
                     /pseudo
     CDS             13982..14652
                     /locus_tag="ACIUS0_001171"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(14692..15900)
                     /locus_tag="ACIUS0_001172"
                     /pseudo
     CDS             complement(14692..15900)
                     /locus_tag="ACIUS0_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            15908..16334
                     /gene="tnpA"
                     /locus_tag="ACIUS0_001173"
                     /pseudo
     CDS             15908..16334
                     /gene="tnpA"
                     /locus_tag="ACIUS0_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            16911..17993
                     /locus_tag="ACIUS0_001174"
     CDS             16911..17993
                     /locus_tag="ACIUS0_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HGB0219013.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(18482..19280)
                     /locus_tag="ACIUS0_001175"
                     /pseudo
     CDS             complement(18482..19280)
                     /locus_tag="ACIUS0_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(19290..19841)
                     /gene="ybcL"
                     /locus_tag="ACIUS0_001176"
     CDS             complement(19290..19841)
                     /gene="ybcL"
                     /locus_tag="ACIUS0_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGB0219014.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(20010..20342)
                     /gene="emrE"
                     /locus_tag="ACIUS0_001177"
     CDS             complement(20010..20342)
                     /gene="emrE"
                     /locus_tag="ACIUS0_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGB0219015.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(20687..21001)
                     /gene="fliE"
                     /locus_tag="ACIUS0_001178"
     CDS             complement(20687..21001)
                     /gene="fliE"
                     /locus_tag="ACIUS0_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGB0219016.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            21215..22874
                     /gene="fliF"
                     /locus_tag="ACIUS0_001179"
                     /pseudo
     CDS             21215..22874
                     /gene="fliF"
                     /locus_tag="ACIUS0_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            22867..23862
                     /gene="fliG"
                     /locus_tag="ACIUS0_001180"
     CDS             22867..23862
                     /gene="fliG"
                     /locus_tag="ACIUS0_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGB0219017.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            23855..24541
                     /gene="fliH"
                     /locus_tag="ACIUS0_001181"
     CDS             23855..24541
                     /gene="fliH"
                     /locus_tag="ACIUS0_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGB0219018.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            24541..25914
                     /gene="fliI"
                     /locus_tag="ACIUS0_001182"
     CDS             24541..25914
                     /gene="fliI"
                     /locus_tag="ACIUS0_001182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGB0219019.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            25936..26277
                     /gene="fliJ"
                     /locus_tag="ACIUS0_001183"
                     /pseudo
     CDS             25936..26277
                     /gene="fliJ"
                     /locus_tag="ACIUS0_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            26274..27401
                     /locus_tag="ACIUS0_001184"
     CDS             26274..27401
                     /locus_tag="ACIUS0_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGB0219020.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            27506..27970
                     /gene="fliL"
                     /locus_tag="ACIUS0_001185"
     CDS             27506..27970
                     /gene="fliL"
                     /locus_tag="ACIUS0_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGB0219021.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            27975..28979
                     /gene="fliM"
                     /locus_tag="ACIUS0_001186"
     CDS             27975..28979
                     /gene="fliM"
                     /locus_tag="ACIUS0_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGB0219022.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            28976..29389
                     /gene="fliN"
                     /locus_tag="ACIUS0_001187"
     CDS             28976..29389
                     /gene="fliN"
                     /locus_tag="ACIUS0_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGB0219023.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            29392..29757
                     /gene="fliO"
                     /locus_tag="ACIUS0_001188"
     CDS             29392..29757
                     /gene="fliO"
                     /locus_tag="ACIUS0_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGB0219024.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            29757..30494
                     /gene="fliP"
                     /locus_tag="ACIUS0_001189"
     CDS             29757..30494
                     /gene="fliP"
                     /locus_tag="ACIUS0_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGB0219025.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            30504..30773
                     /gene="fliQ"
                     /locus_tag="ACIUS0_001190"
     CDS             30504..30773
                     /gene="fliQ"
                     /locus_tag="ACIUS0_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGB0219026.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            30782..31567
                     /gene="fliR"
                     /locus_tag="ACIUS0_001191"
     CDS             30782..31567
                     /gene="fliR"
                     /locus_tag="ACIUS0_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGB0219027.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            31852..32475
                     /gene="rcsA"
                     /locus_tag="ACIUS0_001192"
     CDS             31852..32475
                     /gene="rcsA"
                     /locus_tag="ACIUS0_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGB0219028.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(32519..32707)
                     /gene="dsrB"
                     /locus_tag="ACIUS0_001193"
     CDS             complement(32519..32707)
                     /gene="dsrB"
                     /locus_tag="ACIUS0_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGB0219029.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            32870..33097
                     /gene="yodD"
                     /locus_tag="ACIUS0_001194"
     CDS             32870..33097
                     /gene="yodD"
                     /locus_tag="ACIUS0_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGB0219030.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            33395..34210
                     /locus_tag="ACIUS0_001195"
                     /pseudo
     CDS             33395..34210
                     /locus_tag="ACIUS0_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(34207..35901)
                     /gene="dgcQ"
                     /locus_tag="ACIUS0_001196"
                     /pseudo
     CDS             complement(34207..35901)
                     /gene="dgcQ"
                     /locus_tag="ACIUS0_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(36072..36254)
                     /locus_tag="ACIUS0_001197"
     CDS             complement(36072..36254)
                     /locus_tag="ACIUS0_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGB0219031.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(36333..37250)
                     /locus_tag="ACIUS0_001198"
     CDS             complement(36333..37250)
                     /locus_tag="ACIUS0_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGB0219032.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            37423..38343
                     /gene="yedA"
                     /locus_tag="ACIUS0_001199"
     CDS             37423..38343
                     /gene="yedA"
                     /locus_tag="ACIUS0_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGB0219033.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(38332..38802)
                     /gene="vsr"
                     /locus_tag="ACIUS0_001200"
     CDS             complement(38332..38802)
                     /gene="vsr"
                     /locus_tag="ACIUS0_001200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGB0219034.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(38783..40201)
                     /gene="dcm"
                     /locus_tag="ACIUS0_001201"
     CDS             complement(38783..40201)
                     /gene="dcm"
                     /locus_tag="ACIUS0_001201"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGB0219035.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(40268..40769)
                     /locus_tag="ACIUS0_001202"
                     /pseudo
     CDS             complement(40268..40769)
                     /locus_tag="ACIUS0_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(40848..>41085)
                     /gene="drpB"
                     /locus_tag="ACIUS0_001203"
     CDS             complement(40848..>41085)
                     /gene="drpB"
                     /locus_tag="ACIUS0_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGB0219036.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        10239 a        10574 c        10118 g        10154 t
ORIGIN      
        1 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
       61 ccgccgcctt aacatttttc accacaaatt ttcattgcaa catgacgagg aaaataaatg
      121 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
      181 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
      241 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
      301 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
      361 cgtgcctttc agccggttat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
      421 gccgtccatt tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
      481 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
      541 aattgcagtt catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa
      601 aagcccagcg cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
      661 gcgtgcgcgg cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa
      721 tggccctgac taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc
      781 tgataataac taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg
      841 aatggtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga
      901 agctctattt catggtaggc ctcttctgcg gcctccatcc tctgaaaacg caacagcatc
      961 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
     1021 gagtatcatt tataatgata attcaaacta tcacataaat aagatttata tataatttat
     1081 attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt
     1141 tatgctgatt tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga
     1201 atcgtcgggg ctgatttttt cttattattg cagcagaagc ttctcgagga actggcgggt
     1261 gcgaggctgc tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc
     1321 ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt cgtgtgtcac
     1381 aatcaccatc gtgcgctttt cctgcgccag ctggcggatg gtgttcagga cttcgcccac
     1441 cagctctgga tccagcgccg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
     1501 cagtgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
     1561 ggtttcttta ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc
     1621 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg
     1681 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
     1741 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
     1801 cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg ttttgccgga
     1861 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
     1921 cagcaccgtc tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttt
     1981 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca
     2041 gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg
     2101 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
     2161 gacgtatctt ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc
     2221 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg
     2281 gcttcccact gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcggcatag
     2341 gcggcagtgt tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
     2401 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
     2461 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
     2521 gcgagaataa acccgagtag taagccaaaa aacatgccgc cgatactgag ttgcagggta
     2581 tacccagccc ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc
     2641 atgagcgttc ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg
     2701 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca
     2761 gccccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agacgcgcca
     2821 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
     2881 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
     2941 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca
     3001 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
     3061 tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt
     3121 ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga
     3181 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ttcttccagt tgggcattgg
     3241 gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat
     3301 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
     3361 agtgcagacc gagtttcgcc gcgactgcgg cagtctggcg cacatggtta gactggatcg
     3421 cgccggcagt aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca
     3481 gcttacgtaa tttattgccg cccattgcca tgggggtgac ctcatcccgt ttgatgaaaa
     3541 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
     3601 taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
     3661 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
     3721 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
     3781 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
     3841 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
     3901 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
     3961 aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg ggtcatcatc
     4021 ataggtacgc acatcgacac cctgaacatt ctgccgcagc cactcttcat agttggtgcc
     4081 cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt
     4141 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
     4201 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
     4261 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
     4321 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg
     4381 aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc tctctttgac
     4441 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac
     4501 ggccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa
     4561 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
     4621 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
     4681 tccggtacag gtttggcgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
     4741 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg
     4801 atcgagggaa atattttgct catgtagatg attacccaaa cggcgcagac gaacgacata
     4861 ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaatttct taaagccgat
     4921 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
     4981 cgcccatgat tgttgttccg tttgccagcc tttttcatca agcagcgtgt ccaggcggga
     5041 aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat cgagtaattt
     5101 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
     5161 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg
     5221 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgt cctggctgtg
     5281 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
     5341 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatggc
     5401 ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
     5461 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcacgccact catcgtagga
     5521 gaagagctgg ctgttattgg tgtcgagcag catttgacga taatcggcaa tatcgatccc
     5581 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
     5641 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
     5701 gcgaagttca tccagcatag cgccacggat acgctacact gcgtaagttg taaatgccgt
     5761 tccttgtagg gcgtcatagc gttcgacagc attaagtaac ccaatgccgc ccgcctgtag
     5821 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
     5881 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
     5941 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
     6001 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttacgca
     6061 aaaagccgaa tgtaaaaaac cccgccggtg gcggggtttg agaggcattc agacgattaa
     6121 ccctgcagca gagacagaac ctgctgcggt acctggttgg cttttgccag cacggagtta
     6181 ccggcctgct ggataatctg cgctttcgac atgttggaca cttcggtcgc atagtcggcg
     6241 tcctgaatac gggactgcgc ttcagacagg ttggtagtgg tgttgttcag gttggtgact
     6301 gcggaatcca aacggttttg caccgcaccc agggaggagc ggaatttgtc gatggagctg
     6361 atggcttcat ccagagcttt caggggatcg gctgtcgtag ctgcttttgt ttcagcatct
     6421 gtcgtcaatt taccatcagc atctttataa actgccttac cgctgccgtt agtcactgaa
     6481 ccatcatcct gtaggtaaaa atctacagta tcagatgtgg tcaacgcctc gttgttatca
     6541 acataaaccg aatcaacacc ttttgttaca gcgccagttg tgccgttgac gctataacct
     6601 gcagtagtga aatcaacatt gtttcccgag gttgatacag ctttatcgaa agctgcctga
     6661 tcaacttttg cacctgttac tgaataagta atagtgtccg gcgtagtact attaccggta
     6721 aaggagatag aggtaccgcc aatgtcaatg gttgtcgctt tggtattatt ggctgcaagt
     6781 gtggacagac tagcctcagt caaagaagca ccagagccgt ttttcgttaa gcgcccagtt
     6841 gtatcaatgt agagtttatc tccattgctt gccgtaattt taccatcact gctgatctga
     6901 acatcctgtg cagaaccatc gatagtaata gtgcccttag cggtatcacc aactcccggg
     6961 gtcaaatatt tctgaacatc ggcagctgaa gccgttgtgg tatcaaaaga gtatgactta
     7021 cttgcactgt catacttgta attcgttgca ctagccgccg ttccgaagcc gttattaatg
     7081 gttgcctgaa cagtatcacc atcactaact ccagccagca aatcagacgc tttagcagca
     7141 tcgtaacccg cactcacagt atatttgttg ccgaccactg ttgcattagc agctaccaag
     7201 tcagctttag atgcagcagt attagccaca gccccgccac cattcacatt aaacccattc
     7261 agccccagcg tatcagagtc aattttcttc agatcaatag tgatagtctg gccgtcattc
     7321 gcaccaacct gaattttcat cgaaccgtct tttgccagta cgttcacgcc gttgaactgg
     7381 gtctgaccgg atacgcggtc aatttcgtcg agacgggatt tgatttcgtc ctgaatggag
     7441 tccagatccg aatcagagtt agtcccggta gaagcctgaa ccgtcagctc acggatacgc
     7501 tgtaagttgt tgttgatttc ggacagcgcg ccttcagtgg tctgtgcaac agaaataccg
     7561 tcgttggcgt tacgtgcagc ctgagtcagg cctttaatgt tagaagtaaa acggttagca
     7621 atcgcctgac ccgcggcgtc atccttcgcg ctgttaatac gcaagccaga agacagacgc
     7681 tcgatagaac tcgacagcgc agactggttc ttgttgatat tattttgagt gatcagcgag
     7741 aggctgttgg tattaatgac ttgtgccatg attcgttatc ctatattgca agtcgttgat
     7801 tacgttttgg gtttccaccc gtcggctcaa tcgccgtcaa ccctgttatc gtctgtcgta
     7861 aaacaacctt tagaattttt ttcacaaaca gccatttttt gttagtcgac gaaatactct
     7921 tttctctgcc ccttattccc gctattaaaa aaaacaatta aacgtaaact ttgcgcaatt
     7981 caggccgata accccggtat tcgttttacg tgtcgaaaga taaaaggaaa tcgcatggca
     8041 agtatttcat cgctgggagt cgggtcaggt ctggatttaa gttcaatcct tgatagcctc
     8101 accgccgcgc aaaaagcgac gctaacccct atttcaaatc agcaatcgtc gtttaccgct
     8161 aaacttagcg cctacggtac gctgaaaagc gcgctgacga ctttccagac cgccaatact
     8221 gcattgtcta aagccgatct tttttccgcc accagcacca ccagcagcac caccgcgttc
     8281 agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt cacccatctg
     8341 gcgcaggcgc aaaccctgac cacgcgcacc accagagatg atacgaaaac ggcgatcgcc
     8401 accagcgaca gcaaactcac cattcaacaa ggcgacgaca aagatccgat taccattgat
     8461 atcagcgcgg ctaactcatc gttaagcggg atccgtgatg ccatcaacaa cgcaaaagcg
     8521 ggcgtaagtg cgagcatcat taacgtgggt aacggtgaat atcgtctgtc agtcacatca
     8581 aatgacaccg gccttgataa tgcgatgaca ctctcggtca gcggtgatga tgcgctacaa
     8641 agttttatgg gctatgacgc cagtgccagc agcaacggta tggaggtctc ggtcgccgcc
     8701 cagaatgcgc agctgacggt caacaacgtc gccatcgaga acagcagcaa cactatcagc
     8761 gacgcgctgg aaaacatcac tctgaacctg aacgatgtca ccacgggcaa ccagacgcta
     8821 accatcactc aggacacctc caaagtgcaa acggcgatta aagactgggt gaatgcctat
     8881 aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga tgcgggagct
     8941 gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg gacgattcag
     9001 acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa aacgctggcg
     9061 cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc cgacaaactc
     9121 accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa
     9181 aaaaccggca tcacaaccac catcggcagc aacctgacca gttggctttc gacaacgggc
     9241 attattaaag ccgctaccga tggcgttagt aaaaccctga ataagttaac taaagactac
     9301 aacgccgcca gcgatcgcat tgatgcgcag gtcgcgcgct acaaagaaca atttacccaa
     9361 ctggacgttt taatgacctc gttaaacagc accagcagct acttaacgca gcagttcgaa
     9421 aacaacagta attccaagta atcattattc atcgggagac aggtcatgta cgcggcaaaa
     9481 ggcacccagg cctatgcaca aattggcgtc gaaagcaccg taatgagcgc cagccaacag
     9541 cagttggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc gcgactgttt
     9601 atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat caacatcatt
     9661 gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaattaac ccaaaactta
     9721 attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg caacgatgtc
     9781 tcagcagtcg aagaggtgga agcattaatg cgtaatattg ccgatgcctg gaaagagtct
     9841 ttactctccc cttcttcgat tcaggaccca gtctgatgaa caatgcaccg catttgtatt
     9901 tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca acggaagaac
     9961 aatgggacga actcatcgct agcgaaatgg cgtatgtgaa tgcggtgcag gagatcgcac
    10021 acctgactga agaggttgcc ccgtccacta cgatgcagga gcagctccgc ccgatgctgc
    10081 acctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg atggatgaac
    10141 tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc
    10201 agggcggctt tgtgctggct ccgcaggata acctcttttg aatcttaatg agccgatggc
    10261 tcgcgaataa tccgattacg gctacgcttc taatattccc cttgaatgga gtcgaagaat
    10321 gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg
    10381 gcctgaactg gccgagcgcg cagacggttt taatgatatt ggtatcaata tggtctggtt
    10441 gccgcccgcc tataaaggcg catcgggcgg ctattcggtc ggctacgact cctatgattt
    10501 atttgatcta ggtgagtttg atcagaaagg cagcatccct actaaatatg gtgataaagc
    10561 acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc tgctggacgt
    10621 ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc agcgtgtaaa
    10681 tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag tgtgaaggct ggacgcgtta
    10741 caccttcccc gctcgtgccg ggcaatactc gcagtttatc tgggatttca aatgttttag
    10801 cggtatcgac catatcgaac accctgatga agatggcatt tttaaaattg ttaacgacta
    10861 taccggagaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg attatctgat
    10921 gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg
    10981 ctgggtgatg gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca aacatattcc
    11041 ggcatggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt
    11101 tattgtggct gaatgctggt cgcatgaagt cgataaactg caaacgtata ttgatcaggt
    11161 ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg aagcatcgcg
    11221 catgggtcgc gactacgaca tgacgcagat cttcactggg acgctggtgg aagccgatcc
    11281 ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc
    11341 accggtcgaa ccgtggttta aaccgctggc gtatgcctta attttgttgc gggaaaatgg
    11401 cgttccttcg gtgttctatc cggacctcta cggtgcacat tacgaagatg tcggtggtga
    11461 cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc
    11521 ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga actgcattgc
    11581 ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacggaga
    11641 tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct ggcgtgattt
    11701 tctggggaac cggcaagaga gcgtagtgac agacgaaaac ggcgaagcga ccttcttttg
    11761 caacggcggc agcgtcagcg tgtgggttat cgaagaggtg atttaaatcc atccccggcg
    11821 gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg
    11881 cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg
    11941 tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta gtgagatcat
    12001 cagacatcaa tgtcaactta cccggcacgg caatcccccg ctgccactga cggcaatcca
    12061 aggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccggactg accagcgcac
    12121 gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga
    12181 cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg
    12241 cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagctct
    12301 gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat
    12361 tattaccccg aaaataaatt tgcatggtgt atgattcgcg gctcttgttt caaaccttct
    12421 gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt
    12481 ctgggcaccc atccctgcgg tcatcgcggc gggtattctc tccacttact attttggcat
    12541 tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc agctcctgca
    12601 gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc
    12661 gccattaacc cggatcgacg gcatgatgat cctcggtatg tttggcggct gctttgctgc
    12721 tgcgctgtgg gccaacaatg tcaaactacg catgccacgc agccgtatcc gcattatgca
    12781 ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg gctgtaacct
    12841 ggcggcgttc tttaccggca ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc
    12901 cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc
    12961 cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg
    13021 gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg cgctgctcac
    13081 ggcgatgaac caaccaaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact
    13141 gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg
    13201 tacccatatg gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg ggatcttcag
    13261 ttacgtacag ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg cggtaattgg
    13321 tggtttactt tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg gctggatgta
    13381 ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaacg tgatcggctc
    13441 aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa
    13501 aatcaacctg ctgaaaactt ttggtccgat gggaggcctg ctggtgacat atttgctgct
    13561 gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgaggcacc
    13621 gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct tgatatggtg
    13681 ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca attaaaaaaa
    13741 ggtgagatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat tccactggat
    13801 gcgcgtaatc acggctacac ggtgctggat attcagcaag acgggccgac cattcgttat
    13861 ttaattcaga agtaatccct cattcccgct gtggcccaca gcgggaacat cgttacctct
    13921 gacacataga ttttacccgc cccctctcct attcttttca gtatgatctc aggaggttgc
    13981 tatgtgtgga cgctttgccc aatcccaaac gcgtgaggat taccttgcgc ttctcgcgga
    14041 agatattgaa cgcgatattc cctacgatcc cgaacccatt ggcagataca atgtcgcgcc
    14101 gggaaccaaa gttcttctgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt
    14161 ctggggatat gctcccggat ggtgggataa accgccgctg attaacgccc gcgtagaaac
    14221 tgcctcaacc agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc
    14281 cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa agccttattt tatctatcgc
    14341 gctgacggac aacctgtttt tatggccgcg ataggtagca taccgtttga gcgtggtgac
    14401 gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt agatattcat
    14461 gaccgccgcc cgctggttct gtcgccagaa gctgcacggg aatggatgcg gcaagacatt
    14521 ggtggtaaag aagcctcaga aatcgctacc agaagctgtg tgccagcaaa ccagttcacc
    14581 tggcaccctg tgtcgcgcac ggtgggtaat gtcaaaaacc agggcgcgga gttaattcaa
    14641 cctgtttgtt gacatcctcc acgccctgaa tgacgaggac ccctgctacg ttcaggctgt
    14701 cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc
    14761 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gctccattta catcggcgtt
    14821 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat tttctttcgc
    14881 tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag caagcacctg
    14941 accgccacgc cagagctgct tgtactcaag ctggcggcgc atttcatacc agccctgatc
    15001 cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt
    15061 acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta tcattacgtg
    15121 gtttttgctg acggtcgttg tgactttgtg aaggtagtcc ctgcggatat ttgcgataca
    15181 ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttgttgctga atttgacctt
    15241 gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa aactgtttac
    15301 aggctcaaag actgtgccat ctgacagcga ggcaagttta gccacgccag catccagccc
    15361 gaccattgat gctgaagggt gaaccggagt tgatacttca ctttctgcct gaatactgat
    15421 gtaccactta ccgcaggact ggctgacagt gacatttttc acaacacccg tgacctgacg
    15481 gctgttccgg tagcgcatcc agccaagttt tggcagaaaa atacggctgt tttcctgagc
    15541 gagcttaacg ccctgcggat agcggaatgc atcattctgt caccgctttt tgaatcaggg
    15601 gaacgccgag cgcttctgga aaaagttttt gcaggcctgc tcagggtctt tcagtgactg
    15661 ttgcaatggc tgtgacggag catctttaat ccattgcgtt tcagtggcat ttttccactc
    15721 aaccaatcag gaagccattt tcgcgtaagg gatgtctttg ttaccggcct tatgattctc
    15781 attctgacgt gccagagtac gactgaaaac gaaacgacat gcgcctgcga agcgcctcat
    15841 ttcacgcgcc tgttgaccac cgggtcttaa ccggaatttg aatacctgta gtcgtttcac
    15901 agggcaaatt atactttggt ctgtgaaaaa aactgatatc cgacgcggaa ggcactgtgt
    15961 tttcctgatg catgctcacc gggtctttgt taccaagtac cgacgccaga tttttgacca
    16021 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
    16081 actggttgaa atggatggcg aaccagatca cgtccatctg ttaattaact atccccccaa
    16141 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
    16201 gacgggatcg accagatatt gctgccaggt attaccgcaa agatgttttg tggcgtcccg
    16261 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
    16321 aacagcaaac acctggtcag atgaaaaaac gagccttata tccccgccct gaaggacggg
    16381 cttttacggt gcactggata agcctggaga ttatctacac ctgcatggac atcattgtac
    16441 tatgccccgt acatgctcca tcacatcctc aacatcaaag tattagagcc ttgcaaacca
    16501 ttatgcgtct cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg
    16561 gcgctatatt gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat
    16621 catgcaaata atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag
    16681 tcaatcataa ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa
    16741 atagtcatgc attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa
    16801 tttaaatgag aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg
    16861 tagtgcagtg acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat
    16921 taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag
    16981 cagagattta caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc
    17041 actacttctc ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca
    17101 aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca
    17161 aaggcaaccg tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg
    17221 gcctgaaatt cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg
    17281 atatcggtgc atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag
    17341 atgtgttcat gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg
    17401 gtctggttga tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg
    17461 taactgaagc taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg
    17521 gtgtaggtgc aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca
    17581 acagcctgaa tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg
    17641 cgaacaacat ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta
    17701 ataaccatat tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact
    17761 tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg
    17821 gtgatcagga cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata
    17881 tgtccacttt tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat
    17941 ctggcgttgc taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt
    18001 actcaaagat atgttgtggg aggctttgcc tccccaacat ataagtggcg ctctcaagcc
    18061 actttcctta gaagcactat cttgcttctg actatataaa ttacccttta ttttctggga
    18121 cgtttcaacg ccccattttt aataatactc agtgaacaat taacgtatca attagattta
    18181 ataacaacga tatccgtacc taacctgata tctaatgtca taagcatccc tttaattatc
    18241 ccctcaacct cttgtaaatt tcccactctt gagctccaca tagcccaaac gccggatgca
    18301 aaaaaaatca tacgtgcatc tctgaaattg tgatggagga agtgagtatc aattatatgt
    18361 gcttataaac gttcccgatt ggcacagaag tcaccggatt gttcaggctc caataggttg
    18421 agtatgtcgt atttattttc caaaataaaa atcgatgctg ttgctcctta ataaaatact
    18481 tttactctct ggacgctttt cttagctctc ttggtgttat attaaaacga ttatggaacc
    18541 tttcagtaaa acgagaagga cacttgtaac cattttccct ggcaatctcg ctgatgggtt
    18601 ttactgtcgt ttgtatagca ggacaacgca ttatttaacc tcacgtcgtc caatatactt
    18661 tgaaaactta ttccctcgct tgccagacgg cgatgcaatg tggaaacaga aatgtagaga
    18721 tatctcgcta ccttgtttgc tgtccatttt gttctaggtt cggatagcat taggttataa
    18781 caacgactta ttaatgattg tttactatct gataaaagta aacaattaac atgatgtgct
    18841 cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa tttgcgttga gaagcctcgg
    18901 gaaatagatg tttttagttg ctcccaacaa tatattaact cggggttctg aggtaagcag
    18961 aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa atttttgaaa cttttcaatt
    19021 agatcaatgg gataataaag catttctgca agataaagac ctgcttctgg ataattcaca
    19081 atataaaatt catatccaca gggaaataat attatctgat tattatcaac agttagacta
    19141 tgcgtctccc aattgataac tttttttccc tgacggatac gacataaagc tggcataaga
    19201 ggcttaaccc tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta
    19261 gacttatctc ttgccagcat actcttaccc tattttatct cataaactgg tgttatctca
    19321 gcggttgcaa ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa
    19381 tccacaggaa tcttatcagt ttttagagcc catactttaa actggtaatg atggggttta
    19441 tctcctttgg gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga
    19501 acagcaccag tcggaagttt tgtaccatca cgtcttcctg catcagtggg caaatatgtt
    19561 acagttgctg gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct
    19621 ggatcataca cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaggttaat
    19681 gagggcgatg tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt
    19741 tgctctcctg tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat
    19801 gttatgaatg ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga
    19861 cctatcgtta ttttatttga tgtgccttga tctcattatg cataaaggcg caatgatcgc
    19921 gcaaatgcaa tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag
    19981 attcctgtta tctcaaggag aattattttt taatgcggtg cacttcgtga caacaagtta
    20041 atgactaaca caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt
    20101 tgaccaaaaa atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata
    20161 gcataagcaa tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca
    20221 caatagcaaa taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc
    20281 attaaggttg taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc
    20341 atagcatatt ctttcctgtt catactggag agaattgtac tacagtttga acacaactca
    20401 ccgttttcat cctggtgtat gtgtatccaa ggggctctgg cgtggcaaga gataaaggca
    20461 ggaacaataa tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc
    20521 aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aacaaccagg
    20581 atcaggtatc cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg
    20641 tgggatcgga tataattaaa caagcatagt caacaggtta cacaacctac acctgcatgg
    20701 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
    20761 aaactgaggc tttttgcata tcggtcatca catcgtttag cgccacgccg ggttcaccga
    20821 gggtgaattt ttctgcctgc gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg
    20881 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg
    20941 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
    21001 ttttcgtctc ccggataatt tctggtaaca aagcctacca gtaagtcaat aagacaaagg
    21061 cgctaaatag caacaaaaaa actggtttat tggcggatag aaaaaacgaa agcacaaata
    21121 atgggagcgt caatttttcg agtgtgctga cccgagagtg agtcttgttc cactttgcca
    21181 ataacgccgt ccataatcag ccacgaagtg cgcgatgaat gcgactgcag cccagacaaa
    21241 atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg
    21301 ttccgcggca gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg
    21361 cacattattc agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca
    21421 aatgaatatt ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa
    21481 agttcacgaa ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga
    21541 actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg
    21601 ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg
    21661 cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc
    21721 atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat
    21781 tgtacatctg gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga
    21841 tcagggcggg catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca
    21901 gttgaaatat gccagcgatg tcgaaggccg tattcagcag cgtattgaag cgatcctgtc
    21961 acctattgtt ggtaacggta atattcacgc ctaggtcacg gcgcaactgg acttcgccag
    22021 taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg
    22081 ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg
    22141 ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa
    22201 tcaaaataac cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca
    22261 gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt
    22321 gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg
    22381 taaaccattg cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc
    22441 gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag
    22501 cagtgacgaa agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt
    22561 gctggctgcc ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg ttgctgtggc
    22621 ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac
    22681 aggcgcaggc ccgcgaggaa gtggaagatg cggtggaagt ccgtctgagc aaagacgaac
    22741 aactccaaca acggcgcgct aaccaacgac tgggggcaga agtcatgagc cagcgtatcc
    22801 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
    22861 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
    22921 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc
    22981 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag
    23041 tttgagcaag aagctgaaca gtttgctgca ctgaatatca acgccaacga ttatctgcgt
    23101 tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga agatattctc
    23161 gaaacccgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc
    23221 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgagg
    23281 cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
    23341 ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggccgaact gaccgaagta
    23401 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg
    23461 gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag aagccgttat taccgccgtg
    23521 cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg agatgttcct gttcgagaat
    23581 ctggtggatg tcgatgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
    23641 ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
    23701 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca agcgtggtcc ggtgcgtctg
    23761 tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct tgccgaaact
    23821 ggcgagatgg tgattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
    23881 ctggacgccg gacgatctcg cgccaccacc ggcagagttt gtgccaatgg tcgagtcgga
    23941 agaaaccatt attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat
    24001 gcaggcccac gagcagggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcacga
    24061 acagggctat caggaaggac tggcccaggg gctggaacaa ggtctggcag aggcgaaggc
    24121 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
    24181 tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag atggcgctgg aggcagcacg
    24241 tcaggtgatc ggtcagacac caacggtgga taactcggca ctgatcaaac agatccaaca
    24301 gttgttgcag caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac acccggatga
    24361 tctgcaacgt gtggatgata tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg
    24421 gggcgatccc accctccacc ctggcggatg taaagtctcc gccgatgaag gcgatctcga
    24481 cgccagtgtc gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta
    24541 atgaccacgc gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg
    24601 cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa
    24661 gccaccggat tacaattgcc gctcggcgca acctgtgtca ttgagcgcca gaacggcagc
    24721 gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg
    24781 ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa aaacatttcg
    24841 gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt
    24901 ctggacggta gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac ggaaaccggt
    24961 gcgctgatta ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac
    25021 accggcgtgc gcccaatcaa tgccctgctt accgtcggac gcgggcagcg tatggggctg
    25081 tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg ctacacccgc
    25141 gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa agattttatt
    25201 gagaatatcc tcggtgccga aggacgtgca cgctcagtgg tgattgccgc tccggcggat
    25261 gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt
    25321 cgcgatcgtg gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc
    25381 cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg
    25441 gtgtttgcca aattaccggc actggtcgag cgtgccggta atggcattag cggcggcggc
    25501 tcgattaccg cgttttatac cgtgctcacc gaaggcgatg accagcagga ccccattgct
    25561 gactccgcgc gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc
    25621 gggcactatc cggctatcga tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc
    25681 agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt
    25741 aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgataaa
    25801 gccatcgccc tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg
    25861 gactgggaag cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg
    25921 agataacggc agatggcgga agaacagctc aaaatgttga tcgattatca gaatgaatat
    25981 cgcaataacc tcaatagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat
    26041 cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag
    26101 tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag
    26161 gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc
    26221 gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc
    26281 gcttagcgcc cttgattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca
    26341 gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc attagcaggc gagacaacaa
    26401 ctgacaaagc ggccccccag ttgctggtag caacagataa gcccacgaca aaaggcgagc
    26461 cgctggtcag cgatattctt gccgatgcgc aacaagcgga tttactgatc cctgtggatg
    26521 aaacacttcc tgtcatcaac gacgaacaat ccacatcaac accgttaacc accgctcaga
    26581 cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc
    26641 ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc gatgttgccg ggttttgaca
    26701 atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgctct
    26761 tcacaaaact gacttctgcg caactcacta cagcacagcc tgatgacgcc cccggcacac
    26821 cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca
    26881 cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac tccacaccag acacagccac
    26941 tgcccaccgt cgccgcgcct gttttgagtg caccgctggg ttctcacgaa tggcaacaat
    27001 cattgagcca gcatatttcg ctgttcaccc gccaggggca acaaagtgca gagttgcgtc
    27061 tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc
    27121 agatccagat gatttcaccg catcagcacg tacgcgccgc cctggaagca gcgctgccgg
    27181 tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac attagtggcg
    27241 aaagctttag tggtcagcag caggccgctt cccagcaaca gcaaagccaa cgcacagcaa
    27301 accatgaacc tctggcgggg gaagacgacg atacgcttcc ggttcccgtc tctttacaag
    27361 ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta
    27421 atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag
    27481 tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg
    27541 atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta
    27601 cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc
    27661 ctccccggtc ttctatgcgc tggatacctt tacggtcaat ttgggcgatg cggatcgcgt
    27721 gctttatatc ggcataaccc tgcgactgaa agatgaagca acccgctcgc ggctgagtga
    27781 gtatttgccg gaagtccgta gtcgcttgct gttactgttt tcacgtcagg atgctgccgt
    27841 actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca cactttccac
    27901 cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat
    27961 tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga
    28021 atggtgacag cgaagtcaaa gacgaaccga cagccagtat tagcggcgaa agtgacattc
    28081 gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa
    28141 tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgccgta
    28201 gcccggacat taccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc
    28261 tgccggtgcc gaccaacctg aacctaatcc acctgaaacc gctacgcggc acagggctgg
    28321 tagtgttctc gccgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac
    28381 gcttcccgac caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc gtcatcaacc
    28441 gtatgttgaa gctggcgctg gaaggctata gcgacgcctg gaaagcgatt aatccgctgg
    28501 aagttgagta cgtgcgttcg gaaatgcagg tggaatttac caatatcacc acctcgccga
    28561 acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta
    28621 atatctgcct gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc
    28681 tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc
    28741 attcacagct ggaactggtg gccaactttg ccgatatctc gctacgcctg tcgcagattt
    28801 taaaactgaa acccggcgac gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg
    28861 ttgacggcgt cccggttctg accagtcagt acggtactct caacggtcag tatgcgttac
    28921 ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag
    28981 tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc
    29041 gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt tccagcaatt
    29101 tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt
    29161 caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac
    29221 gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata ttctgatcaa
    29281 cggttattta atcgcccagg gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat
    29341 caccgatatc attaccccgt ccgagcggat gcgccgcctg agccgttagt gatgaataac
    29401 catgctactg tgcaatcttc cgcgccggtt tctgctgagc cactgctgca ggtgagcggc
    29461 gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggctggga
    29521 tttgccccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc
    29581 gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc
    29641 gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg
    29701 cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag atcctgatgc
    29761 gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat tacgcccctc gccttcgcgc
    29821 aactgccggg tatcaccagc cagccgctac ctggcggtgg acaaagctgg tcgctcccgg
    29881 tgcagacgct ggtgttcatc acctcgttga cgttcattcc agcgatttta ctgatgatga
    29941 ccagtttcac ccgcatcatt attgtttttg gtttattgcg taacgcgctg ggaacgccct
    30001 ccgcgccacc taaccaggta ttgctggggc tggcactgtt tttgaccttt tttattatgt
    30061 cgccagtgat cgacaaaatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatct
    30121 caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg ctgcgtcaga
    30181 cccgcgaggc agatttaggg ttgtttgcca gactggcgaa taccggcccg ttgcaggggc
    30241 ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag ttgaaaaccg
    30301 catttcagat aggcttcacg attttcatcc cttttttgat tatcgacctg gtgattgcca
    30361 gcgtgttgat ggcattgggg atgatgatgg ttccccccgc caccattgct ctgcccttta
    30421 aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg ctggcgcaga
    30481 gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg ggaactgaag
    30541 cgatgaaagt cgcgctggca ctggcagccc cgctgttgct ggtagcgctg gtcacgggcc
    30601 ttatcatcag tattttgcag gcagccacgc agattaacga aatgacgctg tcgtttattc
    30661 cgaaaatcat cgccgtattt atcgccatca ttattgccgg accgtggatg ctcaatctgt
    30721 tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg tagcccgtac
    30781 aatgatgcag gagacaagcg atcaatggct ttcctggtta agcctgtact tctggccgtt
    30841 actgcgcgtg ctggcgctca tttctaccgc gccaattctg agcgaacgca gcgtgccgaa
    30901 acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc attgccccat cattacctgc
    30961 caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtacagc agatcctgat
    31021 cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggcga
    31081 aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt gtcgatcctg gcagccatct
    31141 caatatgccc gttttagcgc gcatcatgga tatgctggca ttactgctgt tcctgacatt
    31201 taacggtcat ttatggttga tttcactgct ggtcgatacc tttcacaccc tgccgattgg
    31261 tagcgaaccg ttgaacagca atgcgtttct ggcacccacc aaagcaggga gtttgatttt
    31321 ccttaacggg ctgatgctgg cgttaccgct cattactctg ttgctgaccc tgaatctggc
    31381 attaggttta ctgaatcgta tggccccgca attatccatt tttgttattg gatttccatt
    31441 aactctgact gtcggcatct ctttaatggc ggcattaatg ccgttaattg cgcctttttg
    31501 cgaacattta ttcagtgaaa tttttaattt gctggctgat attattagtg aattaccatt
    31561 aatataattc cgtaacgttt atcatgctac cctaaggatt atccgaaaaa taatatctgc
    31621 gaacatcttc caggatactc ctgtagcgaa atatttgttt taagctcact cacatatcgc
    31681 aacatttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga tagtaagtcc
    31741 gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg ttgttccgtt tagtcatgat
    31801 gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc catgtcaacg
    31861 attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct gttgagtaga
    31921 ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc catagcttgt
    31981 gattcacagc gtccttcagt ggtgtttatt aatgaggact gtttcatcca cgatgcttct
    32041 aacagtcagc gcatcaagca catcattaat caacatccca atacgttatt tatcgttttt
    32101 atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa tttattgatc
    32161 agttctaaat cgattaaacc ggaatctctc gacgatatcc ttggcgatat tctgaaaaaa
    32221 gagacaacga taacctcgtt tttaaatatg ccgacgttat cattgagccg aaccgaatcg
    32281 agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctccga ccaaatgaat
    32341 atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat caaaacgcat
    32401 aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa tggtattttt
    32461 gtcaacatgc gctaacacat tctgactggt ggtttcccac cagtcaggct gaataagatt
    32521 actctgcttt ctcaacaaag ataccgtcct gatgccctgc ttcattaaag aaccagatgc
    32581 cgagcgggta gtcttccagc gcaaccaggt acattgtgcc ttcactaaac tcctcaactg
    32641 ccagtaccac tccaggacga cgcggaccgc catccgtttt gactgttacc cgatcattca
    32701 ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt ttttctggca
    32761 gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag atgccaggcg
    32821 gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga tgaaaaccgc
    32881 aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc
    32941 ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt ctgaacacgt
    33001 tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct tcgaactgga
    33061 tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac
    33121 cctgagggtg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat tcgttacacc
    33181 aggaaatctg atgtgttcat caccttatct gcaatttttt tcgctgacaa gaaaatattc
    33241 gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg acgcgcgtca ccccttggtg
    33301 gctctggtgt caacgggatc gctcccgaaa aaatcatctt tgattatttg tgctgttttg
    33361 agaatcatcc tggtaagggg agtactgcgc gaccatgttt tcaattcaac aaccactact
    33421 ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact ggcaaccggc
    33481 agccccctgg ctcagccgtt tacacgaagc caatattccc gtcattctct gtagcagtaa
    33541 aactttagcg gaaatgctgt acttgcaaaa aatgttgggg ctacaaggct taccgctgat
    33601 tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag acggttttcc
    33661 acgcatcatc tcaggtatta gccatggcga aatctgccag gttttaaata cgctacgcga
    33721 gaaagaacat tttaaattca cgacttttga tgatgtcgac gatgcaacca ttgccgaatg
    33781 gacgggatta agccgtagct aggcggcgct gacgcagctg catgaggcgt cggtaacgct
    33841 aatctggcgc gacagtgacg agcgtatggc acaatttatc gctcgtctga acgaactggg
    33901 cttgcagttt atgcagggtg cgcgcttctg gcacgtactg gatgcctctg ccggaaaaga
    33961 tcaggctgcc aactggatta tcgcgaccta tcaacaattg tcaggcagac gcccaaccac
    34021 acttggcctg ggcgatgggc caaacgatgc gcctttactg gaggtaatgg attacgcggt
    34081 gattgtgaaa gggctaaacc gtgaaggggt acatctgcat gatgaggatc cggcccgcgt
    34141 ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa gggctggacc attttttctc
    34201 cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc ccggcctgtt ttgcgaggta
    34261 aagccgacgg tcggccaatg actggagttg ttcaaaatca taatcaccgg tttcctcgct
    34321 gctacttacc cccagcgagg cactgatgcg tatcgttgta ctcttggcga tcagcatctc
    34381 tttttcattt aacttcaggc gaatacgttc tgcgacctcc gcagcctgcg tcagattcgc
    34441 gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt cctgcgcacg
    34501 caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg cctgatgacc
    34561 aaagcggtca ttaatcgctt taaaatggtc aagatcgacc tgaatgacag aaaaaggatg
    34621 ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt tcgaacagtg cgccacggtt
    34681 atataaacgc gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt gcagaacata
    34741 catgttgctg accatccggc gaatcacata ccaggagagg agtaacatgg tggtaaagag
    34801 cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca cgccttcgct
    34861 tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc agacgttccc agctaacata
    34921 gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc attgcctgcg ccagcaaggc
    34981 cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag aggtcaaaaa
    35041 tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga tggcgtttct
    35101 taaaaattgc tgcatagtac gcacgggaat actcatcccc agcacgccat accagtagtt
    35161 attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg cgtgttccgg
    35221 ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct cgttgcgaat ggccgataaa
    35281 ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg
    35341 ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc gcctgttcaa ccatcgacga
    35401 ggagttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa tttcattgtc
    35461 gaggctttca ttttcgcgag acaggaaatt cccctcgctg actaatgcat ccgatacgcc
    35521 attgactggc agggtgcgcc gtcggttgag ttcgatttgc caggcgtgct cgtcgcgatg
    35581 ctgttcgaac tcggttacgg cattacgcag agaggtgaaa tccagtggcg ccacgagagc
    35641 ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca aatgcttatc
    35701 gagcgcgttg gctacgtttt ccagatgatt acgctagctg gagatatagg catcttccag
    35761 caccaccact tcacgccagg tgagcaaggt ggaaaaaagc aataccacga taaagcagag
    35821 attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc
    35881 catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg attctggcat
    35941 aacccggcaa tagcgtaccg caaaacagcg ataattttat aatggtggca tgataagacg
    36001 cgactatcat tcattgcagg cattgggaca ccaggccgga tgtgatccgg cctggtgtta
    36061 atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac aagatcggtt
    36121 tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc aaccataccg
    36181 ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac ttcctcacta
    36241 accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct tagttgaaga
    36301 tggcgaattt tgcataatag ccagtgcgag tattaatgtg cctgaccgcg cattttcgct
    36361 actgctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat cagatttaaa
    36421 acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgatatg ttcaatagcg
    36481 tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa catcgccagc
    36541 gtgccgacaa tcgataatgc tttcatcagc caaggcgcga taatcaataa ccctttacct
    36601 aatgcctgca tcagcgcgct agatttttcc gccagccaat accctaggtc atcaatctta
    36661 acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat gcctgaaagc
    36721 accagcacct gattaagcaa cggcgcctct gccacaatcc ccagcgtgat ggcgacgatt
    36781 tccgcagaca aaataaaatc ggtacgaatc gcccctttaa ttttgtcctt ttcaaacttc
    36841 agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgctgg atcttcttta
    36901 tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca gagaaacgcg
    36961 ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa acgcactgat gatcagcgcc
    37021 agcggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca gaccacggga
    37081 agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa atcatcccct
    37141 aatacaccgg cggttttctt cgccgccagt ttgcccatca cagagatatc gtccagcagt
    37201 gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat
    37261 ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac ttgcttcaca
    37321 ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct cgccagcaga atgattttca
    37381 cgtttactat gaaccacctt tttatttccg ccgtgaggga ttatgcgttt ccgccagttg
    37441 ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac ctattttgtc
    37501 attcggattg gcgtgggaag ctggcctccg ttaatgatgg cgggcgttcg attccaggca
    37561 gccggtattt tattgctggc atttttgcta ctgcgcggac acaaacttcc cccgctacgt
    37621 ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa tggcatggtg
    37681 acggttgccg aacatcaaaa tgttccttcc ggcatcgccg ccgtagtgat tgcaaccgtg
    37741 cccctcttta ccctgtgctt cagtcgcctg tttggcatta aaacgcgcaa actggaatgg
    37801 gtgggtattg ccattgggct tgccggaatc atcatgctca atagcggtgg aaatttaagc
    37861 ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc gtttggctca
    37921 gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat tgagatgctg
    37981 gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg gtgaaaaact gacggcgctc
    38041 ccttccctgt caggcttcct tgcggtcggc tatctggcgc tgtttggttc gattatcgcc
    38101 attaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac cagctacgcc
    38161 tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggtggcga aacgctgtcg
    38221 aagattgaat ggctggcgct cggcgtaatt gttttcgcgg tggtactggt cacgttggga
    38281 aaatatctct tcccggccaa acccgttgtc gcgccagtta ttcaggatgc atcaagcgag
    38341 taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga tccactcttc
    38401 cagacgctcg gtaagcgccg catccgtcag cttctcgcgc ctacgtaacg cacactccca
    38461 gacaatcagt acgcgccagc cgagctcctg caagcgactg atatcgcggc gatcgcgttc
    38521 aacattttta cctatcttct ccagccagaa ctcagttcga gtcgcaggca ctttaaacag
    38581 atagcagtga tgatgatgcc agaagcagcc gtgggtaaat atcacgcagc gatattcatc
    38641 aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg caaggccctg
    38701 cccggttaac aaactggcga ggcgcttctc aatcgccgta tcacgcgtgg caatcgcgcg
    38761 catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc atgttgtgcc tcttgctgac
    38821 gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg gcaaagaccg
    38881 gcacgaccac cgagttgccg aactggcgat aggcctgagt gtccgaaacc ggaatgcgga
    38941 atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcacga ggcgttaacc
    39001 gacgtggacg atgttgctga ttcagcggat cgtcaaagtc tttctcaccc gtggccatat
    39061 cccaaccgcg atcgattaaa atttccgcgc catctttgta ataacgagca gacagcgtac
    39121 gggtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg ttaccgcgcg
    39181 cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc aggatatatt
    39241 tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac tcgctgtgca gggaaacatt
    39301 cgctgatatc acgcagagta aaatcggctt tcagattcag atcgcgacga aaacccacca
    39361 gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc atcgatgatt ttcggatcgt
    39421 ccggcccgtt atcttctgca tcggcaacgt catagcccag ttcgtccagc gtctgcatga
    39481 tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt tttgacgttt tcgagcacaa
    39541 acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc gtgccctggg
    39601 tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg ccagccagcg
    39661 aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa
    39721 tatgttccgc cgccgcctca tcactcacgc cttcttgatg actgagggtg atgtcgcgga
    39781 tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg gttggcttta taagtgcgta
    39841 ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc accaatcgat tcaaaaccac
    39901 gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa tgcggatgat
    39961 gttccggtgg tttgggtaac aacgtttgca gatgggcaaa ctctttctca cttaaacggt
    40021 gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag tgattctcac
    40081 ccacaccatt aagctgcgcc accagcattt taacatcata aatttgcagc agtttctcca
    40141 gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata ttttcctgca
    40201 tagatttaac cggccatctg agatggccga caggttaaca taattactgc cgtttagggc
    40261 cacgcgctca gccagcgggt gaaaacgcat ctatcacctt gtgatcgata ccttcattct
    40321 cccccgccag ttcggcaccg agcttcgcca tcaaacagcg ccaagctcaa tgcccctgaa
    40381 acggcaaata cgcgcgccag gccaatcgcc cccagggctt ccagccgatc cgcatcctgg
    40441 acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg agcggcaatg
    40501 gcatgacaaa cggcctcaat tttctccgcc ggaaattgca cgaactcttc acggagcagg
    40561 cgacgcgtct cttctgccgc caggattgaa gaacgctgcc gttgcggatg atttttcgcc
    40621 aggctcacga tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc gacatcgtca
    40681 tctgccgcca gtttctgtgc cgtagcccag acacggcgaa aatgacacag gtcatgcgcc
    40741 gcgtcctgat gttgatgatg gtttttcaac cagttctcga actatgcctg ccagtgttgt
    40801 aactccatac gcgctccgtt gccaaagagt ctcaacctta gcagttttta ttcatagcgt
    40861 ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc cgcctaacaa ggcaccaacc
    40921 agggccataa acagaaatcc gaccaatgct cctaaccact tccccgcggt agatcctaaa
    40981 tcactttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca gatgtatcgc
    41041 gccattgccc agacgaaata gccacaccat gtatagaaag cccac
//
LOCUS       DBBBSB010000023        40895 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.023, whole genome shotgun sequence.
ACCESSION   DBBBSB010000023 DBBBSB010000000
VERSION     DBBBSB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40895
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.023"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACIUS0_001204"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="ACIUS0_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGB0219037.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="ACIUS0_001205"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="ACIUS0_001205"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGB0219038.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="ACIUS0_001206"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="ACIUS0_001206"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGB0219039.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="ACIUS0_001207"
                     /gene_synonym="trmU"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="ACIUS0_001207"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGB0219040.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="ACIUS0_001208"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="ACIUS0_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGB0219041.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="ACIUS0_001209"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="ACIUS0_001209"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGB0219042.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="ACIUS0_001210"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="ACIUS0_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGB0219043.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="ACIUS0_001211"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="ACIUS0_001211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGB0219044.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="ACIUS0_001212"
     CDS             complement(8942..9223)
                     /locus_tag="ACIUS0_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219045.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="ACIUS0_001213"
     CDS             complement(9237..10898)
                     /locus_tag="ACIUS0_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGB0219046.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="ACIUS0_001214"
     CDS             complement(10882..11208)
                     /locus_tag="ACIUS0_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGB0219047.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="ACIUS0_001215"
     CDS             complement(11264..11446)
                     /locus_tag="ACIUS0_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219048.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="ACIUS0_001216"
     CDS             complement(11430..11870)
                     /locus_tag="ACIUS0_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGB0219049.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="ACIUS0_001217"
     CDS             complement(11870..12166)
                     /locus_tag="ACIUS0_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGB0219050.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="ACIUS0_001218"
     CDS             complement(12163..12501)
                     /locus_tag="ACIUS0_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGB0219051.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="ACIUS0_001219"
     CDS             complement(12498..13709)
                     /locus_tag="ACIUS0_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGB0219052.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="ACIUS0_001220"
     CDS             complement(13711..14283)
                     /locus_tag="ACIUS0_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGB0219053.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="ACIUS0_001221"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="ACIUS0_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="ACIUS0_001222"
     CDS             complement(15781..16005)
                     /locus_tag="ACIUS0_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219054.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16046..16159)
                     /locus_tag="ACIUS0_001223"
     CDS             complement(16046..16159)
                     /locus_tag="ACIUS0_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGB0219055.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            complement(16234..16308)
                     /locus_tag="ACIUS0_001224"
                     /pseudo
     CDS             complement(16234..16308)
                     /locus_tag="ACIUS0_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16318..16623)
                     /locus_tag="ACIUS0_001225"
     CDS             complement(16318..16623)
                     /locus_tag="ACIUS0_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219056.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="ACIUS0_001226"
     CDS             complement(16620..16898)
                     /locus_tag="ACIUS0_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219057.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="ACIUS0_001227"
     CDS             complement(17187..18935)
                     /locus_tag="ACIUS0_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HGB0219058.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="ACIUS0_001228"
     CDS             complement(18932..19231)
                     /locus_tag="ACIUS0_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGB0219059.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="ACIUS0_001229"
     CDS             complement(19237..19470)
                     /locus_tag="ACIUS0_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219060.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="ACIUS0_001230"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="ACIUS0_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="ACIUS0_001231"
     CDS             complement(19685..19906)
                     /locus_tag="ACIUS0_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219061.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="ACIUS0_001232"
     CDS             complement(19896..20096)
                     /locus_tag="ACIUS0_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGB0219062.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="ACIUS0_001233"
     CDS             20226..20405
                     /locus_tag="ACIUS0_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219063.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="ACIUS0_001234"
     CDS             20530..20859
                     /locus_tag="ACIUS0_001234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219064.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="ACIUS0_001235"
     CDS             complement(20909..21097)
                     /locus_tag="ACIUS0_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0219065.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="ACIUS0_001236"
     CDS             complement(21472..22701)
                     /locus_tag="ACIUS0_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0219066.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="ACIUS0_001237"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="ACIUS0_001237"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGB0219067.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="ACIUS0_001238"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="ACIUS0_001238"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGB0219068.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="ACIUS0_001239"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="ACIUS0_001239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGB0219069.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="ACIUS0_001240"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="ACIUS0_001240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGB0219070.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="ACIUS0_001241"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="ACIUS0_001241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGB0219071.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="ACIUS0_001242"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="ACIUS0_001242"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGB0219072.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="ACIUS0_001243"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="ACIUS0_001243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGB0219073.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="ACIUS0_001244"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="ACIUS0_001244"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGB0219074.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="ACIUS0_001245"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="ACIUS0_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGB0219075.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="ACIUS0_001246"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="ACIUS0_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGB0219076.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="ACIUS0_001247"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="ACIUS0_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGB0219077.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="ACIUS0_001248"
     CDS             34739..35812
                     /locus_tag="ACIUS0_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGB0219078.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="ACIUS0_001249"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="ACIUS0_001249"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGB0219079.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="ACIUS0_001250"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="ACIUS0_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGB0219080.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="ACIUS0_001251"
     CDS             complement(40574..40786)
                     /locus_tag="ACIUS0_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HGB0219081.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9623 a        10793 c        10105 g        10374 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gtggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgat
//
LOCUS       DBBBSB010000024        40544 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.024, whole genome shotgun sequence.
ACCESSION   DBBBSB010000024 DBBBSB010000000
VERSION     DBBBSB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40544
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.024"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..956)
                     /locus_tag="ACIUS0_001252"
     CDS             complement(<1..956)
                     /locus_tag="ACIUS0_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGB0219082.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACIUS0_001253"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACIUS0_001253"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGB0219083.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACIUS0_001254"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACIUS0_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACIUS0_001255"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACIUS0_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGB0219084.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACIUS0_001256"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACIUS0_001256"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGB0219085.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACIUS0_001257"
     CDS             complement(5018..6346)
                     /locus_tag="ACIUS0_001257"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGB0219086.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="ACIUS0_001258"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="ACIUS0_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACIUS0_001259"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="ACIUS0_001259"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGB0219087.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACIUS0_001260"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="ACIUS0_001260"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGB0219088.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACIUS0_001261"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="ACIUS0_001261"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGB0219089.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACIUS0_001262"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="ACIUS0_001262"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGB0219090.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACIUS0_001263"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="ACIUS0_001263"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGB0219091.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="ACIUS0_001264"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="ACIUS0_001264"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGB0219092.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="ACIUS0_001265"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="ACIUS0_001265"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGB0219093.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACIUS0_001266"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="ACIUS0_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGB0219094.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="ACIUS0_001267"
     CDS             18267..19166
                     /locus_tag="ACIUS0_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGB0219095.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="ACIUS0_001268"
     CDS             19172..21604
                     /locus_tag="ACIUS0_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGB0219096.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="ACIUS0_001269"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="ACIUS0_001269"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGB0219097.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="ACIUS0_001270"
     CDS             22717..23982
                     /locus_tag="ACIUS0_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGB0219098.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="ACIUS0_001271"
     CDS             complement(24223..25815)
                     /locus_tag="ACIUS0_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGB0219099.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="ACIUS0_001272"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="ACIUS0_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGB0219100.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="ACIUS0_001273"
     CDS             complement(27012..28130)
                     /locus_tag="ACIUS0_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGB0219101.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACIUS0_001274"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="ACIUS0_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGB0219102.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="ACIUS0_001275"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="ACIUS0_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGB0219103.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="ACIUS0_001276"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="ACIUS0_001276"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGB0219104.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACIUS0_001277"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="ACIUS0_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGB0219105.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="ACIUS0_001278"
     CDS             complement(31379..31444)
                     /locus_tag="ACIUS0_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219106.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="ACIUS0_001279"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="ACIUS0_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGB0219107.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="ACIUS0_001280"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="ACIUS0_001280"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGB0219108.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="ACIUS0_001281"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="ACIUS0_001281"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGB0219109.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKVNIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="ACIUS0_001282"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="ACIUS0_001282"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGB0219110.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="ACIUS0_001283"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="ACIUS0_001283"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGB0219111.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="ACIUS0_001284"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="ACIUS0_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACIUS0_001285"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="ACIUS0_001285"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGB0219112.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /gene="mcbA"
                     /locus_tag="ACIUS0_001286"
     CDS             39719..39979
                     /gene="mcbA"
                     /locus_tag="ACIUS0_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGB0219113.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40544
                     /locus_tag="ACIUS0_001287"
     CDS             40244..>40544
                     /locus_tag="ACIUS0_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGB0219114.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10098 a        10717 c         9865 g         9864 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcagaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagtaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgaa ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc ggcg
//
LOCUS       DBBBSB010000025        38250 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.025, whole genome shotgun sequence.
ACCESSION   DBBBSB010000025 DBBBSB010000000
VERSION     DBBBSB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.025"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..74)
                     /locus_tag="ACIUS0_001288"
     CDS             complement(<1..74)
                     /locus_tag="ACIUS0_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HGB0219115.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACIUS0_001289"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACIUS0_001289"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGB0219116.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACIUS0_001290"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACIUS0_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGB0219117.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACIUS0_001291"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACIUS0_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGB0219118.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACIUS0_001292"
     CDS             2704..2940
                     /locus_tag="ACIUS0_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGB0219119.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACIUS0_001293"
     CDS             2943..3800
                     /locus_tag="ACIUS0_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGB0219120.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACIUS0_001294"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACIUS0_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACIUS0_001295"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACIUS0_001295"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGB0219121.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACIUS0_001296"
     CDS             complement(6415..7311)
                     /locus_tag="ACIUS0_001296"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGB0219122.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACIUS0_001297"
     CDS             complement(7360..7599)
                     /locus_tag="ACIUS0_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGB0219123.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACIUS0_001298"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACIUS0_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGB0219124.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACIUS0_001299"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACIUS0_001299"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGB0219125.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACIUS0_001300"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACIUS0_001300"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGB0219126.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACIUS0_001301"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACIUS0_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGB0219127.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACIUS0_001302"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACIUS0_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..16476)
                     /locus_tag="ACIUS0_001303"
     CDS             complement(15268..16476)
                     /locus_tag="ACIUS0_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGB0219128.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16499..16909
                     /gene="tnpA"
                     /locus_tag="ACIUS0_001304"
                     /pseudo
     CDS             16499..16909
                     /gene="tnpA"
                     /locus_tag="ACIUS0_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACIUS0_001305"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="ACIUS0_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGB0219129.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="ACIUS0_001306"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="ACIUS0_001306"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGB0219130.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="ACIUS0_001307"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="ACIUS0_001307"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGB0219131.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="ACIUS0_001308"
     CDS             complement(19387..20556)
                     /locus_tag="ACIUS0_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219132.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACIUS0_001309"
                     /gene_synonym="ynhF"
     CDS             complement(20722..20811)
                     /gene="cydH"
                     /locus_tag="ACIUS0_001309"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGB0219133.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            21110..22135
                     /gene="purR"
                     /locus_tag="ACIUS0_001310"
     CDS             21110..22135
                     /gene="purR"
                     /locus_tag="ACIUS0_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGB0219134.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACIUS0_001311"
     CDS             complement(22132..23064)
                     /gene="punR"
                     /locus_tag="ACIUS0_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGB0219135.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23177..24388
                     /gene="punC"
                     /locus_tag="ACIUS0_001312"
     CDS             23177..24388
                     /gene="punC"
                     /locus_tag="ACIUS0_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGB0219136.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24679..25827
                     /gene="cfa"
                     /locus_tag="ACIUS0_001313"
     CDS             24679..25827
                     /gene="cfa"
                     /locus_tag="ACIUS0_001313"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGB0219137.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACIUS0_001314"
     CDS             complement(25867..26508)
                     /gene="ribE"
                     /locus_tag="ACIUS0_001314"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGB0219138.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26723..28096
                     /gene="mdtK"
                     /locus_tag="ACIUS0_001315"
     CDS             26723..28096
                     /gene="mdtK"
                     /locus_tag="ACIUS0_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGB0219139.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28137..29393)
                     /locus_tag="ACIUS0_001316"
     CDS             complement(28137..29393)
                     /locus_tag="ACIUS0_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGB0219140.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDCA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29701..29777
                     /locus_tag="ACIUS0_001317"
     tRNA            29701..29777
                     /locus_tag="ACIUS0_001317"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29735..29737,aa:Val,seq:gac)
     gene            29782..29858
                     /locus_tag="ACIUS0_001318"
     tRNA            29782..29858
                     /locus_tag="ACIUS0_001318"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29816..29818,aa:Val,seq:gac)
     gene            29966..30271
                     /locus_tag="ACIUS0_001319"
     CDS             29966..30271
                     /locus_tag="ACIUS0_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGB0219141.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30397..31819
                     /locus_tag="ACIUS0_001320"
                     /pseudo
     CDS             30397..31819
                     /locus_tag="ACIUS0_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACIUS0_001321"
     CDS             complement(31831..32643)
                     /gene="ydhT"
                     /locus_tag="ACIUS0_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGB0219142.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACIUS0_001322"
     CDS             complement(32647..33432)
                     /gene="phsC"
                     /locus_tag="ACIUS0_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGB0219143.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33429..34097)
                     /locus_tag="ACIUS0_001323"
     CDS             complement(33429..34097)
                     /locus_tag="ACIUS0_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGB0219144.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34161..34799)
                     /locus_tag="ACIUS0_001324"
     CDS             complement(34161..34799)
                     /locus_tag="ACIUS0_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGB0219145.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34812..36913)
                     /locus_tag="ACIUS0_001325"
                     /pseudo
     CDS             complement(34812..36913)
                     /locus_tag="ACIUS0_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36934..37560)
                     /locus_tag="ACIUS0_001326"
     CDS             complement(36934..37560)
                     /locus_tag="ACIUS0_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGB0219146.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38016..38225)
                     /gene="fumD"
                     /locus_tag="ACIUS0_001327"
     CDS             complement(38016..38225)
                     /gene="fumD"
                     /locus_tag="ACIUS0_001327"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGB0219147.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9209 a         9992 c         9366 g         9683 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt ttacacactg tgatgaaaaa
    20881 atctcccgtc atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc
    20941 caaaattcca cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg
    21001 tcgtttttcc ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt
    21061 taccttgcga ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat
    21121 aaaagatgta gcgaaacgag caaacgtttc cactacaact gtgtcacacg tgatcaacaa
    21181 aacacgtttc gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca
    21241 ctactcccct agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt
    21301 gctggcgacc agcagcgaag cggcctattt tgccgagatc attgaagcag ttgaaaaaaa
    21361 ttgcttccag aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca
    21421 gcgggcttat ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc
    21481 tgagtaccca gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt
    21541 gatggactgg ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga
    21601 aggcggctac atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat
    21661 ccccggcccg ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat
    21721 ggaagaagcg atgatcaagg tgccggaaag ctggattgtg cagggtgact ttgaacctga
    21781 atccggttat cgcgccatgc agcaaatcct gtcgcagccg catcgcccta ctgccgtctt
    21841 ctgtggtggc gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggcctgcg
    21901 cgtcccgcag gatgtttcgc tgatcggtta tgataacgtg cgcaacgcgc gctattttac
    21961 gccggcgctg accacgatcc atcagccaaa agattcgctg ggtgaaacag cgttcaacat
    22021 gctgttggat cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg
    22081 tttgattgaa cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc
    22141 cgttgcggga gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgct
    22201 gagatactca agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca
    22261 acatgccgaa tccgggaagg ggttttccag ttcaagtgct acccacttcc cctcattaag
    22321 ccacggtttg gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt
    22381 tgccgatgat tcccagtcag gtacgacaag tcttttttgg ttatccagca accaggtaat
    22441 acgtttaggt agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt
    22501 atcatcgctg aacgggccat ccatcaacgc cagcgggtgg tgactggcaa caacgcaact
    22561 ccagcttagc atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt
    22621 tgcgccaatt gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac
    22681 ttcctgaaag acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg
    22741 ccgtgtacgt tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc
    22801 gttcgctatc tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc
    22861 ttctttgaga aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa
    22921 gagcggtacc gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac
    22981 acgatgcagc tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac
    23041 ttcgagtgaa tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa
    23101 tattaccgtt tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga
    23161 gatttagaga atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag
    23221 cgtactcggt tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga
    23281 cctgcaaacg cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc
    23341 cgcagcccag cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt
    23401 aatcggcctg acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc
    23461 cacgctgctg gtattgcgtt ttgtacaggc tgtgggggtc tgcgccgcgg cggttatctg
    23521 gcaagcatta gtgacagatt attatccttc acagaaagtc aaccgtattt ttgcgaccat
    23581 catgccgctg gtgggtctgt ctccggcact ggctcccctg ttaggaagct ggctgctggt
    23641 ccatttttcc tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct
    23701 gcctattttc tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac
    23761 ctttaccgac ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcagcctg
    23821 ttcagccagt ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta
    23881 cagcccggca gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg
    23941 ttatggctgt cgcgccgcac tgcagaaatg gcaaggcaag cagttattac cgtggttgct
    24001 ggtgctgttt gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc
    24061 gctggtcgaa atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc
    24121 tattgttgtc gcccaggcgc tgcgtccctt cccacatgca accggtcgcg ccgcagcgtt
    24181 gcagaacact ctgcaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat
    24241 tagtatcagc acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt
    24301 cgcgctgggt tacatgatgc aacgttgtga agaagttgac tgcccgaatc atggcaatgc
    24361 cgaagtcgct catagcgaat cacactgatc tatatcgata tacttatact taggctgcta
    24421 acaaaatttt gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct
    24481 acgataaata ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat
    24541 ttcggcaagg gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt
    24601 ctgcgagatt tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg
    24661 accagtgatg gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac
    24721 aactggtacc gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct
    24781 gccccggcgg atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaggaaggc
    24841 tctttggggt taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg
    24901 ttttttagca aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac
    24961 acgctgcgta ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata
    25021 gtcggcaagg agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc
    25081 atgcaatatt cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg
    25141 aagctcaaaa tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt
    25201 ggctgcggct ggggcggact agcgcactac atggcatcta actatgacgt aagcgtggtc
    25261 ggcgtcacca tttctgccga acagcaaaaa atggctcagg aacgctgtaa aggcctggat
    25321 gtcaccattt tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct
    25381 gtggggatgt tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat
    25441 cgtaatttga aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat
    25501 ctgaatgttg atccctggat taataaatat atttttccga acggttgcct gccctctgta
    25561 cgccagattg ctcaatccag cgaaccccac tttgtgatgg aagactggca taacttcggt
    25621 gctgattacg atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa
    25681 attgcggata actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc
    25741 gcaggtgctt tccgcgcccg tgatattcag ctctggcagg tggtgttctc acgcggtgtt
    25801 gaaaacggcc ttcgagtcgc tcgctaaagg ctattctatc gccccctctt cgggggcgag
    25861 ttcagatcag gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg
    25921 ttctaccgta tctaccactg cctgagtttg tggatcgatt tcaatgttga cgcgtgcgcc
    25981 aagttttttc ttcccaagag tcgtgcgttc cagtgtttcc ggaattaaat ggacgcaaaa
    26041 acgcgttggc gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt
    26101 gtacaggata tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt
    26161 ttccgaggtt aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg
    26221 tccgccaatt tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt
    26281 taaatcgcca agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt
    26341 gccgttaatt tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc
    26401 caggccgtcc agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc
    26461 gtcaatcgac accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc
    26521 tgaaatcagt taagacattc tgttcagcac aatagcaggt ggaaaacgcc cttaccagtg
    26581 aaggggtaag aatggctatt ttttcactgg agaattaata aatcctcgct acaatagact
    26641 gaatttcccc tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata
    26701 actacaaata aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc
    26761 attagcaatc ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac
    26821 cgtgatggcg ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat
    26881 ctggcttccg gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc
    26941 gcaattaaat ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg
    27001 gctggcaggt tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat
    27061 ccgctacatg gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc
    27121 gttgttgtgg ggcgcgccgg gatatctgtt cttccaggtt gcccgtaacc aatgtgaagg
    27181 tctggcaaaa accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacatccc
    27241 ggtgaactat atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg
    27301 tggcgtggct actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa
    27361 acgcgcccgc tccatgcgcg atattcgtaa cgaaaaaggc accgctaagc ccgagcctgc
    27421 ggttatgaaa cgactgattc aactcggttt gccgattgcg ctggcactgt tctttgaagt
    27481 gacactgttt gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg
    27541 acaccagatt gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc
    27601 agcggtaact atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac
    27661 cgctgcgcgg accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac
    27721 ggtttcactg cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct
    27781 ggctgcgcat ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat
    27841 tggcagtggg attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac
    27901 agcttactgg gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt
    27961 tgaacctatg gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc
    28021 cattatgatg atgctgcgta tgcggttcct gcaacgtatg ccgtcagcca tcattctgca
    28081 acgagcgtcc cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac
    28141 gcaggctgga gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttacac
    28201 agagtttgcc gactggcatc tgttatctca acgcgtatat ccccgttaac ttcccctttc
    28261 acattgatga agtcaggtga aatttctttc gcactggttt gcaggcaaat cgctccctct
    28321 ccgcgcaggc gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt
    28381 tttccgccat tcagattgat gttaccaaca atgaaagtga cgctgtcacc ttttaccgta
    28441 ctgccgtcga gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagctct
    28501 gcgccataac caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca
    28561 accggtgcgc aatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg
    28621 atcactgttc caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccgc
    28681 ccccccacat tcaccgacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc
    28741 gtccccagac gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga
    28801 ttattcatgg tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg
    28861 ccgccctgat taatctcaca attgctggct tctccacttg gatacaccaa caattcacca
    28921 ccttcatcta ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg
    28981 agaatgattt tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc
    29041 aacaaaccgc aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta
    29101 accgtagcga gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca
    29161 atggcaccgt cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc
    29221 ccaccggttg ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg
    29281 ccagcttcaa caatcaactc gccttcagta agttgcgtcg cgccaaggac ctcgccggtc
    29341 ttaacaattt gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat
    29401 tcctttgctg catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta
    29461 tccacgacag atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat
    29521 tgggttgtat cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa
    29581 tatagccaat ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt
    29641 gcctcttgcc acctttccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt
    29701 gcgttcatag ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag
    29761 tccaattgaa cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat
    29821 ggtgggggtc ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtctttt
    29881 cctttttccc tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt
    29941 atgcgttatc agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa
    30001 tggcccattt ggcgacgcaa tggctgagca gcttaaacca cttgctgagt cgattaatca
    30061 ggaacctggt tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg
    30121 gatctacctt ttcactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg
    30181 actgaaaaat ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact
    30241 tagtcaaatc aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc
    30301 ttttcatccc tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa
    30361 cactaaattc gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt
    30421 gccgggccta cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct
    30481 atttctatct tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa
    30541 aactcaaaaa tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg
    30601 tatctcgaat ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa
    30661 gaaaccttgc acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat
    30721 caatttttac gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa
    30781 tcatgccagg ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag
    30841 gatttaccca gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat
    30901 gaagaagaag caaccagaac gtattttccc agcccgtggt caggcctgat ggaagcaaag
    30961 gtcgatgcgt gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg
    31021 gcagtggcta ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttcac
    31081 cgcgataacg caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc
    31141 gaagccgatt tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca
    31201 ttaaatgctg agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata
    31261 gtagaggaaa tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg
    31321 aatgtcgatt cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac
    31381 tgggcagaaa aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc
    31441 tggcgttatg tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat
    31501 gaacacgacc ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg
    31561 gcaatccctt cagagtctat tcgtcgacta ctggccttac gtgaagcggg gatctgaaat
    31621 ccctgatgtt ggcgaagatt atacgttaca gcaacccgaa gatattcgtg gacgcgtagc
    31681 gttcggcgcg ttgccctggt tgatgcacga ccagcctttc gttcagggac ttacggcatg
    31741 tgcagaaatt ggtgaggcga tggctcgggc ggtcgtaaag cctgcatccc gtgctcgtcg
    31801 gcgtctttcg tttgattaaa ggtaaagcta tcagcactca acaaactgat gctgttcatt
    31861 aagacgatag cggactccgg ttcgaatatt gttttcacct tcaatggcga cggcgaagcg
    31921 aactctttcg ccgtcatgat atgccagtgc ggcgcttccc cctagcccga gaataattga
    31981 gtcgaccacg cccgtgctca ctaccgttga attttcaccg ctggcaatga tgtgtacatt
    32041 gtcgccgctg ttggcaattc tggcgtttgc gccaaagctg gcgatttgca ccaggtcacc
    32101 attactggcg acatgacacc gttcacccaa tgtacaaacg cgtactcgca ttccggtatt
    32161 cgcaatccga ctactatctc cagcgctgct tatccgcgaa gaattaccgg ttacagcaac
    32221 ccgtgcgcgt tcaccggagc tgccaatgtg actgttaaag ccgacacttc caatccgcac
    32281 gctataaccc atactggcaa tttgcgcggc atacccagcg gttgcgattc gggcgttatc
    32341 ctcacaacag ccgatgcgag catgatcgtc gctgcgggca aactgactgc aaaatggtat
    32401 ctctccgggg tctgtggcca gtttttgcat agaggaaact tcctgatgcg cgaagttttc
    32461 atccgccagc cattttttcc acgcatactc aaccagactc tccgcccagt ccgtatatcc
    32521 ttcctcaatc agtgcatgac gaatatcggc ataactaccg ccgcgtggga aatggcgcag
    32581 gaaccagcga tacatcaccg ctccgatacg ccattctcgc aggtctgctc gtgtaatgat
    32641 cattcgttag tgccggtgat agccatcgac catgctttta aaggtttcgt gtggcgtacg
    32701 ccccgtggtg caaagataaa gatgaccgaa gataaaaaag aggcttataa atgccagagc
    32761 aaaatgcgcc tgcaataacc agtatcttac gccaggaaac acatctccca cggcttgcgg
    32821 atagagacac aacagccccg tcaatagtaa caacggcagc aatccataca tgacaccaac
    32881 ataggcgacc tgctgtaagg gattaaattt agactgtgtt gttgccggga aaggatgttc
    32941 ttccccctgc ataatgccaa acaaataaaa tcgcgtttgt tttgccgctc gttccagcca
    33001 cccctgacga cgaatgcgat agtggtgacc attatcccca acggcattga tcagcacaaa
    33061 gccgagccag catgccagta acaaaaatcc gcaaacttca tgcaccgcaa ccagactttt
    33121 aaccgcagtt gcgcccacca tcgcaaagtg atttatcagc ccacttgcca gcaacaatac
    33181 aaagagtaac gcattcgacc agtgccataa tctgaccgct ttgctgtaaa gataaacctt
    33241 ctcaccatga tcggtggctg atttcttcac gccacgagca cgaagcaagg cgtgcagccc
    33301 cagcaccaac cacatcccaa ccagcaatac tccggcaatg atcaaccaca ccggccaaaa
    33361 ttcgggagtg aactgtggca catagttcgc taactggctc tgaaactgtt cagcatgttg
    33421 cgacgggttc atacattttc ctttttaatc aaatgttgac cgaaccggcg atacagatgc
    33481 ggttttccgg caccaggtag ctgatattga tagtatttat tatcctgcaa ccacgcctga
    33541 atttctgggc tatcttcgcg accaaagatc aatgcgtgtt ccggacaggc gctgacgcaa
    33601 atgggcggaa agccttttgc cagtcgggac tcagcgcaga aatcacattt atctgccact
    33661 ttagtgacag ggttaaggta gcgaaccagg tacggacaag cgccaatgca ataactgcag
    33721 ccgatgcact gcgatttttc tacccgcacg atcccctgct catcacgcca cgatgccccc
    33781 gtcgggcata cgtcgatgca cggagcatct tcgcagtgct gacatgactg gcgaaaaaag
    33841 tgatactgcg tttcgttgtc gttatcggtg actggaatgt gtgctatcga taaacggctt
    33901 ccctgagcgg ggacgtggtt agttttacgg catgcgcggg cgcaaatatt acagccattg
    33961 caccgtgact cgtcatgaat catggcatac cgaacttcct tttcttgcct cgtgttcgct
    34021 aacagagaag acgcagaacc agtaaaaaag attactgttc ccatccccag aacaaatttg
    34081 cgtcgagtga aggacatctg attatcccct aatgcagtgg ctgcaactgt ccaccctatc
    34141 catgacatct ccctgaaatt tcaggggttc tgaaactgct ctacctcgaa ccattcgcat
    34201 aagccataag ccttgccata ggtcataaat cgctcgctaa acaagtacaa caccgccgtc
    34261 gatgactcca cctgccgtat atcggcatat tcaggtgcaa catccatcgc ggcgatcgcg
    34321 gcttcaatct gcgcttcctg gaagtaatat ggcgcttgca tcagcatggc gactttgtaa
    34381 ggacgaggat acgtctggca ctcaaaacga acggcgtgtg caatggcgcg acaaatgtcc
    34441 tgctccacaa cttgcaaaga catcgccgcg tagttttcac tcatggcctg cgttgagtaa
    34501 tagtaatcat cctgacttcc cgtcatacaa gcgatatccg cgtagtctcc gttctgctta
    34561 atgctttcca gcaaaactga cagttcctct tctgcaaccg aatagggtgg cgagagaaac
    34621 acggtgcgcg ccaccagctg cccagccgca gaatgctggc gaatgaactc agccaggatt
    34681 tcagcaggtt ctggttcggt taccgtatca acggggtgac gcatcccctg caaccattgc
    34741 cgcttcgcct cgtcaacttc gctgactttc gctaacggca attcatcctg atggttcatc
    34801 ttacccattc cttacgcagg cagtagattg tgtgctgcca gatcggcggc aatatcttcc
    34861 agccccagac gctgcaatgt ttcgcgggtt ggacaaccaa gctgtgggtc ccagcccatt
    34921 tctttgtaga acatcgtgag cgaagcatgc atatcgtcac gatccatttt gtcagtacct
    34981 tcagtaaaga ccgggatctg cggatccttg tcgaaaaccc aggaacagat aagatcgtgt
    35041 tcattacgca tatctttggt ttgcatcggt tttaccgtgt aggcacgatg caacgtaaag
    35101 atacgctctg cagctaaatc taatttttcc tgagtcatct cttcgccggt gatcgctttg
    35161 aagaatttgg cttcaagcgc cagatcgccc cggtaattac ggcttttcag cggcgaaacg
    35221 gtcattggcc agacccagtt gcacagagtg acggcgttat gcaaacagac ccgcaacagc
    35281 gaccactttg catatttaat ttttgcgtca ttgattggcg tgtagttttt ggtttcatcg
    35341 taagcatctt cagaaccaaa aagttctttc gccacttcac gttgcagttt caatggcaag
    35401 ccggaaccaa taaagttgat atgggtatgc gtcatgcagt cacggttgaa catacactta
    35461 acaatggaac cgacctgcgc tgacgcttca ttggcatggt gaaccggata gccaaacggc
    35521 gaccagagtt tatttttcgc gtagccccag tactctttac ccaaattcca gcgttctgcg
    35581 atggcatatg aaccatcagc caggtgactc agctcaccca cacgatgcgc cagacggtag
    35641 taaaaatctt taatgaagtt aacgtcaccc gcttccagtt gatcccagcg aatttctgca
    35701 tactcttcag ctggcagaac acgcttgaac acacctttgc tgtaacaata agtaaagtcg
    35761 cgatgcaact gcccgtagtt acaccatagg ccgtagtcat cgaacaaatt cagaccaacc
    35821 aggttaccaa tcacacgacc atcgtcttta tcttcaaaat ctttcggccc gttcgggaag
    35881 atggtggtat ggacaaagtt tgctacacaa gtgttaccac ctgtgctggg cacgccaaac
    35941 tctttcaccc gaggaatatt catttgggtc atacaacgga tcgggcaaga gtggcagccg
    36001 ctcattttta ctgtgtattt ctctgccgcc ggtcctaagt caaaaacgga tttataggta
    36061 cgaaagccga ccgtattctg attgcctggc ggaatttcac ccgtttcaat cggaccacct
    36121 tcagccgcgc cccaaaacag ccctttacgt gctgtccagc gtgacttggg atctgaatac
    36181 tctgcccacg attgtggcgt acttggcacg acatggttgt tattcgcacc aataagttca
    36241 gtcatcatgt aatcattgag acgcttcatc tcctgacgat cggcaatgtt gacccctttc
    36301 gtcccttcaa ccgcaatcgc tttcaggttt ttcgaaccca ttattgcgcc agttcccgca
    36361 ccgccgctgt ggttacggct attcaacatg ccagagagag gaataaggtt ttccccagcc
    36421 tgaccaatag ccgccacaca ggtttccggg ctggtcaatc gacaaatttc ttccgtcgtt
    36481 gcgcgcgtcc cttttcccca taagaaatcg gctttttcca ggctaacttt gtcatcttta
    36541 atcttcagcc ataccggtga tttcgccttc ccttcgataa taatgacgtc gtatccagcg
    36601 aatttcattt gcgctgcaaa aaagccaccc atatgggcat cgcgactaaa tttcctttgg
    36661 taaaagtaga aagtgaggtg atatttacgc gagaactaca gggggcacca gatccaggta
    36721 atgggccggt agcaaagact aatttattcg cttcatcgaa aggtttcgtg cctagcggta
    36781 cttcgtcata cataattttg tagccgaagc ccatgccacc gacaaaactt ttaaacttac
    36841 tggaatcttc gagggtaata tttcctgtcg tgagattgac tcttaatata ttacctgtcc
    36901 aaccgttagc catgattttt tcctttgcaa gattcacaca gtaatatctt tccactcgat
    36961 aatttttaac gcccctgttg ggcaggcgtt tgcgcattca ccgcataaca cacatttcga
    37021 ggatttttta ctttcggtat ttacggtggc catcatccac gggcatgccg tagtacaggc
    37081 gctacagcca atacaacgtt tatgatcgac ggtaatacag ccttctttct gctgccaggt
    37141 aatcgcacca atcgggcaga cgttcatgca ttgcggctct ttgcattgac ggcaggtgtc
    37201 cgcggtatag ttgagatcgc catacaggcc gccgccagag ccaaccccgt tgtcgccaaa
    37261 gaaataattg cgatggattt tgatacggga gaagaatgtc cctactgagc catcgttgaa
    37321 gttggtacaa gagatttcac agcggtggca acccgtgcat cgcgctcgct gggtcaccag
    37381 cacccctttg ggtgtattta tcaaccctac tgtgccgcta tcaatatctt cctgtttgca
    37441 acccaaaagt gacagtaacg caggggcaat tgttagacca gcaaggcctt ttcctgatat
    37501 acgcaagaat tctaatcgcg ttaagccaat atctaatagt ggacgatcaa ccgggttcat
    37561 ttatttcgca tcctcttcgc aaacacagtg ctgacaattc agcagcctga tattaagcat
    37621 tcaactaata cgctgctgac tctgtcccca acaccagata ttttccctaa cccgatagtg
    37681 tatgagaaaa gatttaacgc cgataaacta atcctttatg gttaggagaa aaaataatgt
    37741 gattattctg agcccttaac attgatcgtt atcaattaaa attacaaagc aagaaattat
    37801 aagcgaactg atatttatta tcatttgaaa taaatttaac ttaagcgaca atcatttgtg
    37861 acgttcattg cagaaaataa aaataacatt atcgctataa attaaaatac ataccgaaat
    37921 tcgtataatt tcagcagttg ttatttagaa tgattaactg gaccgcaact gaagcaagaa
    37981 tatcaggatg aagcttaccg cctcatcctg aatggtcatt ttaccagtgc gttaatgacg
    38041 gtttccatcg cctgcttttc cagctcactg cgctgaatgc gtttattggc taatgctgta
    38101 cgtaatacgc ctgcgataac aatatgcttt ggttcctgcc ccagatcgcg catttccagc
    38161 acaactttac cgacaactct gcacatttct cggtacagtt catcctcttt tgtccgattt
    38221 cccattcttt atacctattt atcatgccaa
//
LOCUS       DBBBSB010000026        36722 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.026, whole genome shotgun sequence.
ACCESSION   DBBBSB010000026 DBBBSB010000000
VERSION     DBBBSB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36722
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.026"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            63..419
                     /locus_tag="ACIUS0_001328"
     CDS             63..419
                     /locus_tag="ACIUS0_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGB0219148.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            423..1550
                     /gene="dapE"
                     /locus_tag="ACIUS0_001329"
     CDS             423..1550
                     /gene="dapE"
                     /locus_tag="ACIUS0_001329"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGB0219149.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1578..1778
                     /locus_tag="ACIUS0_001330"
     CDS             1578..1778
                     /locus_tag="ACIUS0_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGB0219150.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1888..2586)
                     /gene="ypfH"
                     /locus_tag="ACIUS0_001331"
     CDS             complement(1888..2586)
                     /gene="ypfH"
                     /locus_tag="ACIUS0_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGB0219151.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2660..4675)
                     /gene="tmcA"
                     /locus_tag="ACIUS0_001332"
     CDS             complement(2660..4675)
                     /gene="tmcA"
                     /locus_tag="ACIUS0_001332"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGB0219152.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4690..5553)
                     /locus_tag="ACIUS0_001333"
     CDS             complement(4690..5553)
                     /locus_tag="ACIUS0_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGB0219153.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5721..6434)
                     /gene="purC"
                     /locus_tag="ACIUS0_001334"
     CDS             complement(5721..6434)
                     /gene="purC"
                     /locus_tag="ACIUS0_001334"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGB0219154.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6647..7681)
                     /gene="bamC"
                     /locus_tag="ACIUS0_001335"
     CDS             complement(6647..7681)
                     /gene="bamC"
                     /locus_tag="ACIUS0_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGB0219155.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7698..8576)
                     /gene="dapA"
                     /locus_tag="ACIUS0_001336"
     CDS             complement(7698..8576)
                     /gene="dapA"
                     /locus_tag="ACIUS0_001336"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGB0219156.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8722..9294
                     /locus_tag="ACIUS0_001337"
     CDS             8722..9294
                     /locus_tag="ACIUS0_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGB0219157.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9294..9764
                     /gene="bcp"
                     /locus_tag="ACIUS0_001338"
     CDS             9294..9764
                     /gene="bcp"
                     /locus_tag="ACIUS0_001338"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGB0219158.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10017..10634
                     /gene="hyfA"
                     /locus_tag="ACIUS0_001339"
     CDS             10017..10634
                     /gene="hyfA"
                     /locus_tag="ACIUS0_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGB0219159.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10634..12651
                     /gene="hyfB"
                     /locus_tag="ACIUS0_001340"
                     /pseudo
     CDS             10634..12651
                     /gene="hyfB"
                     /locus_tag="ACIUS0_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12662..13609
                     /gene="hyfC"
                     /locus_tag="ACIUS0_001341"
     CDS             12662..13609
                     /gene="hyfC"
                     /locus_tag="ACIUS0_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGB0219160.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13626..15065
                     /gene="hyfD"
                     /locus_tag="ACIUS0_001342"
     CDS             13626..15065
                     /gene="hyfD"
                     /locus_tag="ACIUS0_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HGB0219161.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15077..15727
                     /gene="hyfE"
                     /locus_tag="ACIUS0_001343"
     CDS             15077..15727
                     /gene="hyfE"
                     /locus_tag="ACIUS0_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGB0219162.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15732..17318
                     /gene="hyfF"
                     /locus_tag="ACIUS0_001344"
     CDS             15732..17318
                     /gene="hyfF"
                     /locus_tag="ACIUS0_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGB0219163.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            17308..19022
                     /gene="hyfG"
                     /locus_tag="ACIUS0_001345"
                     /pseudo
     CDS             17308..19022
                     /gene="hyfG"
                     /locus_tag="ACIUS0_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19032..19577
                     /gene="hyfH"
                     /locus_tag="ACIUS0_001346"
     CDS             19032..19577
                     /gene="hyfH"
                     /locus_tag="ACIUS0_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGB0219164.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19574..20332
                     /gene="hyfI"
                     /locus_tag="ACIUS0_001347"
     CDS             19574..20332
                     /gene="hyfI"
                     /locus_tag="ACIUS0_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGB0219165.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20325..20738
                     /gene="hyfJ"
                     /locus_tag="ACIUS0_001348"
     CDS             20325..20738
                     /gene="hyfJ"
                     /locus_tag="ACIUS0_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGB0219166.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20768..22780
                     /gene="hyfR"
                     /locus_tag="ACIUS0_001349"
                     /pseudo
     CDS             20768..22780
                     /gene="hyfR"
                     /locus_tag="ACIUS0_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22802..23650
                     /gene="focB"
                     /locus_tag="ACIUS0_001350"
                     /pseudo
     CDS             22802..23650
                     /gene="focB"
                     /locus_tag="ACIUS0_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23744..24805)
                     /locus_tag="ACIUS0_001351"
     CDS             complement(23744..24805)
                     /locus_tag="ACIUS0_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGB0219167.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25018..26481
                     /gene="bepA"
                     /locus_tag="ACIUS0_001352"
     CDS             25018..26481
                     /gene="bepA"
                     /locus_tag="ACIUS0_001352"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGB0219168.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26502..26861
                     /gene="arsC"
                     /locus_tag="ACIUS0_001353"
     CDS             26502..26861
                     /gene="arsC"
                     /locus_tag="ACIUS0_001353"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGB0219169.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26994..27695)
                     /locus_tag="ACIUS0_001354"
     CDS             complement(26994..27695)
                     /locus_tag="ACIUS0_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGB0219170.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27790..29079)
                     /gene="uraA"
                     /locus_tag="ACIUS0_001355"
     CDS             complement(27790..29079)
                     /gene="uraA"
                     /locus_tag="ACIUS0_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGB0219171.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29165..29791)
                     /gene="upp"
                     /locus_tag="ACIUS0_001356"
     CDS             complement(29165..29791)
                     /gene="upp"
                     /locus_tag="ACIUS0_001356"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGB0219172.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30115..31152
                     /gene="purM"
                     /locus_tag="ACIUS0_001357"
     CDS             30115..31152
                     /gene="purM"
                     /locus_tag="ACIUS0_001357"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGB0219173.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31152..31790
                     /gene="purN"
                     /locus_tag="ACIUS0_001358"
     CDS             31152..31790
                     /gene="purN"
                     /locus_tag="ACIUS0_001358"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGB0219174.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            31962..34028
                     /gene="ppk1"
                     /locus_tag="ACIUS0_001359"
     CDS             31962..34028
                     /gene="ppk1"
                     /locus_tag="ACIUS0_001359"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGB0219175.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34033..35574
                     /gene="ppx"
                     /locus_tag="ACIUS0_001360"
     CDS             34033..35574
                     /gene="ppx"
                     /locus_tag="ACIUS0_001360"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGB0219176.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVAGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35613..>36722)
                     /gene="pdeF"
                     /locus_tag="ACIUS0_001361"
     CDS             complement(35613..>36722)
                     /gene="pdeF"
                     /locus_tag="ACIUS0_001361"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGB0219177.1"
                     /translation="YKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALD
                     SHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQR
                     CGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDEL
                     IGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFP
                     VEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYAS
                     YARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIRE
                     AVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8507 a         9714 c         9547 g         8954 t
ORIGIN      
        1 caggcaaaat gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca
       61 ttatggttac actttacggc atcaaaaatt gtgacaccat taaaaaggct cgccgttggc
      121 tggaagccaa taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg
      181 aattattgaa cggttttatc aacgaattag gctgggaagc gttactcaac acccgtggta
      241 caacctggcg taaactggac gaaactaccc gcaataaaat caccgatgcg gcctctgcgg
      301 cggcattaat gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gcgcctggta
      361 agcctatgct gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt
      421 ctatgtcgtg cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc
      481 ctgatgacgc aggatgtcag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg
      541 ttgaacgcat ggattttgcc gatacgcaga atttttgggc atggcgtggg caaggtgaaa
      601 cgttagcctt tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca
      661 atccgccgtt tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata
      721 tgaaaggctc gctggcggcg atggtggtag cagcagaacg ttttgtcgca caacatccca
      781 accatacggg gcgactggca tttctgatca cttctgatga agaagccagt gcccacaacg
      841 gtacggtaaa agtcgtcgag gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg
      901 tcggcgaacc gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat
      961 cgttaacctg caacctcacc attcatggcg ttcaggggca tgttgcctac ccacatctgg
     1021 ctgacaatcc ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg
     1081 atcagggcaa tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa
     1141 cgggcagtaa caatgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca
     1201 ccgaactgac tgatgagatg atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac
     1261 tgcgctatac ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac
     1321 tggtggatgc ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga
     1381 ccacaggcgg aacgtccgac gggcgcttta ttgctcgcat gggggcgcag gtggtggaac
     1441 tcgggccggt caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc
     1501 agctacttgc ccgtatgtat caacgtatca tggaacagct cgtcgcgtga tgagtggttc
     1561 tgcaagagga aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct
     1621 ttttggtagg cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac cataagaaat
     1681 ttctcgccaa caagccggag cttcccccgc atcgtgattt caacgataag tgggacgatg
     1741 acgacgactg gccgaaaaag gatcaaccga agaagtaagc tgagtgctca accgtaggcc
     1801 ggataaggcg ttaacgccgc atccggcaat ggtgaacgat ccctgatgcg acgctcacgc
     1861 gtcttatcag gccaggtaat ctgtctttta catcatttca atcacatcat catcgccagg
     1921 cttaccgcca cttagcgctt ggtcgaaata atgtttcgga atggtataac gcaagtgatc
     1981 cagggcaaat tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag
     2041 cgtcacgtca ccaccggcac tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc
     2101 gataaccgga tcttcaccac catgaatcaa gtgaattgtc gtggctgtag acgcggtctc
     2161 cggcaggctc gcatagcgtc cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc
     2221 tttaatgctc tccagcgcca taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt
     2281 agcccctact ccgctctgtt tctgccagta gcgtaccgtt tcaataaacg tcggcatgat
     2341 ggcatccaca cgcgcctggc gattatcttc cgtaataccc tgcactgaaa accactgacg
     2401 ccccgccgga ttaccgctcg gctccgcgcc accgacactc accaccagcg catcaggaaa
     2461 cagcggtgca aaccagctgc ctatttcccc cattgccacc gggttatccc cgacaccgtg
     2521 gaaaagcagt aacaactgtt gtgcaggttt atccgggctt tgaacaacaa aatggtcatg
     2581 tttcatggcg aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca
     2641 tgggagaaaa ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcagac
     2701 gctcagtgcg aacctcattt agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa
     2761 ccagtaacat cttgcggcct gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt
     2821 ttttctgtaa acgcccacgc agcgccggta atgccagttc actggtttgt agcagacgca
     2881 ataagcaacc taacgatgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa
     2941 gccagtcgtc gtcagaaagg atggcatcgt ttagtggatc aacaggaagc atttcgccat
     3001 tccactgcgc gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct
     3061 gtttacccgc atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt
     3121 cccgatgatt acccatccgc accagcacaa aaccgcagcg ttgccagaaa cgccataact
     3181 ccccagtgta accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagctc
     3241 cagcaataag ttgtcgccct gtgccttccc gctgacgagc cggatgaact gctatccggc
     3301 taacccgcct tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact
     3361 gggccaccag attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt
     3421 gagataatcc gccctcatcc accagccaca acgccccggc aatctcgttt tcgccagccg
     3481 cctgtaaaaa atgttgccct ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc
     3541 ggtagtgcgc accagacaat aactgataaa cctttaacgg cgtttctggc tcgcttcgcc
     3601 ataacgtttg ttcaaatgcg gaaatgacga tattgccttg tggtgtatgg gtgaagtttt
     3661 catcgttaaa aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc
     3721 ggatcggctg ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca
     3781 aaaaaccacg tccggtacct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa
     3841 aacgcgatac cagttgatgc aacaacggcg caggtatggc tgcggcttcg tcgaccacca
     3901 gccagtcggc ttgctcatcg ctggctaaca aggcatccgg tgcaataaag cgaaacttct
     3961 cgcccgcaaa ttgtgccagt acatccgttg ccgcttttgc gggcgcggtg acaatcgcac
     4021 tgcccgcaat acgagaaatg agttgccctg ccaacgccga cttaccgcgc ccacgcgcag
     4081 ccgttaccgc tgccacgccc ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt
     4141 ctggttgtgg tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg
     4201 agaacggctg gttttgccgc cagaggatag cgtcgttatc cgccgtaagt acgcgtttga
     4261 gatgctggac aaaatgcggc gtcgcaatag ggtcagggca atcactccag cgcagcgagt
     4321 cggcatcagg ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc
     4381 ccgctttcaa cgttccgcta agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt
     4441 cgaataccgc atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt
     4501 ggttttcagc atctagctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca
     4561 acttaagagc atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcaga
     4621 tcccttcacg tttcatttgc gctgttaatg tgtaaagcgc agtcagttca gccatccctg
     4681 ccccggaaat taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa
     4741 accacgttta aaccagctgt agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac
     4801 tactcgcccc tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag
     4861 cgcctcttcc agatcgccag tttccagaac gccttgctgc tgcatactat gcccccagac
     4921 accggcaaaa cagtcggcct ggagttccat acgcacagat aagcggttta cttccgcctg
     4981 cgtcgcgttt tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg
     5041 atgaccgact tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt
     5101 gtctttcatg tcatcataga aggagagatc gatataaact gtgccatccg ccgggcaata
     5161 gaacggcccc ataatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat
     5221 gaccagcttc ggttgctgat aggtcttacc catcttttcg aactgttgtc cccaggtgtc
     5281 ttccgtggtt gcctgaatca ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat
     5341 tgaccgcgtt gattgttgtt gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac
     5401 gccatagtag cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgctcgg
     5461 cagacggaaa ccgggaccgc ccattgacgg accgccagag ctgttgcgcc tgtcttcaac
     5521 attgtcactt tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata
     5581 gaaatgatcg taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga
     5641 ggaatgagcg ttcaatgcat atattccaag agcggttgtc ttgccgtgcc agctgcacgg
     5701 caagatgatg aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca
     5761 taggcttcga tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagt
     5821 gtttctttgt cccacaggcg gctgccgtcc ggggagaact catcgcccag taccacttca
     5881 cctttgtaca gaccaaactc cagcttgaag tcgaccagaa tcagaccagc atcatcgaac
     5941 agttttttca gcacgtcgtt cgctttgtag gtcagctctt tcatacgcgc caggttctct
     6001 ttgctcaccc agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggca
     6061 tcgtttttca ggaacagatc gaacagcggc gggttcagct caataccttc ttcgattcca
     6121 agacgtttca ctagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata
     6181 tccagctttt tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata
     6241 cccgcttcag ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg
     6301 cgatcaaact gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc
     6361 accaacaggt ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca
     6421 gcttgctttt gcatctttat cactcctggg tgtgaattaa cgttttaaaa tcttttgctg
     6481 tctggtgtgc cggatgtttt gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga
     6541 caaacaaatt tcgtgcgaat taccgctaaa atcgcgtttt cctgccgacg cacacgtttg
     6601 cgtatcatat cagaaaaaag ggccggatga ttccagccct gtatttttac ttgctaaacg
     6661 cagcctggaa gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg
     6721 gatcgatgaa ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac
     6781 cggatgccag gcctggatcg ctcgcgccca gttcctgcca gtcgctgtca gacagcggct
     6841 tataagttac ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt
     6901 tttccagcgc cgctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca
     6961 ttggtaaacc ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt
     7021 gcgcagcgtt cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca
     7081 tctccgtgct gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca
     7141 ggttcagcag tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat
     7201 aacgaccacg atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca
     7261 gtgtctgacc agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa
     7321 ccacctgcgg ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc
     7381 ccgtgaactg ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt
     7441 ccagcgcctt accgacagca ccactaccgt tggtcaccgg gattgcataa tcaccggagg
     7501 tcaccggcaa aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt
     7561 aggcttcatc accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta
     7621 ataaaacaag cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca
     7681 tcaaatctcc ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca
     7741 cgaccactgt cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc
     7801 agttccttac atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt
     7861 aatggcatca gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt
     7921 ttgcacatct gggccatatc acgcgctgcg acgttagccg taacggaaat aactccatga
     7981 ccgcccaatt gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca
     8041 tctgaaacca gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt
     8101 ccgataatat tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca
     8161 gtacgggacg gcacattata cagaatttgc ggcaggtcag tatgctcagc gatggctttg
     8221 aaatgctgat acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag
     8281 ccgacgatac cactgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta
     8341 gcgccggtcc cggcaattac cggaatgcgc ccatcagcca gatccagcgt catcatcacc
     8401 acatcagcat gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg
     8461 atcgccgaag taccgctggc gacatgataa tcaatcagtt ttttcaagct agcccgacag
     8521 acattacctt tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc
     8581 catcctctgt gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag
     8641 acagaaccgt tctgattgtt gtatgcatga tttttttatg ctttccttaa gaacaactca
     8701 ccccttaaag gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg
     8761 ttgggtgccg atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc
     8821 tgtaatattg aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg
     8881 ctttccggtt catggaatgc catcactctg attgaatcaa cgttaccgtt gaaaggtgcc
     8941 gaactggatc ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca
     9001 gcatctgtct gggttcaggt cgatgttgca gactccccgc atttaattga acgcttcaca
     9061 gcacttttcg acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac acaacctgct
     9121 gaaaatgaac gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg
     9181 gacgcagcaa atattgagca agcgttcaaa gccctatgta cagaactcaa tgcacaaggc
     9241 agtattaacg tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc
     9301 cactgaaagc cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag
     9361 ttaatttgac cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga
     9421 cccccggctg taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag
     9481 cgggcgttga tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg
     9541 aaaaagagct gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat
     9601 tcggcgtctg gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca
     9661 gcttcctgat tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca
     9721 atcaccacga cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc
     9781 cgtgctggct gcgcttgcgg ccagcatacc tcacttctcg tgatcaagat cacattctcg
     9841 ctttcccctg cgacacgggt gtcgaatcca ttttttgctg aacgttaatg accatcattt
     9901 ttgtaccgtt cagaatccag ttaatacata acttattgaa tagattgagt taatcagaat
     9961 ggcatccttt atacaatatg aaatgcaatg tttcatatca ttttcaagga gccgacatga
    10021 accgctttgt ggtggccgaa ccactgtggt gtacaggatg taatacctgt ctcgctgcct
    10081 gttcggacgt gcataaaacg caaggtttac agcaacaccc gcgcctggcc ctggcgaaga
    10141 cgtcaacaat cactgcccct gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg
    10201 tctgcccggt caatgccatc tctcagaggg atgatgcgat ccaactcaac gaaagcctct
    10261 gtattggctg caagctttgc gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa
    10321 gccgtccggt gaatgcccat gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg
    10381 gcgaagaaaa cgtgccgccc caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg
    10441 tcgcggtgaa atgcgacctg tgtgatttct tgccagaagg tccggcctgc gttcgcgctt
    10501 gcccgaatca ggcgttacgg ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa
    10561 aacagcgcct tgccgcaagc tggtttgcca atggcgggga gtctcccctt tccctcactc
    10621 aggagcaaca ctaatggatg ccctgcaatt attaacctgg tcgctgatcc tctatctgtt
    10681 tgccagtctg gcttcgctgt ttttactcgg tctggacaga ctggctatta agctttccgg
    10741 catcacatcg ctggtgggcg gcgtgattgg catcatcagc ggaattacgc aattacatgc
    10801 tggtgtaact ttagtcgccc attttgccac cccttttgac tttgccgatt taaccctgcg
    10861 aatggatagc ctctcggcat ttatggtgct agttatctcc ttgctggtgg tggtttgttc
    10921 gctctattca ttgacttata tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt
    10981 ctttatgaat cttttcatcg catcgatggt tgccctgctg gtgatggaca acgctttttg
    11041 gttcatcgtg ctgtttgaaa tgatgtcgct gtcttcctgg tttctggtca ttgccaggca
    11101 ggataaaacg tcgatcaacg ctggcatgct ctactttttt atcgcccacg ccggatcggt
    11161 gctgattatg atcgccttct tgctgatggg gcgcgaaagc ggcagcctcg attttgccag
    11221 tttcgcacgc tttcactttc tccggggctg gcgtcggcgg tgtttctgct ggcctttttc
    11281 ggttttggcg cgaaagccgg gatgatgccg ttgcacagct ggttgccgcg cgctcaccct
    11341 gccgcaccat cgcacgcttc ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc
    11401 ggcatcctga aagtagcgat ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc
    11461 attctggtga tggcgatcgg cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg
    11521 gaacaggata tcaaacggct gctggcctgg agtaccgtcg aaaacgtcgg cattattttg
    11581 ctggcagtcg gtgtggcgat ggacggtctg tcactgcacg acccgctgct caccgtggtt
    11641 ggactgctcg gcgcactgtt tcatctgctc aaccatgcgc tgttcaaagg gctgctattt
    11701 ctcggcgcgg acgcgattat ttcgcgtttg catacccacg acatggaaaa aatgggggca
    11761 ctggcgaaac ggatgccgtg gacagccgca gcatgcctga ttggttgcct cgcgatatca
    11821 gccattcctc cgctgaatgg ttttatcagc gaatggtaca cctggcagtc gctgttctca
    11881 ctaagtcgtg tggaagccgt agcgctacaa cttgcgggtc ctattgctat ggtaatgctg
    11941 gcagtcactg gtgggctggc agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt
    12001 ggtgcgccgc gcagtacaca cgctgaagag gcacaggaag tgccaaatac gatgatcgtc
    12061 gccatgctac tgctcgcggc actctgcgta ttaattgcgc ttagtgccag ttggctggca
    12121 ccgaagataa tgcatattgc ccatgcgttt accaataccc ctcccgtcac tgtcgccagc
    12181 ggaatagcac ttgtacccgg cacgtttcat acacgggtca ctccttcatt actgttgctg
    12241 ttactactgg cgatgccttt gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc
    12301 gcagcgtttc gtcgcacagg agatgcctgg gcatgcggct acggctggga aaacgcgatg
    12361 gctccgtcag gcaatggcgt gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt
    12421 ctacgacaac agctcgaccc tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc
    12481 agggctcaga gcacagaacc cttctgggat gagcgggtga tccgccccat cgtgagcgcc
    12541 acccaacggc tggtcaaaga aatacagcat ctgcaaagcg gcgactttcg tctctattgc
    12601 ctgtatgtgg tcgccgcact ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac
    12661 catgagacaa actctttgcg acggatatct ggtcattttt gcgttagcac aggccgtgat
    12721 tctgctgatg ctaaccccac tttttacggg tatttcccgg cagatacgcg cgcgtatgca
    12781 ctcccgccgc gggccgggga tctggcagga ttatcgcgat atccacaaac tgtttaaacg
    12841 ccaggaagtt gcgccgacat cttcaggtct gatgttccgc ctgatgccgt gggtattaat
    12901 cagcagcatg ctggtgctgg cgatggcctt accactattt attaccgttt ccccttttgc
    12961 gggcggcggc gatctgatca cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc
    13021 tctttccggg ctggataccg gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac
    13081 gctcggcatt ctggtcgaac caatgcttat tctctcactg ctggtattgg cgctgatagc
    13141 aggttccacg catatcgaga tgatcagcaa tacgctggct atgggctgga actcgccgct
    13201 aaccaccgta ctggcgttac tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat
    13261 tccctttgat gttgctgaag cagaacagga attacaggaa ggcccgctga ccgaatattc
    13321 cggtgccggg ctggcgctag cgaaatgggg gctggggctg aaacaggtcg tgatggcagc
    13381 actgtttgtg gccctgtttc tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct
    13441 gctgacttca cttgtcgtta cgctgctcaa ggttttgctg atttttgtac tggcctcaat
    13501 cgcagaaaac acgctggcac gcgggcgttt tttactcatt caccatgtga cctggcttgg
    13561 cttcagcctt gctgcgcttg catgggtctt ttggttaacc ggtctgtaag gagcactgac
    13621 ggaatatgga aaatcttgct ctgacgacgt taatgctgcc ttttatcggc gcactggtcg
    13681 tttcgttttc gccacaacgt cgggccgccg aatggggggt tttgttcgcc gcgctgacca
    13741 cgctgtgcat gttgtcgctg atctccgcgt tttatcaggc cgataaagtt gccgtcacgt
    13801 tgacgttggt caacgtgggg gatgtggcat tatttggcct ggtcattgat cgcgtgagta
    13861 cgctgattct gtttgtggtg gtgtttctcg gtttgctggt cacgatctac tccacgggtt
    13921 atctgacgga taaaaatcgc gaacacccgc ataacggcac gaatcgttat tacgcatttt
    13981 tgctggtgtt tatcggcgcg atggcgggac tggtactctc ctcgacgctg ctcggtcagt
    14041 tgttgttttt tgaaattacg ggcggctgct cctgggcgtt gatcagttat taccagagcg
    14101 ataaagcgca gcgttcagca ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt
    14161 tgtatcttgc cgccgccacg ctgtttttgc agaccggaac gtttgtgctt agcgcgatga
    14221 gcgagttaca tggcgacgca cgttatctgg tttatggcgg catcctgttt gccgcgtggg
    14281 ggaaatcggc ccagctaccg atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac
    14341 cgatcagcgc ctatctccac gccgcatcga tggtgaaagt gggcgtttac atttttgccc
    14401 gcgccattat cgacggcggc aatatcccgc atgtgattgg cggcgttggc atggtcatga
    14461 cactggtcac cattctttat ggctttctga tgtatttgcc acagcaggat atgaagcggt
    14521 tgctggcctg gtcgaccatc actcaacttg gctggatgtt cttcggcttg tcgctctcca
    14581 tcttcggctc gcggctggcg ctggagggta gcatcgccta catcgtcaac cacgcgttcg
    14641 ctaaaagcct gtttttcctt gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt
    14701 tgccgcgtct gcgtggcgta ttgcacaccc tgccgttgcc aggcgtgggt ttctgcgtgg
    14761 cagcgctggc aattaccggt gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt
    14821 ttgccgccgg ttttgcgttg tcagtggagt actggatcct gctgcccgcc atgattctgc
    14881 tgatgattga atcggtcgcc agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc
    14941 cgggcaaacc gagcgaggcc gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg
    15001 tgttgattgt actgattgtg atgtcgctga tttccagcgt aatcgccgcg acctggttgc
    15061 agtaaggaga tgatgaatga ccggttctat gatcgtaaat aatctggcgg gactgatgat
    15121 gctgacatcg ctgtttgtga ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc
    15181 ctgccagtcg ctggtgctgg tgtctatttt cgccactctc tcgtgcctgt tcgccacaga
    15241 gcaactgctg atctggtccg ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat
    15301 catgacttac gctgcacgaa atattcccca gaacatcccg gaaaaagcgt tattcggtcc
    15361 ggcaatgatg gcactgctcg cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc
    15421 cgtgaagcta ccgatggcta ccgggctgaa accggcgctg gcggtagcgt taggtcattt
    15481 tctgcttggc ctgctgtgca ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta
    15541 ctgcctgatg gaaaacggct cccatctggt gctggcgctt cttgcctggc gagcaccgga
    15601 actggtggaa ataggtatcg ctaccgacgc catcttcgcc gtcattgtga tggtgttact
    15661 ggcaagaaaa atatggcgta cccacggcac gctggacgtg aacaacttga ccgcgctgaa
    15721 gggataatga gatgagttat tctgtgatgt tcgctttact cctgctcacg ccgctgcttt
    15781 tttcgctgct ctgttttgcc tgccggaaac gggggctctc tgcgacttgt acggtgactg
    15841 tattacatag cttagggatc acactgctgc tgattctagc actctgggtg gtccaaactg
    15901 ccgctgatgc aggagaaata ttcgctgcgg gactgtggct tcatattgat ggtctgggcg
    15961 gtttgttcct cgccattctt ggtgtgattg gctttctcac cggtgtttac tcgattggct
    16021 acatgcgtca tgaagtggca cacggcgagc tttcacccgt tacactgtgc gattactacg
    16081 gtttcttcca tctgtttttg ttcaccatgc tgctggttgt taccagcaat aacctgattg
    16141 tgatgtgggc ggcgatcgaa gccaccacct taagctcggc gtttctggta ggcatttacg
    16201 gtcagcgttc atcgctggaa tcagcatgga agtacatcat tatttgtact gttggtgtcg
    16261 cttttggtct gttcggtacc gtgctggtat acgccaacgc cgccagcgtt atgccgcagg
    16321 cagaaatggc gatattctgg agcgaggttc ttaagcaatc gtccttgctt gacccaacat
    16381 taatgctgtt ggcctttgtg tttgtgctaa ttggctttgg taccaaaacc gggctatttc
    16441 ccatgcacgc ctggctgccg gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc
    16501 tctccgccgt attgctgaac tgcgcgctgt tggtgctgat tcgctattac atcattattt
    16561 gccaggccat cggcagcgat ttccccaacc ggttgttgct catcttcggc atgttgtcgg
    16621 ttgccgtggc ggcatttttc attctggtac agcgggacat taagcgtctg ctggcgtact
    16681 ccagcgtgga gaacatgggg ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa
    16741 tttttgccgc gctgctgcac accttaaacc acagtctggc aaaaacgctg ctgttctgcg
    16801 gttccggcaa tgtactgctc aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc
    16861 tcaaaatcat gccatttacc gccgtgctgt ttggcggcgg tgcgctggcg ctggcgctgg
    16921 cagggatgcc gcccttcaac atttttctta gcgaatttat gaccgttacc gcaggactgg
    16981 cacgtaatca cctgctgatt atcgtcctgc tgttattgct gttaacgctg gtgctggcgg
    17041 gcctggtacg gatggctgcg cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg
    17101 gtgatctcgg ctggttgacc acctcgccaa tggtgattct gctggtcatg atgctggcga
    17161 tgggaacgca tattccacaa cctgtcatca ggatcctggc gggcgcttcc actatagtcc
    17221 tctcagggac gcacgacctg cctgcacaac gtagcacctg gcatgatttt ttgccttcag
    17281 gcaccgcatc tgtttcggag aaacacagtg aacgttaatt catcgtcaaa tcgtggcgaa
    17341 gcgattctcg ccgccctgaa aacgcagttc cccggcgcgg tgctggatga agagcgacaa
    17401 acgcctgaac aggtcaccat tacggtgaaa atcaatctgc tgcctgacgt tgtacattat
    17461 ctttattatc aacatgatgg ctggcttcca gtcctgtttg gcaacgacga gcggacactt
    17521 aacggtcatt acgcggttta ttatgccctt tcaatggaag gggccgaaaa atgctggatc
    17581 gtggtgaagg cactggtcga tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc
    17641 cctgccgcgg tctggggcga gcgagaaatt cgcgatatgt acgggctgat tccggttggc
    17701 ctgccggatc agcgtcgcct ggtgttgccc gatgactggc cggaagatat gcatccgctg
    17761 cgcaaagatg cgatggatta tcgactgcgc cctgaaccga cgacagattc cgaaacgtat
    17821 gcgtttatca atgagggcaa cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc
    17881 acctcctatg aaccgggtca cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct
    17941 gattaccgcc tgttttatgt ccatcgcggc atggagaaac tggcagaaac gcggatgggc
    18001 tacaacgaag tgaccttctt atcggaccgc gtgtgtggga tttgcggttt tgcccacagt
    18061 gtggcctata ccaactcggt tgaaaatgca ctggggattg aggtgccgca acgagcacat
    18121 actattcgct cgattctgct ggaagtcgag cggctacaca gtcatttgct caaccttggc
    18181 ctctcctgcc attttgttgg ttttgatacc ggctttatgc aatttttccg cgtgcgggaa
    18241 aagtcgatga cgatggcgga attgctgacc gggtcgcgta aaacctacgg tctgaatctg
    18301 attggtggtg ttcgccgcga tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg
    18361 cgcgagatgc gcgccgacgt gtcggagctg gtagagatgc tgcttgctac gccgaatatg
    18421 gaacaacgca ctcagggcat tggcattctc gaccgacaaa tcgcccgtga ttatagccct
    18481 gtagggccgc tgatccgcgc agtggttttg cccgtgattt gcgctttgat cacccctacg
    18541 ccgactacgg caatattcca aaaacactgt ttacctttac cggcggcgat gttttctccc
    18601 gcgtgatggt ccgtgtcaaa gagacgtttg attcgctggc aatgctggaa tttgccctcg
    18661 acaacatgcc ggatacccca ctgctgaccg aaggctttag ctataaacct cacgcattcg
    18721 cgctgggctt tgttgaagcg ccacgcggtg aagacgtgca ctggagcatg ctcggtgata
    18781 accaaaaatt gttccgctgg cgctgtcgtg ccgccaccta cgccaactgg ccggtgttgc
    18841 gttacatgct gcgcggcaat accgtttctg acgcaccgct gattatcggt agccttgatc
    18901 cctgctactc ctgtaccgac cgtgtgacgc tggtagatgt gcgcaagcgc cagtcaaaaa
    18961 ccgtgctgta taaagagatc gaacgctacg gcattgatcg taaccgttcg ccgctgaagt
    19021 aaggacagaa gatgctgaag ttactgaaaa ctattatgcg cgccggaacc gcgacggtga
    19081 aatatccctt cgcgccactg gaggtcagcc caggctttcg cgggaaaccg gacctgatgc
    19141 ccagccaatg tattgcctgc ggtgcctgcg cctgtgcttg tccggcaaat gcgctgacta
    19201 tccagaccga cgaccagcaa aattcgcgca cctggcagct ctatctgggg cgttgtattt
    19261 actgcggacg ttgtgaagaa gtgtgcccga ccagagccat ccagcttacc aataactttg
    19321 aactgaccgt cacccataaa gccgatctct atacccacgc gacgttccat ctacaacgtt
    19381 gcagccgttg cgaacgcccg tttgccccgc aaaaaaccgt cgcactggct gctgaattgt
    19441 tggcacagca acaaaatgct ccacaaaacc gcgaaatgtt atgggcgcaa gcgagcgtct
    19501 gcccggagtg caaacaacgc gcaacgctga tcaacgacga tacagatgta ccgctggtgg
    19561 ctaaggagca gctatgagtc cagtgcttac gcaatatgtc agccagccca tcacgctgga
    19621 cgagcaaacg caaaagatga agcggcattt gctacaggat atccgtcgct cggcttacgt
    19681 ttatcgcatc gattgcggcg gctgcaacgc ctgtgaaatc gaaatttttg ctgccatcac
    19741 ccccgtattc gacgcagaac gttttggcat taaggttgtt tcatcaccgc gtcacgccga
    19801 tattttgtta tttactggcg cagtcacccg ggcgatgcgt atgcctgcac ttcgggcgta
    19861 tgagtctgcc cccgatcata aaatctgtgt ttcctacggc gcgtgcggtg tcggcggcgg
    19921 tattttccac gatctctaca gcgtctgggg cggcagcgac accattgtcc ccattgatgt
    19981 ttggatcccc ggctgcccgc caacaccggc cgccaccatt cacggtttcg ccgtggcgct
    20041 cggtttgctg caacagaaga ttcacgcggt ggattatcgc gatcccaccg gggtgactat
    20101 gcaaccgttg tggccgcaga tcccgccatc acagcgtatc gccattgagc gagaagcgcg
    20161 gcggctggcg ggctatcgtc aggggcgaga aatttgcgat cggctcctgc gccatttaag
    20221 cgacgatcct acaggaaatc gggttaacac ctggttgcgc gatgccgacg atccacgtct
    20281 caatagtatc gttcagcaac tctttcgcgt actccggggg ttacatgact gaagagtgcg
    20341 gggaaattgt tttctggacg ctgcgaaaaa agtttgtcgc cagtagcgac gagatgccgg
    20401 aacacagctc tcaggtaatg tattactcgc tggctatcgg tcatcacgtt ggcgtgattg
    20461 attgtctgaa tgtcgccttc cgctgcccac tgacggaata cgaagattgg cttgcactgg
    20521 tcgaagagga gcaagcccga cgtaagatgc tgggggtgat gacttttggt gagattgtta
    20581 ttgacgccag ccacaccgcc ctgttgaccc gggcattcgc gccactggca gatgacgcga
    20641 cgtctgtgtg gcaagcgcgt agcattcaat tcattcatct gttggatgaa attgtgctgg
    20701 aaccggctat ctatctgatg gccagaaaaa ttgcgtgaga aggatttctc attaataagg
    20761 actgttgatg gctatgtcag acgaagcgat gtttgccccg ccgcaaggaa taacaattga
    20821 agcggtaaac ggaatgctcg cggagcggtt agcgcagaaa cacggcaagg cgtctttatt
    20881 acgcgccttc atcccgctgc cgccgccgtt cagcccggta caacttattg aactgcatgt
    20941 tctcaaaagc aacttctatt accgctacca tgatgatggc agcgatgtga cggcaacaac
    21001 agagtatcag ggcgagatgg tcgattattc gcgttacgcc gtccttctcg gcagtagtgg
    21061 aatggcggag ctacgcttta ttcgtaccca cggcagccgt tttactcccc aggattgcac
    21121 actgtttaac tggctggcac gcattatcac cccggttctg caatcatggc tcaatgatga
    21181 agcacagcag gtggcgctgc gtttgctgga gaaagatcgc gatcatcatc gggtactggt
    21241 ggatatcact aatgcagtgc tgtcacatct tgatctcgac gatctgatcg ctgacgtcgc
    21301 tcgtgagatc catcattttt tcggtctggc ttcagtcagt atggtactgg gcgatcatcg
    21361 aaagaacgag aagttcagcc tgtggtgcag cgatctttct gcctcacatt gtgcgtgtct
    21421 gccacacaat atgcctggcg acagtgtatt gctgacacaa acgctacaaa cccgacaacc
    21481 gaccttgacg caccgtgcag atgatctgtt tctctggcaa cgcgacccgt tattactctt
    21541 acttgcatct aacggctgcg aatctgcgct ccttataccg cttacctttg gcaaccatac
    21601 accgggtgca ttgttgctgg cgcatacctc ttccactctc tttagtgagg aaaactgcca
    21661 gctactacaa cacatagccg atcgcatcgc tattgccgtt ggcaatgccg atgcctgccg
    21721 tagaatgacc gatttgcagg aaagtttgca gcaagaaaac caccagctta gcgagcagct
    21781 cctttcgaat ctgggcgtcg gtgacattat ctatcaaagc caggcaatgg aagacctgct
    21841 ccagcaggta gatattgtgg cgaagagcga cagtacggtg ttgatttgcg gtgaaaccgg
    21901 aaccggcaaa gaggtgatcg ccagagcgat ccatcaactt agcccgcgac acgacaagcc
    21961 gctggtcaaa atcaactgcg ctgccatccc cgccagtctt ctggaaagtg agttattcgg
    22021 tcatgacaaa ggggcgttta ctggtgcgat taatacccat cgtggtcgtt ttgaaattgc
    22081 cgatggcggc acgttgtttc tcgatgaaat tggcgatctg ccgttagaac ttcagcctaa
    22141 actgctgcgc gtattgcagg aacgggagat tgagcgtctc ggcgggagta gaacgatccc
    22201 ggtaaatgtc agagtcattg ccgccaccaa ccgtgatttg tggcaaatgg ttgaagatcg
    22261 ccagtttcgc agcgatctct tttatcgcct gaatgtcttc ccactggaat tgcctccgct
    22321 gcgcgaccgt ccggaagata tccctctttt agcaaagcat ttcacgcaaa aaatggcgcg
    22381 ccatatgaat cgttcaattg acgccatccc gaccgaggcg ctacgccagt tgatgtcgtg
    22441 ggactggccg ggcaacgtgc gcgagctgga aaacgtgatt gagcgagcgg tactgctgac
    22501 tcgcggtaac agtctgaatt tacatcttaa tgtccgacaa agccgtttac tgccgacgct
    22561 aaatgaagat tcagcgcttc gcagttcaat ggcgtagttg ctgcacccga cgacgccaga
    22621 gaatgacgaa gaagaacgtc agcgcattgt tcaggtattg cgagaaacca atggcattgt
    22681 tgccgggccc cgtggcgcgg cgacacgatt agggatgaag cgcaccacgc tgctgtcacg
    22741 aatgcagcgt ctgggaatct cggttcgcga ggtgttgtaa tctgcttttg caggagtatg
    22801 catgagaaac aaactctctt tcgacttgca gttgagcgcc agaaaagcgg caatcgctga
    22861 acggattgcc gcccataaaa ttgcccgcag taaagtgtcg gtctttttaa tggcgatgtc
    22921 cgctggcgtg tttatggcga tcggatttac tttttacctt tccgttatcg ccgatgcccc
    22981 gtcttcatag gcattaaccc atctggtggg cggcctttgc tttacacttg gctttatttt
    23041 gctggcggtt tgcggcacca gcctgttcac ctcgtcggta atgacggtaa tggcaaaaag
    23101 tcggggcgtt attagttggc gaacttggct gattaacgca cttctggtgg cctgcggtaa
    23161 tctggcaggt attgcctgtt tcagtttgtt aatctggttt tccgggctgg tgatgagtga
    23221 aaacgcgatg tggggagtcg cgattttaca ctgcgccgag ggcaaaatgc atcatacatt
    23281 tactgaatct gtcagcctcg gcattatgtg caatctgatg gtttgcctgg cgctgtggat
    23341 gagttattgc ggacgttcgt tatgcgacaa aatcgtcgcc atgattttgt ccatcaccct
    23401 gtttgtcgcc agtggctttg agcactgtat cgccaatttg tttgtgattc cgttcgccat
    23461 tgccattcgc catttcgccc ctacctcctt ctggcaactg gcgcacagta gcgcagacaa
    23521 ttttccggca ctgacggtca gccattttat taccgccaat ctgctcccgg tgatgctggg
    23581 taatattatc ggcggtgcag tgctggtgag tatttgttat cgggctattt atttacgtca
    23641 ggaatcctga aaaatcagcc cggcgaaaca gttcgtcggg ctatttattt acgtcaggaa
    23701 tcctgaaaaa tcagcccggc gaaacagttc gtcgggctga agattattct tgcgcgattt
    23761 gcccatcggg ccaggcgtgg atcacggctt tgatcagcgt cgccaatgga atggcgaaga
    23821 atacgcccca gaatccccac aaaccaccga agatcaccac cgataaaata atcaccagcg
    23881 gatgcaggtt aaccgcttcg gagaacaaca ccggtactag caggttgccg tccagcgcct
    23941 gaataatcag atacaccgcg aagcagctcc agaattcagt acctgcgcca aactggaata
    24001 gcgccacgcc aaccaccgga atggtcacca caaatgcgcc aatgtacgga ataagaaccg
    24061 agaagccgac cagcactgcc agcagcagcg aatagttcag cccgaagagc aagaacccca
    24121 gccaggtggc gatccccacc acgatcatct ccagcacttt gccgcggata tagttggtga
    24181 tttgttgatt catctccttc cacacctgtc ctgccagccc acggttacgc ggcagcaccc
    24241 ggcgaacggc gttcagcatc tgctctttgt ctttcagcag gaagaagacc atcaatggca
    24301 ccagcaccag atagacggct atggtcagca aaccgaccag cgaggcgagg gaaattttca
    24361 ccaccgaatc gcccatggtc agcatccgac tgcgcatatt ttcggccatt gcatcaataa
    24421 tgcccgcgtc cattaacacc ggatagcggc gcggcaacgt ggcggcaaag tcagaaagct
    24481 tattgagcat ccccggcata tcgcggatta agtagatgcc ctgttgccag gcgataggca
    24541 gtaccacgaa cgccatcagt agcaatatac cgacaaaaac caccaataca atcgacgtcg
    24601 cccagcggcg ggagcagcca atagattgca ggcgcacggt tggccattcc agcaaataag
    24661 ccagcacaat agccaccagc aacggagcaa gcaggccact aaagaaaaag ataatgccaa
    24721 atccggcaac taaaataacc agcaaggcaa tcgcttccgg gtcgctaaaa cggcggcgat
    24781 accattgcat caacatttcg agcataaaga ccttccctga acctcaagag cgggattgcg
    24841 atccgcaatt gtatcgaaat gtcacaaaaa agacttcgct ttttatgacg gattcaggaa
    24901 actgaaaagt catttgagtg ggctaatctt cgccgttaca ctcaaaggcg gcgaggtggg
    24961 aacgatattt cacagtatcg gtcaaatgac tatcttcaga aatacaggat agaggttatg
    25021 ttcaggcagt tgaaaaaaaa cctggttgca accctcattg ctgctatgac cattggtcag
    25081 gtagccccgg cgtttgccga cagcgcagac accttgccgg atatgggaac ctccgcagga
    25141 agcacgcttt ccattggtca ggaaatgcag atgggcgact attatgtccg ccagctacgc
    25201 ggcagcgcgc cgttaattaa tgacccgctg ttaacgcaat atattaattc gctggggatg
    25261 cgtctggttt cgcatgccaa ttcggttaag acaccgtttc atttttttct gatcaacaac
    25321 gacgaaatta acgcctttgc tttctttggc ggcaacgtgg tgctgcactc tgccctgttc
    25381 cgttattccg ataacgaaag tcaactggct tcagttatgg cgcacgaaat ctcccacgtc
    25441 acccaacgtc acctggcgcg agcgatggaa gatcaacaac gcagcgcgcc gctgacctgg
    25501 gtcggcgcgt taggttctat tttactggcg atggccagtc cgcaggcggg gatggcggcg
    25561 ctgaccggta cactggcggg aacgcgccag gggatgatca gtttcaccca gcaaaatgaa
    25621 caggaagcgg accgcattgg tattcaggtg ctgcaacgct cgggattcga tccacaggcg
    25681 atgccgacct tcctggaaaa attgctcgat caggcgcgtt actcctcgcg cccgccggaa
    25741 attttattga ctcacccgtt gccggaaagt cgtctggcag atgcccgcaa ccgtgctaat
    25801 cagatgcgcc cgatggtggt gcagtcgtcg gaagatttct atctggcgaa agcgcgcaca
    25861 ctggggatgt ataattccgg acgtaaccag ctcaccagtg atttgctgga tgaatgggcg
    25921 aaaggaaacg ttcgtcagca acgagcggcg caatatggtc gtgctttaca ggcgatggaa
    25981 gccaataaat acgacgaggc gcgtaaaacg ctgcaaccgt tactggcggc agaacctggc
    26041 aacgcatggt atctcgatct ggctaccgat atcgatcttg ggcaaaacaa agccaatgag
    26101 gcaatcaatc gcctgaaaaa tgcccgcgat ttacgcacca atccggtgtt gcagctcaac
    26161 ctggcgaacg cttatctgca aggcggtcaa ccacaagaag cggccaatat tctgaatcgc
    26221 tacaccttta ataataaaga tgacagcaac ggctgggatt tgctggcaca ggcggaagcc
    26281 gcgctaaata accgcgatca ggagctggct gcgcgagcag aaggttatgc gctcgccgga
    26341 cgactcgatc aggccatttc gctgttgagt agcgccagtt cgcaggtgaa attaggcagc
    26401 ctgcaacaag cgcgttacga tgcgcgcatc gaccagttgc gccagctgca gaaacgcttt
    26461 aagccttata ccaagatgta attcgcatta aaggaagaac aatgaccaaa caggtaaaaa
    26521 tctaccataa cccacgctgt tcaaagagcc gggaaacgct gaacctgctg aaagaaaacg
    26581 gcgtggagcc agaagtggtg ctctaccttg agacacccgc cgatgcggca acgctgcgcg
    26641 atttgctgaa aatgctgggg atgaatagcg cccgtgaatt gatgcgccag aaagaggatc
    26701 tttataaaga actaaacctg gctgacagtt cgcttagcga agaggcgctg attcaggcaa
    26761 tggtggataa tccgaagctg atggagcgcc cgattatggt cgcgaatggc aaagcgcgga
    26821 ttggtcgtcc accggagcag gtactggaaa tcgtgggtta agatgtgctc catcgctagt
    26881 tgaagcacat tgccggatgc gacgcttgcc acgtcttatc cggcctacga atactatgga
    26941 ttaacttcgc gtaggtcagg cgttcgcgcc gcatccgaca ataaacacct tatctacaac
    27001 ttcagaattt ctttcacaaa cggaatggtc agcttacgtt gcgcggtaat cgacgcacga
    27061 tccaactgat ccaacgtcat aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag
    27121 aaacgcccca catcttccgg cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc
    27181 tgcaacttat cttcatcaga aagtggctgc aatttgtaga tctgccccca gtcgagacgc
    27241 gacgcgagat ccggtaatcc cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac
    27301 aaccgtgttt tgcccgattc cagaattcga ttgtagagat cgaaaatcgc catctcccac
    27361 aactcatcgc ctgcaataca ctcaatgttg tcgatacaga ccagcgacaa atgctccata
    27421 ccgtcgagca cttccggaac aaaccaggtg cgtttatcca gcgggacata gcccaccgca
    27481 tcgccacgct gcgacaattc cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct
    27541 tcgcgtgccc agagatagat gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgcg
    27601 gccagtaaag aggagttatc ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga
    27661 taaagtggca aagagagctg tgccggtgtg ttcagagata cctcaaccag gatttcacaa
    27721 aatcgcgaga agtttaccac agaatcccat gatgtttgaa ccgggcagca acactgcccg
    27781 gtcggtacat tatttgtctg ttatatccgc gtcttctgcg tccagcacca cttcttcagg
    27841 gcgcagcaca ctgatcagtt tgaaaatcaa acttaagccg ataccgacga tggtcgccag
    27901 cgccatacct tttaactccg ccgcgccgat gtttaccttc gcgccactga cgccgatgat
    27961 caaaatcacg gaagtcagga tcaggttctg tgctttgttg taatccactt tcgattcgat
    28021 caaaacacga ataccggaag caccaatgac accataaagc agcagcgaaa cgccgcccat
    28081 caccggcaat gggatcatct ggatagcggc agccagttta ccgacgcagg aaagcaggat
    28141 agcgaaaatc gccgccccgc cgataaccca ggtactgtaa acacgggtga tcgccatcac
    28201 gccaatgttt tctccgtaag tggtatttgg cgtagagcca aagaagccgg agatcacggt
    28261 cgacaggccg ttagcaaaca tcgaacggtg cagacctgga tcgcgcagca gatctttttt
    28321 gacgatatta gccgttacta ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc
    28381 tggcagaata gtcagaatgg caaaccactc gaagcgcggc gtatagaggg ttggcagtgc
    28441 aaaccagtga gcattaataa tcggcgtggt atcgacaatt cccattgcga aagagagcgc
    28501 gtaccccacc agcacgccaa ttaaaatcgg gataattgcc aggaaaccac gaaacagcac
    28561 ggaacctaaa accgtgaccg ccagggtggt aatagagatg atgatggttt tggagtttgg
    28621 cgtttgccct tcagccggga gtaaacccgc cataccggca gctacgcccg ccagctccag
    28681 accgatgacg gcaacgattg cgcccattgc cgcaggtgga aacagcacgt ccaaccagcc
    28741 ggtccccgct ttcttcacga taaaagaaac caggcagaac agcacgccgc acataataaa
    28801 gccgcccagc gcgacttcat accctaacgg caacagtaac aataccggtg aaataaaggc
    28861 aaagctggaa ccaagataag ccggaatttt ccctttacag atgaagagat acagcagcgt
    28921 tccaataccg ttaaataaca gtacagtcgc cgggttaata tgaaataaga cgggcaccag
    28981 gacggttgca ccaaacatgg cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag
    29041 tggcggtctt tcactcaccc cgatagcacg gcgcgtcata gtattatcct ctgtattatg
    29101 tgttataggc gctttactca aaaaaaagcc gactcttaaa gtcggcttta attattttta
    29161 ttctttattt cgtaccaaag attttgtcac cggcatcgcc gaggcccgga ataatgtatc
    29221 cgtgctcgtt cagtccctgg tcaatcgatg cagtatacag ttcgacgtcc gggtgcgctt
    29281 tttccagcgc agcgatacct tctggcgcag cgaccagcac cagaactttg atgctgctgc
    29341 agcccgcttt tttcagcagg tcgatggtcg cgataacgga accaccggtt gccagcattg
    29401 ggtcaacgat cagcgccata cgctcatcga tgttagaaac cagtttctgg aagtacggta
    29461 ccggctccag cgtttcttca ttacggtaca taccgacaac gctgatgcgc gcgctcggaa
    29521 cgttttccag cacaccgtcc atcataccaa gacccgcacg cagaattggc acaacggtaa
    29581 ttttcttacc tttgatctgg tcgatttcta ccgggccgtt ccagccttcg atagttactt
    29641 tttccgtttc gaggtcggcg gtcgcttcgt aagtcagcag gctacccact tcggaagcga
    29701 gttcgcgaaa gcgcttggtg ctgatatctt gctcacgcat cagtcccagc ttgtgtttga
    29761 cgagtgggtg tttgacttcc acgatcttca tactctttct cctttgaggg gcagccacaa
    29821 aaaaatcgac ggattatacc tcctttcttc agggcggcaa tattattttc gttgacttta
    29881 gtcaaaatga taacagtttg agataaagtt attttatatt caaatggtta tgaaagaaga
    29941 ttattccatc cgaaaactaa cctttgccct ggcacaagtc ttctttcgcc gcgcgcccgg
    30001 ggaaaagacg tgcaaaaagg ttgtgtaaaa cagtctcgca aacgtttgct ttccctgtta
    30061 gaattgcgcc gaattttatt tttctaccgc aagtaacgcg tggggaccca agcagtgacc
    30121 gataaaacct ctcttagcta caaagatgcc ggtgttgata ttgacgcggg taatgctctg
    30181 gttggaagaa tcaaaggcgt agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg
    30241 ggcggcttcg gtgcgctgtg tgcattgccg caaaaatatc gtgaacccgt gctggtttcc
    30301 ggcactgacg gcgtaggtac caagctgcgt ctggcaatgg acttaaaacg tcacgacacc
    30361 attggtattg atctggtcgc catgtgcgtt aatgacctgg tggtgcaagg tgcggaaccg
    30421 ctgtttttcc tcgactatta cgcaaccgga aaactggatg ttgataccgc ttcagcggtg
    30481 atcagcggca tcgcggaagg ttgtctgcaa tcgggctgtt ctctggtggg tggcgaaacg
    30541 gcagaaatgc cggggatgta tcacggtgaa gattacgatg tcgcgggttt ctgcgtgggc
    30601 gtggtagaaa aatcagaaat catcgacggc tctaaagtca gcgacggcga tgtgctgatt
    30661 gcactcggtt ccagcggtcc gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa
    30721 gtcagcggtt gtgatccgca aaccaccgaa cttgatggta agccattagc cgatcatctg
    30781 ctggcaccga cccgcattta cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg
    30841 catgccattg cgcacctgac cggcggcggc ttctgggaaa acattccgcg cgtattgcca
    30901 gataataccc aggcagtgat tgatgaatct tcctggcagt ggccggaagt gttcaactgg
    30961 ctgcaaacgg caggtaacgt tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc
    31021 gggatgatta tcgccctgcc tgctccggaa gtggacaaag ccctcgccct gctaaatgcc
    31081 aacggtgaaa acgcgtggaa aatcggtatc atcaaagcct ctgattccga acaacgcgtg
    31141 gttatcgaat aatgaatatt gtggtgctta tttccggcaa cggaagtaat ttacaggcaa
    31201 ttattgacgc ctgtaaaacc aacaaaatta aaggcaccgt acgggcagtt ttcagcaata
    31261 aggccgacgc gttcggcctt gaacgcgccc gccaggcggg tattgcaacg catacgctca
    31321 tcgccagcgc gtttgacagt cgtgaagcct atgaccggga gttgattcat gaaatcgaca
    31381 tgtacgcacc cgatgtggtc gtgctggctg gttttatgcg cattctcagc ccggcgtttg
    31441 tctcccacta tgccgggcgt ttgctgaaca ttcacccttc tctgctgccg aaatatcccg
    31501 gattacacac ccatcgtcag gcgctggaaa atggcgatga agagcacggt acatcggtgc
    31561 atttcgtcac cgatgaactg gacggtggcc cggttatttt acaggcgaaa gtcccggtat
    31621 ttgctggtga tacggaagat gacatcaccg cccgcgtgca aacccaggaa cacgctattt
    31681 atccactggt gattagctgg tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt
    31741 ggctggatgg tcaacgtctg ccgccgcagg gctacgctgc cgacgagtaa tgcccccgta
    31801 gttaaagcgc cagctctgcc gctggcgttt ttcaattcac ctgtaaatcg caagctccag
    31861 cagttttttt cccccttttc tggcatagtt ggacatctgc caatattgct cgccataata
    31921 tccaggcagt gtcccgtgaa taaaacggag taaaagtggt aatgggtcag gaaaagctat
    31981 acatcgaaaa agagctcagt tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg
    32041 acaaatctaa cccgctgatt gaaaggatgc gtttcctggg gatctattcc aataaccttg
    32101 atgagttcta taaagtccgc ttcgctgaac tgaagcgacg catcattatt agcgaagaac
    32161 aaggctccaa cgctcattcc cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag
    32221 ccgatcagga attcgacggc ctctacaacg agctattgct ggagatggcg cgcaaccaga
    32281 tcttcctgat taatgaacgc cagctctccg tcaatcaaca aaactggctg cgtcattatt
    32341 ttaagcagta tctgcgtcag cacattacgc cgattttaat caatcctgac actgacttag
    32401 tgcagttcct gaaagatgat tacacctatc tggcggtgga aattatccgt agcgatagca
    32461 tccgttacgc gctgctggag atcccatcag ataaagtgcc gcgctttgtg aatttaccgc
    32521 cagaagcgcc gcgtcgacgc aagccgatga ttcttctgga taacattctg cgttactgcc
    32581 ttgatgatat tttcaaaggc ttctttgatt atgacgcgct gaatgcctat tcaatgaaga
    32641 tgacccgcga tgccgaatac gatttagtgc atgagatgga agccagcctg atggagttga
    32701 tgtcttccag tctcaagcag cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg
    32761 atatgcccaa tgcgctggtt gaagtgttac gcgaaaaact gactatttcc cgctacgact
    32821 ccatcgtccc cggcggtcgt tatcataatt ttaaagactt tattaatttc cccaatgtcg
    32881 gcaaagccaa tctggtgaac aaaccactgc cgcgtttacg ccatatttgg tttgataaag
    32941 cccagttccg caatggtttt gatgccattc gcgaacgcga tgtgttgctc tattatcctt
    33001 atcacacctt tgagcatgtg ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac
    33061 tggcgattaa aattaacatt taccgcgtgg cgaaagattc acgcatcatc gactcgatga
    33121 tccacgccgc acataacggt aagaaagtca ctgtggtggt tgagttacag gcgcgtttcg
    33181 acgaagaagc caacattcac tgggcgaagc gcctgaccga agcaggcgtg cacgttatct
    33241 tctctgcgcc ggggctgaaa attcacgcca aactgttcct gatttcacgt aaagaaaacg
    33301 gtgaagtggt ccgttacgca cacatcggga ccgggaactt taacgaaaaa accgcgcgtc
    33361 tttatactga ctattcgttg ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg
    33421 tatttaactt tattgaaaac ccataccgcc cggtgacatt tgattattta atggtgtcac
    33481 cgcaaaactc tcgccgtctg ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc
    33541 aagggctgcc cagtggtatc accctgaagc taaataacct tgtcgataaa ggcctggttg
    33601 atcgtctgta tgcggcctcc agctccggcg taccggttaa tctgctggtt cgcggaatgt
    33661 gttcgctgat ccccaatctg gaaggcatta gcgacaacat tcgtgccatc agtattgttg
    33721 accgttacct tgaacatgac cgggtttata tttttgaaaa tggcggcgat aaaaaggtct
    33781 acctttcttc cgccgactgg atgacgcgca atattgatta tcgtattgaa gtggcgacac
    33841 cgctgctcga tccgcgcctg aagcagcggg tgctggacat catcgacata ttgttcagcg
    33901 atacggtcaa agcacgttat atcgataaag aactcagtaa tcgctacgtt ccccgcggca
    33961 atcgccgcaa agtacgggcg cagttggcga tttacgacta catcaaatca ctcgaacaac
    34021 ctgaataacc ctatgccaat acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc
    34081 gatcttggtt caaacagttt tcacatggtc atagcccgtg tggtagctgg tgccatgcag
    34141 attattggcc gcctgaaaca gcgggtgcat ctggcggacg gcctggggcc agataatatg
    34201 ttgagtgaag aggcaatgac gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta
    34261 caagggtttt ctcctgccag cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg
    34321 aacgccactg actttctgaa acgcgcggaa aaggtcattc cctacccgat tgaaattatt
    34381 tccggtaatg aagaagcccg tctgattttt atgggcgtgg aacataccca accggaaaaa
    34441 ggtcgcaaac tggttattga tattggcggc ggatctacgg aactggtgat tggtgaaaat
    34501 ttcgaaccta ttctcgttga aagccgccgg atgggttgtg tcagctttgc ccagctttat
    34561 tttcctggcg gggtcatcaa taaagagaat tttcagcgcg ctcgcatggc ggcagcacaa
    34621 aaactggaaa ctttaacctg gcaattccgt attcagggct ggaacgttgc aatgggcgct
    34681 tccggtacca taaaagccgc ccatgaagtg ttaatggaaa tgggcgagaa agacgggata
    34741 attaccccgg aacgtctgga aaaactggta aaagaaattt tacgtcaccg taatttcgca
    34801 tcgctgagtt taccgggtct ttccgaagag cggaaaacag tcttcgttcc tggactggcg
    34861 attttatgtg gtgtgtttga tgctttagcc atccgtgaac tgcgcctttc tgacggggcg
    34921 ttacgcgaag gcgtactgta tgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt
    34981 cgcaccgcca gcagcctcgc caaccagtat cacatagaca gcgaacaggc ccgacgagtg
    35041 ctggatacca ctatgcaaat gtacgaacag tggcgggaac agcaaccgaa gctggcgcat
    35101 ccgcaactgg aggcgctact gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc
    35161 aaccacagcg gtttgcatcg ccactccgct tatattctgc aaaacagtga cttgccgggt
    35221 tttaatcagg aacagcagct gatgatggcg acactggtgc gctatcaccg taaagcgatt
    35281 aagctcgacg atctgccgcg ctttaccttg tttaagaaga aacagttcct gccactgata
    35341 cagctattgc gccttggcgt attactaaac aatcaacgtc aggcaaccac cacaccgcca
    35401 acattgacac tgattactga tgacagtcac tggacactgc gtttcccgca tgactggttt
    35461 agtcagaatg cgctggtact gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg
    35521 gctggctggc ggttgaaaat tgaagaagaa agtaccccag aaatcgccgc ttaatgcttt
    35581 gcgggccgac gagaaatgtc ggcccgcatt attcaggcac tttcgcgaat gggttcgatt
    35641 tcattcagcg tatcaattaa cggttgcggc ttaccaataa gataaccctg catataatcg
    35701 atccccaaag agagcaccgc ctcgcggatc tcttcgtttt caacgtactc tgccactacc
    35761 cgcattttct tcattcgcgc cagatggcaa atcgacgcca caatctgata atccagacta
    35821 tttgacacaa tattgcggat aaagctgccg tcaattttaa gcagatcggc attcacattt
    35881 ttaagccgtg catagctggc gtagccggtg ccgaaatcat caatcgcaat ctggcagcct
    35941 aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa cattggtcag agcattactt
    36001 tcggtgactt caaaaataag ttgccacgct tcaatctgat atttagccag caactgactg
    36061 acttcaacag gaaaacgagc ctgacatacc gaggttggag acagattaat agcaaaacgg
    36121 tgagcgggca tcttcgctct gttttcagcc ataaattgca gcgtatgctc aatgacccac
    36181 atgtcgatac tcgacgataa accaaattcg tgcgcgaccg gcaagaagct accggggccg
    36241 atcagttcat cattctcacc tttcatgcgc agaagaattt catggtaaac atcaccacgc
    36301 ataccggtaa tcggctgggc catcaggaaa aaatggttgt gttccagcgc ctgctgtagt
    36361 cgattcatca tcgcgacttt atctttcaat tcgcgttgca aatacattgc cccgcaacgc
    36421 tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc
    36481 agcagcaggt agatatgatt cactggcgag cgcacatagc agtaactgac gccaatctgc
    36541 ggttgcatcg gcatcccatc ccaaaagaaa cgaaattgct tgagatgact atccagtgcg
    36601 gtaatgcgct cctggtgcga ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc
    36661 tgataaacat cttcacccgg ttccagcaag ggtgacagcc agtgagaaag tttttgcttg
    36721 ta
//
LOCUS       DBBBSB010000027        35443 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.027, whole genome shotgun sequence.
ACCESSION   DBBBSB010000027 DBBBSB010000000
VERSION     DBBBSB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.027"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="ACIUS0_001362"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="ACIUS0_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGB0219178.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACIUS0_001363"
     CDS             complement(204..275)
                     /gene="ysaE"
                     /locus_tag="ACIUS0_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HGB0219179.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACIUS0_001364"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACIUS0_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGB0219180.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="ACIUS0_001365"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="ACIUS0_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="ACIUS0_001366"
     CDS             1631..2341
                     /locus_tag="ACIUS0_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGB0219181.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACIUS0_001367"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACIUS0_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGB0219182.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4129)
                     /locus_tag="ACIUS0_001368"
                     /pseudo
     CDS             complement(3469..4129)
                     /locus_tag="ACIUS0_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            4282..6480
                     /locus_tag="ACIUS0_001369"
     CDS             4282..6480
                     /locus_tag="ACIUS0_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HGB0219183.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6449..6889)
                     /locus_tag="ACIUS0_001370"
     CDS             complement(6449..6889)
                     /locus_tag="ACIUS0_001370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGB0219184.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACIUS0_001371"
     CDS             complement(6886..7449)
                     /gene="tag"
                     /locus_tag="ACIUS0_001371"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGB0219185.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7607..8305
                     /locus_tag="ACIUS0_001372"
     CDS             7607..8305
                     /locus_tag="ACIUS0_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGB0219186.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8534..9742
                     /locus_tag="ACIUS0_001373"
     CDS             8534..9742
                     /locus_tag="ACIUS0_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219187.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10066..11757
                     /gene="eptB"
                     /locus_tag="ACIUS0_001374"
     CDS             10066..11757
                     /gene="eptB"
                     /locus_tag="ACIUS0_001374"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGB0219188.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11849..11925
                     /locus_tag="ACIUS0_001375"
     tRNA            11849..11925
                     /locus_tag="ACIUS0_001375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11883..11885,aa:Pro,seq:cgg)
     gene            12669..14276
                     /gene="dppA"
                     /locus_tag="ACIUS0_001376"
     CDS             12669..14276
                     /gene="dppA"
                     /locus_tag="ACIUS0_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGB0219189.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14584..15603
                     /gene="dppB"
                     /locus_tag="ACIUS0_001377"
     CDS             14584..15603
                     /gene="dppB"
                     /locus_tag="ACIUS0_001377"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGB0219190.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15613..16515
                     /gene="dppC"
                     /locus_tag="ACIUS0_001378"
     CDS             15613..16515
                     /gene="dppC"
                     /locus_tag="ACIUS0_001378"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGB0219191.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16526..17509
                     /gene="dppD"
                     /locus_tag="ACIUS0_001379"
     CDS             16526..17509
                     /gene="dppD"
                     /locus_tag="ACIUS0_001379"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGB0219192.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17506..18510
                     /gene="dppF"
                     /locus_tag="ACIUS0_001380"
     CDS             17506..18510
                     /gene="dppF"
                     /locus_tag="ACIUS0_001380"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGB0219193.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18540..19811)
                     /locus_tag="ACIUS0_001381"
     CDS             complement(18540..19811)
                     /locus_tag="ACIUS0_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGB0219194.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20287..20394
                     /locus_tag="ACIUS0_001382"
     CDS             20287..20394
                     /locus_tag="ACIUS0_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGB0219195.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20770..20877
                     /gene="ldrD"
                     /locus_tag="ACIUS0_001383"
     CDS             20770..20877
                     /gene="ldrD"
                     /locus_tag="ACIUS0_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HGB0219196.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21253..21360
                     /locus_tag="ACIUS0_001384"
     CDS             21253..21360
                     /locus_tag="ACIUS0_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGB0219197.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21736..21843
                     /locus_tag="ACIUS0_001385"
     CDS             21736..21843
                     /locus_tag="ACIUS0_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGB0219198.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACIUS0_001386"
     CDS             complement(21930..23609)
                     /gene="bcsG"
                     /locus_tag="ACIUS0_001386"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGB0219199.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACIUS0_001387"
     CDS             complement(23606..23797)
                     /gene="bcsF"
                     /locus_tag="ACIUS0_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGB0219200.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACIUS0_001388"
     CDS             complement(23794..25365)
                     /gene="bcsE"
                     /locus_tag="ACIUS0_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGB0219201.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25638..25826
                     /gene="bcsR"
                     /locus_tag="ACIUS0_001389"
     CDS             25638..25826
                     /gene="bcsR"
                     /locus_tag="ACIUS0_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGB0219202.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACIUS0_001390"
     CDS             25838..26590
                     /gene="bcsQ"
                     /locus_tag="ACIUS0_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGB0219203.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26587..29204
                     /gene="bcsA"
                     /locus_tag="ACIUS0_001391"
                     /pseudo
     CDS             26587..29204
                     /gene="bcsA"
                     /locus_tag="ACIUS0_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29215..31554
                     /gene="bcsB"
                     /locus_tag="ACIUS0_001392"
     CDS             29215..31554
                     /gene="bcsB"
                     /locus_tag="ACIUS0_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGB0219204.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACIUS0_001393"
     CDS             31561..32667
                     /gene="bcsZ"
                     /locus_tag="ACIUS0_001393"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGB0219205.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32862..33251
                     /locus_tag="ACIUS0_001394"
     CDS             32862..33251
                     /locus_tag="ACIUS0_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HGB0219206.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            33333..35321
                     /gene="hmsP"
                     /locus_tag="ACIUS0_001395"
     CDS             33333..35321
                     /gene="hmsP"
                     /locus_tag="ACIUS0_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGB0219207.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         8449 a         8861 c         9513 g         8620 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggcaggc
     4561 ggttataccg ggcatctggg ggattattcg accggtgcgg cacaggcgat catgccgtat
     4621 gtcgtaggtg gcagcgaagt ttatcaacag caaaccagtt ggccgctggt gctggaacat
     4681 agcgatgtcg tggtgttgtg gagtgctaac ccactcaata cgctgaaaat tgcgtggaat
     4741 gcatccgatg agcaggggct ttcttacttt tccgcactgc gtgacagtgg gaaaaagctg
     4801 atctgcattg atccaatgcg atcggaaacc gtcgatttct ttggcgataa aatggagtgg
     4861 gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg
     4921 gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca ccacaggtta tgccgtcttc
     4981 gcctcttatt tgctgggcga gagtgacgga atagcgaaaa acgccgaatg ggcggcagag
     5041 atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg ctattttcca ccaaaatacc
     5101 accatgctga tggcaggctg gggaatgcag cgccaacagt ttggtgagca aaaacactgg
     5161 atgatcgtca cgctggcagc aatgttgggg caaatcggca cacccggcgg cggttttggt
     5221 ctttcttacc attttgccaa tggtggtgac cccacgcggc gttctgcggt gctctcttcc
     5281 atgcagggca gcttgccggg tggctgcgat gcggtggata aaatccctgt tgcccgcatt
     5341 gttgaagcac tggaaaaccc tggtggcgca tatcaacaca acggtatgaa ccgacatttc
     5401 ccggatattc gttttatctg gtgggcgggc ggtgccaact ttactcatca tcaggatacc
     5461 aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg tgatctctga atgcttctgg
     5521 acggcggcgg cgaaacacgc ggatattgtt ctgcctgcga ctacctcttt tgagcgtaat
     5581 gatctcacca tgaccggtga ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg
     5641 ccgccacgct atgaagcgcg taatgatttt gatgtttttg ccgagttgag tgaacgttgg
     5701 gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg
     5761 ttttataacg ttgcccggca gcgcggggca agccagcagg ttgaattgcc gccatttgct
     5821 gagttctggc aagccaacca gttaattgag atgccggaaa acccggacag cgagcggttt
     5881 attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc
     5941 aagattgaaa tcttctcaca gcgtattgcc gattacggtt acccggattg ccctgggcat
     6001 ccaatgtggc tggagccgga cgaatggcag ggcaatgccg aaccagaaca gttgcaggta
     6061 ctttctgccc atccggcgca ccgcctgcac agccagctga attacagttc tctgcgcgaa
     6121 ttgtacgcgg tagcaaaccg tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc
     6181 ggcataaccg aaggggatat ggtgcgggtg tggaactccc gtgggcagat ccttgccgga
     6241 gcggtcatta gcgagggaat taaacctggc gtgatttgca ttcacgaagg ggcatggccg
     6301 gatctggatt taaccgctga cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa
     6361 gatctcccca gctcgcggct ggggaatggc tgtgcgggta atacgacgct ggcatggctg
     6421 gaaaaataca acggtccgga actgacactt acagcgtttg aaccaccggc cagctcataa
     6481 tccaggtggg tagttgggtt tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg
     6541 cacggtaaaa atctatcgcc ggttgatttt tttgataaac ctccagcatc agatggggat
     6601 agcgctgctg cacatactgc atcagcgcct taccaatacc gcgtctgacg gccttcggtg
     6661 cgacaaacat tgctgccaga aatcggcctt ccataatgct gacaaaaccg agaagcttac
     6721 cgtcctcttc ccagacccag ttttgcgcgt tggcaagata ggcatcccgc accagcggaa
     6781 tgcagtcacg ccagtaattc gcttttataa agggatgccc ccaggttgta ctttccagcc
     6841 acagttcgag gatcgcgggg agttctgaac gttgcgcttc ccgaatcatg gtttatttcc
     6901 cagatagcaa cagcagccaa ccacatgatc attcaccagc ccacatgcct gcataaagga
     6961 gtaacagatt gtggtgccga caaacttaaa accacgtttt ttcagtgcct tagatagggc
     7021 gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg
     7081 ctgatgattt acaaacgacc agacaaagtc gggaaacggt tcgccgttct gttccatttg
     7141 caggtacgcc cgcgcattac caataattgc ctgaattttc cctcgatggc ggataatccc
     7201 ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt gctgcgacct tcaccggatc
     7261 gaactgatga aagtaggcgc gatagttttc gcgttttttg aggacggtga tccacgataa
     7321 cccagcctgc tgcccttcaa agcagatcat ttcgaacagt tttttactgt cagtttcagg
     7381 cacgccccac tcattatcat ggtaggcaat ataaagcggg ccctgactca cccagccgca
     7441 acgttccata ctttccctcg ctatgtaccc gatcgtaaaa atttcagccg cttttcttga
     7501 cgcatcggtg aaaaagtcaa tatttaatac agggctacta tgcccgatat ccttcataca
     7561 atgacaataa tattgccgaa ttcggctctt ccctggagtc gtgttaatga tcataaaaaa
     7621 aagcggtggt cgctggcagc taagcctgct ggcgagcgtg gtaatcagtg ccttttttct
     7681 caacacagct tacgcctggc aacaagaata tatcgttgat acgcaacccg gacattccac
     7741 agagcgttac acctgggata gtgatcatca acctgattac aacgatattt tgtcgcaacg
     7801 tattcaaagt agccaaaggg cgctgggact ggaagtcaat ctggcggaag aaactcctgt
     7861 ggatgtgacc agcagtatga gtatgggctg gaattttcct ttatatgaac aggttacaac
     7921 cggcccggtc gcggcattac attacgatgg cacaaccacc tcgatgtata acgagtttgg
     7981 cgacagtact accacgctga ccgatccgtt atggcatgcc agcgtgagta gcttaggctg
     8041 gcgtgttgac tcccggcttg gcgatctccg accctgggcg caaatcagct ataaccagca
     8101 atttggcgag aatatctgga aggcgcaatc aggcctgagc cggatgacgg cgacaaacca
     8161 gaacggcaac tggctggatg tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc
     8221 cgcctatgct gcgctaactc aggcagaaaa taccactaat aatagcgact atctgtatac
     8281 gatgggggtt agcgccagat tttaacgtaa cagtcacaat tgaaaccatt gaataacaac
     8341 agttgtggcg atagtgggtg ctaacttacc aaataataaa tttggtgaat aattgtcgcg
     8401 tcattcattc ctgaactaag gcatttcatt ccgttctgat ggcatttcat gccgtttttc
     8461 cccaagcata aaatgcactt cgttatggtt gtcggcagag atttttcctt tttattactg
     8521 caggaatact gccatgacac cttcaaatta tcagcgtacc cgctggctga cactcatcgg
     8581 tactatcatt acccagtttg cgctggggtc ggtttatacc cggagcctgt ttaatggcgc
     8641 gctttctgcc aagctggatg cgccggtaag ccaggtcgct ttctctttcg gcttgttaag
     8701 tctggggctg gcaatttcgt cttctgttgc gggcaaattg caggaacgtt ttggcgttaa
     8761 acgcgtcacc gtggcttccg gcattttgct gggattaggc ttcttcctga ccgcgcattc
     8821 caacaacctg atgatgctgt ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc
     8881 gggttacctg ctgacgctct ctaactgcgt gaagtggttc ccggagcgta aaggtctgat
     8941 ctccgcgttc gctatcggtt cttatggtct gggtagcctg ggtttcaaat ttatcgacac
     9001 gcagctgctc gaaacggtgg gtctggaaaa aacctttgtg atttggggag cgattgcgct
     9061 ggtgatgatt gttttcggcg caacgttaat gaaagacgca ccaaaacagg aagtgaaaac
     9121 cagcaatggt gtggtggaga aagattacac gctggcagag tcgatgcgta aaccgcagta
     9181 ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc ggcctgtacg tgattggggt
     9241 agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt
     9301 aacggttatt tccatcgcta acctttcagg tcgtctggtg ctgggcattc tgtctgacaa
     9361 aattgcccgt atccgtgtta tcaccattgg tcaggtgatt tcgctggtgg gtatggcggc
     9421 cctgctgttt gcaccattaa atgcagtgac gttctttgca gcgattgcct gcgtggcatt
     9481 taactttggc ggcactatta ccgtctttcc atcactggtc agtgagttct tcggcctcaa
     9541 taacctggcg aaaaactacg gtgtgattta cctcggtttc ggtatcggta gcatttttgg
     9601 ttcgattatc gcctcactgt ttggcggttt ctatgtgacc ttctacgtga ttttcgccct
     9661 gctgattctg tcattggcgc tttctacgac gattcgtcag ccagagcaga aaatgttgcg
     9721 tgaggcgcat ggctcccttt aatgctcagc cgcgagctac tgggcttcag cttccgtgtg
     9781 catcaaacgc atcaacgaaa tgtataaaca atgactcgta aaatttgggc cagttaactg
     9841 gcccttttta ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat
     9901 ttgtgcttca tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg
     9961 cgcgtgtaga ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag
    10021 ccgctaagca gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa
    10081 tcgattacac agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat
    10141 ggcgcggttt tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc
    10201 gtttctgctg ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt
    10261 ctatcgctgt ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc
    10321 gcaggtgcca gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct
    10381 tccgtcatga ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc
    10441 tggttaatcg ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac
    10501 accttgctcc gacaactgcg aaccccaggg cagcgtattc gcagcctggt ggtcgtcgta
    10561 ctggcgggta ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg
    10621 gagagggcga ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg
    10681 ccatcaaact ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc
    10741 gataataatt cattgcttaa tccggcgaag aagtttacat accaggctcc acaaaacgtt
    10801 gatgatactt atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt
    10861 ttcggctatg agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc
    10921 cgtggttact cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag
    10981 gggggcgcgg aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg
    11041 aagcagttag gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc
    11101 aacacgatgg cagacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt
    11161 ggcaagccgg tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac
    11221 ccggatggta agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag
    11281 cgttatccgc gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt
    11341 accaaagcgc agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc
    11401 tccagcgtga ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac
    11461 ggtgagtcaa ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg
    11521 gagcagttcc gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc
    11581 aatgcgcagg cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac
    11641 gtagagctgt acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt
    11701 aacgaaaaca acaactggtg tcacatcccg cagacaaaag tggcagcggc taactaagcg
    11761 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
    11821 atctgcgcag taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg
    11881 ttcgggacga aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg
    11941 ctcaacgagc aggttttttt gcatttgtag ctcatgagga tgagaacctc cgggggcagg
    12001 gaggctcgac tcgacgtagg cctgataaga cgcgccagcg tcgcatcagg caacggcacc
    12061 gcacttattg tcggatgcgg cgcgaccgcc ttattcgacc aacacaccac acattttcct
    12121 gccaactttg cgccccatat ctcactatcc attaacggat ttgtgacaga aaccattgtc
    12181 tttttttata tttgcttaaa tcttttttgc gttacttatg gctggcgctg gcattttccg
    12241 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
    12301 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
    12361 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
    12421 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
    12481 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
    12541 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
    12601 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
    12661 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
    12721 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
    12781 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
    12841 tcccgcttta taaccgcctg gttgaattta aaatcggcac caccgaagtg atcccgggcc
    12901 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
    12961 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg cgaaatgaac gccgatgacg
    13021 tggtgttctc gttcgatcgt cagaaaaacg cgcaaaaccc gtaccataaa gtttctggcg
    13081 gcagctacga atacttcgaa ggtatgggct tgccggagct gatcagcgaa gtgaaaaagg
    13141 tggacgataa caccgttcag tttgtgctga ctcgaccgga agcgccgttc ctcgctgacc
    13201 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgacgcgatg atgaaagccg
    13261 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
    13321 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
    13381 agatcgatac gctggtcttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
    13441 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
    13501 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcgtata
    13561 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca agcactaacc tacgcggtga
    13621 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
    13681 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
    13741 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
    13801 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
    13861 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    13921 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    13981 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    14041 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    14101 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcagg
    14161 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    14221 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    14281 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    14341 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    14401 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    14461 cctctgcgtg cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa
    14521 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg agtcactata gagaatccgg
    14581 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    14641 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    14701 ggcgaacgtg ggatctcccc tgagcgtcac gtgcagctgc tggctgaact cggcttagat
    14761 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    14821 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    14881 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    14941 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    15001 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    15061 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    15121 ccgttaaccg gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc
    15181 gatgccgtcg cccatatgat cttgcccgcc attgtgctgg gtactattcc gctagcggtc
    15241 attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc
    15301 gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    15361 atgctgccgg tagtgaccgt tatcggcctc caggtgggaa cattgctggc gggggcgatt
    15421 ctgaccgaaa ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcactgcaa
    15481 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    15541 gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    15601 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    15661 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg
    15721 gtttacgtcg ccatcgtgct gttcatcgcg atctttgcca actggattgc gccttataac
    15781 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagt
    15841 atggcacact tgctgggcac cgatgacgtc ggtcgtgatg tgctgtcgcg gctgatgtac
    15901 ggcgcgcgtt tatcgctgct ggttggctgt ctggtggtgg tgttgtcgct gattatgggc
    15961 gttattctcg gcctgatcgc cggttacttc ggcggcctgg ttgataacat tatcatgcgc
    16021 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    16081 ttcggcccgt cgattggtaa cgccgcgctg gcgctgacct tcgttgcctt gccgcactat
    16141 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt caccgcctct
    16201 cgcgtggcag gtgccggggc gatgcgccag atgtttatta acatcttccc gaactgcctt
    16261 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct
    16321 cttggtttcc tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc
    16381 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttccctgg tctggcgatc
    16441 ctgttgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    16501 aaactaaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    16561 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    16621 gggcgaagtg gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc
    16681 gattatgggg ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg
    16741 ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    16801 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    16861 tatggaagcg attaaagtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat
    16921 tgacctgctg aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca
    16981 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    17041 aaaactgctg attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat
    17101 catcgaacta ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    17161 tgacctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggtcaggt
    17221 agtagaaacg ggcgatgcac acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    17281 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    17341 tgttgttccc ggcaagtatg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    17401 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca
    17461 gtccaaatgc cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag
    17521 gccacctcgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    17581 aaaggcatgt tcgtgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt
    17641 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    17701 cggttgctga cgatgatcga aacgcccacc ggcggcgagc tgtattatca ggggcaggat
    17761 ctgctcaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    17821 cagaatccct acggttcgct gaatccgcgt aagaaagttg ggcaaattct cgaagagccg
    17881 ctgcttatta acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    17941 gcaaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcacatgtt ttccggcgga
    18001 caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    18061 gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc aggtgctgaa tctgatgatg
    18121 gatttgcagc aggagttggg gctttcttat gtctttatct cccacgacct gtcggttgtg
    18181 gagcacattg ctgatgaagt gatggtgatg tacctaggcc gctgcgtgga gaaaggaacg
    18241 aaggaccaaa tcttcaataa cccgcgccat ccgtacaccc aggcgctgct ctccgcgacg
    18301 ccacgcctga acccggacga tcgtcgtgaa cgcatcaaac tcaccggtga actgccaagc
    18361 ccgctgaatc ctccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    18421 tgcacccagt tgcagccgca gctaaaagac tacggaggtc aactggtagc ttgttttgct
    18481 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    18541 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaatcagc
    18601 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    18661 atgaggcacg acacaatgcc atataacggg cttcccaact gaaagaacac cagcactgat
    18721 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    18781 agcatgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    18841 cctttaatag cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    18901 atattcagca ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagt
    18961 gccagtgccg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    19021 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    19081 gtacgcagcg cgagccgggt tgccagtact ttatctgctt cccgtttacg ataggcaata
    19141 ttcattgggt taagtacctg aataaatacc gcagaaaaga agcaaaatgg aatggtaagc
    19201 aggacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    19261 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    19321 ccagaaatct taaacagtaa tcgttcacca ccagacgcaa tcgctatcag tacggcgaaa
    19381 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    19441 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    19501 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    19561 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    19621 actacccagg tgacaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    19681 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    19741 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    19801 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    19861 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    19921 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    19981 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    20041 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta gaatcagcgt
    20101 caagccggaa ggattccggt gaggcgcaat gttgcggggg cttaatccct ggtggcattg
    20161 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    20221 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    20281 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    20341 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    20401 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggggtgaaa
    20461 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    20521 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    20581 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    20641 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    20701 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    20761 ggcggctata tgacgttcgc agaactgggc atggccttct ggcatgattt agcggctccg
    20821 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    20881 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg
    20941 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    21001 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    21061 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    21121 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    21181 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    21241 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    21301 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    21361 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacta ggccgcaggg
    21421 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    21481 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    21541 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    21601 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    21661 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    21721 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    21781 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    21841 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    21901 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    21961 ttcaggcgaa cgtacggttt atcttggtat tgaataacta ctgcatttga gttctcggag
    22021 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    22081 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt
    22141 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    22201 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    22261 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    22321 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    22381 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    22441 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    22501 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    22561 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    22621 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    22681 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    22741 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    22801 ttcttgaaat caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    22861 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    22921 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    22981 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    23041 accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    23101 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    23161 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    23221 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    23281 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    23341 ctttccgggc caggcaacca gatgtcatgc cagaacaaag caaagccgat cggcagggca
    23401 atccagtggc gcaagcgatg caggctgtaa cgcggaatgg gcatcagcag aaacgcggca
    23461 aacaccaaat tgaggagcgg gtggaagtta agatatcccg cccacaacag gccaaactta
    23521 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    23581 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    23641 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc
    23701 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    23761 cgataataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    23821 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    23881 gaccggttta gaactttgcg ttaaaggcag cggcattccc cattgttctg gggcaagcag
    23941 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    24001 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    24061 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    24121 acggttaggg cgacatagcg tcagggcttg ctcaacgcgg atacccggta ccggacgtag
    24181 ggcaaccaga acgcctttac cgtgggcagg caagagaggg ttattcatca tgttgttgac
    24241 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    24301 cagcaaggta gtgatatctt ccggtacata gcgactaaac ttctgccctt gcacgctttc
    24361 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    24421 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    24481 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    24541 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    24601 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    24661 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    24721 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    24781 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    24841 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    24901 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    24961 attcatttta tccaaccatg agcgaagccg ctcggcagga atattttgcc atgcgttatt
    25021 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    25081 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    25141 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    25201 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    25261 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    25321 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    25381 gattatcagt ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc
    25441 agatttagca tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt
    25501 tttaactttt tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt
    25561 aaacaataaa ccggcgaaat gctcacggtt agaattgtct catgaatggt acggttattt
    25621 catagggatc aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag
    25681 gctatatctt ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg
    25741 attatgccga tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc
    25801 tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg gccgtactgg gattgcaggg
    25861 ggtgcgggga ggcgtgggga caacaaccat caccgccgca ttagcctggt cattacaaat
    25921 gttgggagaa aatgtcctgg tggtcgatgc ctgcccggac aacttgttgc gcctgtcatt
    25981 taatgttgat tttacccacc gtcagggctg ggccagagcg atgctggatg gccaggactg
    26041 gcgtgacgct gggttgcgct acacctcgca gctcgatttg ctgccttttg gtcagttatc
    26101 cattgaagaa caagaaaatc cacagcactg gcaaacccgg ctgagcgata tttgctccgg
    26161 cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt ttaatcgact taccgcgtga
    26221 tgcctcgcag ataacccacc agctgctgag tttgtgcgag cactcgctgg caatcgtcaa
    26281 tgtggatgcc aactgccata tccgactgca tcagcaagcg ctgccggatg gcgcacatat
    26341 tttgattaat gacttccgta ttggcagtca ggttcaggac gatatttacc agctttggtt
    26401 gcaaagccag cgccgattac tgccgatgct cattcatcgt gatgaagcga tggctgaatg
    26461 cctggcggct aagcaaccag taggtgaata tcgcagtgat gcgctggcgg ctgaagagat
    26521 actgacgctg gcgaactggt gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag
    26581 tgcatcatga gtatcctgac ccggtggttg cttatcccgc cggtcaacgc gcggcttatc
    26641 gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt
    26701 ttctggatga tcctggcctg gatttttatt ccgctggagc acccgcgctg gcagcgtatt
    26761 cgcgcagaac ataaaaacct gtatacgcat atcaacgcct cgcgtccgcg tccgctggac
    26821 ccggtccgtt atctcattca aacatgctgg ttattgatcg gtgcatcgcg caaagaaacg
    26881 ccgaaaccgc gcagcgggca ttttcaggtc tgcaaaatat tcgtggacgt taccatcaat
    26941 ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat gaaaaaaaag
    27001 agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc gtcaccttct
    27061 cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctggcg cagtttatct
    27121 tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg cgcttctcgg
    27181 cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg ttatatctgg tggcgttaca
    27241 cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt ctgctcttcg
    27301 ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta tggccgctga
    27361 atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg gccgtcagtg gatatctttg
    27421 tcccgactta caacgaagat ctcaatgtgg tgaaaaatac catttacgcc tcgctgggta
    27481 tcgactggcc gaaagacaaa ctgaacatct ggatcctcga tgacggcggc agggaagagt
    27541 ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgcaccact catgaacatg
    27601 cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc gtgtcgattt
    27661 tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc tggttcctga
    27721 aagagaaaca gctggcgatg atgcagactc cacaccactt cttctcgccg gacccgtttg
    27781 aacgcaacct gggacgtttt cgtaaaacgc cgaacgaagg cacgctgttc tatggtctgg
    27841 tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt gcggtgattc
    27901 gtcgtaagcc gctggatgaa attggcggca ttgctgtcga aaccgtgact gaagatgcgc
    27961 atacttctct gcgtttgcac cgtcgtggct atacctccgc gtatatgcgt attccgcagg
    28021 cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt cgctgggcgc
    28081 gcgggatggt ataaatcttc cgtctcgata acccgctcac cggtaaagga ctgaagtttg
    28141 ctcagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt ccacggctga
    28201 tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc tatgcgccag
    28261 cgttgatgat cgccctattc gtgctgccgc atatgatcca tgccagcctg accaactcga
    28321 agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg gtgctggcgt
    28381 ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc aaatttaacg
    28441 tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc tcgcggccct
    28501 acatcttcct tgttctactc aacctggtgg gcgttgcggt aggcatctgg cgctacttct
    28561 atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggtg ttctacaacc
    28621 tgattgttct tggcggcgca gttgcggtat cggtagaaag caaacaggta cgccgatcga
    28681 accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac ctcttctcgt
    28741 gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt caggcgcaga
    28801 ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa tacgtcttcc
    28861 cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg ccgctcacca
    28921 cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca tgggcgctct
    28981 ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt ctgaagctcg
    29041 gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag ggcatattcc
    29101 gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc ccggagcgga
    29161 gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa cgcgatgaaa
    29221 agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc ctctttcatg
    29281 acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt agccgcccag
    29341 acggaacaaa atccgcaggt ggggcaagtg atgtcaggcg agcagggagc tgatgccccc
    29401 atcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca aatcgcgccg
    29461 ccgccgggca gcatggtgct acgtggtatt aacccgaacg gcagcattga gtttggtatg
    29521 cgcagcgatg aagtggtgac gaaggcgatg ctcaacttcg aatacacgcc atcgccatcg
    29581 ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgagctgat gggcgtgctg
    29641 ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat taatccactg
    29701 tttattaccg acttcaaccg tgtgcggctg gagtttgtcg gccattatca ggacgtgtgc
    29761 gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg actggatctg
    29821 acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc attctttgac
    29881 ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtgcgcc ggatgttggg
    29941 ctgcaacaag catctgccat tgtcgcctcg tggtttggtt cgcgttctgg ctggcgtggg
    30001 cagaacttcc cggtgctcta taaccaattg ccggatcgca atgccattgt ctttgcaacc
    30061 aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc ggtgattgag
    30121 atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg tcgtgacgac
    30181 aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt ccgtggtgaa
    30241 agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga tgcgccgaac
    30301 tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga aaacctatga agaacagtta
    30361 caatccagcg gccttgagcc agcagcgatt aacgtttcgc taaacctgcc gccggatctc
    30421 tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac catgccgccg
    30481 gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca atccttcaac
    30541 ctgagcagca aacaggaggc gaaccgtctg ctgctgcgta ttccggtatt acaaggtttg
    30601 ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac caaccagctg
    30661 cgcttcgact ttgagtatat gaacccgatg ccaggcggtt cggtagataa ctgtattacc
    30721 ttccagccgg tgcagaatca tgtggtgatt ggtgacgatt ccaccatcga cttctcgaag
    30781 tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg cttcccattc
    30841 agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc taacgaagca
    30901 cagatggaaa cgttgctgaa tactgttggt tttatcggcg cacagacggg cttcccggcg
    30961 attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc cgacatcatg
    31021 atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct attggtgcag
    31081 gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc cgttccccgg cattgtgccg
    31141 gacgagagcg atcgcgcggc agaaacccag tcaacgctga cctcttccgg tgcgatggcg
    31201 gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc gctgttggca
    31261 gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg caaacgcgcc
    31321 accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct acgtgttggc
    31381 gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc gctggcaaac
    31441 catccgattc tgctggcggt gctggcgact atcagtgtga tattgctggc atgggtactg
    31501 tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga gtaattgaag
    31561 atgaatgtgt tgcgtagtgg aatcgtgacg atgctgttgt tggctgcctt tagtgttcag
    31621 gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag tcaggaaggg
    31681 cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca aagttacggt
    31741 atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct cgactggacg
    31801 cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct gtggggcaag
    31861 aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg tgatgtctgg
    31921 atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta taccgacatc
    31981 ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc tgggctgggt
    32041 tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg ttttaacccc
    32101 agctacatgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc gtggaccacg
    32161 ctgcgcgaaa ccaatcaaca tttattgctg gaaaccgccc cgaaaggctt ttcgccagac
    32221 tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac attgatcagc
    32281 agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag cgatccgcaa
    32341 aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa aaacggttat
    32401 ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc ggtcggtttt
    32461 tctgccgcca tgctgccttt tttacaaaac cgcgatgcgc aggcagttca gcgccagcgc
    32521 gtggccgata actttcccgg cagcgatacc tattacaact atgtgctgac cctgtttgga
    32581 caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt acctgactgg
    32641 ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt ctggccgtca
    32701 tgccgatggt cgaggcagca ccaaccatca gcagaccctg cggatggaca gcggcaacac
    32761 taatgccgtg cgcgggctgg cgaatattta ccgccagcag tcgccagaaa aagctgaagc
    32821 gtttatcgcc tcgctctctg ccagccagcg gcgcagcatt gatgatatcg aacgcagcct
    32881 gcaaaacgac cgtctggcac agcaggcaga ggtactggaa aaccagggca aatgggcgca
    32941 ggcggcagca cttcagcggc aacgactggc gctgggcccc ggcagcgtat ggattactta
    33001 ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg
    33061 gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg
    33121 caattgatat ccagcaggtg aaagattacg cacccagcca tttcctgctc tacgtacgtt
    33181 attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg
    33241 ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg
    33301 gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca
    33361 agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt
    33421 tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg
    33481 ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg
    33541 aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc
    33601 ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgtccggtg ccggtaatgg
    33661 ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtctactcg ctcgaacgtc
    33721 cggcaaatcc gcagccaata gcctatctgg tgctacaggc ggattccttc cgtatgtata
    33781 agttcgtgat gagcactctc tcaacgttag tgaccattta cttacttttg tcgcttatcc
    33841 tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg
    33901 ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc
    33961 gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat
    34021 tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt
    34081 tgccgaacaa agccttgctg atggagatgc tggagcaggt tgtcgcgcgt aaacaaacca
    34141 ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg
    34201 cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac
    34261 gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg
    34321 aaccgtggca cgcaatcact ttgggtcagc aagtactcac tatcatgagc gagcgcctgc
    34381 cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg
    34441 atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata
    34501 aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagcagcc cagaagcggt
    34561 tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgca atttggttac
    34621 agccacaggt cgagatgacc agcggtaaac tggtcagtgc ggaagtgtta ctgcgtatcc
    34681 agcaaccgga tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg
    34741 ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct
    34801 ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc
    34861 acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa
    34921 cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa
    34981 tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct
    35041 acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctgaaaatcg
    35101 acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc
    35161 tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgag
    35221 actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc
    35281 ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca
    35341 aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca
    35401 attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tat
//
LOCUS       DBBBSB010000028        34996 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.028, whole genome shotgun sequence.
ACCESSION   DBBBSB010000028 DBBBSB010000000
VERSION     DBBBSB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34996
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.028"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..815)
                     /locus_tag="ACIUS0_001396"
     CDS             complement(<1..815)
                     /locus_tag="ACIUS0_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0219208.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACIUS0_001397"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACIUS0_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACIUS0_001398"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACIUS0_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1398..1874)
                     /locus_tag="ACIUS0_001399"
     CDS             complement(1398..1874)
                     /locus_tag="ACIUS0_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGB0219209.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACIUS0_001400"
     CDS             complement(1933..3222)
                     /gene="bioA"
                     /locus_tag="ACIUS0_001400"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGB0219210.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3309..4349
                     /gene="bioB"
                     /locus_tag="ACIUS0_001401"
     CDS             3309..4349
                     /gene="bioB"
                     /locus_tag="ACIUS0_001401"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGB0219211.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4346..5500
                     /gene="bioF"
                     /locus_tag="ACIUS0_001402"
     CDS             4346..5500
                     /gene="bioF"
                     /locus_tag="ACIUS0_001402"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGB0219212.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5487..6242
                     /gene="bioC"
                     /locus_tag="ACIUS0_001403"
     CDS             5487..6242
                     /gene="bioC"
                     /locus_tag="ACIUS0_001403"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGB0219213.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6235..6912
                     /gene="bioD"
                     /locus_tag="ACIUS0_001404"
     CDS             6235..6912
                     /gene="bioD"
                     /locus_tag="ACIUS0_001404"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGB0219214.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7491..9512
                     /gene="uvrB"
                     /locus_tag="ACIUS0_001405"
     CDS             7491..9512
                     /gene="uvrB"
                     /locus_tag="ACIUS0_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGB0219215.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKKFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACIUS0_001406"
     CDS             complement(9704..10612)
                     /gene="yvcK"
                     /locus_tag="ACIUS0_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGB0219216.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10877..11021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11009..11998
                     /gene="moaA"
                     /locus_tag="ACIUS0_001407"
     CDS             11009..11998
                     /gene="moaA"
                     /locus_tag="ACIUS0_001407"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGB0219217.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12020..12532
                     /gene="moaB"
                     /locus_tag="ACIUS0_001408"
     CDS             12020..12532
                     /gene="moaB"
                     /locus_tag="ACIUS0_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGB0219218.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12535..13020
                     /gene="moaC"
                     /locus_tag="ACIUS0_001409"
     CDS             12535..13020
                     /gene="moaC"
                     /locus_tag="ACIUS0_001409"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGB0219219.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13013..13258
                     /gene="moaD"
                     /locus_tag="ACIUS0_001410"
     CDS             13013..13258
                     /gene="moaD"
                     /locus_tag="ACIUS0_001410"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGB0219220.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13260..13712
                     /gene="moaE"
                     /locus_tag="ACIUS0_001411"
     CDS             13260..13712
                     /gene="moaE"
                     /locus_tag="ACIUS0_001411"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGB0219221.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13849..14553
                     /locus_tag="ACIUS0_001412"
     CDS             13849..14553
                     /locus_tag="ACIUS0_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGB0219222.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14758..15471
                     /locus_tag="ACIUS0_001413"
     CDS             14758..15471
                     /locus_tag="ACIUS0_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGB0219223.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15507..16463)
                     /locus_tag="ACIUS0_001414"
     CDS             complement(15507..16463)
                     /locus_tag="ACIUS0_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGB0219224.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACIUS0_001415"
     CDS             complement(16463..17704)
                     /gene="clsB"
                     /locus_tag="ACIUS0_001415"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGB0219225.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17701..18462)
                     /locus_tag="ACIUS0_001416"
     CDS             complement(17701..18462)
                     /locus_tag="ACIUS0_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGB0219226.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18595..19005
                     /locus_tag="ACIUS0_001417"
     CDS             18595..19005
                     /locus_tag="ACIUS0_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGB0219227.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18967..20073)
                     /locus_tag="ACIUS0_001418"
     CDS             complement(18967..20073)
                     /locus_tag="ACIUS0_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0219228.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20084..21217)
                     /locus_tag="ACIUS0_001419"
     CDS             complement(20084..21217)
                     /locus_tag="ACIUS0_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0219229.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21210..22946)
                     /locus_tag="ACIUS0_001420"
     CDS             complement(21210..22946)
                     /locus_tag="ACIUS0_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGB0219230.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACIUS0_001421"
     CDS             complement(22939..23934)
                     /gene="hlyD"
                     /locus_tag="ACIUS0_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGB0219231.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACIUS0_001422"
     CDS             complement(23937..24608)
                     /gene="cecR"
                     /locus_tag="ACIUS0_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGB0219232.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24837..26057
                     /gene="rhlE"
                     /locus_tag="ACIUS0_001423"
     CDS             24837..26057
                     /gene="rhlE"
                     /locus_tag="ACIUS0_001423"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGB0219233.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26051..26149)
                     /locus_tag="ACIUS0_001424"
                     /pseudo
     CDS             complement(26051..26149)
                     /locus_tag="ACIUS0_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACIUS0_001425"
     CDS             complement(26231..26713)
                     /gene="ybiA"
                     /locus_tag="ACIUS0_001425"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGB0219234.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26833..28983
                     /gene="dinG"
                     /locus_tag="ACIUS0_001426"
     CDS             26833..28983
                     /gene="dinG"
                     /locus_tag="ACIUS0_001426"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGB0219235.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29011..29973
                     /gene="ybiB"
                     /locus_tag="ACIUS0_001427"
     CDS             29011..29973
                     /gene="ybiB"
                     /locus_tag="ACIUS0_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGB0219236.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30114..31199
                     /gene="hcxB"
                     /locus_tag="ACIUS0_001428"
     CDS             30114..31199
                     /gene="hcxB"
                     /locus_tag="ACIUS0_001428"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGB0219237.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31266..31325
                     /gene="ybiE"
                     /locus_tag="ACIUS0_001429"
     CDS             31266..31325
                     /gene="ybiE"
                     /locus_tag="ACIUS0_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGB0219238.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACIUS0_001430"
     CDS             complement(31428..31688)
                     /gene="ybiJ"
                     /locus_tag="ACIUS0_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGB0219239.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACIUS0_001431"
     CDS             complement(31953..32219)
                     /gene="ybiI"
                     /locus_tag="ACIUS0_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGB0219240.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACIUS0_001432"
     CDS             complement(32293..32970)
                     /gene="ybiX"
                     /locus_tag="ACIUS0_001432"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGB0219241.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33012..34996)
                     /locus_tag="ACIUS0_001433"
                     /pseudo
     CDS             complement(33012..34996)
                     /locus_tag="ACIUS0_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8972 c         9453 g         8276 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg
     1321 ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc
     1381 gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg
     1441 aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct
     1501 acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg
     1561 ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct
     1621 accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact
     1681 acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa
     1741 cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg
     1801 taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta
     1861 ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1921 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc
     1981 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     2041 gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt
     2101 caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat
     2161 ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg
     2221 ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag
     2281 cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt
     2341 ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc
     2401 tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc
     2461 ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca
     2521 cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg
     2581 gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc
     2641 aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc
     2701 aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca
     2761 caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg
     2821 gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc
     2881 tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag
     2941 tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat
     3001 ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc
     3061 cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca
     3121 accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg
     3181 ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa
     3241 attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga
     3301 gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa
     3361 aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc
     3421 gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca
     3481 aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag
     3541 ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta
     3601 tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc
     3661 agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc
     3721 aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc
     3781 cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg
     3841 aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa
     3901 cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa
     3961 gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg
     4021 ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt
     4081 acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta
     4141 tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag
     4201 aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc
     4261 tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg
     4321 acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga
     4381 tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg
     4441 ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt
     4501 aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg
     4561 tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga
     4621 actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa
     4681 tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag
     4741 ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca
     4801 taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt
     4861 ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca
     4921 ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat
     4981 cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt
     5041 gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc
     5101 ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca
     5161 ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg
     5221 cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac
     5281 gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt
     5341 acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac
     5401 cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga
     5461 tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg
     5521 catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg
     5581 acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg
     5641 gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc
     5701 tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag
     5761 gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg
     5821 cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc
     5881 ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg
     5941 cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg
     6001 aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg
     6061 atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag
     6121 ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc
     6181 aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt
     6241 aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca
     6301 cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct
     6361 ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac
     6421 agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg
     6481 ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga
     6541 ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt
     6601 acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg
     6661 atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca
     6721 atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga
     6781 aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga
     6841 gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt
     6901 gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga
     6961 ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat
     7021 gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact
     7081 attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg
     7141 cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact
     7201 ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca
     7261 ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt
     7321 tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag
     7381 aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac
     7441 gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac
     7501 cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc
     7561 tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg
     7621 ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg
     7681 cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaaaagttc ttcccggaaa
     7741 acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga
     7801 gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt
     7861 tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg
     7921 cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg
     7981 gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta
     8041 atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc
     8101 cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat
     8161 tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct
     8221 acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca
     8281 aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc
     8341 agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt
     8401 cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga
     8461 cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca
     8521 ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt
     8581 acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag
     8641 cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat
     8701 ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg
     8761 aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg
     8821 caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg
     8881 aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg
     8941 agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca
     9001 acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg
     9061 acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac
     9121 gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag
     9181 cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca
     9241 ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg
     9301 ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc
     9361 cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga
     9421 tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc
     9481 agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa
     9541 cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa
     9601 tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca
     9661 ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta
     9721 acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg
     9781 cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc
     9841 cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc
     9901 tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa
     9961 ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca
    10021 tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt
    10081 gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag
    10141 ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca
    10201 gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa
    10261 ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt
    10321 ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg
    10381 aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa
    10441 tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga
    10501 tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc
    10561 ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc
    10621 ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg
    10681 ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct
    10741 ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca
    10801 cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt
    10861 acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata
    10921 cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac
    10981 ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg
    11041 taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg
    11101 cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga
    11161 aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg
    11221 aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga
    11281 cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa
    11341 ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca
    11401 gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc
    11461 atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca
    11521 tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat
    11581 cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca
    11641 ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga
    11701 cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc
    11761 gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact
    11821 ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac
    11881 ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca
    11941 tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa
    12001 cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc
    12061 tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg
    12121 cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg
    12181 ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt
    12241 gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt
    12301 gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat
    12361 tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt
    12421 cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca
    12481 gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg
    12541 caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg
    12601 gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg
    12661 atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt
    12721 attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc
    12781 aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta
    12841 tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg
    12901 gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta
    12961 cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa
    13021 agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc
    13081 ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc
    13141 gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga
    13201 ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga
    13261 tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt
    13321 ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc
    13381 ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa
    13441 aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg
    13501 tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca
    13561 gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc
    13621 gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg
    13681 agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct
    13741 gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat
    13801 cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc
    13861 ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc
    13921 tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat
    13981 tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc
    14041 gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta
    14101 acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc
    14161 attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc
    14221 gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg
    14281 tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa
    14341 gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat
    14401 gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg
    14461 cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg
    14521 atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc
    14581 atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat
    14641 gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg
    14701 tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg
    14761 gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac
    14821 ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct
    14881 aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg
    14941 ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc
    15001 gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt
    15061 cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg
    15121 ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg
    15181 ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt
    15241 tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat
    15301 aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc
    15361 gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc
    15421 gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg
    15481 cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc
    15541 ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa
    15601 tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca
    15661 gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg
    15721 acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga
    15781 ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc
    15841 tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg
    15901 cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg
    15961 ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct
    16021 gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc
    16081 gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc
    16141 ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac
    16201 accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca
    16261 tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg
    16321 cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc
    16381 accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc
    16441 cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat
    16501 cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg
    16561 ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt
    16621 tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg
    16681 cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga
    16741 tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg
    16801 tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa
    16861 aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa
    16921 tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt
    16981 tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt
    17041 cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt
    17101 cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt
    17161 tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa
    17221 actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct
    17281 ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac
    17341 gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa
    17401 aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga
    17461 caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc
    17521 cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc
    17581 agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg
    17641 gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt
    17701 tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag
    17761 cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag
    17821 tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc
    17881 atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc
    17941 acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc
    18001 aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc
    18061 ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc
    18121 gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc
    18181 gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc
    18241 ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc
    18301 gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc
    18361 gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat
    18421 ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc
    18481 tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt
    18541 ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag
    18601 tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta
    18661 ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg
    18721 ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc
    18781 gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt
    18841 ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg
    18901 gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat
    18961 aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt
    19021 tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata
    19081 aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag
    19141 ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat
    19201 aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag
    19261 accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga
    19321 tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa
    19381 ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg
    19441 cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt
    19501 gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt
    19561 gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg
    19621 ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc
    19681 aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa
    19741 ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg ccttttgtgt
    19801 gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct
    19861 ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat
    19921 ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat
    19981 tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc
    20041 tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt
    20101 tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt
    20161 ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga
    20221 aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct
    20281 gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag
    20341 cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga
    20401 gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga
    20461 gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag
    20521 ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca
    20581 tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg
    20641 tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa
    20701 accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc
    20761 gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat
    20821 tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct
    20881 gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg
    20941 cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg
    21001 tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct
    21061 tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta
    21121 ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg
    21181 cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg
    21241 tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac
    21301 tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg
    21361 atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct
    21421 ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg
    21481 gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc
    21541 agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg
    21601 ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac
    21661 acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt
    21721 tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc
    21781 agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc
    21841 gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag
    21901 ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc
    21961 accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag
    22021 gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg
    22081 atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg
    22141 aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag
    22201 gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg
    22261 gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc
    22321 agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac
    22381 atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca
    22441 acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc
    22501 aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc
    22561 ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg
    22621 ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca
    22681 aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca
    22741 aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc
    22801 agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg
    22861 gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg
    22921 ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg
    22981 cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag
    23041 gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac
    23101 gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt
    23161 gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg
    23221 acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac
    23281 cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag
    23341 ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc
    23401 ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc
    23461 ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct
    23521 tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc
    23581 ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag
    23641 atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata
    23701 cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac
    23761 cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac
    23821 gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc
    23881 aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac
    23941 aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg
    24001 atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct
    24061 ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg
    24121 gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc
    24181 ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg
    24241 gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat catgttcctg
    24301 caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag
    24361 cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg
    24421 gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc
    24481 tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc
    24541 gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga
    24601 ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg
    24661 gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct
    24721 gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct
    24781 tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt
    24841 ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc
    24901 gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga
    24961 tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac
    25021 acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta
    25081 ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc
    25141 tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt
    25201 tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc
    25261 aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc
    25321 tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc
    25381 agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt
    25441 tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc
    25501 agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta
    25561 aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg
    25621 ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag
    25681 gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga
    25741 ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac
    25801 tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta
    25861 ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa
    25921 aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa
    25981 tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca
    26041 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg
    26101 aatttgcatg cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca
    26161 agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc
    26221 caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca
    26281 gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct
    26341 caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga
    26401 atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa
    26461 gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga
    26521 tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg
    26581 gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt
    26641 catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg
    26701 ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca
    26761 gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt
    26821 tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag
    26881 gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg
    26941 gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc
    27001 ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa
    27061 aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat
    27121 ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg
    27181 cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat
    27241 ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt
    27301 gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat
    27361 atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac
    27421 cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa
    27481 gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta
    27541 ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg
    27601 gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg
    27661 gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca
    27721 ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc
    27781 tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt
    27841 gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc
    27901 gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc
    27961 cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt
    28021 gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg
    28081 accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg
    28141 ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt
    28201 gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt
    28261 ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa
    28321 cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa
    28381 cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt
    28441 atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat
    28501 ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac
    28561 cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa
    28621 gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg
    28681 cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat
    28741 ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga
    28801 ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca
    28861 aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa
    28921 gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt
    28981 taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc
    29041 gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct
    29101 catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt
    29161 atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc
    29221 atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc
    29281 gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt
    29341 cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc
    29401 gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa
    29461 catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg
    29521 atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca
    29581 tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc
    29641 gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc
    29701 gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg
    29761 cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat
    29821 ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa
    29881 tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc
    29941 gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt
    30001 catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc
    30061 ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa
    30121 gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga
    30181 tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg
    30241 cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg
    30301 ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc
    30361 tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg
    30421 agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc
    30481 gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta
    30541 gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca
    30601 atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca
    30661 ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc
    30721 caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt
    30781 cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt
    30841 gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa
    30901 ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg
    30961 gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc
    31021 atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac
    31081 ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc
    31141 ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa
    31201 aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag
    31261 agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg
    31321 cctgatccgt ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca
    31381 ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt
    31441 accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca
    31501 ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggt ggatgcgcca
    31561 tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc
    31621 gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata
    31681 gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt
    31741 gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa
    31801 ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg
    31861 ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa
    31921 attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct
    31981 ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata
    32041 cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct
    32101 tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct
    32161 tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata
    32221 tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg
    32281 gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag
    32341 cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag
    32401 ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga
    32461 tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa
    32521 caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag
    32581 ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg
    32641 catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc
    32701 attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa
    32761 gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc
    32821 gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac
    32881 gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg
    32941 cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc
    33001 ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg
    33061 tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa
    33121 acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc
    33181 cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg
    33241 ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat
    33301 aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct
    33361 ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga
    33421 gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag
    33481 taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg
    33541 gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct
    33601 ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg
    33661 aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt
    33721 tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg
    33781 tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt
    33841 ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc
    33901 ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag
    33961 gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg
    34021 ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc
    34081 tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag
    34141 aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc
    34201 gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc
    34261 ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg
    34321 cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc
    34381 gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga
    34441 ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg
    34501 ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct
    34561 gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg
    34621 cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca
    34681 atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag
    34741 gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt
    34801 gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg
    34861 aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag
    34921 gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca
    34981 aaaaacgcac ccacgc
//
LOCUS       DBBBSB010000029        34903 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.029, whole genome shotgun sequence.
ACCESSION   DBBBSB010000029 DBBBSB010000000
VERSION     DBBBSB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.029"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACIUS0_001434"
     CDS             complement(587..730)
                     /gene="ypaB"
                     /locus_tag="ACIUS0_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGB0219242.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="ACIUS0_001435"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="ACIUS0_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGB0219243.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="ACIUS0_001436"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="ACIUS0_001436"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGB0219244.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="ACIUS0_001437"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="ACIUS0_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGB0219245.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACIUS0_001438"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="ACIUS0_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGB0219246.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5545..5649)
                     /locus_tag="ACIUS0_001439"
                     /pseudo
     CDS             complement(5545..5649)
                     /locus_tag="ACIUS0_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5644..5886
                     /locus_tag="ACIUS0_001440"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="ACIUS0_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACIUS0_001441"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="ACIUS0_001441"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGB0219247.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACIUS0_001442"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="ACIUS0_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGB0219248.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="ACIUS0_001443"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="ACIUS0_001443"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGB0219249.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="ACIUS0_001444"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="ACIUS0_001444"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGB0219250.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="ACIUS0_001445"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="ACIUS0_001445"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGB0219251.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="ACIUS0_001446"
     CDS             12897..13847
                     /locus_tag="ACIUS0_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGB0219252.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14349)
                     /locus_tag="ACIUS0_001447"
                     /pseudo
     CDS             complement(13888..14349)
                     /locus_tag="ACIUS0_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14413..15615)
                     /locus_tag="ACIUS0_001448"
     CDS             complement(14413..15615)
                     /locus_tag="ACIUS0_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGB0219253.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="ACIUS0_001449"
     CDS             complement(15702..16244)
                     /locus_tag="ACIUS0_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGB0219254.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="ACIUS0_001450"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="ACIUS0_001450"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGB0219255.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACIUS0_001451"
     CDS             complement(16987..17589)
                     /gene="ais"
                     /locus_tag="ACIUS0_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGB0219256.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="ACIUS0_001452"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="ACIUS0_001452"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGB0219257.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="ACIUS0_001453"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="ACIUS0_001453"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGB0219258.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="ACIUS0_001454"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="ACIUS0_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /gene="arnD"
                     /locus_tag="ACIUS0_001455"
     CDS             21986..22876
                     /gene="arnD"
                     /locus_tag="ACIUS0_001455"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGB0219259.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="ACIUS0_001456"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="ACIUS0_001456"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGB0219260.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="ACIUS0_001457"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="ACIUS0_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGB0219261.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="ACIUS0_001458"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="ACIUS0_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGB0219262.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACIUS0_001459"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="ACIUS0_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGB0219263.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACIUS0_001460"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="ACIUS0_001460"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGB0219264.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACIUS0_001461"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="ACIUS0_001461"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGB0219265.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACIUS0_001462"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="ACIUS0_001462"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGB0219266.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACIUS0_001463"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="ACIUS0_001463"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGB0219267.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACIUS0_001464"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="ACIUS0_001464"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGB0219268.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACIUS0_001465"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="ACIUS0_001465"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGB0219269.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACIUS0_001466"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="ACIUS0_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGB0219270.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="ACIUS0_001467"
     CDS             complement(33051..33512)
                     /locus_tag="ACIUS0_001467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0219271.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="ACIUS0_001468"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="ACIUS0_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGB0219272.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="ACIUS0_001469"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="ACIUS0_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8539 a         9338 c         8647 g         8379 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcacca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttataga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgttgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggcagcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DBBBSB010000030        34851 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.030, whole genome shotgun sequence.
ACCESSION   DBBBSB010000030 DBBBSB010000000
VERSION     DBBBSB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.030"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(18..93)
                     /locus_tag="ACIUS0_001470"
     tRNA            complement(18..93)
                     /locus_tag="ACIUS0_001470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACIUS0_001471"
     tRNA            complement(152..228)
                     /locus_tag="ACIUS0_001471"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="ACIUS0_001472"
     CDS             complement(331..1716)
                     /locus_tag="ACIUS0_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGB0219273.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACIUS0_001473"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACIUS0_001473"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGB0219274.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACIUS0_001474"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACIUS0_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGB0219275.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACIUS0_001475"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACIUS0_001475"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGB0219276.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACIUS0_001476"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACIUS0_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGB0219277.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACIUS0_001477"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACIUS0_001477"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGB0219278.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACIUS0_001478"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACIUS0_001478"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGB0219279.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACIUS0_001479"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACIUS0_001479"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGB0219280.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACIUS0_001480"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACIUS0_001480"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGB0219281.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACIUS0_001481"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACIUS0_001481"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGB0219282.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACIUS0_001482"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACIUS0_001482"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGB0219283.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACIUS0_001483"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACIUS0_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGB0219284.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACIUS0_001484"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACIUS0_001484"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGB0219285.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACIUS0_001485"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACIUS0_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGB0219286.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACIUS0_001486"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACIUS0_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGB0219287.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACIUS0_001487"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACIUS0_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGB0219288.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACIUS0_001488"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACIUS0_001488"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGB0219289.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACIUS0_001489"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACIUS0_001489"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGB0219290.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACIUS0_001490"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACIUS0_001490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGB0219291.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="ACIUS0_001491"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="ACIUS0_001491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGB0219292.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="ACIUS0_001492"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="ACIUS0_001492"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGB0219293.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="ACIUS0_001493"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="ACIUS0_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGB0219294.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="ACIUS0_001494"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="ACIUS0_001494"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGB0219295.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="ACIUS0_001495"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="ACIUS0_001495"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGB0219296.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="ACIUS0_001496"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="ACIUS0_001496"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGB0219297.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="ACIUS0_001497"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="ACIUS0_001497"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGB0219298.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /gene="ilvG"
                     /locus_tag="ACIUS0_001498"
     CDS             complement(29521..31167)
                     /gene="ilvG"
                     /locus_tag="ACIUS0_001498"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGB0219299.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /gene="ilvX"
                     /locus_tag="ACIUS0_001499"
     CDS             complement(31170..31220)
                     /gene="ilvX"
                     /locus_tag="ACIUS0_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGB0219300.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="ACIUS0_001500"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="ACIUS0_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGB0219301.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="ACIUS0_001501"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="ACIUS0_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="ACIUS0_001502"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="ACIUS0_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGB0219302.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="ACIUS0_001503"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="ACIUS0_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGB0219303.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34652..34727)
                     /locus_tag="ACIUS0_001504"
     tRNA            complement(34652..34727)
                     /locus_tag="ACIUS0_001504"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
     gene            complement(34736..34812)
                     /locus_tag="ACIUS0_001505"
     tRNA            complement(34736..34812)
                     /locus_tag="ACIUS0_001505"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34776..34778),aa:Asp,seq:gtc)
BASE COUNT         8501 a         9538 c         8801 g         8011 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ctgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    34741 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    34801 aactaccgct ccaccgaatt cttttacaac caccggtttt acgaccggct t
//
LOCUS       DBBBSB010000031        34788 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.031, whole genome shotgun sequence.
ACCESSION   DBBBSB010000031 DBBBSB010000000
VERSION     DBBBSB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.031"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..2098
                     /locus_tag="ACIUS0_001506"
     CDS             <1..2098
                     /locus_tag="ACIUS0_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGB0219304.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACIUS0_001507"
     CDS             2095..3183
                     /locus_tag="ACIUS0_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HGB0219305.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACIUS0_001508"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACIUS0_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGB0219306.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACIUS0_001509"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACIUS0_001509"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGB0219307.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACIUS0_001510"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACIUS0_001510"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGB0219308.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACIUS0_001511"
     CDS             8051..8602
                     /locus_tag="ACIUS0_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGB0219309.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACIUS0_001512"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACIUS0_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGB0219310.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACIUS0_001513"
     CDS             complement(10447..11412)
                     /gene="alx"
                     /locus_tag="ACIUS0_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGB0219311.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11683..12681)
                     /locus_tag="ACIUS0_001514"
     CDS             complement(11683..12681)
                     /locus_tag="ACIUS0_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGB0219312.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12760..13452)
                     /locus_tag="ACIUS0_001515"
     CDS             complement(12760..13452)
                     /locus_tag="ACIUS0_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGB0219313.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13529..14032)
                     /locus_tag="ACIUS0_001516"
     CDS             complement(13529..14032)
                     /locus_tag="ACIUS0_001516"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGB0219314.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14117..15253
                     /gene="rlmG"
                     /locus_tag="ACIUS0_001517"
     CDS             14117..15253
                     /gene="rlmG"
                     /locus_tag="ACIUS0_001517"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGB0219315.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15538..15852
                     /gene="higB"
                     /locus_tag="ACIUS0_001518"
     CDS             15538..15852
                     /gene="higB"
                     /locus_tag="ACIUS0_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGB0219316.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15849..16265
                     /gene="higA"
                     /locus_tag="ACIUS0_001519"
     CDS             15849..16265
                     /gene="higA"
                     /locus_tag="ACIUS0_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGB0219317.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACIUS0_001520"
     CDS             complement(16310..18328)
                     /gene="fadH"
                     /locus_tag="ACIUS0_001520"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGB0219318.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACIUS0_001521"
     CDS             complement(18554..20905)
                     /gene="ygjK"
                     /locus_tag="ACIUS0_001521"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGB0219319.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACIUS0_001522"
                     /pseudo
     CDS             complement(20922..21993)
                     /gene="ygjJ"
                     /locus_tag="ACIUS0_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22127..23560)
                     /locus_tag="ACIUS0_001523"
     CDS             complement(22127..23560)
                     /locus_tag="ACIUS0_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGB0219320.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23623..24072)
                     /locus_tag="ACIUS0_001524"
     CDS             complement(23623..24072)
                     /locus_tag="ACIUS0_001524"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGB0219321.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACIUS0_001525"
     CDS             complement(24069..27155)
                     /gene="ebgA"
                     /locus_tag="ACIUS0_001525"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGB0219322.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACIUS0_001526"
     CDS             complement(27338..28321)
                     /gene="ebgR"
                     /locus_tag="ACIUS0_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGB0219323.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28539..28870
                     /locus_tag="ACIUS0_001527"
                     /pseudo
     CDS             28539..28870
                     /locus_tag="ACIUS0_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACIUS0_001528"
     CDS             complement(28912..30291)
                     /gene="ygjG"
                     /locus_tag="ACIUS0_001528"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGB0219324.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30709..32229
                     /gene="aer"
                     /locus_tag="ACIUS0_001529"
                     /pseudo
     CDS             30709..32229
                     /gene="aer"
                     /locus_tag="ACIUS0_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACIUS0_001530"
                     /gene_synonym="yqjI"
     CDS             complement(32383..33006)
                     /gene="nfeR"
                     /locus_tag="ACIUS0_001530"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGB0219325.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33283..34028
                     /gene="nfeF"
                     /locus_tag="ACIUS0_001531"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             33283..34028
                     /gene="nfeF"
                     /locus_tag="ACIUS0_001531"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(34082..34157)
                     /locus_tag="ACIUS0_001532"
     tRNA            complement(34082..34157)
                     /locus_tag="ACIUS0_001532"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34122..34124),aa:Ile,seq:cat)
     gene            34282..34788
                     /gene="mug"
                     /locus_tag="ACIUS0_001533"
                     /pseudo
     CDS             34282..34788
                     /gene="mug"
                     /locus_tag="ACIUS0_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8482 a         8945 c         9143 g         8218 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtgat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg cacccccagg
    10201 ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc ggctaccggg
    10261 tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca gggaacgctt
    10321 cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta taagccgatt
    10381 acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac
    10441 agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa
    10501 cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa
    10561 gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc catatttgag
    10621 catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat
    10681 tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg
    10741 aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag
    10801 cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc
    10861 ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc
    10921 atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata
    10981 caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga tggtacgcag
    11041 aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg ccggaacgga
    11101 gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat
    11161 cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac cttcggtttg
    11221 caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga
    11281 ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag
    11341 gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc
    11401 gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc
    11461 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    11521 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cactcatcgt
    11581 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    11641 attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg gcaagtatag
    11701 ttttacgcta tctgccggaa aaatcacccc agtttggcgg cggatctcgg tcagcagttt
    11761 ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    11821 agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    11881 catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt tttcaatcac
    11941 cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga cctttgagtg
    12001 ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg cgtcaacgcc
    12061 gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc
    12121 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    12181 ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    12241 gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca aatgaaagtt
    12301 cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc gcgcacaggc
    12361 aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa tcacatgaat
    12421 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc tggcaatata
    12481 caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca gatgctcgac
    12541 agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata cggcggttaa
    12601 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    12661 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    12721 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg ccggacagcc
    12781 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggcgatggag
    12841 gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa
    12901 accgtgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc cgtactgttt
    12961 tgccagccag atggtgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    13021 cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    13081 aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc
    13141 atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt ttccggcgtg
    13201 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc
    13261 cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg tcagctgctt
    13321 gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    13381 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa
    13441 tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    13501 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    13561 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    13621 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    13681 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctctgctt
    13741 ttgctttcag cttgctgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    13801 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa
    13861 actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg tagtcgtgtg
    13921 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca
    13981 cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc
    14041 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    14101 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    14161 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag atgaatattt
    14221 gctgcaacag ttggacgaca cagaaatcct cggcccggtg ttgatcctga atgatgcctt
    14281 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    14341 cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt cgagcgtgaa
    14401 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    14461 gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    14521 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    14581 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    14641 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    14701 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    14761 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    14821 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    14881 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    14941 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15001 gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac atgcgctgac
    15061 cgataacgtc gcctgggaga tgttccacta cgcccgccgc tgcctgaaaa tcaacggcga
    15121 gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga aaattttcgg
    15181 caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg tgaagctggg
    15241 acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct
    15301 acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    15361 ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca
    15421 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    15481 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    15541 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    15601 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    15661 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    15721 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    15781 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    15841 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    15901 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    15961 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa
    16021 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    16081 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    16141 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    16201 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttgtttattg
    16261 attaacgcga cgtgccgaat gcgttgcatc cggcgcttca aataacgatt taaatctcca
    16321 gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata gccacatcgc
    16381 agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc agcgcgcggt
    16441 ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc gtttcgccgt
    16501 tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct gggatcattt
    16561 tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg cctaacccct
    16621 gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta cgcgggatct
    16681 gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc ccccactcat
    16741 tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa tacatcgccg
    16801 tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc ggcgctttgt
    16861 cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg ataggcggaa
    16921 cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac tgatccgccg
    16981 tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg cggtagtagc
    17041 gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta aactgcccgc
    17101 cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc gcgttaatgg
    17161 caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc tgcacggcgg
    17221 gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac gaggtgactt
    17281 tgccaacgaa gatctgatcg aggcagccct ggttgcagcc aatacaagtg ttgatctcat
    17341 cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac ggtcgcgcca
    17401 tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc ggatcgttaa
    17461 tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc gtgacccagc
    17521 taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg tgccagccta
    17581 ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt tctaccgttt
    17641 cggcaaaagt gccgccgcct tcgactaggt cgagcatcga caaccggtaa ataataatga
    17701 agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc tacggcaaat cgcatccggt
    17761 tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc agaaattcgt
    17821 tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc tcccgcgcca
    17881 gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg gtgagttcgt
    17941 gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga tgcggttggt
    18001 agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc tgatgtacgg
    18061 cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt gcgccacctt
    18121 ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc gccacgccgt
    18181 gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg tattcctcca
    18241 gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa cctaaatcca
    18301 gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat aattgttatg
    18361 tagtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg agtgcggggt
    18421 ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac gcttgccgtg
    18481 ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc
    18541 cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac aaatgagcgg
    18601 cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg taattctcct
    18661 gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc gccagcttca
    18721 gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag aactgatcca
    18781 cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt aacgccgccg
    18841 ttcccagcgg aacaaatgtg ttgaactctt taggatcgag catcaccttc accaccgcgt
    18901 cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc ggccctttac
    18961 cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct tcaatacgta
    19021 cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag tccgcgagct
    19081 gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg gttgccatct
    19141 ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac tcctgcaata
    19201 gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc accgtccagt
    19261 cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg ataaaaccaa
    19321 agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc ggaatttcca
    19381 gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca gactgcgtct
    19441 cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg ttgtgggctt
    19501 tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg ttacgtaacc
    19561 accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct ttatcctggg
    19621 tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta ttacgttcgt
    19681 tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc aggtcgggga
    19741 caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag gagaagaccg
    19801 cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac gcctgcttcc
    19861 aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc gacggcgtga
    19921 cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc gtttcgatcg
    19981 ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac cctttcttca
    20041 ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc gccagaatat
    20101 cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg gaataggtgg
    20161 tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta ccgttgattt
    20221 cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag gtcagcagat
    20281 cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg gcgctgattt
    20341 tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt ttcccttctt
    20401 tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt ttattgctga
    20461 tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc atttcgacct
    20521 gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat gcctccagcg
    20581 tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg ctggccataa
    20641 agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg gtgttcgggc
    20701 cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta aaacgctggt
    20761 gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta atcacgtttt
    20821 tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg caggttactg
    20881 gcgttaaaat agttttgatt ttcatgagat tacagtcctt attaccagta gaaatattgc
    20941 atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa gcggctatca
    21001 aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg catgaaagag
    21061 gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc ggtggagaga
    21121 taaacgcctt ttaacgcttc atacatggcg ctcaccatca tcccgctttc acttcccggt
    21181 acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa gttaggggta
    21241 aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc attcatccag
    21301 gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg gataggtgta
    21361 tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt cgtcatgatg
    21421 tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct gtaccttcag
    21481 cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg tgaagtcgcc
    21541 ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga tttcggtgcc
    21601 atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac caccccagct
    21661 atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc cttcgccgta
    21721 aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat aggtgccgtc
    21781 gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc ctaattcgcc
    21841 gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg tgtcgtcatg
    21901 ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg gcagagcaag
    21961 taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc taaagaaaaa
    22021 gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt ttccctctcc
    22081 tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa tcaacattgc
    22141 tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata tatttgctgt
    22201 atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa aaaatgatgg
    22261 tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca aatatcgcaa
    22321 tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa tcgcgtggca
    22381 ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac ggcggaagca
    22441 tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg gtattggaac
    22501 cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat gctgcgcgcg
    22561 ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct tccgggattt
    22621 cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg aacattgcgg
    22681 taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca aaatacgccg
    22741 ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg ctgctgacga
    22801 agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc ccggcgagga
    22861 tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac accgcgacgg
    22921 actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac gcccagttga
    22981 agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgccg ccaaccagtg
    23041 ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt gaagtgacgg
    23101 aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag aaggcgaaca
    23161 gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag ccgaggaagg
    23221 catacgaagc ataagcaata acgcgcggca acagcgaggt gaaaaagaac agatttacga
    23281 accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt tttacccacg
    23341 cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg atcagacaga
    23401 agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc agtccaagct
    23461 caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc agcgagagca
    23521 agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc cgaaaatgtg
    23581 tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt tatggaaata
    23641 accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc agataaaccg
    23701 atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta cggtttctcc
    23761 gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta cctgtaatgt
    23821 ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca cttcaaaata
    23881 gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac ggtaggtcaa
    23941 tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt caatcgcttc
    24001 aacgcagcgt tgccacttct tgccagaggc gtaaatctgg cggaactgtt ctaagttatc
    24061 gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc tgcgccgaac
    24121 tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag caacgtaaag
    24181 ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct gccccaggag
    24241 ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc actgcgctgc
    24301 agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca ggcgctgaag
    24361 ttaattgggc gctgcggaac caccagcaga ccgttgccgt ggcggttagt cagtgccgtc
    24421 cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc gaacatggca
    24481 tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc gtagttttcg
    24541 cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc catggtgaaa
    24601 ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag cgccacgtta
    24661 acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa gtcaaacacc
    24721 ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg ttctacggca
    24781 aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa cccttcgtac
    24841 tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc gcgggtcagc
    24901 agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga gaaggtgatc
    24961 gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt cagcggacgc
    25021 gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg gaactgataa
    25081 gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt caccgtaatg
    25141 ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg ggcttcgctg
    25201 ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc ttcggcgcgc
    25261 acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt ttcgactttc
    25321 aactcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt gtactctttc
    25381 aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg
    25441 ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg gtcgtcctgt
    25501 gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca atcgtgctta
    25561 tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat
    25621 tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg
    25681 cgggtgtaca tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatga
    25741 accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat gttacagcca
    25801 tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt ctgtgcgtgg
    25861 atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg
    25921 ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac
    25981 aggccgtaga tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc
    26041 acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca
    26101 acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa
    26161 cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt
    26221 ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga
    26281 taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg
    26341 gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca
    26401 aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc cagcaccact
    26461 tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac agtgaaatcg
    26521 ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc cgccgaccac
    26581 cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg cacacacaac
    26641 aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc
    26701 ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca
    26761 aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag
    26821 gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg
    26881 tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc
    26941 ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg atggtcaaaa
    27001 aagtggaaat tccactgact gcttaagggc agaaacaggc tgctggtttc gcgggcaaag
    27061 gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc aagtcggttt
    27121 tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg
    27181 gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa atttttacta
    27241 aaaagcagca ggtaaatgca tttactttaa ctggatcacg aaaataaacg gcagcactgt
    27301 ccggcaaaaa tgattgccga acagtgtgta aggggattta acgggtcgtg ccgcgcagtt
    27361 ttaatttgct gggaacgaag actagcagcg gcagcgcgcg accatcgcgg gctttttcat
    27421 acaccaggtt aacgccctga cttcccatca tctcggaatg gatgcgcacg gtggagagcg
    27481 gcggaaaggt aaatcgcgcg gtggggatat cgttaacgct gataagcgaa atatcttgtg
    27541 ggatgttcag gccacgttca tgaattgccc gcagtacgcc gatagcaatg gaatcggaag
    27601 caacaaacag tgccttcgga tagtcttccc gcgccagcat ttgttttgcc agttcataac
    27661 ccgacgaact ggaaaaaccg ccgcgccaga tatcctcttc gcgtaccact tgtttcagtc
    27721 ggccatattc cgcaaaggcg acctcacgaa tatccgcctt gccaggctca tcttcaccgc
    27781 caataaaacc aatacgatta acgccctggt tgatatagaa gtcgatgatt tctttactga
    27841 tgcgtgccag atcgatatcc accgcatcgt aaccgctgcc gggttcgtga aagtcgataa
    27901 aacagatatt gtcggtcaac gcgctggcag cggcgcgcag ggcgggcgtg ggtttgccga
    27961 caattaaaat accggtgacg tttttaatgt ctggtaatcc gttgtgttca taacagttgg
    28021 tcagttcgat cgccagcttt tcgcactgag tttcaatgcc gtggcggatc gccagatagt
    28081 aaggatcgtt gatctccagc tcctgctggt agctgtagat agccagaata tggtgttggt
    28141 tgactgcgcc tgtctgtagt ttacgggcgc tactggtctt gtactccagc ttttcggcga
    28201 tctcgagaat gcgatgtttc gtctcttctt tcacattcaa tgtcggatcg tcatttaaga
    28261 ccctggatac tgtcgccagg gatacgccag cttcgattgc gatgtctttt aatgttgcca
    28321 tgtttacctt ttttactttc cctgatgtgc gcccccattg tagaggaagt gcttatcggg
    28381 tcacctgttt tcagtaaata aagcgcgttt ctttgacgct gtaacggtaa cacagcggct
    28441 taggaatgtg atcacgcctg cattctccat ttttagccta caggattgag aaaaactttt
    28501 ctatagttta gtaaaatttt actcgaaggg aggaagcaat ggaaaccgtg gcttacgctg
    28561 attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc catgaaaacg
    28621 ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa ccgtgaccag
    28681 cctggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg tcttatgcaa
    28741 tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt gtgcggaaac
    28801 cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg cgggcgtacg
    28861 cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat attacgcttc
    28921 ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca ccagttcaca
    28981 ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt tattgagcgt
    29041 tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc cgatttcgtt
    29101 atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta ccagatcggg
    29161 atattcccgc gccagttgac ggaaaccgtc cagcaacata tcgccttttt gctcagcctg
    29221 agccggtaag ttctgctcca gcaacacatt gatggtcgcc agcgccgccg cacaggccag
    29281 cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg tcgaacagaa ctgaaaacac
    29341 ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg ccgccgaggg cttttgccag
    29401 acacagaatg tccggctgaa cgttctcatg ttcgcaggca aacatcttgc ccgtgcgccc
    29461 catgcccgtt tgtacttcat cgaggatcat cagtgcgccg aactcatcgc acagcttacg
    29521 cacggcggtg agatagcccg gcggcggcag aattacgcca ccttcgccct gaatcggttc
    29581 gaggatcacc gcagccacat catcaccggt ttttttgcac tcattaagag ccgtgcgcat
    29641 ggcttcgata ttgccaaacg gcacatgacg gaaccctggc agcagtggca taaacggttt
    29701 gcggaaggtc gatttcgccg tggccgacag cgcgccaagt gatttaccgt ggaacacgcc
    29761 gctggtggca ataaaagtaa atttgccgcg cggcgactga taagccttcg ccagcttcag
    29821 cgccgcttca acggactcgg tgccgctatt acagaagaag ctgtatttca gtttaccggg
    29881 cgttagcgca gcaagggttt tcgccagcat agcccgtaac ggatcgagta gctcctggct
    29941 gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa accacaactg gattacggtg
    30001 ccccacgttg aaaattccaa aacctcccag gcagtcgata aactcctgtc cctgggtgtc
    30061 gacaagcgta tttaaacctc ccgcttgcca ctctacggct ccgtaatccc cgccggcggt
    30121 aacagatttg cgatactcta aaaaccccgg attgacatgc tctttgaagt attcaatcac
    30181 ctctcggtta agtgctttca tctcctcatg atccagcgtt cgcttctcaa tgagattcag
    30241 ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt aacctgttca aaatatgctc
    30301 cggaggctcg cgtatcacat gataccgatt taagtattgc agggattgcg ccactccggc
    30361 tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc atttgtgcaa tatttaatgc
    30421 tatccgttaa catttgttgt tattttgcac ggtttttgtc gggtatggat ttgatgattg
    30481 ctgcgcaaat gcacgttttc agggcaataa ttgcctgatt tttgggcaag ataattaacg
    30541 ttttcgtata gtaacagagt gaaagtattt taaatcaaag aattagattt gtgctttaat
    30601 cgcaaattgc gatctaaatc aaattaatcg gttaaagata accgcagcgg ggccgacata
    30661 aactctgaca agaagttaac aaccatataa cctgcacagg acgcgaacat gtcttctcat
    30721 ccgtatgtca cccagcaaaa taccccgctg gcggacgata ccactctgat gtccactacc
    30781 gatctgcaaa gctatatcac tcatgctaat gacacttttg tgcaggtgag cggctttacc
    30841 ttgcaagagt tacaagggca gccgcacaac atggtgcgtc acccggatat gccaaaagcg
    30901 gcgtttgcgg atatgtggtt caccctgaaa aaaggggagc cctggagcgg catcgtgaaa
    30961 aatcgccgca aaaatggcga ccattattgg gtgcgggcca atgcggtacc gatggtgcgc
    31021 gagggaaaaa tcagtggcta tatgtcgatt cgtacccggg cgacggatga agagattgcg
    31081 gcggtggagc cgctgtacaa agcgttgaac tccggacgta ccagtaagcg tattcataaa
    31141 ggcctggtgg tgcgtaaagg ctggctgggt aaactgcctt cattaccgct tcgctggcgg
    31201 acgcgtggag tgatgaccct gatgtttatc ttgctggcgg ccatgctttg gtttgttgct
    31261 gccccggtgg tgacgtattt cctctgtgtg ttagtggtat tgttggcaag cgcttgtttt
    31321 gaatggcaga ttgtgcgccc gatagaaaat gtcgcccgtc aggcactgaa ggtggcgacc
    31381 ggagagcgta atagtgttga gcacctgaat cgcagcgatg agctggggct gacattacgc
    31441 gcggtagggc agcttggcct gatgtgccgt tggctaatta acgatgtctc aagccaggtg
    31501 tccagtgtca gaaatggcag tgagacgctg gcgaaaggca ccgatgaact gaacgaacat
    31561 acccagcaga cagttgataa cgttcagcaa acggtggcga ccatgaacca aatggcggcg
    31621 tcggtgaaac agaactctgc cacggcgtcg gctgccgata aactttctat caccgccagt
    31681 aatgcggcag tgcagggtgg ggaggcgatg accacggtga tcaagacaat ggacgatatc
    31741 gccgacagta cctagcgcat tggcaccatt acttcgctga ttaacgatat tgcgtttcag
    31801 accaatattc tggccctgaa tgcggcggtg gaagcggcgc gtgccggcga acagggcaaa
    31861 ggttttgcag tggtggcagg ggaagtgcgt catttagcca gccgcagcgc taatgctgcc
    31921 aacgatattc gcaagctgat tgatgccagt gctgataagg tgcaatccgg ttcgcagcag
    31981 gtacacgccg ccggacggac gatggaagat attgtggcac aggtgaaaaa cgtcacccag
    32041 ttgatcgccc agattagcca ttcaacgctg gaacaggccg atgggctttc cagcctgacc
    32101 cgtgcagtgg atgagcttaa cctgatcacc cagaaaaatg ccgagctggt ggaagagagt
    32161 gcgcaggtgt cggcgatggt gaaacaccgc gccagccgac tggaagacgc ggtgacggtg
    32221 ctgcattaat cggtatgccg gatccggcgg ttggagcaca atgcctgatg cgatgctggc
    32281 gcatcttatc aggcctgcgg ggtgtgtagc gggtgtaggc cggataaggc gcgtcaagcg
    32341 tcgcatccgg caattgcacc gcgccactgg cggatgcggc gattaatcca gttgcgtaat
    32401 atcaaaagcg gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cactgatatc
    32461 gctctggttg acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg
    32521 cgggttctgg cgcagcgccg cgccaacgca acgcgctttg atgcgttctt caatcatctc
    32581 cacttgttcg cggttttctt ccagccactg cgcgccttgt tcggtcagcg caatctgctt
    32641 cttaccacct tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt
    32701 cgggtagatg acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt
    32761 aatcaattcg taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa
    32821 ttcaccgtga ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca
    32881 acattgaccg tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc
    32941 tttgcagcag ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg
    33001 gctcatatca gcctcctttt gcttgattag atatatctaa tttatatctg atgtgagcat
    33061 tactgcaagt acaaaaatga tttttaaacg tggaagcacc tcacatctag gccggataag
    33121 acgttcacgc cgcatccggc agcattgcta tggcactgaa tgccttcaca tacttcagac
    33181 atatcagttc tacaaatcgc ttgcatttat catgattaac aatcattatc atttgcgaat
    33241 tttatttaga tatatctgat taccatcacg aaggcgataa caatgaataa caccccccgc
    33301 tacccgcagc gcgttcgcaa tgatctgcgc ttccgtgaac tgactgtgct acgcgttgag
    33361 cgcatcagcg ccggttttca gcgcattgtc cttggcggcg aggcgttgga cggttttaca
    33421 tcgcgcggct ttgacgatca cagcaaactc ttctttcctc aacctgacgc tcactttgtg
    33481 ccgccaacgg taacggaaga gagcatcgtc tggccggaag gaccacgccc accgtcgcgt
    33541 gactatacgc cactgtatga cgaactacgc catgaactgg cgatcgattt ctttattcac
    33601 gacggtgggg tcgccagcgg ctgggcgata aacttacggt ggcaggtccg cgcggttcgc
    33661 tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg cgatgaatcc ggaatgcccg
    33721 cactgcgccg ccgcctggaa acgttgagca aacttgccgt taaaccgcaa gttagtgcgc
    33781 tggttagcgt gcgggataac gcctgtcagg attatctcgc gcaccttgat ggttttaata
    33841 tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg tctggcgcag atgcaaatcc
    33901 ctgccgatga ttacttcatc tggataaccg gcgaaggtaa agtcgttaag aatttaagcc
    33961 gccgctttga agcggaacag tatgacccac agcgggtacg tgcagcggct tactggcacg
    34021 caaagtaact gatatataaa aagaaaaagg ctgacgattt ctcgtcagcc tttgctatat
    34081 ctggtggccc ctgctggact tgaaccagcg accaagcgat tatgagtcgc ctgctctaac
    34141 cactgagcta aggggccatg gtagcggatt ataaagtaac tccgtgccgc aatccagcca
    34201 ttaccgcgcg cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg
    34261 tgatgttaga aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg
    34321 ttttgcggta tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatccg
    34381 gcaaatcgct tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg
    34441 caggaggcac agcatctgct ggattatcgt tgtggcatca ccaaactggt agaccgtcca
    34501 acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt
    34561 gaaaaaattg aagattatca gccgcaggcg ttggctattc tgggcaaaca agcatatgaa
    34621 cagggattca gctagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg
    34681 cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg tttcactgga gaaactggtt
    34741 gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg ggcgataa
//
LOCUS       DBBBSB010000032        34447 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.032, whole genome shotgun sequence.
ACCESSION   DBBBSB010000032 DBBBSB010000000
VERSION     DBBBSB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.032"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..334)
                     /locus_tag="ACIUS0_001534"
     CDS             complement(<1..334)
                     /locus_tag="ACIUS0_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219326.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            496..552
                     /gene="ysgD"
                     /locus_tag="ACIUS0_001535"
     CDS             496..552
                     /gene="ysgD"
                     /locus_tag="ACIUS0_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGB0219327.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="ACIUS0_001536"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="ACIUS0_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGB0219328.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="ACIUS0_001537"
     CDS             complement(1695..2075)
                     /locus_tag="ACIUS0_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HGB0219329.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2469)
                     /gene="yigG"
                     /locus_tag="ACIUS0_001538"
     CDS             complement(2089..2469)
                     /gene="yigG"
                     /locus_tag="ACIUS0_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HGB0219330.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="ACIUS0_001539"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="ACIUS0_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGB0219331.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /gene="yigI"
                     /locus_tag="ACIUS0_001540"
     CDS             complement(3506..3973)
                     /gene="yigI"
                     /locus_tag="ACIUS0_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGB0219332.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="ACIUS0_001541"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="ACIUS0_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGB0219333.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="ACIUS0_001542"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="ACIUS0_001542"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGB0219334.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="ACIUS0_001543"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="ACIUS0_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGB0219335.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="ACIUS0_001544"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="ACIUS0_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGB0219336.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="ACIUS0_001545"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="ACIUS0_001545"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGB0219337.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="ACIUS0_001546"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="ACIUS0_001546"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGB0219338.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="ACIUS0_001547"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="ACIUS0_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGB0219339.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="ACIUS0_001548"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="ACIUS0_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGB0219340.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="ACIUS0_001549"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="ACIUS0_001549"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGB0219341.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="ACIUS0_001550"
     CDS             complement(14628..15443)
                     /locus_tag="ACIUS0_001550"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGB0219342.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="ACIUS0_001551"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="ACIUS0_001551"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGB0219343.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="ACIUS0_001552"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="ACIUS0_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGB0219344.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="ACIUS0_001553"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="ACIUS0_001553"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGB0219345.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="ACIUS0_001554"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="ACIUS0_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGB0219346.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="ACIUS0_001555"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="ACIUS0_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGB0219347.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="ACIUS0_001556"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="ACIUS0_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGB0219348.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="ACIUS0_001557"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="ACIUS0_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGB0219349.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="ACIUS0_001558"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="ACIUS0_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGB0219350.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="ACIUS0_001559"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="ACIUS0_001559"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGB0219351.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="ACIUS0_001560"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="ACIUS0_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGB0219352.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="ACIUS0_001561"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="ACIUS0_001561"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGB0219353.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="ACIUS0_001562"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="ACIUS0_001562"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGB0219354.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="ACIUS0_001563"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="ACIUS0_001563"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGB0219355.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="ACIUS0_001564"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="ACIUS0_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGB0219356.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="ACIUS0_001565"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="ACIUS0_001565"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGB0219357.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="ACIUS0_001566"
     CDS             31577..32191
                     /locus_tag="ACIUS0_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGB0219358.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="ACIUS0_001567"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="ACIUS0_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGB0219359.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="ACIUS0_001568"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="ACIUS0_001568"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGB0219360.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8109 a         8699 c         9302 g         8337 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgcactg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgctctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg taacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgataa tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgt atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt aatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc agccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctttgtagcg ggaaagcgta ttatgaacac
    34441 cccgcgc
//
LOCUS       DBBBSB010000033        33535 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.033, whole genome shotgun sequence.
ACCESSION   DBBBSB010000033 DBBBSB010000000
VERSION     DBBBSB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33535
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.033"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACIUS0_001569"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACIUS0_001569"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGB0219361.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACIUS0_001570"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACIUS0_001570"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGB0219362.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACIUS0_001571"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACIUS0_001571"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGB0219363.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACIUS0_001572"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACIUS0_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGB0219364.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACIUS0_001573"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACIUS0_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGB0219365.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACIUS0_001574"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACIUS0_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGB0219366.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACIUS0_001575"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACIUS0_001575"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGB0219367.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACIUS0_001576"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACIUS0_001576"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGB0219368.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACIUS0_001577"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACIUS0_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGB0219369.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACIUS0_001578"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACIUS0_001578"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGB0219370.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACIUS0_001579"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACIUS0_001579"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGB0219371.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACIUS0_001580"
     CDS             8212..9075
                     /locus_tag="ACIUS0_001580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGB0219372.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACIUS0_001581"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACIUS0_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGB0219373.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACIUS0_001582"
     CDS             10411..11028
                     /locus_tag="ACIUS0_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGB0219374.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACIUS0_001583"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACIUS0_001583"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGB0219375.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACIUS0_001584"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACIUS0_001584"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGB0219376.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACIUS0_001585"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACIUS0_001585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGB0219377.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACIUS0_001586"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACIUS0_001586"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGB0219378.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACIUS0_001587"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACIUS0_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGB0219379.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACIUS0_001588"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACIUS0_001588"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGB0219380.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACIUS0_001589"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACIUS0_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGB0219381.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACIUS0_001590"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACIUS0_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGB0219382.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACIUS0_001591"
     CDS             21859..23568
                     /locus_tag="ACIUS0_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGB0219383.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACIUS0_001592"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACIUS0_001592"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGB0219384.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACIUS0_001593"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACIUS0_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACIUS0_001594"
     tRNA            27625..27719
                     /locus_tag="ACIUS0_001594"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACIUS0_001595"
     CDS             27991..29202
                     /locus_tag="ACIUS0_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0219385.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="ACIUS0_001596"
     CDS             29614..30657
                     /locus_tag="ACIUS0_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGB0219386.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="ACIUS0_001597"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="ACIUS0_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="ACIUS0_001598"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="ACIUS0_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            33237..33494
                     /locus_tag="ACIUS0_001599"
     CDS             33237..33494
                     /locus_tag="ACIUS0_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219387.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8402 a         8815 c         8263 g         8055 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 tgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccgg
//
LOCUS       DBBBSB010000034        31860 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.034, whole genome shotgun sequence.
ACCESSION   DBBBSB010000034 DBBBSB010000000
VERSION     DBBBSB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31860
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.034"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            152..907
                     /locus_tag="ACIUS0_001600"
     CDS             152..907
                     /locus_tag="ACIUS0_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HGB0219388.1"
                     /translation="MKNSINFILQGKGGVGKSFTTAILAQYFIDEKNMDNVVVGDTDP
                     VNTTTAKVKRLNADLIQITENSKVVQSKFDPMFESMLTNTQNTFVIDNGASTFLPLIQ
                     YFNDNCVMDMFNEVEQEVYIHTIIVGGQAQADTIEGFEDLVKLVKGTKVKIIVWINEF
                     QGVPALENIPLIKTKFIEKNKDVIAGVVVIQDRKSDAFASDIKNLTEKSLTLKEALES
                     EFFGLMAKSRLKRVFNDIYVQLDSIYNMQHTEA"
     gene            957..1634
                     /locus_tag="ACIUS0_001601"
     CDS             957..1634
                     /locus_tag="ACIUS0_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219389.1"
                     /translation="MINDKSFNIENIISDIFKETRLKISKDDPVLSIILMHEKILEHA
                     LTQLKNSNQIATERLSHDISSIRDAINALPDAIDEKTSELQHAAVALHDEFQESKGEI
                     KGSLEEARINATEKLAESAKELQLNITKVAEKTTETIESANKIISAIDTNLAEINKKA
                     LANYVNDIRSLEKKGESISKNIDTAINNAFKSSVKSFKFYCGAALFISTVLQFTMWGF
                     FLYKLLT"
     gene            complement(1953..2255)
                     /locus_tag="ACIUS0_001602"
     CDS             complement(1953..2255)
                     /locus_tag="ACIUS0_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HGB0219390.1"
                     /translation="MDTFDSEKFLSELDVLSKESMPGCGLVFELYQRRLSAAIDKVVA
                     GLPEEQHAQAFELARQEFDYLSAEEIAEEIRRDSERGYCCHGFDRDCCPLGCGDLP"
     gene            complement(2331..2564)
                     /locus_tag="ACIUS0_001603"
     CDS             complement(2331..2564)
                     /locus_tag="ACIUS0_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219391.1"
                     /translation="MSSEVIKQIQKIQDRGIIIYSKFRAAEFDQDDVYRESYFLVVEF
                     NELIAENIIHDEKLVDQTACILHELRRIAIEGK"
     gene            3116..3775
                     /locus_tag="ACIUS0_001604"
     CDS             3116..3775
                     /locus_tag="ACIUS0_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HGB0219392.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCVIKKGKTACFLETDSQGS
                     IKDFIEERQANASLSEIPYYECYTDIPAMVQKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADIVLTFVEPGSGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCATNPSDSEASEL
                     RNQLNDDPDWLPVPRQRIYMRTAHKKAYNCGMGVHEYNDKRGNKARGEIELLLKETNI
                     I"
     gene            3865..4167
                     /locus_tag="ACIUS0_001605"
     CDS             3865..4167
                     /locus_tag="ACIUS0_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219393.1"
                     /translation="MAKLPKKINQEIKALDLRQFVSSAAQKPASASTVTRINLTMTDT
                     DLDIAQEFQNEYGASRAEVIRTALAALRLISAEERRMLFDDIRKNSPKAGRPTVNK"
     gene            4203..4487
                     /locus_tag="ACIUS0_001606"
     CDS             4203..4487
                     /locus_tag="ACIUS0_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219394.1"
                     /translation="MLMTAIKSVFNFYMLCVQPLLGLFLVGYSIWSFSGDGICTADKV
                     IACDISIYLEPAFYLLIGSVVLFVWYIAWQTERSKEEDFKSSIKPEDFVK"
     gene            4649..4930
                     /locus_tag="ACIUS0_001607"
     CDS             4649..4930
                     /locus_tag="ACIUS0_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219395.1"
                     /translation="MSEIYCIAITVIIVGYVYLMISVITAPQKLFPILQLITLIGISS
                     LFSFMSFKSESMWTGEPELLVAIMFGTFSATLIFYMFKILIRAFDSLTK"
     gene            complement(5996..7018)
                     /gene="repA"
                     /locus_tag="ACIUS0_001608"
     CDS             complement(5996..7018)
                     /gene="repA"
                     /locus_tag="ACIUS0_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HGB0219396.1"
                     /translation="MASLNRPSLHWSELSPEEQILFWEDYESGRATSFLVEPEKRSTK
                     RRRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQYTLRMHMSLHPF
                     YVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDSKG
                     NVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKMLGV
                     DLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRRKK
                     AAERKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTQERLEQLVARDLRQLELAL
                     TASPPH"
     gene            8162..8446
                     /locus_tag="ACIUS0_001609"
     CDS             8162..8446
                     /locus_tag="ACIUS0_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219397.1"
                     /translation="MSQAKANLKKLRHHCYIPPKQKISRLYYVEKALSSPWIYRERPA
                     CRAELLLTHDTLLVIYGLAKSGLIDRSEESLNREIKGVRKALAAGRFRWS"
     gene            8702..9193
                     /locus_tag="ACIUS0_001610"
     CDS             8702..9193
                     /locus_tag="ACIUS0_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219398.1"
                     /translation="MKHKNFIPKWYDSTLESIPHQDVNTIHSIIDEHHKLWKIQRLIR
                     LVILLIITAFGLFSAFLNRENIFYIFTVGFSLGLILYIIAAIDSICHFLPNPLSDHVI
                     IDRETIQFLIVLTSHYPDVQQELFNRLLSGKKLTIHDEREIIDICSRRSATYHLTRSN
                     IPK"
     gene            9272..9853
                     /locus_tag="ACIUS0_001611"
     CDS             9272..9853
                     /locus_tag="ACIUS0_001611"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGB0219399.1"
                     /translation="MTLISQCAPGVSPETMRAIIMTESGGNPYAIANVTDGGSKYFTT
                     EEEAVNHAKKLTTDKKNFSAGLGQINSRNFQNLNLTNESVFSPCANIRAAAAVLKTCW
                     DKYETKGGDQQKILRDAMSCYYSGNNKRGYVKESNGKSYIDIIEEKAGIHTHYDIPAI
                     KSYSETSSHLMVSSDNHDKWDVFGDFRTQDFKE"
     gene            9932..10237
                     /locus_tag="ACIUS0_001612"
     CDS             9932..10237
                     /locus_tag="ACIUS0_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219400.1"
                     /translation="MHKSINLYRMFFLFVCAFFIASNPALAAGVGGLNKGTNFIGELR
                     LWAYGFVGGVALLHILWKVFQAYTEMDSWAGVLKSLMYAAIAGGCVVAAEACYQIFK"
     gene            10292..10615
                     /locus_tag="ACIUS0_001613"
     CDS             10292..10615
                     /locus_tag="ACIUS0_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HGB0219401.1"
                     /translation="MDKEEIKAAEELKAIFLTYNGLNRPAMIKGMPIIPFILCLLALM
                     VSFFVGILTIDFYGLIIPSIIIGVMIAIKQMCADDPNAMSARALKFKGLCLKGFRSNN
                     VLKVE"
     gene            10617..13046
                     /locus_tag="ACIUS0_001614"
     CDS             10617..13046
                     /locus_tag="ACIUS0_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HGB0219402.1"
                     /translation="MSIKKITNNFIPPYHSHIHENIIWLEDKKLLLTFLIEGIPYESL
                     TDDMILNNFNGQKETLLGLCKSERVFLWKHLVKRESPMDARYKFPGNPFLQRLGDFYC
                     DTFNGERSFRTDYFLTIGIPYDDIDAGEKKAKDIIRQIETGFKDYNIYTLGISDGGIS
                     DICSNYLNYLLNHDFEKIPLSATKISESIKNSKFYFSFDTLEIKNRTGHDNKFCSNYI
                     VKDVPRGSQIGQWNFLLSLPFEFILTQSFIPDSLSKSSKKIEQQLNKLTSVKDAGKTQ
                     HTELHMAQEAIINQETVFGSWHSVLSVFGNTPDEASDNGSEVAAEFVTSGRGFRFVRA
                     ASEAPYAWFSHLPASKLRPLATQRTLTNVCCLMPLHNHSYGKKSGNPIGDGSAIMPLK
                     TVTDGLYWFNTHYSAPGRNVTGQMIAGHGMLLGATGAGKTTFEMMAAAFIQRFNPDMF
                     VIDFNRSTELAIRMFGGQYFSLSEGVYSGLNPFQIGDNDDPELMAFLKEWVKRCAVNN
                     DGSDCSDSEAEEIDRAVEMVMKLERRQRRFARLQSRIQSPELNVRLSKWWDNGALAWA
                     TDSEENKFDPNQYSKIGFDTTVILETVNQRDHPACEVILSVLFFYKKRMQKGGKLMLS
                     IVEEFWKPCNYPMTQALIKASLKAGRMKGEMMWLTSQSPEDAINCQIFAALVQQTPTK
                     ILLPNPDAKYSGYKEIGLTEKEFRQLKKLKLESRTMLIKQSGSSVFAKMDLVGFDDFL
                     PVISCSMKGIKLCEKIREEIGSDDPELWYPPFIEGIYQIDDVIREANSDIPEVWLPFM
                     MKRLKDKLPEK"
     gene            13276..13944
                     /locus_tag="ACIUS0_001615"
     CDS             13276..13944
                     /locus_tag="ACIUS0_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HGB0219403.1"
                     /translation="MKKTLTALFLGATLTGYSSVASSVGGVIVKDPGSIAKTVEVIKK
                     AADQIAELKKQVDLATSQLEAYKQEVLDTKRRLEGITDYSAIFGSAESYMKDFWEDMK
                     KELTDADIRSMATKYGFDTKEYDRIKRQYESKFEEITKYEAMSKDLEKSAEKIKATQK
                     AFSKADTPQKREELQNNILLETAAMQLKIAQNEAEINRMARERKLREEAALIKYTEDN
                     FSFN"
     gene            14038..14382
                     /locus_tag="ACIUS0_001616"
     CDS             14038..14382
                     /locus_tag="ACIUS0_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219404.1"
                     /translation="MFKKLLGGFILAFTFLLSGCGGDNGELAGTYIEKNPDNSDAPQK
                     WIIKYEKDNYYTQIWVEKDKKGNEHNGTTNSFLIRDGDWLAGKEKHFIRVIDKNTLQL
                     RDDENALYIRTN"
     gene            14448..15494
                     /locus_tag="ACIUS0_001617"
     CDS             14448..15494
                     /locus_tag="ACIUS0_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HGB0219405.1"
                     /translation="MASNDSAFAKANFEIVQPMFNELSSIIQNSVTTNFQTIMQLVSP
                     LFGAAVILYGVFFAWEIMYTRKDDVMMESVKRMGVLTLVFAMSTAGGMYLSYIVPFVQ
                     GSGQEVASRLIGQDGASTGAVIDGLLNRLIDLGHEQFKVFQKADGWFDSMGAWIVFIV
                     KAILLALAGGGFVIYCAAYLLMAEMMVGILLSIGGVFISFSAWPTTRNMFTAWVGSCF
                     NYIFLNIAYACLFKVLIQYINNYINTNSDDITKNIWSVLCVAVVFLIGKFLLAQISTL
                     ISTLTGGVGINGLTSATGDVIKSLRGAGNSTRNQLGLGFNKAAQDARARNSEYAAAAK
                     DYLSSRFGGNIKAG"
     gene            15695..16441
                     /locus_tag="ACIUS0_001618"
     CDS             15695..16441
                     /locus_tag="ACIUS0_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HGB0219406.1"
                     /translation="MGLKEYFKSSKAEARKTPAQKETDKKIEKNVLDAAKEFARDRDN
                     ELKRSLRITRFALFFSSASAAAMAVALAVMAPLKTTEPFLLRVDKTTGYTDVITPFSQ
                     DSGTYDEAVSRYFLTRYLENREGYEWYTIQNMLNTVELMSSSQVFAEYNNMIHGPLSP
                     LYKLKKSYKILVRVQSITFLDENTVQGRFVKAITDPDGKPAPGYTPTQWIATIKFVWL
                     KDKISTEAERQINPFGLDVLSYRVDPEVVK"
     gene            16486..17235
                     /locus_tag="ACIUS0_001619"
     CDS             16486..17235
                     /locus_tag="ACIUS0_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HGB0219407.1"
                     /translation="MTCHAAKTPASAGFDRRVQQIVYNPNDVTIVKTKAGEVTLIELE
                     EGEIIKSEDTGLAIGDPEAWDPAVRGNTIFLRPKAAEPDTNIAIVTNKRIYSIQLVST
                     DQNPTYLLRYIYPKPPEPVKPNVMTGRSRVVAPCTEGNIVNGRYEVRGSRKIKPEAIW
                     DNGRLTCFRWTGAADLPVVFRILPDGKEQLVNYHMDKNIMVVHEISPAFVLRLGGEVM
                     EVRTNRHIPRTWNDSGTLTGETRVEINNDEK"
     gene            17225..18448
                     /locus_tag="ACIUS0_001620"
     CDS             17225..18448
                     /locus_tag="ACIUS0_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HGB0219408.1"
                     /translation="MKNNNDIVIEKLDEIDTQEADTDAQNRNFIHSGKKQGNKKAKAV
                     LILAGFAFVTVVILTSVLSKVFSSSGEEETTETATTKAQAEVYAQNTQAKNLDDLKES
                     VARSIEEVKNNVNSEEEQAREEAPPENKENKGVTLPAENEADTGYTQNNGDAPPTPEQ
                     RRLMGSLMVDDSRITQSQPGSSGQKTAGATPASDNTNVSGDGDFLQGADFADGSVSRI
                     KNRRFLLSAGTTFPCVLKTMIVTTYPGVTLCQLTKDVISDDGKTVLIRAGAQLQGEQT
                     KAITQGMARVFVNWTMVKDRNVRVRINALGADSLGASGLPAWVDTHFWERFGGALMLS
                     LIEDTLAAGTSRLSRENNSDFTMNNTTGTSSELAKTTLDNSINIPPTGYVNQGEMLTV
                     IVPRNIDFSSVYGVR"
     gene            18448..19461
                     /gene="virB11"
                     /locus_tag="ACIUS0_001621"
     CDS             18448..19461
                     /gene="virB11"
                     /locus_tag="ACIUS0_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HGB0219409.1"
                     /translation="METINMFLESTGVQPFLEAEGLTEIIVNRPFEIITEGVNGWEYH
                     EAPGASYSDLLDLSIAINKYNNSADPLDALHPIKSLVMPGGERMQIIMPPACEKKCIS
                     FTIRKPSLRRFTLEDYVSSNRFSNVRIVNQANLSALSASQQTLLDLLDNTRCAPRTWI
                     SFLEAAVAARMNILVVGGTGSGKTTIAKTIADLFPRDRRIITIEDVHEMTLPNHRNHV
                     HLFYKQGGVQPRYLIEAAMRMKPDHIFLAELRGDEAWSYLEALNTGHEGSVTTIHANS
                     ALDAISRLASIVKQSTVGLTLDHSFILKTIKTSIDIVVFFRHTHMTEIYYNPMEKNQL
                     LAA"
     gene            19630..19893
                     /locus_tag="ACIUS0_001622"
     CDS             19630..19893
                     /locus_tag="ACIUS0_001622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219410.1"
                     /translation="MRRRMPQLARATASLRRPGLTWTGRCTARVSVSELRAQLSGEKP
                     HDNGKSDEEEETYSACTYSLADGPDSLRLAGEFYTGENCLAAN"
     gene            20027..21814
                     /locus_tag="ACIUS0_001623"
     CDS             20027..21814
                     /locus_tag="ACIUS0_001623"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGB0219411.1"
                     /translation="MFVVVREKKTVHGNARLANDRDLADSGFFPDEKELKNQKYPSLL
                     IGKMPEHKGRFKSRYLQYSGQQFLMLYAPTRSGKGVSIVIPNGIWYTESMVFLDIKLE
                     NFLETAGYRKNVLNQEVYIFSPDGYAREIGGETVLTTHRYNPLYYIRRDPVYRFGDLE
                     KIAVILFPMTGGENDTWVESSKNVFISLVLWLLDTELEGKFKVNMNSVMKLSIPADGS
                     SLAKWMLNEIRSRGNEVLTERWKAYHALPEEEKQGKTPSTYPLSDDTIRLFRQFAAKE
                     PEQQESIMLDFNRTMSMFANPVVAAATEDNDFDMRDVRRKKMTIYYGISPGALKQYQR
                     LTNLFFSQLLNENVRTLPEHDATLKYQCTLMLDEFTSMGRLDVVQVSLAFTAGYNLRF
                     VFILQNREQLFDEKHGYGKMGGNTILKNCAVEIFYPPKKVDESVKDVSETIGYYDFVR
                     TQISRTTGKNPSTTRNKIIEKRAVMLPQEITDLRDQKFSVKGKKTSFSLPQIVISEFC
                     RPFIAHKIVSFDQNKESFFVNARKYSRKNIIDTPVLDIPQNEVVRQLMLQREREEAWK
                     ATQPAFDKDDFDDDKEYFPGDDDGGGGGD"
     gene            22054..22383
                     /locus_tag="ACIUS0_001624"
     CDS             22054..22383
                     /locus_tag="ACIUS0_001624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219412.1"
                     /translation="MKSRLLISITLLSALTFTLSASAKIKGLSAAGDPCEVLVCMAEK
                     VQGAPQPGCISVNQRFFNIRVYTPFYNPGATYGARQNFLYTCSGSTVNQATLNMIMQR
                     YGRLFSG"
     gene            22460..22798
                     /locus_tag="ACIUS0_001625"
     CDS             22460..22798
                     /locus_tag="ACIUS0_001625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219413.1"
                     /translation="MSFIKINRQREFMERSNSPRLVLRKEKRRNGIVNCYLSKTVRGD
                     DTFITIELNPEHNKIRLKTTNSPEGHKMSRGCFSLTKVFEYIFHENLHEDRIIIELEK
                     KENGWWYGNY"
     gene            22785..23042
                     /locus_tag="ACIUS0_001626"
     CDS             22785..23042
                     /locus_tag="ACIUS0_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219414.1"
                     /translation="MEITKNRETRFVTLASDFNNIKEIYSDDELNRLLEAVKNCPTII
                     VLGCANKGKETPLMASCLRTTPERISVEEFNEIKAISSKQK"
     gene            23137..23214
                     /locus_tag="ACIUS0_001627"
                     /pseudo
     CDS             23137..23214
                     /locus_tag="ACIUS0_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="micrococcal nuclease"
     gene            23230..23634
                     /locus_tag="ACIUS0_001628"
     CDS             23230..23634
                     /locus_tag="ACIUS0_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGB0219415.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDLSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            23694..24014
                     /locus_tag="ACIUS0_001629"
     CDS             23694..24014
                     /locus_tag="ACIUS0_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219416.1"
                     /translation="MNIHLFSEVLFCVWVIALIVILFIVVKYYRRVHYRLNSLSETIK
                     RTQGGVNKRISENRELLELIKNQHPEILDEYPWVSGWLDSQEKFLVALADKSGIDINK
                     SGLI"
     gene            24041..24628
                     /locus_tag="ACIUS0_001630"
     CDS             24041..24628
                     /locus_tag="ACIUS0_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HGB0219417.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPELDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDDFDMV
                     SQEILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLM
                     SREESLLLHTIQSERLNTDCSLFKRQPSIESALVI"
     gene            24653..25228
                     /locus_tag="ACIUS0_001631"
     CDS             24653..25228
                     /locus_tag="ACIUS0_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGB0219418.1"
                     /translation="MQYALVDVLERKFLLDALEFGVLKDWKENPVKELPDIDESVHPF
                     HVCYGGYLLNPGVSDSDISRKIKDQTGFWLAAIDDTRMDCHSIAYYDIHTLPLILCGH
                     QKIVPFAALIKADECIISKIASYSGFAVTAFLRIKDQDIATNILNREGIFAFNGCERR
                     FRQPVSEDNWQQAVSEERAIRCANRLIQCKG"
     gene            25233..27395
                     /locus_tag="ACIUS0_001632"
     CDS             25233..27395
                     /locus_tag="ACIUS0_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HGB0219419.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SENNDDSAFEQLCKIRTGETLTTKEVVVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     VIKKLLKDKDKDKKDEHGGIGTPATRAAILEALKKRNYITLEKGKLIPTDTGCALIDA
                     LPGIAVNPDMTALWSEKQSAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPTAP
                     AGQSPRLSSPCPSCGKAIIIRPKGYFCTGCEFKIWKNFSGKALSDKQVETLVTKGITG
                     ELKGFVSSKTNKNFSAKVKLVDKTAGKLGFEFTHKKSI"
     gene            complement(27566..30853)
                     /locus_tag="ACIUS0_001633"
     CDS             complement(27566..30853)
                     /locus_tag="ACIUS0_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HGB0219420.1"
                     /translation="MLVRISGGNKGVCEYLEKGHKQGREYTRDEIDHRLVLDGDLALT
                     ESVIDAIPDNGQERYMHITLSFFEDDVSESKLHDVVQEYKSLMMNAYHEDEYNFYAEA
                     HLPKIKQMPDHKTGEMVERKPHIHIVIPEVNLVTGNKLNPGGVINHYLKHLDAIQEHI
                     NNKYNLVSPKDGVRVSDHNHANVLSRAKGDFYRERHSEFKTTVFDALDTENIRTEKQF
                     SDYLSRFGEVKEYNKGKSDRYFGVKLEGDSKFTRLKSPLFSRQYISDRTIPLLKPTKT
                     QIESHLKEWKSSISHEIKHIHPAGQKIRKEYAALAGEDKQMYLKKVINRYDEKHNLQR
                     QDRRQNSDQSGIKYNTGKFVSGKTIGLPRLPARELVYGLHGRSGGKSINKSQLLLQQN
                     EYGYLSTAGRQKVDPDRYVRRLQDRAGRRVVQSALESNIKAAIQRSDDLPFLKQIRTE
                     INAERYLSNLQTRFNIDPSKHTVTYGQDGSPRFRVGNRNMNASDFLTKHLNLEWDEAK
                     EVLLDIWQDQKDNKPFDAVITRVNLTRQQAKERFICLNEHKGWVNNEIKYKHNDNIAN
                     YRAELRGLLAIRNKQEREVARGYIIFKKLEREREINSIRKACFREINSYYHRWNPDRR
                     YDMALGDSLKKIIRNPVDGNSISSGNEKEVFSVVKRVQQSQQVQDYVKRELTDLVPHK
                     VSPKETRYLDPKTTQTVFTDKGNHVRVNGEITQDKAEAALLYAKEKFGGMLKLNGSEE
                     FKVACAMAAAEKGMNVILRPEKYHEMMQVRLQELKAEHGHENSVEAERQQQVAGQKPE
                     QEQEKRAEQVNSVEKDTQAKERTPEVAESVRNDDKSRDAVPEQPVKSETVSQKSTSET
                     SVVQEAEKQESVQQNVSAHSEEREQQPQEAQQQKPVTEKDVTTGQDKQTGDEQSKSAL
                     ATENPHQQNNASLQQLQEEAKVIASQHQEAQRLLNEAVENGAPKEEVDLLSKGVNSIS
                     ARAQTINEQIKAFEEQQGQKKAKSVEEKNENTTEKKKRTVNSEKAMEREIERISKQLA
                     KEMNIDKQTVKDTLKFIRFEDVNDLKAAMKNPETVEAVKREIVSYDNALKNERERERN
                     RDNTRSR"
     gene            complement(30847..31233)
                     /locus_tag="ACIUS0_001634"
     CDS             complement(30847..31233)
                     /locus_tag="ACIUS0_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HGB0219421.1"
                     /translation="MKKQDKGKKAVASTSKVERKHVISLRLTDEEYQSFHEYFTSLEV
                     SRSEFFRMVILNRLDDIPTLKQKRPVEYRELLRLFNKSSNNINQIAKKINTEYRNGFI
                     SESTYLRYLNVLVNIRDAFVAGINKC"
     gene            31593..>31860
                     /locus_tag="ACIUS0_001635"
     CDS             31593..>31860
                     /locus_tag="ACIUS0_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HGB0219422.1"
                     /translation="MKKIDSLLSPICEAIKETNSKSKQAVFNMYLSNILEAKSYYTWE
                     QITDYINNKTKTELAQKTYINMTERAKKRQKCTGQKDLKKSTNMI"
BASE COUNT         9457 a         6868 c         6774 g         8761 t
ORIGIN      
        1 aaaaaaaaat agatgaagac aacaaacaaa gaggaggcgt aaaaggattt ttttcaacaa
       61 tcagcgagaa caaaaacgaa atagatcacg atccaagcgc aacaaatgaa aaatttataa
      121 acaaatatct ttaaataaag gtgtatcata aatgaaaaac tcaataaatt ttatccttca
      181 aggaaaaggc ggtgtaggaa aatcatttac aacagcaata ctagcacaat actttattga
      241 tgaaaaaaac atggataacg tcgttgtagg cgacacagat cctgtaaata caactacagc
      301 taaagtaaaa agattaaatg ctgatttaat acaaatcact gaaaacagca aagtagtcca
      361 atctaaattc gatccaatgt tcgaatcaat gctaactaat actcaaaata catttgtcat
      421 tgataatgga gcatctactt ttttaccatt aattcagtat ttcaatgaca actgtgtaat
      481 ggatatgttt aacgaagttg aacaagaagt ttatatccat actattattg ttggcggtca
      541 ggcgcaagca gatacaatag aaggttttga agatcttgtt aaactagtaa aaggaacaaa
      601 agtaaaaata atagtatgga ttaatgaatt tcagggtgta ccagcacttg aaaacattcc
      661 tttaattaaa actaaattca ttgaaaaaaa taaggatgtc atcgctggag ttgtggttat
      721 tcaggacaga aaaagcgatg cctttgcttc tgacatcaaa aatttaaccg aaaaatcact
      781 aacattaaaa gaagcccttg aatcagaatt ttttggatta atggctaaat ctcgtctcaa
      841 gcgagttttt aacgatatat atgtgcaact tgattcaatc tataacatgc agcacacaga
      901 agcataagtg attatataac agccattatg ggctgttata tatttgagga caagttatga
      961 ttaatgataa atcatttaat atcgaaaaca ttatttctga tatttttaaa gaaaccagat
     1021 taaaaataag taaggacgat cctgttttaa gcataatact aatgcatgaa aaaatactcg
     1081 aacatgcact aacacaactt aaaaacagta atcaaatagc tactgaaaga ttatctcatg
     1141 acatatcttc aataagagat gcaatcaacg cattacctga cgctattgat gaaaaaacgt
     1201 cagaactaca acatgcagca gtagcacttc atgacgaatt tcaggaaagc aaaggagaaa
     1261 ttaaaggttc tctggaagaa gcccgaatta atgcaacaga gaaattagca gaaagtgcaa
     1321 aagagcttca attaaacatt acaaaagttg ctgaaaaaac aacagaaaca atagagtcag
     1381 caaataaaat aatatctgcc atagatacaa acctggctga aattaataaa aaagccttag
     1441 ctaattatgt taatgatatc agaagccttg aaaagaaagg tgaaagtata tctaagaata
     1501 ttgatacagc aattaataat gcttttaaat ccagtgtgaa atcatttaaa ttttattgtg
     1561 gtgctgcatt atttattagc actgtcttac aattcacaat gtggggattt tttctctata
     1621 aactcctgac ttaacttaaa tgccgcttcc agcggcatat ttggggtcag tttggatata
     1681 gagaattatt gtacgttcag cacaacaaac ttaatgctaa tgtatgcata cattatacat
     1741 taaccacaca tttaagactt aacacagtaa taatgtgtat tattacacaa taatacgcat
     1801 caggaagcgc gtcgtcagta ttggagaaaa acttacaaac gctgaccaca gccccagcga
     1861 ccgaacggta gtgagcgagt gggcgaggaa gaagaagacg accagatctg aatagcactt
     1921 gtgagaaggg gctacaagcc ccttcccgta tttcatggta aatcaccgca accgagaggg
     1981 cagcaatcgc gatcaaagcc gtgacaacaa tagccacgtt ctgagtcacg gcgtatttct
     2041 tcagcaatct cttccgcaga aagataatca aattcctggc gagccagttc aaacgcctgg
     2101 gcgtgttgtt cttccggcaa tccggcgaca actttatcaa tcgcagcact caaacgacgt
     2161 tggtatagct caaaaacaag accacaacca ggcatgctct cttttgataa aacatccagc
     2221 tctgagagaa atttttcact atcgaaagta tccatcatct tcaccttcat aagttaccag
     2281 acactatttc tgcatcagtt aataacaggg gccataagcc cctgttatat ttatttccct
     2341 tctatagcga ttcttctaag ctcatgaaga atacaagccg tttgatctac cagtttttcg
     2401 tcatgaatga tgttttctgc tattaactcg ttaaactcta cgaccagaaa atatgactct
     2461 ctgtaaacgt catcctgatc aaattctgcc gctctaaact ttgaatagat gataatgccg
     2521 cgatcttgaa ttttctgaat ttgcttaatc acttctgaac tcatttttaa tctcctgtcc
     2581 gtttggactt aagaaggaaa ggagccaatc acctttcgat gaaataataa tacatctatt
     2641 tagatgtatt tgcaaggttt ctggcctgaa aatgggtaaa ttatttgatg attgtcacgc
     2701 ttttatccgc gccagcggat gtaaggggcg aacgaaaata ccaggggagc acaggagcca
     2761 catcgccccg aagcgcggca acgccgctac tgttatcaac cggaacctgg gccaccttcc
     2821 cgccctgcca ggcccgactg actcacagac aacgatgacg ctgatttgcg ccgcagaatg
     2881 ccgcagttag cgctcgcgcc agcgagttta cgccgtccag ggtttccctg gacggggcgc
     2941 tgtactgcgc gtgtgagcgc cagcgagcta cgggtgcaac tcttctgtct acacagataa
     3001 ttattgtgta tcaatacaca ctataaaatt attgccagca cgcaattgtc cagatatact
     3061 ccacacgtaa ataattgtgt attaatacac accattaaac caggaggatt ttgtgatggg
     3121 aaaaatcatt acagttgctg ggcataaagg cggcattggc aaaagcaccg tgctttgctc
     3181 cttatgtgtg tgtgtcatca aaaaagggaa aacagcctgt ttcctggaaa cagattcaca
     3241 gggaagcatc aaggatttca ttgaggaacg tcaggctaac gccagtctgt cggaaatacc
     3301 gtattacgag tgctacacag atattccggc gatggtgcaa aaactggctg cccgttttga
     3361 ttatgtgttc gtcgatacgc ctggtatgaa atctccggca ttcgtaaaag cactgagttg
     3421 tgctgatatc gtcctcacat tcgtagaacc aggatccggc atcgagatca acacgctggg
     3481 acggctggtt ttcgacatta aaacagcaca ggccggagtc aatccgtcaa tgaaagcatg
     3541 gatcgttctg aataagtgcg caaccaatcc gtcagacagt gaagcgtcag aactacgcaa
     3601 tcaacttaat gatgatcctg actggcttcc tgttccacgt cagcgcatct atatgcgcac
     3661 tgcgcataaa aaagcctata actgtggtat gggcgtacat gaatacaacg acaaacgagg
     3721 aaacaaagcc cgtggcgaga ttgaattact gttaaaagaa acgaatatca tatagtgcgt
     3781 aatattacac atcgcattaa aaacggaaat tttcatttat aaataagtgt gtataattac
     3841 acactttata aaaaaggaaa ctttatggca aagctcccca agaaaataaa tcaggaaatt
     3901 aaggcgctgg atttacgcca gtttgtaagt tctgccgcac aaaaaccggc atcagcatca
     3961 acagtaacgc gtataaattt gaccatgaca gatactgatc tggatatagc gcaggagttc
     4021 cagaacgagt acggcgcaag ccgtgctgaa gttatcagga cggcactggc agcgttgcgt
     4081 ctgatttcag cagaagaacg ccggatgctg tttgatgata taaggaaaaa cagtcctaag
     4141 gccggaagac caaccgttaa caaatagcat gtattattac atgtcgttaa aggagtaaaa
     4201 atatgcttat gacagccatt aaatctgtat tcaattttta catgctctgt gtgcagcctt
     4261 tacttggatt atttctcgtg ggttacagta tatggagctt ttcaggtgac ggtatctgta
     4321 cagcagacaa agttatagcc tgtgatatat caatatatct ggaaccagca ttttaccttc
     4381 tcattggcag tgttgttttg tttgtatggt atattgcatg gcagacagaa cgctcgaaag
     4441 aagaagattt taaatcaagc attaagccgg aagattttgt gaagtgagat tatggttgtt
     4501 gataaattgt atgcagcatt agatatgcaa ctaaaattga ttgcgacggt gttataaaga
     4561 acattcctgt aagcacatat aacaaactaa tatacgccta taataaaagt tcattcttaa
     4621 cagatacaga acaagaggaa atgccattat gagtgaaata tactgcattg caattacagt
     4681 cattatcgtt ggttacgttt atctaatgat cagtgtaatt actgcaccac aaaaattgtt
     4741 tcctatactt cagctaataa ctctaatagg tatcagttcg ttattttctt ttatgtcatt
     4801 caagagcgaa agcatgtgga cgggtgagcc tgaattatta gttgcgatta tgtttggcac
     4861 tttttcagca acactgattt tttacatgtt taaaattttg attcgcgctt ttgattcatt
     4921 gactaaataa caaagctgaa taatcccgct gacaaagcgg gattatcttt actcttcatt
     4981 atataggata cgtgttgcag gcccgactct ggtgcgtatg tgcgcaattc acggctggca
     5041 cttttccgct tcctcgccca ctcgctcact accgttcggt cgctggggct gcggcgagcg
     5101 tttccgatac catgcaacaa tattgtgtaa taatacacat cctttttaat gccgcgccag
     5161 cggcatggaa ggcggcacgc tgtagttcca tgtggcgttg ctggttatcc gccgaagccc
     5221 cgcgccagcg ggtactgatt gacagatttc accttaccca tccccatcct gctaagccat
     5281 acccgctttc agccatgaca gagcttatgt gcgcggtcgg agtggtcccg acgagggttt
     5341 acccgaagtc ggggcgtatc tccgcgttag cgggccgtca gggccgctta cgagcgtgta
     5401 cttaatacat ccagccagaa gactgacagc gatgagattt cttagttaca acatccatta
     5461 ttgaaagcga tgttgttccc gccctttggg cgggggcggc ggcgcttttc agtgatgggt
     5521 tgggcgctgt ggctgagtat ttgcagcgtt tttttgtagg agggctggag gggctttgtt
     5581 tttcattagg cagaagtcca tatgcccgcc gcgaagcggt gggaggtact tttatattct
     5641 gagcggcgcg gcgttagccg ctgcaagcga aggcattttt gtgattatcc acagttgttt
     5701 caaagataaa tattttccct gcggcgaagc aacgcagggg attaaagaac aggggttaag
     5761 aatatattgc gggtttgatg ttctatgttt gctgctttct gaagttattt ctttgcgggt
     5821 ttttgtggat aaccgaaggc gtttttactc cgatacccta ttgcggacat caaaaaactg
     5881 tcagagcggg acaaaatgaa aaacggtcgt caaatatgag gaatttcatg aaatgcaggc
     5941 aacacgaaaa gccccgaagg ggctgaagag agtgccgatg tatcagacag ctaaactagt
     6001 ggggtggcga agctgtcagg gccagttcaa gctgacgtag atccctggct accagttgct
     6061 ctaatctttc ttgagtacac cagtatgcct catcaggtgg catacgtttc agaataaacc
     6121 gcgcaatttc gtttcgctgt tcatcatacg gcagttttgc caggcgatta gcacgtttgc
     6181 gctccgctgc tttcttacgt cgggacttga tggcatcgag agaacgctgc gcataccagc
     6241 gtttacgagc cgagtgaaca gagatttctt cgccctcgcg gataacgcct tctttaatca
     6301 gttgcagacg gatttcactt tctgcaaggc gcttacgttg ctgttcctga agtttcacca
     6361 gatcaactcc cagcattttc cagccagttt cggtgatcca gacgtatttc ggcagtcgct
     6421 ggcgggattc acggtcccat atcgtctttt ctgacgttcc caacgtgcca aaggaaacct
     6481 gctctgccag cagacgggaa aggcgcgata ctgtgactgc ggtttcagga ataacgtttc
     6541 ccttactgtc ttttgggctt atttcttctg ccaggcggga gacagacatg ccgacagtgt
     6601 gcaatccacg atcacaaaaa ctcacaagta caacccatat tgcgtcaagc agccgctgtt
     6661 tctccggtcg gaatttatat ttgcgaccga cactctgacg tttcagtaca taaaatggat
     6721 gcaacgacat atgcattctg agtgtgtact gaccagtgac cgggtctttt ttaacgagac
     6781 gattgtaagc gtggcccaac tgtccgccca gcgccttata gctgtcggga cggaaccagg
     6841 ctggattctc acactttggc ttagtggaat gttcaccacg gcggcgcttc gtgctccgtt
     6901 tttccggttc aaccagaaat gacgtggccc tgcccgattc gtaatcctcc cagaaaagga
     6961 tttgctcttc gggagacagt tcactccagt gtaatgaagg cctgttcagg gaagccataa
     7021 gcaatcgctt atggcagagc ttgattcgca cagtgcattt ttacaccgga cgatattgaa
     7081 taatgaggaa agcatcatgt atactgtctt ccaagggatt tcccctgccc ccattaaact
     7141 ttgatcggcc tctgccaaga aacggatgca aagttaatgg gggtttgttt tttatggctt
     7201 catggaatct ttcaactcct gccatacacc tgaatgcctt atggcgcatc ctacctttat
     7261 cccctatgac ggggaccaaa tttctcaaat ttaagcgaag atcaaaaagg atcaacaccg
     7321 aatgccgctt aatatacctg tagccctccg atttgaaaag tgtttgtcgt gacgctcatt
     7381 ccaccaatcc cgccaaatca gcacagccag cactgccgat gaaacaatca aaaagtattt
     7441 tttttcgcca cttctttaat ttttagaata ttagatcgtg aatgatctat tcaacgatct
     7501 atcataccaa tataaaagct gattttagaa ttttatgctt tttattttgc tttatgtggg
     7561 ataatagatc acccaaacca ccttgaacga tctattgtac tggtttaaag tctccatgtt
     7621 gaagttttcc gctatcagcc gaacgcccga acaaggcatt attcgtacgc tctttaacac
     7681 ccctggacag cggtaattgc ctgaagccct ggcatgagga ggattaattg tgtgcaaaaa
     7741 acagcacttg ctgacaatgt tcagtgtggg ctacgtatag agtgacgtgc cgcgccatct
     7801 gacaggtaac gactcatttt tacaattttc aacatagtta attcccttta tcaaactcga
     7861 ttccctgaaa gggggattaa ctgtgtacga aaagtacagg agataataat gagagatgat
     7921 aattatcttg atggattaat tgcctgaggt actgttgcaa tttcttttgc gggactgatt
     7981 ctgctaatcg tgagtaattt gaaaaacgaa gctgagacag aaaaaactgc attttatgaa
     8041 tgggccgatt cacactgtga aaaaattgaa aaaaatttaa atgttactga aaattcgaaa
     8101 acatttaagt gtgatgacgg aaaaatttac acaataaaat ggtgaaacag attaacatgt
     8161 tatgtcacaa gcaaaagcaa acctgaagaa attacgtcat cactgttata ttccaccaaa
     8221 acaaaaaata tccagactgt attatgttga aaaagcctta agttctccct ggatatatcg
     8281 tgaacggcct gcatgcagag cagagttgct acttacacat gacacactgc ttgttattta
     8341 tggcctggca aaatccggcc tgattgaccg ttctgaggaa tcactcaaca gagaaatcaa
     8401 aggggtccga aaagcgctcg cagccggacg tttccgttgg tcgtgagatg ccgcgccagc
     8461 ggcatgtaag gcggcactct gtacttaaat atggctttgc gggttatccg ccgaagcccc
     8521 gcgccagcgg gtactgtctt ctatattctc tggaactgcg ctcgcgcccc ctgctaagcc
     8581 caaaacgcac ggaaccaaca gctacacggt tgtttctggt cgaagaccga cactgctgaa
     8641 gcgatattat tcgaaagtta tcctactgcg caatgcccct gttttatttg aggaaaatgg
     8701 catgaagcat aaaaatttta ttccgaaatg gtatgattca actcttgagt caattcccca
     8761 tcaagatgtt aacacgattc acagtatcat tgatgaacac cacaaattat ggaagatcca
     8821 gagattaata cgtcttgtga ttctattaat cattacagca ttcggtttat ttagcgcttt
     8881 ccttaataga gaaaatatct tttatatatt tacagtcggt ttttctttgg gtttaattct
     8941 ctatataatc gctgctattg attctatctg tcattttctc cccaatcctt taagtgatca
     9001 cgtaataata gaccgcgaaa caatacaatt tctgattgtt cttaccagcc attatccaga
     9061 tgtgcaacag gaactattta acagactgtt atccggtaaa aaactgacga ttcatgatga
     9121 gcgagaaata attgatatct gctcacgaag gagcgcaaca tatcatttaa ccagaagtaa
     9181 tatccctaag taatctgacg ctgccaataa tccgttaaat taaactttca tcgttacctg
     9241 tcaaaaaatg tctatatccg caactgtgct tatgacactg atcagtcagt gcgcacctgg
     9301 tgtttctcct gaaaccatgc gtgccattat tatgactgaa tccggtggga atccctacgc
     9361 tatagcaaat gtcaccgacg gtggcagtaa atattttacc actgaagagg aggctgttaa
     9421 tcacgcaaaa aaactgacta cagataaaaa aaatttcagt gctggcctgg ggcagataaa
     9481 cagccgtaac ttccagaatt taaatttaac taatgaatcc gttttttctc cctgtgcgaa
     9541 tattcgtgct gctgcggcag tgctgaaaac gtgctgggat aaatacgaaa caaaaggggg
     9601 ggaccaacaa aagatactac gtgacgccat gagttgttat tactccggta ataacaaacg
     9661 gggttatgtg aaggaaagca atggcaaaag ttatatcgat ataattgagg aaaaagccgg
     9721 aatacatact cattatgata ttccggcaat aaagtcatat tcagaaacat ccagccattt
     9781 aatggtatcc agtgataacc atgataaatg ggatgttttt ggtgatttca gaacacagga
     9841 ttttaaggag tgagacacat ccattaattt atttattcct accatcttta acctcaactg
     9901 aattaatttt aactgattac ggagtaaacc cgtgcataag tctattaatt tatacagaat
     9961 gttcttttta tttgtttgtg ctttttttat tgcctcaaat cctgctctgg cagctggtgt
    10021 cggcggcctt aataaaggca caaacttcat cggtgaactg cgcctctggg cgtatggttt
    10081 tgttggtggt gtggcccttt tacacattct ctggaaagta ttccaggcat acacggaaat
    10141 ggactcctgg gctggcgttc tcaaatcact gatgtatgcc gcaattgccg gtggttgtgt
    10201 ggtagctgct gaagcctgct accagatttt caaataatta cctaatcacc ggaatacatg
    10261 atgtattccg gttcatcatg aggatcaaaa gttggataaa gaagaaatta aagcagccga
    10321 agagcttaag gcaatctttc ttacctacaa tggcctgaat cgtcctgcaa tgatcaaagg
    10381 tatgcccatc attcctttta ttctgtgtct gctggctctc atggtttctt tttttgttgg
    10441 catactgact attgattttt acgggcttat tattccctcc attattatcg gcgtaatgat
    10501 cgccatcaaa caaatgtgtg ccgatgaccc gaacgccatg tctgccaggg cactaaaatt
    10561 taaaggtctg tgccttaagg gatttcgttc gaataacgtt ctgaaggttg aataatatgt
    10621 cgataaagaa aataaccaat aatttcattc ctccttatca ctcacatatt catgaaaata
    10681 tcatctggct tgaagataaa aaactactgc tcacttttct tattgaaggc attccctatg
    10741 aaagcctgac agatgatatg attctgaaca actttaacgg acaaaaagaa acacttcttg
    10801 gactctgtaa aagtgaaaga gtttttctgt ggaaacatct tgtaaaacgc gaatcaccta
    10861 tggatgccag atataaattt cctggcaatc cttttcttca gcgcttaggt gatttctact
    10921 gtgatacttt taacggagaa agatcattca gaacagatta ttttctcaca ataggcattc
    10981 cttatgatga tatcgatgct ggcgaaaaaa aagctaaaga tatcatccgg cagattgaaa
    11041 caggttttaa agactacaat atttatacgc ttgggatcag tgacggcggt atttccgata
    11101 tttgttcaaa ctatctgaat tatttactca atcatgattt tgagaaaata ccgttatctg
    11161 caacgaagat ttcagagagt attaagaaca gcaaattcta tttcagcttt gatactcttg
    11221 aaataaaaaa tcgcacagga cacgataata aattttgttc caattatatc gtaaaagatg
    11281 taccccgagg ctcgcagatt ggtcagtgga attttctgtt atccctgcca tttgagttta
    11341 tcctgacgca atcctttatt cccgattcgc tatcaaaatc cagcaagaaa attgagcaac
    11401 agcttaacaa actcacttcc gtaaaggacg ctggtaagac gcaacacacc gaactccata
    11461 tggcccagga agctatcatc aaccaggaaa ccgtttttgg ctcctggcac agcgtccttt
    11521 ccgtcttcgg aaacacacct gatgaagcca gtgataacgg ttctgaagtc gcggcggaat
    11581 ttgtcaccag tgggcgggga tttcgttttg ttcgtgcagc gtctgaagca ccttatgcat
    11641 ggttcagtca tctgccagcc tcaaaactgc gcccccttgc cacacaacgc acgctcacta
    11701 atgtctgctg cctgatgccg cttcataatc acagctatgg caaaaaatca ggaaatccaa
    11761 ttggtgatgg ctctgccata atgccgctga aaaccgtcac tgatggtctt tactggttca
    11821 acactcacta cagcgcacca gggcgtaatg taacaggaca gatgatcgcg gggcacggaa
    11881 tgctgctggg tgcgactggt gcggggaaaa ccacttttga aatgatggcc gccgcgttta
    11941 tccagcgttt taacccagat atgttcgtga ttgacttcaa ccgctctacc gaactggcaa
    12001 tcagaatgtt cggtggtcag tatttctctc tgtctgaggg tgtgtattcc ggcctgaacc
    12061 ccttccagat cggagacaat gacgatcctg aactgatggc attcctgaaa gagtgggtca
    12121 aacgttgtgc tgttaataac gatggcagcg actgtagcga cagtgaggct gaagagattg
    12181 accgcgccgt tgaaatggtg atgaaacttg aacgtcgcca gcgacgtttc gctcgtctgc
    12241 aatcccgtat acaaagcccc gaactaaacg tccgtctgtc taaatggtgg gataatggcg
    12301 ctcttgcctg ggctactgac agcgaagaaa ataaattcga tcccaatcaa tacagcaaaa
    12361 tcggttttga cacgactgta atcctggaaa cagttaatca acgagatcat cctgcgtgtg
    12421 aagtaattct ttctgttctg tttttctaca aaaaacgaat gcagaaaggc ggtaagctca
    12481 tgctctccat cgttgaagag ttctggaaac cctgtaacta cccaatgaca caggccttaa
    12541 tcaaggcttc gctcaaggcc ggtcgtatga agggggaaat gatgtggctc accagtcagt
    12601 caccggaaga cgcgattaac tgtcaaattt tcgcggctct cgttcagcag acaccgacaa
    12661 aaatcctgct ccctaaccct gatgccaaat acagcggcta taaagagatt gggctgacag
    12721 aaaaagaatt tcgccagctt aaaaagctga aactcgaaag ccgaacaatg ctgattaagc
    12781 aaagtggctc aagtgtcttt gccaaaatgg acctggtggg atttgacgat tttctgcctg
    12841 ttatttcctg ctcaatgaaa gggattaaat tgtgcgaaaa aatcagggag gaaataggct
    12901 ctgacgatcc tgaactctgg tatcccccgt ttatcgaggg gatttatcag atagatgacg
    12961 ttatacggga agctaacagc gatatccctg aagtctggct gcccttcatg atgaaacggc
    13021 tcaaagacaa attgccggaa aaataactgt ctctgtagcc cgatatatgc cgcacaagcg
    13081 gcatgtaagg cggcactctg tacttaaata tggctttgcg ggttatccgc cgaagccccg
    13141 cgccagcggg tactgtcttc tatattctct ggaactgcgc tcgcgccccc tgctaagcca
    13201 aaaccgcacg aaaccaacag ccacgcggct gtttctggtc gaagactgac aatcttaaag
    13261 cgataggaaa attcaatgaa aaagactctg acagccctgt ttctgggtgc caccctgacc
    13321 ggatacagtt ctgtggcaag ttctgtaggc ggtgtcatcg ttaaagaccc tggcagcata
    13381 gctaaaaccg tcgaagtaat taagaaggct gccgatcaga ttgctgaact gaaaaaacag
    13441 gttgatctgg caaccagcca gcttgaagcc tataagcagg aagtgctgga taccaaaaga
    13501 cgactggaag gtataaccga ttattccgcc atttttggca gtgctgaatc ctacatgaaa
    13561 gacttctggg aagatatgaa gaaggaactt accgatgctg atattcgcag tatggcaact
    13621 aaatacggct ttgatacaaa agagtacgac cggatcaaac gccagtacga atcaaagttt
    13681 gaggaaatca cgaaatacga agcgatgagt aaggacctgg agaaaagcgc agaaaagata
    13741 aaggcaactc aaaaggcttt cagtaaagcc gatacgccac aaaaacgcga agagcttcag
    13801 aataacattc ttctcgaaac tgccgccatg cagttaaaaa ttgcacagaa tgaggcagaa
    13861 ataaatcgta tggcccgtga acgtaaatta cgtgaggaag cggcactgat aaaatataca
    13921 gaagataact tttcctttaa ctgataagaa cgtcacaaat cctcttctaa tatatacaat
    13981 tcccctgtaa tgtttgatat catttttaat tcaacataca ctatcaggag gtagctcatg
    14041 ttcaaaaaac tacttggcgg ttttatcctt gcctttactt ttttattatc cggttgcggt
    14101 ggtgataatg gtgaattagc cggaacatat atagaaaaaa atcctgacaa tagtgatgca
    14161 cctcaaaaat ggattatcaa atacgaaaag gataattact acactcaaat atgggtagaa
    14221 aaagacaaga aaggtaatga gcataacgga acaaccaact cattccttat tagagatgga
    14281 gactggcttg caggaaaaga aaaacatttt attcgagtga ttgataaaaa cacgcttcag
    14341 ttaagagatg atgagaacgc tttgtatatc cgtacaaact gaatgctctc tgattaaccc
    14401 acaacgcctg tccctgtgac gggcgttttc ttttacaggt aataacgatg gcttcaaatg
    14461 actccgcttt tgcaaaagcg aatttcgaaa tcgtccagcc gatgttcaat gaattatctt
    14521 cgattattca gaacagcgta accacaaatt ttcagactat tatgcaactt gtgagtccgt
    14581 tgttcggagc cgccgtcatt ctctacggtg ttttctttgc gtgggaaatc atgtacacac
    14641 gcaaggatga tgtgatgatg gaatccgtaa aacgtatggg cgttctgacg cttgtttttg
    14701 ccatgagtac cgccggaggt atgtacctta gttatattgt tcctttcgta cagggaagcg
    14761 gtcaggaagt ggcctccagg cttattggcc aggacggagc cagtaccgga gccgttatcg
    14821 atggccttct taacagactg atagatttag gccatgaaca atttaaggtt tttcaaaaag
    14881 ctgatggctg gtttgattca atgggggcat ggattgtttt tattgttaaa gccattcttc
    14941 ttgctcttgc tggcggcgga tttgttattt actgcgcggc ctatttatta atggccgaaa
    15001 tgatggtcgg catactgctt tccattggcg gtgtgtttat ctcattttct gcgtggccca
    15061 ccacgcgcaa tatgtttaca gcgtgggtgg gatcgtgttt taactatata tttctcaata
    15121 ttgcctatgc ctgtctgttc aaggttctga ttcagtatat taataattat attaatacaa
    15181 acagcgatga tattactaaa aatatctgga gcgtcctttg tgttgcggtt gtattcctga
    15241 ttggcaaatt cctccttgct caaatatcaa cgctgataag taccttaacc ggcggtgtgg
    15301 gtattaacgg tctgacaagc gctaccggag atgttataaa atctctgcgt ggggctggca
    15361 acagcacacg aaatcagctt gggcttggct ttaataaagc agcacaggat gcacgggcgc
    15421 gtaacagtga atatgctgcc gcagcaaaag attatttatc ttcccgcttt ggtggaaaca
    15481 tcaaggccgg ataaatcccc ctctttactc aatcaatata tcttatttat ccggcttaaa
    15541 aggtatgttt tatgtataaa tatatcattc cggcagttgt ctgcttattt ctttctgcat
    15601 gtaccgcaac aaatccccct gaagcacctg aagctacagg aaaatgggag tcattatata
    15661 ccagcgtcga taacattatg aagggatata aataatgggt ttgaaagaat attttaaaag
    15721 ctccaaagcg gaagcacgta aaacacccgc tcaaaaagaa accgataaaa aaattgaaaa
    15781 aaacgtactg gacgcagcaa aagaatttgc gcgtgatcgt gataacgaac tgaagcgctc
    15841 actgcggatc acgcgttttg cgcttttctt ttcgtccgct tctgcggctg caatggcggt
    15901 tgccctcgca gtcatggccc cgctgaaaac aacagaaccg tttctgttac gggtagataa
    15961 aacaacgggc tacacggatg tgatcacccc gttcagtcag gattccggta catatgacga
    16021 ggcggtaagc cgctatttcc tcacgcgcta tctggagaac cgcgaaggtt acgaatggta
    16081 cacaattcag aacatgctga ataccgttga actgatgtct tcctcccagg tattcgccga
    16141 atacaacaac atgattcacg gcccgttatc tccgctgtat aagctgaaga aaagctacaa
    16201 aattcttgtt cgcgtgcagt ccatcacctt tctggatgaa aacacggtac agggacgttt
    16261 tgttaaggca atcaccgatc ccgatggcaa acccgcacct ggatacacgc caacacaatg
    16321 gattgccacc atcaaatttg tatggctaaa agacaagata tcaactgaag cagagcgcca
    16381 gataaacccg ttcgggctgg atgtgctctc ataccgtgtt gatccagagg tggtgaaatg
    16441 atgatgcgaa aactcctttt atccctggtc atatccggta tcactatgac ctgtcatgct
    16501 gcaaaaacgc cagcgtccgc aggttttgac agacgcgtac agcaaatcgt ctataacccc
    16561 aatgacgtaa ccattgtcaa aacaaaagcg ggagaagtaa cccttatcga gcttgaagag
    16621 ggagaaatca tcaagagcga agatactggt cttgctattg gtgatcctga agcctgggat
    16681 ccggctgtca ggggtaacac tatttttctg cgtcccaaag cagcagaacc tgataccaat
    16741 atcgcaattg tgacaaacaa acggatctac tccatccagt tagtcagtac cgaccagaac
    16801 ccgacctatt tattgcgata catctatccg aaaccgccgg aacccgtgaa acccaatgtc
    16861 atgacgggcc ggagtcgtgt tgtcgcgccc tgtactgaag ggaatattgt aaacggtcgt
    16921 tatgaggtgc gtggtagcag gaaaattaaa cctgaagcca tctgggataa cggtcgtctg
    16981 acctgtttcc gctggactgg cgcagctgac cttccggttg tgttcaggat cttaccggac
    17041 ggtaaggagc aactggttaa ttaccatatg gataaaaaca tcatggttgt gcacgaaatt
    17101 tcgccagctt ttgtactgcg tctcggaggt gaggtgatgg aagtcagaac aaaccgtcat
    17161 ataccgcgaa cctggaacga cagcggaaca ttgactggcg aaaccagagt ggagattaat
    17221 aacgatgaaa aataacaacg atattgtgat tgaaaaactc gatgaaattg acacgcagga
    17281 ggcggatacc gacgctcaaa accggaattt catccactcc ggtaaaaaac agggaaacaa
    17341 aaaggcaaaa gcggtcctta tacttgcagg ttttgctttt gttaccgtag tcattctgac
    17401 atccgtttta tcaaaggtgt tttcatcctc gggagaggaa gaaactaccg aaactgcgac
    17461 cacaaaagca caggcagagg tctatgcaca aaatacccag gcaaaaaatc ttgatgacct
    17521 gaaagaaagc gtcgccagaa gcatagagga agtcaaaaac aacgttaatt cagaagagga
    17581 acaagccagg gaagaagcac caccggaaaa caaggaaaat aaaggtgtca ctctgccagc
    17641 agaaaacgaa gctgataccg gatatacaca aaataatggc gatgccccac ccacaccaga
    17701 acagcgtcgt ctgatgggaa gcctaatggt tgatgacagc cggatcacac agtcacagcc
    17761 tggaagttcc ggtcaaaaaa cggcaggcgc tacgccagcg tcagacaaca caaatgtttc
    17821 tggtgatggt gattttttac agggtgctga ctttgctgat ggctctgtat cccgcataaa
    17881 aaatcgtcgc tttctgcttt cggctggaac aacttttccg tgcgtcctga aaacaatgat
    17941 tgtcaccaca tatcctggtg taacgctctg ccagttaacc aaagatgtga tctcggatga
    18001 cgggaaaacg gtattaatcc gtgctggcgc acaactccag ggagaacaga caaaagccat
    18061 tacacaggga atggcacgcg tgtttgtcaa ctggacaatg gttaaggacc gcaatgtcag
    18121 agtgcggatt aatgctctcg gtgctgacag cctcggcgcg tcggggcttc cggcgtgggt
    18181 ggatacacat ttctgggagc gctttggtgg cgcattaatg ctttctctca ttgaagacac
    18241 tctggctgcc ggaacctcac gtctttcccg cgaaaacaac agcgacttca ctatgaacaa
    18301 taccaccgga acgtccagtg aactggcgaa aaccacgctt gataattcaa tcaacattcc
    18361 gccaacaggt tacgtgaatc agggcgaaat gctgactgtc attgtgccac gcaacattga
    18421 tttttcttct gtatacggag tccgctgatg gaaacgatta acatgtttct tgaaagcacg
    18481 ggcgtacagc cctttcttga ggcggagggg cttacggaaa ttatcgtaaa ccgcccgttt
    18541 gaaattatta ccgaaggcgt taacggctgg gaataccatg aagcccctgg agcctcatac
    18601 agtgacctgc ttgatctctc tattgcaatc aacaaataca acaacagcgc agaccccctt
    18661 gatgctctac accctatcaa gtcgctggtt atgcctggcg gtgaacgtat gcagattatc
    18721 atgcctccgg cctgtgagaa aaaatgtatc tcattcacca tacgtaaacc ttccctgagg
    18781 cgcttcacac tggaggatta cgtcagcagc aaccgattca gcaatgtccg cattgtgaat
    18841 caggcaaacc tgagcgccct gagcgcctcg caacaaacat tacttgacct tctcgataac
    18901 accaggtgcg cccccagaac ctggatcagt tttttagaag cagctgtggc cgccagaatg
    18961 aatattctgg ttgtgggtgg tacgggatca ggaaaaacca ccattgcaaa aaccattgca
    19021 gacctgtttc cccgtgatcg ccgcattatc accattgaag atgtgcacga gatgacgctg
    19081 ccaaaccatc gtaatcacgt ccatctattc tataagcaag gcggcgtaca gccccgatac
    19141 ctgattgaag cggcaatgcg tatgaaaccg gatcatatct ttctggcaga attacgtggc
    19201 gatgaggcat ggagttacct ggaagcactg aatacagggc acgaaggcag cgtgacaacc
    19261 attcacgcca acagtgctct ggacgcaatt tcccgtcttg cgtcgattgt caaacagtcc
    19321 acggtggggc ttaccctgga ccacagcttc atactgaaaa ccatcaaaac ttccatcgat
    19381 attgtggtgt tcttcagaca tacacatatg acggagatct actacaaccc gatggagaaa
    19441 aatcaattac tggccgcatg actttttcgt ctgcgccagc agacagaagg ggcgaacgaa
    19501 aataccaggg aagcacagga gtcacatcgc cccgaagcgc ggcgcagccg ctaccgtaac
    19561 cgaccggaac cacgaccacc ttcccgccct gccaggcccg actgactcac agacaacgat
    19621 gacgctgatt tgcgccgcag aatgccgcag ttagcgcgag cgacagcgag tttacgccgt
    19681 cctggactta cctggacggg gcgctgtact gcgcgtgtga gcgtcagcga gctacgggcg
    19741 caactctccg gtgaaaaacc tcacgacaac ggaaaatctg atgaagaaga agaaacttat
    19801 tctgcctgta cttatagtct ggctgatggc cctgacagcc tccggctggc tggggagttt
    19861 tatactggtg aaaattgcct cgctgccaac tgacaatctg caatggagta cctacctctc
    19921 cacgcttgcc tattacggct ggcaaccttc cctcaaaaaa tatctgcttg ccggacttgc
    19981 ggggactctg cttccgttcc tgattgcact catcctgaca atcgttatgt ttgtcgtcgt
    20041 tcgggagaaa aaaacggtgc acggtaacgc caggctagcc aacgatcggg acctggcaga
    20101 cagcggtttt tttcccgacg aaaaggaact gaaaaatcag aaatacccgt cactgctcat
    20161 aggaaaaatg cctgaacaca aaggacgttt taaatcacgc tatctgcaat acagcggtca
    20221 gcagtttctg atgctgtacg ccccgacacg aagcggtaaa ggtgtatcga tcgttatccc
    20281 taacggcatc tggtataccg aatcgatggt attcctggat atcaaacttg aaaactttct
    20341 tgagacggcg ggataccgga aaaatgtgtt aaatcaggaa gtctatatct tcagtcctga
    20401 cggctatgca cgagaaatcg gcggtgaaac agtgctgaca actcaccgct acaaccccct
    20461 ttactatatt cgccgtgatc cggtatatcg ctttggcgac ctggaaaaaa tcgcggtgat
    20521 cctgtttccg atgaccggag gtgaaaacga cacctgggtg gaatccagta aaaacgtttt
    20581 tatcagtctg gttctgtggt tactcgatac tgaacttgag ggcaaattta aagttaatat
    20641 gaactcggtg atgaaactga gtattccggc tgatggctca agcctggcaa aatggatgct
    20701 gaacgagatc agaagtcggg gtaatgaagt tctcactgaa cgctggaaag cgtaccatgc
    20761 tttaccggaa gaggagaaac agggcaaaac gccctccacc tatccgttaa gcgatgacac
    20821 catccgcctg ttccgccagt tcgcagccaa agaacccgag caacaggaaa gcatcatgct
    20881 cgactttaac cgcaccatgt caatgttcgc caaccctgtc gttgctgcgg ctacggaaga
    20941 taacgatttt gatatgcgtg atgtgcgccg taaaaaaatg acaatttact acggcatttc
    21001 tcctggtgca ttaaagcaat atcagcgtct gactaacctg tttttttcac aactgcttaa
    21061 tgagaacgtg cgtactctgc cagagcatga cgcaacactg aaatatcagt gcacactcat
    21121 gctggacgag tttacgtcaa tgggccgcct cgacgtggtt caggtgtccc tggcattcac
    21181 tgcgggctat aacctgcgtt ttgtgtttat tctgcaaaac agggagcagt tatttgatga
    21241 aaaacatggt tacgggaaaa tgggtggaaa cactattctt aaaaactgtg cggttgaaat
    21301 tttctatcca cctaaaaaag tggatgaaag tgtaaaagat gtatcagaaa caatcgggta
    21361 ttatgatttt gtccgcactc aaataagccg tacaactggt aaaaatccca gtacaacccg
    21421 caataaaatt attgaaaaac gggctgttat gctaccacaa gaaattactg atttacggga
    21481 ccagaaattt tcagttaaag gaaagaaaac aagtttttcc ttaccacaaa tcgtaattag
    21541 tgagttttgc cgcccgttta ttgcacataa aattgtcagt tttgatcaaa acaaggagtc
    21601 tttttttgtt aacgcaagaa aatattcccg aaaaaatatc atagacacac ctgtacttga
    21661 tattccacag aatgaagtgg ttcgtcagtt aatgctccag cgggaaaggg aggaagcatg
    21721 gaaagcgacg caacctgctt ttgataaaga tgattttgat gatgataagg aatattttcc
    21781 tggtgatgat gatggtggtg gcggtggtga ttaaccatga tgccgcttta agcggcatgg
    21841 aaggcggcac tctgtagctc aatatggcta tgcgacttat ccgccgaagc cccgcgccag
    21901 cgggtactgt cttctatatt ctttggaact gcgctcgcgc cccctgctaa gcccaaccgc
    21961 acggaattta cagccacgca attgtttctg gtcgaagacc gacaatgccg aagcgatatt
    22021 ttcaatatcc ctgacaattc acggagataa aaaatgaaat ctcgattgct catatcaatc
    22081 acgctacttt ctgctctcac tttcactcta tctgccagcg ccaaaataaa aggactatcc
    22141 gcagccggag atccctgtga ggtacttgtc tgtatggccg aaaaagtaca gggagcacca
    22201 caaccaggtt gcatttcggt gaatcagaga ttctttaaca tccgcgtcta cacacctttt
    22261 tataatcctg gtgcaaccta cggcgcaaga cagaattttc tttatacctg ttcaggttcg
    22321 actgttaacc aggcaacctt aaatatgatt atgcaacgtt atggcagact tttttctggt
    22381 taacacaggt aatgactcat tcttaccttt caaggaaaat atttaacatc actcagtcaa
    22441 aaaaactgaa ggaaccataa tgagctttat aaaaataaac aggcaacgtg aatttatgga
    22501 gcgaagtaat tcaccacgtc ttgtactacg aaaagaaaag agaagaaacg gtattgtaaa
    22561 ttgttatctt tccaagacgg taagaggcga cgatacattc attaccatag agttaaaccc
    22621 tgaacacaat aaaataagat taaaaacaac caattcacca gaaggccata aaatgagtcg
    22681 tggttgtttt tctcttacta aagttttcga atatattttt catgaaaacc ttcacgaaga
    22741 cagaattatt attgaactgg agaaaaaaga aaatgggtgg tggtatggaa attactaaaa
    22801 acagagaaac ccgatttgta actcttgcat cagactttaa caatattaaa gaaatttatt
    22861 ctgatgacga gttaaaccgt ttgctggaag ctgttaagaa ttgcccgacc ataattgtgt
    22921 tgggttgcgc gaacaagggg aaagaaacgc cacttatggc ttcttgttta aggactacac
    22981 cagagcgtat ttcagttgaa gaattcaatg aaattaaagc tatttcttct aaacagaaat
    23041 gacaagtaaa cgccacagat tccagatttt aatctggaat ttgtggggtt gaacaaccga
    23101 agggcgttag tgcacatttt gggtaatgaa ttatcagctc aggaacaaaa acgcggtttg
    23161 tgggccgatg caaatccggt tccaccgtgg gagtggcgat acaaacataa ctaactgata
    23221 gaggttttta tggctttatc ccccaaagaa aagaaagagt tacaacaacg cttcgaaaag
    23281 caattactac ctgaccttga tctgtcattt gaactaaatg gcgaagtata taaatttaat
    23341 tttgataaaa atgagtcagg tgactatatc gatagtttta cacaagcagt atggggaggt
    23401 tatcaggatg ccgaggaatg ggaaaaatat ggtaaacatc aggagacaga attacctgaa
    23461 tgccctgaac cagtcgaggg ttggtgcgac tgtgataaat gtaagtcgat gatttttcat
    23521 aaagagaaaa atatttatgt ttgcctctcc tgccataaac aaacagagcc atttcttcca
    23581 attgactgga actcaaacga ttacgattgg ctaccttatg aagatgaata ctaatgctga
    23641 atacttcaca gtcataatgg atgaaatggc ggcataaaat taaggaggct taaatgaata
    23701 tccatttatt ttcagaagta ttgttctgcg tatgggttat agctttgatt gtgattttat
    23761 ttatagttgt taaatattac aggcgtgtcc attacagact caattcactg tctgaaacga
    23821 taaagcgcac acagggcggt gttaataagc gaatatctga aaacagagaa ctgcttgaac
    23881 ttataaagaa tcaacatcct gaaattctgg atgaatatcc gtgggtaagt ggttggcttg
    23941 attcacagga aaagtttttg gtcgctcttg ctgataagtc tggtatcgat atcaacaaat
    24001 caggacttat ttgagcacac taaagataaa ggaaattact atgtatatac atattgttcc
    24061 taaactttat catctcatgg ctaataaatg tagccttaaa tctatttcca tacctgagct
    24121 tgattttata attgatagtg aatcattgtc tgtaggcaga ccgtggccta ataaatgttt
    24181 atgggttgga atgcgaaaag gaaggaagtc agttaatggc ctcgttttgc aaacagataa
    24241 aaaattaaga tggtttacta ctatatacac ctgggatatt gaagatatgg ggttaattta
    24301 ccatcaagtc aatacctata tagaagatga tgattttgat atggtttcac aggaaattct
    24361 tttaaatggt tcttttgata agtggagtaa cagggttcat tcagcttatg aaaataagcc
    24421 tccagccaga atccagccca aaatggagtc cttattaaat aaacctggtg aaaacagtca
    24481 tgatgtgtgg gaagagttcg aatggggtga cttccttatg tccagagaag aaagcctttt
    24541 acttcacact attcagtctg aacgattgaa tacagattgt tctttattca aaagacaacc
    24601 atctattgaa agtgcattag ttatataaat cagatactct ttggtggtta ttatgcaata
    24661 tgcattagtt gatgtgctgg aacgaaaatt tttgctggat gctcttgaat ttggtgttct
    24721 gaaggactgg aaagaaaatc cggtaaaaga acttcctgat attgatgaat ctgttcaccc
    24781 cttccatgtc tgctatggtg gatatttatt aaatcctggt gtttcagatt cagatattag
    24841 cagaaaaata aaagaccaga caggattctg gctggcagct attgatgata cccgcatgga
    24901 ttgtcattca atagcttatt atgatattca caccctccct ttgattttgt gtggacatca
    24961 aaagatagtt ccttttgccg cattaataaa agctgatgaa tgcatcattt caaaaattgc
    25021 ttcatattct ggttttgctg taacagcctt tttgagaatt aaagaccagg atatcgcaac
    25081 gaatatactt aaccgtgagg gaatttttgc ctttaatggc tgtgagcgca gattcagaca
    25141 acctgtaagt gaagataact ggcaacaggc agtatcagaa gaacgcgcta ttcgttgtgc
    25201 aaacagatta attcaatgta aaggataaca aaatgagact ttttatcgca gaaaaacccg
    25261 cagtagcaaa tgatattgtt aaggcacttg gtggcaattt tacccgccat gatggctggt
    25321 ttgaaagtga taacgccatt gtgactaact gttttggtca tattatcgaa tcacagccgc
    25381 cggaaaacta taatcctgaa tacaaagcct ggaaggttga aacgcttcct ttacgtcttt
    25441 atcccgtgaa gtatcagcct gttgaaagtg cagcaaaaca ggttaaaacg attctcgagc
    25501 ttatcagacg tggagacgtg actgaaattg ttcacgctgg cgatcctgat gatgagggac
    25561 agctacttgt tgatgaagtc ctggaatatg caggaaacac aaaacccgta aagcgcgttc
    25621 tgattaacga caacacgctt ccggcagtga aaaaggcact ggcaaatctt aaagataatc
    25681 gtgatttcaa agggctttac cttaaggcgc tggcgcgttc agttgctgat gccgtctatg
    25741 gcttctccat gacgcgtgct tacaccattc ctgcaaaagc cagaggatat cagggcgttc
    25801 tgtctgtcgg gcgcgtccag actcccgttc ttggcctgat tgtgaatcgt acccgtgcta
    25861 accagaacca taaatccagt ttttactaca ccatgaccgg agtctttcag cgtggtgctg
    25921 atgttatcag ggcgaactgg aagccagggg aatttgctcc gctgacagac cgtaaattac
    25981 ttgataaagc gtgggcagac ggaacggcag catcccttgc aggaaaaccg gctacagttg
    26041 aagcggcagc aactgatgat aaaaaaacgg ctgcaccgtt gccctttaac ctggtcagac
    26101 tccagcaata catgaacaag aagttcaaaa tgactgcaca aaaaacgctg gatattacgc
    26161 aacaactacg cgaaaaatat aaagcaatta cttataaccg ctctgattgc tcatatcttt
    26221 ctgatgaaca attcagcgaa gcgccgcagg ttatcgatgc cctgaaatca gtctttcctc
    26281 agtcgctgga tattgattct gcacgtaaaa gcaaagcgtt taacagtgca aaggtgactg
    26341 cgcatactgc gataatcccg accgccagtg tgcctgatgt taacgcactc agcaccgacg
    26401 agcgcaatgt ttacctggcg atcgcacaac actatcttgt tcagttcatg cctgaaaaag
    26461 cataccagga agtatcggtt gccattcagt gtggtgatga gtcgttctat gcccgtgcca
    26521 gaaaaacaac tgacagcgga tttgaggcgt ttcttggcgc ggaaaccaca gacgaaggtg
    26581 aatcagaaaa taatgatgat tccgcttttg aacagctttg caaaattcgc actggtgaaa
    26641 cactgacaac aaaagaagtt gttgttaatg agaagaaaac aacaccgccg ccgttattca
    26701 ccgaagcctc cttgcttgct gcgcttgttc gtgtcgcgga ttttgtcact gatccagtga
    26761 ttaaaaaatt actgaaggat aaggataaag acaaaaaaga tgaacatggc ggtattggta
    26821 cgccagctac acgcgctgcc attctggaag cgctgaagaa gagaaattat atcacgctgg
    26881 aaaaagggaa acttattccg acggataccg gatgtgcgct tattgatgcc ctaccaggta
    26941 tagcggttaa tcctgatatg acagcattat ggtctgaaaa acagtctgcc attgaaaatg
    27001 gcgacctgac ggttgaacag tttattaatg atctgtacgg tgaactgaca ggcatgattt
    27061 ctgatgttga cctgggcgaa atgaaaattg aacccacagc gccagccgga caatctccac
    27121 gcctgagttc tccctgccct tcctgtggta aagcgattat tatcagacca aaaggttatt
    27181 tctgtacagg atgtgaattt aaaatctgga aaaatttttc tggtaaggca ttatccgata
    27241 agcaagtgga aacacttgtt acaaaaggta ttaccggaga attaaaagga tttgttagtt
    27301 caaaaacgaa taagaatttc tcggctaagg ttaaacttgt tgataaaacg gccggaaagc
    27361 tgggatttga atttacccac aaaaaatcaa tatagtatct aatgacacgt ctgaagtatc
    27421 cgattaacca ggggtggttt gtcgacttta gattgtgcca tatatggagt tgttagaaga
    27481 aaagggactt cctccggtcg gagccaccaa ctcaaggccg gaaaacaaaa aagcccgatt
    27541 accagtcggg cttttttgtt tctgattaac gggaccgcgt gttatccctg ttcctttccc
    27601 gttcacgctc atttttcaaa gcgttgtcat aactcactat ttcacgcttc acggcctcta
    27661 ccgtttcagg gtttttcatt gctgccttga gatcgtttac atcctcaaaa cgtatgaatt
    27721 tgagcgtatc tttgactgtt tgtttgtcaa tattcatttc cttcgccagt tgcttagaaa
    27781 ttcgctcaat ttctctttcc atcgcttttt cactattgac agtccgcttt ttcttttctg
    27841 tagtattctc gtttttttcc tcgactgatt tagctttttt ctgcccttgt tgttcctcaa
    27901 aggctttaat ctgttcatta atcgtctgag cacgggccga aatactgtta acccctttcg
    27961 aaagcagatc aacctcttct ttcggcgcac cattttctac agcttcattc agtaaacgct
    28021 gggcttcctg gtgctggctg gcaataactt ttgcctcttc ctgtagctgc tgtagcgatg
    28081 cgttattttg ctgatgagga ttttcagtgg ctaacgcgga tttgctttgc tcatctccgg
    28141 tctgtttatc ctgaccagta gtcacgtctt tctctgttac cggcttctgt tgctgcgctt
    28201 cttgtggctg ctgctcgcgt tcttcagaat gagccgaaac gttctgctgc acagactcct
    28261 gtttttccgc ttcctgtacc acggatgttt cagacgtgga tttttgagaa acagtttctg
    28321 acttaacagg ctgttccggc actgcatcac gcgatttatc atcattacgt acagattccg
    28381 ctacttcggg cgttctttcc ttagcctggg tgtctttctc gactgaatta acctgctctg
    28441 ctctcttttc ctgctcctgt tctggtttct gtccggcgac ttgttgctga cgctctgcct
    28501 cgacgctgtt ttcatgtcca tgttcagctt tcagttcctg gagacggacc tgcatcattt
    28561 catgatattt ttcaggtcgc agaataacat tcatcccctt ctcggctgcc gccattgcac
    28621 aggctacctt aaattcttcc gagccattga gtttcagcat cccaccaaat ttctcttttg
    28681 cataaagaag ggctgcttca gctttatcct gggtaatttc accatttaca cgaacatgat
    28741 ttcctttatc agtgaaaaca gtctgtgttg tcttcggatc aaggtaacgc gtttcttttg
    28801 ggctgacctt atgaggaaca agatcggtta gttcacgttt tacataatcc tgaacttgct
    28861 gggattgctg tactcgctta actacgctga aaacttcctt ttcattaccg gaagaaatgc
    28921 tgtttccatc aaccggatta cggattatct tttttaaaga atcacctaaa gccatatcat
    28981 atctcctgtc tggattccaa cggtgatagt atgagtttat ttcgcgaaaa catgcctttc
    29041 gaattgagtt tatttcacgc tctctttcca gctttttaaa tataatgtac cctctagcaa
    29101 cctcccgttc ctgcttgttt cttatcgcca gaagccctct gagttctgcc ctgtaattag
    29161 ctatattatc attatgctta tatttaatct cattatttac ccaaccttta tgttcattta
    29221 aacaaataaa tctttctttt gcctgttgac gcgtaagatt tacacgggta ataacagcat
    29281 caaaaggttt gttatctttc tgatcctgcc agatatcaag taatacctct tttgcctcat
    29341 cccattcaag attcagatgt ttagttaaaa aatcactggc attcatattt cgattgccaa
    29401 ctctgaaacg cggagatcca tcctgtccat aggtgacagt atgctttgat ggatctatgt
    29461 taaagcgtgt ttgcagattg gacaaataac gttcagcatt aatttctgtg cgaatttgtt
    29521 tcaaaaaagg taaatcatca ctgcgctgaa tcgccgcttt aatatttgat tcaagcgctg
    29581 actgaactac tcttcttcct gctctatcct gcaaccgtcg cacatatcga tctggatcga
    29641 ctttttgtcg tccagccgtt gataaataac cgtattcatt ttgctgcaat aacaactgag
    29701 atttattaat gcttttccca cctgatcttc cgtgaagtcc gtaaaccagt tcgcgtgctg
    29761 gcagacgtgg caatccgatt gtcttaccgg atacaaactt tccggtattg tatttaatac
    29821 cggactggtc actattctgt cgtcggtcct gtctttgaag gttgtgtttt tcgtcataac
    29881 gattaattac ctttttcaga tacatttgct tatcttcacc agcaagtgct gcatattctt
    29941 tacgaatttt ttgccctgcc ggatgaatat gttttatctc atgactgatc gaactcttcc
    30001 actctttcag atgactctca atctgcgtct ttgtgggttt taaaagtggt atagttcgat
    30061 cagagatata ctgacgtgaa aataaagggc ttttgagtcg agtaaattta gagtctcctt
    30121 cgagcttcac accaaagtag cgatcagact tccccttatt atactccttt acctcaccaa
    30181 aacgggaaag ataatcagaa aattgtttct ctgttcggat attctctgta tccagcgcat
    30241 caaaaaccgt ggttttaaac tcactgtgcc gctcacggta aaaatcgcct ttagccctgg
    30301 ataatacatt agcgtggtta tggtcactga ctctgacacc atctttagga gaaacaagat
    30361 tgtatttatt attaatgtgc tcctgtatgg catcaagatg tttcaaataa tggttaataa
    30421 ctcccccagg atttagttta tttcctgtca ccagattaac ttcagggata acaatgtgaa
    30481 tgtgaggctt acgctcaacc atttctcctg ttttatgatc aggcatctgt tttatttttg
    30541 gaagatgcgc ttccgcataa aaattatatt catcttcatg ataagcgttc atcatgagag
    30601 acttgtattc ctggactaca tcatgcaact ttgattcaga aacatcatct tcaaaaaagg
    30661 ataacgtaat atgcatatag cgctcttgac cattatcagg tatcgcgtca ataactgatt
    30721 cagtcaatgc cagatcgcca tccagaacta atcgatggtc aatttcatct cgggtatatt
    30781 cccttccttg cttatgacct ttttcaaggt attcgcatac ccccttattg ccaccgctga
    30841 ttctgactaa catttattaa tccctgcaac aaacgcatca cgaatattaa caagaacatt
    30901 aagatagcgt aaatatgtac tttctgaaat aaaaccattg cgatattctg tgttaatttt
    30961 tttggctatc tgattaatat tattgcttga cttattaaaa agtctgagta attctctgta
    31021 ttcaactgga cgtttttgtt tcaaagtcgg aatatcatcc agacgattca gtataaccat
    31081 ccgaaaaaac tcactacgac tgacttcaag agatgtaaaa tactcatgaa atgactgata
    31141 ttcctcatca gtcaatcgca gtgatatcac atgttttctc tccacctttg atgtggaagc
    31201 tacagccttt tttcctttgt cttgtttttt catggttcgt agaacctcca tatagcgacc
    31261 tggttgtaga gatatactgg ggtattaggg ggagatcccc ctaagcacgt cgattatacc
    31321 ggaatttatt ccgtgtatac atcgctgtcg tgcctgtctt ttagaaagtt caactcgacg
    31381 aatcatagcc ccacaaaaca acccacataa tcacccacac acaaacacaa gcaacccaca
    31441 aaacaaccta cacttaaacc cacaaaaaag cctacaacca tgttgacaac ccacaaaaca
    31501 acccacataa tcacccacac aaacaaaatg caaattgatt tgaataaaat attgtgccaa
    31561 tgtaggaggt aataagagag gatatataac aaatgaaaaa gatcgatagt ttattatctc
    31621 caatatgcga ggcgataaaa gaaacaaatt caaaatcaaa acaggctgtc ttcaatatgt
    31681 atttatcgaa catacttgaa gcaaaaagct attatacatg ggagcaaata acagattaca
    31741 taaataacaa aaccaagaca gaactagccc aaaagactta tataaacatg accgagaggg
    31801 ccaaaaaaag gcaaaaatgc actgggcaaa aagacctaaa aaaaagcacc aatatgattg
//
LOCUS       DBBBSB010000035        31276 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.035, whole genome shotgun sequence.
ACCESSION   DBBBSB010000035 DBBBSB010000000
VERSION     DBBBSB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31276
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.035"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(435..1364)
                     /gene="virB"
                     /locus_tag="ACIUS0_001636"
     CDS             complement(435..1364)
                     /gene="virB"
                     /locus_tag="ACIUS0_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HGB0219423.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(1581..1817)
                     /locus_tag="ACIUS0_001637"
     CDS             complement(1581..1817)
                     /locus_tag="ACIUS0_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HGB0219424.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(1821..3722)
                     /gene="ipaA"
                     /locus_tag="ACIUS0_001638"
     CDS             complement(1821..3722)
                     /gene="ipaA"
                     /locus_tag="ACIUS0_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HGB0219425.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(3731..4729)
                     /gene="ipaD"
                     /locus_tag="ACIUS0_001639"
     CDS             complement(3731..4729)
                     /gene="ipaD"
                     /locus_tag="ACIUS0_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HGB0219426.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(4780..5928)
                     /gene="ipaC"
                     /locus_tag="ACIUS0_001640"
     CDS             complement(4780..5928)
                     /gene="ipaC"
                     /locus_tag="ACIUS0_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HGB0219427.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(5891..7633)
                     /gene="ipaB"
                     /locus_tag="ACIUS0_001641"
     CDS             complement(5891..7633)
                     /gene="ipaB"
                     /locus_tag="ACIUS0_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HGB0219428.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(7639..8106)
                     /gene="ipgC"
                     /locus_tag="ACIUS0_001642"
     CDS             complement(7639..8106)
                     /gene="ipgC"
                     /locus_tag="ACIUS0_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HGB0219429.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(8163..8789)
                     /gene="ipgB1"
                     /locus_tag="ACIUS0_001643"
     CDS             complement(8163..8789)
                     /gene="ipgB1"
                     /locus_tag="ACIUS0_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HGB0219430.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(8805..9194)
                     /gene="ipgA"
                     /locus_tag="ACIUS0_001644"
     CDS             complement(8805..9194)
                     /gene="ipgA"
                     /locus_tag="ACIUS0_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HGB0219431.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(9207..10691)
                     /gene="icsB"
                     /locus_tag="ACIUS0_001645"
     CDS             complement(9207..10691)
                     /gene="icsB"
                     /locus_tag="ACIUS0_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HGB0219432.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            11005..12621
                     /locus_tag="ACIUS0_001646"
     CDS             11005..12621
                     /locus_tag="ACIUS0_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HGB0219433.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            12631..12993
                     /gene="ipgE"
                     /locus_tag="ACIUS0_001647"
     CDS             12631..12993
                     /gene="ipgE"
                     /locus_tag="ACIUS0_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HGB0219434.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            12993..13451
                     /gene="ipgF"
                     /locus_tag="ACIUS0_001648"
     CDS             12993..13451
                     /gene="ipgF"
                     /locus_tag="ACIUS0_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HGB0219435.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            13460..14575
                     /gene="mxiG"
                     /locus_tag="ACIUS0_001649"
     CDS             13460..14575
                     /gene="mxiG"
                     /locus_tag="ACIUS0_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HGB0219436.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            14583..14834
                     /gene="mxiH"
                     /locus_tag="ACIUS0_001650"
     CDS             14583..14834
                     /gene="mxiH"
                     /locus_tag="ACIUS0_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HGB0219437.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            14847..15140
                     /gene="mxiI"
                     /locus_tag="ACIUS0_001651"
     CDS             14847..15140
                     /gene="mxiI"
                     /locus_tag="ACIUS0_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HGB0219438.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            15146..15871
                     /gene="mxiJ"
                     /locus_tag="ACIUS0_001652"
     CDS             15146..15871
                     /gene="mxiJ"
                     /locus_tag="ACIUS0_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HGB0219439.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            15868..16395
                     /locus_tag="ACIUS0_001653"
     CDS             15868..16395
                     /locus_tag="ACIUS0_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGB0219440.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            16337..17032
                     /gene="mxiN"
                     /locus_tag="ACIUS0_001654"
     CDS             16337..17032
                     /gene="mxiN"
                     /locus_tag="ACIUS0_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HGB0219441.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            17025..17432
                     /gene="mxiM"
                     /locus_tag="ACIUS0_001655"
     CDS             17025..17432
                     /gene="mxiM"
                     /locus_tag="ACIUS0_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HGB0219442.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            17416..17844
                     /gene="mxiM"
                     /locus_tag="ACIUS0_001656"
     CDS             17416..17844
                     /gene="mxiM"
                     /locus_tag="ACIUS0_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HGB0219443.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            17974..18606
                     /gene="mxiE"
                     /locus_tag="ACIUS0_001657"
     CDS             17974..18606
                     /gene="mxiE"
                     /locus_tag="ACIUS0_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HGB0219444.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            18593..20293
                     /gene="mxiD"
                     /locus_tag="ACIUS0_001658"
     CDS             18593..20293
                     /gene="mxiD"
                     /locus_tag="ACIUS0_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HGB0219445.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            20312..21379
                     /gene="mxiC"
                     /locus_tag="ACIUS0_001659"
     CDS             20312..21379
                     /gene="mxiC"
                     /locus_tag="ACIUS0_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HGB0219446.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            21392..23452
                     /gene="mxiA"
                     /locus_tag="ACIUS0_001660"
                     /gene_synonym="virH"
     CDS             21392..23452
                     /gene="mxiA"
                     /locus_tag="ACIUS0_001660"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HGB0219447.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            23465..23866
                     /gene="spa15"
                     /locus_tag="ACIUS0_001661"
                     /gene_synonym="spaK"
     CDS             23465..23866
                     /gene="spa15"
                     /locus_tag="ACIUS0_001661"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HGB0219448.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            23870..25162
                     /gene="spaL"
                     /locus_tag="ACIUS0_001662"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             23870..25162
                     /gene="spaL"
                     /locus_tag="ACIUS0_001662"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HGB0219449.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            25243..25593
                     /gene="spa13"
                     /locus_tag="ACIUS0_001663"
                     /gene_synonym="spaM"
     CDS             25243..25593
                     /gene="spa13"
                     /locus_tag="ACIUS0_001663"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HGB0219450.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            25580..26458
                     /gene="spa32"
                     /locus_tag="ACIUS0_001664"
     CDS             25580..26458
                     /gene="spa32"
                     /locus_tag="ACIUS0_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HGB0219451.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            26452..27333
                     /gene="spa33"
                     /locus_tag="ACIUS0_001665"
                     /gene_synonym="spaO"
     CDS             26452..27333
                     /gene="spa33"
                     /locus_tag="ACIUS0_001665"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HGB0219452.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            27338..27988
                     /gene="spa24"
                     /locus_tag="ACIUS0_001666"
                     /gene_synonym="spaP"
     CDS             27338..27988
                     /gene="spa24"
                     /locus_tag="ACIUS0_001666"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HGB0219453.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            28003..28263
                     /gene="spaQ"
                     /locus_tag="ACIUS0_001667"
     CDS             28003..28263
                     /gene="spaQ"
                     /locus_tag="ACIUS0_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HGB0219454.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            28263..29033
                     /gene="spa29"
                     /locus_tag="ACIUS0_001668"
                     /gene_synonym="spaR"
     CDS             28263..29033
                     /gene="spa29"
                     /locus_tag="ACIUS0_001668"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HGB0219455.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            29040..30068
                     /gene="spa40"
                     /locus_tag="ACIUS0_001669"
                     /gene_synonym="spaS"
     CDS             29040..30068
                     /gene="spa40"
                     /locus_tag="ACIUS0_001669"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HGB0219456.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            30090..30323
                     /locus_tag="ACIUS0_001670"
     CDS             30090..30323
                     /locus_tag="ACIUS0_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HGB0219457.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(30930..31276)
                     /locus_tag="ACIUS0_001671"
                     /pseudo
     CDS             complement(30930..31276)
                     /locus_tag="ACIUS0_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         9756 a         4576 c         6165 g        10779 t
ORIGIN      
        1 gaaatctgct aatatattct taaagctttt ttgatactgt gaacgaagtc tgttaatggt
       61 ggattttttg tttatcacca tgtaaaaaaa attgagtagt aaaagcgttg actgctaatc
      121 ctcgctgttt ttattgaaac gattaaaaat acgctgaata ttttcggcca gtcactcata
      181 ttcacataaa attttgacca tcatgccgca tcccttagtt gcacaattca ttttcaatga
      241 tatgaatatt actaatttca ccagataatt ttctcgtggt cagcagggta tgagttttca
      301 ccgtaatttt aaggaaacac agtgcgtggg gattgaataa cagatagccg acggaattac
      361 cccaccggct gaaagtcaag ttcaacgaag atttttatta tctgaattgg gcagtttaca
      421 tcagtgttcg atgtttatga agacgataga tggcgagaaa ttatatcccg aatagcttca
      481 tctatttctc tctgaactgt tttgtttatt ttagataatg taaatattag agttctgttt
      541 gttttattct cttttctttt tatgtaagtc cgtttgtctt tagaaataaa taacgagtca
      601 acttttagcg aagggttttg ctttctgttt ttgcttttct ttatgatgtt taaaatttct
      661 tttttatata tgtccggggg caaatttgta tcaaggtctt ttatttcttc ctttaatatt
      721 ggtagtgtag aactaagaga ttcattagcc ttttcaagtc ctttataata attgaataat
      781 atcttgtagt cattaaagtt aagttctgac gcgattggaa atagagatat tatttcctgt
      841 ggaacgcttg ctgcctgaaa ggcacgagtg actttcgcgc gagacagatt ctcttttttg
      901 gctatgtctt tataggacat ccctgatact ttcagaaaat taagaccaat accaagttct
      961 cggatgctat gctcttttgc tgtttgtata tcgtttgcta gttttctggc atcaagagta
     1021 gatatatatt cttttgaata tagaacttca agatctgctc ctgcatatat tgcagatgct
     1081 cttctacgag tgccatccag aatttcaatt ctaccatcaa tctcccttcc tattacaggg
     1141 aagaattgtt gtagctttat agtttttatg atatcggcta atgattcttc tgttagggat
     1201 tcttgatcgc gtacattcgt tttttggttg acgaaggtta aatcttggat ccttttatga
     1261 ggaatcttgg ctttgataaa ggaaacttta ttaccagaat ggagtgtaaa ctctttcttt
     1321 tgtccttcct ttatacttaa caagtcgttg cacaaatcca ccatatcaca ccctgtttat
     1381 tcatattgat ttatattgac aaaagttaaa tgcagtgtga gcaagattgt atgtaactgt
     1441 tgatgatagt caacggtagt cttaaaaatt actattttaa tagctggatt gatgcaattt
     1501 atctttattg gataaaagaa taatattctt gtggagctct gatgtgagag ctccatagat
     1561 gaaaaaatgc ttatcaatta ctatattgat tttgtactat ttttgactac agaaataaga
     1621 tctccaatag taataatgtg ttctaatgta gattcatcta tacgtagatt gaactcaaaa
     1681 ctaatcatat ccagcatttc aatcagagaa agagaatcgg cagaaagatc ttcaataaat
     1741 ttagtttctt cagatagttt gctgtacgct ataccatagc aaaaggccac tattgataat
     1801 attttttctt ttatcatatt ttaatcctta ttgatattct ttaatacttt tgatagggaa
     1861 gtggttacat cttttgctgc tttatatata ttattgtttt cagctgtaat atcattgtta
     1921 ttttttaaat cagatatatc atctgtaagt aattctgcag aggtatcgtc aatttttgac
     1981 aataccttgc tgagggcgct agatacttct tttgcctttt catagatcgc atgattttta
     2041 tcaattgtat ctgcgttggt ttctgttacg tagtttctta tatttgcatc tgtaactttt
     2101 aatttgttgt ttattctatg ttctgttgtg gattttgtaa tgtcctttga tttttcctgt
     2161 aactctcgtt cttgtgttcc tgatttagtt ccaatgagag atataagatt acttagggag
     2221 tttgttactt tttttgaagc ttcaaatatc gtttcccgtg ttaaatatgt gtctccattt
     2281 cttgataaaa aatcagtctg aagtactttt gaatcagtgt ttgagttgaa tttattgatt
     2341 tgcgaagcta ctctatgaga aagagaagcg ctacttttag ttgtatctga tgagttatca
     2401 ttactgcttg tcactccttc cgggtggaga gggggggtgg ttgaagagtt ctgtatgttc
     2461 agaggcctgt gtccccgaga aagagagtta tttgcagatc tttcttgact actcgtagtt
     2521 aacaggtctg aacctattcc atccgaatta tctggagtat ggttgaaagc cttttctaaa
     2581 gctgaattat ttgcaaatgt ctggacagta tctgatatag agtgtggtag aacggaagtt
     2641 actttttgaa ctagtgctct ttgtggattt agtaatgagt ttccgttacg ggataataga
     2701 tcgtttgtct gagaagtagg agactgtgca tcattttctg ggttctcgtg atagctattg
     2761 tcatatactc tgttgtcaaa aactctatta tcgtaagttc tattatcatt atttatatga
     2821 tagtgaatta caggttggga tgttttctca ttggctccgg gggttggtcc gccatctggc
     2881 atatcttcgg gaggtgtagg gaggccatct tggataggat gcgaatcttt tatatcttct
     2941 aaaatatcat taaggacttt tctcaacagc tcaggaaaca cggctactgt atttaagtct
     3001 cttgacagtt tacctatacc aaatccttta ctatcaatag tatttttacg ttgattgcgc
     3061 aaactatcaa aggcaagttt tgcctgtgcg tcaactaaat catctagttt gagagatgaa
     3121 acagtttcat tacttaactt taattcagca agaatattat tcttgataaa tgatgatact
     3181 tcagtattga ggagtttttg tcccaatgat aaagcttcac cagatgtgtg ttgttgtgct
     3241 aatagtgcaa gtctgtgctc aatgttttgt cgtaattgat ggagtttctt attctttgcc
     3301 gaccatggtc caaaggcaga atcttgggat aatttatctt ttataattag ttccgaatac
     3361 tgtgcgacaa ttttttgaag tcccttttcg ttttcaaaat taagaccatt ttgatgtact
     3421 aaggagtaca aaacatttga aaattcgaga atctcgcttt tggcatacgc attcattgag
     3481 ttggaataaa tgcttcttaa cacatcttct ttccctgagg aagaaaataa aaaatcaaga
     3541 catttctgat taaaagacgt tgcaaagttt tctttttcag acactgatgc tggtgttttt
     3601 agagaagttt gcgaactatg gatatcggat attttgcttt ttaactcgct gtattctgtt
     3661 gatgatggtg aagttgcctt atataagaat gttggcgctt gagtattatt tacattatgc
     3721 atggcgcacc tcagaaatgg agaaaaagtt tatctgtatc tgtacatgag cttattgtac
     3781 tactcaaaac ctttactaaa ttatcaaaaa tactattggc attactgtat ttttgaacta
     3841 aagtttgaag attatttttc attgtttcat cttcggcaga gaatccggca ttccatgcct
     3901 gatattttgc attatctagc acaacctcgc catttccacc tagattatct aagcttttta
     3961 acatattgtc tattggggtc atgtttatac tgacaacata tcccccgttt ttttgagata
     4021 ccttgccgat tgttccacct aattctgtaa gccatttatt tgcttgttcc tgactaacag
     4081 tattatttgc tggatatagc ggtttatctt tatatttttc cttgagttct tccaatgcct
     4141 ttttaagcga gttgacttgt aatttcacgg agtttccgtc gttacctccg ggagagatcc
     4201 agccggcaag actggaaaga acagcgctaa aatcttgata catttgagta tatgaactaa
     4261 cggcatgttc atatactttc agatactgtt cattaatatc attgatggag tttgcaattt
     4321 tagcccacag ttctctatga gatatcattt gatctccatc aagctcggct tctttcggag
     4381 ctgaatgtaa taattctctt gcgtctttat taattggata ttcgtgcctg gaaagaatat
     4441 ctaatagttg agcggattta tttacatcca tgctaatctg agatgaatgt aatgctattt
     4501 cttctagcga tgttttagtc aacgtttttt gtgaaagctc tttctttaat gcctgatttg
     4561 ttgttctgat attatgaagg gtgtcgttga gcatagtaag ggaacttaca ggatgagaac
     4621 tggtcgttgt ttttatatca gaattaactg tttcggttga tgaaccgttg gtattgtttg
     4681 gactgaatga tgaggtggaa atactattag tcagagttgt tatattcatg gttatttcct
     4741 tacaggaaga gccatatatt attatggctc ttggatatat taagctcgaa tgttaccagc
     4801 aatctgactg gctgtcgaat tctttgattg gttgatgctg tcaattatat tcaataattt
     4861 ttgtattaat tgatttgtcg cttgggatgc ttctttagat acttgggatg cttcctctgc
     4921 ttgtttactg ctggcctgac tgattagttg ttcttcttct tcaagagcag atgcataacg
     4981 ccctcctgat gtggatatat taccagcaac ggctgatgtt tccattgttg cacggccaat
     5041 gttttgtttt tgctgcgcag aaagggtatt gagctcgtaa gttcttctct gggtgtcaat
     5101 tttatcctgc aaagaaatat cgggagaact aagagaagtt ttatgttcag ttgacaggga
     5161 tatattatct ggcgccagtt tatttttacc taaaaatttt gtgctagagt tagtttgtgg
     5221 tgaggtgata tttgtatcta tttgtttatt taaccctaat ttagaacctg caagctcttt
     5281 ttcaagagat tgagcagtgg caaggttctt tcttaaggct cctttttggt cgctaatccc
     5341 tgaatgcgtt tttttggcac cgatacccgt tatacctact tgtgtgactg ctccagtaat
     5401 gcttgatgat agggctgcca ggccttgccg aacaatgttc tctgcagctg attttgtagc
     5461 atcgaacgca atcaatgaca attgagagcc caatttagtt tctgcagtgc ggagagcaga
     5521 aagtagaacg gctacactaa taattaaaga aacagcatta gaagaaagac tggaaatatc
     5581 cagagtgttc tctggagtaa gggtgtgact catctctgaa agatctttca tctttttgtc
     5641 agtaaggcga gcaatgattt ctgaatcatg ctgaacctgc tctgataact ttaaaagttg
     5701 atcatcattt aatgtggttg ttaatacggg atttttaccg acattaagtg gaatatgcgc
     5761 tgcaatctgc tgataatttt gtgatttttg tgtttcggaa gaactttgag tttgttttgt
     5821 ggatatatct gtatataaaa tctgggttgg ttttgtgttt tgaatttcca taacattctc
     5881 cttatttgta tcaagcagta gtttgttgca aaattgcttt tgcaacatca gttctattag
     5941 cctgagagtt ggacattgag gctaatagat ctgcaattac ttcctgcaat tggccgaatt
     6001 tttctattgc ttcactgata tattttgaca gttgttcaac ttgatatttc gataatgtca
     6061 ggtctgctag atttgtcgac gcgctgttct ggaaaacagc agaagcgaca cttcctcccg
     6121 cttgtgtggc agagttaaca ctttctccta ataaaactgc ttggtttaaa tgggtggaaa
     6181 taatttgttt ggatattact tcatcacccg ctgcaccaag aaatttattt aatctggcaa
     6241 cagcattagt gattaaatcg tccagttgag aagaaaaatt cttgagaaac tttggtataa
     6301 ggtctgtgag ggttttacct attattttgc caatattttc tgcaagtttt gctgctgcct
     6361 gtttaccaac agtggctacg agaacgactg ctgcaactag gacaagagcg cctgcgattg
     6421 cccccagaat agagccaatc attttggctt ttttcgagtc gacgcccaag ccttcgagca
     6481 tttttgtaaa tgcatctgaa aggagtttga ttaagggttc aatgactgct ttcatgatcg
     6541 gattcagggc ttgttccatg aaggaattgc cggtcgctgc ttgtactata gcatccgtaa
     6601 ccataagagc taaaccaaca gctgccagtg ctagagaggc tcctccagaa aaagctgctg
     6661 caacaacact aacgatagtt aaaagtgccc caagtatttt cccaacacaa cccattactc
     6721 tgttgagttc ttctgcttta cgtacttcag cagcatactc atcagatttt ctctccattt
     6781 cagtttttct tgattcttgg agagactgga atagagccag atcatttttt aaagattctt
     6841 cattattttt tccaactagt tgaataaagg ttgccatcaa ttgagtaaca ctggcaagtc
     6901 cggttaatga tttctgctgg gttgatagct gttcagcaga tgctgtgttt gaaaatgcag
     6961 aaaaagagtc tatttctttt tcgagttgca ttgatttatc tgtaagtttg ctatgaattg
     7021 acagggtttt ctgctcaatc aatgtcctgt ctttaactgc tgcgtctttt ttgatagtga
     7081 gttgtatttc ttcccggctt aattttttct tttctggtga ctctgggtcg agttcggata
     7141 atcttgtttg aatttggtta attttatttt ctaggtcttt tattttagaa tctgcgtttt
     7201 ttagtttatt aatttgtttt tcatagtctc tggttagtcc ttcagtttca gatagaagag
     7261 tgttaatttt atcggagaat tctaggtttt tttgctgtct tgcctgttgc tgggacttcc
     7321 aagcagtaat tttatttgtt aatgcagtta aagatttttc accgagtatt tgaataaggt
     7381 ttccaattaa aagcgttagt tgggaacttg catttaatga ctttggtgct ttaagttcag
     7441 ggattaatat atttgaagtt gagtgtgcat tagttgtatt aatattttgg ttagcagtaa
     7501 gatcagcaat tgtaagagaa aataatttgt tagctgcatc atttgcagct tggatagtat
     7561 tgtctccaag ctcagtggaa gccaatattt tggcaagagg aaaaccagtg gttgtggtgc
     7621 ttacattatg cataataatt actccttgat atcctgaatt gcgtccaagt atgattgtgc
     7681 ttttattttt aatttttcat cattgctgtg ttgaattacg agttcgaagc actctttagc
     7741 ttttaagggg gctttcaacc gaagctggca ttgtccagta tggaatactg gtgtatagtc
     7801 attttttcct aatgcaaaag cgacagcata aaggtctgct gcttgttgga actgttcttt
     7861 tatctgataa atagctgcga gtcccataat gtagtctaca ttgtaaaagt cgtatataca
     7921 taaaaacctg aagaaaactt cagcttcctc tattcttcct ttgttgtaaa agtcataagc
     7981 atatgaataa atgtcatcca tcatatcatc aggaattgca ttaatatctt tcagtgtagc
     8041 gccagagtta attgcatcaa ttactgcagt agagatgctt tcattttcgg tgatatttaa
     8101 agacataagg tctccttttt gtctgcttta ctttaataag tataagattt aatataaaag
     8161 atttaatttg tattgctttg acggtataca gcctttagaa cctctgcgct tatttctatt
     8221 tgtaaaactt ttgttgattc attaaatatt ttatcgtaga cggaactgga tactagattt
     8281 gtaagatcct tcatttgtgt gttaaacaaa tccatgtcac tgctatagcg ttttaaaatc
     8341 tttgctctga ggccagatga ggcaatctcg tttagtgctg tatgactgat agatgtttgt
     8401 gctgattttt catcaagttt tatatccggg aatttttttt caatagctgc aaatatgttt
     8461 tttttgttat ctgaggtcaa aacaactcca ttgtgtatgg ccatttcatc tatacttctg
     8521 tttatcattg aagagacata agtggttcgc tcttggctca tccaacaaaa tagttgattc
     8581 cccgaattac tatcttttat tttgtctatg acgctgtccc tgattttatt tatattcacc
     8641 gggcatccaa aattcttctg aggaaagcgt tgttttaagt taaaaacaga taatattttc
     8701 tttacaagct tattgcgact taacgaatca aatgatgggc gaggaatcgc gaattcaacc
     8761 tgtggaagta ttttgtttag aatttgcatg atacccccta tatgttagtt cacttctgaa
     8821 gtgatgtttg caatggttag tttaatacaa aataaaaatt catacaaaga ttcaatgagt
     8881 tcatcaggtg aaagatattt tttgtagtgt ttcttggtca aaacacattg atgagtatca
     8941 gggagaagac agatagaaga accataatct ctactcaaag agtagttaag gatgagtatg
     9001 tgttctggat taataatatt ttctgtaaaa agttcatgtt cattaagaag tccggtcaaa
     9061 aagatgtatt catcattatc tgttatacaa acaggtattt gttcttcgag caatataaag
     9121 gcttgatttt tatcgttaaa atcaagcttt gcacctgtta tttcataaag tttatcatat
     9181 agtttgcgac acatttctat ggccttctat atattagaat gagagttatt caataaaagc
     9241 ttattgatct gtaataagtc gtgaatgtaa agtttggttc caagatctgg cgatttaaga
     9301 gaattgtaat aatcaataat ttttattgat ttttcgatga tttcttcttt tttatctgtt
     9361 gcttcggttt ttaattcgtt taatacacga gtcatattgc tgggtgctga ttttactaag
     9421 tatgagtggc aagcgttgat gaccgcatca aagtcttctg aagatacatt ttcagtagga
     9481 tgtttgtaaa gtgaaattga taaaggcttg aatgtttttg gtgtgtggat aaggttaatt
     9541 ttattgaaac ttttaagata gctgcgtcgt aattgagtca atctggaaag atcaaaatca
     9601 gattcaatat acttcattat ttcgttataa gactgattga gactttcaat gcgttgccta
     9661 actttatttg catacgcgta tacatcgtta ggtgtagcaa ccaatttaac gtcagggagt
     9721 ggaaagtata tttcagaatt tcctgcttta agaacattaa gtgccatacc agcacagttt
     9781 tcagatttac tgattaattt ataatttgcc aattgtctga aagcatctgc tttctttttg
     9841 catataaatt ttgacatgtt tgtttcatca aggccaaaaa ggttatggct gattttacca
     9901 tcctttgttt tattatcacc tgataatgga atgtatactt tatcagcact gaccccccaa
     9961 taaccatagg ggtctttttt ttgattagct ctgggaaaaa atattttttt gttgttattt
    10021 tgatcttgtt ttgaatttaa taagtttcta acctcttggc ctgcatcaag tctttcggct
    10081 gtttggtctg aaatcatatt tagtttgtct tgcttatagg tatcgacact gtaacctgag
    10141 cgtttttcaa aaaggtagtt tatttctgta tcttttgtaa cattcccgcc gggataccag
    10201 ctgagatagg tatgagttgg ttcctgtagt aaattgtttt ttatactgat actagtatgc
    10261 cctaaatggg ttatgcctct atttccccag caacgaaaaa ctaaatgtct ttcaagattt
    10321 ttggctgtac cgatgccatg aaaacgctct gcggtattca acattaattt gatgtctcta
    10381 cctgtgaaag agtgttgaat tgagttttta gctgcttcat aggcggcaaa cgcactttct
    10441 gcagattttt cgctagctaa tgtattgagg aaaagcaata aggatttttg attctctagt
    10501 cgataatttt ttagttgttc atttaatatc tgtgaattta tctttgcatt aatagtgctt
    10561 aatgttctga aaaagagatc ctttaggttg aatttctgtg ctatcaatat aggtccatgc
    10621 tctgtatctt caacgcgtat aggccctttt gtattgcttg cgtcaatgaa attgctaatt
    10681 ttgaggatca tactttatta actctccatt acttggtgat tgtggggatg taaaatgtat
    10741 ttggtatata atatattcaa ttaataaatt tagaaacttg agcctgttaa cataatcaaa
    10801 ttttcttttg ctgtacataa tatgtacctc gtgagcatat gtagtgctcg tttcatcatg
    10861 aaatcccaca agataaagtg cctgatgtat caggctcgga gtgttataga aaaagagaga
    10921 accctgttga ataagtatct tttttgttgt aaaacgaggt acaaaactgc aatcaatgcc
    10981 ccatttatga ctgaggataa ttaaatgcac ataactaatt tgggattgca tcaggtttca
    11041 tttcaaagcg gagattccta taaaggcgca gaagaaaccg gaaagcacaa aggtgtaagc
    11101 gtgatttcat atcaaagagt taaaaatgga gaaagaaata aggggatcga agcgcttaat
    11161 cgattatatt tacagaacca aacaagctta acaggaaaaa gccttttgtt tgcgcgtgac
    11221 agagctgaag ttttttgcga ggcaataaag ttggcaggtg gtgatacgtc aaaaattaaa
    11281 gccatgatgg aacgattaga tacctataaa cttggtgaag ttaataaaag acatattaat
    11341 gagcttaata aagtaataag tgaagagata agagcacagc taggcattaa aaataaaaag
    11401 gaattgcaaa cgaaaataaa acaaatattt actgattatt taaataataa gaattgggga
    11461 ccggtcgaca agaatattag tcatcatggg aaaaattatg gttttcaatt aactcctgcc
    11521 tctcatatga agatagggaa taaaaatata tttgtcaaag agtataatgg aaaaggaatt
    11581 tgttgtgctt ctacgagaga gagtgatcat atcgcgaata tgtggctttc aaaagtggtg
    11641 gatgatgagg ggaaggaaat tttttcgggt atcagacatg gagttatatc agcctatgga
    11701 ttgaaaaaaa actcatctga aagagctgtt gctgctcgta ataaagctga ggagttagta
    11761 agcgcagcat tatatagcag accagagtta ttatcacagg ctttgtctgg taaaacagta
    11821 gatttaaaga ttgtttcgac ttctcttctg acgccgacca gtttaaccgg gggagaggag
    11881 agtatgttaa aagatcaggt gaacgcatta aaagggttaa actctaaaag ggggaagcca
    11941 acgaaattat taattcggaa tagtgatggt cttctgaaag aagtaagcgt taatctaaaa
    12001 gtggtgacat ttaactttgg tgtaaatgaa ttagcgctaa aaatgggctt aggctggagg
    12061 aatgttgaca agcttaatga tgaatcaata tgttctttgt tgggggataa tttcctcaaa
    12121 aatggtgtga ttggaggttg ggctgcagaa gcaatagaaa aaaatccacc atgtaagaat
    12181 gatgtgatat atctggctaa ccagataaaa gagattgtaa ccaaaaagtt acaaaaaaat
    12241 gataatggag agccgtataa attgtcacaa agaatgactc tattggctta tactataggt
    12301 gctgtacctt gctggaattg taagagtggg aaggacagaa caggtatgca ggatgctgaa
    12361 attaaaagag aaataataag aaaacatgaa acaggtcagt tttctcaatt aaatagtaaa
    12421 ttatcctcag aagagaaaag attattttct actattctaa tgaatagtgg taatatggaa
    12481 atccaagaga tgaatactgg tgtgcccgga aataaagtca tgaaaaaatt gccgctatct
    12541 tcgttagagc tatcttattc tgaaagaata ggggacccaa aaatatggaa tatggtgaaa
    12601 gggtattcgt catttgtata agaggaatat atggaagatt tagcagatgt tatttgccga
    12661 gccttgggta tccctttaat tgatatcgat gatcaagcaa tcatgcttga tgatgatgtg
    12721 ctaatatata ttgaaaaaga aggagattca ataaatttat tatgcccttt ttgtgcattg
    12781 cctgaaaata taaatgatct tatatatgcg ttgagcctaa attactcaga aaagatatgc
    12841 ttagctactg atgatgaagg cggaaatcta attgcgcgat tagatcttac cgggataaat
    12901 gaattcgagg atgtatatgt taatacagaa tattatattt cgcgtgtgcg ttggctgaag
    12961 gatgaatttg cgcgaagaat gaaggggtat taatgtcccg tttcgtattt attttgctgt
    13021 gttttatccc atatttaggg agagctgatt gttgggataa ggctggtgaa aggtacaata
    13081 ttccatcaag tttgttaaaa gcgattgcgg aaaaagaatc cggatttaat aaatctgcag
    13141 tcaatgttaa taacaatgga agtaaagatt atggtataat gcaaataaat gactttcatt
    13201 ctaaaagact tagagaaatg ggatattctg aggaaatgct aattagtcat ccatgtcttt
    13261 ctgtacatta tgcagcaaag ttattaaatg agtttatgat gatgtatgga agagggtggg
    13321 aggctgttgg agcctataat gcaggaacgt ctccgaaaaa gaaaaaagaa agattaaagt
    13381 acgcagagga tatctataga cgctatttgc ggatagcagc agagagcaag cagaataatc
    13441 gaaggatata agaggattta tgtctgaggc aaagaactca aatcttgcac cattcaggtt
    13501 attagtgaaa ctcacaaacg gtgtcgggga tgagtttccg ttatattatg ggaataactt
    13561 aattgttctc ggacggacga tagaaacact tgagtttgga aacgacaatt tcccagaaaa
    13621 tattattcct gtaacagata gtaaatcgga tggtataatt tatttgacca taagcaaaga
    13681 taatatctgt caatttagtg atgaaaaagg tgagcaaatt gatatcaatt cacaatttaa
    13741 ttcatttgaa tatgacggga tttcttttca ccttaaaaat atgagggagg ataaatcgag
    13801 aggccatata ctaaacggaa tgtataagaa ccactcggtg ttttttttct ttgctgttat
    13861 tgttgtgtta attataattt tttccctctc tttaaagaaa gatgaagtaa aagaaattgc
    13921 tgaaattata gatgataaga gatatggaat agtcaacact ggacagtgca attatattct
    13981 tgccgaaaca caaaatgatg cggtatgggc gtctgtagcc ttaaataaaa ccggatttac
    14041 caaatgcaga tatattctgg tgtcaaacaa agaaataaat agaattcaac aatatatcaa
    14101 tcaacgattc ccatttatca atttgtatgt gttgaattta gtgagtgata aagcggaatt
    14161 actggtgttt ttaagtaaag aaagaaatag ttcaaaagat accgaacttg ataaattgaa
    14221 aaatgctctg attgttgaat ttccgtatat caaaaatatt aaattcaact atcttagcga
    14281 tcataatgca aggggggatg ctaaaggtat tttcacaaag gtaaatgtcc agtataaaga
    14341 gatttgtgaa aataataaag ttacatattc tgtcagagaa gaactgacag atgaaaagct
    14401 tgagctcatt aatagactga tttctgaaca taaaaatata tatggagatc agtatattga
    14461 gttttctgta cttttgatag atgatgattt taaaggtaaa tcatatctta acagcaaaga
    14521 cagttatgta atgttgaatg ataaacactg gtttttttta gataaaaata agtgaggata
    14581 aaatgagtgt tacagtaccg aatgatgatt ggacattgag ttcattatct gaaacttttg
    14641 atgatggaac tcaaacatta caaggtgaac taacattggc actagataaa ttagctaaaa
    14701 atccttcgaa tccacagttg ctggctgaat accaaagtaa attatctgaa tatacattat
    14761 ataggaacgc gcaatccaat acagtgaaag tgattaagga tgttgatgct gcaattattc
    14821 aaaacttcag ataataggga gcattcatga attacattta tccagtcaat caggttgata
    14881 ttatcaaagc cagtgatttt caatctcaag agatatcaag tctggaagac gtcgtgtcgg
    14941 ctaaatatag tgatattaag atggatacag atattcaagt atcacaaata atggagatgg
    15001 taagcaatcc agaatcatta aacccagaat ctttggccaa gttacagacg acgctctcaa
    15061 attattcaat aggagtatca ttagctggca cgttagcaag aaaaacagtt tcggctgttg
    15121 aaactttatt aaagtcttaa tttatatgat taggtataaa ggttttattt tattcttgtt
    15181 gctgatgttg attggatgtg agcaacgtga agagttaatt tctaatttat ctcaaagaca
    15241 ggcaaatgaa ataatatctg tgctagaacg ccataatatt actgctagaa aagttgatgg
    15301 aggtaaacag gggatctcgg tacaagtcga aaaggggaca tttgcatcgg cagttgattt
    15361 gatgcgcatg tacgatttgc caaatccgga gagagttgat atctcacaaa tgtttcctac
    15421 agattcatta gtgtcttctc caagagctga aaaggcccgt ttatatagtg ctattgagca
    15481 acggctggaa cagtctttag tttctattgg tggtgttatt tcggcaaaaa tacatgttag
    15541 ctatgatctt gaagaaaaaa atatatcttc aaaaccgatg catatatcag taatcgctat
    15601 atatgactca ccgaaagagt ctgaactatt agttagtaat attaagcgat ttttgaaaaa
    15661 caccttttct gatgttaagt atgaaaatat atctgtcata ttaactccga aagaagaata
    15721 tgtttataca aatgtacaac ctgttaagga agttaaatcg gaatttttaa caaatgaagt
    15781 aatatattta tttctcggga tggctgtact agttgtcatt cttttggtat gggcattcaa
    15841 aacagggtgg ttcaagagaa acaaaatatg ataagaatgg atggaattta taaaaaatat
    15901 ctttcaataa tttttgatcc agcgttctat ataaatagaa atcggttgaa tttgccttct
    15961 gaactgttag aaaatggcgt aatcagaagt gagattaata atctcataat taataaatat
    16021 gatctaaatt gcgatattga acctttaagc ggggtaaccg ctatgtttgt tgccaactgg
    16081 aatttacttc cagctgttgc ttattttatc ggtagtcaag agtctcggtt aattaatcat
    16141 agtgagatgg ttattagtta ttatgggggc aaaatcagta agcaagggga ggccgctatt
    16201 cgaagtggtt tttggcatct tattgcgtgg aaggaaaata tatctgttgg tatatatgaa
    16261 agaataaact tattatttaa tccaattgct ttggagggaa attatacgcc tgtagaaaga
    16321 aatttgtccc gattaaatga aggtatgcaa tatgcaaaaa ggcactttac cggtatccag
    16381 acatcatgcc tatgatggtg ttgttataaa gagaattgaa aaggaattat gtaaaaccat
    16441 aaaagataga gacactgaga gtaagaagaa agcaatttgt gttataaagg aggctacgaa
    16501 aaaagctgaa tcactgcgta tagatgctgt atgtgatggt tatcaaattg gtatacagac
    16561 tgcttttgag catattattg actatatttg tgaatggaag ttaaagcaaa atgaaaatag
    16621 aagaaatatt gaggactata taactagttt gctttcagaa aatttacatg atgagaggat
    16681 cattagcact ttattggagc agtggcttag ttcgttgcgt aatacagtaa cagaattaaa
    16741 agtggttttg cccaagtgta atttagcact gaggaaaaag ttagaacttg atctccataa
    16801 atatcgctca gatgtcaaga ttattttaaa atattctgaa gggaataatt atatattctg
    16861 tagtggtaat caagtagtgg agtttagtcc tcaggatgta atatcaggtg ttaaaataga
    16921 acttgcggaa aaattaacaa aaaatgataa aaaatatttc aaagagttag cacataagaa
    16981 actgagacaa attgcagaag acttgttaaa agagaatcct gttaatgatt aatcaaataa
    17041 atgcaagcaa tgctctgcag caaagattaa atagtgaaga agttgttaat ttaaatgaga
    17101 gattatcatc ttcgcaatca tttgatgagg atataatcta tgaaataatg caatatttct
    17161 cacagagtga attaaattcc atagataatg atgaactgca caataagatt gaacaattat
    17221 tcaattcacg ttttccttat cttacagctg cacaaaaaag ttcacttctt aataaactca
    17281 tagatgcaaa tcaatatgtc gatttgcatg agggatttta tgcatcattg tccatatata
    17341 acaatattga tttctatatt aagactacaa ccttcgattc attaattagt gtctttgaag
    17401 cagggagaga ggcagatgat tcgacatggt agtaataagt tgaaaatatt tattttaagt
    17461 atattgctat taacactgag tgggtgtgct ttaaagtcat catctaattc tgaaaaagaa
    17521 tggcatattg ttcctgtaag taaggattat ttttctattc caaatgattt attatggtcg
    17581 tttaatacaa ccaataaaag tataaatgtt tactctaaat gtattagtgg taaggcggtt
    17641 tatagtttta atgcaggtaa attcatgggc aactttaatg ttaaggaagt agatgggtgc
    17701 ttcatggatg cacaaaagat agctatagat aaactatttt ctatgctgaa agacggggtt
    17761 gttttaaaag gtaataagat aaatgatacc atccttatag agaaggatgg ggaagttaaa
    17821 ttaaaattaa ttcgagggat ataattgtat tgtgagtaaa tataaaggtc taaatacaag
    17881 taatatgttt tacatttact ctagtggaca tgaaccagtt aacgttgagc ttgtaaaaga
    17941 taaagaacgt aacataattg agctggctcc agcatggaag ggtttttttt tgtccgaaat
    18001 caaaatataa aattcagtga taatgtgaac tatcattatc gctttaacat taatagttgt
    18061 gcaaaatttc ttgctttttg ggactatttc agtggtgctc tagtagagca tagccatgct
    18121 gaaaaatgta ttcatttcta tcatgagaat gatcttcggg atagttgtaa taccgaatcg
    18181 atgcttgata agttaatgtt aagattcatc ttttcatctg accagaatgt aagtaatgca
    18241 ctggctatga tacgaatgac tgagtcatat catttggttc tttatcttct gagaactatt
    18301 gagaaagaaa aggaggtaag aataaagagt ctgacagagc attatggtgt gtcggaggct
    18361 tattttagat ctttatgtag aaaagcttta ggggcaaaag tcaaagaaca actgaacact
    18421 tggcggttag taaatggttt attagatgta tttttgcaca atcaaacaat cacttcagct
    18481 gccatgaata atgggtatgc atcaacatcc catttttcaa atgagataaa aacacgacta
    18541 ggcttttctg caagagaatt atcgaatatt acttttttag tgaaaaaaat aaatgaaaaa
    18601 atttaatatt aaatctttga ctctcttgat tgtattgtta ccattgatag ttaatgctaa
    18661 taatattgac tctcatttat tagaacaaaa tgacatagcg aaatatgttg ctcaaagtga
    18721 tacggtagga tctttttttg aacgtttttc tgcgttatta aattatccta ttgttgttag
    18781 caaacaagct gcaaaaaaga gaatatctgg ggagtttgat ttatctaatc cagaagaaat
    18841 gcttgaaaaa ctgacattac ttgtgggact catatggtat aaagatggaa atgcattata
    18901 catatatgac tctggagaat taataagcaa agttattttg ctggagaata tatcacttaa
    18961 ttatcttatt cagtatctga aggatgctaa cctttatgat cacagatatc ctataagagg
    19021 aaacattagt gataaaacat tctatatatc tggtcctccg gcattggttg aactggtagc
    19081 taatacagca actttgcttg ataagcaggt ttcttctatt gggacagata aagttaattt
    19141 tggggttata aaattaaaaa atacatttgt tagtgacaga acttataata tgagagggga
    19201 ggatattgtc atcccagggg tggctactgt tgtagaacgt ttattaaata atggtaaggc
    19261 tctgagtaac agacaagccc aaaatgatcc gatgccaccc tttaatatta cacagaaagt
    19321 atctgaggat agtaatgact tttcatttag ttcagttacc aatagttcta tattagagga
    19381 tgtatcactt attgcatatc cagaaaccaa ttctatatta gtcaaaggta atgatcagca
    19441 aatacagatt attcgcgata ttatcactca gttagatata gccaaacgac atatagaatt
    19501 atcattatgg ataatcgata ttgataaatc tgaattaaat aaccttggtg ttaattggca
    19561 aggaacggct tcatttggtg attcgtttgg tgcgtcattt aatatgagct catcagcatc
    19621 gataagcact ttggacggca ataagtttat tgcttcagtt atggcattaa atcagaaaaa
    19681 gaaagcaaat gtagtttcaa ggccggtgat tttgacacaa gagaatattc cggctatatt
    19741 tgataataac aggacttttt atgtgtcatt agttggtgag cgcaattctt cgttggagca
    19801 tgttacgtat ggaacattga taaatgttat acctcgcttt tcatctcggg gacaaattga
    19861 gatgtcttta acgatagagg atggaacagg aaatagtcag tcaaattaca attataataa
    19921 tgaaaataca tctgtccttc cagaagttgg aaggaccaaa atcagtacaa tagcgagagt
    19981 tccacaaggc aaaagtttgt tgataggtgg ttatacgcat gaaacgaact ctaacgaaat
    20041 aatatctatt ccttttctct cctctattcc tgtaatcggt aatgtgttca agtataaaac
    20101 aagtaatatc agtaatattg taagggtttt tctgattcaa ccccgtgaga taaaggagtc
    20161 aagttattat aatactgcag aatataaaag cctcatatct gaaagagaaa tacaaaagac
    20221 tacacaaata atcccatctg aaactacttt gctggaagac gaaaaatcat tggtttcata
    20281 cttaaattac taactataaa gtaggtgatg tatgcttgat gttaaaaata caggagtttt
    20341 tagctctgca ttcattgata agttgaatgc aatgacaaat tcagatgatg gagatgagac
    20401 tgctgatgca gagcttgatt ctggcttggc taatagcaag tatattgact catctgatga
    20461 gatggcttcc gctctttcgt catttataaa cagaagagac cttgagaaac tgaaaggaac
    20521 aaatagtgat agtcaggaac gtattttaga tggggaagaa gatgaaatta atcacaagat
    20581 ttttgattta aagagaacgt taaaagataa ccttcctcta gatcgggatt ttatagacag
    20641 actaaagaga tattttaaag atccaagtga tcaagtctta gcattaaggg aacttttgaa
    20701 tgaaaaagat cttactgctg aacaagtcga attattaact aaaattatta atgagataat
    20761 atcaggtagt gaaaaaagtg ttaatgctgg aataaattca gctatacagg ctaaattatt
    20821 tggcaacaaa atgaaacttg aaccacagct tttgcgtgca tgttatcgtg gttttatcat
    20881 ggggaacata tcaacaacag atcagtatat agaatggctt ggtaattttg gttttaatca
    20941 cagacataca attgtgaatt ttgtagagca gtcactgatt gtagacatgg attctgagaa
    21001 accgagctgt aatgcttatg agtttggttt tgtgttatct aaattaattg caattaagat
    21061 gattagaact tcagacgtaa tttttatgaa gaaactggaa tcctcaagct tgctaaaaga
    21121 tggcagttta agtgcagagc agctattgct aactttatta tatatttttc aatatccaag
    21181 tgaaagtgag caaattctta cttctgttat agaagtatca cgagccagtc atgaggattc
    21241 tgtagtgtat caaacatatc tatcttctgt taatgaaagt cctcatgata tatttaaaag
    21301 tgaaagtgaa agagaaattg cgatcaatat tctacgagag cttgtcacaa gtgcatacaa
    21361 gaaagagctt tctagataac aggagataaa agtgatccag tcttttctta agcaagtaag
    21421 tactaagcct gaattaatta tattagtgct tatggttatg atcattgcta tgttgatcat
    21481 accattacct acataccttg ttgatttttt aatagggctt aatattgtac ttgctattct
    21541 cgtttttatg ggatcatttt atattgaaag aatattgagt ttttctacat tcccttcggt
    21601 tttgttgata acgacattat ttcgtctcgc tttatcaata agtactagtc gactaatttt
    21661 agttgatgcg gatgcgggta aaattattac tacatttggc cagtttgtca ttggtgatag
    21721 tctggctgta ggttttgtta ttttttcaat agtaactgtt gtgcagttca ttgttataac
    21781 aaaaggatct gaaagggttg cggaggtagc tgctcgcttt tctcttgacg gtatgccggg
    21841 gaaacaaatg agcattgatg cggatttaaa ggctggaatt attgatgccg caggagctaa
    21901 agaaagacgc agtattctgg aacgtgagag tcaattgtat ggttcatttg atggagcaat
    21961 gaagttcatt aaaggtgatg caattgctgg catcattatt atttttgtca atttaatagg
    22021 aggtatttct gttggtatga gtcaacatgg aatgtcccta tctggtgcat tatctactta
    22081 taccatacta actattggtg acggattggt ctctcagatt cctgctctgt taatttctat
    22141 aagtgcagga tttatcgtta caagagttaa tggtgatagc gataatatgg gacgtaacat
    22201 aatgtcccag atatttggga atccttttgt tctcattgtt acatcagctc ttgccttggc
    22261 gatagggatg ttgccaggtt ttccgttttt tgtttttttc ctgatagcag ttactttgac
    22321 ggctttattc tattataaaa aggtcgtaga aaaagaaaaa agtctgtctg agtctgattc
    22381 tagtggctat actggtacat ttgatattga taatagccat gactcatctt tggcaatgat
    22441 agaaaatctg gatgctatta gttcagaaac cgttccttta attttattat ttgccgaaaa
    22501 taagataaat gcaaatgata tggaaggtct tattgaaagg ataagaagtc agttctttat
    22561 tgattatggt gtcaggcttc caactatttt atatagaaca agcaatgagc ttaaggtcga
    22621 tgatattgtt ttgttaataa atgaggtgcg tgccgatagt tttaatatat attttgacaa
    22681 agtgtgtatt acagatgaaa atggagatat agatgctcta ggtattcctg ttgtttcaac
    22741 ttcatataat gaacgtgtta tttcttgggt agacgtttca tatacagaaa atctaactaa
    22801 tattgatgct aaaattaaaa gtgctcaaga tgaattttat caccagttgt cacaagcttt
    22861 attaaacaac ataaatgaga tttttggtat acaagaaaca aaaaatatgt tagatcagtt
    22921 tgaaaatcgg tatcctgatc tattaaagga agtcttccga catgtgacta tacagagaat
    22981 ttctgaggta ttacaaagat tgcttggaga aaatatttct gttcgcaatt taaaacttat
    23041 tatggagtct ttggcgcttt gggctcccag agaaaaagat gtcataacat tagttgaaca
    23101 tgtccgtgca tcactttcta ggtatatttg tagtaaaata gctgtttctg gtgagattaa
    23161 agttgtgatg ctttccggat atattgagga tgcaataaga aaagggataa ggcaaacctc
    23221 tggtggctct ttcttgaata tggatataga ggtttcggat gaggtaatgg aaactttagc
    23281 acatgctttg agagaattga gaaatgcaaa aaaaaatttc gttcttttgg tatcagtaga
    23341 tatacgtagg tttgttaaaa gacttataga taacagattt aagagtatac tcgttatatc
    23401 gtatgctgag attgatgaag catataccat taatgtatta aagactattt agtgaggttt
    23461 aaatatgagt aacattaatt tagttcaatt agttagagat agtcttttca cgattggttg
    23521 tcctccatca attattacag atttagacag ccattcagcg attaccattt ctttggatag
    23581 tatgcctgct attaatatag ctcttgtcaa tgagcaggtt atgttatggg ctaattttga
    23641 tgcaccaagt gatgtaaaac ttcagtcatc tgcttataac atattaaatt taatgttaat
    23701 gaactttagt tatagcatta atgagctggt agagcttcat cgttctgatg agtacttaca
    23761 gttaagagtt gttattaaag atgactacgt tcacgatggg attgtttttg cagagattct
    23821 gcatgagttt tatcagagga tggaaatctt aaatggggtc ttataatcaa tgagctatac
    23881 aaaattgctc actcaattat cttttcctaa tagaatctcg gggccaatct tggaaacaag
    23941 tcttagcgat gtttcgattg gtgagatttg taacattcag gctggaattg aaagtaatga
    24001 aattgttgca agagctcagg ttgtaggatt tcatgatgaa aaaacaatat taagcttgat
    24061 tggaaattct cgtggacttt cacggcaaac gttgattaag cccactgccc agtttcttca
    24121 tacgcaagtc ggccgtggat tattgggagc agtagtcaat cctttagggg aggttactga
    24181 taagtttgct gttacagata acagtgaaat tctttatcga cctgtagata atgctcctcc
    24241 gctatatagt gaaagggctg caattgagaa gcctttttta acaggtatta aggttattga
    24301 ttctttactc acgtgtggtg aaggacagcg aatggggatt tttgcgtcag ctggttgtgg
    24361 caaaactttt ctcatgaata tgctcattga acatagtggt gctgatatat atgttattgg
    24421 gttaattggt gagcgaggtc gagaggttac tgaaacggtt gattatttga aaaactctga
    24481 gaaaaaaagc aggtgtgttt tagtatatgc aacttcggat tactcttcgg ttgatcgttg
    24541 taatgctgca tatatagcca ctgctatagc cgaatttttt aggactgaag gacataaagt
    24601 agcgcttttt attgattcat taacaaggta tgccagagca ttacgtgatg tggccttagc
    24661 cgctggagaa tcacctgcca gaagaggcta tccggtttcg gtttttgata gcttacccag
    24721 acttcttgaa aggccaggaa agttaaaggc aggtggctct attactgcat tttacactgt
    24781 tcttttggag gatgatgatt ttgctgatcc attagctgaa gaggtaagat ccattttaga
    24841 tggacatata tatttgagca gaaatctagc ccaaaaagga caatttcctg caattgattc
    24901 cttaaaaagt ataagcaggg tatttacaca ggttgttgat gaaaaacatc gtattatggc
    24961 cgctgcattt cgggagctac tttctgaaat agaagagcta aggacaatta ttgactttgg
    25021 tgaatacaaa ccgggggaga atgcctctca ggataaaatc tacaacaaaa tatctgttgt
    25081 tgagagtttt ctgaagcaag attatcgact cggttttact tatgagcaga caatggagct
    25141 tattggtgaa acaattagat aaggttttaa aaatcaaaga taaatatcaa cggtctgtta
    25201 aattaattga agcacacata ttaacactaa aaaaaaaact ctttgtatcg agatgtggag
    25261 gcattagata aaaggattat atacttcctg caactggaga atgacttaga acctgtaggc
    25321 gctcagagtg ttagtcagct tttcaataca agacgtaaga tcgctatagt aaaaaagcat
    25381 attatccaat atcagtctga acgaattctt ctaaagggaa gaattgaaga aattcaaaaa
    25441 gatattgatg aggcaaatgc ttctaaaaga aaactgcttc ataaagagag taaaatatgt
    25501 aaacggattg gcttgattaa aagaaataac tttgccaaac agttgatact tgatgagtta
    25561 tcacaagagg atatgaagta tggcattaga taatataaac ctaaatttta gtagtgacaa
    25621 acagatagaa aaatgtgaga aactatcttc aatagataat attgatagtc tcgttttgaa
    25681 gaagaagagg aaggttgaaa ttcctgagta ctctttaatt gcatctaact attttactat
    25741 tgataagcac tttgaacata agcatgataa aggagaaatt tatagtggca ttaaaaatgc
    25801 gttcgaactt agaaacgaac gagcgacata ttctgatatt ccagaatcaa tggccattaa
    25861 agaaaatatt ttgataccag atcaagatat caaagcaagg gaaaaaataa atatcggcga
    25921 tatgaggggg atcttttcat ataataagag tggaaatgca gacaagaact tcgaaagaag
    25981 tcatacttct tctgtaaacc ctgataatct gctagaatct gataatagaa atggtcaaat
    26041 tggtttaaaa aatcatagct tgtctattga taagaatatt gctgacatca tttctttact
    26101 aaatggaagt gttgctaaat catttgagct gcctgtaatg aataaaaata ctgcagacat
    26161 aaccccatcc atgtcattgc aagaaaaatc aatagttgaa aatgataaaa atgtttttca
    26221 aaaaaatagt gaaatgactt accactttaa acagtggggg gctggacatt ctgttagtat
    26281 ttcagtggag tctggttctt ttgttctaaa accgtcagat caatttgtag gaaataaact
    26341 tgacttaatt ttgaaacaag atgctgaggg taattacaga tttgatagca gtcaacataa
    26401 taaggggaat aaaaataata gtacaggata taatgaacag agtgaagaag aatgctaaga
    26461 attaaacatt ttgacgctaa cgaaaaacta cagattttat atgcaaagca actctgcgag
    26521 cgtttttcga ttcagacatt caaaaataaa tttacaggca gtgaaagttt agtcactctt
    26581 acttccgtgt gtggggattg ggtaattcgt attgatacat tatctttttt gaaaaaaaaa
    26641 tacgaggtat tttcaggatt ttctacacaa gaatctttac tgcatttatc aaaatgtgtc
    26701 tttatagagt cgtcatctgt attttcgatt ccagaactgt ctgataagat tactttccgg
    26761 atcacgaatg aaatccagta tgcaactact gggagtcatt tatgctgttt ttcctcttct
    26821 ttaggtatta tttattttga caagatgccg gtattacgta atcaagtttc tcttgactca
    26881 ttgcatcatc ttttagagtt ttgcttaggt tcatctaatg taaggctggc tactttaaaa
    26941 agaattcgca ctggtgatat aatcatagtt cagaaacttt ataatttatt attgtgtaat
    27001 caagttatta ttggggatta tattgtgaat gataataatg aggcaaaaat taatctgtca
    27061 gaaagtaatg gtgagtcaga acacacagaa gtttctttgg cattattcaa ttatgatgat
    27121 atcaatgtaa aagtggactt tattctttta gaaaaaaata tgacaatcaa tgaactaaaa
    27181 atgtatgtag aaaacgaatt attcaagttt cccgatgaca tagttaaaca tgtaaatatt
    27241 aaagtaaatg gttctttggt tgggcatggg gaacttgttt ctattgagga tggttatggt
    27301 atcgagatta gttcttggat ggtaaaggag taatctcatg ctgagtgaca tgtccctcat
    27361 cgctacactt tcttttttta cgctattacc atttttagtt gctgctggta cctgttatat
    27421 aaagttttct attgtttttg taatggtacg aaatgcactt ggtttgcagc aggttccatc
    27481 aaatatgact ctcaatggta ttgcattgat tatggctttg ttcgtgatga aacccattat
    27541 tgaagctggt tatgaaaatt atctaaatgg ccctcaaaaa tttgatacaa tatctgatat
    27601 tgtccgtttt tctgattctg gcttgatgga atataagcaa tacttgaaaa agcatacaga
    27661 tcttgagtta gcacgttttt tccaaaggtc tgaagaagaa aatgctgatt taaagagtgc
    27721 agagaataat gattattcat tgttttccct cttgcctgca tatgcgctaa gtgaaatcaa
    27781 agatgcgttc aaaataggtt tttatttata tttgccattt gttgttgttg atcttgttat
    27841 ttcaagcatt ttacttgcac ttggtatgat gatgatgagt ccaatcacaa tatcagtacc
    27901 aataaaactt gtattatttg ttgcgcttga tgggtggggt atcctgtcta aggccttgat
    27961 tgaacaatat atcaatattc ctgcttagat gatggaggta atgtgtctga tatagtttat
    28021 atgggtaata aggctcttta tttaatcctt atcttttcct tatggccagt aggtatagct
    28081 acggttattg gattatctat tggtttgtta cagacagtga ctcaactcca agagcagaca
    28141 cttccttttg gtataaagct tataggtgtc tcaatatctt tgctacttct ttctggatgg
    28201 tatggtgagg ttttattgtc tttttgtcat gaaataatgt ttttaattaa gagtggggtt
    28261 tgatggacat ttcaagctgg ttcgaaagta ttcatgtgtt tttaatactc ctgaacggcg
    28321 ttttttttag attggctcca ttatttttct ttcttccatt tttaaataac ggtataattt
    28381 ctccatctat tagaatacct gtgatttttc ttgttgcatc aggattaatt acttctggta
    28441 aggtagacat aggttcttct gtttttgaac atgtttattt ccttatgttc aaggaaataa
    28501 ttgttggcct ccttctctct ttttgcttgt ctcttccctt ttggatattt catgctgttg
    28561 gtagcattat tgacaaccag cgtggggcaa cgcttagtag ttcaattgat cctgccaatg
    28621 gtgttgatac gtctgagttg gcaaaatttt tcaatctttt ttctgcagtt gtatttctat
    28681 acagtggtgg tatggtcttt attttagaat ccatacaatt gtcttataat atatgcccgt
    28741 tattttctca atgttctttc cgtgtctcaa atatcttaac atttctgact ttattggcaa
    28801 gtcaggctgt tattttagcc agtcctgtta tgatagtatt gttactatca gaagtattac
    28861 ttggtgtatt atcgagattt gctccgcaga tgaatgcttt ttccgtatca ttaactatta
    28921 aaagtttact tgcaatattt attattttca tctgttcttc tactatttac ttttctaaag
    28981 ttcaattttt cctcggtgaa cataaatttt tcacaaatct atttgttaga taaaatatta
    29041 tggcaaataa aacagaaaag ccgacaccta aaaaactaaa ggatgccgca aaaaaaggac
    29101 agtcatttaa atttaaggat ttaacgactg ttgttattat tctggtaggg acatttacta
    29161 taatatcatt cttttcctta agtgatgtaa tgctcttata cagatatgta ataattaatg
    29221 acttcgaaat taatgagggt aaatactttt ttgcagtggt tattgtcttt tttaagataa
    29281 ttggcttccc actttttttc tgtgttcttt cggctgtgtt gccaacattg gttcaaacaa
    29341 agtttgttct tgcgactaaa gctatcaaga ttgatttttc agtattaaac cctgttaaag
    29401 ggttaaaaaa aatatttagt ataaagacaa taaaagaatt tttcaaaagc attctgcttc
    29461 ttattattct agcattaaca acctatttct tttggattaa tgaccgaaaa ataatttttt
    29521 ctcaggtgtt ttctagtgtt gatggcttat atcttatttg ggggaggctg tttaaggata
    29581 taatattatt tttcttggca ttttctattc ttgttattat tcttgacttt gtgattgagt
    29641 tcattttata catgaaagat atgatgatgg ataaacagga gataaaaaga gaatatatag
    29701 agcaagaggg acactttgag acaaagtcga gaaggcgtga gttgcatatc gagattcttt
    29761 cagagcagac taaatctgat atacgtaatt caaaattagt ggtaatgaac ccgactcata
    29821 ttgcaattgg tatttatttt aatccagaaa tagcgcctgc accttttatt tctctcattg
    29881 aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa tgaagttggt ataccgactg
    29941 tgcgtgatgt gaaattagct agaaaactat ataaaacaca tacaaaatat agttttgttg
    30001 attttgaaca cttggatgaa gtcctacgtc ttattgtttg gcttgagcag gttgaaaaca
    30061 ctcattaata agtaaaggag atgtgttata tgggagttaa tttctgtaat aaaataggta
    30121 ttgatcagtc tgaatttgaa atagaatctt ccatcattaa ctccattgct aatgaggtat
    30181 tgaacccaat atctttcctt agcaataagg atataataaa tgttttactc agaaagattt
    30241 catctgaatg tgacctggtt agaaaagaca tttatcgttg tgctctggag ttggtcgttg
    30301 aaaaaacacc tgatgattta taatcattca taataactct tttgctgata taaatatttt
    30361 tgttgaagat atttatgtag tcacaggatc aattgtagaa aattgttatg tttttgttag
    30421 gaactgcact aaataatata tgataagaca gcaaaaacga ctgaccataa tccttctttt
    30481 attaggagtt gataagagag actattcatc ctgtaatgtt aaaactctgt tatatagtat
    30541 tcgcgattat gcaaagtctg taaatgatca tgagatacta accgagagta atcgtttatt
    30601 atctcattgc atttcagata gtaacggagc tttctttaaa agtagtaaat acgttcccct
    30661 taagtactta cgtaaaagga gaatagcacg aaagatacca aatgattagc actttgctag
    30721 taagcttgct ggtaaactat attgaccgtt gggtttgttt tgagactgta atttaaattg
    30781 tgtaattgcc tgtttttgat atgtttactc caacaacgga gacaggtaaa ttatggacga
    30841 aaagaagctt aaagcccttg cggctgaact ggctaaaggc ctcaaaactg aggccgacct
    30901 caatcagttt tcccgtatgc tgacgaaata tcccggctct taaaacgata gtggctcagg
    30961 ctttcatttt tcagcgttcc ccagaagatt tccatcggag cgttgtcgta acagttacct
    31021 ttacgcgaca ttgatgtttt cagaccagac tgctcctgta tgacccggta atcgtatgcg
    31081 cagtactgtg aacctcgagc ggagtggtgg attagcccgg caggtgggtg ctggctcctg
    31141 agcgccataa acagggcttt acccgtcaac tctttcgtca tgcgctctcc catggcgtaa
    31201 ccgacaattt cgcacgtata aacatctttg acgccagcga gatacagcca tccttcccgg
    31261 gtggcaacat acgtta
//
LOCUS       DBBBSB010000036        31191 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.036, whole genome shotgun sequence.
ACCESSION   DBBBSB010000036 DBBBSB010000000
VERSION     DBBBSB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31191
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.036"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            393..2051
                     /locus_tag="ACIUS0_001672"
     CDS             393..2051
                     /locus_tag="ACIUS0_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGB0219458.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="ACIUS0_001673"
     CDS             complement(2048..3004)
                     /locus_tag="ACIUS0_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGB0219459.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="ACIUS0_001674"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="ACIUS0_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGB0219460.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="ACIUS0_001675"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="ACIUS0_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGB0219461.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="ACIUS0_001676"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="ACIUS0_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGB0219462.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6660..6734
                     /gene="yljB"
                     /locus_tag="ACIUS0_001677"
     CDS             6660..6734
                     /gene="yljB"
                     /locus_tag="ACIUS0_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGB0219463.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="ACIUS0_001678"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="ACIUS0_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGB0219464.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="ACIUS0_001679"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="ACIUS0_001679"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGB0219465.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFEQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9863..9950)
                     /locus_tag="ACIUS0_001680"
     tRNA            complement(9863..9950)
                     /locus_tag="ACIUS0_001680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9914..9916),aa:Ser,seq:gga)
     gene            complement(10204..10422)
                     /gene="infA"
                     /locus_tag="ACIUS0_001681"
     CDS             complement(10204..10422)
                     /gene="infA"
                     /locus_tag="ACIUS0_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGB0219466.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10707..11411)
                     /gene="aat"
                     /locus_tag="ACIUS0_001682"
     CDS             complement(10707..11411)
                     /gene="aat"
                     /locus_tag="ACIUS0_001682"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGB0219467.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="ACIUS0_001683"
     CDS             complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="ACIUS0_001683"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGB0219468.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="ACIUS0_001684"
     CDS             complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="ACIUS0_001684"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGB0219469.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="ACIUS0_001685"
     CDS             complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="ACIUS0_001685"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGB0219470.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="ACIUS0_001686"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="ACIUS0_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGB0219471.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21230
                     /gene="ftsK"
                     /locus_tag="ACIUS0_001687"
     CDS             17202..21230
                     /gene="ftsK"
                     /locus_tag="ACIUS0_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGB0219472.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21385..21996
                     /gene="lolA"
                     /locus_tag="ACIUS0_001688"
     CDS             21385..21996
                     /gene="lolA"
                     /locus_tag="ACIUS0_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGB0219473.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22007..23350
                     /gene="rarA"
                     /locus_tag="ACIUS0_001689"
                     /gene_synonym="mgsA"
     CDS             22007..23350
                     /gene="rarA"
                     /locus_tag="ACIUS0_001689"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGB0219474.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23441..24733
                     /gene="serS"
                     /locus_tag="ACIUS0_001690"
     CDS             23441..24733
                     /gene="serS"
                     /locus_tag="ACIUS0_001690"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGB0219475.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24973..27416
                     /gene="dmsA"
                     /locus_tag="ACIUS0_001691"
                     /pseudo
     CDS             24973..27416
                     /gene="dmsA"
                     /locus_tag="ACIUS0_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27427..28044
                     /gene="dmsB"
                     /locus_tag="ACIUS0_001692"
     CDS             27427..28044
                     /gene="dmsB"
                     /locus_tag="ACIUS0_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGB0219476.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28046..28837
                     /locus_tag="ACIUS0_001693"
     CDS             28046..28837
                     /locus_tag="ACIUS0_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HGB0219477.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28873..29499)
                     /gene="ycaC"
                     /locus_tag="ACIUS0_001694"
     CDS             complement(28873..29499)
                     /gene="ycaC"
                     /locus_tag="ACIUS0_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGB0219478.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29814..30962
                     /locus_tag="ACIUS0_001695"
     CDS             29814..30962
                     /locus_tag="ACIUS0_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219479.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30980..>31191
                     /locus_tag="ACIUS0_001696"
     CDS             30980..>31191
                     /locus_tag="ACIUS0_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGB0219480.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7694 a         7645 c         8374 g         7478 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgaacaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9901 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9961 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    10021 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat
    10081 ctacatcgag acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata
    10141 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    10201 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    10261 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    10321 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10381 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10441 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10501 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10561 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10621 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10681 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc
    10741 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10801 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10861 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10921 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10981 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg
    11041 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    11101 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    11161 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    11221 cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca
    11281 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    11341 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11401 accaagcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11461 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct
    11521 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11581 gatgggtgac cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct
    11641 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11701 catgtaacag cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11761 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    11821 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11881 cacgcagtgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11941 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    12001 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    12061 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    12121 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    12181 gagtttgggt atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    12241 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    12301 caaacgcggc taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc
    12361 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    12421 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12481 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12541 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12601 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12661 gtaacataac gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12721 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    12781 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg
    12841 ccagtccggc aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca
    12901 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12961 tgattgcggc accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    13021 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    13081 tggcgagcag cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt
    13141 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    13201 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    13261 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    13321 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13381 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13441 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13501 cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13561 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13621 cccgcaattt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13681 atgattctgg tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13741 agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca
    13801 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13861 ccggcgacgt gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt
    13921 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13981 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    14041 aaaactctgg cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt
    14101 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    14161 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt
    14221 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    14281 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    14341 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg
    14401 ccataaacaa cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14461 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14521 gatagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    14581 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14641 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14701 agaccaccca tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14761 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14821 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14881 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca
    14941 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    15001 aaataaaggc gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta
    15061 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    15121 gccagtaccg gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc
    15181 aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    15241 gtagccgttt tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat
    15301 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15361 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15421 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15481 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15541 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15601 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15661 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15721 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15781 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa
    15841 cggaccggtc agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag
    15901 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15961 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    16021 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    16081 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    16141 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    16201 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    16261 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccacagca gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc
    19621 cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac
    19681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    19741 acaaaccgac gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    19801 agccggtaga tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg
    19861 atttccgtat taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg
    19921 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    19981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    20041 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    20101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    20161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    20221 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    20281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    20341 gcattccgca cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    20401 gctggtgtgt taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta
    20461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    20521 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    20581 catacattgt cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    20641 tggaagagct gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    20701 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    20761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    20821 gcgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc
    20881 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    20941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    21001 gtggtgcggg tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    21061 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    21121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    21181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    21241 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    21301 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    21361 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca
    21421 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    21481 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    21541 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    21601 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    21661 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    21721 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    21781 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    21841 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    21901 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    21961 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    22021 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    22081 agcacagtat atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    22141 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    22201 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    22261 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    22321 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    22381 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    22441 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct
    22501 gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta
    22561 tggtggtcag gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa
    22621 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta
    22681 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    22741 cgcccgcttt gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc
    22801 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    22861 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    22921 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    22981 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    23041 aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga
    23101 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    23161 cgggcaggaa tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    23221 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    23281 caagattggt gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    23341 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    23401 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    23461 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    23521 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    23581 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    23641 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    23701 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    23761 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    23821 taccccgcgt gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc
    23881 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    23941 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    24001 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    24061 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    24121 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    24181 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    24241 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    24301 cgacaaagtt gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga
    24361 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    24421 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    24481 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    24541 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    24601 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    24661 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    24721 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt
    24781 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    24841 gaaaatctac ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga
    24901 gctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt
    24961 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc
    25021 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    25081 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    25141 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    25201 gtcgtggacg gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg
    25261 gcctacacta ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc
    25321 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25381 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25441 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25501 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25561 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25621 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25681 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25741 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25801 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25861 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25921 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25981 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    26041 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    26101 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    26161 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26221 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26281 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26341 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26401 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26461 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26521 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26581 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26641 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26701 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26761 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26821 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26881 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26941 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    27001 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    27061 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    27121 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27181 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27241 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27301 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27361 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27421 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27481 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27541 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27601 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27661 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27721 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27781 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27841 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27901 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27961 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    28021 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    28081 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    28141 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28201 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28261 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28321 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28381 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28441 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28501 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28561 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28621 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28681 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28741 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28801 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28861 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28921 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28981 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    29041 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    29101 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    29161 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29221 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29281 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29341 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29401 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29461 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29521 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29581 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29641 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29701 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29761 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29821 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29881 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29941 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    30001 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    30061 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    30121 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30181 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30241 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30301 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30361 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30421 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30481 tgcctctgta cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg
    30541 tactggtcag tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg
    30601 gtcgactgct ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    30661 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    30721 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga
    30781 accaagcctt actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta
    30841 tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta
    30901 tctatttgct gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt
    30961 aaatgaattc aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta
    31021 tctgacttgc ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa
    31081 ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga
    31141 tgccccttta attttggcga aggatttgtc tatggctggg aatgttcagg a
//
LOCUS       DBBBSB010000037        30666 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.037, whole genome shotgun sequence.
ACCESSION   DBBBSB010000037 DBBBSB010000000
VERSION     DBBBSB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.037"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..588
                     /locus_tag="ACIUS0_001697"
     CDS             <1..588
                     /locus_tag="ACIUS0_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGB0219481.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="ACIUS0_001698"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="ACIUS0_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGB0219482.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="ACIUS0_001699"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="ACIUS0_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGB0219483.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="ACIUS0_001700"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="ACIUS0_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGB0219484.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="ACIUS0_001701"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="ACIUS0_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGB0219485.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="ACIUS0_001702"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="ACIUS0_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGB0219486.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="ACIUS0_001703"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="ACIUS0_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGB0219487.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="ACIUS0_001704"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="ACIUS0_001704"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGB0219488.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="ACIUS0_001705"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="ACIUS0_001705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGB0219489.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACIUS0_001706"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="ACIUS0_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGB0219490.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACIUS0_001707"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="ACIUS0_001707"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGB0219491.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACIUS0_001708"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="ACIUS0_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGB0219492.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACIUS0_001709"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="ACIUS0_001709"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGB0219493.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACIUS0_001710"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="ACIUS0_001710"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGB0219494.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACIUS0_001711"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="ACIUS0_001711"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGB0219495.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACIUS0_001712"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="ACIUS0_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGB0219496.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="ACIUS0_001713"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="ACIUS0_001713"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGB0219497.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="ACIUS0_001714"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="ACIUS0_001714"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGB0219498.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="ACIUS0_001715"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="ACIUS0_001715"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGB0219499.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="ACIUS0_001716"
     CDS             22192..22782
                     /locus_tag="ACIUS0_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGB0219500.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="ACIUS0_001717"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="ACIUS0_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGB0219501.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="ACIUS0_001718"
     CDS             23254..23949
                     /locus_tag="ACIUS0_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGB0219502.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACIUS0_001719"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="ACIUS0_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGB0219503.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="ACIUS0_001720"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="ACIUS0_001720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGB0219504.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="ACIUS0_001721"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="ACIUS0_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGB0219505.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACIUS0_001722"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="ACIUS0_001722"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGB0219506.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACIUS0_001723"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="ACIUS0_001723"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGB0219507.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7190 a         7982 c         8440 g         7054 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgattg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DBBBSB010000038        30298 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.038, whole genome shotgun sequence.
ACCESSION   DBBBSB010000038 DBBBSB010000000
VERSION     DBBBSB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30298
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.038"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACIUS0_001724"
     CDS             complement(<1..817)
                     /gene="wcaD"
                     /locus_tag="ACIUS0_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HGB0219508.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACIUS0_001725"
     CDS             complement(792..2009)
                     /gene="wcaC"
                     /locus_tag="ACIUS0_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGB0219509.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACIUS0_001726"
     CDS             complement(2006..2494)
                     /gene="wcaB"
                     /locus_tag="ACIUS0_001726"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGB0219510.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACIUS0_001727"
                     /pseudo
     CDS             complement(2497..3336)
                     /gene="wcaA"
                     /locus_tag="ACIUS0_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACIUS0_001728"
     CDS             complement(3429..5591)
                     /gene="wzc"
                     /locus_tag="ACIUS0_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGB0219511.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACIUS0_001729"
     CDS             complement(5594..6037)
                     /gene="wzb"
                     /locus_tag="ACIUS0_001729"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGB0219512.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACIUS0_001730"
     CDS             complement(6043..7182)
                     /gene="wza"
                     /locus_tag="ACIUS0_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGB0219513.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7491..7640
                     /locus_tag="ACIUS0_001731"
     CDS             7491..7640
                     /locus_tag="ACIUS0_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219514.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7841..9424
                     /locus_tag="ACIUS0_001732"
     CDS             7841..9424
                     /locus_tag="ACIUS0_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGB0219515.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACIUS0_001733"
     CDS             complement(9876..11729)
                     /gene="asmA"
                     /locus_tag="ACIUS0_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGB0219516.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACIUS0_001734"
     CDS             complement(11751..12332)
                     /gene="dcd"
                     /locus_tag="ACIUS0_001734"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGB0219517.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACIUS0_001735"
     CDS             complement(12424..13065)
                     /gene="udk"
                     /locus_tag="ACIUS0_001735"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGB0219518.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13383..13625
                     /locus_tag="ACIUS0_001736"
                     /pseudo
     CDS             13383..13625
                     /locus_tag="ACIUS0_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13624..14565
                     /gene="mdtA"
                     /locus_tag="ACIUS0_001737"
                     /pseudo
     CDS             13624..14565
                     /gene="mdtA"
                     /locus_tag="ACIUS0_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14565..17687
                     /gene="mdtB"
                     /locus_tag="ACIUS0_001738"
                     /pseudo
     CDS             14565..17687
                     /gene="mdtB"
                     /locus_tag="ACIUS0_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17688..20753
                     /gene="mdtC"
                     /locus_tag="ACIUS0_001739"
     CDS             17688..20753
                     /gene="mdtC"
                     /locus_tag="ACIUS0_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGB0219519.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20754..22169
                     /locus_tag="ACIUS0_001740"
     CDS             20754..22169
                     /locus_tag="ACIUS0_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219520.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22166..23569
                     /gene="baeS"
                     /locus_tag="ACIUS0_001741"
     CDS             22166..23569
                     /gene="baeS"
                     /locus_tag="ACIUS0_001741"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGB0219521.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23566..24288
                     /gene="baeR"
                     /locus_tag="ACIUS0_001742"
     CDS             23566..24288
                     /gene="baeR"
                     /locus_tag="ACIUS0_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGB0219522.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24479..24811
                     /locus_tag="ACIUS0_001743"
     CDS             24479..24811
                     /locus_tag="ACIUS0_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGB0219523.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24958..26319
                     /gene="yegQ"
                     /locus_tag="ACIUS0_001744"
     CDS             24958..26319
                     /gene="yegQ"
                     /locus_tag="ACIUS0_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGB0219524.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACIUS0_001745"
     CDS             complement(26661..26978)
                     /gene="yegR"
                     /locus_tag="ACIUS0_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGB0219525.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27384..28283
                     /gene="yegS"
                     /locus_tag="ACIUS0_001746"
     CDS             27384..28283
                     /gene="yegS"
                     /locus_tag="ACIUS0_001746"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGB0219526.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28365..29138)
                     /locus_tag="ACIUS0_001747"
     CDS             complement(28365..29138)
                     /locus_tag="ACIUS0_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGB0219527.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="ACIUS0_001748"
     CDS             complement(29244..30284)
                     /gene="gatD"
                     /locus_tag="ACIUS0_001748"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGB0219528.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7011 a         8048 c         7829 g         7410 t
ORIGIN      
        1 tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca ccaacccgga
       61 atacagccag agaaatcaat gctaacggta attttttctt aatcgcctct ttattcagat
      121 attgaaatgc ccactccagc aaataaaaca ggataaaggt cataacccct gaaaacgatc
      181 cggataatat tatccctgcg agaatcaggg catctgtttt aggcgttttg ataccaaact
      241 gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt tcgaaataaa
      301 gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa
      361 tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata atttgcgcca
      421 tctccaccgc cgccagcgcc accaccagtc cgaccaccag ataaaagaaa cgtaatatct
      481 tacgatggtt gtgtggcgaa attgttttaa aacgaatact ccacaccatg ccaataatga
      541 tcacaatata gacaaacagc atagttgaag tgacgtattt accggcatcc aacgactgac
      601 caaacaggta gttaaacgcc gtgagtcccg cgccaatccc taaggcaatc atcaattttt
      661 taatgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg acgatggtga
      721 tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag atcagtggca
      781 gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat ctgttgtcca
      841 ctgtaggcgg cgcgactgcg ttggctgaac tcagccagcg tggtaccaaa tatcgcctgc
      901 gcgatttccg gtttgcttaa ctgtaccagt tgcagcacct cttcttcact gacggtttta
      961 ccaccggatt tttgcagcac ttcccgcgcc gcatcgctat gggtggcaat caccggcacg
     1021 ccaatcgata gcgcctcaca caaaatcagc gggtagttat cgacacgaga actgaacacc
     1081 agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga
     1141 ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag ttcgattttg
     1201 tcgcccagcg ccatcatctc acgcaccagt tgctggttag ttttgccgtc gtaacgcagg
     1261 tcatgcgcca ccactgcgat tttcggcttg ccctgagttt cgcgcaccgg aggcagatct
     1321 gccagaatcg cttcggttgc catatcaatg ccattattga taatccggca acgccctggc
     1381 ccgtacagac tattgaaagc gtcagccaca tgctggctgg gggaaataaa ctgacagccc
     1441 agcgccagca tctcgcggaa taactggcgt ttgcccgcca ccagctggtg cgcgcgatca
     1501 atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt tttccagcct
     1561 tcgcaaccgt cggtaaaggc gcagcgtccg gttacactcc agtggtcgtg cagcgtccag
     1621 accagggtga catccggttt atggtttttc actttttcgc aaaagcgcac cacgctgtta
     1681 agattcaacc agtagctgtg cagcacatga aaatgcagga ccaccggacc cggtgtgcga
     1741 gtaatggtgc gatataactc attgaaattg ccgaacagat cgcgattaaa cagacgaaac
     1801 aacgcaatgt tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc
     1861 tgatggctga cgctctcttt gccgcctttg ccgtagccgt agacaaaatg tgacgccagc
     1921 ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc gccttccgcc
     1981 agtcgcacat taaattgcag aatattcatt taatcacctt cactcgcgct ttttctccca
     2041 ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc acggtgacgt
     2101 tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg acgccgttgc
     2161 caatgtgtgg acatgccatg ttatcggcac cccgattgcc gatagtgacg ccgtggcgaa
     2221 tggtgaaatc atcccccgcc accacgtttt tattgatcac tacggcgtaa ccgtgatgga
     2281 tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg
     2341 tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg acgttctttt
     2401 tgcgccacac cgaacaaaaa tgagcgacgc gataggcaag taccatgcag cacgggcgta
     2461 aactccagct gttggcgcgc agatcttcca gcatctttag cgcccccgaa taccatcagc
     2521 cagacgtttg ccgttacgca ccgacagcag cgtcaacaag gtgcgccagg tcatacgttt
     2581 attgcggatc tggtagaggg taaacagctg atatttttta ctggcgcggt cgaatttgtc
     2641 tttgtgcttg cggtaaaagt ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg
     2701 catttcgccg tgattgatat gcaggatctg cgtcgcctct tccactttcc acggttcgcc
     2761 gtactccacc accatccgca ggaatatgtc gtaatcctgt gctgctttca gttcggtatc
     2821 gaacaaacac tctttgaaac gccatgccca ggtaaagacc tgattgccaa tgatattacg
     2881 tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc tcgccggttg
     2941 ggaataaact tctccctagc aaacgtagtc gttagcgtac aaaaaggcgt gtgtgaccag
     3001 ttgctgttta tgggcgagga agacgctcag gcggttgggc gtccattcat catcatcatc
     3061 gatcccggtg atgtattccc cttgcgccag cataatcgcc tggttacgca ccgcgcacgc
     3121 cccgctgtta atgtcgttgt ggatgtaagt gatacgcgga tcgttgaggg cagtgacgta
     3181 ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct cccagttgct
     3241 gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc ggttccaggt
     3301 tggcatatag attgagatca gcggattgtc tttcataggt tgttctcccg gcttatgagc
     3361 tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg gtgcgaatgc
     3421 aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata atcctgatac
     3481 gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat accgttttgc
     3541 tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata acgcgccacc
     3601 attaacgtgg ttccgacatg acgaccaaca attgccgcat cggtcactgc cagaatcggc
     3661 ggcgtatcaa tcaacaccag gtcatagttt ttactcgccc agttcaccag ttcggcaaag
     3721 cgttcgctca ttaacagttc agaaggattt ggcggcacct gaccgcgcgg gatcaggtca
     3781 aatttggcaa tagaggtcgg tttagcagcg gtagtaatat cgccctgacc aatcagaatc
     3841 tccgacagcc cattgacatt attggtgccc aacagctcgt gggtgtagcc tttgcgcata
     3901 tcgcagtcga tcaacaacac gcgtttattg gtctggctga tcaccgccgc caggttggcg
     3961 cagacaaagg ttttaccgat tgacgggcta accccggtca tcatcaacac attgttctgc
     4021 gcctgcatca tcgcgaagtg cagactggta cgcagactac ggatggcttc aatcgccaga
     4081 tcggttggat tccccactgc cagtagctgg ctctgtttat agcgtttaac ccctttgatg
     4141 gttttgacgc tatcgcgcgc tttctgccac tccgacagcg ggatgctggc atagacgctg
     4201 attccgtgtt cttccagcac ttgcgggctt tcgatgccgc gattaaacaa cgagcgcagc
     4261 agcacaccca cgatagagag catcaggcca aggataatcg cgccgaggat aatcagccct
     4321 ttcttcggtt ttagcacgcc aggctgggtg attgccgggt caataatgcg cacatcgccg
     4381 acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg catatagacc
     4441 tgctgaccag actcgacatc gcgggtcaga cggacaattt cctgctggtt tttcggcatc
     4501 gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg tttctccagc
     4561 agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc ctctttaaag
     4621 gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc tttcgcttca
     4681 agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt ttcggcaaca
     4741 tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct tttcgacgct
     4801 tccgccgatt tgcgctcaat attttgttcc tgatagttac gagcgatgct gttaagaatg
     4861 tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg
     4921 ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa cgtggagtat
     4981 ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg
     5041 ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc atcactgctc
     5101 agggtgtagt ttttgtcatc cagcacatta agcgtaaaca cctgatccgc catctctttc
     5161 gggcggttaa aggtagtcac tttcaccgtc tcgttctgac gccccatcag gcgatcccag
     5221 cccgccccga aaatcgggaa cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc
     5281 accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc cgatgcaggc
     5341 ggtttgttgg ctaatgccga gccgatatcc tgcaccagcg aattgccgct gttttgctcg
     5401 atttgtacca gtgcgtcggc actataaatc ggcgtggcga agaaagtgta aaccacggca
     5461 cagagggcaa atacgacggt gatgccaatc acccaccagc gcgcttcaat gacggtgccg
     5521 accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg ttgttttact
     5581 ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc
     5641 gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgtgatac ggatcgggga
     5701 tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc tccggtgcca
     5761 tctcgcataa gcgttcgata tggcgcttat ccatggtcaa aatcaggtca tagttgcgac
     5821 acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga tgttctgcgg
     5881 cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt ccagcggact
     5941 ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccatcggg gaacggcaaa
     6001 tattgccgac acagacaact aagatgttgt taaacatgac gattaccagt tatgaatgtc
     6061 gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcagcaact gattgatcag
     6121 acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag gttgcaggcg
     6181 gaattctgtc gccatcacca gcgacgtcgc atcggacata tccagctggt agatattggc
     6241 aatcttgccg ttacgcccgc cctcgccttt cagcggacga atgacaaaga tgccgctggc
     6301 gttggaggtg gtcatgtcga taccttcggc attggccaga gcttcagtca gggtcatgcc
     6361 gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa atactttcag
     6421 atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt tctgattgag
     6481 gtcgccgttt tgcatcagcg cctgtaaaga aatgcgttct tcacgaccat tgtgtgttag
     6541 caccacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga tggcgtcgag
     6601 aatggtcagt ggtacgttgg tgatcgcctg ttgaccggat ttattcacct gacctgagat
     6661 ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt cagcgatgta
     6721 cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc cgactacgtg
     6781 aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc
     6841 gctggagctg cggtactgac cagctggcgt ggtgagttcc gggtgatccc agacggtgac
     6901 attaagaacg tcccccggcc cgacgcgata ctggtaattc gcgatctcgc tttccagcgt
     6961 catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag
     7021 cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc
     7081 tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta tcaaggttac
     7141 tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt tatcactttg
     7201 gcagagtaat tatcctgtgc actattaata gcaatgtcgc catgcacatt taccttgcag
     7261 ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca tccgcaggct
     7321 attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg cccctggctt
     7381 tttagctacc aatacactga ttgagcttaa tttttcaaac cctctctgca tacaatcgtt
     7441 gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt atggtaattg
     7501 aatattggct cgccaataat gcaggaggaa gtgttacagc taacggaata gcaggcaagg
     7561 taacaattcg gcaattggct atttttaaga attatattaa gtatcattca atatggtttt
     7621 taggagtttc tttaggttga caatatttaa tattgcgtct ccacatgcga tatttcttaa
     7681 ataatgtttt attattaccg cttttaattc agggataatg tgaggttatt accctcaaat
     7741 aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc atgatcgaat
     7801 tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga ttgccgatcc
     7861 gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa
     7921 cctggtcttt attgccatcc tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg
     7981 ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctagcgt caatctcctg
     8041 gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta gcgcccgcga
     8101 cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg agctgaacga
     8161 acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga agttctgggg
     8221 cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag tgattaccgc
     8281 cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg ctatcagcct
     8341 gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga ccatcgttat
     8401 tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag gtttcggctt
     8461 cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga tcgaggcact
     8521 caatcagttg gctatcttta accggcgacg ttttctttcc gctaatcaga cgctacgcca
     8581 gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg cggaactgga
     8641 cgccaaaacc gcgtccatgc tggtggatca tggaaaccag cagatcttta atccgcagga
     8701 acggcggatg attgagcggg tacttaatct taaccagcgt accgtaagca gcattatgac
     8761 gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa tccgccagtt
     8821 gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg cagaagattt
     8881 gctcggtgtt gttcacgtta tcgacctgct acaacagtca ctgcgcggcg aaccgctcaa
     8941 cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt tgttaccagc
     9001 cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg agtttggctc
     9061 ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgccggta acttaccgaa
     9121 cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg gttcctggac
     9181 ggcgaatggt catatgccgc tggaggatct ggtgcaatat gtgccgctgc cgctggatga
     9241 aaaacgtgaa tatcacacca ttgccgggct gttgatggag tatttgcagc gtattccaaa
     9301 acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg tggaaagcca
     9361 tcgcgtgcag aaggtgcaga ttataccgct gcgtaaggat ggcgagatgg agtacgaggt
     9421 gtgatggtat cggccatttt acccaatgct gcgtaacgcc ttataacttc agcaatgaag
     9481 cgttgtctgt ggtaaacgga catcacagcg ggggtgtggg tccggctgta aatcgccgaa
     9541 tcgtttcctg taggctgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg
     9601 atgttacctc gcgcccgtac ccgatagccg aaaggaataa gatgagggaa ccgcgccagc
     9661 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
     9721 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
     9781 atcttacata ttcagaatat aatagtgccg ccgcgaacgc ctgatgcgac gctttacgcg
     9841 tcttatcagg cctacaggac aggtcacctg gtaacctaca tcttctccag caacttcttc
     9901 acatctttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
     9961 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    10021 tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc aatcagtttg
    10081 ctttccccgt tccagccacc cacgacccga atatcaaact gggtgtcgca ggtttgatct
    10141 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    10201 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    10261 acgttatcga agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    10321 atcatctgct ggaagttcat cccttccata cgtgtgtcgg tcatctcaac atgcgcctgt
    10381 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    10441 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    10501 tcaacgttct ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    10561 gtgcccggca gtgaaacctt tccaccgtta agcttgccct gcagttgagt aatttccagc
    10621 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    10681 acgttactgg cctgcaacaa aatatcagct gtaaagcctt gcagtccctg ataggccggt
    10741 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    10801 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    10861 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgt
    10921 ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag gcgtttatgt
    10981 gactcctgcc actgggactg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    11041 gcaccctgca actgccagtt aatttgttca atagccgctg ttaaatcatg cggataatca
    11101 gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc gctgatcgcg attaacccgc
    11161 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    11221 cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc cgccacctta
    11281 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    11341 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    11401 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    11461 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    11521 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    11581 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    11641 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaagagt
    11701 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    11761 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    11821 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    11881 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    11941 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    12001 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagtgtcacc
    12061 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    12121 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    12181 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    12241 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    12301 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc acatcactcc
    12361 cttaacgcca attacgtcaa gggcatacca acttatcact gaacggtaca caatttatca
    12421 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    12481 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    12541 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    12601 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    12661 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    12721 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    12781 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcaacag
    12841 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    12901 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    12961 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    13021 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    13081 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    13141 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgaaaaag
    13201 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    13261 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    13321 ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg gataaagcgg
    13381 taataagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    13441 tcagtttagg tttagtctcg tttatcttta cccttgtctc gcttgagctt tcgcagtttg
    13501 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    13561 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    13621 ccattccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc
    13681 cagttcaaag ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt
    13741 gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc
    13801 cgtcaggagc tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct
    13861 gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca
    13921 gtcgatggtc gcgttggtct caagcaagtt gatgttggta accaaatctc cagtggtgat
    13981 accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg
    14041 gaaagcgata tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa
    14101 gcctgggatc gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac
    14161 caaatcgatg ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat
    14221 gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc
    14281 gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg
    14341 aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa
    14401 gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc
    14461 ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag
    14521 aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc
    14581 cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc
    14641 tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc
    14701 tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg
    14761 atgtcatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc
    14821 tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc
    14881 taacattacc gctcaatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca
    14941 acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc
    15001 cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata
    15061 tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc
    15121 tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc
    15181 tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa
    15241 aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat
    15301 ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg
    15361 gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca
    15421 aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg
    15481 ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg
    15541 tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg
    15601 aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc
    15661 cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta
    15721 tggtgtttct cgatttttca atcaataacc tgacactgat ggctttaact atcgccaccg
    15781 gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag
    15841 gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct
    15901 cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg
    15961 ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc tcagcggtgg
    16021 tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta
    16081 aacagaacag cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg
    16141 gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg gcactcagca
    16201 cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc ccggtacagg
    16261 acaacggcat tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg
    16321 cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga
    16381 cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca
    16441 acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa
    16501 cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gacctgacca
    16561 tcgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg tcactggatg
    16621 cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg
    16681 atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca
    16741 gcgccagccg tctggggatc agcatggcgg acgtcgataa cgccctgtac aacgcgtttg
    16801 gtcagcgact gatttccact atttatactc aggctaacca gtatcgcgtg gtgctggagc
    16861 acaataccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg accagcagcg
    16921 acggtggcgt ggtgccgtta agctcaattg ccaaaattga gcagcgtttt gcgccgcttt
    16981 ccatcaacca tctggatcag ttcccggtaa caaccatctc ctttaacgtg ccggataact
    17041 attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg
    17101 tggatatcac cacgcaattc tagggcagca ccctcgcctt ccagtcggcg ctgggcagca
    17161 ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg
    17221 agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg gtcggcgcgc
    17281 tgctggcatt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt
    17341 tgctgatcgg tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg
    17401 agcgcgagca aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc
    17461 gtccgatcct gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta
    17521 ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg
    17581 tcagccaggt gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg
    17641 cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg
    17701 ccctcttcat ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg
    17761 gcatactggg tttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga
    17821 ttatggtcag cgcctcgctg cccggtgcgt cgccggagac aatggcgtct tccgttgcca
    17881 cgccgctgga gcgctcactt gggcgcattg ccggggttag tgaaatgacc tccagcagtt
    17941 cgctcggcag cacgcgcatt attttgcagt ttgattttga ccgggatatc aacggcgcag
    18001 cgcgtgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca
    18061 gccgcccgac ctatcgcaaa gcgaacccgt cggatgcgcc aattatgatc ctcacgctga
    18121 catccgatac ttattcgcag ggtgaactgt acgatttcgc ctcgacgcag ctggctccga
    18181 cgatttcgca aatcgacggt gtcggtgatg tcgatgtcgg tggcagctcg ctgcccgccg
    18241 tgcgcgtcgg gctgaccccg caggcgctgt ttaatcaggg cgtgtcgctg gacgacgtac
    18301 gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc
    18361 accgctggca gatccagacc aacgatgaac tgaaaaccgc cgctgaatat cagccgctga
    18421 ttattcacta caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgactgact
    18481 cagtgcagga tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttactgatga
    18541 tccgcaaact gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac
    18601 cagagttgca ggagaccatt ccggcggcga ttgatctgca aattgcccag gatcgctccc
    18661 ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg
    18721 tgattctggt ggtcttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg
    18781 cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca
    18841 ataatctttc gctgatggcg ctcaccattg ctactggttt cgtggtggat gacgccattg
    18901 tggtgctgga aaacattgcc cgtcatctgg aagcggggat gaaaccacta caggccgcgc
    18961 tgcaaggtac tcgcgaagtc ggctttacgg tgctgtcgat gagtctgtca ctggtggcgg
    19021 tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc gagtttgccg
    19081 tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc cctgacgcta acgccaatga
    19141 tgtgcggctg gatgctaaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg
    19201 gccgcatgtt ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc
    19261 atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc tcgatcccga
    19321 aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag gcggatcaga
    19381 gtatttcgtt tcaggcgatg cgcggcaaat tgcaggattt catgaaaatt atccgtgacg
    19441 atccggcagt ggataatgtc accggcttta ccggcggttc acgagttaac agtgggatga
    19501 tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac ggcgcagcaa attatcgacc
    19561 gtctgcgcgt gaagctggcg aaagaaccgg gggcgaatct gttcctgatg gcggtacaag
    19621 atattcgcgt tggtgggcgt cagtcgaacg ccagctacca gtacacgttg ttatccgacg
    19681 acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg acgttgccgg
    19741 aactggcgga cgtgaactcc gatcagcagg ataacggcgc ggagatgaat ctggtttacg
    19801 accgcgacac catggcacgg ctgggaatcg acgtacaggc cgccaacagt ctgttaaata
    19861 acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag tataaagtgg
    19921 tgatggaagt ggatccgcgc tatacccagg acatcagtgc gctggaaaaa atgttcgtta
    19981 tcaataacga aggcaaagcg atcccgctgt cgtatttcgc taaatggcaa ccggcgaatg
    20041 ccccactatc ggtgaatcat cagggattat cggcggcctc gaccatctcg ttcaacctgc
    20101 cgaccggaaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg acccagcttg
    20161 gtgtgccttc gacggtgcgc ggcagttttg ccggcacggc gcaggttttc caggagacga
    20221 tgaactcgca ggtgatcctg attatcgccg ccatcgccac ggtgtatatc gtgctgggta
    20281 tcctttacga gagttacgta catccgctga cgattctctc caccctgccc tcggcgggcg
    20341 ttggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatc gccctgatag
    20401 ggatcatgct attaatcggc atcgtgaaga aaaacgccat tatgatggtc gattttgcgc
    20461 ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag gcctgtctgc
    20521 tgcgttttcg cccgattatg atgaccaccc tggcggcgct gtttggcgcg ctgccgctgg
    20581 tattgtcggg cggtgacggc tcggagctgc ggcaacccct ggagatcacc attgtcggcg
    20641 gtctggtgat gagccagctc ctgacgctgt ataccacgcc ggtggtgtat ctctttttcg
    20701 accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac ggtaaccgag taaatgacag
    20761 atcttcccga cagcacccgc tggcaattat ggattgtggc tttcggcttc tttatgcagt
    20821 cgctggacac caccatcgta aacaccgccc ttccctcaat ggcgcaaagc ctcggggaaa
    20881 gtccgttgca tatgcacatg gtcattgtct cttatgtgct gaccgtggcg gtgatgctgc
    20941 ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt accgccatcg
    21001 tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac gaactgttgc
    21061 tggcacgcgc gttacagggc gttggcggtg cgatgatggt gccggtcggc aggttgacgg
    21121 tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc acgttacccg
    21181 gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct gctggtagaa tacgcgtcgt
    21241 ggcactggat ctttttgatc aacattccgg tggggattat cggtgcgatc gccacattga
    21301 tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctttccggc tttttattgc
    21361 tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt acaggtttat
    21421 cgccgctggc gattgctggc ctggtcgcag ttggcgtggt ggcactggtg ctttatctgc
    21481 tgcacgccag aaataacaac cgcgccctgt tcagtctgaa actgttccgc actcgtacct
    21541 tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg ttgcccttta
    21601 tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc ggactgatga
    21661 tgatcccgat ggtgcttggc agcatgggaa tgaagcgaat tgtggtacag gtggtgaatc
    21721 gctttggtta tcgtcgggta ctggtagcga ccacgctggg tctgtcgctg gtcaccctgt
    21781 tgtttatgac taccgctctg ttgggctggt actacgtttt gccgttcgtc ctgtttttac
    21841 aagggatggt caactcgacc cgtttctcct ccatgaacac cctgacgctg aaagatctcc
    21901 cggacaatct ggcgagcagc ggaaacagcc tgctgtcgat gattatgcaa ttgtcgatga
    21961 gtatcggcgt caccatcgcc gggctgttgc tggggctttt tggttcgcag catatcagcg
    22021 tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg gcgtttatca
    22081 tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa aatgtagcta
    22141 tttcgcggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta ccggcaaact
    22201 gtttctggcg attttcgcca cctgcattgt cttgctgatc agtatgcact gggcggtgcg
    22261 tatcagtttt gagcgtggct ttattgatta catcaaacac ggcaatgaac agcgattaca
    22321 gctgttaagt gatgcgcttg gcgagcagta tgcgcagcat ggtaactggc gcttcctgcg
    22381 caacaacgat cgctttgtct ttcagatcct gcgttcattt gaacacgata attcggaaga
    22441 taaacccggc ccgggtatgc cgccgcacgg ctggcgtacc cagttctggg tggtcgatca
    22501 aaacaacaaa gtactggttg gtccgcgagc gccgattcca cctgacggta cacggcgacc
    22561 cattctggtc aacggtgcgg aagtgggcgc ggtgattgcc tcccctgttg agcggctgac
    22621 ccgcaatact gatatcaatt tcgataaaca acagcggcaa accagctggc tgatcgtcgc
    22681 cctggcaacg ttactcgcgg cgctcgccac ctttctgctg gcgcgcggtt tgctggcacc
    22741 ggtaaaacga cttgtcgatg gcacgcacaa actggcggcg ggcgatttca ctacccgcgt
    22801 aacgcccacc agtgaagatg aactgggcaa actggcgcaa gacttcaacc agctcgccag
    22861 cacgctggag aaaaaccagc aaatgcggcg cgattttatg gccgatattt ctcacgaact
    22921 gcgtacgcca ttagcggtgc tgcgcggtga actggaagcc attcaggatg gcgtgcgcaa
    22981 attcacgccg gagacggtgg cgtctttaca ggcggaggtc ggtacactga ccaaactggt
    23041 tgacgatctt catcagttgt cgatgtctga tgaaggcgct ctcgcctatc aaaaagcacc
    23101 ggtagatttg atcccactgc tggaagtggc gggcggcgca tttcgcgaac gattcgccag
    23161 ccgtggcctg aaactgcaat tttccctgcc agacagtatc accgtatttg gcgatcgcga
    23221 ccgtttaatg cagttattca ataacttact ggaaaacagc ctgcgctaca ctgacagcgg
    23281 cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga cctttgccga
    23341 cagtgcgcca ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt tttatcgcac
    23401 cgaaggttcc cgcaaccgcg ccagcggcgg ttccgggctg gggctggcga tttgcctgaa
    23461 cattgttgaa gcccataatg gccgcattat tgccgcccat tcgccttttg gcggggtaag
    23521 cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac cgagttacca
    23581 atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct ggggcagttg
    23641 ctcattgatt atctgcgtgc tgcgagctat gcgccgacgc ttatcagcca cggcgatcag
    23701 gtactggcgt atgtgcgcca gacaccaccg gatctgatcc tgttggatct gatgctccct
    23761 ggcaccgatg gcctgacatt atgccgggaa attcgtcgtt tttccgatgt tccgatcgtg
    23821 atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggctggagat tggcgccgat
    23881 gactacatct gcaagccgta cagcccacgg gaagtggtag cgcgtgtcaa aaccattttg
    23941 cgccgttgta aaccgcaacg cgaacttcag caacaggatg ccgaaagccc gttgattatc
    24001 gacgaaggtc gttttcaggc ttcatggcgc ggtaaaatgc ttgacctgac gcctgcggaa
    24061 tttcgtctgc ttaaaacgct ctctcacgaa ccaggaaaag tgttctcccg cgagcaattg
    24121 ctcaatcatc tttatgacga ctaccgcgta gtaaccgacc gcaccattga cagccacatt
    24181 aaaaatctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat ccgcgccgtt
    24241 tacggcgttg gttaccgctg ggaagccgac gcctgccgca tcgtttagtt ttagcgacat
    24301 tactttgtta gccggagatg gcgttcagac ccgatccggc aacattattc cacgcatggt
    24361 cagcaaactg acattatctc tccgccctgg caggcatcag aaaatcgctc atactttaat
    24421 cagtaaacag cacctttaga tgctgttttc gataagcaat ttcaatcaag gagtcattat
    24481 ggctggttgg tttgaactca gtaagagcag tgataatcag ttccggtttg tgctaaaagc
    24541 gggcaatggt gagactatcc tcaccagcga gctgtatacc tcaaaagcct ctgcggaaaa
    24601 gggcatcgcg tcggtgcgta gcaacagccc gcaagaagag cgctatgaga aaaaaacggc
    24661 aagcaacggc aaattctatt tcaatctgaa agccgctaat catcaaatta tcggctccag
    24721 ccagatgtac gccaccgcgc aatctcgtga aaccggaatt gcctccgtta aagccaatgg
    24781 cacaagccag acggtgaaag acaatacgta attacgatgc cgggcgtggg ttgttggcgt
    24841 ccggcgcttt accagatccc ccctacagcg ctacaatgcc cgcccttaaa gtgggggcct
    24901 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    24961 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    25021 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctacgtgt gcgcaacaac
    25081 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    25141 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    25201 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    25261 atgctggtgc gtgagcactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    25321 gtgaactggg cgacggtgaa attctggcag caaatgggac tgacccgcgt gatcctctct
    25381 cgcgagctgt cgctggaaga gattgaagag atccataatc aggtgccgga tatggagatc
    25441 gagatcttcg ttcacggcgc actgtgcatg gcctactccg gtcgctgcct gctctctggc
    25501 tatatcaaca agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac
    25561 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatt
    25621 ccggtgcaaa atgttgagcc gacgctgggt atcggcgcgc caaccgacaa agtgtttatg
    25681 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    25741 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    25801 ggcgtgcatt cgctgaaaat cgaaggtcgt accaaatctt tctactattg cgcacgcacc
    25861 gcacaggttt accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc
    25921 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    25981 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    26041 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    26101 aaattctccg ttggcgacag tcttgagctg atgacgccgc aaggcaacat taactttacc
    26161 cttgagcaca tggaaaacgc caaaggcgaa gctatgccgg tcgcaccagg cgatggttat
    26221 actgtgtggc tcccggtccc gcaggatctt gagctaaatt acgcgctgct aatgcgtaat
    26281 ttctccgggg aaaccacgcg taacccccac ggtaagtgat taatttcgat tatttttccc
    26341 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    26401 ttcgctgaaa aacataatcc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    26461 tgtaatctcc cttacacggg cttatttttt gtacataagc tgtaaagaag gattgatccc
    26521 tgataaccgt cttccattga ccacatcgat agaatccccc ttcatagcac aatgcctttc
    26581 acgtaacggt atcgtgctcg cccaggttcc ggctacgcac agccagaacg cgcatgtttg
    26641 acgcttacaa aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    26701 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    26761 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
    26821 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    26881 gcaagaccaa atttaccgat acttgtttca tcattatgca cagcacaacc agatataaga
    26941 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaaatac
    27001 caacgacact ctctagtgtt taaatataat aatggcatga ttattataat tgaataggat
    27061 tataataaat gttctgtaca acatttccta cataagtagg aattacggac attgaggccc
    27121 ttcagggtaa ctccatgggg gctttaatat attatattga agatgccact gtttagttga
    27181 atattaggta tatgctcttt tttgaaattt atcggtggca gccgttagta ttcgctgtcc
    27241 ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga ctttttatcc
    27301 tccgactttc atcctgttct gactccacct tttgttttct gctctacact atctacagac
    27361 caatcataaa ggcacatacg atcatggcag aatttcccgc cagcttactg attcttaatg
    27421 gtaaaagtac tgacaatcta cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa
    27481 tgacgatcca tgtgcgggtc acctgggaga aaggtgatgc cgcacgattt gtagaggagg
    27541 cccggaagtt gggcgtcgca acggtgattg ccggtggtgg cgatggcacc attaatgaag
    27601 tttctacggc gttgattcag tgtgaggggg atgacatacc cgcgctggga attttgccat
    27661 taggaaccgc caatgatttt gccaccagtg tagggattcc tgaggcactg gataaggcgc
    27721 tgaaactggc aattgccggt aatgccattg cgatagatat ggcgcaggtc aacaaacaaa
    27781 cctgttttat taatatggcg acaggcggat ttgggacgcg tattaccaca gaaacgccgg
    27841 aaaaattaaa agccgcgctg ggtggcgtct cttacatcat tcatggctta atgcgcatgg
    27901 atactctgca accggaccgt tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg
    27961 ccctggtcat tggtattggt aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga
    28021 acgcgttaat taacgatggc ttgctgcaac tgcgcatttt taccggcgat gaaattcttc
    28081 cggctctcgt atcaacctta aaatctgacg aagataaccc gaatattatc gaaggcgctt
    28141 cgtcgtggtt tgatatacaa gccccacacg aaatcacttt taatcttgat ggcgaaccgt
    28201 tgagtgggca aaacttccat attgaaatac ttccggcggc gttgcgttgt cgattaccac
    28261 cagattgtcc attattgcgt taatcaaact attctctctt gaaccaggga gaatccgtaa
    28321 tgccggatgc ggtgtaaaca ccttatccgg catacagaac aatactacac cgtaattaat
    28381 tttacccgtg tcttctgcaa tgccaattta tccacctctg acacagcatc atcggtaaca
    28441 ataatgtcaa ttttttcgat tggcagtacc tgattaaaac cgcgacgatt aaatttcgat
    28501 gaatcgagaa cggcaatcac tttatttgcg gcggtgacca tcgccccgct aatcgaataa
    28561 ccttcattaa aggtcgtaat accattaacc gcatcaatac catcagcacc gacaaacatt
    28621 aaatcggcat taatatcctg caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt
    28681 ttatggcgga ccgtgccacc acagacgacc agagtaatgt ctttattttc ggaaagggca
    28741 aacgctgccg ggagactgtt ggtaatcacc gtgatatttt tagcggtcat taatccttcg
    28801 gcgataagca ttgtggtact tccgctgtcg agaataacgg tcatcccttc atggatcatt
    28861 ttgactgccg cctgcgctat tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta
    28921 aacccgactt cctgggtctc ggtttcacta ttaccggaca ttattttcgc cgcaccgcca
    28981 tgaaagcgcg taaccacgcc tttttgttcg agaaagcgca aatcggcacg gattgtcgct
    29041 tccgaggcag caaatactcc cgccagatcc tgaacaagca cggttccctg ttcattcact
    29101 aattggatga tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt
    29161 gtggctagtg taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc
    29221 cagcatcacg ctggcccgcg gtttcatgga atgagcaaca ctttgccctg catggcatta
    29281 cgagcgatgt cacgcaccgc ctgggtgaag ctttcaaagc ttccacggtg agcgattaat
    29341 ggctccaggc ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc
    29401 ggccaagggc tggaatagtt catccaactg ccgataaccg tcagctcttt acgcaatatt
    29461 ttgccaaacg ttgccgatgt taaatgcaga tcctgatgca acgtgcccac cagcgccagt
    29521 tgggcatgag gaccggcaat ctctaccgcc agttcgacag tttgcggtac gccagccgtc
    29581 tcgaggataa gctgattaaa gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc
    29641 atttcgctac tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct
    29701 gagctaatgt cgatcgccgt cacactcttt gctcccagcg cgacagcgca ctgaatagcc
    29761 agcaggccaa tggttccggc accaataata ataacgtttt tattctcaca accttgcgct
    29821 aaatgaaaag catgcaggcc aacggtaatc ggctcaataa aagccccatc ctcaataggc
    29881 atatccgtgg gtagagcaaa gacatttttt cgcttaacga caatatattc agcaaatcca
    29941 ccatcacgcc gcgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaaa
    30001 cactctggac aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccagggtgt
    30061 aaatcatcaa caccggatcc caccgcatca atatagccgc taaattcatg gcctaacgtt
    30121 attggataat aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc
    30181 gagctggcaa tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgcttttt
    30241 gcaacgcgca cgataccatc agtatcattc actactgatt tcataaaaac tcctgatt
//
LOCUS       DBBBSB010000039        30201 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.039, whole genome shotgun sequence.
ACCESSION   DBBBSB010000039 DBBBSB010000000
VERSION     DBBBSB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.039"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACIUS0_001749"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACIUS0_001749"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGB0219529.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACIUS0_001750"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACIUS0_001750"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGB0219530.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACIUS0_001751"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACIUS0_001751"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGB0219531.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACIUS0_001752"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACIUS0_001752"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGB0219532.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACIUS0_001753"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACIUS0_001753"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGB0219533.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACIUS0_001754"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACIUS0_001754"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGB0219534.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACIUS0_001755"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACIUS0_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGB0219535.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACIUS0_001756"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACIUS0_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGB0219536.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACIUS0_001757"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACIUS0_001757"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGB0219537.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACIUS0_001758"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACIUS0_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGB0219538.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACIUS0_001759"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACIUS0_001759"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGB0219539.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACIUS0_001760"
     CDS             complement(15665..16702)
                     /locus_tag="ACIUS0_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGB0219540.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACIUS0_001761"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACIUS0_001761"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGB0219541.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACIUS0_001762"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACIUS0_001762"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGB0219542.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACIUS0_001763"
     CDS             19444..19884
                     /locus_tag="ACIUS0_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGB0219543.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACIUS0_001764"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACIUS0_001764"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGB0219544.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACIUS0_001765"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACIUS0_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACIUS0_001766"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACIUS0_001766"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGB0219545.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACIUS0_001767"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACIUS0_001767"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGB0219546.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACIUS0_001768"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACIUS0_001768"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGB0219547.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACIUS0_001769"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACIUS0_001769"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGB0219548.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACIUS0_001770"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACIUS0_001770"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGB0219549.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACIUS0_001771"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACIUS0_001771"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGB0219550.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACIUS0_001772"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACIUS0_001772"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGB0219551.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACIUS0_001773"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACIUS0_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGB0219552.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7299 a         8420 c         7620 g         6862 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt tatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc a
//
LOCUS       DBBBSB010000040        30032 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.040, whole genome shotgun sequence.
ACCESSION   DBBBSB010000040 DBBBSB010000000
VERSION     DBBBSB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.040"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(131..1795)
                     /gene="glnS"
                     /locus_tag="ACIUS0_001774"
     CDS             complement(131..1795)
                     /gene="glnS"
                     /locus_tag="ACIUS0_001774"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGB0219553.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1998..3944)
                     /gene="nagE"
                     /locus_tag="ACIUS0_001775"
     CDS             complement(1998..3944)
                     /gene="nagE"
                     /locus_tag="ACIUS0_001775"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGB0219554.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4277..5077
                     /gene="nagB"
                     /locus_tag="ACIUS0_001776"
     CDS             4277..5077
                     /gene="nagB"
                     /locus_tag="ACIUS0_001776"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGB0219555.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5137..6285
                     /gene="nagA"
                     /locus_tag="ACIUS0_001777"
     CDS             5137..6285
                     /gene="nagA"
                     /locus_tag="ACIUS0_001777"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGB0219556.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6294..7514
                     /gene="nagC"
                     /locus_tag="ACIUS0_001778"
                     /gene_synonym="nagR"
     CDS             6294..7514
                     /gene="nagC"
                     /locus_tag="ACIUS0_001778"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGB0219557.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7562..8314
                     /gene="nagD"
                     /locus_tag="ACIUS0_001779"
                     /pseudo
     CDS             7562..8314
                     /gene="nagD"
                     /locus_tag="ACIUS0_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8365..8463)
                     /locus_tag="ACIUS0_001780"
                     /pseudo
     CDS             complement(8365..8463)
                     /locus_tag="ACIUS0_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8711..10375
                     /gene="asnB"
                     /locus_tag="ACIUS0_001781"
     CDS             8711..10375
                     /gene="asnB"
                     /locus_tag="ACIUS0_001781"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGB0219558.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10754..10830
                     /locus_tag="ACIUS0_001782"
     tRNA            10754..10830
                     /locus_tag="ACIUS0_001782"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10788..10790,aa:Met,seq:cat)
     gene            10839..10923
                     /locus_tag="ACIUS0_001783"
     tRNA            10839..10923
                     /locus_tag="ACIUS0_001783"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10873..10875,aa:Leu,seq:tag)
     gene            10947..11021
                     /locus_tag="ACIUS0_001784"
     tRNA            10947..11021
                     /locus_tag="ACIUS0_001784"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10979..10981,aa:Gln,seq:ttg)
     gene            11056..11130
                     /locus_tag="ACIUS0_001785"
     tRNA            11056..11130
                     /locus_tag="ACIUS0_001785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11088..11090,aa:Gln,seq:ttg)
     gene            11146..11222
                     /locus_tag="ACIUS0_001786"
     tRNA            11146..11222
                     /locus_tag="ACIUS0_001786"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11180..11182,aa:Met,seq:cat)
     gene            11271..11345
                     /locus_tag="ACIUS0_001787"
     tRNA            11271..11345
                     /locus_tag="ACIUS0_001787"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11303..11305,aa:Gln,seq:ctg)
     gene            11383..11457
                     /locus_tag="ACIUS0_001788"
     tRNA            11383..11457
                     /locus_tag="ACIUS0_001788"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11415..11417,aa:Gln,seq:ctg)
     gene            complement(11611..12786)
                     /gene="ubiF"
                     /locus_tag="ACIUS0_001789"
     CDS             complement(11611..12786)
                     /gene="ubiF"
                     /locus_tag="ACIUS0_001789"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGB0219559.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12932..14356
                     /gene="miaB"
                     /locus_tag="ACIUS0_001790"
     CDS             12932..14356
                     /gene="miaB"
                     /locus_tag="ACIUS0_001790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGB0219560.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14509..15549
                     /locus_tag="ACIUS0_001791"
     CDS             14509..15549
                     /locus_tag="ACIUS0_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGB0219561.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15546..16013
                     /gene="ybeY"
                     /locus_tag="ACIUS0_001792"
     CDS             15546..16013
                     /gene="ybeY"
                     /locus_tag="ACIUS0_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGB0219562.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            16103..16981
                     /gene="corC"
                     /locus_tag="ACIUS0_001793"
     CDS             16103..16981
                     /gene="corC"
                     /locus_tag="ACIUS0_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGB0219563.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            17006..18544
                     /gene="lnt"
                     /locus_tag="ACIUS0_001794"
     CDS             17006..18544
                     /gene="lnt"
                     /locus_tag="ACIUS0_001794"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGB0219564.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            18941..19849
                     /gene="gltI"
                     /locus_tag="ACIUS0_001795"
     CDS             18941..19849
                     /gene="gltI"
                     /locus_tag="ACIUS0_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGB0219565.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            20019..20759
                     /gene="gltJ"
                     /locus_tag="ACIUS0_001796"
     CDS             20019..20759
                     /gene="gltJ"
                     /locus_tag="ACIUS0_001796"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGB0219566.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            20759..21433
                     /gene="gltK"
                     /locus_tag="ACIUS0_001797"
     CDS             20759..21433
                     /gene="gltK"
                     /locus_tag="ACIUS0_001797"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGB0219567.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            21433..22158
                     /gene="gltL"
                     /locus_tag="ACIUS0_001798"
     CDS             21433..22158
                     /gene="gltL"
                     /locus_tag="ACIUS0_001798"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGB0219568.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            22276..23211
                     /gene="rihA"
                     /locus_tag="ACIUS0_001799"
     CDS             22276..23211
                     /gene="rihA"
                     /locus_tag="ACIUS0_001799"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGB0219569.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            23295..24965
                     /gene="hscC"
                     /locus_tag="ACIUS0_001800"
                     /pseudo
     CDS             23295..24965
                     /gene="hscC"
                     /locus_tag="ACIUS0_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(25025..26476)
                     /gene="djlC"
                     /locus_tag="ACIUS0_001801"
     CDS             complement(25025..26476)
                     /gene="djlC"
                     /locus_tag="ACIUS0_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGB0219570.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(26473..27180)
                     /locus_tag="ACIUS0_001802"
     CDS             complement(26473..27180)
                     /locus_tag="ACIUS0_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGB0219571.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            27282..27836
                     /locus_tag="ACIUS0_001803"
     CDS             27282..27836
                     /locus_tag="ACIUS0_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGB0219572.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(27846..29273)
                     /locus_tag="ACIUS0_001804"
     CDS             complement(27846..29273)
                     /locus_tag="ACIUS0_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGB0219573.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(29270..29977)
                     /locus_tag="ACIUS0_001805"
     CDS             complement(29270..29977)
                     /locus_tag="ACIUS0_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGB0219574.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT         7477 a         7711 c         7536 g         7308 t
ORIGIN      
        1 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
       61 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
      121 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
      181 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
      241 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
      301 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
      361 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
      421 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
      481 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
      541 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
      601 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
      661 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
      721 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
      781 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
      841 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
      901 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
      961 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
     1021 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
     1081 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
     1141 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
     1201 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
     1261 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
     1321 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
     1381 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
     1441 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
     1501 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
     1561 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
     1621 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
     1681 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
     1741 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
     1801 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
     1861 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
     1921 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
     1981 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
     2041 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
     2101 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
     2161 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
     2221 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
     2281 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
     2341 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
     2401 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
     2461 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
     2521 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
     2581 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
     2641 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
     2701 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
     2761 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
     2821 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
     2881 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
     2941 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
     3001 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
     3061 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
     3121 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
     3181 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
     3241 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
     3301 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
     3361 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
     3421 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
     3481 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
     3541 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
     3601 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
     3661 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
     3721 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
     3781 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
     3841 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
     3901 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
     3961 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
     4021 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
     4081 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
     4141 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
     4201 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
     4261 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
     4321 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
     4381 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
     4441 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
     4501 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
     4561 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
     4621 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
     4681 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
     4741 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
     4801 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
     4861 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
     4921 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
     4981 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
     5041 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
     5101 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
     5161 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
     5221 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
     5281 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
     5341 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
     5401 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
     5461 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
     5521 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
     5581 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
     5641 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
     5701 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
     5761 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
     5821 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
     5881 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
     5941 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
     6001 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
     6061 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
     6121 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
     6181 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
     6241 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
     6301 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
     6361 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
     6421 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
     6481 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
     6541 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
     6601 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
     6661 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
     6721 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
     6781 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
     6841 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
     6901 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
     6961 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
     7021 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
     7081 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
     7141 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
     7201 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
     7261 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
     7321 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
     7381 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
     7441 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
     7501 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
     7561 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
     7621 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
     7681 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
     7741 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
     7801 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
     7861 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
     7921 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
     7981 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
     8041 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
     8101 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
     8161 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
     8221 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
     8281 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
     8341 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
     8401 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
     8461 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
     8521 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
     8581 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
     8641 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
     8701 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
     8761 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
     8821 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
     8881 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
     8941 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
     9001 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
     9061 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
     9121 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
     9181 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
     9241 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
     9301 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
     9361 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
     9421 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
     9481 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
     9541 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
     9601 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
     9661 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
     9721 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
     9781 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
     9841 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
     9901 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
     9961 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    10021 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    10081 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    10141 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    10201 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    10261 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    10321 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    10381 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    10441 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    10501 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    10561 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    10621 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    10681 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    10741 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    10801 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    10861 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    10921 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    10981 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    11041 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    11101 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    11161 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    11221 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    11281 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    11341 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    11401 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    11461 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    11521 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    11581 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    11641 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    11701 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    11761 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    11821 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    11881 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    11941 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    12001 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    12061 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    12121 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    12181 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    12241 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    12301 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    12361 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    12421 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    12481 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    12541 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    12601 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    12661 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    12721 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    12781 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    12841 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    12901 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    12961 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    13021 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    13081 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    13141 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    13201 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    13261 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    13321 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    13381 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    13441 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    13501 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    13561 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    13621 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    13681 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    13741 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    13801 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    13861 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    13921 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    13981 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    14041 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    14101 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    14161 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    14221 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    14281 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    14341 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    14401 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    14461 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    14521 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    14581 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    14641 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    14701 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    14761 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    14821 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    14881 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    14941 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    15001 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    15061 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    15121 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    15181 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    15241 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    15301 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    15361 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    15421 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    15481 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    15541 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    15601 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    15661 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    15721 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    15781 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    15841 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    15901 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    15961 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    16021 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    16081 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    16141 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    16201 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    16261 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    16321 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    16381 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    16441 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    16501 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    16561 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    16621 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    16681 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    16741 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    16801 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    16861 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcaatggccg atagtcggcg
    16921 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    16981 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    17041 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    17101 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    17161 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    17221 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    17281 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    17341 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    17401 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    17461 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    17521 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    17581 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    17641 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    17701 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    17761 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    17821 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    17881 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    17941 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    18001 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    18061 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    18121 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    18181 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    18241 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    18301 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    18361 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    18421 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    18481 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    18541 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    18601 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    18661 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    18721 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    18781 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    18841 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    18901 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    18961 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    19021 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    19081 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    19141 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    19201 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    19261 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    19321 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    19381 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    19441 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    19501 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    19561 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    19621 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    19681 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    19741 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    19801 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    19861 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    19921 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    19981 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    20041 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    20101 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    20161 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    20221 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    20281 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    20341 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    20401 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    20461 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    20521 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    20581 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    20641 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    20701 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    20761 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    20821 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    20881 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    20941 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    21001 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    21061 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    21121 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    21181 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    21241 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    21301 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    21361 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    21421 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    21481 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    21541 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    21601 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    21661 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    21721 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    21781 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    21841 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    21901 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    21961 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    22021 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    22081 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    22141 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    22201 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    22261 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    22321 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    22381 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    22441 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    22501 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    22561 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    22621 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    22681 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    22741 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    22801 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    22861 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    22921 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    22981 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    23041 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    23101 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    23161 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    23221 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    23281 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    23341 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    23401 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    23461 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    23521 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    23581 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    23641 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    23701 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    23761 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    23821 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    23881 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    23941 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    24001 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    24061 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    24121 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    24181 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    24241 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    24301 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    24361 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    24421 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    24481 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    24541 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    24601 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    24661 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    24721 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    24781 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    24841 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    24901 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    24961 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    25021 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    25081 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    25141 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    25201 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    25261 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    25321 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    25381 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    25441 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    25501 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    25561 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    25621 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    25681 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    25741 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    25801 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    25861 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    25921 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    25981 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    26041 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    26101 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    26161 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    26221 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    26281 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    26341 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    26401 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    26461 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    26521 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    26581 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    26641 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    26701 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    26761 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    26821 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    26881 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    26941 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    27001 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    27061 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    27121 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    27181 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    27241 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    27301 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    27361 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    27421 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    27481 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    27541 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    27601 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    27661 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    27721 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    27781 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    27841 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    27901 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    27961 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    28021 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    28081 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    28141 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    28201 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    28261 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    28321 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    28381 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    28441 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    28501 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    28561 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    28621 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    28681 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    28741 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    28801 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    28861 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    28921 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    28981 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    29041 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    29101 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    29161 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    29221 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    29281 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    29341 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    29401 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    29461 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    29521 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    29581 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    29641 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    29701 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    29761 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    29821 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    29881 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    29941 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    30001 ttaatgaatg taaactacat ttcgaggctc ag
//
LOCUS       DBBBSB010000041        29920 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.041, whole genome shotgun sequence.
ACCESSION   DBBBSB010000041 DBBBSB010000000
VERSION     DBBBSB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29920
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.041"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            144..527
                     /gene="panM"
                     /locus_tag="ACIUS0_001806"
     CDS             144..527
                     /gene="panM"
                     /locus_tag="ACIUS0_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGB0219575.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(726..1829)
                     /gene="livJ"
                     /locus_tag="ACIUS0_001807"
     CDS             complement(726..1829)
                     /gene="livJ"
                     /locus_tag="ACIUS0_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGB0219576.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(2100..2954)
                     /gene="rpoH"
                     /locus_tag="ACIUS0_001808"
     CDS             complement(2100..2954)
                     /gene="rpoH"
                     /locus_tag="ACIUS0_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGB0219577.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(3199..4257)
                     /gene="ftsX"
                     /locus_tag="ACIUS0_001809"
     CDS             complement(3199..4257)
                     /gene="ftsX"
                     /locus_tag="ACIUS0_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGB0219578.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(4250..4918)
                     /gene="ftsE"
                     /locus_tag="ACIUS0_001810"
     CDS             complement(4250..4918)
                     /gene="ftsE"
                     /locus_tag="ACIUS0_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGB0219579.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(4921..6417)
                     /gene="ftsY"
                     /locus_tag="ACIUS0_001811"
     CDS             complement(4921..6417)
                     /gene="ftsY"
                     /locus_tag="ACIUS0_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGB0219580.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            6567..7163
                     /gene="rsmD"
                     /locus_tag="ACIUS0_001812"
     CDS             6567..7163
                     /gene="rsmD"
                     /locus_tag="ACIUS0_001812"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGB0219581.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            7153..7422
                     /locus_tag="ACIUS0_001813"
     CDS             7153..7422
                     /locus_tag="ACIUS0_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGB0219582.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(7425..7784)
                     /locus_tag="ACIUS0_001814"
     CDS             complement(7425..7784)
                     /locus_tag="ACIUS0_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGB0219583.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            7925..8551
                     /locus_tag="ACIUS0_001815"
     CDS             7925..8551
                     /locus_tag="ACIUS0_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGB0219584.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            8625..10823
                     /gene="zntA"
                     /locus_tag="ACIUS0_001816"
     CDS             8625..10823
                     /gene="zntA"
                     /locus_tag="ACIUS0_001816"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGB0219585.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(10925..11170)
                     /gene="tusA"
                     /locus_tag="ACIUS0_001817"
     CDS             complement(10925..11170)
                     /gene="tusA"
                     /locus_tag="ACIUS0_001817"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGB0219586.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            11391..12056
                     /locus_tag="ACIUS0_001818"
     CDS             11391..12056
                     /locus_tag="ACIUS0_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGB0219587.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            12129..12686
                     /gene="dcrB"
                     /locus_tag="ACIUS0_001819"
     CDS             12129..12686
                     /gene="dcrB"
                     /locus_tag="ACIUS0_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGB0219588.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(12690..13907)
                     /locus_tag="ACIUS0_001820"
     CDS             complement(12690..13907)
                     /locus_tag="ACIUS0_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219589.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            14041..15090
                     /locus_tag="ACIUS0_001821"
     CDS             14041..15090
                     /locus_tag="ACIUS0_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGB0219590.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15145..15732
                     /gene="acpT"
                     /locus_tag="ACIUS0_001822"
     CDS             15145..15732
                     /gene="acpT"
                     /locus_tag="ACIUS0_001822"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGB0219591.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            15843..17417
                     /gene="nikA"
                     /locus_tag="ACIUS0_001823"
     CDS             15843..17417
                     /gene="nikA"
                     /locus_tag="ACIUS0_001823"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0219592.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            17417..18361
                     /gene="nikB"
                     /locus_tag="ACIUS0_001824"
     CDS             17417..18361
                     /gene="nikB"
                     /locus_tag="ACIUS0_001824"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGB0219593.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            18358..19191
                     /gene="nikC"
                     /locus_tag="ACIUS0_001825"
     CDS             18358..19191
                     /gene="nikC"
                     /locus_tag="ACIUS0_001825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGB0219594.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            19191..19955
                     /gene="nikD"
                     /locus_tag="ACIUS0_001826"
     CDS             19191..19955
                     /gene="nikD"
                     /locus_tag="ACIUS0_001826"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGB0219595.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            19952..20758
                     /gene="nikE"
                     /locus_tag="ACIUS0_001827"
     CDS             19952..20758
                     /gene="nikE"
                     /locus_tag="ACIUS0_001827"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGB0219596.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            20764..21165
                     /gene="nikR"
                     /locus_tag="ACIUS0_001828"
     CDS             20764..21165
                     /gene="nikR"
                     /locus_tag="ACIUS0_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGB0219597.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(21285..21644)
                     /locus_tag="ACIUS0_001829"
     CDS             complement(21285..21644)
                     /locus_tag="ACIUS0_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGB0219598.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(21641..21916)
                     /locus_tag="ACIUS0_001830"
     CDS             complement(21641..21916)
                     /locus_tag="ACIUS0_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGB0219599.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(21975..23099)
                     /locus_tag="ACIUS0_001831"
     CDS             complement(21975..23099)
                     /locus_tag="ACIUS0_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0219600.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(23099..25834)
                     /gene="rbbA"
                     /locus_tag="ACIUS0_001832"
     CDS             complement(23099..25834)
                     /gene="rbbA"
                     /locus_tag="ACIUS0_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGB0219601.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(25831..26898)
                     /locus_tag="ACIUS0_001833"
     CDS             complement(25831..26898)
                     /locus_tag="ACIUS0_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGB0219602.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(26961..27074)
                     /gene="yhiY"
                     /locus_tag="ACIUS0_001834"
     CDS             complement(26961..27074)
                     /gene="yhiY"
                     /locus_tag="ACIUS0_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGB0219603.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(27264..28886)
                     /locus_tag="ACIUS0_001835"
     CDS             complement(27264..28886)
                     /locus_tag="ACIUS0_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGB0219604.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(29148..>29920)
                     /locus_tag="ACIUS0_001836"
     CDS             complement(29148..>29920)
                     /locus_tag="ACIUS0_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGB0219605.1"
                     /translation="TRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYIL
                     TLLNSMRNMEGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLD
                     VCNYNSETKVLTSHHGIIRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQH
                     MDDHLLHLIRSNAIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGV
                     DQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT         6933 a         8108 c         7558 g         7321 t
ORIGIN      
        1 atttttcatt ctctaatgtt ttaactttgt aattattgct gttaaaaaat taatcacctg
       61 ccaaaagaaa taaaaaagag aaagcctccg attaaattat ttcgctacac tggttccact
      121 tttgtgattt acacgggtta cccatgaagc tgaccatcat tcgattagaa aactttagcg
      181 accaggaccg gattgacctg caaaagatct ggccggagta ttccccttcc tctttacagg
      241 ttgacgataa ccaccgtatc tacgccgcgc gttttaacga gcgtctgctt gcggccgtgc
      301 gggtaacctt aagcggcacc gagggagcac tggattctct acgcgtgcgg gaagtcaccc
      361 gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt gcgtaacaat cctggcgttt
      421 catgctggtg gatggcggat gcaggcgtgg aagatcgcgg tgtgatgacg gcgtttatgc
      481 aggcactggg atttacggca cagcagggcg gctgggagaa gcgttaatcg tcaagttgat
      541 ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta cgcttatcag gcctataatg
      601 tgtgctgcaa tttactgatt tctttggttc ttgtaggccg gataaggcgt ttacgccgca
      661 tccggcatga agcaacgtat tcgatattag caatttggcg gcaacccaaa gttgccgatt
      721 aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc agtcaaatac gccgaactca
      781 aagcccttta gatcgccttt ctcatcccag gtcagtggcc ccatcacggt atccacggag
      841 ttcgctttta ggtatttggc gatttcagcc ggatcgtcag actgattcag gcccgcctgc
      901 aaagattgca gcgcggcgta ggtggtccag acgaatgcgc cgcttgggtc ctgttttttc
      961 gctttgatcg catcaacaat gggtttgttc gccggaacct gatcgtagtt cttcggcttg
     1021 gtcaccagca gcccttccgc tgattcgccc gcaatgttag acagcgaaac gttagccaca
     1081 ccttccggcc ccataaactg agttttcagc cctgccgcgc gtgcctgacg caggatttgc
     1141 cccatttccg ggtgataacc gccgtagtaa acgaagtcga tattctcttt tttcagacgc
     1201 gccaccagcg ttgagaaatc tttttccccg gcggtgatac catcaaagaa caccacgtta
     1261 gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca gaccttcgcc gtattgctgt
     1321 ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct caagaatata tttcgccgcc
     1381 gtcggcccct ggtcggagtc caggccggtg gtgcgcagga tcagctgata gccacgggcg
     1441 gtcagctccg gcgcggttgc cgctggggtg atcattaaaa tgccttcgtc ttcgtagatg
     1501 tcagacgcag gctgcgttga tgaagaacag aggtgaccaa tcacatattt aatgccgtcg
     1561 ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt cacaggcatc gtcatatttt
     1621 acgatttgca gtttgttgcc tttaatgccg cctttagcgt tgatatccgc aaccgcctgc
     1681 tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg gaccggacat tgcacccacg
     1741 accgcgactt taatatcttc tgccagagcc atattgctga atgccagcgc gatacatcct
     1801 gccagtaacg ctttaccctt tatgttcatc ctgagaatcc ccattcttct ggttattacg
     1861 tgtgttgtga tgttgttgtt cagcacttta tttcgtttta tgcatgacta cccgtgcttt
     1921 agcagcatac tctgctaaaa catacccgat ttttatgata ttggaatagc tattttgaca
     1981 gtttattaac aatctgcgtg gggattggcg ttttgccgga gggggaattg attattgtag
     2041 aggcccaaaa aacaaaaacc ccggactctc atccagggtt ctctgcttaa tagcggaaat
     2101 tacgcttcaa tagcggcgcg aagttttttc atcgcgttct tttccagttg gcgcacacgt
     2161 tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg tggacttgtt gtcttcgtcc
     2221 agccagcgcg cacggatgat gtcctggctg cgttcgtcca gaccatgcat cgcgtcggtc
     2281 agacggtttg ccgcctgctc ttcccagtta tcatcttcga tgccgtcggc aaagttagat
     2341 gatttatcct gcagatagag caccggtgcc atcggctggc tgtcggaatc gtcgtcggaa
     2401 gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt ccatctcacg tacgtctttg
     2461 ctggttacgc ccagttcacg ggccaccatt tcgacttcat cctggttaaa ccagcccaga
     2521 cgctgcttgg ttttacgcag gttgaagaac agtttgcgct gcgctttggt ggtcgcaact
     2581 ttgacgatac gccagttacg cagaacgtat tcgtggatct ctgctttgat ccagtggacg
     2641 gcgaaggaga ccaggcgcac acccacttcc gggttaaaac ggcgcactgc tttcatcagg
     2701 ccgatgttac cttcctgaat caaatccgcc tgtggcaggc catagcccgc ataattacga
     2761 gcaatatgaa caacaaaccg caggtgagac aggatcagcg ttttagctgc ttccagatcg
     2821 ccatggtaat gcagcttttc agccagcgcc cgctcctcgt cagccgacaa catcggccac
     2881 gcgttagctg cccggatgta ggaatccagg ttgccaactg gggctaaagc taaactttgc
     2941 attttgtcag tcattcaaat cctctcaatc gatatcttct ggcgcttcag tggtagcaac
     3001 aactgtgcca aagcttaaga gcaacgaggt tatcattcac tgttttatca gaccgtgatt
     3061 ttatccacaa gttcaatgca agcttgtgaa taaattacgc acaaaatgtg acatagagat
     3121 gaaataccgg gaagagacaa cggggtctct ttccctgcta cggaacccat tgcagggaaa
     3181 gagtataaca cgcttttatt attcaggcgt aaagtggcgt aaatgttgta ccgtggcaag
     3241 ccacgctgcc acccagccaa tcatcgagca taccagcagc aatagcaggc attcatcgaa
     3301 cgataaacca ttgatatcaa acttcgttcc gaaaacctgt gccacttccg ccaccgctga
     3361 cgacaaacgc agtaccagaa tttctgacaa aattaatgac aacaatgcgc cagaaaatcc
     3421 cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa ccatccgtcg caccaatcag
     3481 tttctgcacg ttgatggagt cacggcgagc aaagatgctc agacgtacgc tgttgccgat
     3541 gacgaggaac actgccgcca ccatcaacac gccgatcatc gccgaaacgc gcccaaccag
     3601 cccggtcaac gccgccagac gggcaaacca gctgtcatcc atccgcactt cgtcaatacc
     3661 gttaatctgc gtgatacgat cacgcagcgt attgagtgat tccgtcccct gaaaatcgag
     3721 tttcgggatc accaccgcca ctgccggaag cgggttttct tccagcatat ccagcgcacc
     3781 accaaaacca gaccagttac ggaactcgcc cagtgcgtct tcacgagaaa gatagttcac
     3841 tttctccacg ccttgctcgg cctgcaactg tgccaccacg cccgcagcgg cgtcatcgtc
     3901 cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga taatactgcg tcgccgcctg
     3961 attaacgttt ttgtacacca tataacagac gctgggcagc gtcagggaga tggcgataac
     4021 catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga tcctgcaatg cgccgtggaa
     4081 ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga tttaccggtt ttggcgagga
     4141 tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag ccgccgaccg atttacggaa
     4201 gcgatccaga cgcccaccaa actgccgaat atgattgatt gcatcgcgct tattcatggc
     4261 ccacgcctcc atgcaagtga ccatcgctca gggtgagcat gcgataggaa cgccgtgaaa
     4321 tcagattaat gtcgtgcgtt gccatcaata cggttacccc aacgcggtta aactcttcaa
     4381 acagacgtag aatcccttcc gacaacgcgt cgtccaggtt accggtcggt tcgtccgcca
     4441 gcagtaccgc gggcttattc accaccgcgc gggcaatgcc aacacgctgt tgttcaccgc
     4501 cggaaagctg aatagggaag ttcttcgctt tgtccagtag cccgacttta tccagtgccg
     4561 ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc gataatcagc gggatcgcca
     4621 cgttatcgta gacagtacgg tccatcagta gatggtgatc ctggaaaatc atgccaatct
     4681 ggcggcgcag aaacggaact tcacggtttt tcagacgcgt gatgtcatgg ccgctaaacc
     4741 agattttccc ggcgctgggc cgctcaatcc cacagatcag cttcaggagg gtacttttcc
     4801 ctgcgccgga atgaccggtc agaaacgcca tctcacccgg ctgcatatgg aacgtaacgc
     4861 cctgcagcgc ctgtctccca ccgagataag ccttgctgac atgttcaaag cgaatcattg
     4921 ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc gccttaaacg gacgcaaatc
     4981 ctcaatacgt tcgccgacac caatgtagcg gatagggata ccaaactggt cagccaccga
     5041 gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt agcgtgatgc cggttaagcc
     5101 aacagcttcg tggaacagtt tggcctggct taccgcgttc tgcccggtgc tggcatcaat
     5161 agtcagcata acttcatgcg gcgcttcaac gtcgagtttc ttcatcacgc ggacgatttt
     5221 cttcaactct tccatcagat gcgatttgtt ctgcagacgt ccggcggtat cggcaatcag
     5281 gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg aagataacag aggcggaatc
     5341 cgcaccggta tgctgggcaa tcaccggaat attgttgcgc tgaccccaga cctgaagctg
     5401 ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc atcaccgatt taccctgctg
     5461 ctcaaactga cgcgccagct taccaatcgt cgtggtttta cccacaccgt tgacgcccac
     5521 catcaggatc acgaacggcg ttttatcttc aacattcagc ggctcatcga ctttcgccag
     5581 aatctcgccc atctcttctt tcagcaggcc atagagcgcc tcggcatcac gaagctgctt
     5641 gcgggatgcg ccttccgtca gattggtgat aattttacgt gtggtttcca cgcccacatc
     5701 ggcgatcaac agctgttctt ccagctcttc aaacagatca tcgtcgattt ttttaccgcg
     5761 gaacaggctg ataaatccgg aaccgagatt ttctttggtt tttaacaggc tgcgtttcag
     5821 gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc tgggcaattt ctgcaaccgg
     5881 ctgctcttct tctgccggag gaactaccat caccgcctct tctgccgctt cggcagccag
     5941 cgcctgtgct tccagctctt cgtcggtgat ttcttcttta gccgcttctt cttctgccgc
     6001 ttcgacaatc tctacggttt ccgcttcagc ctgccactct tctgacgaaa cctcttcggc
     6061 gttgacgtct tccggcaacg gcagctcttc acgttcgata gccactggct ccggcgtttc
     6121 ttctacgacc ggttccggct gtgcaacaac ttccgcttca ggctgcgctt tttcactttc
     6181 aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa gtttccgcct cggcttcagt
     6241 atgcgcctgc ggctgctctt caacggcttg ttcagaagcc ttcgcaggct cttgcgcctg
     6301 aacgatttct tctacaaccg gttgttcatt ctgaacttct gtctcttttt ccggggtctg
     6361 ctctttttga ccaaagccca gccaggaaaa aaagccacgt cttttttctt tcgccatttg
     6421 cgactacact cctcgctgtt gcttcatggc acagcgttaa cgctatgtac atagcagcta
     6481 aaaaaatgat gaaatagtct atcacttaac ttaattcaca tcaccgcctg caagtatgtg
     6541 ttatctggcg gattgagcaa tttatcatga aaaaaccgaa tcattccggc agcggccaaa
     6601 tccgcattat tggcgggcag tggcgaggcc gtaaactccc ggttcctgat agcccaggtc
     6661 tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa ctggctggct ccggtcattg
     6721 ttgacgccca atgtctggat tgcttcgccg ggagcggcgc gctggggctg gaagcgttat
     6781 cgcgctacgc tgcgggggca acgttgattg agatggatcg cgtggtttct cagcagttaa
     6841 ttaagaatct ggcgacacta aaagcaggca atgcacgcgt ggtgaacagc aacgcaatgt
     6901 cattcctggc gcaaaaaggc acaccgcata atatcgtgtt tgtcgatcca ccgttccgcc
     6961 gtggcttgtt agaagagacg ataaatttac tggaagataa cggctggctg gctgacgaag
     7021 ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc cactgttccg gcaaactggt
     7081 cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg gctgtatcaa cacgaagcac
     7141 aaggagaaag tgatgctgat taatattggt cgtttgttaa tgctctgcgt ttggggattt
     7201 ttaatcctca atctggtgca tcccttcccg cgcccgctaa atattttcgt taacgtggcg
     7261 ctgattttta ccgtgctgat gcatggtatg cagctggcgc tattgaaatc cactttaccg
     7321 aaagatggcc cgcagatgac caccgccgaa aaggtgcgga ttttcctttt cggcgtgttt
     7381 gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat aaccttattg ttcgccaaca
     7441 aagctaacaa agcgcgttcc tttatagctc agcgtacctt tatcgcccac tgtcagggcg
     7501 tggtactgct gggcgtcgag gcgaaacgtc tgctccattc ctccgctttg cggtttgaag
     7561 cttgcctcat agcgcatact ggtgcctgcc ggagtcactt cctgctggcg cgaacggcga
     7621 tcgttaatcg gtttttcccg cttgttgctc accaccacca gcttttgctg gagcggagcc
     7681 atatcattat cagctttttc ccgtcgctgt tgcataaaac gaaacgatgc ggcgacgaca
     7741 attaagccaa tgataacaat aaagaaaaga ggtggtttac tcatctttat ccctcatcgg
     7801 aaaatgcgga attaagcata ccctgccagt tatggtgttg tcatccgtcc accctcgcca
     7861 ctaaactgga agcaagaccg taggcattct gcttacgaaa aaataacgaa ttcaaggaac
     7921 taagatgctt tggtcgttta tcgctgtctg tctttccgca tggctatctg tggatgcatc
     7981 gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg ttaacccttc ttctcctgct
     8041 gttactggcc tggcaagcac cgatgttcga cgccattagc tatctggtgc tggcgggact
     8101 gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca cgtcaacgtc tgatgtacgc
     8161 catcggcgcg tttttcctct cgcacctgct gtacaccatc tatttcgcca gtcagatgac
     8221 gctctctttc ttctggcctc tgccactggt gttgctggtt ctgggtgcgc tgctactggc
     8281 gattatctgg acgcggctgg aagagtaccg ttggcctatc tgcacgttta tcggcatgac
     8341 gctggtgatg gtgtggctgg caggtgaact gtggttcttc cgtccgaccg ctccggcgct
     8401 ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac tttgtctggc tggggagcca
     8461 ctatcgccga cgcttccgtg cggataacgc gattgctgcg gcctgctact ttgccggtca
     8521 cttcctcatc gtccgctcac tgtatctctg ataaaacttg actctggagt cgactccaga
     8581 gtgtatcctt cggttaatga gaaaaaactt aaccggagga tgccatgtcg acacctgaca
     8641 atcacggcaa gaaagcccct caatttgctg cgttcaaacc gctaaccacg gtacagaacg
     8701 ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc aacgctctct gaaaacgtct
     8761 ccggcacccg ctatagctgg aaagtcagcg gcatggactg cgccgcctgt gcgcgcaagg
     8821 tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt gcaggtgttg ttcgccaccg
     8881 aaaaactggt ggttgatgcc gacaatgaca tccgtgccca ggttgaatct gcggtgcaaa
     8941 aagcgggcta ttccctgcgc gatgagcagg ccgccgacga gccacaagca tcgcgcctga
     9001 aagagaatct gccgctgatt acgcttatcg tgatgatggc aatcagctgg ggtctggagc
     9061 agttcaatca tccgttcggg caactggcgt ttatcgcgac cacgctagtt gggctgtacc
     9121 cgattgctcg tcaggcatta cggctgatca aatccggcag ctacttcgcc attgaaacct
     9181 taatgagcgt agccgctatt ggtgcactgt ttattggcgc aacggctgaa gctgcgatgg
     9241 tgttgctgct gtttttgatt ggtgaacgac tggaaggctg ggccgccagc cgcgcgcgtc
     9301 agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc cacgcgcctg cgtaacggtg
     9361 agcgggaaga ggtggcgatt aacagcctgc ggcctggcga tgtgattgaa gtcgccgcag
     9421 gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt tgccagtttt gatgaaagcg
     9481 ccctaaccgg agaatccatt ccggtggagc gcgcgacggg cgataaagtt cctgcaggag
     9541 ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc agaaccgggt gccagcgcca
     9601 ttgaccggat tctgaaactg atcgaagaag ccgaagagcg ccgcgccccc attgagcggt
     9661 ttatcgaccg tttcagccgt atttatacgc cagcgattat ggccgtcgcc ctgctggtaa
     9721 cgctagtgcc gccgctgctg tttgccgcca gctggcagga gtggatttat aaaggtctga
     9781 cgctgctgct gattggttgc ccgtgtgcgt tagttatctc aacgcctgcg gcgataacct
     9841 ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa aggcggtgcg gcgctggaac
     9901 aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg tacgctgacc gtcggtaaac
     9961 cgcgcgttac cgcgattcat ccggcaacgg gtattagtga atctgaactg ctgacactgg
    10021 cggcggcggt cgagcaaggc gcgacgcatc cactggcgca agccatcgta cgcgaagcac
    10081 aggttgctga actcgccatt cccaccgccg aatcacagcg ggcactggtc gggtctggca
    10141 ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc tgccgggaaa catcccgctg
    10201 atgcatttgc tggtttgatt aatgaactgg aaagcgccgg gcaaacggtt gtgctggtag
    10261 tacgtaatga tgacgtgctg ggtatcattg cattgcagga taccctgcgc gccgacgcta
    10321 caactgccat cagtgaactg aacgcgctgg gggttaaagg agtgatcctc accggcgata
    10381 atccacgcgc agcggcggca attgccgggg agctggggct ggagtttaaa gcgggcctat
    10441 tgccggaaga taaagtcaaa gcggtgacca agctgaatca acatgcgccc ctggcgatgg
    10501 tcggtgatgg tattaacgac gcgccagcga tgaaagctgc cgccatcggg attgctatgg
    10561 gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc attaacccat aaccacctgc
    10621 gcggcctggt gcaaatgatt gaactggcac gcgccactca cgccaatatc cgccagaaca
    10681 tcaccattgc gctgggactg aaagggatct tcctcgtcac cacgctttta gggatgaccg
    10741 ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt gctggtgacg gcgaatgcgt
    10801 taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac attgagcgcg atcggtcccc
    10861 tcgcccctct ggggagaggg ttagggggtg aggaaaaggc ggcatcgaag ccaatcagcc
    10921 cctatcaacc gcctttacga atcaaataac gataaggcag tccatccgtc tctttcgcaa
    10981 ccagttcgtg ttccataaag gtacaaaacc caggaatatc gcgggtagtg gccggatcgt
    11041 cggcgataat cagcaacgtt tcgccaggct gcatattgcg cacggttttg cgcaccatca
    11101 tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt gtggtcaggg ctggaaaaga
    11161 gatcggtcat tttcttctca tcacttaaaa aaacggcgct agtttacgcc ctgtgagtcc
    11221 gtaagcaaac caggttaacg attgcgtgaa aattaaccat tgcattgtca acgtaaagca
    11281 gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt atgttaaggt aacagacgtg
    11341 tcgtacgtat tgggttccct caccccaatg gttaatcaaa aaggtacaat atgaacgttt
    11401 tctcgcaaac tcaacgctat aaggcgttgt tctggttatc gttatttcat cttctggtga
    11461 tcacctccag taactatctg gttcagcttc ccgtctccat ttttggtttc cataccacct
    11521 ggggcgcgtt tagctttccg tttatttttc ttgctaccga cctgaccgtg cgtatttttg
    11581 gcgcaccgct ggcccgacgc attatcttcg cggtaatgat ccctgcgtta ttaatctcct
    11641 acgtcatctc gtcgctattc tatatgggtt cctggcaggg attcggcgca ctcgcccact
    11701 tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat ggcctacgcg ctggggcaaa
    11761 tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg ccgctggtgg ctggcaccga
    11821 cagcgtccac actgttcggt aacgtcagcg acacgctggc ctttttcttc attgccttct
    11881 ggcgtagccc ggatgccttt atggctgaac actggatgga aatcgcgctg gtcgattact
    11941 gtttcaaagt gttaatcagt atcgttttct tcctgccaat gtatggcgta ttactcaata
    12001 tgctgttgaa aagactggca gataaatccg aaatcaacgc tttgcaggcg agttaaaggt
    12061 tcgttatcag agttgtgata agatggatga atgagccgtt atggccgttt atcgaaagga
    12121 agaagtcaat gcgcaatctg gttaaatatg tcggaattgg cctgctggtt atggggcttg
    12181 cggcctgtga tgataaagac actaacgcta cggcgcaggg ttcggttgcg gaaagtaacg
    12241 ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag tttctcgctg ccagcggata
    12301 tgaccgacca gagcggtaag ctgggaacgc aggccaataa catgcatgtc tggtccgacg
    12361 ccaccgggca gaaagcagtc atcgtcatca tgggcgatga tccgaaagaa gatctggcgg
    12421 tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga tccgcagctg caagtggtaa
    12481 ccaataaagc cattgagctg aaaggtcaca aaatgcagca gttagacagt attatctccg
    12541 cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa cgtgggtaat caactgctga
    12601 ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc gcaaaccacc gcagaaaaca
    12661 tcattaatac gctggttatt cagtaagttt taagatgatg aggcggcctc agggacgtgt
    12721 tccggaggcc gtttttttaa tcgccacgtc agtaataacg cgattgcgac cagtcccgcc
    12781 gccgccagat aaatcaccgg tacgcccgcc cagctcatca ccagcccagc cagtggtcca
    12841 gtcacgccaa gcgataaatc cataaatacg gtgtaagttg ccagcgccgc cccctgattt
    12901 tgctgcggaa ccgcttttac cgccactaca cccaatgccg ggaacaccag cgaaaacccg
    12961 gcccccgcca gtaagacgcc gattttcgcc atccacggca tagtcgccac gccaaccagt
    13021 agcaggccga ttatctcaac gctaaagcaa atcatcgcta cgtttaagcc accgatacgg
    13081 ttaatgccgt tagggaataa caaacgcgta ccgacaaacg cacagctaaa cagcgtcagc
    13141 gcgaaagccg caccgtccca acctttagcg tcataaaaca gcgtgataaa ggtggcgatg
    13201 acgccaaatc cggcggaagc cagtgccagc gccataccgt acagccagac gcgcccaagc
    13261 accgcgcgaa acggcagcgg tttgccttta ctggctttta ccgtcggacg cgggatcgcc
    13321 aacaaaatgg ccaccagcgc cacgcccata atgattaacg ctaacgcctg caagccgccc
    13381 cagtgataaa acacgacgcc taacggcgca cccatcgcca tcgccccgta agtgacaatg
    13441 ccgttccacg aaatcacccg cccgatatgc agcgagccaa ccactccaac gccccatagg
    13501 gtcgatcccg ttccggcaaa actttgccca atcccaagga tgacgcgccc caggcaaagt
    13561 aataacaggc tgatgacagg cagactggcg gttaatcctg ccgtcagata ccccagaccg
    13621 ctcaaaaagc agccgcataa accgaagacg acaatctttt tgggtcccag caaatcggcg
    13681 taacgtccgg catgagggcg gctcagcaag gtggcgaaat attgcaggct gataaccaat
    13741 cctgcccaga aggcgctaaa gcccatcaca tcatggacat agcccggtaa tacagcgagc
    13801 ggcaacccga tggtgaggta gctggcgaag ttaaacatga caatggagac aatgcgcaaa
    13861 ttcaggcgca atccgtttag cgcgggttcg gctacgggtt cgggcatgag gatcaccaca
    13921 tttttacaac agtgtttcat ttttaccatg tgctgacgtg aaaatcagca gtaagaatca
    13981 gaatattgct ggcgtgactc ccgcgctaca cttaacacaa aaagtcacaa ggaagcccca
    14041 atggaaaccc ctcaacccga taaaacgggc atgcacattc tgctcaagtt ggcctcgctg
    14101 gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca ttgtgcagct gttactggcg
    14161 ctgttttttg ccatcgtcct caacccgctc gttacctggt ttattcgtcg gggagtacaa
    14221 cgccccgttg ccattacgat tgtagtggtg gtgatgctga tcgcactaac cgcgctggtc
    14281 ggcgtactgg cggcatcgtt taacgaattt atctctatgc tgccgaagtt taataaggag
    14341 ctgacgcgca aactttttaa attgcaggag atgttgcctt ttcttaattt gcatatgtcg
    14401 ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg ttaccttcac cacagcgcta
    14461 atgaccgggc tttccggggc aatggcgagc gtgcttttgc tggtgatgac cgtagttttt
    14521 atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt ttgcgctgaa taacccacag
    14581 attcacatcg caggattaca ccgcgcactt aaaggcgttt cgcattatct ggcgttaaag
    14641 acgctactca gtttatggac aggtgtcatc gtctggctgg ggctggcgct aatgggcgta
    14701 cagtttgcgc tgatgtgggc agtactggcg tttttgctca actacgtgcc caatatcggc
    14761 gcggtaattt ccgccgtacc gccaatgatt caggtgctgc tgtttaatgg tgtttacgaa
    14821 tgtattctgg tcggcgcatt gtttttagtg gtccatatgg tcatcggcaa tattttagaa
    14881 ccacggatga tgggccatcg cctggggatg tccaccatgg tggtatttct ttcattgtta
    14941 atctggggat ggctgctcgg cccggtaggg atgctacttt cggtgccatt aaccagcgtg
    15001 tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac tggcgatttt actggggccg
    15061 ggcagaccga aaagtcggtt accgggatga ggcgacaagt gatacgatac gcactttcat
    15121 tttccattaa acgttggccc tgatatgtat cggatagttc tggggaaagt ttcgacctta
    15181 agcgcagctc cactgccacc gggtttacgc gagcaagcac cgcaaggtcc acgacgcgaa
    15241 cgctggctgg cggggcgtac attgctttcg cacacgcttt ccccgctacc ggagatcatc
    15301 tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc cgctatggtt caacttaagc
    15361 catagcggtg acgatatcgc tctgctgttg agcgatgaag gcgaagtcgg ctgcgatatc
    15421 gaagtgattc gcccgcgcgt caactggcgc tggctggcga acgccgtgtt cagcctcggg
    15481 gaacacgctg aaatggacgc cgtgcatcct gatcagcaac tggaaatgtt ctggcgcatc
    15541 tggacgcgca aagaagccat cgttaaacag cgtggcggca gcgcctggca aatcgtcagc
    15601 gtagatagca cctatcactc ctcgctgtca gtcagccatt gtcagctaga aaatttaagc
    15661 cttgcgatct gcacccccac cccctttacg ctcaccgccg acagtgtgca atggatcgat
    15721 tcagttaact gatccgccca cccgactgcc catctattga tccagaacag gtaatcagta
    15781 tgacgaatac ttaaaatcgt catacttatt tccgccatct attttaatcc attggggtta
    15841 ccatgctctc cacactccgc cgcactctat ttgcgctgct ggcttgtgcg tcttttatcg
    15901 tccatgccgc tgcaccagat gaaatcacca ccgcctggcc ggtgaatgtc gggccactaa
    15961 acccgcacct ttacacgcct aaccagatgt tcgcccagag catggtttat gaaccattgg
    16021 tgaaatatca ggcagacggt tcggtgatcc cgtggctggc aaaaagctgg actcattcag
    16081 aagatggtaa aacctggacc ttcaccctgc gtgatgacgt gaaattctcc aacggtgaac
    16141 cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt gctcgataac cgtcaacgtc
    16201 acgcctggct ggagctggca aaccagattg ttgatgttaa agcactcaat aaaacagagc
    16261 tgcaaattac cctgaaaagc gcctactatc ctttcctgca agaactggcc ctgccccgtc
    16321 ctttccgttt tatcgctccc tcgcagttta aaaaccatga aaccatgaac ggaattaaag
    16381 cgccgattgg caccggaccg tggattttgc aggaatcgaa actaaatcag tacgatgtct
    16441 tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat taaaaagatc acctttaacg
    16501 tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac tggcgatatc gacctgctgt
    16561 acggaaacga agggttatta ccgctcgata ccttcgcccg ctttagccag aatccggctt
    16621 accacaccca actgtcacag ccgatcgaaa ccgtgatgct ggcgctcaat accgccaaag
    16681 cccccaccaa cgagctggca gtacgtgaag ctcttaatta cgcggtaaac aaaaaatcgc
    16741 tgattgataa cgcgttatat ggcacccagc aggtcgccga caccctgttt gccccttctg
    16801 tgccttacgc taatctcggc ctgaaaccgc gccagtacga tccgcagaaa gcgaaagagt
    16861 tgctggaaaa agccggttgg acgctgcctg cgggcaaaga catccgcgag aaaaatggtc
    16921 agccgctgcg cattgaactg tcgttcatcg gcaccgatgc gttaagcaaa tcgatggcgg
    16981 aaatcattca ggctgatatg cgccagattg gcgcagatgt ctcgctgatt ggcgaagaag
    17041 agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg catgattttc caccgcacct
    17101 ggggcgcgcc atacgatcca cacgccttcc tgagttcaat gcgcgtaccg tcgcacgctg
    17161 acttccaggc acagcaagga ttagccgaca agccgctgat tgataaagag atcggcgaag
    17221 tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta tcgcgacatt ctgacccgtc
    17281 tgcatgacga ggcggtttat ctgcctatca gctacatctc gatgatggtg gtatcaaaac
    17341 cggagctggg taatatcccc tacgcgccga tcgccaccga aattccgttc gaacagatta
    17401 aaccggtgaa accttaatgt tgcgttacgt attacgccgc tttctgctgc tgatcccgat
    17461 ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc ctcggtaccg gcgacccggc
    17521 gctcgattat ctgcgtttat ctaacctgcc gccgacgccg gagatgctgg cctctacccg
    17581 caccatgctc gggctggatc agccgctgta cgtccagtac ggtacctggt tgtggaaggc
    17641 gctgcatctt gactttggta tctcattcgc cagccaacgc ccggtactgg acgatatgct
    17701 gaacttcctg cccgccacgc tggaacttgc aggtgcggcg ctggtattaa ttctgctcac
    17761 ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat cgtttgccgg atttcgccgt
    17821 gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc tggctggcat ttttgctagt
    17881 gatggcgttt tcggtgtatc tgcaatggct acccgcgatg ggctacggca gctggcagca
    17941 catcatttta cctgcggttt ccatcgcctt tatgtcgctg gcgattaacg cgcgactgct
    18001 gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc acctgggcgc ggctacgcgg
    18061 cctgaatgac aaacagaccg aacgccgcca tattctgcgc aatgcctcgc tgccgatgat
    18121 caccgccgtg gggatgcaca ttggcgaatt gattggcggg acgatgatta tcgaaaacat
    18181 ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg atttttaacc gtgactatcc
    18241 ggtgattcag tgctttacgc tgatgatggt ggtggttttt gtggtctgta atttgattgt
    18301 cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt catgaaggag cgcacgcgtg
    18361 aactttttcc tctcttcccg ctggtcggta cgcctggcgc tgatcattat cgccctgctg
    18421 gcgctgattg cgctcaccag ccagtggtgg cagccgtacg atccacaggc aattgatttg
    18481 ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg gcaccgatca cttaggtcgc
    18541 gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc tcggttcggt aatggcctgc
    18601 ctgctgctgg tgctgacatt agggctggtt attggcggca gcgccgggtt gattggcggg
    18661 cacgttgatc aggccaccat gcgcgtcgcc gatatgttta tgaccttccc gacctcgatt
    18721 ctgtcgttct ttatggttgg cgtgctcggc accgggctga ccaacgtaat tatcgccatc
    18781 gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca gcttggtgat ttcactacgc
    18841 caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg gccatgtgcg ggtgtttgtc
    18901 gatcatctgg caggcgcggt gatcccttcg ctgctggtgc tggcaacgct ggatatcggc
    18961 catatgatgc tgcacgtcgc ggggatgtct ttccttggcc tcggtgtgac cgcgccgacc
    19021 gccgaatggg gcgtgatgat taacgacgcg cgccagtata tctggaccca gccgctgcaa
    19081 atgttctggc cggggctggc gctgtttatc agcgtgatgg cctttaacct ggtgggtgac
    19141 gcactgcgcg atcatctgga tcctcatctg gtgacggagc acgcacacta atgccgcaac
    19201 agattgaact acgtaatatc acgctacagg ccgcgcagcc gctggtacac ggtgtatcgt
    19261 taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg tagcggcagc gggaaatcat
    19321 taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt tcgccagacg gcgggggaaa
    19381 ttttagccga tggcaaaccg gtttcgcctt acgccctgcg cggcatcaaa attgccacca
    19441 tcatgcagaa cccgcgcagc gcctttaatc cactgcacac catgcacacc cacgcgcggg
    19501 aaacctgcct ggcgttaggg aaacccgccg atgacgctac gcttaccgct gccatagaag
    19561 cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta cccgttcgag atgagcggcg
    19621 gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg tgaatcaccg tttatcatcg
    19681 ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc gcgcatcctc gatctgctgg
    19741 aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt cacccatgat atgggcgttg
    19801 tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg taaaattgtc gaacagggcg
    19861 atgtagaaac gctgtttaac gcccccaaac atacggtgac gcgcagcctg gtttccgctc
    19921 atctcgccct ctacggtatg gagctggcat catgacctta cttaacgtct gcggcctttc
    19981 ccatcactat gcccacggtg gatttagcgg aaaacatcaa catcaggcgg tgctgaataa
    20041 cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg ctgggacgca gcggctgcgg
    20101 aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct ccctcacaag ggaatattag
    20161 ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa cgtaaagcct tccgccgtga
    20221 tattcagatg gtgtttcagg actccatcag tgccgtgaat ccgcgcaaaa ccgtgcgcga
    20281 gatcctgcgt gaaccgatgc gccacctgct atcactgaaa aaatccgaac aactggcgcg
    20341 cgccagcgaa atgctgaagg cggtcgatct cgatgacagc gttctcgaca aacgcccacc
    20401 gcagttaagc ggcggccagc tccagctcgt ctgcctggca cgcgcgctgg cggtcgaacc
    20461 gaaattgctg attctggatg aagccgtttc taaccttgat ctcgtgttac aggcgggcgt
    20521 cattcgcctg ctgaaaaagc tacaacaaca gtttggcact gcctgcctgt tcatcactca
    20581 tgacttacgc ctggtggaac gcttttgcca gcgagtgatg gtgatggaca acggacaaat
    20641 cgccgaaacc caggcggtgg gagataaatt aaccttttcc tctgacgccg gacgtgtgct
    20701 acaaaacgcg gtattacccg cattccccgt gcgccgtcgc acctcagaaa aggtttaacg
    20761 ccaatgcaac gagtcaccat cacgcttgat gacgatttac tggagacgct ggacagcctg
    20821 agccagcgtc gtggttataa caaccgttcc gaagctatcc gcgatattct gcgtagtgcc
    20881 ctggcgcagg aagctaccca gcagcacggc acacaaggtt tcgcggtgct gtcgtatgtg
    20941 tatgaacacg aaaaacgcga cttagccagc cgtattgttt ccacccagca tcatcaccac
    21001 gacctctccg tcgccacgct acatgtgcac atcaaccacg acgactgtct ggaaatcgcc
    21061 gtgttgaaag gtgacatggg tgacgtgcag cattttgccg atgacgttat cgcccagcgc
    21121 ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag attgagttat ttctaaaaca
    21181 aggaaagaaa agatattaac tgaacttaat ttataaaatt aagttcagtt gcatagatag
    21241 atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta aaatttataa atgattcaaa
    21301 cgctcattca gtgtttcaat aatataggta ttaacagaga cttgttgctg cgcagccgca
    21361 ttattaaggc gttcactcag cgattctgga tagcgtaacg taaaggtctt aatcttttct
    21421 gtacgcgcat acggctcaat acccgcagct ttacaatcct caagatactc tcgcagggaa
    21481 agctctcctt ctttttgcag cccctgaata ctgtcagaga caaaatcaca ataacctgac
    21541 aagcccagga atttgccacg aaatgcattc agttctggca cgtaggtaat aacagctggt
    21601 tgcccggcaa tctccataga gtttggcgtc ttcagtttga tcatggtttt acccctatac
    21661 ttagtaacca gtcacacacg ctttctacag cgcctttatc ggtatctggc gacggatgag
    21721 gccgatggaa acacgcaacg ctcatattta gtatgaactt gcaacgcgaa cctcttcctt
    21781 ccttaatttc tccgcctaat gctttaacca gtgactctat atcagaccac ttgatccctt
    21841 gaggaacagg agttttaaat atttgatcca atgtattttt ttgtttcttc cgtaaagcct
    21901 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcac agtatgaagt
    21961 caagtttccc ttccttacgc cattgtccca atcgtcttcc tgaatcgcag cagtgcgatg
    22021 gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact gcggccagac gatttcgaat
    22081 ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa agtgtgtcgt cggcatggtc
    22141 agcataatgt cctgcaccat ctgcggcata ctttcgcgcg gcgtggatcc accggaaagc
    22201 atttgcagcg gcagcagcac cagaatcacc agcagcccca gttgcggcat tgaacgcgct
    22261 atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca gactgagcgc cacgcccagc
    22321 ataaacagcg ggatcgagcc ttcaatcggt acgcccagta cacctttcac catcagcacc
    22381 agcgataatc ccgataccac cagcaccacc agccccatcg accagatctt cgccatcatg
    22441 atctcaaacg gcgttatcgg catcaccagt aaatgttcca ccgtgccgtg ttcacgctcg
    22501 cggatcagcg ccgatccggt caatacaatc gccagcatgg taatgttgtt gatgatcgcc
    22561 atcaccccgc caaaccacgc gggatcaagg ttcgggttaa agcgcatccg ggtttccagc
    22621 gataccaacg gttcgctgtt atcacggtag cgcgcgacaa agctgttcac ttcaccgttg
    22681 ataatattct ggatataccc attgccggta aatgcctggc tcatgcgcgt ggcatcgacg
    22741 ttcacctgaa tatccggctg gcgtccggcg aggacatcac gctgaaaatt aggcggaata
    22801 tttatcgcga aggtatagcg tccggcgtcc agtccggcat ccatctcatc ggcggtgatc
    22861 atctccggtg gcaaaaacca cggacgatag aagctgttaa cgatccggtt cgataactgc
    22921 gattgatcca tatcggcaat ggcgatcggc gcgaggttca acgatcctgg cgtaacggtc
    22981 gctgacgaat acaccgacac cgtaaacgag aagacaatca gcgtcagcat cgctttatca
    23041 ccgagcagac tgcgcaactc tttgataccc agattaaaaa tattgcgtaa atggcgcatc
    23101 atccctcctg ttttttcagc agcaggatgc ttaagcccat caccagcggg atggctatca
    23161 gcaacgggat aaaaagttgc cacaaatcag tcagatccag cgctttcgag aacgttccgc
    23221 gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc gatccaacgt ccaggccctt
    23281 ccagcgaagc taccggatcg atcatcccgg aaaactgtgt cgccgggatc aatgtgataa
    23341 tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa ggtggagatc agcagcccca
    23401 ttccagtggc aatgatgata tacagcagcg ccgccaaggt gagcgtcagg aaactgcctt
    23461 tatgcggtac gccaaacaca aacaccgaca ggccgcagag caggaaaaag ttcagcatcc
    23521 ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc gctacgggtg gtgggggtca
    23581 cgtaaaggtt gataatcgac cctagctctt tttcccgcac cacgctaagg gcgcttagca
    23641 ttgacgggat catcatcagc agaagcggga tcaccgccgg aacaatcgct ggcaggcttt
    23701 ttacgtccgg gttatagcga taacgcgtct caatgttcat cagcccgctt tggctggcgg
    23761 gtgtcgattg tcggctcgcc acatcctgaa gccaactctg gtgcattgcc tgcacgtaac
    23821 cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat ccagacgccg agttccacag
    23881 gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat ctcaatggcc accgtgatat
    23941 cgcccgcacg catccgacga tcaagctcgt cataactggt gagcggcggc tgttcgataa
    24001 agtaacggga accggagagg ttgagtgtcc acgcctggct actgacggtc tggtcgcggt
    24061 cgagcaccgc aaagcgcagg ttttccacat ccatactgat gccgtaaccc attatcagca
    24121 tcaggatcac cgttcccatc agcgccagcg tcgaacgtac tggatcgcgt cgcagttcca
    24181 gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct aaatccctga cgcggcgcgt
    24241 gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt gctctgccct gccgcttcct
    24301 gcaaataggc gataaatgcc tcttccagac tggcggctcc gcgtttctca accagttcct
    24361 gcggtgtacc gctggcaagc acttttccgg cgtgcatcag tgagatgcgg tcgcaacgtt
    24421 ccgcttcgtt cataaagtgg gtggagatga agatagtcac tttgtcctgg cgcgagagat
    24481 cgaccatcaa ctgccagaac atatccctcg ccaccggatc gacaccagaa gtaggctcat
    24541 cgaggattaa catctccggg cgatgaatca ccgccaccgc cagcgaaagc cgctggcgaa
    24601 tgccgagcgg caatgactcc ggcagaacat cttcaacgtc gttgagctta aaacgctcgc
    24661 tcatttcagc cactcgcgcg ggaatttccg cttccgggat gtgaaacaaa cgggcatgta
    24721 actccaggtt ttgccgcacg gtgagttcgt tatagagcga aaacgcctgc gacatatagc
    24781 ccacacgacg gcgggtatcg atatcttttg gatcaaccgg ttgcccaaat agccacgcct
    24841 caccttcgct ggcgggcagc agtccggtaa gcattttcat ggtggtggat ttaccgcagc
    24901 cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat gcggaaattg acgtgatcaa
    24961 cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc ttcgatggca atctctgcgt
    25021 tttcaggttg atacggtggg atcactaccg cctgatgcgc ctggcgttgc gcttgcggta
    25081 acagattaat aaatgcttct tccagcgtgg cgctttgcgt ttgctgccgc agctcttcgg
    25141 cgctgccggt tgccagcact tctccggcat tcatcgctac cagccagtcg aagcgttcgg
    25201 cctcttccat ataggcggtg gcgaccagca cgctcatatt gctctgccgc tggcgaatac
    25261 tgtcgatcag atcccagaac tgggcgcggg agagcgggtc aacccccgtt gttggctcat
    25321 caaggatcaa cagttccggg tcgtggatta acgcgcagca cagcccaagt ttttgcttca
    25381 tcccgccgga gagtttacct gccggacgat cgcgaaacgg tgctaacccg gtgctggtca
    25441 gcagctcatt aattcgcgct tcccgctccg ctttatcgtg accgaacagg cgagcgaaaa
    25501 aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt tttgcccagc ccctgcggca
    25561 tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg gtcgtgcata tcgccaccca
    25621 gcaccaccac attgccctgc tcaatgacgc gggcaccgga aatcagtgac aataaactcg
    25681 acttcccgac gccatccgga ccaatcagcc cgaccatgca acgggccgga atatcgagag
    25741 tgatattgtt cagcgcaacg gtttttccat aatgctggct cacgcccgcc agttgcgcga
    25801 caggcgggac gggaaccagt tccagatgcg tcattgcggc aacctcacca cgaggtcgtc
    25861 aggccacgga agttcttcat tcacccgcac ccacgctacg cccggtaaac cggttttgac
    25921 atattccaga tgctgctgga gtaattccgg tgggatacgc gctttgacgc ggaacatcag
    25981 tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg aactgggcga cactggcgac
    26041 aaaactgatg gttgcaggaa tacgcagatc tggcgcggca tcgaggatca gccgggcttc
    26101 accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg aagaaggtca tatagacgtc
    26161 gctgagatcg accatattca gcacccgacc gcctgccgcc agcacttccc ctggctcggc
    26221 aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt tcactgtcat cgatatctgc
    26281 ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga gtttgcgcct gaatgatatt
    26341 ggtgcgtgct gcttctatag ccgctttaga agccgaaacc tgggctttcg ctgattccag
    26401 cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc agctgttgcg cagaaatagc
    26461 ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg gcaacagagt ccagttcggc
    26521 ctggcgttga ttgaccagcg actgtgcggc acgagtttca ctttgtcgtt gctccagcaa
    26581 agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt tgcgcgatgg cttccagtcg
    26641 ctgttcctgc aacacgcgag tatccatctt cgccagcact tcgccttcgc gaacaaactg
    26701 gccttctttc accagaatgg tgtcgatacg cccggcaatt ttgctggcga tatccacttc
    26761 cgtcgcttca atgcgcccat tgctgacagc aaagccttcc ggcacacccg ccgggcgcaa
    26821 cagccaccac gccacgacgg cagccaccgc cagtgccccg acaacccacc acgccagatg
    26881 gcgcttactc ttatccataa tcgacccgcc ataatccctg taagcaaacg acgcgtgtta
    26941 caactgccgc cgaacgacgt tcagcggatt cttccatccg ggataagaag gaaacgacaa
    27001 atacgaccag cgcgcagagc attgagcgcc aacggggaag gcttagtgaa aacgggtagt
    27061 aatgttgtca tcatcagcat tcctggccgt aaatgaaaca cgcatactca taagtgtgat
    27121 ctataaaact ggctgacaaa acggtatcca tcctgttgga tcgcatttca cgcctgttca
    27181 gttgcaggaa cagagcattc ctttttacgt tttatcaaca gtctcaacca gatagtaaca
    27241 ttaatattca tgttgtctat ggttcagagc tgtaacattg tgcgccttct aatcaaaggc
    27301 ataagtccat ttctgttttt ttcatgctgg taagacttcc agggagtggt tatctctggc
    27361 cctgcaccag ggttaatttg gcaattatgc atgctgaaca ttcttccagc atgtttactg
    27421 tcacgggcgt taagacctaa aactgtcccc gtttcaccta tgcgttctct cccaccattt
    27481 ccgttatgtg caacaacttg atttatacct aacatttgtg aacatgctaa tagtgcttca
    27541 agtttaggtc tgtaatctgt cgaaacagaa aaataacgat taaaggatcg acaagagata
    27601 gcccggctag ttttaaactt tttgccatta attatagcat gatgcttttt attaataatt
    27661 tccgctaatt cgaccggatc gagagcattg gtcatttcat caatagagac agttatcggt
    27721 cctataatgt aacgttttcg ctgatcatca aaagttatcc catgatgatt tgccattatt
    27781 ttgtatttgg ggtcatagaa acatacagga taatctttta tcccgtcgta ggtatcctca
    27841 tcaagtggtt tcatgcgcgt gaagttttgc atatagttac cattgcaatt cgtttcatga
    27901 tttccagcta atacatgaac attttcattg atactcatta tgcgttttaa caaatcgatg
    27961 ataaattgat cgccacagat attactaaat cgatcgccgg tttggtcgcc aagaaacgta
    28021 catggtgtga ctgcgtcttt attaataacc aaataaggct gaagactttc taaaacatta
    28081 tctcttctgt tcagtatact gattattgta ttaacattag tgtcaagttg ctcattaaag
    28141 ttctccctta gcaatacgtt gagaacttca tcttctatat taagcaactg gctaaggaaa
    28201 ttttctcctt cagccatgat tcccaaatgt cctgttgcaa aaagcgcgta caaaactgca
    28261 ccgactgagc catcggtatc gccaaaatag gtcggcctgg tacttgtggg atcaacaggt
    28321 gaacaattgg agaaataggt tctgtcagcg aactggggtt ttataattgt ttcgaattta
    28381 atatcaaata cattgtattg cgatatttcc cagttccaga tgggtttgta cgactcagta
    28441 actgcaacat cattagcatt atcatttact gtcatattat taagtaaata aacaatgctg
    28501 caccaattaa tatcggtatt atcaagaggg caaccgtaat ttttactatt aattgtcaca
    28561 ataaactgat aacagttatt cctacgtaga atatgagcgc gcatgcccga tagcatacta
    28621 tatttttctg aatgattcgt ttgtatggta aatgaaattt tgtcatcttg aatagattgt
    28681 tggaaacgtt ctatttgcca atcatcaatt ttttccacaa cttcaccgag cattgtcctg
    28741 gcaatacttg atatatcatc acctatacca tttgcaatat acccaaacag ttctttagtg
    28801 aagttggtaa catgctcttg caccatatca tttgttgcga ttgtcgttgt actgccatta
    28861 gtgcctgcca cagttccaat tttcatcttt tacctcatat tattcaccgc gaggaataca
    28921 tagttaacat tttatcttat gacatacacc tcatattcga tggggaaata attatatttt
    28981 gcacaaatca ttcagcctta aagcataata tgaaatttct tatctcatag cagagaaagt
    29041 ccctaaatat tgtagaggcg tcatcagtac atacctctac aatcgattaa gaaaacctat
    29101 gaaatactgg caaaattaaa aagagatacc acagcactca tgaagtttta ttgaggttga
    29161 aaacaactaa gccctgggac acctttttta tcaccacgtt cgttaggtgc tatcttccaa
    29221 gaatttagcc ccatggtata attatcaatt ttttgatgct ggtctacccc taatccataa
    29281 tggccaaatt tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt
    29341 tttggtctga aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata
    29401 gcattgcttc taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta
    29461 catatatttg ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc
    29521 aaagcaccga gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt
    29581 aaaactttag tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta
    29641 tcaccggcag agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt
    29701 tcatgatttc cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc
    29761 atatttcgca tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat
    29821 ctatcgcctg tatgatcccc aaggaagata caaggggtta tcgcatcgtc ctttaaaaaa
    29881 acatgttgat gcaactcatt caatatcatg tctctcgtat
//
LOCUS       DBBBSB010000042        29578 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.042, whole genome shotgun sequence.
ACCESSION   DBBBSB010000042 DBBBSB010000000
VERSION     DBBBSB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29578
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.042"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..215)
                     /locus_tag="ACIUS0_001837"
     CDS             complement(<1..215)
                     /locus_tag="ACIUS0_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HGB0219606.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
     gene            525..2297
                     /gene="aspS"
                     /locus_tag="ACIUS0_001838"
     CDS             525..2297
                     /gene="aspS"
                     /locus_tag="ACIUS0_001838"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGB0219607.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2415..2867
                     /gene="nudB"
                     /locus_tag="ACIUS0_001839"
     CDS             2415..2867
                     /gene="nudB"
                     /locus_tag="ACIUS0_001839"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGB0219608.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2896..3636
                     /locus_tag="ACIUS0_001840"
     CDS             2896..3636
                     /locus_tag="ACIUS0_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGB0219609.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3671..4192
                     /gene="ruvC"
                     /locus_tag="ACIUS0_001841"
     CDS             3671..4192
                     /gene="ruvC"
                     /locus_tag="ACIUS0_001841"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGB0219610.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4194..4796)
                     /locus_tag="ACIUS0_001842"
     CDS             complement(4194..4796)
                     /locus_tag="ACIUS0_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGB0219611.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4866..4931
                     /gene="yobI"
                     /locus_tag="ACIUS0_001843"
     CDS             4866..4931
                     /gene="yobI"
                     /locus_tag="ACIUS0_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HGB0219612.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5070..5681
                     /gene="ruvA"
                     /locus_tag="ACIUS0_001844"
     CDS             5070..5681
                     /gene="ruvA"
                     /locus_tag="ACIUS0_001844"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGB0219613.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5690..6700
                     /gene="ruvB"
                     /locus_tag="ACIUS0_001845"
     CDS             5690..6700
                     /gene="ruvB"
                     /locus_tag="ACIUS0_001845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGB0219614.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACIUS0_001846"
     CDS             complement(6847..7632)
                     /gene="znuB"
                     /locus_tag="ACIUS0_001846"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGB0219615.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACIUS0_001847"
     CDS             complement(7629..8384)
                     /gene="znuC"
                     /locus_tag="ACIUS0_001847"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGB0219616.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8463..9395
                     /gene="znuA"
                     /locus_tag="ACIUS0_001848"
     CDS             8463..9395
                     /gene="znuA"
                     /locus_tag="ACIUS0_001848"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGB0219617.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9411..10733
                     /gene="mepM"
                     /locus_tag="ACIUS0_001849"
     CDS             9411..10733
                     /gene="mepM"
                     /locus_tag="ACIUS0_001849"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGB0219618.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10853..11824
                     /gene="lpxM"
                     /locus_tag="ACIUS0_001850"
                     /gene_synonym="msbB"
     CDS             10853..11824
                     /gene="lpxM"
                     /locus_tag="ACIUS0_001850"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGB0219619.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACIUS0_001851"
     CDS             complement(11955..13397)
                     /gene="pyk"
                     /locus_tag="ACIUS0_001851"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGB0219620.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACIUS0_001852"
                     /gene_synonym="yebK"
     CDS             complement(13525..14394)
                     /gene="hexR"
                     /locus_tag="ACIUS0_001852"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGB0219621.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14731..16206
                     /gene="zwf"
                     /locus_tag="ACIUS0_001853"
     CDS             14731..16206
                     /gene="zwf"
                     /locus_tag="ACIUS0_001853"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGB0219622.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16441..18252
                     /gene="edd"
                     /locus_tag="ACIUS0_001854"
     CDS             16441..18252
                     /gene="edd"
                     /locus_tag="ACIUS0_001854"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGB0219623.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18289..18930
                     /gene="kdgA"
                     /locus_tag="ACIUS0_001855"
                     /gene_synonym="eda"
     CDS             18289..18930
                     /gene="kdgA"
                     /locus_tag="ACIUS0_001855"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGB0219624.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACIUS0_001856"
     CDS             complement(18986..20164)
                     /gene="purT"
                     /locus_tag="ACIUS0_001856"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGB0219625.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20298..20588
                     /gene="yebG"
                     /locus_tag="ACIUS0_001857"
                     /pseudo
     CDS             20298..20588
                     /gene="yebG"
                     /locus_tag="ACIUS0_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20655..21011
                     /gene="yebF"
                     /locus_tag="ACIUS0_001858"
     CDS             20655..21011
                     /gene="yebF"
                     /locus_tag="ACIUS0_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGB0219626.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21338..21997
                     /locus_tag="ACIUS0_001859"
     CDS             21338..21997
                     /locus_tag="ACIUS0_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGB0219627.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22207..24267
                     /gene="ptrB"
                     /locus_tag="ACIUS0_001860"
     CDS             22207..24267
                     /gene="ptrB"
                     /locus_tag="ACIUS0_001860"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGB0219628.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACIUS0_001861"
     CDS             complement(24264..24926)
                     /gene="exoX"
                     /locus_tag="ACIUS0_001861"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGB0219629.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACIUS0_001862"
                     /pseudo
     CDS             complement(24950..25606)
                     /gene="yobB"
                     /locus_tag="ACIUS0_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACIUS0_001863"
     CDS             complement(25708..25938)
                     /gene="holE"
                     /locus_tag="ACIUS0_001863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGB0219630.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26077..26451
                     /gene="yobA"
                     /locus_tag="ACIUS0_001864"
     CDS             26077..26451
                     /gene="yobA"
                     /locus_tag="ACIUS0_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGB0219631.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26455..27327
                     /gene="copD"
                     /locus_tag="ACIUS0_001865"
                     /pseudo
     CDS             26455..27327
                     /gene="copD"
                     /locus_tag="ACIUS0_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27340..27681
                     /locus_tag="ACIUS0_001866"
     CDS             27340..27681
                     /locus_tag="ACIUS0_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGB0219632.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYAINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28074..29151)
                     /locus_tag="ACIUS0_001867"
                     /pseudo
     CDS             complement(28074..29151)
                     /locus_tag="ACIUS0_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(29117..29398)
                     /locus_tag="ACIUS0_001868"
     CDS             complement(29117..29398)
                     /locus_tag="ACIUS0_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGB0219633.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7462 a         7262 c         7596 g         7258 t
ORIGIN      
        1 gcatcaaccg gctgttttaa tgcttcggcg taatcggcag accagccaac tcgcaccaga
       61 aacacgggct gaccgctggc gcgaaatttc gccgccagct tcccggcgcg attaactacc
      121 tcatcggcag tatgtggacc accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc
      181 accaacgcag tggttttagc attaagttca agcatgctga ctcctgaaaa aacaaaatcg
      241 tgcctcacac cttaatgcga ttagccgatg gcggaacgac aaattttgtt aatttttatg
      301 agttcgggca acacctttat agcaaactgg cgtagagtct gatttcaggc ccgatataaa
      361 gtggtaacga ggcgttccag cgcgggccag gctcttacgg tgcggcagaa tttccagtat
      421 aatagccgcc ttttttcatt cagttgtgac atacagctaa cgctgcgact tggtcacctg
      481 cgcagcaggg ggcatccgcc ggcagctaag ttaagggata tctcatgcgt acagaatatt
      541 gtggacagct ccgtttgtcc cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc
      601 gtcgtcgtga tcttggtagc ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc
      661 aggtattttt cgatccggat cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg
      721 agttctgcat tcaggttacg ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg
      781 atatggcgac cggcgaaatt gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag
      841 atgttctgcc gcttgactct aaccacgtca acaccgaaga agcgcgtctg aaataccgct
      901 acctcgacct gcgtcgcccg gaaatggcgc aacgcctgaa aacccgcgct aaaatcacca
      961 gcctggtgcg ccgttttatg gatgaccacg gcttcctcga catcgaaact ccgatgctga
     1021 ccaaagccac gccggaaggc gcgcgtgact acctggtgcc ttctcgtgtg cacaaaggta
     1081 aattctacgc actgccgcaa tccccacagt tgttcaaaca gctgctgatg atgtccggtt
     1141 ttgaccgtta ctatcagatc gttaaatgct tccgtgacga agacctgcgc gctgatcgtc
     1201 agcctgaatt tactcagatc gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg
     1261 aagtgatgga agcgctggtg cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg
     1321 atttcccggt aatgaccttt gcggaagcag aacgccgtta tggttctgat aaaccggatc
     1381 tgcgtaaccc gatggaactg accgacgttg ccgatctgct gaaatctgtt gagtttgcag
     1441 tatttgcggg gccagcgaac gatccgaaag gccgtgttgc cgctctacgt gttccgggtg
     1501 gcgcatcgct gacccgtaag cagatcgacg aatacggtaa cttcgttaaa atctacggcg
     1561 cgaaaggtct ggcttacatc aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca
     1621 gcccggtagc gaagttcctt aatgcagaaa tcatcgaagc catcctggat cgtactgccg
     1681 cgcaagatgg cgatatgatt ttcttcggtg ccgacaacaa gaaaattgtt gccgacggga
     1741 tgggcgcact gctcctgaaa gtgggtaaag accttggtct gaccgacgaa agcaaatggg
     1801 caccgctgtg ggttatcgac ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg
     1861 caatgcacca tccgttcacc tcaccgaaag atatgactgc tgcagaactg aaagctgcac
     1921 cggaaaatgc ggtggcgaac gcttacgata tggtcatcaa tggttacgaa gtgggcggtg
     1981 gttcagtacg tatccataat ggtgatatgc agcagacggt gtttggtatt ctgggtatca
     2041 acgaagagga acagcgcgag aaattcggct tcctgctcga cgccctgaaa tacggtactc
     2101 cgccgcacgc aggtctggca ttcggtcttg accgtctgac catgctgctg actggcaccg
     2161 acaatatccg tgacgttatc gccttcccga aaaccacggc ggcagcgtgt ctgatgactg
     2221 aagcaccgag ctttgctaac ccgactgcac tggctgagct gagcattcag gttgtgaaga
     2281 aggctgagaa taactgatat gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg
     2341 caactgagtg aactcccatg agcatagata actatgtgaa tgggatgagc gaaggcagtc
     2401 aacgaagagg cagcgtgaag gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca
     2461 tctatgcaca agatacgaaa cgggtgctga tgttgcagcg gcgtgacgat cccgatttct
     2521 ggcagtcggt aaccggcagc gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg
     2581 aagtaaagga agaggttacc attgatgttg tcgctgaaca actgacctta attgactgtc
     2641 agcgcacggt agagtttgaa attttttcac atttacgtca tcgctatgcg ccgggcgtga
     2701 cgcgtaatac ggaatcatgg ttctgtcttg cgcttccgca cgagcggcag atcgttttca
     2761 ctgaacatct ggcttacaag tggcttgatg cgcctgctgc ggcggcgctc actaagtcct
     2821 ggagcaaccg gcaggcgatt gaacagtttg taattaacgc tgcctgaaca ggcagggcta
     2881 tctggagata tttttatggc aggtcatagt aaatgggcca acaccagaca tcgtaaagct
     2941 gcgcaggatg ctaagcgcgg taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg
     3001 gctaagctgg gcggtggcga tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa
     3061 gcactgtcta acaacatgac ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc
     3121 ggtgatgatg atgcaaacat ggaaaccatc atctacgaag gttacggtcc tggcggcacg
     3181 gcaatcatga ttgaatgtct gtctgacaac cgcaaccgta ccgttgctga agtgcgtcac
     3241 gcattcagca aatgtggcgg taacctcggc actgacggtt ccgtagctta tctgttcagc
     3301 aaaaaaggcg tgatctcctt cgagaaaggc gacgaagaca ccatcatgga agcagctctg
     3361 gaagcaggtg ctgaagacgt tgtgacatat gatgacggcg cgattgatgt ctacactgca
     3421 tgggaagaaa tgggtaaagt gcgcgacgct ctggaagcgg caggtctgaa agcagacagc
     3481 gcggaagttt ccatgatccc gtccaccaaa gctgatatgg atgcagaaac cgcgccgaaa
     3541 ctgatgcgtc tgatcgatat gctggaagat tgcgacgacg tgcaggaagt ttaccataac
     3601 ggtgaaatct ctgatgaggt tgcagcgact ctctgatgat gcctgctaaa cagcaaaacg
     3661 gagacgcgtg atggctatta ttctcggcat tgacccgggt tcgcgcgtga ccggctacgg
     3721 cgtcatccgc caggtaggta ggcaactgtc ctacctgggt agcggatgca tacgcaccaa
     3781 agtggatgat ttaccgtctc gtctgaagct catctatgcg ggcgtgacgg aaatcatcac
     3841 ccagttccag cctgattatt tcgccattga acaagtcttt atggcgaaga acgctgactc
     3901 agccctgaaa ctgggccagg cgcgcggcgt ggcgattgtt gcggcggtga atcaggaatt
     3961 gccggtattt gaatacgcgg cacgtcaggt aaagcaaacg gtggtaggta tgggtagtgc
     4021 cgaaaaaagc caggtgcagc atatggtccg caccttgctg aaactgcccg ctaatccaca
     4081 ggcggatgcc gccgatgcgc tggcgattgc catcacccac tgccacgtta gtcagaatgc
     4141 gatgcagatg agcgaatcgc ggctgaacct gacgagaggg cgactgcgtt aagttatacc
     4201 gcctcggtca gttccggctg aggcgtttca actccctccg cgtgaatcaa caccaaaccc
     4261 ggatgatgcc acaaactggc tggcgtgacg gttttacgct cccacggaat agaacctaag
     4321 aaccagaatt tccagaaagt gagttttgca ttcggattgc tatttaacag ttgtccaaac
     4381 gtctctattt cacccaccgg aatacatagc gcctctttat aaatatcaaa gacaaatttt
     4441 gaacagaact ggcgcgaaga ttcgtattta aaacccgtgt ggtaaagttt gcgtagccgg
     4501 gagggaacct gttcaacaat tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc
     4561 tttatagcat agcgttgatt agacgaacgt ttaataaaac gagatagcgt ggtgatggtt
     4621 gaaaggggaa cccggctttc tgcaaccagg aagtcttcac cgttatgacc gataatgatc
     4681 ccgacgtgat tactccagca atttgatgca gctgatattt gaccaaataa ggcagcacct
     4741 atacatgtaa agacgatatc tccaatttca tattcagcag ggtaattaat attcacgttg
     4801 tcattccatt gaaatagata caaatatacg taatagcata aaaaaattat ttttcaggag
     4861 tcgcaatgta tatttttatt acgcatttct tcactgaata tgtaatattc aaatatttgc
     4921 ttccaatata acctgttgaa taaattatac tgtgccattt ttcagatcat cgggacacct
     4981 cgcaagtttt cttcatcctt cgctggatat ctatccagca tttttttatc atacagcatt
     5041 atctttgatt cattacgcag gagcgtcatg tgataggcag actcagaggc atcatcattg
     5101 aaaaacaacc cccgctggtg ttaattgaag tgggcggcgt aggctatgaa gtgcatatgc
     5161 cgatgacctg tttttatgaa ctccctgaag cgggtcagga agcgatcgtt ttcacccact
     5221 ttgtggtgcg tgaagacgcg caactgctgt acggttttaa caataaacaa gaacgcacgt
     5281 tgttcaaaga gttgatcaaa accaacggcg tcggcccgaa gttggccctg gcgatcctct
     5341 ccggaatgtc agcgcagcag ttcgttaatg ccgttgagcg tgaagaagtg ggggcactgg
     5401 tgaaactgcc gggtattggc aaaaaaaccg ccgaacgctt gattgttgaa atgaaagacc
     5461 gatttaaagg tttgcatggc gatctcttta cgccagccgc cgacctggta ctcacgtctc
     5521 ctgctagccc ggcgaccgac gatgctgaac aagaagcagt tgccgcgctg gtggcgctag
     5581 gctataaacc acaagaagca agccgcatgg tgagcaaaat cgctcgccct gacaccagca
     5641 gtgaaacatt aattcgcgaa gccctacgcg ccgcgttatg aggtaaagga tgattgaagc
     5701 agaccgtctg atttctgccg gtaccacttt gccggaagat gtagcagatc gcgccattcg
     5761 ccctaaatta ctggaagagt atgttggtca gccgcaggtt cgttcacaga tggagatttt
     5821 catcaaagca gcgaaactgc gcggcgatgc cctcgatcat ttgttgattt ttggtcctcc
     5881 ggggttgggt aaaactacgc ttgccaacat tgtcgccaat gaaatgggcg ttaatttacg
     5941 cacgacttct ggtccggtgc tggaaaaggc gggcgatttg gctgcgatgc tcactaacct
     6001 tgaaccgcat gacgtgctgt ttattgatga gatccaccgt ttatcgccag ttgttgaaga
     6061 agtgctgtac ccggcaatgg aagactacca actggatatc atgattggtg aaggtccggc
     6121 ggcacgctcc attaaaattg atttgccgcc gtttaccctg attggcgcaa ccacgcgcgc
     6181 aggttcgctg acatcaccgt tgcgcgaccg ttttggtatt gtgcaacgtc tggagtttta
     6241 tcaggtgccg gatctgcaat atatcgtcag tcgcagtgca cgctttatgg ggcttgagat
     6301 gagtgatgac ggtgcgcagg aagtagctcg tcgcgctcgc ggtacgccac gtattgccaa
     6361 ccgtctgctg cgtcgagtgc gtgatttcgc cgaagtgaag cacgatggca ccatctcggc
     6421 agatatcgct gctcaggcgc tggatatgtt gaatgtcgat gctgaaggtt tcgattatat
     6481 ggaccgcaaa ttgttgctgg cggtaatcga taagttcttt ggtgggccgg taggtctgga
     6541 taacctggcg gcagccattg gcgaagaacg tgaaaccatt gaagatgtac tggaacctta
     6601 tttgattcag caaggctttt tgcagcgtac accgcgcggg cgtatggcga cgacgcgggc
     6661 gtggaatcac tttggcataa cgccgccaga aatgccgtaa gtcggattgg tcgctcgcgc
     6721 cgcatccgac actggcagtg tgtctgatgc gacactgttc gcgtcttatc aggccagcca
     6781 gagatagcga accgtaggtc ggataaggcg ctcgcgccgc atccgacaaa tgtgttcagc
     6841 gatagattag ctggcctgct ttttcatcat actgataata aataacagtg ccgcacatag
     6901 caccaccgac ggacccgccg gcgtatcgta aaacgcggaa aaggttaaac cgccagttac
     6961 tgccaccatc cccaccaaaa cagcgacacc agccatctgt tccggcgtac gggcaaagcg
     7021 acgcgcagta gctgcaggaa taatcagcaa cgaagtaata atcaacgcac cgacaaattt
     7081 catcgctaca ccaatcgtca atgccgtcac cagcatcaac aacaatttca cttgctgtaa
     7141 tttcacacca tcaacaaacg ccagatccgg gctaatcgtc atcgacagca aattgcgcca
     7201 ttgccagaac aaaatagcca ccacgatgac cacgccaatc gcaatagaga tgagatcttc
     7261 tggcgtcact gccagtaaat caccgaacag gtaagccatc aaatcaacac gaatgttaga
     7321 catcagacta acgaccacta ggcccagcga cagggcactg tgcgccataa tccctaataa
     7381 cgtgtcgatc gccagctgtg gacgcttctc cagccatacc agaccgcccg ccagcagcag
     7441 cgtaacggca atcaccgcat agaatggatt cacgtccagc aacaaaccaa acgcgacacc
     7501 aagtaatgag gcatgagcca gcgtatcacc gaaataagac atacgacgcc agactacaaa
     7561 cgaacccagc ggacccgcgg cacaggcgag catgatcccg gctaaccaac cgggaaataa
     7621 taattcaatc atgagcgatc atttccccga cgcaaaacaa ttcgtccctg taaatcgtga
     7681 cgatgattat gatgatggcg atagataccc agttgttcag caccacgagg accaaacatt
     7741 gaaataaact ccggatgcag ggaaacaact tccggtgtgc cggaacaaca aatgtggtga
     7801 ttcaggcaca gcacttcatc ggtttttgcc atcaccagat gcagatcgtg agaaaccatt
     7861 aaaacgccac aatccagttc acgacgcaac tggtcaataa ggtcatataa cgccacttgg
     7921 ccatttacat ccacgccttg agtgggttca tccagcacta ataattgcgg gcaatttaac
     7981 aatgctcgcg ctaatagtac acgctgcgtt tcgccaccag agagcttttg catcggtgcg
     8041 ttaatcaggt gcccggcctg gacacgtttc agtgcaggca aaatatcttc tttatgtgta
     8101 ccagggcgta agcgtaaaaa acggtttacg gtcagtggca acgtggtgtc gagatacagc
     8161 ttctgcggta catagccgat gcgcagtttt ccgttgcgct tgataacccc ttcatcgggt
     8221 gttaccagcc cgagcactac ccgtaccagt gtcgacttac ctgcgccatt tggcccaagt
     8281 aaagtcaaaa tttttccagg tttaagttcc agcgacacat cagagaggac gcggcgttgg
     8341 ccaaaagaaa ccgagacatt ttccagggaa accagacttg tcatgttaat tttagtcttg
     8401 cagtagtcat gaaatgttat aatatcacac ttctcatatt cattacgatg attggtcgca
     8461 ttatgttaca taaaaaaacg cttcttttcg cagcattatc cgccgctctc tggggaggtg
     8521 caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa acccgttggg ttcatcgctt
     8581 ctgccattgc tgatggtgta acagaaacgg aggttttact gcctgacggc gcttcagaac
     8641 atgattattc actgcgtcca tcggatgtaa aacgcttaca gaacgcggac ttagtcgttt
     8701 gggttggccc ggagatggaa gcgtttatgc aaaaaccggt aagcaaatta ccagaagcga
     8761 agcaggtaac gattgcgcag cttgaagatg tgaaaccgct actgatgaaa agtattcacg
     8821 gcgatgatga tgatcacgac cacgcggaaa aaagtgacga agatcaccat cacggcgatt
     8881 tcaacatgca tctttggctt tccccagaga tagcgcgggc tacagcggtt gcaatccatg
     8941 gaaaattagt ggaacttatg ccgcaaagtc gagccaaact tgacgccaac ctgaaggatt
     9001 ttgaggcaca attggcctca accgaaacgc aggttggtaa cgagctcgcg ccgctcaagg
     9061 ggaaaggtta tttcgttttt cacgatgctt acggctattt cgaaaaacag ttcggactga
     9121 caccgcttgg tcattttacc gttaaccctg agattcaacc tggcgcgcag cgtttacatg
     9181 aaataagaac acagttggtt gagcaaaaag caacctgcgt ttttgctgag ccacagttca
     9241 ggccagcggt cgttgagagc gtcgcacgag ggacatccgt tcgtatggga acgttggatc
     9301 ccctggggac gaatatcaaa ctgggtaaaa caagctattc agaattcctg agtcaattag
     9361 ccaaccagta tgcgagctgc ctgaaaggag attaatgagg aagtgattac gtgcaacaga
     9421 tagcccgctc tgtcgccctg gcgtttaata atttaccgcg accacaccgc gttatgttgg
     9481 ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg gccctatgtt tatcaccgtg
     9541 atgccacgcc aattgtcaaa accattgagc tggaacagaa cgaaattcgt tcgctcttac
     9601 ctgaagccag tgagccgatt gatcaagctg cacaagaaga tgaagccatt ccccaggacg
     9661 aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga atatgttgtt tccactggcg
     9721 atacgctaag cagcattctc aatcagtatg gtattgatat gggtgatatc acccaacttg
     9781 ctgctgccga caaagaattg cgtaacctga aaatcggtca acaactctcc tggacattaa
     9841 ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc tcgtcgtgaa acccgaacct
     9901 atgaccgtac tgccgctaac ggttttaaaa tgaccagcga aatgcagcaa ggagagtggg
     9961 ttaacaatct gctgaaaggt accgtcggag ggagctttgt tgccagcgcc agaaacgccg
    10021 gtttaaccag cgccgaagtg agcgcagtga ttaaagccat gcagtggcaa atggatttcc
    10081 gcaaactgaa aaaaggcgat gaatttgcgg tattaatgtc tcgagaaatg cttgatggta
    10141 aacgtgagca aagccagctg ctgggcgtac gtttgcgttc agaaggtaaa gattattacg
    10201 caatccgcgc tgaggatggc aaattctacg atcgtaacgg tactggtctg gcgaaaggat
    10261 tcttgcgatt cccgacggcg aaacagttcc gtatctcgtc taactttaac ccgcgtcgaa
    10321 ctaatccggt gacaggtcgc gttgcaccac acagaggtgt tgatttcgcc atgccacaag
    10381 gtacgccagt gctttcagtg ggtgacggtg aagtggtggt tgccaaacgc agtggcgcag
    10441 caggttatta tgtggctatt cgtcatggtc gcagctacac cacgcgttat atgcacttgc
    10501 gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg cgaccgtatc gcgctttccg
    10561 gtaataccgg acgttcaact gggccgcatc tgcactatga agtatggata aaccagcagg
    10621 ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg gctgaccggc tccgatcgtc
    10681 gcgaattcct ggcgcaggcc aaagagattg tgccgcagct acggtttgat taattaacat
    10741 ccattcgcag ccggtacgca gtcagtaccg gcttttttta tttggtgcgg ggcaagttgt
    10801 gccgctacac tatcaccaga ttgatttttg ccttatccga aactggaaaa gcatggaaac
    10861 gaaaaaaaat aatagcgaat acattcctga gtttgataaa tcctttcgcc acccgcgcta
    10921 ctggggagca tggctgggtg tagcagcgat ggcgggtatc gctttaacgc cgccaaagtt
    10981 ccgtgatccc attctggcac ggctgggacg ttttgccgga cgactgggaa aaagctcacg
    11041 ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa cgtagtgaag ctgaacgcga
    11101 agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg atggcaatga tggctgagtt
    11161 ggcaatacgc gggccggaga aaattcagcc gcgcgttggc tggcaagggc tggagatcat
    11221 cgaagagatg cggcgtaata acgagaaagt tatctttctg gtgccgcacg gttgggccgt
    11281 cgatattcct gccatgctga tggcctcgca agggcagaaa atggcagcga tgttccataa
    11341 tcagggcaac ccggtttttg attatgtctg gaacacggtg cgtcgtcgct ttggcggtcg
    11401 tctgcatgcg agaaatgacg gtattaaacc attcatccag tcggtacgtc aggggtactg
    11461 gggatattat ttacccgatc aggatcatgg cccagagcac agcgaatttg tggatttctt
    11521 tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg atgaaagtat gccgtgcgcg
    11581 cgttgtaccg ctgtttccga tttatgatgg caagacgcat cgtctgacga ttcaggtgcg
    11641 cccaccgatg gatgatctgt tagaggcgga tgatcatacg attgagcggc ggatgaatga
    11701 agaagtcgag atttttgttg gtccgcgacc agaacaatac acctggatac taaaattgct
    11761 gaaaactcgc aaaccgggcg aaatccagcc gtataagcgc aaagatcttt atcccatcaa
    11821 ataaaaaagc ctctcgcgag gagaggcctt cgcctgatga taagttcaag tttgcttcag
    11881 aatattcgaa atctgttgaa ctatcattga actataggcc ggatgtggcg ttttcgccgc
    11941 atccggcaac gtacttactc taccgttaaa atacgcgtgg tattagtaga acccacggta
    12001 ctcatcacgt cgccttgggt gacaatcacc aggtcaccag acatcaagta acctttatcg
    12061 cgcagcagat taaccgcttc gctggcagct gctacgccgt cattagcgct atcaaagggc
    12121 accggcgtaa cgccacgata gagagcagtc aggttcagcg tacgttcatg gcgcgacatg
    12181 gcgaaaattg gtagaccaga gctgatacgg gaggtcatca gcgcggtacg acccgattcg
    12241 gtcatggtaa tgatcgccgt aacgcccttc aggtggttag ctgcgtacat tgccgacatg
    12301 gcaatagctt cttccacatt gtcgaactga acgtccagac ggtgtttaga aacgttgatg
    12361 ctcgggattt tttccgcccc caggcaaacg cgcgccatgg ctgcaacagt ttctgacgga
    12421 tactgcccag cggcagtttc tgcagacagc atcacagcgt cagtaccatc cagaacggcg
    12481 tttgctacgt ccatgacttc tgcacgcgtc ggcatcgggt tagtaatcat tgactccatc
    12541 atctgggtcg ccgtgattac cgctcggttt agctgacgcg cacgacggat caacgctttc
    12601 tgaatgccga ccagttccgg gtcgccaatt tccacaccga ggtcgccacg tgcaaccatt
    12661 accacgtcag aggcgaggat gatgtcatcc attgcatcct ggctgcaaac ggcttccgca
    12721 cgttcaacct tggcaacaat tttcgcatca catcctgcat cgcgtgccag gcgacgggca
    12781 tagttcagat cttcgccaca gcgtgggaag gagacagcca ggtaatctac gccaatcaac
    12841 gccgcagtct taatgtctgc tttgtctttt tcggtcagcg cttcagccga caaaccgccg
    12901 ccaagtttgt tgataccttt attgttggag aggggaccac cgacggtgac ttcggtgaac
    12961 actttcatgc cctgaacttc cagtactttt aactggacgc gaccatcgtc cagcagcagg
    13021 atgtcaccag gcacgacgtc agtaggcagg cctttgtagt cgataccgac tttttctttg
    13081 tcgccttcac ctttacccag gttggcgtcg agcaggaatt tatccccaat attgaggaaa
    13141 actttgcctt ctttaaaggt ggatacacgg attttgggcc cctggaggtc acccagaata
    13201 gccacatgac gccccagttt tgcggcaatc tcacgaactt tatccgcgcg cattttgtga
    13261 tcttcaggcg agccgtgaga aaagttcata cgtacaacgt tggcacccgc cgcgataact
    13321 ttttcaagat tattatcgcg atctgttgct gggcctaacg tggtaacgat ttttgttctg
    13381 cgaagccttt tggacatgta atactccgtt gactgaaaca accaggtgtt gcttgaacat
    13441 gaaatccgaa tgaaataacg ccgcgatggc gataaattaa tctcattcgg atgtcgtata
    13501 agattaggac agtgacagtc gcttttagcg atcgtcactt aaattaagta actgcttatc
    13561 aaaacgcgat tccttcagcg cttccttgac ccgcttcaag ttatctctga attttgcacc
    13621 gcgtcgcaaa gtaaatcctg tcgccagcac atctatcacg gtcagctgtg caagtcgaga
    13681 aaccatgggc atataaatgt cagtatcttc cggtacgtcg agggtaattg ccagcgttgc
    13741 ttcccgggcg agcggggtac ccgcagaggt gagggcaatc accatggcgt cgttttcgcg
    13801 tgccagctgc gccagctcga ccagattttt tgttcttcct gtgtgagaaa tcagcaccac
    13861 cacgtctccg tcgctacaat tcatacaact catgcgttgc agcacgatat catcggagta
    13921 caccaccgga acattaaaac gaaagaactt attcatcgca tcgtgggcaa cggcggcaga
    13981 agagcctaat ccgaaaaagg cgattttttt tgcctgagtg agcaagtcga cggcgcggtt
    14041 gatggcagat ttatccagtg aatgacggac atgatcgagc gttgccattg cggactcaaa
    14101 tattttcccc gtgtatgatt caacgctgtc atcttcattg acgttgcgat taacataggg
    14161 agtgccattc gccagacttt gtgccagatg aagtttaaaa tcaggaaaac cgcgcgtgtc
    14221 catgctgcga cagaaacgat tcaccgtcgg ttcgctaaca ttggcttcca gcgccagagc
    14281 agcaatactc gaatggatcg cgttatcggg cgaagccaga atgacctcgg caactttgcg
    14341 ctctgatttg ctcaaatgtt ccagctgaga ctggattttt tccagcatat tcatgatgta
    14401 aagagactca cgggtaatga cgatttccgc actgaaagaa atcgaaatgc agttttgtca
    14461 gatattacgc ctgtgtgccg tgttaatgac aaaagcagat aaaaaagttg ttattttttt
    14521 cataacatga tcagtgtcag atttttaccg aatggaaaac gatgattttt ttatcagttt
    14581 tgccgcgctt tgcgcgcttt tcccgtaatc gcacgggtgg ataagcgttt acagttttcg
    14641 caagctcgta aaagcagtac agtgcaccgt aagaaaatta caagtatacc ctggcttaag
    14701 taccgggtta gttaacttaa ggagaatgac atggcggtaa cgcaaacagc ccaggcctgt
    14761 gacctggtca ttttcggcgc gaaaggcgac cttgcgcgtc gtaaattgct gccttccctg
    14821 tatcaactgg aaaaagtcgg tcagctcaac ccggacaccc ggattatcgg cgtagggcgt
    14881 gctgactggg ataaagcggc atataccaaa gttgtccgcg aggcgctcga aactttcatg
    14941 aaagaaacca ttgatgaagg tttatgggac accctgagtg cacgtctgga tttttgtaat
    15001 ctcgatgtca atgacactgc tgcattcaac cgtctcggcg cgatgctgga tcaaaaaaat
    15061 cgtatcacta ttaactactt tgccatgccg cccagcactt ttggcgcaat ttgcaaaggg
    15121 cttggcgagg caaaactgaa tgctaaaccg gcacgcgtag tcatggagaa accgctgggg
    15181 acgtcgctgg cgacctcgca ggaaatcaat gatcaggttg gcgaatactt cgaggagtgc
    15241 caggtttacc gtatcgacca ctatcttggt aaagaaacgg tgctgaacct gttggcgctg
    15301 cgttttgcta actccctgtt tgtgaataac tgggacaatc gcaccattga tcatgttgag
    15361 attaccgtgg cagaagaagt ggggatcgaa gggcgctggg gctattttga taaagccggt
    15421 cagatgcgtg acatgattca gaaccacctg ctacaaattc tctgcatgat tgcgatgtct
    15481 ccgccatctg acctgagcgc agacagcatc cgcgatgaaa aagtgaaagt actgaagtcc
    15541 ctgcgccgca tcgaccgctc caacgtacgc gaaaaaaccg tacgcgggca atatactgcg
    15601 ggcttcgccc agggcaaaaa agtgccggga tatctggaag aagagggcgc gaacaaaagc
    15661 agcaatacag aaaccttcgt ggcgatccgc gtcgacattg ataactggcg ctgggccggt
    15721 gtgccattct acctgcgtac tggtaaacgt ctgccgacca aatgttctga agtcgtggtc
    15781 tatttcaaaa cacctgaact gaatctgttt aaagagtcgt ggcaggatct gccgcagaat
    15841 aaactgacta tccgtctgca acctgatgaa ggcgtggata tccaggtact gaataaagtt
    15901 cctggccttg accacaaaca taacctgcaa atcaccaagc tggatctgag ctattcagaa
    15961 acctttaatc agacgcatct ggcggatgcc tatgaacgtt tgctgctgga aaccatgcgt
    16021 ggtattcagg cactgtttgt acgtcgcgac gaagtggaag aagcctggaa atgggtagac
    16081 tccattactg aggcgtgggc gatggacaat gatgcgccga aaccgtatca ggccggaacc
    16141 tggggacccg ttgcctcggt ggcgatgatt acccgtgatg gtcgttcctg gaatgagttt
    16201 gagtaatatc agcgcttatc ctttatggtt attttaccgg taacatgatc ttgcacagat
    16261 tgtagaacaa tttttacact ttcaggcctc gtgcggattc acccacgagg ctttttttat
    16321 tacactgact gaaacgtttt tgccctatga gctccggtta caggcgtttc actcataaat
    16381 cctctgaatg aaacgcgttg tgaatcatcc tgctctgaca actcaatttc aggagccttt
    16441 atgaatccac aattgttacg cgtaacaaat cgaatcattg aacgttcgcg cgagactcgc
    16501 tctgcttatc tcgcccggat agaacaagcg aaaacgtcga ccgttcatcg ttcgcagttg
    16561 gcatgcggta acctggcaca cggtttcgct gcctgccagc cagaaaacaa agcctctttg
    16621 aaaagcatgt tgcgtaacaa tatcgccatc atcacctcct ataacgacat gctctccgcg
    16681 caccagcctt atgaacacta tccagaaatc attcgtaaag ccctgcatga agcgaatgcg
    16741 gttggtcagg ttgcgggcgg tgttccggcg atgtgtgatg gtgtcaccca ggggcaggat
    16801 ggaatggaat tgtcgctgct aagccgcgaa gtgatagcga tgtctgcggc ggtggggctg
    16861 tcccataaca tgtttgatgg tgctctgttc ctcggtgtgt gcgacaagat tgtcccgggt
    16921 ctgacgatgg cagccctgtc gtttggtcat ttgcctgcgg tgtttgtgcc gtctggaccg
    16981 atggcaagcg gtttgccaaa taaagaaaaa gtgcgtattc gccagcttta tgccgaaggt
    17041 aaagtggacc gcatggcctt actggagtca gaagccgcgt cttaccatgc gccgggaaca
    17101 tgtactttct acggtactgc caacaccaac cagatggtgg tggaatttat ggggatgcag
    17161 ttgccaggct cttcttttgt tcatccggat tctccgctgc gcgatgcttt gaccgctgcc
    17221 gctgcgcgtc aggttacacg catgaccggt aatggtaatg aatggatgcc gatcggtaag
    17281 atgatcgatg agaaagtggt ggtgaacggt atcgttgcac tgctggcgac cggtggttcc
    17341 actaaccaca ccatgcacct ggtggcgatg gcgcgcgcgg ccggtattca gattaactgg
    17401 gatgacttct ctgacctttc tgatgttgta ccgctgatgg cacgtctcta cccgaacggt
    17461 ccggccgata ttaaccactt ccaggcggca ggtggcgtac cggttctggt gcgtgaactg
    17521 ctcaaagcag gcctgctgca tgaagatgtc aatacggtgg caggctttgg tctgtctcgt
    17581 tatacccttg aaccatggct gaataatggt gaactggact ggcgggaagg ggcggaaaaa
    17641 tcactcgaca gcaatgtgat cgcttccttc gaacaacctt tctctcatca tggtgggaca
    17701 aaagtgttaa gcggtaacct gggccgtgcg gttatgaaaa cctctgccgt gccggttgag
    17761 aaccaggtga ttgaagcgcc agcggttgtt tttgaaagcc agcatgacgt tatgccggcc
    17821 tttgaagcgg gtttgctgga ccgcgattgt gtcgttgttg tccgtcatca ggggccaaaa
    17881 gcgaacggaa tgccagaatt acataaactc atgccgccac ttggtgtatt attggaccgg
    17941 tgtttcaaaa ttgcgttagt taccgatgga cgactctccg gcgcttcagg taaagtgccg
    18001 tcagctatcc acgtaacacc agaagcctac gatggcgggc tgctggcaaa agtgcgcgac
    18061 ggggacatca ttcgtgtgaa tggacagaca ggcgaactga cactgctggt agacgaagcg
    18121 gaactggctg ctcgcgaacc gcacattcct gacctgagcg cgtcacgcgt gggaacagga
    18181 cgtgaattat tcagcgcctt gcgtgaaaaa ctgtccggtg ccgaacaggg cgcaacctgt
    18241 atcacttttt aagacgacaa atttgtaatc aggcgagaga aaactctgat gaaaaactgg
    18301 aaaacaagtg cagaatcaat cctgaccacc ggcccggttg taccggttat cgtggtaaaa
    18361 aaactggaac acgcggtgcc gatggcaaaa gcgttggttg ctggtggggt gcgcgttctg
    18421 gaagtgactc tgcgtaccga gtgtgcagtt gacgctatcc gtgctatcgc caaagaagtg
    18481 cctgaagcga ttgtgggtgc cggtacggtg ctgaatccac agcagctggc agaagtcact
    18541 gaagcgggtg cacagttcgc aattagcccg ggtctgaccg agccgctgct gaaagctgct
    18601 accgaaggga ctattcctct gattccgggg atcagcactg tttccgaact gatgctgggt
    18661 atggactacg gtttgaaaga gttcaaattc ttcccggctg aagctaacgg cggcgtgaaa
    18721 gccctgcagg cgatcgcggg tccgttctcc caggtccgtt tctgcccgac gggtggtatt
    18781 tctccggcta actaccgtga ctacctggca ctgaaaagcg tgctgtgcat cggtggttcc
    18841 tggctggttc cggcagatgc gctggaagcg ggcgattacg accgcattac taagctggcg
    18901 cgtgaagctg tagaaggcgc taagctgtaa aagtcaaaat gcccgatcta ggatcgggca
    18961 tttttgtagc gtttttttgc cgggtttaac cctgtacttt tacctgtccg gcggcgtgct
    19021 tcgcgcgttc aatggcgtca acaacactct ctgaagtagc cagtaccacc cccagatgac
    19081 ggctgccatc aatttccggc ttaccaaata aacgaatctg caaatctgcg cctacggcat
    19141 tctgcacatt atcaaacgtg acattctgac tggtcagttg tggcagaata acggcagaag
    19201 ctgcaggacc atactgacgg atcccgccaa caggaagtcc gaggaaggca cgtacatgca
    19261 gggcaaactc tgagagatct tgagaaatta acgtcaccat cccggtatca tgtggacgag
    19321 gggagacctc actgaaaatc acctcatcac cacagacaaa cagctcgaca ccaaacagac
    19381 cataaccgcc cagtgccagc accactttac gggcaatctc ctgcgcacgt tcaagggcaa
    19441 gcgggctcat ttgctgtggt tgccaggatt cacggtagtc gccatcttcc tggcgatgac
    19501 ctactggtgc acagaaatgg acgccatcca ccgcgctgac ggttagcagg gtaatttcga
    19561 agtcaaactt aacgacgcct tcaacaatta cgcggcccgc tccggcgcga ccgccttgct
    19621 gagcgtattc ccatgcctga gcaagttgct ctgcagagcg aataaacgtc tgccccttgc
    19681 cggaagagct catcaccggt tttacaatgc agggatagcc aatggcggca accgcctcgc
    19741 ggaaaaggct tttgctatcg gcaaaacgat aagtggaagt gggcagctgc agctcttctg
    19801 ccgccaggcg acggataccc tcgcgattca tcgttaattt cgttgcgcga gcgcagggga
    19861 caacattcag tccttcctct tcaagttgga tcagcatatc ggtggcaata gcttcgatct
    19921 ccggcacgat ataatgtggt ttttccagtt caaccacacg gcgtaatgca tcaccatcaa
    19981 gcatattaat gacatgggag cgatgcgcga catgcatggc tggtgcgtcg gcatagcgat
    20041 cgacggcaat cacctctacg ccgagacgct gacactcgat tgccacttct ttacccagtt
    20101 caccggagcc taataacatc acgcgagttg ctgccggacg cagcgcagtg cctaataacg
    20161 tcataacgaa tatcccctga ttaattccgc gcgcagtata aacgaaaacg tttgcgtgtg
    20221 tctttattgc tgatgttgat ttctcaaccg aaaagaaata tactgtataa aatctcagtt
    20281 attatgagag gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt gagggcgaag
    20341 agaaaatgtc gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg cttgatacgg
    20401 cggatctgct tgacacctag ctgacaaatt ctccagtgca aatggaagac gagcaacgtg
    20461 aagccctttc gctatggctg gcagaacaaa aagaggtgct gagcaccatt ctgaaaaccg
    20521 gcaaattacc gtccccacag gtcgtcggtg ctgaatccga agaggaagat gcctcacacg
    20581 cggcctgaag caacttgcgc tcctgacaat ttgtaccatg ctttctcctg aatcattgtg
    20641 ctatggagaa aaacatgaaa aaaagagggg cgtttttagg gcggttgttg gtttctgcct
    20701 gcgcatcagt tttcgctgcc aataatgaaa ccagcaagtc ggtcactttc ccaaagtgtg
    20761 aaggtctgga tgctgccgga attgccgcga gcgtaaaacg tgattatcaa caaaatcgcg
    20821 tggcgcgctg ggcagatgat caaaaaattg tcggtcaggc cgatcccgtg gcctgggtca
    20881 gtttgcagga cattcagggt aaagatgata aatggtcagt accgctaacc gtgcgtggta
    20941 aaagtgccga tattcattac caggtctgcg tggactgcaa agcgggaatg gcggaatatc
    21001 agcggcgtta atggcttctg ataattcgat tgaccgttgg cggatgcggc gcgagtgcct
    21061 tgtccgtccc agaacgcacc acaatcgtag gcctgagaag acgcaacagc gtcgcatcgg
    21121 gcaatcagca tcaaacaatg ccactcaaaa cgacaaacta ctttcaccta aaataccctt
    21181 atcacctgat atacagtaaa taccttacct ggcgcgaatt tctttgaata acggcctacc
    21241 gccccgctta tttgcgcatc ttttgtctcg atatacattt tctgacatcc tcatccgcta
    21301 tgctacaggc actaaaaatg gttattgagg agcaaatatg gctaactggt taaatcaact
    21361 gcaatccctg cttgggcaaa gcagttcttc tacctcctcg tctgcggatc agggattggg
    21421 caaactgtta gtgtcaggcg cattaggcgg actggctggg ctgctggtcg caaataaatc
    21481 agcacgtaaa cttcttacga aatatggcac caacgcgtta ctggttggcg gcggagcggt
    21541 agcgggtacg gtgctgtgga ataaatacaa agataaaatt cgcgcggcgc atcaggacga
    21601 accgcagttt ggcgcgcaaa gtacgccgct ggatgagcgt acggaacgtt tgatccttgc
    21661 gctggtcttt gccgctaaaa gtgatggtca tattgatgcc aatgaacgtg cggcaatcga
    21721 ccagcaattg cgtgaagccg gcgtggaaga gcaggggcgt gtactcattg agcaggcaat
    21781 cgaacaaccg ctggatccac aacgcctggc taccggggtc cgcaatgaag aagaggcgct
    21841 ggaaatctat ttcctgagct gcgcggctat tgatattgac cattttatgg aacgcagtta
    21901 cctgaatgca ctgggtgacg cgctgaaaat cccccaggat gttcgtgaag gcattgaacg
    21961 cgatctcgaa cagcaaaaac gcactttggc ggaataattc cgctctctgg atagcctgaa
    22021 gttgactatt tcgggctatc cagaaccgca cagccatttg aacaggctga ttttctctgt
    22081 ctgcataaca ttcgatatcc gcgccattac ctgtcatcat ctaagcaatg acgaccctgt
    22141 ttcgcttgca tccccggtga gttttgccac ccttataaga tgtttccaac cagaaagaac
    22201 aataacatgc taccaaaagc cgcccgcatt ccccacgcca tgacgcttca tggcgatacg
    22261 cgcatcgata attactactg gctgcgggac gatacgcgtt ctcagccaaa agtcctggac
    22321 tacctgcaac aagaaaatag ttacggtcat cgggtgatgg cctcacaaca agccttgcag
    22381 gatcgcatct taaaggaaat catcgaccgc attccgcaaa gagaagtttc tgcgccctac
    22441 ataaaaaatg gctaccgcta tcggcatatt tatgaaccag gctgtgaata tgctatctac
    22501 cagcgtcaat cggcattcag cgaagagtgg gatgagtggg aaacattgct cgatgccaac
    22561 aagcgcgcag ctcatagtga gttttattcg atgggcggaa tggcgattac gcccgataac
    22621 accattatgg cgctggcaga agattttctt tcccgacgcc agtacggcat tcgttttcgt
    22681 aatctggaaa caggtaactg gtacccggaa ctgctggata acgttgaacc cagctttgtc
    22741 tgggcaaatg actcctggac tttctactat gttcgcaagc atccggtgac gctgctgcct
    22801 tatcaggtct ggcgtcacgc catcggtacg ccagcatcgc aagatacact gatctacgaa
    22861 gaaaaagacg atacctatta tgtcagcctg cataaaacga cgtcgaagca ctatgtcgtc
    22921 attcatctgg ccagcgccac caccagtgaa gttcgcctgc tggacgcgga aatggccgat
    22981 gccgagccgt ttgtttttct gccgcgccgc aaagatcacg aatacagcct tgatcactac
    23041 cagcatcgtt tttatctgcg ttccaatcgc cacggcaaaa actttggctt ataccgtacc
    23101 cgtatgcgtg atgagcaaca gtgggaagag ttaattccgc cacgcgaaaa catcatgctg
    23161 gaagggttta cgctgtttac cgactggctg gtggttgaag agcgtcagcg cgggttaacc
    23221 agtttgcgcc aaattaaccg caagacccgg gaagtcattg gcattgcctt tgatgatccg
    23281 gcctatgtga cctggattgc ctacaatcca gaacctgaaa ccgcgcgatt gcgttatggt
    23341 tattcttcca tgaccacacc agacactttg tttgaactgg atatggatac cggtgagcgt
    23401 cgtgtattaa aacaaacaga agtgcctggt tttgatgcgg cgaattaccg cagtgaacac
    23461 ctgtggatag tcgcccgtga tggcgtcgaa gttccggttt cgctggtcta tcatcgcaaa
    23521 cattttcgca aaggacacaa cccgttgctg gtgtatggct atggttctta cggcgcaagt
    23581 attgatgccg atttcagttt tagccgcttg agtttgttag atcgtggctt tgtctacgcc
    23641 attgtccatg ttcgcggcgg tggtgagctg gggcaacaat ggtacgaaga cggtaaattt
    23701 ctgaagaaga aaaatacgtt taatgattat cttgatgcct gcgatgcatt gttaaaactg
    23761 ggctatggct ctccttcgct ctgttatgcg atgggcggaa gtgcgggggg catgttgatg
    23821 ggcgttgcaa ttaatcaacg cccggaatta ttccacggcg ttatcgccca ggtaccgttt
    23881 gttgatgttg taacaacgat gcttgatgaa tcaattcctc ttaccactgg tgagtttgaa
    23941 gagtggggaa atccgcagga tccgcaatat tacgagtaca tgaaaagcta cagcccgtat
    24001 gacaacgtca ccgcacaggc ttatccgcat ttactggtaa cgaccggttt acacgattct
    24061 caggtgcaat attgggaacc ggcaaaatgg gtcgctaaat tgcgcgagct gaaaaccgat
    24121 gaccatcttt tattgctctg taccgacatg gactcaggcc atggcggtaa atctggccgc
    24181 tttaaatcgt acgaaggcgt agcgatggaa tatgcttttc tggtcgcgct ggcgcaggga
    24241 acattacccg ctacgcctgc ggattaagta ttttccagat aatgtttcag tgttaaacgc
    24301 agctccgggc tcatgctgtc caggttatta aataaccagc gcagatagcc cggatagcgt
    24361 tcggcaacgt cggaaaccgc tttgccacgg tatttgccaa aggtgaaggt tgtcatcaac
    24421 gacggacgtc cggtgatatc ggccatctgt tctgccgtcc agccggaggt gttcataata
    24481 tcgataagca acgcggcggt gatataacag tcatacaacg cgcggtgatg atgcaggccc
    24541 agcggtgtct gtacattgag cttgcgtgtt ttatataacg ccatattgct gtacttgagc
    24601 cctggccaca aacgacgggc cagtttcata gtgcaaatcc actcaccggg catctcaggc
    24661 agtacgcggc ggtcaaagct ggcgttgtgc gcgacatacc attcactacc gtaatagtgt
    24721 gggatcacat cttcaatcca cggtttatcg gcgaccatgg cttcggtgat gcgatgaatc
    24781 gccatcgctt gtggactaat aggacgatcg gggcgcacca ggaggctcat ggggttgacg
    24841 atttttccgt caatgacatc aacagaggca atctcaacga tccctccctg caaaccgcag
    24901 gtttctgtat cgataatgcg caacatggca tgatccaggc cgaaaaagcc tagcgtaatg
    24961 gaatgatatc gccttgccaa ccctgtgatg aacgctgacc gactaacaat aatgaaccac
    25021 gatcggcgcg aacgatgaga cgaccatatt catcccatag tgcactgtta ccacgggcat
    25081 ttgccatcag cacggcaatc gagtactgat gtgaaaaaaa ttgtaagcga cgggccgacg
    25141 ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa cagtgaacag gtcgggtcca
    25201 tatccatacc ttgcggttgt tcatcgacca ccgtgatggt tctggaacga cggcccagac
    25261 atgcaccatg actctgatgg tagttcccag gcgtttttcg ccacggtgcg aacaccgcaa
    25321 ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc ggcaatgatg gtcattcgct
    25381 aggtcgttgc ggcataacaa agcgggtcta acagtgaaag atcgggtggg gcaggaaggg
    25441 cacgtcgcga ataatcacac cccagtaaag aaagcgatgg aaaaactaac agctgacatt
    25501 gttgcctggc ggcggccctc acaaactcca gatgatgagc tacctgttcg gtgagtgagg
    25561 ttttacgagg ctcatattgc gctgctgcaa ctttccagaa cgacataatg acttcctttt
    25621 caaaatgact gcaaattatg ggggcggttt agaaaatact cataatttag caggcgttat
    25681 gtaagaaagt gtaactctgt ataagtttta tttaagtttg ggctcgtaag gtaaacgtga
    25741 cagattgacc gaagccaaac ggtgggcaat aagccgctcg cgaaaccagc tgcgcaaatg
    25801 ttctggctgt tcacgttcaa ccgcttcagc gatcaccggc atattgtagc gttctttaaa
    25861 agccaccccg gccgccgcca aatcgacatt cactttatcc atttctgttt gatccagttt
    25921 agccagattc ttcagcattc ttaatctcct catcattcgc gggcaaaagt tactaagagt
    25981 gagcctggat ggcaagccgg aattgatgat cctcgactca gacattagac aaggttatgc
    26041 tctgtaaaaa agttattaca aaaaggaata gataatatgg cttcaactgc acgctccctt
    26101 cgctacgcgc tggcaatcct gaccacttcg ttggtgacac cttccgtctg ggcacatgcg
    26161 catttaacgc atcagtatcc tgcggcaaac gcgcaagtga cagctgcccc gcaggcaatc
    26221 actttaaact tctcggaagg cgttgaaaca ggattcagtg gtgcaaaaat cacggggcca
    26281 aaaaacgaaa atattaaaac attaccagcg aagcggaatg agcaggacca aaagcaactg
    26341 attgttccac tggccgattc gctgaaaccc ggaacctaca ccgttgactg gcatgttgtt
    26401 tcggtggacg ggcataaaac gaaaggacac tacaccttta gcgtgaaata aatcatgctg
    26461 gcgtttacct ggatcgcgct gcgatttatc catttcacct cgttgatgct ggtctttggc
    26521 tttgcaatgt atggtgcctg gctggccccc ttaatgattc gtcgcttgct gacgaagcgt
    26581 tctctgcgtt tacaacagca cgctgccgtc tggagtttga tcagtgccac tgcaatgctc
    26641 gccgttcagg gcgggttgat gggaacggga tggacagatg tattttcgcc aaacatttgg
    26701 caggctgttt tgcaaacgca gtttggtggc gtctggttat ggcaaattgt tctcgccctc
    26761 gtaacgttga tcgtcgccct tatgcaaccg cggaatatgc cacgtttgct gtttatgctc
    26821 actaccgcgc aatttattct gctggcggga gtggggtatg cgacgctgaa tgaaggggta
    26881 acagcgaaaa tccatcagac taatcacgcg attcacctga tttgtgcggc ggcgtggttt
    26941 gggggattac tgccggtgct ctggtgtatg caactcatca aaggccgttg gcgacactag
    27001 gctattcagg cgctgatgcg tttttcctgg tgcgggcatt ttgcggtgat cggcgtactg
    27061 gcaagcggcg tgcttaatgc attgttgatt accggatttc cccccacgct tacgacttac
    27121 tggggccaac tcttattgct caaagccatc ctggtgatga ttatggtggt cattgcgctg
    27181 gctaatcgtt atgttcttgt accgcgcatg aggcaggatg aagatcgtgc ggcaccctgg
    27241 tttgtgtgga tgactaaact cgaatgggcg ataggtgcgg tagtgctggt gatcatcagc
    27301 ctgcttgcga ctctcgaacc tttttgatgg attacgaaaa tgatgaaaaa aagtattctg
    27361 gcgtttctgt tactcaccag ttctgcagcg gcgctggcgg ctccacaggt gataaccgtg
    27421 agtcgttttg aagtgggtaa agacaaatgg gcgtttaatc gcgaagaggt aatgctgact
    27481 tgccgaccgg gtaatgcttt gtatgccatc aacccaagta ccctcgtgca gtatccttta
    27541 aacgatatcg cacaaaagga agttgccagt gggaagacca atgcccaacc catttcggtg
    27601 attcagattg atgatcctaa caatcccggc gaaaaaatga gtctggcacc gtttatagaa
    27661 cgagctgaaa aactctgtta attacctaaa atagcctttt gatttccaat aaaaaaaccg
    27721 cctcagttct ttcaccagaa cgggcggttt ttaacattta agctgatgag caccgcgctt
    27781 tttattgacc attttgcacg caaactggaa aacctggcgt cgtcatctat tcttaaaggg
    27841 caaggcaact aagcctgcat taatgccaac ttttagcgca cggctctctc ccaagagcca
    27901 tttccctgga ccgaatacag gaatcgtatt cggtcttttt ttaattgtat ttaaaatcaa
    27961 ttagttgcaa acgtctcccc gaaattcccc gaaatttact cgaatttctg tattccggtc
    28021 ttttttggtt atatcacaac caaaatacat ttaacaatcc atttacgtta aaatcagagc
    28081 agtaagtacg ttttttctct ctcatcaaga tacatttttg ttgtcttctc cgatgtgtgg
    28141 ccaagtagac gctgagcaaa ttcttctcca catatttctt tgtacaatcg tccagccagg
    28201 cttctgattt cgtgaaaagt tggtgggttt tcactgaaca gtgatacctg ttaattttct
    28261 ggctgcgaca aatttctttg tcaggccgtc cgggtgaatg ctgccgtcag ggctgttttt
    28321 tctaatcccg gcactgatta gataatcccc cctacttacc atgcggcact gttcaactac
    28381 tgcaccaagt cgtaaaccag cgacaggaag gctgagtgac agggggatag caatcatcat
    28441 tcctgtctta atttgcctga tgtggagacg atcatcataa atatcactga accgcatatt
    28501 cgttatgtct tcgcgacgtt gtcctgttac aagggctaag tccatagcta atgggaacca
    28561 tgccggaagt tgatctgctg cctccctgat gcagttgtat gtctttagtt tcagccgttc
    28621 tcttgtaact actattttcg gtgctcttgt tggcgttact ggattttgag atatacgtcc
    28681 ttcaacaatg gcttcgcgga acatatcaga taacacagaa cgcattgatc ctgccatcgt
    28741 gtttttccct ccttcaatct acaaatcaag aaactcggcg atatggcgag tggttatttc
    28801 tgtcagtaaa acccctccta atttttcttt tattgtctcc agttgattta cccgagtttt
    28861 ataagtattt ctggacgctt ttctccttat aagaatcgtt ttgtaacgtt caatccagcc
    28921 tgccatagta aatgagtcga cccctttaag cttttcaatt agggcagcag gagagtagtt
    28981 tttgtatata taatgatttg cttcaattgc ctgcgctact gcatctcttc ttgaaatttt
    29041 acctagtgta aattcttctt tcgtcagagg gttgcgccag taatatgctt tgtctctcct
    29101 tcgatatgtt aagtttctag gcaaattggg atcgtatttt ttccgctgca tgttttaact
    29161 tctccattaa cggactgtct ctcccttgtc gcccattagg ctgatggtgt gttatatcgg
    29221 tatcaacctt atttgggttg atatagaaag cctccggaac caccctgtaa ctcctcccgt
    29281 gtagttcagg tgcaggataa atgtttccat tccttgccca tcgtctcagc gttgatattg
    29341 atggtgggtt atccggatat ctgagttttc cccacgtttt gagtgtcaca agattcattg
    29401 ccatacctct cacgatatga ccgccagtaa atatacagaa tactggcggg tgtggttgat
    29461 ttttaataat ctgaggtgta ctggcaatag cggacactac catttgttct ttttttaagc
    29521 agccatctga tgatattttt ccctgaaggc tgccggggag atattcccca gacgagag
//
LOCUS       DBBBSB010000043        29269 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.043, whole genome shotgun sequence.
ACCESSION   DBBBSB010000043 DBBBSB010000000
VERSION     DBBBSB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29269
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.043"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACIUS0_001869"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACIUS0_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGB0219634.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACIUS0_001870"
     CDS             complement(1899..2636)
                     /locus_tag="ACIUS0_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219635.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACIUS0_001871"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACIUS0_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGB0219636.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3619..4104)
                     /locus_tag="ACIUS0_001872"
     CDS             complement(3619..4104)
                     /locus_tag="ACIUS0_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HGB0219637.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACIUS0_001873"
     CDS             complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACIUS0_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HGB0219638.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACIUS0_001874"
     CDS             complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACIUS0_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HGB0219639.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACIUS0_001875"
     CDS             complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACIUS0_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HGB0219640.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACIUS0_001876"
     CDS             complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACIUS0_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HGB0219641.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACIUS0_001877"
     CDS             complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACIUS0_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HGB0219642.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACIUS0_001878"
     CDS             complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACIUS0_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HGB0219643.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACIUS0_001879"
     CDS             complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACIUS0_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HGB0219644.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACIUS0_001880"
     CDS             complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACIUS0_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HGB0219645.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10978..11235)
                     /locus_tag="ACIUS0_001881"
     CDS             complement(10978..11235)
                     /locus_tag="ACIUS0_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HGB0219646.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACIUS0_001882"
     CDS             complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACIUS0_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HGB0219647.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACIUS0_001883"
     CDS             complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACIUS0_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HGB0219648.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13756..14061)
                     /locus_tag="ACIUS0_001884"
     CDS             complement(13756..14061)
                     /locus_tag="ACIUS0_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219649.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKNADISEQVTLVRLYLKVTFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACIUS0_001885"
     CDS             complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACIUS0_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HGB0219650.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACIUS0_001886"
     CDS             complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACIUS0_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HGB0219651.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACIUS0_001887"
     CDS             complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACIUS0_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HGB0219652.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACIUS0_001888"
     CDS             complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACIUS0_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HGB0219653.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(18848..19195)
                     /locus_tag="ACIUS0_001889"
     CDS             complement(18848..19195)
                     /locus_tag="ACIUS0_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219654.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(19223..19441)
                     /locus_tag="ACIUS0_001890"
     CDS             complement(19223..19441)
                     /locus_tag="ACIUS0_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219655.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(19521..19997)
                     /locus_tag="ACIUS0_001891"
     CDS             complement(19521..19997)
                     /locus_tag="ACIUS0_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219656.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(19990..20211)
                     /gene="traR"
                     /locus_tag="ACIUS0_001892"
     CDS             complement(19990..20211)
                     /gene="traR"
                     /locus_tag="ACIUS0_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HGB0219657.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(20346..20861)
                     /gene="traV"
                     /locus_tag="ACIUS0_001893"
     CDS             complement(20346..20861)
                     /gene="traV"
                     /locus_tag="ACIUS0_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HGB0219658.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(20858..21178)
                     /locus_tag="ACIUS0_001894"
     CDS             complement(20858..21178)
                     /locus_tag="ACIUS0_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HGB0219659.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(21165..21731)
                     /gene="traP"
                     /locus_tag="ACIUS0_001895"
     CDS             complement(21165..21731)
                     /gene="traP"
                     /locus_tag="ACIUS0_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HGB0219660.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPVLPGKMKCIRVVTADEYLKDVYTEDAS
                     LPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(21721..23172)
                     /gene="traB"
                     /locus_tag="ACIUS0_001896"
     CDS             complement(21721..23172)
                     /gene="traB"
                     /locus_tag="ACIUS0_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HGB0219661.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(23172..23900)
                     /gene="traK"
                     /locus_tag="ACIUS0_001897"
     CDS             complement(23172..23900)
                     /gene="traK"
                     /locus_tag="ACIUS0_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HGB0219662.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(23887..24453)
                     /gene="traE"
                     /locus_tag="ACIUS0_001898"
     CDS             complement(23887..24453)
                     /gene="traE"
                     /locus_tag="ACIUS0_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGB0219663.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(24475..24786)
                     /gene="traL"
                     /locus_tag="ACIUS0_001899"
     CDS             complement(24475..24786)
                     /gene="traL"
                     /locus_tag="ACIUS0_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGB0219664.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(24801..25160)
                     /gene="traA"
                     /locus_tag="ACIUS0_001900"
     CDS             complement(24801..25160)
                     /gene="traA"
                     /locus_tag="ACIUS0_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGB0219665.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(25193..25420)
                     /gene="traY"
                     /locus_tag="ACIUS0_001901"
     CDS             complement(25193..25420)
                     /gene="traY"
                     /locus_tag="ACIUS0_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HGB0219666.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
     gene            complement(25557..26228)
                     /gene="traJ"
                     /locus_tag="ACIUS0_001902"
     CDS             complement(25557..26228)
                     /gene="traJ"
                     /locus_tag="ACIUS0_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HGB0219667.1"
                     /translation="MYPTDPRQLNTERQIYLDKQFFVDVFSIPACVRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGEVVNVLWLFFCKNSNVIVDYCRGLRTNITNDRMLEFKNKSTEIQWKVFILYS
                     FGFCHESIASLLSITNGSSRNAISEVYKFFGIHSKHDLLMIFHTSRMHSLFFDELFFI
                     LKCAE"
     gene            complement(26422..26805)
                     /gene="traM"
                     /locus_tag="ACIUS0_001903"
     CDS             complement(26422..26805)
                     /gene="traM"
                     /locus_tag="ACIUS0_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGB0219668.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            27140..27730
                     /locus_tag="ACIUS0_001904"
     CDS             27140..27730
                     /locus_tag="ACIUS0_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGB0219669.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(28027..28848)
                     /locus_tag="ACIUS0_001905"
     CDS             complement(28027..28848)
                     /locus_tag="ACIUS0_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGB0219670.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(28959..29255)
                     /locus_tag="ACIUS0_001906"
     CDS             complement(28959..29255)
                     /locus_tag="ACIUS0_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219671.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         7367 a         7835 c         6576 g         7491 t
ORIGIN      
        1 agcctgagtc acatcttctc cggaggcaac ctccggttca aagaggctgg ccatctgacg
       61 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca gagagagttt
      181 gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata ccccgtcacg
      301 taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctcggggaa cggaaaaaag cacgggtgtt
      421 catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccacgca tccagtcacg gatggtgaag ggatcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag cgtgttcgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcatagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gggccacgac
     1501 agacgccaga acgaatgcag accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagtt ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaaa ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac ctgatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 tacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccta caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt tcattttcat caatgttcct tcatattgaa taaaacgtca
     3601 taatatacaa attaagattc actcatcata tcgttttgaa ctataaccat atgaattacc
     3661 accgatatcc acaccaatac ctgacatagg taaccctgtt gcaggattag tacgatgaaa
     3721 atccatatta tcaaaatcca gatgcaaatc ttcattagtt tgttcattta cctgagattg
     3781 catccattct cttacgctta cacaaccact aactcgcctt aattcgtata gtgtaccaat
     3841 gataaaaacc atcaggctta ataatggaga gagtaaggaa gatactggca tcgttatata
     3901 ccaaacgcct ttactaaact taaactcatc cgacacattc cgtttttttc gtatttgaga
     3961 gaacaaccat ataaacagtc cctgccatag tactatgaag aacagcataa aaattgtttc
     4021 ggcattcccc caactagcca gataacaggc gataaactgt atcaccgtta ccagagtaat
     4081 atgggttaaa catctcatta tcatctccca aaaacagcat tattgcttat acttatcctg
     4141 atattccttt gcccttttca tcagttcctg tggactgtca gccccgggca ttctttcctg
     4201 agccgacttt tcttcattat atttattatt ctgagataat gcctctgttt tatggtgatt
     4261 ctgcagttca gaatactgtc ctcttacaat attttcctcg ccacgtacgc tgttctgagt
     4321 atcacctata ttccctttat attctgtgac catattatca acctgatgtt ttacatcatt
     4381 acggatatta ctgtcctgcg ttctttgatc aataatggcc tgatgcccct gatggtcagc
     4441 aataatatcc tgactgccac cacccgaagg tacgctctcc attcctttac caatatcacc
     4501 acgggattcc cgccaggcat tatcaacacc aggctgtacc tgttcctgca caaaagacca
     4561 ggccatcgcc cgacgacgtt ctgcaatctc cggtgaactg gtgtttgtca gaatagcttc
     4621 tgcatcctgt ggcgcgtgtt tcattacata ctgtgcaaac tgttgcgaca ggtcttcact
     4681 catctgccca ctcatgcttt cagtacggga tgccatttca gcatattcat ggctgcgggt
     4741 catattcgtc gtgtactggt cgtaactctg cttcgctgaa ttcagggctg cagacaactg
     4801 atccacacgg gaatcagcat tattgtccgt atggctgccg gactcactga ccttgcgact
     4861 ggtaaaataa tcgctggcct ctttaaagtc ttgtgtagct ctggcatcga tatcatgacg
     4921 agcattatgg ctcgactgag tattactact tgccgtatgc gcatcattat cttcccaatc
     4981 aataccagcc cgaccaccac ctctaacgcc taatcctcca ccaacaccaa gaattttagg
     5041 tttaaccccc cattcagcat gagcatctcc atacatcccg gcagaagctc tcgtacttct
     5101 ggatgccagt tcctgagtcg cctgctcatt actgatatta tgcgccttcg catagctttc
     5161 cactgcactg cgcatacggc tggccatcat ggagtcctgt gcgctcatcg tgctgtcagc
     5221 accacctgtg acagaatcac tgctcccccg gttagaacca aactggctga gcgagttcca
     5281 ggcactggca atactgctgc tgaacccatg cagggcgctt tctgctctgt tcgacgcctc
     5341 ccgggccatt tcctgttgtg ccgctgcaat ctgacgcgtt gcattgatac cgaccggtaa
     5401 ccgggacatc gccccgctcg catccatcac catattaccg tcacgggtct gtgtggcagt
     5461 tgcgccactg ccggtctggt acatcatctg accaaacgac gtggtgctgt tggtgctcca
     5521 gctgaagccg ttcacgtttt ccgtctgcat gttgccgtag gagtaattac cgtcaaccac
     5581 actacctgcc gcactggcag tcgggctgat agcagaggaa gcgaaatggc tgtacacgct
     5641 ggaaaaacct gcgccaaggc cttttaccat catccatgac aacgggggta tcatgacaga
     5701 tatatatccg gcagtggacg ccaggtcgga gtatttcagc tgtatctgag agagttcaga
     5761 gagcacgacc ggtgcaccat tctgctttgc atagaatgtc atggcactgt tcagaatggc
     5821 atacagcaac ggccaggtct ggaaccacat cagagcaaac acatagcctc tcagaacaga
     5881 cagcgtcagt ctgttgaaga cagccgccag taccaggagc gggaagatac caatggtaat
     5941 gcccaccaga atggtctgca ccatcggcag gtttcgcatc agcacatgac caatggagac
     6001 atgtgccaga cgctgtttct ccatcgacga agtcgtggcc agactgacca ggctggcggt
     6061 atcaccgtta cgggctgcat tactggtgat accctctttc agggcattca tggtgacgtt
     6121 ctggcgcata atatgactgg cagactggct ggagccgtag aaataactgt aactgtcact
     6181 caccagttgc ctgaataaca ggtccgggtc cggtctgccg ccaaatatct gctgcacata
     6241 ataatgccag gtcttgcctc ccgtctttgt atcgagattc agcctgtctt tcagcgtgac
     6301 cgacgcatct ttacaggtta tgaaattatt attgctgtca taaacgcccc gtagcgggct
     6361 gggccgggaa aaaatcagcg tgtaggggtc agcggaggcc atcaggtctt ccagcgtgta
     6421 tttgtggttc aggtaaatat cccccagcac gcagttctgg acgtagtcct gaaacagatt
     6481 gatgatttcc ggattccggg acagaaaatc ggtgcttttc acaatcaggt tcgccccgaa
     6541 cagcatgcct gtcttgctgt aggtggcgct gtcaggctgg gtgaatatca tctcgtaact
     6601 ggccaccatt gcatgcccga tacgggtcgt cagtgaaagt ggcatcgcca gtccgacagg
     6661 cacattatcc acccggtgaa cctggaccag gtcactgtta tcaataatct gcaccgatgt
     6721 acggacatta accagcaggc tgataagcac aaacacggcc acccagccca gcaaatccat
     6781 cacgttgtgc cgctgtaccc acatcacggc caccgcgaga acagacaatg tgagcgcaat
     6841 tttttcgatg gaatcccacg tccgggtccc cataaaggcg gcaatggcgt tcaggttatt
     6901 gcgtaaccac tcaccaccgg caatcacata gacttcattc acagcgtgct ccctccgaag
     6961 tgatagttgt tctggtaacg gctgagcatg cgggcggaaa gctgctggcg catatagctc
     7021 atctgacggt caacaaccag caatgcatcc tgctggacct ttacctgccc ctggaaaacc
     7081 gcaatctgtt gctgtgcctg attcagattc tccagcacac tgtccagcac ggtctgtgga
     7141 tagttcccgg tggcaatcat ggcccgggcc tgctgaagca gttcctgaat gtactggagc
     7201 atgatgtcgt agccgatata gtccgtcagc tggtaaacca cgctgctgga tacaccgagc
     7261 atctgcgggt caataagata tttaaagacg gggacggtgg tgctggaaat gaagcctttt
     7321 tccctgtcat ccagtggagt atcactcacc gctttattct gaatactggt gagcagtttt
     7381 gtaatctgtg atttcagggc tttatcccgg gcgatggtca catttgcatc cgccaccacc
     7441 ttcagacact tgtccgaatc gttgcagtga taaacttttg ccgttccccc ctccataatc
     7501 gcccggagga tatcgcggtc agtggtacgc gccggcaggg gggtaatctc gctgtcttcc
     7561 ccaaaaatca gcgtaccggt gagcgtcata ataaattctt tcagttcttt gttaccatca
     7621 agcatcctgt ttttggacag cgagtcccag atgatattga tgttcttcag cacccgttcc
     7681 ttatctttgt cactggcctt gtcacgaaca gacgatgact gtcctccgac cgtgcagccc
     7741 tgacgggacg cggcccagtc agcaaaaata ttgctctcac cggcaatgtc ctgacagacc
     7801 ttctgctgtg acacctgagt ctgaggaaat aatcctccga caatcccctg cgccacctga
     7861 caggagctga ggttcatact gttaatgtcg cttgccattt tctgcaggaa gtcttttgcg
     7921 gttttgattt ccggcaccgt tgcctgcagg gcaaggtcaa aaaagtaacc ggcagcgttg
     7981 ctcataatct gcttaacaaa acgctgcaac tgatcgccat taataaaact gaacgagccg
     8041 agataggcgt cgatgccgcc acatccggca ttgatatccg gcagcgtcat ggaaatcagc
     8101 tggatatttt tgacctgggt gcgggcatac agggagccac cataggcata cccggcggcc
     8161 tgcccctgcc agacgcccgg ttgtgtggta ttggaagcaa atcccagctt attaaagaac
     8221 tgattcatgt cgctgttcac atccgctgac gctgcgggtg tgaccgtcag taaagcggca
     8281 caaagaacca gaagaggttt aatgcgaagc attattgcca ccctccctta atttcttctc
     8341 ccggcgcata cggcgtttca gccggaatat tctgatcccc ccgtttatta attcatttaa
     8401 agggttcctg acgtatacga taagaaatcc acctgcaaac gcccataacg tatacggaat
     8461 gacatacatc aggatgttcc acatataacg aaaattattt aattccgggt cagctttcca
     8521 cgaatcaata caggcatcaa aaatactatg cataatcacc cataaaaaag ttccggcagc
     8581 aacgaacaca aaaaacagat caaaatatat atcagaaacc ctgcgttcat aatcataatc
     8641 ccgtttagtt tttctgtttt ttagttccgc atcatttctt ttttctttca ttataagttt
     8701 cctctgatat ttccgatgat tcattcagac cgttcatgcg gctgtaatat atttcagaca
     8761 ccgaaatcca tcccacaatt gcccccacaa aaaacacaac agaaacagaa aaggtaagac
     8821 acagcgaaac gacaatttcc acagggctgg cgggataaaa aagcccctga cggataaagg
     8881 ggatattcag gctgacgatt attcccgtcg cgatgccgga tattccggca atgagtaaca
     8941 ccacctgctt atttcgtacc tttttcctct ccgtacatct gcagaacggt atccatccgc
     9001 gccataaaac tggcggcatc cgttgccccc tgcaaaagcg gtaatgcctc aagtgtgttg
     9061 acgttgacca ggaaggtagt cggtgtggcc accgggatat tcgggaagaa ggtctgcatc
     9121 acgtccggtg gcaccggtaa tgcttccgga aaggcggtat ccccctgacc atccagggtg
     9181 tagggaaaaa cggaaaagcc gtactgctgt gccagctgtt tcagtaccgg gtcaaactgg
     9241 tgacagtaag ggcaatgccc ctgcataaac agcaccacct tccagtcggc caggttaacc
     9301 tgcctgccgt tgctgaggcg gaaccagcgg ggagccggct tttctgttct ggctgatgtg
     9361 tctgctgccg gttgtgcagg ctgcacggcc ataccctgcg gattccagag tcgttcaatt
     9421 tcatcccggg tggaggcctg cacacctgtg gccatcatca acaaaatgac cggcagtaat
     9481 ttagtgagag acatgtccgc cctcctgctg cccttccatc atggcctgtt gcctggcttc
     9541 cagtgcctca cgctcttcca ggctgacacg tgcaatccat gcatccagca cctggtatgc
     9601 gccgaacagc acaaggatgg ccgcaatcaa ctccgccaga aaaaaagcca tccagggttt
     9661 gctgtaaaca agacgggcga cgatatgaaa ccagacaccc agggaaaata cccacattcc
     9721 cgtaatatca agccggggta aagagaatct gtatttactt atcatgttaa actccggtta
     9781 ttatttacat gagagtattc atgtctgtaa tattcgcgtt tgtgtgatat taatcagatc
     9841 aacctcatta ttttgtatgg tttttattac cgttttatat gacttagcct gtcataagcc
     9901 cccttataaa aaaccacatc actccggacc atgctgatga caacataata caaacaaaca
     9961 tccttctgaa actgtcaaac ttcgtgataa ccggtatggt actgactgcc attgaaacaa
    10021 ccggtacggc acactgattc ataaccgcac cacaaagaaa caagaaatta tctggcagca
    10081 tacatacact tatgacattt actacaaaga agacaaacga gctcagcaga taaactcctt
    10141 tattatttgg tcctgtaaag aaataaccaa caacccccac gataaacagg ctggcacctg
    10201 taaaaaaaca caaatcccct gtatgcatat ataacctttg gttaaaaatt cggtttaaaa
    10261 tcttcagaaa cgttcaggaa ctgttttgcc aggtcgtcct gtgaaataaa gccgtatgat
    10321 aacgggcgaa cactgccctg tttcgggtca accagcatca tggccgggaa atatttcaca
    10381 ccgaggcgct gcgcctgccc ctggtcagtc cgggaatccg gcaacagcgg attaatcacg
    10441 ccatccacgg aaacgggaat aacactcaga ccataggtat ccctgaagcc attaatgacc
    10501 tgcgccagtt gcccgtcgat ggggtcctgc ccccggtaaa agaacatgat gccgtagtgt
    10561 tcagccagtt tcgcaatggc ctgtcgctgc tgcgcctggt ctgccgccag ctggttccgt
    10621 accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcgggatg tgccagcatg
    10681 gcctttttag cactcatggt gaaaagcccg gcctgctgag tccagtaatt ctgcagccgg
    10741 aaatatttaa caaaattatc cacgccggga tacagaatgg cttcgtacag cgcccgcttt
    10801 gttgccgtct gcagcgcggc cagtttctgc ataatatccg gctcctgacg gggggctgca
    10861 ggtacaggtt tattttcttt ttccttcgga tttattttct cgttatacca ctgccagcct
    10921 gcatctttcc ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta
    10981 ttcatcttca ttcctttcat cattttttct ttcatcatct ttctgcaggc cagtcagggc
    11041 agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaggataa cgaaagcagc
    11101 ggcaagcatc ccgtctgccg tggtttcttt taccgtatcc cacgaccaga taattaccgg
    11161 ggaaatgaca atcgctgtaa tcgaaagccg gataagaaaa tcaacaagac gattagaagt
    11221 gaatataact ttcatcattt cccccggtta ttttccatac cttactgtcc tgcctgtttc
    11281 agctggtcag caatctgctc tttcactttc tgcgtcagaa cgccactgtc cgggattttc
    11341 tggttattca tcaggtcttc gtagaagtta gtgaaatcca gtctgtcgaa ctgaattttc
    11401 tgcagctcat caaccgtaat cccccgacag tcaggatgct tcgcactgcc aaacccgata
    11461 cgcaactgcc cgttacgccc ctgctgctgg actatctgcg ccagcttcga atcaaactgg
    11521 cagtagctgc gctttttctg cagacagaca cccagcactt tcttcgaaca gaactctccg
    11581 acactgacgg tcagtttgtt gtcctttgcc tttcccagcg ccttttcttc gctgttgcac
    11641 ttcgccagac ccacatcctg accccagcca ccatccttac agcagttact gaagccggct
    11701 gcggccttac ggcagaattt cgcctcaccc gtgaaagccc tgacatccac accattcagg
    11761 gcggcaacat ccttacctgc ggcggcaagc gcagccagct gtgatactgc ttcaccaaaa
    11821 tcactgcttt ttccgctctg tgccttatcg cattcaccgt caaggcagaa gacatcacca
    11881 ccacagacca tcacttttcc tgatgttctg gactcgcagg agtacgtggc gtactcatgc
    11941 agacaggttc cctcatcgga ataaaacgcg cactgacgtg aggccagcgt acaggccgga
    12001 ttatcgacat atttctgaca ggtgccgtta tctgcggact gagtgacata tttatcccgg
    12061 tatgcccagc atgcctgtgt gatgttccag ggtttacctt ccatcactcc ggttttcgta
    12121 ccacccggct ctgaacactc agtgcctgtc agcacgccat ccgctttatt aaacggacaa
    12181 ctctccaccc actccacccg gggaacccat tctttatcac ggaccttcat cctgagtttc
    12241 agcgtaaagg tggaagcacc actgacaagc gattcataga ctttcctgtc accattcccg
    12301 tgcgggaggc aattaccgtt agcagtacag ccactaccgg tcagaacctg cccctgtgtc
    12361 acctgcaaac ctgtcgcccc ggacaggggg taagtatccg actgcccgtt tgccaggccg
    12421 acattaaagg ttgtattcat gaaggtataa cgggagttca ggaagaaaaa agcggcgtat
    12481 acagacaatg atgcactttc cactgtaccg gttaccggag ccgtgacact gaacaccagt
    12541 ttcagcccgt tcatactgaa ccggaactgt gagtgcggta tcgtcacttc acgatattca
    12601 tccgtgtatt tccagtcccc ggtgatactg gcagtccgtg tgcagtactc ctccaccatc
    12661 gtgtcccgct cacaggtata gtttgtgaat tcactgcggt ttatctcctg cgcactgcac
    12721 tgctgtccgg tatttcccac aatgctgtca gcccggttca ccacatccct gccggtctga
    12781 atgaacggtg catccggtga cagaatgtct ttcggcttgt tcatgaagga ctctgtgatg
    12841 gtttttccgg tttcaccggt cgcccattcc gtggtgccgt catttttcag gccgccatcc
    12901 ccgccggcag tcacgccacc gtaatatttt gtctcgtccg gattcgcgtt atagctgggg
    12961 atactctcct gtggcttaaa gccctgaata ctgcctgtac cctgcccctg aatctgacgg
    13021 gcaaaatcag aaccggcccg gtaatcactg ttgctgtcag cctgtgccat gccggcaacc
    13081 agagccagta tcagaggtaa aatacgtttc atttcccgga atctcctttc cctgccagta
    13141 aatcatgagc cacctgccgg cagtcacccg tggcggccac tttctccagc gcctggccga
    13201 cccgcaggtt tccccggatg atgtcgtatc cctgactgca gaacaccacc agtgccggta
    13261 cactgcgaat gccgtactgt gaaaacagcg tcgggtcgat ctgaacacca tcagtcgcgc
    13321 cgtctttcac cagggacagc acggcttcag cggtggtctt caggtcattg ttcaccatgc
    13381 cccgcagtgt ggcgggtata ccgtagtgcc gggtttcgcc cagcatccgt ttcagcccct
    13441 cttcgggaat ggaaaacgac acaaaataca gggcaccctg ccggggtttc ccgtcctgcg
    13501 aagcctgctg tttacgaacc agctcatcca gaaaatggtt atctgaacgc tgaagggggt
    13561 tttccagcac ctgtttctcc gcccaggctt tcagctgatg gtcaggtttc tctcgcagtt
    13621 gtctgcttaa attttcctgc tgcttcagga actggcggtt ttcagttgtg ttcacatttt
    13681 ctgaagcatg aacagcaaga tgcatccctg ctgtcagagc aaaaagtgca aatattttcc
    13741 ggttcatgat atttctcact ttctgctatt tacaggcatg aactcatcag aaaaaagata
    13801 attgagaaaa acagccacaa acgcacatgt aaatgtcacc ttcagatata atctgacaag
    13861 tgtcacttgt tctgatatat ctgcgttctt ccattcatga ataatttcag cgagaaaact
    13921 gtcaggtgca aaataaaata atataacggg cactattaca agaccaaaag gaaataattt
    13981 taatgttctg cttatcagcc tgagcagtac agcacttatt ttcctgaggt gctgaatata
    14041 tttactgtcc attgttgcca ttttcatttc ctcttattca ttaaaatgat ttacaggaat
    14101 acacagttac gtttacgcca catcaggtag ccgaagtttt tctttgtgtt gggcggattt
    14161 tttcccgtct cccagcgcat cacactgcgc ccgaacggat ggcactgtcc gctgtccgga
    14221 tacatattca ccatctgata acgccagcgt tctttaggca ggattgggga cggatattca
    14281 ttacagacgg cgttattttt cccgatggtt tccataatca tgccctgacg gtgtagtttg
    14341 aacgccatgc gttcactgac cagcagggag gactgcaacg gactggactc attactcacc
    14401 cagccattga acgggtacat actgccctgc gaaccggcac accagaacag aacatcgaga
    14461 ggcatattaa aggcgctggc aatggcatct gctgcacagg ccccctgtgc tatcggattg
    14521 gcaaagatga cagcttccgg attgagaatg gtggtcaggc tgctgtccgt ccaggtgggg
    14581 tcaatttcag aaagataagc gatatccagg tcaccacctt ccagacagcc cagcgatgtg
    14641 atgatgttaa gccagtacgt cagcgggtat ttgtaccagt gaacgtgata gaacgccccg
    14701 ttcacctgct tctcatcctt tttcgccgtt ccctgtgccg ttttaccaaa agccggcagg
    14761 ctgaagccca ggttcaccat gcatcccggt gaccgggtga cgtccgtcag cgccatcggc
    14821 tcccagtaac caatggccag cccgatacgc ctgaacagcg gcggcggtgc cggacaaatc
    14881 tgaatgggca tcgacgggtt cgccgtgtcg gggaccttac cctgactgac tttgatactg
    14941 cccagcgaga gcgggaaaat acagctccag cagatatccg tgatcgggtt tacaaaacgc
    15001 ccctcacagg cagaatccgc agatgccgca gggacatgac cggcgagaag gaataacatc
    15061 agcagccaca ggcttcgctt cattttctgc cctcctctgc cggaataaat tccaccttca
    15121 ggaagcgatc gccgggaacg gcgctcaccc gtgccgggac ctgatcaatg ccgaggcgct
    15181 ggcagagcac accgttctgg tcaaagtaaa cacggctgtc cagagacttc tgcatctccg
    15241 ggatactgcc ctgcacgagg ataattttgc tctccagtgt gggcggcgtc tggcgtttca
    15301 tccaggccac ctgcgccgga tcatcgccat tgatgaaata cagcgtctgg ttaaaaggca
    15361 catactgcag cggattcatc acttcaccct gacgggcaaa cacccgccct tcgttatccc
    15421 ggatatccgc agccagtctg acggacgggt caaacaaccg gctgctgtat ttctccgtgc
    15481 gtccgatacc gggcacggca gggggccgca gactgttgcg gatcacccgc tccttaaagg
    15541 catccatttt ccggcccatc tcaccggact gctccagtgc cgtcaggcgc tgcatgatca
    15601 ccgtcagcat gtccggctcc tttaccggcc acagatcgcc ccaggtgcca agatcggcgg
    15661 cggccacact ctgcccccat atcagcaggg caatcagccc ccggcatctc atggttccgc
    15721 tctcattcgc cgggcaatat cctgctggat ttcccgggtg atatccggag ccccctgtac
    15781 cacggcaggc gacaccagga tgaccgcgtg gtgtttctgc tgccacgcct gcaggctggc
    15841 ttccagtgcc gtgttaaaac gcgctgaaag ggcttttgac tgtgcttctg acagttgttt
    15901 ctgactggcg ctgtcaaaaa acgcatccac ggtctgtttc atgttgaagg cggcagtcac
    15961 cggtgcgttc agtctgacaa tgccgtaact gacggctgcg ttcagtaaca ccattgccag
    16021 gcatcccggc accgtccacc accagtggct gcgtcggggg gctgtaacat ccgtcgtttt
    16081 ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt cttccagcca ggcttccagc
    16141 gaagccatct ccggccctga cttcttccac gccagctgcc acaccgcctc atgaatactc
    16201 agcccctctt tacgtttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg
    16261 gcgcggctta acgggtccac aaacaggcgg tgccaggagg aatggttttc cacctgcagc
    16321 aggaaggaac tgaaccactg gtctttggcc gcaccaaact taccaatcat gtcgcgctgc
    16381 agtggctgga actggtccgg atacagctgg ttgtattttg cgaactcctt cgcactctgt
    16441 ttgaggataa ttttgtagga ggagttaccc catgccgcac gggccgcact ggaggccttg
    16501 tcagagtcaa agtcgacgat gttctgtgtg atggtgatat aggcaccggt atgacggcgg
    16561 gcggtacggt aacctttctc aataaactca ccgaccttgc gatttttgaa gtccagcaga
    16621 cgccagcctt catcaataac gttcagtttc ttgaggttac gcggcgtgcg gtacatcctg
    16681 ttctcgatgt agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc
    16741 agtccgccca gctccagcac caccattttc gcatcatccc gcagggacgg ctcatcggag
    16801 ttaaaatact ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg
    16861 tccagacggc tgcggatggt cggtgacccg gcatactgct cgctgtcact ggcgtttttc
    16921 aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt tggccagcca ggaggccctg
    16981 accgcctgca gcagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc
    17041 actgacaact ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac
    17101 gggttaaaac gcagggtttc accgtccaga tatacgccac ccatgttctc acacagggac
    17161 ttgtagccat cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg
    17221 ataagtggct gtatcagccc ggttttaccg gccccggagg tgccacagac cgccatgttg
    17281 tagttggtgt tattcatccc ccggaagaaa atatcgataa acgccagctg gttacggtag
    17341 gtgggtgcca gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg
    17401 gcaacattaa agctctctgc gcgctgaacc actccggcct ctttcagctg tttaaacaac
    17461 cctttcccgg ccataaaggg cagacaggtc aggaaattgc gcatgtggtt aaagcgcggt
    17521 gaaatcagct caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagc
    17581 gccgtttcat tgttgtcctt gcagaatgct gtgatgttga ggaagtagga cacgacagaa
    17641 gactgaccgg agcccagacg ctgacgcagt tccccccact ccttcgcctc tttctccacg
    17701 gacggaaacc atttggcata ggaggtcttc gactttttct ccaggtccat gtacttcagg
    17761 ttggcttcgc tgtgggtttt cacctggtct tccaccacca gagtcagcgt caggatgaac
    17821 ggacaggaga tggacagttc cgggttcagc aggttgctgt agttgtcggc catattccac
    17881 aggaaggcga tttccgggtt acgggccaga tggaaattca ggatacgggc cgtgctgttc
    17941 ctgccgttct cacgcaggcc cagcgtcagg taatcagccc ggacattcag gtcaaaactg
    18001 tcctccacac actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac
    18061 agggagtccg ggttatggtt aatcatctcc ccgacaatat cgataaaggc ctgtgcatcc
    18121 accgtctgtg tggcgatact ggccccctgt aacgacgccc ggatgatttt caccaggttt
    18181 tccatttcca gaatgtcagc ccggcttttt ttcttcgagg gagaacagta cgagataaat
    18241 acccggtaat ggcgcagggt caggggcaga ttcatcccct ccggcagcgg aaactgtgtc
    18301 gctgccgctt tcatataata ggcccgggta atggcgttaa accgttcagc ctgttcacct
    18361 gaccaggaga actcacgcag cccgtactca atcctgtcac caaccagctg actggacatc
    18421 agatggatac acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc
    18481 tccacaatgg acttattcgc cccattgatg ggaatggctt ccagcataaa gcccatcgtg
    18541 gtgtcattca tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg
    18601 aactgcggga agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt
    18661 ttcagtgctg tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattactc
    18721 atatgagtat tccttagtgc gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata
    18781 tcacgcagtg ccattcctga aaccacagca caaataagag atggaggctt ttaacaggaa
    18841 accaggatta acgttccttt actgaatgag catcatttaa tgcctgaatt tgtttttcgc
    18901 gaatcttatt gcgctcattt tctcttacac gtttcacctc tgccatttcg tttctttttc
    18961 tgcattctga tgcgatttta tttgccttac caagtgttaa aggttcagac tgataattaa
    19021 gtgtctcctg aaatgcttct gctgcacagg gagtttcctg taaaatctct gtcagttgtt
    19081 ctggtgttgc aggttgttgc gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag
    19141 ccataacgcc aacaaatata aaagccagaa cagattttaa atatatcgac ttcatttcat
    19201 tttccttcga agcagtaagc actcattgcc caccaccatt tagtgtttcc atcacgctgt
    19261 tcaaaggttc atttgtttta acttcaatac actcccctgt atttccaaag cccctgccgc
    19321 catgtgcttc gattgtccga agaacctgca cgcatacgtc cgtatttggc atgggtgtaa
    19381 tattccagct tccgccaccg ttaatatttt ttatcagcac aagaaaaata aaggtatgca
    19441 ttctttatta tctccgcttg tatattaatc actgaatatc ccttcagtta acttatgagg
    19501 tcataaaaca ctgcagattc tcattcggat ttattccctt cagtaaactt ccgaatagta
    19561 tcctgttcac gctgacgggt ggcagactcc tgcattgcat ccactttctc ctgccagaga
    19621 cgccggatat cctgttcatc ccgaccagtc agttttttac cggacaacaa acggttaagc
    19681 agttcctgtc tcacatctgg tacgtcttca gtcagacgca taagccggag taattcctga
    19741 cgactgagta ccatgcgtga ttcctccggt acgctgtaat aattaaaaat acgactgaat
    19801 accccacaac agcaggcaac ggacaggcac agggatttca gatacaggcc agaaacacag
    19861 aatgtggcaa caccacaaag aatagctata actaaaaatc ccttacccag tgccatacac
    19921 aaagaaccct gttttctgga tatccgctca tgctcatcca gaataaaatc tattttttca
    19981 ttttctgatt tatgcatagt gtttcctctg cctttcctga tacgcctgac attcaacgca
    20041 caacgtcaca ccgggaaata ttttccgccg ggcttccgga ataggatttc cgcatgcttc
    20101 acagagataa acaggaatac catgaacatt tatttcctgg cgtatccggt ttattcctgt
    20161 catggtcagt tgttctgtca ctgaatatgc ttcatcggct tcatcactca cggaatattc
    20221 ctcccgacat gttctgacac gctaccgccc atcccttttc acgcacaggg cactcgtttt
    20281 acaggcggat tttgaataca gaaagcccgc cggcattgcc ggctgatata caacttcagg
    20341 gccagttaat taatacgtgg ttttccccac gcagacggtt taataacaaa aaacacgctg
    20401 gatggctgat gataaacatc ctggttatca atataaggcg ctatccataa tgctgccgtc
    20461 tgctcacccg ttcttagcgg acgaggaggt gttaccggcg taactgaact gaccggaaca
    20521 accgttttca cttcccgggc agcggtaaac agcgggcgtg gtgccagcag tttctgctcc
    20581 ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg tcgcggtgac ggtctgcacc
    20641 ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg atgccgcaac cggctttgct
    20701 tctgaggact gctccagttt tttggccttc tcattggcct gctccatcgt catacaggta
    20761 tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc ctgcgcagcc ggaaagtaat
    20821 aaggttccca gcagaggaat aaataaagaa atctgtttca ttgcccttct ccttttttat
    20881 tactccccac aacagcagaa attaactctg ctgtcagatt tgttttccct gcacagggat
    20941 gtccggagac cactgtattt cccggagaag aaaataagct catcgacatt cccttcttca
    21001 tcacaccgac agcagccata ttaaaattgt gcggcaatga ttcagaactg aacaccttcg
    21061 gagcaggaag aactattttt gtggtgcagt tactgagaac agcatgcata aaatcatctg
    21121 atacgttttt atccggcaca acgcgggctg taataacatt aatattacgc atgttcattt
    21181 ttatctccgt tgcttctgtt tgttcggtca tcagggaaat aaacatggca tttcctgatt
    21241 tttcccggca gggaagcgtc ttccgtataa acgtccttca gatattcatc tgcggttacc
    21301 acccggatac atttcatttt tccgggcagc accggatatt tcccggtgcc tgccgcaaac
    21361 gacatataca tcagtgccag cgttgcccag aatgcggtga ccagttgcat cagtgaccgg
    21421 tcagtctccc tgacataatc tgcagcatca gccgcgcggt aatggcagat atcctgtatt
    21481 tctgatctgc ctgaagaatc agaggaagaa aaccagcatt cactgaccgg gaactgtccg
    21541 gcaggtgctg tttgtcttac gaagttaatc tccttaacca gtaatttccc cggcgtggtg
    21601 ttgtccttcc ataaaacaaa cagagccatc agggccattg tggccagcgg ccagataacc
    21661 acaaacctga cgacccacag cagccagcga acgacaaacg ccacacggaa tgtcagtgca
    21721 tcacccgcca tttccgccct ccttgccggc atccccgccc agcgggttct tgtctccgga
    21781 cataaactgc tgcggattca gattgcccag ctgtttcagc atctggtcgg tgttaaaacc
    21841 gttcaggtgg ctttctgccg agggaggatt cggcaccggg ctttccgggt taccctcttc
    21901 cgctgtactc tgtgactgct ccagtgccat ttcctccacg gttttcagct ggaagccgtc
    21961 ctggaacacc acggtcactt cgttgcccgc accaatcggt atcaccgggt gatactgttc
    22021 ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct ttcgatgcgc cgccaccgat
    22081 acccattttc aggacatcac cagccccgta agcggctgtg gcacccagcc cgacagccgg
    22141 ttgggaggcg cgctccattc cctgaccgat accgtcaaca aatcccgcgc cccatgccca
    22201 gccgaggatt ttgccgttac gcatcaccac ttcgcccttg ataccgtttt taccccggaa
    22261 gctgacatgc cccttaatcg gcatatcaat agttttgccg tccttcaggc aactgatatt
    22321 gcgggtgcgt acaatggcac gttcactgga cacatccccc caggcttcca gccccacaaa
    22381 acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc accaggccgg tgatacgcaa
    22441 ctgcatcggc accgtggatt cattaccggt gactgaggca ttggcatccg ccccttcaat
    22501 cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc agcgatggtc cctgttttcc
    22561 ctcattacgg gtaaacacct tacgctgtat ccggttaggc accggcacag actggtacgt
    22621 cacctgtggt ggtggcgtga caccattccc gggataaaac gccgtcggcg gtggaacagc
    22681 aacgctgccc tgcggcggga aggatacggg ggtgtttcct ggctgtggtt cgccttccgg
    22741 gccgggcggt ggggcaggca tctgcggtac aggctcaccg gtcgccgtga cgggattagc
    22801 acccagggct tttacctgct ctgccagggc ggcattgtcc tgtcccagct tttcaatacg
    22861 acgctggtca tcaccgcgct gtttgttcag aacatccagc tcacgacgga tttcctcata
    22921 ctgcttctgc atctgcgctg ccgtcacctg catctctgtg gtggcatgct gacgcacctt
    22981 gtcatcaaag gtcgtatcca ccacaccggt catatccggc acaggctcct gttcagccac
    23041 ggtttcaccg ttaccggaca tgtccacatc agacagatac agtgccccac caatcgcgga
    23101 ggctgtaccg acaaccacaa tccccagcca caggtactgc ttgcgtttca caatggtatt
    23161 gatactggcc atcagttgcc ctccccgtca cggatgacat acacatccat gcgcgcacct
    23221 gccagtaact ggctggcagg ctggctgaac atcacggcac gggttcccgg ctgccagaaa
    23281 tcactttccc ggatattcag ggcagacagc gttttatttt ccacgacaaa gcggaccatc
    23341 ttcaggtgat tccccgtcca tacggaatct gccactgaag acagccccgc cggggcctgc
    23401 agggtttcct ttgtcacagg aacctgatac cagcctgcag gtaattttcc gccacggaca
    23461 gcctggctga tggtcacaag cagggattcg taaggcgtgg acgtttccca cgctccggct
    23521 tcttctccgg ttccgcgcag gtcactgacc agctgaatgg tacgccccgc gccttcacgg
    23581 ggaacagcct gaatggaaag gttcagacca cgctctgttt ccagaatgaa cgtaaagggc
    23641 tttttgttga cggtggccac caccacaccg ccggaggcgg tctgctcatt attggtcagg
    23701 gcaccatcca gactgttcac ggcgataatc cggtcaccgg gaacggtaaa cagattcggg
    23761 ctggtattgc tgatggcaat actggcctga ccaccgttca ccattggcac cacagtgggg
    23821 gccagcgtac cgtttgcggc catcacgctg caggaaaaaa acaggccgcc gaatattatt
    23881 gccgtattat tttttctcat cgtctgtttc cccgaaatta tccagccagg tcaccccgtt
    23941 ttcccgcttc agaataagga tgtaatgttt gatatcagta aaaggttcgg agttaccaat
    24001 ccatgttttc cgaattccac ggatttccac acgaccatac tgaggccaga cacggacact
    24061 ggtctggaaa aaagcagaat tcacgttatc ttttttaatg agtttcgctt cttcagccag
    24121 aataactttc atctggttct gtgcacccgg gcggatatat tgcagaagcg cctgatgtga
    24181 agcatcgacg atttccggtg agacattcag acggagggca ataaatgaca gcgccatctg
    24241 ctgcaaataa gaggcatcgg cactgttctg tgacacggca aagggcacat taaatcccat
    24301 tggggtcacg gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag
    24361 gttaacagag aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac
    24421 acgactggta cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt
    24481 aatccactgc cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt
    24541 tggcatatac cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt
    24601 tttaatcccg aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt
    24661 gataccccag ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca
    24721 gcggctctgg ttggtcagtg tttccgggaa acgatatttc ttaagtttat tctcgtctcc
    24781 cgacatcgtt ttatttcctg tcagaggcca acgacgctca tacccacagc aataaataca
    24841 gagatgatgg caaagccgac caggaacttg acgtttttgg tcatcatgta catgaccgca
    24901 ccgaccagca cctcagccag aacaacccat ttaacgatac tggagtcctt accgaaggtc
    24961 gccttaaccg tatcgttacc tttagccatc aggtcgcctt taccagcagc catagctaac
    25021 tcagggaaca acatcatcag aacagcaacc gggatcactg cacgaaccag gcgaagcata
    25081 ttcagacgag tgaatttaga aaaaaacgat ttcttttcaa cgggcgcaga agcaccctga
    25141 acacttaaaa cagtattcat aagttacctc tattgtggat agattaataa acctaaagat
    25201 ctttaattat cgaatcagtt tcctctgctc cctcctttat tgcatccaga ttataaaagt
    25261 caggaaaacg gttcagatga tcacgtaacc tgactagcac ctcattagtt tttgttctcc
    25321 cactacgttc ccgggcacct aagagcttgt gattagtagc atcgtccaat accagtaaaa
    25381 ctttattgcc cggagcaggc cttattatat ttctgctcaa gtctcctcct cagccactta
    25441 gataattaaa tataattata cgaatccata aaaatagaaa cgaaaataga gtaaattaca
    25501 ttaacaaaac aaggtgacat aatgtcacct tgtttttaac aaaaacgaaa catcaactat
    25561 tcagcacatt ttaaaataaa aaacaattca tcaaagaata aactatgcat cctgcttgta
    25621 tgaaaaatca ttaacaaatc atgttttgaa tgtataccga aaaacttata cacttctgaa
    25681 attgcattcc gggaagaacc attagtaata gacaaaagag atgctatcga ttcatgacaa
    25741 aaaccaaatg aataaagaat aaaaactttc cattggattt ccgttgattt gtttttaaat
    25801 tcaagcattc tgtcatttgt tatatttgta cgtaatcctc tacaataatc cactatcaca
    25861 ttagaatttt tgcagaaaaa aagccataaa acattaacca cctccccata tattaatggt
    25921 ataaattgta ccagccagag tttatcacct atagccacgc tctgtatttt attcatggac
    25981 gaaggcaaag acatagcatc cagttcttct cttaagaacg atgtagctac ttgtacagga
    26041 agggagtaaa accactcttt gatatcaaga ctacctataa attctttaga gaatttttca
    26101 ttgtagccga taaaatctcc attagtattt cgaacacaag cagggattga aaaaacatcg
    26161 acaaaaaact gtttatccaa atatatttga cgttcagtat taagttgtct aggatccgtc
    26221 ggatacatag gaacctcctc tcaaaggatt ctatggacag tcgatgcggg gaggtcgctc
    26281 tccctgcatc ggcgattttt aatatctgtt ttaacgtggc attaaccaca taattaatat
    26341 aacctttatg tggttaattg tcatcaaatt gaaccagatc aaaatcctgt tatttgaagt
    26401 aaatcaactt ttaattcttt tttattcctc atcatttttt ggaaaaaatc gttccatctc
    26461 agatgacacc ttctcacgaa tatcttcaac catattggca tattcaaact ttggattccc
    26521 ggagacatgg ggacttagag actcaatacc caaaattttc gctactgatg actgtgtttt
    26581 tacaacacat tcaagaagaa gtttattaaa ctctgtctga ttaaacgcag actctttacg
    26641 ctccatctga gcctcatata cacgaagccc cagttcaaga agcattgaag ctgttcccga
    26701 aacacttata tctttatctc ttgccccctc ctgacgacgc tgttcaacta tcgcatttac
    26761 tttatcatag acatcattac tgatatacaa aattactctg gccataagat aaaacctttc
    26821 attattaagc aatgaacttt tcactataaa tatgcatata gtgtttacaa gtaagaaaga
    26881 cactcctagc agcgcctcta ggatcatcct ataaaaaaat gcgatccggc gctaggggcg
    26941 tccctaatat atatcaatgt ttttcatgaa aattgtcagt actgatccta ataagagtcg
    27001 ctatagggtc gtaacaggat cgccaacgac tctctattta ataattcaga attattaaat
    27061 ataaatagcg tttgttaatt acatgattta aaacgtaaat cagcaaaaac ttgtttttgc
    27121 gtagtgtgtg gtgcttttgg tggtgagaac caccaacctg ttgagccttt ttgtggagtg
    27181 ggttaaatta tttacggata aagtcaccag aggtggaaaa atgaaaaaat ggatgttagc
    27241 aatctgcctg atgtttataa atgggatctg cgaagccgcc gattgctttg atcttgcagg
    27301 tcgggattac aaaatagacc cggatttact gagagcgata tcatggaaag aatcccgtta
    27361 ccgggttaat gccatcggta ttaatccggt aacgggatat ggcagcggac tgatgcaggt
    27421 agattcccag cattttaacg aactggcccg ctatggaatt aagccggaac atctgacaac
    27481 agatccctgt atgaacattt ataccggtgc ttattatctg gcaatagcct ttaaaaaatg
    27541 gggcgtctcc tgggaggccg ttggtgcata caatgccgga ttcaggaaga ccgaacgcca
    27601 gaaccagaga cgtcttgcct acgcatcaga ggtttaccgg atttatacct ggataaagag
    27661 cagtaaaggc atccgggttc cgaccacgaa gaaatcactt tcccaaatta acagtgtgca
    27721 gaaaaattaa tattgtatac attgtcaaat accggttatg ccgcgacagc ggcatggaag
    27781 gccacacaca aaaatatcat atgagtgata atgctgactg gccgaagccc cgcaaagcgg
    27841 gatcccatct tatatatatc cggtaactgc gcagcctcct gccagaccat ttttcgcggg
    27901 cacaatattt acagactacc gaaaagccag ttgatgaaat tgaggcatat cagacgacac
    27961 agcaatgccg caggatgata atataagtaa agccccgaaa aatttttcgg ggctttactt
    28021 atattatcat gacttccact ccctgaactg ttcggcaatc atccataatg ctttgttgat
    28081 tcggatatca ccgtcaatgc ctgtaatggc tcttgttctg gtatttttac cggaggcact
    28141 tcgccccgat aatccacctt ttatcatatt ctcctgaacc cgctgccagg ttgtccagag
    28201 atcgttctgt ttatcctccc aacggcgggg agtaattatt tgttctggcg tcactggcgt
    28261 tttattttca tcttcgtacc tggccatcaa tgcagctctg ccaaataaat gttgttcgtc
    28321 actgttaaga tgtatctctt tcattgtctc catattttca gtgactttat caaagacacc
    28381 gagcacttca tacgctccct caataacctg cccgacaata tcacctttat gtggaacgcg
    28441 gatttcgcca aaattattcc cacacaccag gccatttgtg cagacaaaac gaaaaattcc
    28501 tgggatcatc tgataactgg atgaaccatc atgtgaatta agcaggataa tttcaggaac
    28561 ttctttcccg ttaatatgcc cttccctgcg aagacgtaac atatgtttac tgtattcgcg
    28621 gcgtcccaaa tcacgaaccc gactctgaca ggcaaagaat ggctggaaac cttcatcacg
    28681 taacttattg atgatattga tggttgggat atatgtataa cgctcactcc gggattcatg
    28741 tttatcacca gaaaagacag atggaacaaa ctgcattaat tcgtcatccg ttaacgggcg
    28801 ctcacggcga atagaattat atcgaccaaa acgggaagct aatcgcataa cagtaactcc
    28861 tttatgaagg cataaaatta ataaaagttt tttcgcttaa tatcatcccc cctcggttat
    28921 cgttaacaaa aagacaacaa ttccgagggg aaggaccatc aaatcaggtc agttcgggac
    28981 tgaccaacct gccgagtgaa aagcagtaac agcgcgatca actcatcagc gttttttctg
    29041 acaagaggag tatgattctc caggctatcc agtaagtcat gcagctcatt attgacctgg
    29101 tggagcacct taaagagtgc accagaatcc cggcagctct gatatgcaag atttgcctga
    29161 acagcacgat tctgcatgat cccgatttgt gcgattgcgt ctttctgctg atcgcctgtt
    29221 ggctgataag tggtgttaaa agaaaatgcg ctcataattc ctcctgtat
//
LOCUS       DBBBSB010000044        29087 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.044, whole genome shotgun sequence.
ACCESSION   DBBBSB010000044 DBBBSB010000000
VERSION     DBBBSB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29087
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.044"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..156
                     /locus_tag="ACIUS0_001907"
     CDS             <1..156
                     /locus_tag="ACIUS0_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0219672.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /gene="uidR"
                     /locus_tag="ACIUS0_001908"
     CDS             385..975
                     /gene="uidR"
                     /locus_tag="ACIUS0_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGB0219673.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3066
                     /gene="uidA"
                     /locus_tag="ACIUS0_001909"
     CDS             1363..3066
                     /gene="uidA"
                     /locus_tag="ACIUS0_001909"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGB0219674.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="ACIUS0_001910"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="ACIUS0_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="ACIUS0_001911"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="ACIUS0_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGB0219675.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="ACIUS0_001912"
     CDS             complement(5627..7135)
                     /locus_tag="ACIUS0_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGB0219676.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACIUS0_001913"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="ACIUS0_001913"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGB0219677.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /gene="fumB"
                     /locus_tag="ACIUS0_001914"
     CDS             8610..10256
                     /gene="fumB"
                     /locus_tag="ACIUS0_001914"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGB0219678.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="ACIUS0_001915"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="ACIUS0_001915"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGB0219679.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACIUS0_001916"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="ACIUS0_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGB0219680.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACIUS0_001917"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="ACIUS0_001917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGB0219681.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="ACIUS0_001918"
     CDS             complement(14284..15603)
                     /locus_tag="ACIUS0_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HGB0219682.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACIUS0_001919"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="ACIUS0_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGB0219683.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="ACIUS0_001920"
     CDS             16700..17035
                     /locus_tag="ACIUS0_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGB0219684.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACIUS0_001921"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="ACIUS0_001921"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGB0219685.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="ACIUS0_001922"
     CDS             complement(17791..19173)
                     /locus_tag="ACIUS0_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGB0219686.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACIUS0_001923"
     CDS             complement(19359..20303)
                     /gene="ydgH"
                     /locus_tag="ACIUS0_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGB0219687.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="ACIUS0_001924"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="ACIUS0_001924"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGB0219688.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="ACIUS0_001925"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="ACIUS0_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGB0219689.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACIUS0_001926"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="ACIUS0_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGB0219690.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACIUS0_001927"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="ACIUS0_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGB0219691.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="ACIUS0_001928"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="ACIUS0_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGB0219692.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="ACIUS0_001929"
     CDS             complement(25948..26769)
                     /locus_tag="ACIUS0_001929"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGB0219693.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="ACIUS0_001930"
     CDS             complement(26869..26952)
                     /locus_tag="ACIUS0_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219694.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACIUS0_001931"
     CDS             complement(27045..27353)
                     /gene="asr"
                     /locus_tag="ACIUS0_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGB0219695.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27624..27728
                     /locus_tag="ACIUS0_001932"
     CDS             27624..27728
                     /locus_tag="ACIUS0_001932"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGB0219696.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27777..29030)
                     /locus_tag="ACIUS0_001933"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="ACIUS0_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         7468 a         6926 c         7422 g         7271 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa cactctcgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggtat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catagcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgtca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttccaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agtcgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccgcgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgcgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatc
//
LOCUS       DBBBSB010000045        29043 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.045, whole genome shotgun sequence.
ACCESSION   DBBBSB010000045 DBBBSB010000000
VERSION     DBBBSB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.045"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..90
                     /locus_tag="ACIUS0_001934"
     CDS             <1..90
                     /locus_tag="ACIUS0_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219697.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="ACIUS0_001935"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="ACIUS0_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGB0219698.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="ACIUS0_001936"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="ACIUS0_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGB0219699.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="ACIUS0_001937"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="ACIUS0_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGB0219700.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..6990
                     /gene="fdhF"
                     /locus_tag="ACIUS0_001938"
                     /pseudo
     CDS             4843..6990
                     /gene="fdhF"
                     /locus_tag="ACIUS0_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            7084..7773
                     /gene="yjcO"
                     /locus_tag="ACIUS0_001939"
     CDS             7084..7773
                     /gene="yjcO"
                     /locus_tag="ACIUS0_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGB0219701.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="ACIUS0_001940"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="ACIUS0_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGB0219702.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="ACIUS0_001941"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="ACIUS0_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGB0219703.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="ACIUS0_001942"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="ACIUS0_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGB0219704.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="ACIUS0_001943"
     CDS             complement(10475..12193)
                     /locus_tag="ACIUS0_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGB0219705.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="ACIUS0_001944"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="ACIUS0_001944"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGB0219706.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="ACIUS0_001945"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="ACIUS0_001945"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGB0219707.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="ACIUS0_001946"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="ACIUS0_001946"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGB0219708.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="ACIUS0_001947"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="ACIUS0_001947"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGB0219709.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="ACIUS0_001948"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="ACIUS0_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="ACIUS0_001949"
     CDS             18432..18746
                     /locus_tag="ACIUS0_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGB0219710.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="ACIUS0_001950"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="ACIUS0_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGB0219711.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="ACIUS0_001951"
     CDS             20570..21862
                     /locus_tag="ACIUS0_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGB0219712.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="ACIUS0_001952"
     CDS             complement(22016..23665)
                     /locus_tag="ACIUS0_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGB0219713.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="ACIUS0_001953"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="ACIUS0_001953"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGB0219714.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="ACIUS0_001954"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="ACIUS0_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGB0219715.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="ACIUS0_001955"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="ACIUS0_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGB0219716.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="ACIUS0_001956"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="ACIUS0_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="ACIUS0_001957"
     CDS             28753..29034
                     /locus_tag="ACIUS0_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGB0219717.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7352 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DBBBSB010000046        28915 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.046, whole genome shotgun sequence.
ACCESSION   DBBBSB010000046 DBBBSB010000000
VERSION     DBBBSB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.046"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACIUS0_001958"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACIUS0_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGB0219718.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACIUS0_001959"
     CDS             complement(2138..2584)
                     /locus_tag="ACIUS0_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGB0219719.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACIUS0_001960"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACIUS0_001960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGB0219720.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACIUS0_001961"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACIUS0_001961"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGB0219721.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACIUS0_001962"
     CDS             complement(5347..6009)
                     /locus_tag="ACIUS0_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGB0219722.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACIUS0_001963"
     CDS             6182..6595
                     /locus_tag="ACIUS0_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGB0219723.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACIUS0_001964"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACIUS0_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGB0219724.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACIUS0_001965"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACIUS0_001965"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGB0219725.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACIUS0_001966"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACIUS0_001966"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGB0219726.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACIUS0_001967"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACIUS0_001967"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGB0219727.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACIUS0_001968"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACIUS0_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGB0219728.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACIUS0_001969"
     tRNA            complement(9861..9948)
                     /locus_tag="ACIUS0_001969"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACIUS0_001970"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACIUS0_001970"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGB0219729.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACIUS0_001971"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACIUS0_001971"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGB0219730.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVELVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACIUS0_001972"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACIUS0_001972"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGB0219731.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACIUS0_001973"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACIUS0_001973"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGB0219732.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACIUS0_001974"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACIUS0_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGB0219733.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACIUS0_001975"
     CDS             14984..15841
                     /locus_tag="ACIUS0_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGB0219734.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACIUS0_001976"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACIUS0_001976"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGB0219735.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACIUS0_001977"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACIUS0_001977"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGB0219736.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACIUS0_001978"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACIUS0_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGB0219737.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACIUS0_001979"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACIUS0_001979"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGB0219738.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACIUS0_001980"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACIUS0_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACIUS0_001981"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACIUS0_001981"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGB0219739.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACIUS0_001982"
     CDS             complement(22884..24023)
                     /locus_tag="ACIUS0_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGB0219740.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACIUS0_001983"
     CDS             complement(24069..26165)
                     /locus_tag="ACIUS0_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGB0219741.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACIUS0_001984"
     CDS             complement(26165..26911)
                     /locus_tag="ACIUS0_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGB0219742.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACIUS0_001985"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACIUS0_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGB0219743.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACIUS0_001986"
     CDS             complement(27659..27964)
                     /locus_tag="ACIUS0_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219744.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACIUS0_001987"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACIUS0_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGB0219745.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7319 c         7600 g         6992 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagttggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DBBBSB010000047        28670 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.047, whole genome shotgun sequence.
ACCESSION   DBBBSB010000047 DBBBSB010000000
VERSION     DBBBSB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.047"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            164..649
                     /locus_tag="ACIUS0_001988"
     CDS             164..649
                     /locus_tag="ACIUS0_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219746.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..1032
                     /locus_tag="ACIUS0_001989"
     CDS             646..1032
                     /locus_tag="ACIUS0_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219747.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1057..3270
                     /locus_tag="ACIUS0_001990"
     CDS             1057..3270
                     /locus_tag="ACIUS0_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGB0219748.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3293..3738
                     /locus_tag="ACIUS0_001991"
                     /pseudo
     CDS             3293..3738
                     /locus_tag="ACIUS0_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3760..7318
                     /locus_tag="ACIUS0_001992"
                     /pseudo
     CDS             3760..7318
                     /locus_tag="ACIUS0_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            7538..7741
                     /locus_tag="ACIUS0_001993"
     CDS             7538..7741
                     /locus_tag="ACIUS0_001993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219749.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7759..8079
                     /locus_tag="ACIUS0_001994"
     CDS             7759..8079
                     /locus_tag="ACIUS0_001994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219750.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8145..8377)
                     /locus_tag="ACIUS0_001995"
                     /pseudo
     CDS             complement(8145..8377)
                     /locus_tag="ACIUS0_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACIUS0_001996"
     CDS             complement(8487..9257)
                     /gene="yafV"
                     /locus_tag="ACIUS0_001996"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGB0219751.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9438..9884
                     /gene="ivy"
                     /locus_tag="ACIUS0_001997"
     CDS             9438..9884
                     /gene="ivy"
                     /locus_tag="ACIUS0_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGB0219752.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACIUS0_001998"
     CDS             complement(9927..12371)
                     /gene="fadE"
                     /locus_tag="ACIUS0_001998"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGB0219753.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12611..13189
                     /gene="lpcA"
                     /locus_tag="ACIUS0_001999"
     CDS             12611..13189
                     /gene="lpcA"
                     /locus_tag="ACIUS0_001999"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGB0219754.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13395..14162
                     /locus_tag="ACIUS0_002000"
     CDS             13395..14162
                     /locus_tag="ACIUS0_002000"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGB0219755.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACIUS0_002001"
     CDS             complement(14133..14873)
                     /gene="dpaA"
                     /locus_tag="ACIUS0_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGB0219756.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15257..15778
                     /locus_tag="ACIUS0_002002"
                     /pseudo
     CDS             15257..15778
                     /locus_tag="ACIUS0_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15954..16451
                     /gene="rayT"
                     /locus_tag="ACIUS0_002003"
     CDS             15954..16451
                     /gene="rayT"
                     /locus_tag="ACIUS0_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HGB0219757.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16668..18380)
                     /locus_tag="ACIUS0_002004"
                     /pseudo
     CDS             complement(16668..18380)
                     /locus_tag="ACIUS0_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18352..19137
                     /gene="lafU"
                     /locus_tag="ACIUS0_002005"
     CDS             18352..19137
                     /gene="lafU"
                     /locus_tag="ACIUS0_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGB0219758.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19208..20263
                     /gene="dinB"
                     /locus_tag="ACIUS0_002006"
     CDS             19208..20263
                     /gene="dinB"
                     /locus_tag="ACIUS0_002006"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGB0219759.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20315..20608
                     /gene="yafN"
                     /locus_tag="ACIUS0_002007"
     CDS             20315..20608
                     /gene="yafN"
                     /locus_tag="ACIUS0_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HGB0219760.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20611..21009
                     /gene="yafO"
                     /locus_tag="ACIUS0_002008"
     CDS             20611..21009
                     /gene="yafO"
                     /locus_tag="ACIUS0_002008"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HGB0219761.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21019..21471
                     /locus_tag="ACIUS0_002009"
     CDS             21019..21471
                     /locus_tag="ACIUS0_002009"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0219762.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21717..22760
                     /locus_tag="ACIUS0_002010"
     CDS             21717..22760
                     /locus_tag="ACIUS0_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGB0219763.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALSIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22822..23436
                     /gene="prfH"
                     /locus_tag="ACIUS0_002011"
                     /pseudo
     CDS             22822..23436
                     /gene="prfH"
                     /locus_tag="ACIUS0_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACIUS0_002012"
     CDS             complement(23493..24950)
                     /gene="pepD"
                     /locus_tag="ACIUS0_002012"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGB0219764.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25212..25670
                     /gene="gpt"
                     /locus_tag="ACIUS0_002013"
     CDS             25212..25670
                     /gene="gpt"
                     /locus_tag="ACIUS0_002013"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGB0219765.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25762..27006
                     /gene="frsA"
                     /locus_tag="ACIUS0_002014"
     CDS             25762..27006
                     /gene="frsA"
                     /locus_tag="ACIUS0_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGB0219766.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27064..27465
                     /gene="crl"
                     /locus_tag="ACIUS0_002015"
     CDS             27064..27465
                     /gene="crl"
                     /locus_tag="ACIUS0_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGB0219767.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACIUS0_002016"
     CDS             complement(27504..28559)
                     /gene="phoE"
                     /locus_tag="ACIUS0_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGB0219768.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7084 a         7122 c         7606 g         6858 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagttgctc aatccaccaa atattatgaa gaaaatgata tacatgatgg aagtaatgac
      781 atctttttta tgaagaaata tgggccgcct gctcatgagt tcgtgaggcc acgatataaa
      841 tttgttggtg agtattatat cggcttgaat caaacttatc gcataacaga tcctgaattt
      901 caagatgttc ctattaagga aatgttctgg aatttgcagg gggatttgaa tctgacatgt
      961 tggtttcatt ataaagacgg gcaatggaga gtgattactt atattttttg gccaccaggg
     1021 gctagatttt aatccggtat taaaggagca aatatcatga gttttgtatc tacgggtaat
     1081 aaaccagttg gaaacggtgg acccgtcatc accacaccac caatagctgg cgaaagtggc
     1141 ggtatgagca caggttcagc tgtaaccgat gtatctggtg ccgccgagga gatggcagaa
     1201 caggctgcgg cggacctgtt tggtgcgctg ccggaaccgt caggtctggt taaagccgca
     1261 gtagctgctg cgcaggctgc cgctgcagca gcagggatct cggatatggc tggtgcagtt
     1321 caggatgcag cggcgtcgct tgcagccggt gctccggggg cgcataacgt cacagtaagt
     1381 ggtagtgcgg tgccgccgca gatgcttttg tttgcaggca tgaatggttc ggaagggctt
     1441 gggaatctgt tttcttacac agtacagctt aaaacgctgg acgccctgaa cctggggtac
     1501 gtttcccccg ctgctaacct gccgcttcag ctcatggtgg gcaaagacct gtgcgtcagt
     1561 atcgaactgg acggcggcgg aaaacggtat atcagcgggc tggtgacggc ggcgagggtg
     1621 gtcggccatg agagccgttc ggtaacgtat gaattacgca ttgagccatg gctgaagctg
     1681 ctgacccata ccagcgacta caaagcgttc cagaacaaaa ccgtggtgga tattctcgat
     1741 gaagtactgg atgaatattc ctttccggtg gaaaagcggc tggtggaaaa ttatccaccc
     1801 cgcgcctggc aggtgcagta tggtgaaacc gacttcgatt tcatccagcg cctgatgcag
     1861 gaatggggga tttactggtg gtttgagcac agcgagaaca gccacacgct ggtgctggtg
     1921 gatgccatca atggtcataa agcgtgccct gactcgccgc tggtggagtg gcaccaggaa
     1981 gggctgaaac tggataagga atttatccac accattacgg ccagtgagag gctgcgtact
     2041 ggaaagtggg tgatggacga ttttgatttt atgaagcctc gttcgctgct taaaagtacc
     2101 gtggccagtc cgcgtgatac cggtcatgct gaatacgagc attacgaatg gcctggtgat
     2161 tacttcacta cgggcgaagg cgagatgctg acgcgcattc gtatggaagc gcagcgcagc
     2221 ccaggcagcc gggcacatgg ggccggtcat atccgtaccc tcatgaccgg gtacaccttt
     2281 acgttgatga atcacccgac ggcagaaatt aatcaggaat atctgctggt acagaccact
     2341 ctgtttttac gggataatgc gcagcacagc ggtcaggatc agcattttac ctatgtcacc
     2401 acgtttgaac tgcaccccac cagcgaagtt taccgcccac aacgaaccct gagcaaaccc
     2461 cacaccaaag gcccgcagag cgccatcgtc accggcccgg cggggcagga aatctggacg
     2521 gataaatatg gccgggtgaa ggtgcagttt ggctgggacc gttacggaaa gaatgacgaa
     2581 aacagttcct gctggatccg cgtcagctat ccgtgggcgg gtaaaggatt cggcatgatc
     2641 cagatcccgc gtattggcca ggaagtactg gtggatttca aaaacggcga tccggatttg
     2701 cccatcatcg tggggcgcac ctataaccag gacaccatgc cgccgtgggg gctgccgggc
     2761 atggcgtcgc agagtgggat attcagccac tcattgcagg gagggccgac taacggaaat
     2821 atgctgcgct tcgatgataa aacgggtgcg gaagaggtga agttccatgc ggaaaaagac
     2881 ctcaatacca cggtaaaaaa caatgaaacc cacacggtca tggtcgatcg cataaaaacc
     2941 attgtcaaaa atgaaaccat cagcgtggga gaagaccgta atgccaccat cacaaaaaat
     3001 gatggtcttt ccgtcaaact ggctcaaacc atcaatgtcg gaacgacata tcgtctggat
     3061 gttggcgatc aatttaccct tcgttgcggc aatgcagcgc ttgtattaca caaggatggc
     3121 tcgattgagt tttgcggaaa acaactgtta ctgcacacca gcgatgttat gcaactcatt
     3181 ggcagtggca ttgatatgaa tccggatggc ggcacagcca tgactgcgga tgatattgcc
     3241 ccgttaccta cccccgacgt ttctgaataa aaaatataaa aggaattttt ctatgaccag
     3301 cctttaccgt attcaggagg gctgctttgc cctgcctgaa acttttcttg atcgcaccgt
     3361 caatatcttc gtcccatcgg gcaatgaaag ggcaactccc agtctgaata ttttccgcga
     3421 tacgctcagg ccagatgaaa atctgaccac ctatattgac cgccagatag ccttgatgaa
     3481 aaaaaaaacc taggccagca ccgggtactt tcgcggggaa cggcactggc gggaactggc
     3541 gaagctgcca tgacaggtga acagattctc gcaactcatc gttctggaaa aacagaagtg
     3601 taccagcgtc aggccgggtt tatcaccggg ccagcaaagg tactaatgtt gacgctgacc
     3661 actcagcgtc cttttgatga tcacaccgac cagctatgga ctgcatggtt gactagtttt
     3721 cagcctgcta aaagctaaaa aaaacaggga ggttttgtca tgtatgaagc cgcacgggtg
     3781 gatgacccca tctaccacac cagcgccctt gccgggtttt tgataggggc catcataggt
     3841 atcgccatta ttgccgtcgc cagctttgct ttttttacct gcggcttttt ggccgggctg
     3901 attttgagtt ttctggctga tcagatagct tccggagtcc tgcaactggg tgaggctatt
     3961 gggcgctcga tacattccac agtgggaaaa attctgaccg gttctgataa tgtcagcacc
     4021 aacagtcgcc cggccgcccg ggccgtactg agtacggtga tatgcgaaga ccattccccg
     4081 gaaatccgca ttgcccaagg ttccggcaac atctacatca acagccagct gcccgtaagg
     4141 atgaccacac tgagtgtgat gcagtgattg aggacggctc ccccaatgtc tttctcggcg
     4201 gcggtacgca gacggtactg gaaatcagcc cggaaatccc tgactggctg cgtcaggtgg
     4261 tggatgtgct gtatgtggtc gccggaatgc ttggcgggct ggctggcgcc tggcgtcagg
     4321 cggcaaagat ggggtcgaaa tttggtacaa aatgtgcggc aaaatttatt ggtggcgaaa
     4381 tggtcgggat ggccatctct gacacagtca tgggattgtt cagcaatccg gtagatgtca
     4441 ccactgggca aaaaattttg ctgccggaga cggattttac cctgccagga cgcctgcctg
     4501 tcacctgttc gcgcttttac gccagccacc tggaaactga aggattgctg ggcagaggat
     4561 ggcggctgaa ctgggagata aacctgcgtg aggatgaaac atatatcacc ttcatcggtg
     4621 tacagggtcg ggaactgagt tatccgaaag agatgctgat accgggccac cagatattcg
     4681 acccggaaga gcagttttac ctgagccgtc tgcatgacgg atgctatgtg ctgcattaca
     4741 ccgactgcag ctattacgtg tttgatgaat ttgatgacca cggcgtcgcg ccgttacttt
     4801 ttatggagac gccgtaccgg cagcgtattg ctttcgggcg tgagaacggc aggctggtgc
     4861 gggtggcctc cagcagcgga caccatctgt tattgcaccg cacgatgacg caagcagggg
     4921 aacgcctgtc gcatattgaa cttttgaagg gcgggcgtcc cggtaacctg gtggaatatc
     4981 gttacgacga taacgggcaa ctgactggcg tggtgaaccg ggctggtgtg acggtgcgcc
     5041 agtttgccta tgaaaatggc ctgatgacag agcatcgcaa cgcgacgggc tttacctgta
     5101 cctaccactg ggaagagatt gaaggtttcc cgcgcgtggt ggaacacacg accagcgacg
     5161 gcgaggatta ccggttccac tatgattttg ctggcgggca gacggtggta accggcaggc
     5221 cggagcagaa gtggcagtgg tggtttgatg aagagacgta tgtgactgcg caccggacgc
     5281 cgggcggcgg aatgtaccgc ttcacgtaca acgaaaacca ttttccggta gcggtggaac
     5341 tccccggcga acgcagggta acgctggaat acgacaccct gagccgggtg gtgaaagaga
     5401 cggacccggc aggccgggtg acacagacgc agtggaacgg cagttttgct gaaatcacgc
     5461 gccgggcgct ggatgacgac cacgtatgga aggcggatta caacgaacac ggccaggtga
     5521 tacgggagac ggacccggaa gggcgcgtca cgcggtacgg gtatgatgat caggggctgc
     5581 cggagacggt ctgccacccc ggtaaacagc aggaccgtta cacctggaac gcgctggggt
     5641 tactgagcag ccaccgtcgc atcacgggca gtgtgcagtc ctggcagtat acgcagcgcg
     5701 ggatgctggc gcgccatacg gatgaagaga aacgggagac gcgctggcag tacaccccgg
     5761 aagggctggt tgccagtctg agcaacggca acggggctca gtaccggttc agctacgacg
     5821 gcgacggacg gctgacggga gaacagcgcc cggacggcct gataaggatg tttgcgctga
     5881 atgcggacgg gtttccggtc atcatcccga cgcagggcac agagggcggg gtgcgcaatg
     5941 aacagcagga gcgtgatgcg ctgggccgcc tgctgcgcag tgacacgcag cacagtacac
     6001 ggaccttcag ctacaaccgt cttgaccaga taacggaagt gacgctcacc cccacggaag
     6061 aaggggaacg gctgcaccac atgcaggcgg acacggtgcg ttttgcgtat gaccgcagcg
     6121 ggtggctgac ggcagagcac agcgtgcacg gcagtatcaa ataccggcgc gatgcgctgg
     6181 gcaatcccac cgatatcacg ttgccggacg ggcagcatct cagtcatctt tactacggca
     6241 gcgggcatct gctccagacg gcactggacg gtatcacggt cagtgaatat gagcgtgaca
     6301 gcctgcaccg tcaggtgata cgcacgcagg ggaaactggc gacgttcagc ggctacaacg
     6361 ccgacaaccg gttaagctgg cagcgcagcc tgccaggtgg cagccagaat ccacagcagg
     6421 cggtactccc ccggcgcaga acgacagcgt gacggcgcgg gactatatct ggaacgcaga
     6481 cggtgaagtc ggcggcatca atgacaaact gcgcggttgc ctggtgttca gctatgaccg
     6541 cagcggctgg ctgaccagcc gcacggggca gatgtatgac catgaccatt attattacga
     6601 caaagcgggc aacctgctga cggatgagta tcagggagcg gtgatggata accgcctgcc
     6661 gggttacggg cgtgaccgct accgctataa cgaatggggc gaactgacag agcgcaggga
     6721 ccagcagctg gagtggaacg cgcagggcca gttaacgcgg gtcatcagca gcaacagcga
     6781 gacgcggtat cagtacgatg cgctgggcag gcgcatcagc aaggcgacca gcaatctcca
     6841 taccgaccgg ggagaacgaa gccggacaac gtttgtgtgg gaagggttcc ggctgttaca
     6901 ggagacaacg tggcagggaa agcggacata cctgtacgat gcggaacagc cgtacacgcc
     6961 ggtggctgcc atcacggggc gcggagagag ccagaaaatc tggtattacc acacggacct
     7021 gacgggcacg gtacacgagg taaccgcgcc ggacgggacg ctggtgtggg cgggctacca
     7081 ggcgggattt ggtgaaaacc ggggcgacat cagcaacagc ggggcgtact ttgagcagcc
     7141 gcagcggctg ccggggcagt actttgacga agaaacaggg ctgcattaca atctgttcag
     7201 gtattatgcg ccggagtgcg ggaggttcgt aagccaggac ccgataggat taaacggtgg
     7261 gttgaactta tatgcttatg cgcctaatcc gttaggttgg atcgatccgc taggactgaa
     7321 ttctttagga atagattcaa ataatatatt tagaggagat ggtaactata aaggtggcgg
     7381 aattggacag ccaaagagtg gcacaatatc agcacaggac attattgatc atctctaaga
     7441 aaaaggtgat gggcgtttaa catcttttag tactaaaaaa acatctagtt ccaactctcg
     7501 tggtgcagat tttttctcaa aaaaacatgt cttcaaagtg gcagtttctg atctcgatga
     7561 tttagttaaa aataatcaga tcactgtttt aacacaagaa aaagtaaaag caatatttgg
     7621 tttagaatca agcaagaaaa ttagaaaaga acttggtaat attcttcaga atatggctag
     7681 gaataatgag gtgttagttc atgggcaaat tcttggaaat aaagttagcc gatgtaatta
     7741 gttagtggag aggaagttgt ggacgaaatt agaataagaa ttgctggtaa tacggctgcg
     7801 ccttgtttcc atgtgctaaa aaataaggga tataaagtaa caatggaaac tcatgcatta
     7861 tctgacgatt gtttagattg tgtatatgat tggtatgcgg aaaaaaatgg acaatttttt
     7921 agtgcaacga gtccagaaga gttacttgga ttagttgcaa tgtgggaagt tcgtggtaat
     7981 gactggagag ctaatttgaa tgagataaat acttatcaaa aagccataga agaatccccg
     8041 ttttttgata aaaacggcaa tgaaatagag tctgactaaa ttttattgat atagcaacga
     8101 taataaccgg aaatttcttc cagatctttc cggcttttta ttatttaact ttcgttcagc
     8161 acactaaaca tcccgtctcg catcccctta actggctgtt taacctgata aagttccctg
     8221 cgcagttcct gtacttcgtt gttatcccaa gcaggatcaa acatccctcc gagatcttca
     8281 cggtaacgcc cgtgccgatc tcaaatcccg cttttctcag ccatttggcg ctgattttga
     8341 tcaccggcgt gtcgctgcgg ttgggcagat aacccacggt acagcgacgc tgtgttttgg
     8401 aaatggtgac ttctggcgta atattgtgtt taagttaggg gctttcgtaa tctagcccag
     8461 cccccggccc taaacacaac ccccactcac cacaacctaa actcatccgc atcccgccat
     8521 gccggaaact tttctctata ttcccgcaat gccaccatcg acagctccgc atcaatacgc
     8581 gttgccttat gcgcgtcggc ggtagcgata atctcgcctt gcggattaat cacccggctg
     8641 tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac
     8701 gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagct
     8761 ggccagttgg cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacactgga
     8821 aaacgtaagt cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact
     8881 cgcgcattgc ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta
     8941 tcataaaaat gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac
     9001 tccgtttgta atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc
     9061 atccagttca ctacgtcatc ttgtgcaagc gacgaagctg ccgcttccat ggcaaagccg
     9121 ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga
     9181 tcaaaatggc gcaggttggc aggaccatcc acccacacca acggttgctg caaaagcgta
     9241 atcttcaaac taggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac
     9301 cttatcaggc agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata
     9361 tctgatttgc aaattatcgt gttatcgcta ggctttagga ggttaataac atgggcagga
     9421 taagctcggg aggaatgatg tttaaggcaa taacgacagt cgccgcactg gtcatcgcca
     9481 ccagtgcaat ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca
     9541 aagctgcgtt taatcagatg gtgcaagggc ataagctacc tgcatgggtg atgaaaggcg
     9601 gtacttatac tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg
     9661 cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta
     9721 atcagatgac ggggctgttc tcggctattg atgagaaaac gtcgcaagag aaactcacct
     9781 ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga
     9841 ccggcagcct ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa
     9901 cggagccttt cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc
     9961 aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc
    10021 agacggcttt cttccgcttt caccagaata gcggcttcat ctttatcaat cagccccttc
    10081 gtcagcgcgt tgtgcgccag ttcatccaga cgggtaaatg gcagattttt acccagctct
    10141 ttacagatcc gctgatgaat cgggtcggcg gcaatcacat ccaccaacgc ctcttccagt
    10201 aaaccaaccg ggttatgctc gcttggcgtc aggtactgac cgcgaccaat acgtgaacga
    10261 gtggcgttcg gcacttgtaa aatcttcgcc attttatgat ccagcttgtc agacggtgcc
    10321 agataatgac gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc
    10381 gggaagtttt gcagtaaatt gtccattgcc tgttctgcct gatacagcgc atcctgtacg
    10441 ccccagtgca ccagcggcag atcggcttca ttacggcctt cgtcgtcata acgcttcaga
    10501 acggcagagg cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatacgctca
    10561 cgacgtttca ggctgccgcc cagcagtgcc atcgagacat cagaaagcag ggcgaggttg
    10621 gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg
    10681 ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga
    10741 ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgccgcttcc
    10801 atctcttcca gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg
    10861 cgggtcagaa tgttagcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc
    10921 gccaggaagt tgctttgccc gagcataatg cctttaccgc cggtaatatc catcgcatca
    10981 ataatcgact gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca
    11041 ggtttttcgc cgagcataat gccgtaggta atcagcgatg ccgcagcatc cattacataa
    11101 gcgttcccgg caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt
    11161 ttgaactgac ggcgaatgtg agaatacgcg cccgttgcca gcgctaccga tttcacgccg
    11221 ccggttgagt tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc
    11281 cgccagcctt gcccggccat tttcggcccg ccgatgatgt aatcgatcgg cacgaagaca
    11341 tctttaccgc gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca
    11401 atttccacgc ccggcgtggt ggttgggatc agcgcacagg taatgcctaa atcttctgca
    11461 ccgccgagta atttttccgg gtcggagagt ttaaacgcca gcccaagcac ggtcgcaatc
    11521 ggtgccagcg taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc
    11581 tgccattcgc ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct
    11641 tccgggctgg tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag
    11701 tgatctttct gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt
    11761 ggcacgccga cggtaatcgc caggatcccg ctcacgccag agagtttttg cagcacacga
    11821 gactgggcat aagctgagaa ctccagtccg ccgtactctt ttttgataat catcgcgaag
    11881 aagcgatgct ctttaaggta cgcccacaac tccggtggca gatccgccag ctcatgagta
    11941 atctggaaat cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc
    12001 tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc
    12061 tggaacaagt cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc
    12121 gacatcggcg gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta
    12181 cgcataggcg caaagttaaa tggcacgagg ataatggtca gaggcaccag tacccacgcc
    12241 gaccacagac cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata
    12301 aataagctca cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc
    12361 aaaatcatca taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg
    12421 gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca
    12481 cattgttgct gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg
    12541 tacttctcgc ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa
    12601 ggatatcctc atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct
    12661 ggctaacttt ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt
    12721 agcagacagc tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg
    12781 cgacgctatg cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta
    12841 cccggcgatt gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt
    12901 caatgatatt ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg
    12961 gatctccacc tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa
    13021 ggggatgaaa gtgatcaccc tgaccggtaa agacggcggt aaaatggctg gcacggcgga
    13081 tatcgaaatt cgcgttccgc actttggtta tgccgaccgc attcaggaga ttcacattaa
    13141 agtgatccat atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag
    13201 gccggataag gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg
    13261 acgcgtctta tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg
    13321 ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg ggtccatgcg ctcccgctgt
    13381 tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct
    13441 gctttagttt caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct
    13501 ggggcattac cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct
    13561 ttaattcccc catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg
    13621 ctcatattcg ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc
    13681 gcgagttatg ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat
    13741 cactggaaac cggcaacttc cgcccggtcg gtgaaaccga cagcgaaaaa gccttttgct
    13801 ggctgctgca taaattaacg cagcgttacc cgcgcacgcc gggcaacatg gcggcagtgt
    13861 ttaaatatat cgcctcactg gcggatgaac tgcgggcgaa gggcgttttc aacatgctgc
    13921 tttcggacgg gcgctatgta atggcgtatt gctcgactaa tttacactgg atcacccgcc
    13981 gcgcgccgtt tggcgtggca acgttgctgg atcaggatgt ggaaatcgac ttcagctcgc
    14041 agaccacacc gaatgatgtg gtcacggtga ttgcgacaca gccgctgacg ggcaatgaaa
    14101 cctggcaaaa aattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt
    14161 gatgccagtt gtggctgcac cacttcgtgg cttaacggct tgctgaccac gtaacggcca
    14221 ttggccacag aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc
    14281 agctgctccc agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc
    14341 atgcggaatg ggtaaatact cacctgcacg ctcggctgac caaacaccag cgcaccagta
    14401 acgaactgga agatctcatc aataccctga ttggtcattg cgtagcagcc gatggataca
    14461 caatcgccgt ggatcatcag gtatttccct tcataaccat gcgcacggtc ataagcattg
    14521 gggaatccaa tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg
    14581 acgctataaa acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg
    14641 ccggaatatt tacagatttt ataactgtcg agcagttgat attgctcgcc cattttgacg
    14701 taaagatcga gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac
    14761 tgctgcttat actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca
    14821 aacgaaacgc acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt
    14881 attccttgat aaaacggtta cacacgccag gacggcaaaa tggatcccaa atcggaatag
    14941 tctggatttg gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgctg
    15001 ggtttacaaa tttagcacag gggcagtttt caatagtccg aaaagcgcat gtatcattta
    15061 tttcctaata ttatgaaatc tcaatagtaa tatgcaaata agcaattaat aatcccgttt
    15121 actcctccgg tcttttaaaa ttctcacctt cattcagccg gtttatgtct ttcatgtctt
    15181 cgttcttgct cggcagattt ttgcatccag gcgtgttttc gttatgcgtt ttgctccctt
    15241 tactcgccag cgccaccaca accgggagca gataaaacag catccagaat ggtttcctgc
    15301 gccgctcaag gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc actttttaag
    15361 cagcgatcac ctgcataaca tcaccgaagt ggcgattcac cgcctggagc agcagcttgg
    15421 caagccttac gtctggggcg gcacgcggcc cgatcaaggc ttcgattgta gtgggttagt
    15481 tttttatgcc tacaacaaga tccttgaggc gaaactcccg cgcacggcca atgagatgta
    15541 ccactaccgt cgggcaacga ttgtggcgaa taacgacctg cgccggggag atttgctatt
    15601 tttccatatc cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca
    15661 gtttattgag tcactgcgca ccggggaaac cattcgggta agccggttag ccgaaccttt
    15721 ctggcaggac cattttttag gcgcgcgcag gattttgacg gaagagatga ttttgtaggc
    15781 cggataaggc gtttacgccg catccggcgg ttgtgcacgg atgcctgatg cgacgctgac
    15841 gcgtcttatc atgcctacaa atctgtgcgt ttttgcgagc cgctttcccg atataacaca
    15901 gcgtcattgt cggcctttcc ttttcctctt accgttgaga aaaaggagtc gctatgtcgg
    15961 aatatcgtcg ttattacatc aaggggggaa catggttttt cacggtaaat ttacgcaatc
    16021 gtcgaagcca tcttctgacc acccagttcc agacgctccg taacgccatt attaacgtta
    16081 agcgagacag accttttgaa attaacgcct gggtcgtttt gccggagcat atgcattgca
    16141 tatggacatt acccgaaagc gatgatgatt tttcctctcg ctggcgggag attaaaaagc
    16201 agttcaccca tgcttgcgga ttgaaaaata tctggcaacc gcgtttttgg gagcatgcca
    16261 ttcgcaatac aaaagattat cgccatcatg ttgattatat ttatataaat ccagagaaac
    16321 atggctgggt aaagcaggtg agtgattggc cattctcaac gttccatcgc gatgtcgcgc
    16381 gagggttata tcccatcgat tgggcgggag acataacgga tttaagtgcc ggggagcgta
    16441 tcattttata actgtgcgct ggtgccggat gcgcttacgc ttatccggcc tacaaatcgt
    16501 gctttaacgg taggccggat aaggcgttca cgccgcatcc ggcagttatg cgcagatgcc
    16561 tgatgcgacg ctggcgcgtc ttatcatgcc taaaaacttg tgcctgaacg gtaggccgga
    16621 taaggcgttt acgccgcatc cggcaattgt gcaccgatgc cggattctta catcaacgcc
    16681 cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac atgcagcccc
    16741 ggcgcaaaca accttgcata acgcggcagc aaagggcgta gctgcggcgg caccagcagc
    16801 accggatctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac
    16861 tggttgagca tattcgggtc aaccggcacg ctgtcgagca tcactttgcc ggcctgttgc
    16921 gcctgattca ctacgttggt cagcagattt tccagctcat tattcagcgt atacaccgtc
    16981 agctcctgct tgcgaacgaa cggatgagta atgctgcgcc gcagcgccag gcgcacatcg
    17041 gccgccagca gaatatgatc tttagtcacc gtgctactgg cgaccagcac ggtggcgatg
    17101 gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaat
    17161 tggctgtaat tgagcgccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa
    17221 cggttatgca actgcgtgat gtcatcatag ttaaacaaat caggaatata gctgcgcaca
    17281 atcttgttca catgggtggc aatcacactg gcgctgtcga tcacctgata ccccatattc
    17341 agagccttcg ctttctgcgc aggctggatc caggtgaccg gtatcccgta cgccgggtcg
    17401 ttccccagca cgccgtcaat ctcgccgtag gtttcgctgg agggcagcgc catcagttta
    17461 tccgccggaa tatccgcttc atcagcctca atgccgttga tgaaaatagc gtactggctg
    17521 ggcttgaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttaccgtcg
    17581 gaaatcacct gccgaacgcc gcgaatccgc tgggtgagcg ggttgccctg cgctttgtcc
    17641 accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatagtt
    17701 tcccaactga cctgttgttc gctggtttca gtgatggtgc gggtcagcgt ttcgaggctt
    17761 ttctcttccg cctccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc
    17821 agggcgctga acagcaaaaa cggcagatgc ggcatccccg gcaccaccgc cagcacgaac
    17881 atgatcccgg tggcggtgta gagcaccgac gggcttgcca gcaactggtg gcgcacgtca
    17941 tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagc
    18001 agcagggaag ggatctgcgc caccaggccg tcgccgatgg tcatcagcac gtattgctgg
    18061 aaggcggcgt cggcgctcag gttgtatttg aagatgccga tacagacgcc gccgctcagg
    18121 ttgatcgcca gaatcatcat cccggcgatg gcgtctccgc gtacaaactt cgatgccccg
    18181 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgccgggt ttgcgcctgc
    18241 gcctggttga tcaatccggc gttaagatcg gcgtcaatcg ccatctgttt gccgggcatc
    18301 gcgtccaggg taaagcgggc agaaacctcg gaaatacgct cggcctcttt ggtgacgacg
    18361 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
    18421 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
    18481 catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgat
    18541 gtgaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagttggca
    18601 accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
    18661 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
    18721 ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc gccagtgttc
    18781 gactcgctct ataacaaaat tattattacc gggcataccg atgcgatggc ctacaaaaac
    18841 aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg
    18901 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
    18961 ctgctggacg cgaagaatcc gcaaagcgcg ggcaatcggc gtattgagat tatggtgctg
    19021 accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg
    19081 cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat gcgctaaatg
    19141 ctgaatcttt acgcatttct caaaacctga aatcactgta tactttacca gtgttgagag
    19201 gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat
    19261 gcgcgacaat cccgctctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg
    19321 gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc
    19381 tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc gctttgacgc
    19441 ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct cgcgcattga
    19501 accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt gccacggttc
    19561 tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc aactgacggc
    19621 atctgcgggc gtggcaccag taaagtttct cgccaaaatc gcctccgaca tgaataaacc
    19681 caacggtcag tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact
    19741 ggaaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg
    19801 gacctgcggt gatgtacaaa agtgtgatct ggtgattctg cttaaacgct ttggcaaatt
    19861 tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg
    19921 gttacgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc actggtctga
    19981 atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa
    20041 acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt ttcagcagac
    20101 cacccaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa
    20161 aacctgggat gaacgccgcg gcgggcgcgg tgtgcgtctg gtggggctgc atgtgacgtt
    20221 gcttgacccg caaatggaaa gacaactggt gctgggatta tgatgtatac tattatgtat
    20281 attctggtgt gcattattat gagggtatca ctgtatgcat cgaattctcg ctgaaaaatc
    20341 ggtcaatatc actgagttac gtaaaaaccc agctaaatac tttattgatc aaccagttgc
    20401 ggttctttct aataatcgcc ccgcaggata tctcttaagt gccagcgcat tcgaagcgtt
    20461 aatggacatg cttgctgaac aagaggagaa aaagcccata aaggcgcgct ttcgtccaag
    20521 tgctgcaaga ttagaggaaa ttacacgccg cgctaaacaa tatcttaatg atatgacgga
    20581 tgatgatttc aatgacttta aggaataagg atgcgggtat tcaaaacaaa acttattcgc
    20641 ctgcaactta cagcagagga acttgatgcg ttaacggcgg attttatttc ctataagcgt
    20701 gacggtgttt tgccagatat atttggtcgc gatgcactct acgacgactc gtttacctgg
    20761 ccattaatca aatttgagcg agttgctcat attcatctgg caaatgtgaa taatccattt
    20821 ccgccacagt tgcgccaatt cagcagaacg aatgacgaag cgcatttggt atattgtcag
    20881 ggtgcgtttg atgagcaagc atggttgctc attgccattc tgaagcctga acctcataaa
    20941 ctggctcgag ataacaacca aatgcataaa attggaaaaa tggcagaagc gtttcgcatg
    21001 cgtttttgaa tttatattat gaataacata caaataagag attatcagcc tggcgatttt
    21061 cagcaactat gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca
    21121 ctacaacaaa ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg
    21181 aaatcacaag tgcgggttgc agttattaat gcaaaactgg tcggctttat tacctgcgtt
    21241 gaacactata tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc
    21301 gctttgttaa aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc
    21361 gcaaaaccct tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc
    21421 cggggagcgt ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt
    21481 gcaatgaaaa taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga
    21541 ggtaaatcat gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct
    21601 ttttttcttg ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa
    21661 ttcaagtcgc agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg
    21721 gcaattatat tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga
    21781 tcgagagttt agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg
    21841 tagtggggat gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct
    21901 ccgcaggccg tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat
    21961 ggcaaacaga tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag
    22021 ctttggatga cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac
    22081 agcatccgtg gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc
    22141 aacaagttga tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc
    22201 atctgcaact gctggttcat agcggctcga gggggctagg gcagtctatt ttacagcggc
    22261 atattgcctc gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg
    22321 cgcgttttaa tcgccacctg atcgctttga gcataatgca acaggttaag gccaccggaa
    22381 gtccggttct ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag
    22441 gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt
    22501 cacgcggtga ttactccagg ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc
    22561 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
    22621 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
    22681 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
    22741 gtcagggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
    22801 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
    22861 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
    22921 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
    22981 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
    23041 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
    23101 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
    23161 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
    23221 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
    23281 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
    23341 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
    23401 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
    23461 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
    23521 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
    23581 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
    23641 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
    23701 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
    23761 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
    23821 ctcaccacgt agtctttacc gctgtcgatt agtgaacgga tcaggcagtg aatttctacg
    23881 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
    23941 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
    24001 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
    24061 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
    24121 ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg
    24181 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
    24241 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
    24301 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
    24361 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
    24421 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
    24481 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc
    24541 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
    24601 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
    24661 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
    24721 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggttgacga
    24781 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
    24841 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
    24901 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
    24961 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
    25021 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
    25081 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    25141 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    25201 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    25261 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    25321 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    25381 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    25441 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    25501 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    25561 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
    25621 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    25681 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
    25741 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    25801 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    25861 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    25921 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
    25981 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
    26041 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
    26101 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
    26161 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
    26221 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
    26281 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg
    26341 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
    26401 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
    26461 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
    26521 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
    26581 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
    26641 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
    26701 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
    26761 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
    26821 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
    26881 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
    26941 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
    27001 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
    27061 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
    27121 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
    27181 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
    27241 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
    27301 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
    27361 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
    27421 ccggcgaatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
    27481 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
    27541 tcattattaa tattcagctt attatcgcta tccagttggt tgattttata atcaacaaac
    27601 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
    27661 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
    27721 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
    27781 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
    27841 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
    27901 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
    27961 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
    28021 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
    28081 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
    28141 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
    28201 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
    28261 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
    28321 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
    28381 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
    28441 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
    28501 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
    28561 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
    28621 aaagttttta acctgagatt gttaaagata tattacagat taatgatatt
//
LOCUS       DBBBSB010000048        28419 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.048, whole genome shotgun sequence.
ACCESSION   DBBBSB010000048 DBBBSB010000000
VERSION     DBBBSB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.048"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            563..1333
                     /locus_tag="ACIUS0_002017"
     CDS             563..1333
                     /locus_tag="ACIUS0_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGB0219769.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="ACIUS0_002018"
     CDS             1324..1797
                     /locus_tag="ACIUS0_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGB0219770.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="ACIUS0_002019"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="ACIUS0_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGB0219771.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="ACIUS0_002020"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="ACIUS0_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGB0219772.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /gene="yjjA"
                     /locus_tag="ACIUS0_002021"
     CDS             3232..3726
                     /gene="yjjA"
                     /locus_tag="ACIUS0_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGB0219773.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="ACIUS0_002022"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="ACIUS0_002022"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGB0219774.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACIUS0_002023"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="ACIUS0_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="ACIUS0_002024"
     CDS             7556..8470
                     /locus_tag="ACIUS0_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGB0219775.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="ACIUS0_002025"
     CDS             8685..10046
                     /locus_tag="ACIUS0_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219776.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACIUS0_002026"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="ACIUS0_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGB0219777.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACIUS0_002027"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="ACIUS0_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGB0219778.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /gene="hpaG"
                     /locus_tag="ACIUS0_002028"
     CDS             12596..13885
                     /gene="hpaG"
                     /locus_tag="ACIUS0_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGB0219779.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="ACIUS0_002029"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="ACIUS0_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGB0219780.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="ACIUS0_002030"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="ACIUS0_002030"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGB0219781.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="ACIUS0_002031"
     CDS             16211..16591
                     /locus_tag="ACIUS0_002031"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGB0219782.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="ACIUS0_002032"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="ACIUS0_002032"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGB0219783.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="ACIUS0_002033"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="ACIUS0_002033"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGB0219784.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="ACIUS0_002034"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="ACIUS0_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGB0219785.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="ACIUS0_002035"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="ACIUS0_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGB0219786.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="ACIUS0_002036"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="ACIUS0_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="ACIUS0_002037"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="ACIUS0_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="ACIUS0_002038"
     CDS             23320..23832
                     /locus_tag="ACIUS0_002038"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGB0219787.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="ACIUS0_002039"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="ACIUS0_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGB0219788.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="ACIUS0_002040"
     CDS             26425..26628
                     /locus_tag="ACIUS0_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGB0219789.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="ACIUS0_002041"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="ACIUS0_002041"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGB0219790.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28419)
                     /locus_tag="ACIUS0_002042"
     CDS             complement(27664..>28419)
                     /locus_tag="ACIUS0_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HGB0219791.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYRLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6953 a         7426 c         7127 g         6913 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccagcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccag gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcccgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagcctgt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtata
//
LOCUS       DBBBSB010000049        28071 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.049, whole genome shotgun sequence.
ACCESSION   DBBBSB010000049 DBBBSB010000000
VERSION     DBBBSB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.049"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..209)
                     /locus_tag="ACIUS0_002043"
     CDS             complement(<1..209)
                     /locus_tag="ACIUS0_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGB0219792.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACIUS0_002044"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACIUS0_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGB0219793.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACIUS0_002045"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACIUS0_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGB0219794.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACIUS0_002046"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACIUS0_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGB0219795.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACIUS0_002047"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACIUS0_002047"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGB0219796.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACIUS0_002048"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACIUS0_002048"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGB0219797.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACIUS0_002049"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACIUS0_002049"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGB0219798.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACIUS0_002050"
     CDS             complement(4598..5320)
                     /locus_tag="ACIUS0_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0219799.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACIUS0_002051"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACIUS0_002051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGB0219800.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACIUS0_002052"
     CDS             6520..6738
                     /locus_tag="ACIUS0_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGB0219801.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACIUS0_002053"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACIUS0_002053"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGB0219802.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACIUS0_002054"
     CDS             complement(7472..7672)
                     /locus_tag="ACIUS0_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGB0219803.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACIUS0_002055"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACIUS0_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACIUS0_002056"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACIUS0_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGB0219804.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACIUS0_002057"
     CDS             10101..12014
                     /locus_tag="ACIUS0_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0219805.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACIUS0_002058"
     CDS             12014..13036
                     /locus_tag="ACIUS0_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGB0219806.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACIUS0_002059"
     CDS             13030..13248
                     /locus_tag="ACIUS0_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGB0219807.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACIUS0_002060"
     CDS             13302..14171
                     /locus_tag="ACIUS0_002060"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGB0219808.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACIUS0_002061"
     CDS             complement(14226..14630)
                     /locus_tag="ACIUS0_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGB0219809.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACIUS0_002062"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACIUS0_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGB0219810.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACIUS0_002063"
     CDS             15615..17705
                     /locus_tag="ACIUS0_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGB0219811.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACIUS0_002064"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACIUS0_002064"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGB0219812.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACIUS0_002065"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACIUS0_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGB0219813.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACIUS0_002066"
     CDS             complement(19673..20275)
                     /locus_tag="ACIUS0_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGB0219814.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACIUS0_002067"
     CDS             complement(20265..20432)
                     /locus_tag="ACIUS0_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGB0219815.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACIUS0_002068"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACIUS0_002068"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGB0219816.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="ACIUS0_002069"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="ACIUS0_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGB0219817.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="ACIUS0_002070"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="ACIUS0_002070"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGB0219818.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="ACIUS0_002071"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="ACIUS0_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGB0219819.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="ACIUS0_002072"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="ACIUS0_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGB0219820.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cysG"
                     /locus_tag="ACIUS0_002073"
     CDS             26649..28022
                     /gene="cysG"
                     /locus_tag="ACIUS0_002073"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGB0219821.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6803 a         7509 c         7087 g         6672 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatagggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata t
//
LOCUS       DBBBSB010000050        27601 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.050, whole genome shotgun sequence.
ACCESSION   DBBBSB010000050 DBBBSB010000000
VERSION     DBBBSB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.050"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACIUS0_002074"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACIUS0_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGB0219822.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACIUS0_002075"
     CDS             complement(1614..1937)
                     /locus_tag="ACIUS0_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGB0219823.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACIUS0_002076"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACIUS0_002076"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGB0219824.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRAVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACIUS0_002077"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACIUS0_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGB0219825.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACIUS0_002078"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACIUS0_002078"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGB0219826.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACIUS0_002079"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACIUS0_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGB0219827.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACIUS0_002080"
     CDS             7537..8574
                     /locus_tag="ACIUS0_002080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGB0219828.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACIUS0_002081"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACIUS0_002081"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGB0219829.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACIUS0_002082"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACIUS0_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGB0219830.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACIUS0_002083"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACIUS0_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGB0219831.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACIUS0_002084"
     CDS             10745..11626
                     /locus_tag="ACIUS0_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0219832.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACIUS0_002085"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACIUS0_002085"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGB0219833.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACIUS0_002086"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACIUS0_002086"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGB0219834.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACIUS0_002087"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACIUS0_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACIUS0_002088"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACIUS0_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGB0219835.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACIUS0_002089"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACIUS0_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGB0219836.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACIUS0_002090"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACIUS0_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGB0219837.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHVDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACIUS0_002091"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACIUS0_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGB0219838.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACIUS0_002092"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACIUS0_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGB0219839.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACIUS0_002093"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACIUS0_002093"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGB0219840.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACIUS0_002094"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACIUS0_002094"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGB0219841.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACIUS0_002095"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACIUS0_002095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGB0219842.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACIUS0_002096"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACIUS0_002096"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGB0219843.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACIUS0_002097"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACIUS0_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGB0219844.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACIUS0_002098"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACIUS0_002098"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGB0219845.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACIUS0_002099"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACIUS0_002099"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGB0219846.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACIUS0_002100"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACIUS0_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGB0219847.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACIUS0_002101"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACIUS0_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGB0219848.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACIUS0_002102"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACIUS0_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6807 a         6864 c         7239 g         6691 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac ggcgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atgtcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DBBBSB010000051        27383 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.051, whole genome shotgun sequence.
ACCESSION   DBBBSB010000051 DBBBSB010000000
VERSION     DBBBSB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27383
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.051"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..576
                     /locus_tag="ACIUS0_002103"
     CDS             <1..576
                     /locus_tag="ACIUS0_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0219849.1"
                     /translation="LAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLC
                     MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLD
                     NLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEK
                     GSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="ACIUS0_002104"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="ACIUS0_002104"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGB0219850.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACIUS0_002105"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACIUS0_002105"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGB0219851.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACIUS0_002106"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACIUS0_002106"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGB0219852.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACIUS0_002107"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACIUS0_002107"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0219853.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACIUS0_002108"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACIUS0_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGB0219854.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="ACIUS0_002109"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="ACIUS0_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGB0219855.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="ACIUS0_002110"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="ACIUS0_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGB0219856.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="ACIUS0_002111"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="ACIUS0_002111"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGB0219857.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="ACIUS0_002112"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="ACIUS0_002112"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGB0219858.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="ACIUS0_002113"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="ACIUS0_002113"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGB0219859.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="ACIUS0_002114"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="ACIUS0_002114"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGB0219860.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="ACIUS0_002115"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="ACIUS0_002115"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGB0219861.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACIUS0_002116"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="ACIUS0_002116"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGB0219862.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="ACIUS0_002117"
     CDS             13936..14316
                     /locus_tag="ACIUS0_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGB0219863.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="ACIUS0_002118"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="ACIUS0_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGB0219864.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACIUS0_002119"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="ACIUS0_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGB0219865.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACIUS0_002120"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="ACIUS0_002120"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGB0219866.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="ACIUS0_002121"
     tRNA            complement(17417..17492)
                     /locus_tag="ACIUS0_002121"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="ACIUS0_002122"
     tRNA            complement(17496..17571)
                     /locus_tag="ACIUS0_002122"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="ACIUS0_002123"
     tRNA            complement(17623..17698)
                     /locus_tag="ACIUS0_002123"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="ACIUS0_002124"
     tRNA            complement(17701..17776)
                     /locus_tag="ACIUS0_002124"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="ACIUS0_002125"
     tRNA            complement(17912..17987)
                     /locus_tag="ACIUS0_002125"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACIUS0_002126"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="ACIUS0_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGB0219867.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACIUS0_002127"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="ACIUS0_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGB0219868.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACIUS0_002128"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="ACIUS0_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGB0219869.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACIUS0_002129"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="ACIUS0_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGB0219870.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACIUS0_002130"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="ACIUS0_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGB0219871.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACIUS0_002131"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="ACIUS0_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGB0219872.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACIUS0_002132"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="ACIUS0_002132"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGB0219873.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACIUS0_002133"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="ACIUS0_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGB0219874.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACIUS0_002134"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="ACIUS0_002134"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGB0219875.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACIUS0_002135"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="ACIUS0_002135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGB0219876.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACIUS0_002136"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="ACIUS0_002136"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGB0219877.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         6891 a         7261 c         6705 g         6526 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caa
//
LOCUS       DBBBSB010000052        27060 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.052, whole genome shotgun sequence.
ACCESSION   DBBBSB010000052 DBBBSB010000000
VERSION     DBBBSB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27060
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.052"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..175)
                     /locus_tag="ACIUS0_002137"
     CDS             complement(<1..175)
                     /locus_tag="ACIUS0_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGB0219878.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACIUS0_002138"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACIUS0_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGB0219879.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACIUS0_002139"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACIUS0_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGB0219880.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACIUS0_002140"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACIUS0_002140"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGB0219881.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACIUS0_002141"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACIUS0_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGB0219882.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACIUS0_002142"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACIUS0_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGB0219883.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="ACIUS0_002143"
     CDS             complement(5179..5421)
                     /locus_tag="ACIUS0_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGB0219884.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACIUS0_002144"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACIUS0_002144"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGB0219885.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACIUS0_002145"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACIUS0_002145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGB0219886.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="ACIUS0_002146"
     CDS             complement(8338..9312)
                     /locus_tag="ACIUS0_002146"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGB0219887.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="ACIUS0_002147"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="ACIUS0_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGB0219888.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="ACIUS0_002148"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="ACIUS0_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGB0219889.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="ACIUS0_002149"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="ACIUS0_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGB0219890.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="ACIUS0_002150"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="ACIUS0_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGB0219891.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="ACIUS0_002151"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="ACIUS0_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGB0219892.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="ACIUS0_002152"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="ACIUS0_002152"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGB0219893.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="ACIUS0_002153"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="ACIUS0_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGB0219894.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="ACIUS0_002154"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="ACIUS0_002154"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGB0219895.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACIUS0_002155"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACIUS0_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGB0219896.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /gene="aaeX"
                     /locus_tag="ACIUS0_002156"
     CDS             23179..23382
                     /gene="aaeX"
                     /locus_tag="ACIUS0_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGB0219897.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="ACIUS0_002157"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="ACIUS0_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGB0219898.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="ACIUS0_002158"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="ACIUS0_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGB0219899.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="ACIUS0_002159"
     CDS             26387..26659
                     /locus_tag="ACIUS0_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGB0219900.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="ACIUS0_002160"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="ACIUS0_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGB0219901.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6449 a         6789 c         7230 g         6592 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaactggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatctgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagagtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgggcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctcac cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac tggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg acaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
//
LOCUS       DBBBSB010000053        26409 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.053, whole genome shotgun sequence.
ACCESSION   DBBBSB010000053 DBBBSB010000000
VERSION     DBBBSB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26409
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.053"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACIUS0_002161"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACIUS0_002161"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGB0219902.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACIUS0_002162"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACIUS0_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGB0219903.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACIUS0_002163"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACIUS0_002163"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGB0219904.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACIUS0_002164"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACIUS0_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGB0219905.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACIUS0_002165"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACIUS0_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGB0219906.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACIUS0_002166"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACIUS0_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGB0219907.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACIUS0_002167"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACIUS0_002167"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGB0219908.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="ACIUS0_002168"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="ACIUS0_002168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGB0219909.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="ACIUS0_002169"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="ACIUS0_002169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGB0219910.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="ACIUS0_002170"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="ACIUS0_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGB0219911.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="ACIUS0_002171"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="ACIUS0_002171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGB0219912.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="ACIUS0_002172"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="ACIUS0_002172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGB0219913.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="ACIUS0_002173"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="ACIUS0_002173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGB0219914.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="ACIUS0_002174"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="ACIUS0_002174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGB0219915.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="ACIUS0_002175"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="ACIUS0_002175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGB0219916.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /locus_tag="ACIUS0_002176"
     CDS             15215..15634
                     /locus_tag="ACIUS0_002176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGB0219917.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="ACIUS0_002177"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="ACIUS0_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGB0219918.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="ACIUS0_002178"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="ACIUS0_002178"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGB0219919.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="ACIUS0_002179"
     CDS             19508..20329
                     /locus_tag="ACIUS0_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HGB0219920.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="ACIUS0_002180"
     CDS             20316..22424
                     /locus_tag="ACIUS0_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HGB0219921.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="ACIUS0_002181"
     CDS             22421..24088
                     /locus_tag="ACIUS0_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGB0219922.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="ACIUS0_002182"
     CDS             24091..25617
                     /locus_tag="ACIUS0_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HGB0219923.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..>26409
                     /locus_tag="ACIUS0_002183"
     CDS             25618..>26409
                     /locus_tag="ACIUS0_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HGB0219924.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTA"
BASE COUNT         6801 a         6201 c         6814 g         6593 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agcacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagct
//
LOCUS       DBBBSB010000054        25895 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.054, whole genome shotgun sequence.
ACCESSION   DBBBSB010000054 DBBBSB010000000
VERSION     DBBBSB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25895
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.054"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACIUS0_002184"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACIUS0_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGB0219925.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACIUS0_002185"
     CDS             348..641
                     /locus_tag="ACIUS0_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGB0219926.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACIUS0_002186"
     CDS             713..1372
                     /locus_tag="ACIUS0_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGB0219927.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACIUS0_002187"
     CDS             1464..1910
                     /locus_tag="ACIUS0_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGB0219928.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACIUS0_002188"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACIUS0_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="ACIUS0_002189"
     CDS             3390..3809
                     /locus_tag="ACIUS0_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGB0219929.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="ACIUS0_002190"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="ACIUS0_002190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGB0219930.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACIUS0_002191"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACIUS0_002191"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGB0219931.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACIUS0_002192"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACIUS0_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGB0219932.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="ACIUS0_002193"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="ACIUS0_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGB0219933.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="ACIUS0_002194"
     CDS             complement(8332..9864)
                     /locus_tag="ACIUS0_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGB0219934.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="ACIUS0_002195"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="ACIUS0_002195"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGB0219935.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="ACIUS0_002196"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="ACIUS0_002196"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGB0219936.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="ACIUS0_002197"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="ACIUS0_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="ACIUS0_002198"
     CDS             complement(14765..16501)
                     /locus_tag="ACIUS0_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGB0219937.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACIUS0_002199"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACIUS0_002199"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGB0219938.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="ACIUS0_002200"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="ACIUS0_002200"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGB0219939.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="ACIUS0_002201"
     CDS             complement(18223..18957)
                     /locus_tag="ACIUS0_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGB0219940.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="ACIUS0_002202"
     CDS             19158..19412
                     /locus_tag="ACIUS0_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGB0219941.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="ACIUS0_002203"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="ACIUS0_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACIUS0_002204"
     CDS             complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACIUS0_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HGB0219942.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="ACIUS0_002205"
     CDS             complement(22296..23105)
                     /locus_tag="ACIUS0_002205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGB0219943.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="ACIUS0_002206"
     CDS             complement(23115..23873)
                     /locus_tag="ACIUS0_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0219944.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="ACIUS0_002207"
     CDS             complement(23870..24850)
                     /locus_tag="ACIUS0_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGB0219945.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="ACIUS0_002208"
     CDS             complement(24850..25872)
                     /locus_tag="ACIUS0_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HGB0219946.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6456 a         6370 c         6726 g         6343 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaa
//
LOCUS       DBBBSB010000055        25703 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.055, whole genome shotgun sequence.
ACCESSION   DBBBSB010000055 DBBBSB010000000
VERSION     DBBBSB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25703
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.055"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACIUS0_002209"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACIUS0_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACIUS0_002210"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACIUS0_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGB0219947.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGDDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACIUS0_002211"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACIUS0_002211"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGB0219948.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACIUS0_002212"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACIUS0_002212"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGB0219949.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACIUS0_002213"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACIUS0_002213"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGB0219950.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACIUS0_002214"
     tRNA            7783..7858
                     /locus_tag="ACIUS0_002214"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="ACIUS0_002215"
     tRNA            8016..8091
                     /locus_tag="ACIUS0_002215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="ACIUS0_002216"
     tRNA            8127..8202
                     /locus_tag="ACIUS0_002216"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8238..8313
                     /locus_tag="ACIUS0_002217"
     tRNA            8238..8313
                     /locus_tag="ACIUS0_002217"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8271..8273,aa:Gly,seq:gcc)
     gene            8547..8600
                     /locus_tag="ACIUS0_002218"
     CDS             8547..8600
                     /locus_tag="ACIUS0_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0219951.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8583..9722)
                     /gene="queG"
                     /locus_tag="ACIUS0_002219"
     CDS             complement(8583..9722)
                     /gene="queG"
                     /locus_tag="ACIUS0_002219"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGB0219952.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9721..11268
                     /gene="nnr"
                     /locus_tag="ACIUS0_002220"
     CDS             9721..11268
                     /gene="nnr"
                     /locus_tag="ACIUS0_002220"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGB0219953.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11240..11701
                     /gene="tsaE"
                     /locus_tag="ACIUS0_002221"
     CDS             11240..11701
                     /gene="tsaE"
                     /locus_tag="ACIUS0_002221"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGB0219954.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11720..13057
                     /gene="amiB"
                     /locus_tag="ACIUS0_002222"
     CDS             11720..13057
                     /gene="amiB"
                     /locus_tag="ACIUS0_002222"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGB0219955.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            13067..14914
                     /gene="mutL"
                     /locus_tag="ACIUS0_002223"
     CDS             13067..14914
                     /gene="mutL"
                     /locus_tag="ACIUS0_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGB0219956.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14907..15857
                     /gene="miaA"
                     /locus_tag="ACIUS0_002224"
     CDS             14907..15857
                     /gene="miaA"
                     /locus_tag="ACIUS0_002224"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGB0219957.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15943..16251
                     /gene="hfq"
                     /locus_tag="ACIUS0_002225"
     CDS             15943..16251
                     /gene="hfq"
                     /locus_tag="ACIUS0_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGB0219958.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16327..17607
                     /gene="hflX"
                     /locus_tag="ACIUS0_002226"
     CDS             16327..17607
                     /gene="hflX"
                     /locus_tag="ACIUS0_002226"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGB0219959.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17693..18952
                     /gene="hflK"
                     /locus_tag="ACIUS0_002227"
     CDS             17693..18952
                     /gene="hflK"
                     /locus_tag="ACIUS0_002227"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGB0219960.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18955..19959
                     /gene="hflC"
                     /locus_tag="ACIUS0_002228"
     CDS             18955..19959
                     /gene="hflC"
                     /locus_tag="ACIUS0_002228"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGB0219961.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            20041..20238
                     /locus_tag="ACIUS0_002229"
     CDS             20041..20238
                     /locus_tag="ACIUS0_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGB0219962.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20342..21640
                     /gene="purA"
                     /locus_tag="ACIUS0_002230"
     CDS             20342..21640
                     /gene="purA"
                     /locus_tag="ACIUS0_002230"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGB0219963.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21845..22270
                     /gene="nsrR"
                     /locus_tag="ACIUS0_002231"
     CDS             21845..22270
                     /gene="nsrR"
                     /locus_tag="ACIUS0_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGB0219964.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22309..24750
                     /gene="rnr"
                     /locus_tag="ACIUS0_002232"
     CDS             22309..24750
                     /gene="rnr"
                     /locus_tag="ACIUS0_002232"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGB0219965.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24930..25661
                     /gene="rlmB"
                     /locus_tag="ACIUS0_002233"
     CDS             24930..25661
                     /gene="rlmB"
                     /locus_tag="ACIUS0_002233"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGB0219966.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         6019 a         6513 c         7217 g         5954 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgtcaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aagtgctgta
     8221 aggcacagac cacccaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg
     8281 gggtcgcgag ttcgagtctc gtttcccgct ccaaattctt ctctcaataa aatatctaca
     8341 gcgacgcgat gcgttattgc tggtttttgt tgtctctgac aaactcttgt aaacagagtt
     8401 atccacagcc tcaggctgta atcttaattt caaagaaact tcgcacggtg aatggtattt
     8461 ttttaaccta ttgataaata agttaaaaat caagattccg ttttgtcgag tcgatcactt
     8521 gacgatttta ttcgtcttga attgcaatgc gtttttattt ttattcacaa gctgtggatg
     8581 aatcaggcgt cacgcggtaa ccctttttca atcacccgaa ccagacgctg ttttttcggc
     8641 aattgcactt cgactatgca cgcatttcgt ctctctattt gctgcgcaat cgcccacgct
     8701 atgtgctcat cgagaagtgg gtgctcacct ttacgacttt ccagcgctgt caaaatcgtt
     8761 tcatcccaag gggcattgcc taatgccacg gcgatattac gcagccagcg caggtgacct
     8821 atacggcgaa tcgccgatcc ttccgtgact tttaaaaact tctcttcgct ccaggcaaat
     8881 aactcaatga gttccggtgc gtgtagcggc ttacgcgggc tgaaatcgtc ttctgtagtg
     8941 agttgcgaat agcgattcca cgggcagata agctggcagt catcgcaacc gtaaatacgg
     9001 tttcccatta acggtcgcaa ctcttccggg agcgcccctt caagttcgat ggtgagataa
     9061 gagatacagc ggcgagcatc gacggtatat ggctcgacga tggcaccggt cgggcaaatc
     9121 gtcatacagg ccacgcattt gccgcatcct tcctcgactg gttgatccac gggcagcgga
     9181 atatcgacca gcaattcgcc taaaaagaag aacgaaccgg cctcgcgatt gaggataagt
     9241 gagtgcttac ctgtccagcc gagcccagct ttttcagcta acgggcgctc gagaataggc
     9301 gcagaatcga caaacggtct aaaattcagc gaaacacaat gttgctgaat catctcgccc
     9361 agctttttga gtcggttgcg cagaagtttg tgatagtcac ggcccagcgc ataacggcta
     9421 acatagccga gtttggggtt tttcagcgtg ctggcaaatg cggcgttagc aggaaggtaa
     9481 ttcatccgca cgctgattac acgcagcgta ccgggtaata actcatgggg gcgagcgcgc
     9541 agcataccgt gacgtgccat ccagtccatt tcgccgtggt attgtttgtc cagccatgct
     9601 tgcagtttgg gctcggactc gctgagatcg gtatcggtaa tacctacctg ctgaaagccc
     9661 agttccagcc cccactgttt aattttttgc gctaactgat tgagatcgag gggctctgac
     9721 atgacggacc atacaatgaa gaaaaacccc gtaagtatac cacacaccgt ctggtacgcc
     9781 gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc tggggctcac actctatgag
     9841 ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc gttcggcgta tcctgacgcc
     9901 cgccactggc tggtgctgtg cggtcatggt aataacggcg gcgatggcta cgtggtcgcg
     9961 cgactggcca aagcggtcgg cattgaggtc acgttgttgg cccaggagag cgacaaaccg
    10021 ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa acgcgggtgg cgagatccat
    10081 gcttcgaata ttgtctggcc cgaatcggta gatctgattg ttgatgcgct gctcggtacc
    10141 ggcttgcagc aagcgccccg cgaatccatt agccagttaa tcgaccacgc taattcccat
    10201 cctgcgccga ttgtggcggt tgatatccct tccggcctgc tggctgaaac cggcgctacg
    10261 ccaggcgcag tgatcaacgc cgatcacacc atcactttta ttgcgctgaa actaggcttg
    10321 ctcactggaa aagcgcggga tgttacagga caactgcatt ttgactcact ggggctggat
    10381 agctggctgg caggtcagga gacgaaaatt cagcggtttt cggcagaaca actttctcac
    10441 tggctgaaac cgcgtcgccc gacttcgcat aaaggcgatc acgggcggct ggtgattatc
    10501 ggtggcgatc acggcacggc gggggctatt cgtatgacgg gggaagcggc gctacgtgct
    10561 ggtgctggtt tagtccgagt actgacccgc agtgagaaca ttgcgccgct gctgactgca
    10621 cgaccagaat tgatggtgca tgaactgact atggactctc ttaccgaaag cctggaatgg
    10681 gccgatgtgg tggtgattgg tcccggtctg ggccagcaag agtgggggaa aaaagccctg
    10741 caaaaagttg agaattttcg caaaccgatg ttgtgggatg ccgatgcatt gaacctgctg
    10801 gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc cgcatcctag cgaggccgca
    10861 cggttgttag gctgttccgt cgctgaaatt gaaagtgacc gcttacattg cgccaaacgt
    10921 ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg ccggaaccgt ggtcgccgcc
    10981 catcctgacg ctttaggcat tattgatgcc ggaaatgcag gcatggcgag cggcggcatg
    11041 ggcgatgtgc tctctggtat tattggcgca ttgcttgggc aaaaactgtc gccgtatgat
    11101 gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg acgtactggc ggcgcgtttt
    11161 ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc tacagcgtat tgttaacccg
    11221 gaagtgactg ataaaaacca tgatgaatcg agtaattccg ctccctgatg agcaggcaac
    11281 attagacctg ggcgagcggg tagcgaaagc ctgcgatggc gcaaccgtaa tctatctgta
    11341 tggcgattta ggcgcaggta aaaccacctt tagccggggc tttttacagg ctctgggtca
    11401 tcagggtaat gtcaaaagcc ccacttatac gctggtcgaa ccctatacgc tcgacaactt
    11461 aatggtctat cactttgatt tgtaccgcct tgccgatccc gaggagctgg agtttatggg
    11521 gatccgcgat tattttgcca acgatgccat ctgcctggtg gagtggccac aacaaggtac
    11581 aggtgttctt cctgacccgg atgtcgaaat acacattgat tatcaggcac aaggccgtga
    11641 ggcgcgcgtg agtgcggttt cctctgcggg tgaattgttg ctggcgcgtt tagccggtta
    11701 acctttgaaa ggtggcggga tgatgtatcg catcagaaat tggttggtag cgacgctgct
    11761 gctgctgtgc acgccggtgg gtgccgcgac gctctctgat attcaggttt ctaacggtaa
    11821 tcaacaggcg cggataacgt tgagttttat tggcgatcct gattatgcgt ttagccatca
    11881 aagcaaacgc accgtggcgc tcgatatcaa acaaacgggc atgattcagg gactgccgtt
    11941 gttgttcagc ggcaataatc tggtgaaggc gattcgctct ggaacgccta aagatgcaca
    12001 aacgctacgg ctggtggtcg atcttaccga aaacggtaaa accgaagcgg tgaagcggca
    12061 gaatggcagc aattacactg tcgtctttac gattaacgcc gatgtgccgc caccgcctcc
    12121 tccgccgccc gtggttgcga aacgcgttga aacgcctgcg gttgtcgcac cgcgcgtcag
    12181 cgaaccggcg cgcaatccgt ttaaaacgga aagtaaccgc actacgggtg ttatcagcag
    12241 taatacggta acgcgtccgg cagcgcgcgc gacggctaac actggcgata aaattatcat
    12301 cgctattgat gccggacacg gcggtcagga ccctggcgct atcggccccg gtggtacgcg
    12361 ggagaaaaat gtcaccatcg ccatcgcgcg taaattgcgt actttgctca atgacgatcc
    12421 gatgtttaaa ggcgttttaa cccgtgacgg ggattacttt atctcggtga tggggcgcag
    12481 tgatgtggca cataagcaaa acgccaattt cctcgtgtcg attcacgctg atgccgcacc
    12541 gaaccgtagt gcgactggcg cttccgtatg ggtgctctct aaccgtcgcg ccaacagtga
    12601 aatggccagc tggctggagc agcacgagaa actgtcggag ctgctgggtg gggcgggtga
    12661 tgtgctggcg aacagtcagt ctgaccccta tttaagccag gcggtgctgg atttacagtt
    12721 cggtcattcc cagcgggtag ggtatgatgt agcgaccagt atgatcagtc agttgcaacg
    12781 cattggcgaa atacataaac gtcgaccaga acacgccagc cttggcgttc tgcgctcgcc
    12841 ggatatccca tcagtactgg tcgaaaccgg ttttatcagc aacaacagcg aagaacgttt
    12901 gctggcgagc gacgattacc aacaacagct ggcagaagcc atttacaaag gcctgcgcaa
    12961 ttacttcctt gcgcatccga tgcaatctgc gccgcagggt gcaacggcac aaactgccag
    13021 tacggtgacg acgccagatc gcacgctgcc aaactaagga cgattgatgc caattcaggt
    13081 cttaccgcca caactggcga accagattgc cgcaggtgag gtggtcgagc gacctgcgtc
    13141 ggtagtcaaa gaactagtgg aaaacagcct cgatgcaggt gcgacgcgta tcgatattga
    13201 tatcgaacgc ggtggggcga aacttatccg cattcgtgat aacggctgcg gtatcaaaaa
    13261 agatgagctg gcgctggcgc tggctcgtca tgccaccagt aaaatcgcct ctctggacga
    13321 tctcgaagcc attatcagcc tgggctttcg cggtgaggcg ctggcgagta tcagttcggt
    13381 ttcccgcctg acgctcactt cacgcaccgc agaacagcag gaagcctggc aggcctatgc
    13441 cgaaggacgc gacatggacg tgacggttaa accggcagcg catccggtgg gaacgacgct
    13501 ggaggtgctg gatctgttct acaacacccc ggcgcggcgc aaattcctgc gcaccgagaa
    13561 aaccgaattt agccatattg atgaaatcat ccgccgcatt gcgctggcgc gtttcgacgt
    13621 cacgatcaac ctgtcgcata acggtaaaat tgtgcgtcag taccgcgcag tgccggaagg
    13681 cgggcaaaaa gaacggcgct taggcgcgat ttgcggcacc gcttttctcg aacaagcgct
    13741 ggcgattgaa tggcaacacg gcgatctcac cctgcgcggc tgggtggccg atccaaatca
    13801 cactacgccc gcactggcgg aaattcagta ttgctacgtg aatggtcgca tgatgcgcga
    13861 tcgcctgatc aatcatgcga tccgccaggc ctgcgaagac aaactggggg ccgatcagca
    13921 accggcattt gtgttgtatc tggagatcga cccgcatcag gtggacgtca acgtacaccc
    13981 cgccaaacac gaagtgcgtt tccatcagtc gcgtctggtg catgatttta tctatcaggg
    14041 cgtgctgagc gtgctacaac agcaactgga aacgccgcta ccgctggacg atgaaccaca
    14101 acccgcaccg cgtgccatcc cggaaaaccg cgtggcggcg gggcgcaatc actttgcaga
    14161 accggcagct cgtgagccgg tagctccgcg ctactctcct gcgcccgcct caggcagtcg
    14221 tccggctgcc ccctggccga atgcgcagcc aggctaccag aaacagcaag gtgaagtgta
    14281 tcgccagctt ttgcaaacgc ccgcgccgat gcaaaaacca aaagcgccgg aaccgcagga
    14341 acctgcactt gcggcgaaca gtcagagttt tggtcgggta ctgactatcg tccattccga
    14401 ctgtgcgttg ctggagcgcg atggcaacat ttcactttta tccttgccag tggcagaacg
    14461 ttggctgcgt caggcacaat tgacgccggg tgaagcgccc gtttgcgccc agccgctgct
    14521 gattccgttg cggctaaaag tttctggcga agaaaaatcg gcattagaaa aagcgcagtc
    14581 tgccctagcg gaattgggta ttgatttcca gtcagatgca cagcatgtga ccatcagggc
    14641 cgtgccttta cccttacgcc aacaaaattt acaaatcttg attcctgaac tgataggcta
    14701 cctggcgaag cagtccgtat tcgaacctgg caatattgcg cagtggattg cacgaaatct
    14761 gatgagcgaa catgcgcagt ggtcaatggc acaggccata accctgctgg cggacgtgga
    14821 acggttatgt ccgcaacttg tgaaaacgcc gccgggtggt ctgttacaat ctgttgattt
    14881 acatccggcg ataaaagccc tgaaagatga gtgatatcag taaggcgagc ctgcctaagg
    14941 cgattttttt gatggggccg acggcctccg gtaaaacggc gttagccatt gagctgcgta
    15001 aaattttacc agtagagttg ataagcgttg attctgtcct tatttacaaa gggatggata
    15061 tcgggacggc gaagccgaac gctgaagagt tactcgccgc gccgcaccga ttgctggata
    15121 ttcgcgatcc gtcgcaggct tactcggctg ctgattttcg ccgcgatgcg ctggcggaaa
    15181 tggccgatat caccgcggcg gggcggatcc cactgttagt gggcggtacg atgttgtatt
    15241 tcaaggcatt gctggaaggg ttgtcgccgc taccgtcggc agacccggaa gtgcgggcca
    15301 gaattgagca acaggcggca gagcaaggtt gggagtcatt gcatcgtcaa cttcaggagg
    15361 tagatccggt tgcggcagca aggattcatc caaatgatcc acaaaggctt tcccgggcac
    15421 tggaagtttt tttcatttcg ggtaaaactt taacggaact gacgcaaacg tcaggagacg
    15481 ctctaccgta tcaggtgcat cagttcgcca tcgccccggc gagccgtgaa ctgctccatc
    15541 aacgcattga gcagcgtttt catcagatgt tggcttcagg ttttgaagca gaagtccggg
    15601 cgctttttgc ccgaggagat ttgcatacgg acttgccttc cattcgttgc gtgggttatc
    15661 gccagatgtg gtcttacctt gaaggcgaaa tctcatacga tgaaatggtt tatcgaggtg
    15721 tttgcgccac gagacagttg gcgaagcggc agataacctg gctgcgtggt tgggaagggg
    15781 ttcactggct tgacagtgaa aaaccagaac aggcgcgtga cgaagtatta caggttgttg
    15841 gtgctatcgc aggctgaatg tgtacaattg agacgtatcg tgcgcaattt tttcagaatc
    15901 gaaaggttca aagtacaaat aagcatataa ggaaaagaga gaatggctaa ggggcaatct
    15961 ttacaagatc cgttcctgaa cgcactgcgt cgggaacgtg ttccagtttc tatttatttg
    16021 gtgaatggta ttaagctgca agggcaaatc gagtcttttg atcagttcgt gatcctgttg
    16081 aaaaacacgg tcagccagat ggtttacaag cacgcgattt ctactgttgt cccatctcgc
    16141 ccggtttctc atcacagtaa caacgccggt ggcggtacca gcagtaacta ccatcatggt
    16201 agcagcgcgc agaatacttc cgcgcaacag gacagcgaag aaaccgaata aggtttcggg
    16261 ctgttttttt acacggggag ccagcgatcc tgcgttcccc gctgatctat ttagagggtt
    16321 atacgcttgt ttgaccgtta tgatgctggt gagcaggcgg tactggtaca catctatttt
    16381 acgcaagaca aagatatgga agacctccag gagtttgaat ctctggtctc ttccgccggt
    16441 gtcgaagcat tgcaggtgat taccggtagc cgtaaagcgc cgcacccaaa gtattttgta
    16501 ggtgaaggta aagcagttga aattgcggaa gctgtcaaag cgatgggtgc ttcggtcgtt
    16561 ctttttgacc atgccctgag cccggcgcaa gagcgtaacc tggagcgttt gtgcgagtgt
    16621 cgtgttatcg accgcaccgg ccttatttta gatattttcg cccaacgtgc gcgtacccat
    16681 gagggtaagt tgcaggttga gctggcgcag ctgcgccatc tggctacgcg cctggtgcgt
    16741 ggctggaccc accttgaaag acagaaaggc gggataggtt tgcgtggtcc gggtgaaacc
    16801 cagctcgaaa ccgaccgtcg tttgttgcgt aatcgcatcg tgcagataca gtcgcgcctg
    16861 gaaagagttg aaaagcagcg tgagcagggg cggcaatcgc gtatcaaagc cgacgttcct
    16921 actgtttcgc tggtgggata taccaacgcc ggtaaatcta cccttttcaa tcgcatcacc
    16981 gaagcgcggg tctacgcggc agaccagttg tttgccaccc tcgacccgac gttgcggcgt
    17041 attgacgttg cagatgtcgg tgaaaccgta cttgcagata ccgtagggtt tattcgccac
    17101 ctgccgcacg atctggtggc ggcatttaaa gccacgttac aagagacgcg gcaagccaca
    17161 ttactgctgc acgtcattga tgcggcggat gtgcgtgtac aagaaaacat cgaagcggtg
    17221 aatacggttc ttgaagagat cgacgctcac gagatcccaa ccctgctggt gatgaacaag
    17281 atcgatatgc tggaagattt cgaaccgcgt attgatcggg acgaagagaa caaaccgatc
    17341 cgtgtctggc tttccgcaca gaccggagcg gggataccac agctttttca ggctttgacg
    17401 gagcggcttt ccggcgaggt ggcgcagcat acattgcgtc tgccaccgca ggaagggcgt
    17461 ctgaggagtc gtttttatca gcttcaggca atagaaaaag agtggatgga ggaggacggc
    17521 agcgtaagtc tgcaagttcg tatgccgatc gttgactggc gtcgcctctg taaacaagaa
    17581 ccggcgttga tcgattacct gatctaacgg cgtagcgtct gaagcgtgga gtcatatcct
    17641 ctggcgtcga aagacaacag ggatcaccgc ataacaaata tggagcacaa acatggcgtg
    17701 gaatcagccc ggtaataacg gacaagaccg cgacccgtgg ggaagcagca aacctggcgg
    17761 caactctgag ggaaatggaa acaaaggcgg tcgcgatcaa gggccacctg atttagatga
    17821 tatcttccgc aaactgagca aaaagctcgg tggtctgggc ggcggtaaag gcaccggatc
    17881 tggcggtggc agttcatcgc aaggcccgcg cccgcagctt ggcggtcgtg tcgttaccat
    17941 cgcagcggca gcgattgtca ttatctgggc ggccagtggt ttctatacca ttaaagaagc
    18001 cgaacgcggc gtggtaacac gctttggtaa attcagccat ctggttgagc cgggtctgaa
    18061 ctggaaaccg acgtttatcg acgaagtcaa accggtgaac gtggaagccg tgcgtgaact
    18121 ggccgcttct ggtgtgatgc tgacgtcgga cgagaacgta gtgcgcgttg agatgaacgt
    18181 gcagtaccgc gtcaccaatc cggaaaaata tctgtatagc gtgaccagcc cggatgacag
    18241 cctgcgtcag gctaccgaca gcgccctgcg tggagttatc ggtaaataca ccatggaccg
    18301 cattctgacg gaaggtcgta ccgtgattcg tagcgatact cagcgcgaac tggaagagac
    18361 gattcgtccg tatgacatgg gtatcacgct gctggacgtc aacttccagg ctgctcgtcc
    18421 gccggaagaa gtaaaagcgg cgtttgacga tgcgattgcc gcgcgtgaaa acgaacagca
    18481 atacattcgt gaagcagaag cgtattccaa cgaagttcag ccgcgtgcga acggtcaggc
    18541 gcaacgtatc ctcgaagagg cgcgtgcgta caaggcccag accatcctgg aagctcaggg
    18601 tgaagtggcg cgctttgcta aacttctgcc ggaatataaa gccgcgccgg aaattactcg
    18661 cgagcgtctg tatatcgaga cgatggaaaa agtgttgggt aacacccgca aagtgctggt
    18721 taacgataaa ggtggcaacc tgatggttct gccgttagac cagatgctga aaggtggtaa
    18781 cgcccctgcg gcgaagagcg ataacggtgc cagcaatctg ctgcgtctgc cgccagcctc
    18841 ttcctccaca accagtggag caagcaacac gtcgtccacc agtcagggcg atattatgga
    18901 ccaacgccgc gccaacgcgc agcgtaacga ctaccagcgt cagggggaat aacgatgcgt
    18961 aagtcagtta tcgcgattat catcatcgtg ctggtagtgc tttacatgtc tgtctttgtc
    19021 gtcaaagaag gtgagcgcgg tattacgctg cgttttggta aggtactgcg tgacgatgac
    19081 aacaaacctt tggtttatga gcctggtctg catttcaaga taccgttcat tgaaacggtg
    19141 aaaatgctcg acgcacgtat tcagaccatg gacaaccagg ccgaccgctt tgtgaccaaa
    19201 gagaagaaag acctgatcgt cgactcttac atcaaatggc gcatcagcga tttcagccgt
    19261 tactacctgg caacgggtgg tggcgacatt tcgcaagcgg aagtgctgtt gaaacgtaag
    19321 ttctctgacc gtctgcgttc tgaaattggt cgcctggacg tgaaagatat cgtcaccgat
    19381 tcccgtggtc gtctgaccct cgaagtacgt gacgcgctga actccggttc tgcgggtaca
    19441 gaagatgaag ttactacccc ggcagcagat aacgccattg ccgaagcggc agagcgcgta
    19501 acggctgaga cgaagggcaa agttccggtc atcaacccga acagtatggc ggcgctgggt
    19561 attgaagttg tcgatgtgcg tatcaagcag atcaacctgc cgaccgaagt gtctgaagcg
    19621 atctacaacc gtatgcgcgc cgagcgtgaa gcggtagcgc gtcgtcaccg ttcacaaggt
    19681 caggaagaag cggaaaaact gcgcgcgact gccgactatg aagtgaccag aacgctggca
    19741 gaagctgagc gtcagggccg cattatgcgt ggtgaaggcg atgccgaagc agccaaactg
    19801 tttgctgatg cattcagtaa agatccggac ttctacgcat tcatccgtag cctgcgtgct
    19861 tatgagaaca gcttctctgg caatcaggac gtgatggtca tgagcccgga tagcgatttc
    19921 ttccgctaca tgaagacgcc gacttccgca acgcgttaat ataacgactg cggtacaggt
    19981 caataaagcc accgcatcct cagggatgtc ggtggttttc tttttctata aggataatga
    20041 atgaattcga caatctggct ggcgcttgcc ctggttttgg tactggaagg tttagggccg
    20101 atgctttacc cgaaggcatg gaagaagatg atctctgcga tgaccaattt gcccgataat
    20161 attttacgtc gttttggcgg tggacttgtg gttgcgggcg ttgtggtcta ctacatgttg
    20221 aggaaaacga ttggctgaac aaaaaacaga ctgatcgagg tcatttttga gtgcaaaaag
    20281 tgctgtaact ctgaaaaagc gatggtagaa tccattttta agcaaacggt gattttgaaa
    20341 aatgggtaac aacgtcgtcg tactgggcac ccaatggggt gacgaaggta aaggtaagat
    20401 cgtcgatctt ctgactgaac gggctaaata tgttgtacgc taccagggcg gtcacaacgc
    20461 aggccatact ctcgtaatca acggtgaaaa aaccgttctc catcttattc catcaggtat
    20521 tctccgcgag aatgtaacca gcatcatcgg taacggtgtt gtgctgtctc cggccgcgct
    20581 gatgaaagag atgaaagaac tggaagaccg tggcatcccc gttcgtgagc gtctgctgct
    20641 gtctgaagca tgtccgctga tccttgatta tcacgttgct ctggataacg cgcgtgagaa
    20701 agcgcgtggc gcgaaagcga tcggcaccac cggtcgtggt atcgggcctg cttatgaaga
    20761 taaagtggca cgtcgcggtc tgcgtgttgg cgaccttttc gacaaagaaa ccttcgctga
    20821 aaaactgaaa gaagtgatgg aatatcacaa cttccagttg gttaactact acaaagctga
    20881 agcggttgat taccagaaag ttctggatga tacgatggct gttgccgaca tcctgacttc
    20941 tatggtggtt gacgtttctg acctgctcga ccaggcgcgt cagcgtggcg atttcgtcat
    21001 gttcgaaggt gcgcagggta cgctgctgga tatcgaccac ggtacttatc cgtacgtaac
    21061 ttcttccaac accactgctg gtggcgtggc gaccggttcc ggcctgggcc cgcgttatgt
    21121 tgattacgtt ctgggtatcc tcaaagctta ctcaactcgt gtaggtgcag gtcctttccc
    21181 ggccgaactg tttgatgaaa ctggcgagtt cctctgcaag cagggtaacg aattcggtgc
    21241 aactacgggt cgtcgtcgtc gtaccggctg gctggacacc gttgccgttc gtcgtgcggt
    21301 acagctgaac tccctgtctg gcttctgcct gactaaactg gacgttctgg atggcctgaa
    21361 agaggttaaa ctctgcgtgg cttaccgtat gccggatggt cgcgaagtga ctaccactcc
    21421 gctggcagct gacgactgga aaggtgtaga gccgatttac gaaaccatgc cgggctggtc
    21481 tgaatccact ttcggcgtga aagatcgtag cggcctgccg caggcggcgc tgaactacat
    21541 caagcgtatt gaagagctga ccggtgtgcc gatcgatatc atctctaccg gtccggatcg
    21601 tactgaaacc atgattctgc gcgacccgtt cgacgcgtaa ttctggtacg cctggcagat
    21661 attttgcctg ccgggctaac agtgtgatac attgctgtgt cggttaagcc attacgctat
    21721 ccgacacagt gttaaatcct cgcttttttc cttccccgaa ctgaaataaa ttagcgacac
    21781 agcttgtggc tggtttatca tcaatataaa tgtatttttt cccgatttcc cttttgaggt
    21841 tgatgtgcag ttaacgagtt tcactgatta cggattacgt gcgctgatct acatggcgtc
    21901 attgccagaa gggcggatga ccagtatttc tgaagtgact gacgtctacg gcgtctcccg
    21961 taatcatatg gtcaaaataa tcaatcaact tagtcgtgcc ggctacgtga ctgctgttcg
    22021 tggaaaaaat ggcggcattc gcctgggtaa accggcgagt gcgatacgta ttggtgatgt
    22081 ggtgcgcgag ctggagccct tatcgctggt gaattgcagc agtgagtttt gccacattac
    22141 acctgcctgt cggttgaaac aggcgctttc taaggccgtg caaagttttc ttacggaact
    22201 ggataactac acgcttgccg atttggttga agagaatcaa ccgctttata aattattgct
    22261 ggtggagtga cgaaaatctt catcagagat gacaacggag gaaccgagat gtcacaagat
    22321 cctttccagg aacgcgaagc tgaaaaatac gcgaatccca tccctagtcg ggaatttatc
    22381 ctcgaacatt taaccaaacg tgaaaaaccg gccagccgtg atgagctggc ggtagaactg
    22441 cacattgaag gcgaagagca gcttgaaggc ctgcgtcgcc gcctgcgcgc gatggagcgc
    22501 gatggtcaac tggtcttcac tcgtcgtcag tgctatgcgc tgccggaacg cctcgacctg
    22561 gtgaaaggta ccgttattgg tcaccgtgat ggctacggct ttctgcgggt tgaagggcgt
    22621 aaagatgatt tgtatctctc cagcgagcag atgaaaacct gcattcatgg cgatcaggtg
    22681 ctggcgcagc cgctgggtgc tgaccgtaaa ggtcgtcgtg aagcgcgtat tgtccgcgta
    22741 ctggtgccaa aaaccagcca gattgttggt cgctacttta ccgaagcggg cgtcggcttt
    22801 gtggttcctg acgatagccg tctgagcttc gatatcttaa tcccgcccga tcagatcatg
    22861 ggcgcgcgga tgggctttgt ggtcgtagtc gaactgactc agcgtccgac tcgccgcacc
    22921 aaagcggtgg gtaaaatcgt cgaagtgctg ggcgacaata tgggcaccgg catggcggtt
    22981 gatatcgctc tgcgtaccca tgaaattccg tatatctggc cgcaggctgt tgagcaacag
    23041 gttgcggggc tgaaagaaga agtgccggaa gaagcaaaag cgggccgtgt cgatttgcgc
    23101 gatttaccgc tggtcaccat tgatggcgaa gacgcccgtg actttgacga tgcagtttac
    23161 tgcgagaaaa aacgcggcgg cggctggcgt ttatgggtcg cgattgccga cgtcagctac
    23221 tatgtgcgtc cgccaacgcc gctggacaga gaagcgcgta accgtggcac gtcggtgtac
    23281 ttcccttcgc aggttatccc gatgctgccg gaagtgctct ctaacggcct gtgttcgctc
    23341 aacccgcagg tagaccgcct gtgtatggtg tgcgagatga cggtttcgtc gaaaggccgc
    23401 ctgacgggct acaaattcta cgaagcggtg atgagctctc acgcgcgtct gacctacacc
    23461 aaagtctggc atattctgca gggcgatcag gatctgcgcg agcagtacgc cccgctggtt
    23521 aagcatctcg aagagttgca taacctctat aaagtgctgg ataaagcccg tgaagaacgc
    23581 ggtgggatct catttgagag cgaagaagcg aagttcattt tcaacgctga acgccgtatt
    23641 gaacgtatcg aacagaccca gcgtaacgac gcgcacaaat taattgaaga gtgcatgatt
    23701 ctggcgaata tctcggcggc gcgtttcgtt gagaaagcga aagaaccggc actgttccgt
    23761 attcacgaca agccgagcac cgaagcgatt acctctttcc gttcagtgct ggcggagctg
    23821 gggctggagc tgccgggtgg taacaagccg gaaccgcgtg actacgcgga actgctggag
    23881 tcggttgccg accgtcctga tgcagaaatg ctgcaaacca tgctgctacg ctcgatgaaa
    23941 caggcgattt acgatccaga aaaccgtggt cacttcggtc tggcattgca gtcctatgcg
    24001 cactttactt cgccgattcg tcgttatcct gacctgacgc tgcaccgcgc cattaaatat
    24061 ctgctggcga aagagcaggg gcatcagggc aacaccactg aaaccggcgg ctaccattat
    24121 tcgatggaag agatgttgca actgggtcag cactgttcga tggcggaacg tcgtgccgac
    24181 gaagcaacgc gcgatgtcgc tgactggctg aagtgtgact tcatgctcga ccaggtaggt
    24241 aacgtcttta aaggcgtaat ttccagcgtc actggctttg gcttcttcgt ccgtctggac
    24301 gacttgttca ttgatggtct ggtccatgtc tcttcgctgg acaatgacta ctatcgcttt
    24361 gaccaggtag ggcaacgcct gatgggggaa tccagcggcc agacttatcg cctgggcgat
    24421 cgcgtggaag ttcgcgtcga agcggttaat atggacgagc gcaaaatcga ctttagtctg
    24481 atctccagtg aacgcgcacc gcgcaacgtc ggtaaaacgg cgcgcgagaa agcgaaaaaa
    24541 ggcgatgcag gcaaaaaagg cggcaagcgt tgtcaggtcg gtaaaaaggt aaactttgag
    24601 ccagacagcg ccttccgcgg tgagaaaaaa acgaagccga aagcggcgaa gaaagacgcg
    24661 agaaaagcga aaaagccatc ggcgaaaacg cagaaaatag ccgcagcgac caaagcgaag
    24721 cgtgcggcga agaaaaaagt ggcagagtga tcaataccct ctttaaaaga agagggttag
    24781 attgctgaca aaatgcgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta
    24841 cataatcacg caaattcaat atattgcaga gatcatgtag gccggataaa cgtagtgcat
    24901 caggcaaaac gtaaacaacg agtacattaa tgagcgaaat gatttacggc atccacgcag
    24961 tgcaggccct gctggagcgc gcccctgaac gttttcagga agtctttatt ttaaaaggcc
    25021 gtgaagataa acgtctgtta ccgctgattc acgcccttga gtctcagggc gtggttatcc
    25081 agttggcaaa ccgccaatat ctcgacgaga aaagcgacgg tgccgtgcat cagggcatta
    25141 tcgcccgcgt gaagccagga cgtcagtatc aggaaaacga tctgccggat ctgatcgctt
    25201 cgctcgatca accgttcctg ctgatcctcg acggcgtaac cgatccgcac aacctcggcg
    25261 cgtgcctgcg tagtgcggac gccgcaggcg ttcatgcggt gattgtgccg aaagatcgct
    25321 ccgcacagct caacgctacg gcgaaaaaag tagcctgcgg cgcggcagaa agcgttccgc
    25381 tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt gcaggaagag aatatctgga
    25441 tcgtcggtac ggcaggcgag gcggatcata cactctatca gagcaaaatg accggacgcc
    25501 tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg cctgacccgt gaacattgcg
    25561 atgagttgat cagcatccca atggcaggaa gtgtttcttc cctgaacgtt tcggttgcaa
    25621 ccggaatttg cttatttgaa gcggttcgtc agcgcagcta gttcctctga attatgaaaa
    25681 gccatccaga tttggatggt ttt
//
LOCUS       DBBBSB010000056        25659 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.056, whole genome shotgun sequence.
ACCESSION   DBBBSB010000056 DBBBSB010000000
VERSION     DBBBSB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.056"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACIUS0_002234"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACIUS0_002234"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGB0219967.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACIUS0_002235"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACIUS0_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGB0219968.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACIUS0_002236"
     CDS             2630..3391
                     /locus_tag="ACIUS0_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGB0219969.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACIUS0_002237"
     CDS             complement(3442..4326)
                     /locus_tag="ACIUS0_002237"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGB0219970.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACIUS0_002238"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACIUS0_002238"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGB0219971.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACIUS0_002239"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACIUS0_002239"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGB0219972.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACIUS0_002240"
     CDS             6202..6474
                     /locus_tag="ACIUS0_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGB0219973.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACIUS0_002241"
     CDS             6844..7920
                     /locus_tag="ACIUS0_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGB0219974.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACIUS0_002242"
     CDS             7947..9326
                     /locus_tag="ACIUS0_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219975.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACIUS0_002243"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACIUS0_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="ACIUS0_002244"
     CDS             10409..11245
                     /locus_tag="ACIUS0_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGB0219976.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="ACIUS0_002245"
     CDS             11250..12197
                     /locus_tag="ACIUS0_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGB0219977.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="ACIUS0_002246"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="ACIUS0_002246"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGB0219978.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="ACIUS0_002247"
     CDS             13324..14082
                     /locus_tag="ACIUS0_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGB0219979.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="ACIUS0_002248"
     CDS             14200..15558
                     /locus_tag="ACIUS0_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0219980.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACIUS0_002249"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="ACIUS0_002249"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGB0219981.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACIUS0_002250"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="ACIUS0_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGB0219982.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACIUS0_002251"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="ACIUS0_002251"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGB0219983.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="ACIUS0_002252"
     CDS             19503..20054
                     /locus_tag="ACIUS0_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGB0219984.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="ACIUS0_002253"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="ACIUS0_002253"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGB0219985.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="ACIUS0_002254"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="ACIUS0_002254"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGB0219986.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="ACIUS0_002255"
     CDS             23307..24347
                     /locus_tag="ACIUS0_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGB0219987.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACIUS0_002256"
     CDS             complement(24464..25657)
                     /gene="gdhA"
                     /locus_tag="ACIUS0_002256"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGB0219988.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6517 a         5971 c         6625 g         6546 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagta
//
LOCUS       DBBBSB010000057        25269 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.057, whole genome shotgun sequence.
ACCESSION   DBBBSB010000057 DBBBSB010000000
VERSION     DBBBSB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25269
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.057"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            56..1033
                     /gene="ybeQ"
                     /locus_tag="ACIUS0_002257"
     CDS             56..1033
                     /gene="ybeQ"
                     /locus_tag="ACIUS0_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGB0219989.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(1103..1585)
                     /locus_tag="ACIUS0_002258"
     CDS             complement(1103..1585)
                     /locus_tag="ACIUS0_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGB0219990.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            1820..4402
                     /gene="leuS"
                     /locus_tag="ACIUS0_002259"
     CDS             1820..4402
                     /gene="leuS"
                     /locus_tag="ACIUS0_002259"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGB0219991.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4417..4998
                     /gene="lptE"
                     /locus_tag="ACIUS0_002260"
     CDS             4417..4998
                     /gene="lptE"
                     /locus_tag="ACIUS0_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGB0219992.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            4998..6029
                     /gene="holA"
                     /locus_tag="ACIUS0_002261"
     CDS             4998..6029
                     /gene="holA"
                     /locus_tag="ACIUS0_002261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGB0219993.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6031..6672
                     /gene="nadD"
                     /locus_tag="ACIUS0_002262"
     CDS             6031..6672
                     /gene="nadD"
                     /locus_tag="ACIUS0_002262"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGB0219994.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6696..7307
                     /locus_tag="ACIUS0_002263"
     CDS             6696..7307
                     /locus_tag="ACIUS0_002263"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGB0219995.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7567..7884
                     /gene="rsfS"
                     /locus_tag="ACIUS0_002264"
     CDS             7567..7884
                     /gene="rsfS"
                     /locus_tag="ACIUS0_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGB0219996.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7888..8355
                     /gene="rlmH"
                     /locus_tag="ACIUS0_002265"
     CDS             7888..8355
                     /gene="rlmH"
                     /locus_tag="ACIUS0_002265"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGB0219997.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8386..10287
                     /gene="mrdA"
                     /locus_tag="ACIUS0_002266"
     CDS             8386..10287
                     /gene="mrdA"
                     /locus_tag="ACIUS0_002266"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGB0219998.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10290..11402
                     /gene="mrdB"
                     /locus_tag="ACIUS0_002267"
                     /gene_synonym="rodA"
     CDS             10290..11402
                     /gene="mrdB"
                     /locus_tag="ACIUS0_002267"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGB0219999.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11413..12501
                     /gene="rlpA"
                     /locus_tag="ACIUS0_002268"
     CDS             11413..12501
                     /gene="rlpA"
                     /locus_tag="ACIUS0_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGB0220000.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12640..13851
                     /gene="dacA"
                     /locus_tag="ACIUS0_002269"
     CDS             12640..13851
                     /gene="dacA"
                     /locus_tag="ACIUS0_002269"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGB0220001.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13962..14225
                     /gene="ybeD"
                     /locus_tag="ACIUS0_002270"
     CDS             13962..14225
                     /gene="ybeD"
                     /locus_tag="ACIUS0_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGB0220002.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14326..14967
                     /gene="lipB"
                     /locus_tag="ACIUS0_002271"
     CDS             14326..14967
                     /gene="lipB"
                     /locus_tag="ACIUS0_002271"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGB0220003.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15226..16179
                     /locus_tag="ACIUS0_002272"
     CDS             15226..16179
                     /locus_tag="ACIUS0_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGB0220004.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            16388..17353
                     /gene="lipA"
                     /locus_tag="ACIUS0_002273"
     CDS             16388..17353
                     /gene="lipA"
                     /locus_tag="ACIUS0_002273"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGB0220005.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17454..17657)
                     /gene="tatE"
                     /locus_tag="ACIUS0_002274"
     CDS             complement(17454..17657)
                     /gene="tatE"
                     /locus_tag="ACIUS0_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGB0220006.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17786..18574)
                     /locus_tag="ACIUS0_002275"
     CDS             complement(17786..18574)
                     /locus_tag="ACIUS0_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGB0220007.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            18667..19050
                     /gene="crcB"
                     /locus_tag="ACIUS0_002276"
     CDS             18667..19050
                     /gene="crcB"
                     /locus_tag="ACIUS0_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGB0220008.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(19104..19313)
                     /gene="cspE"
                     /locus_tag="ACIUS0_002277"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19104..19313)
                     /gene="cspE"
                     /locus_tag="ACIUS0_002277"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGB0220009.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19488..20048)
                     /gene="pagP"
                     /locus_tag="ACIUS0_002278"
     CDS             complement(19488..20048)
                     /gene="pagP"
                     /locus_tag="ACIUS0_002278"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGB0220010.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20637..22022
                     /gene="dcuC"
                     /locus_tag="ACIUS0_002279"
     CDS             20637..22022
                     /gene="dcuC"
                     /locus_tag="ACIUS0_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGB0220011.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22063..22404)
                     /locus_tag="ACIUS0_002280"
                     /pseudo
     CDS             complement(22063..22404)
                     /locus_tag="ACIUS0_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(22742..23251)
                     /locus_tag="ACIUS0_002281"
     CDS             complement(22742..23251)
                     /locus_tag="ACIUS0_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGB0220012.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            23423..23659
                     /locus_tag="ACIUS0_002282"
     CDS             23423..23659
                     /locus_tag="ACIUS0_002282"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0220013.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            23634..25265
                     /locus_tag="ACIUS0_002283"
                     /pseudo
     CDS             23634..25265
                     /locus_tag="ACIUS0_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         6402 a         6212 c         6306 g         6349 t
ORIGIN      
        1 aaatggaaca cataaaaata taatatattt tatctacaac cataaggaaa tgataatgat
       61 ttttacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
      121 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
      181 ctctccggac gacgaaaagg ccttttactg gctgaagctg gccgctgagc aaggtcattg
      241 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
      301 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
      361 cgcccttggc tggatactgg atcgtggaga agaccccaac tataaagaag cggttacctg
      421 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg gatggatgta
      481 cagaaatggc aacggagtcg cacaagacta tacgctggca tttttctggt acaaacaagc
      541 tgcattacaa ggccatagtt acgcacaaga caatctggcc gatctttata aagacggaga
      601 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
      661 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
      721 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
      781 ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc
      841 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
      901 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
      961 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     1021 agctttactg tagaaataaa aacaagggcc ggggaatttc attctgccca tattctcttt
     1081 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcataac
     1141 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     1201 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     1261 tgaggtcctg gaaaatttcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct
     1321 cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     1381 tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat
     1441 cgatctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     1501 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgtt cagtgacgcg accagttcac
     1561 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     1621 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtac
     1681 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     1741 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     1801 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     1861 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     1921 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     1981 taactacacc atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct
     2041 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     2101 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     2161 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     2221 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata aaaagacttc
     2281 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     2341 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     2401 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     2461 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     2521 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     2581 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     2641 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     2701 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     2761 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     2821 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     2881 gaacatcaaa ccggttatcc tggcggctga cggctctgag ccagatcttt ctcagcaagc
     2941 cctgactgaa aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc
     3001 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     3061 ctaccgcctg cgcgactggg gtgtttctcg ccagcgttac tggggcgcgc cgattccgat
     3121 ggtgacgctg gaagacggta ccgtaatgcc aaccccggac gaccagctgc cggtgatcct
     3181 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     3241 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     3301 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     3361 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     3421 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     3481 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     3541 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     3601 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     3661 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     3721 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     3781 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     3841 gaaactggtg tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     3901 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga
     3961 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     4021 caaactggcg aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact
     4081 gcaggccgtt gtccgtatgc tcaacccgtt caccccgcac atctgcttca cgctgtggca
     4141 ggaactcaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     4201 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     4261 caccgtcccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     4321 ggtagcaaaa tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact
     4381 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacactgttg
     4441 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag
     4501 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     4561 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     4621 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     4681 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
     4741 ttgatccccg gccgtgatat ctacccgatt agcgccaaag tcttccgttc attcttcgat
     4801 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     4861 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     4921 tccgacgaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     4981 accatgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     5041 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     5101 aggacgctgt tcgccaggta gctgcggcac aaggattcga agaacaccac actttttcca
     5161 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     5221 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     5281 aacaacttct cacactcacc ggacttctgc atgacgacct gctattgatc gtccgcggta
     5341 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
     5401 tgcaggtgac ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgtgcgcg
     5461 caaaacagct caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg
     5521 aaggtaacct gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg
     5581 gcaaactgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     5641 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     5701 aactgcgtct ggaaggcagc gagccggtta ttttgttgcg cacattacaa cgtgaactgt
     5761 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     5821 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc
     5881 agccgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     5941 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     6001 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
     6061 acctttgatc cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt
     6121 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     6181 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     6241 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     6301 gagtggcggc aggaacaagg accggacgtg ccgctggcgt ttattattgg tcaggattca
     6361 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     6421 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     6481 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     6541 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     6601 ggtgaatcgt gtgaggattt attgccggaa ccggtattga cttacattaa ccaacaaggc
     6661 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     6721 gcgaaacaca agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg
     6781 cacgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgact
     6841 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc
     6901 gccagctccc cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     6961 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     7021 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     7081 aacgctttat cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc
     7141 atcagggcgt actgagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt
     7201 ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
     7261 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa cgcatgactt ttctgttttt
     7321 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
     7381 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
     7441 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
     7501 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
     7561 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
     7621 ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc
     7681 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
     7741 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
     7801 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
     7861 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
     7921 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
     7981 cccttcgagc tgattgaaat tccggccggc aaacgcggca agaatgcgga catcaagcgc
     8041 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
     8101 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
     8161 aagctggatg gtcgcgacgt aagtctactg attggcgggc ctgaagggtt gtcgcctgcc
     8221 tgtaaagcgg cggctgagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
     8281 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
     8341 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
     8401 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
     8461 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
     8521 cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg
     8581 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc
     8641 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
     8701 cgcagcgtgg tagatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
     8761 tcacaccgtt tcacctctat tccggtgaaa actaacctaa ccgaagtaca agtagctcgc
     8821 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
     8881 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
     8941 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatt
     9001 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
     9061 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
     9121 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
     9181 ctggcgggta gccgcgcagc tgtggttgtt accgaaccgc gtacaggtgg ggtgctggcg
     9241 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
     9301 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
     9361 tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg
     9421 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa
     9481 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
     9541 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
     9601 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
     9661 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
     9721 ggtgacacca ttccggttgg tatcggccag ggttactgga cagcgacccc aatccagatg
     9781 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
     9841 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc
     9901 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
     9961 cctaatggta cggcgaataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct
    10021 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    10081 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    10141 gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc
    10201 cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca
    10261 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    10321 gggataaagt ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca
    10381 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg
    10441 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    10501 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    10561 ctttcggagc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    10621 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    10681 acgtttgccc gccatcgttg aagaatactg gcatcgcgct ggtgctgata tttatgccca
    10741 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    10801 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    10861 tagcggcgtt cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    10921 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    10981 ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt
    11041 cacagcttga atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag
    11101 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    11161 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa
    11221 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    11281 ttgtaggggt tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg
    11341 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    11401 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    11461 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    11521 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    11581 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    11641 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    11701 tctggcgaag ctttcgatcc gacaaagctg acggcggccc atccaacgct tccgatcccc
    11761 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    11821 cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt
    11881 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    11941 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    12001 cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt
    12061 cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    12121 agcggtttcc tcggcgctcc aacgacctta gcgcctggtg tactggaagg cagcgaaccg
    12181 acgcctgcgc cacagcccgt tgttacagct tcgtcgacaa cgcctgcaac ctcgcctgca
    12241 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    12301 gccgtaagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    12361 gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc
    12421 agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    12481 tttattacta ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    12541 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    12601 cactttttta attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct
    12661 cgtatcatga agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca
    12721 catgccgatg acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag
    12781 tcctacatcc tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc
    12841 cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg
    12901 aaagccggta aatttaaaga aactgattta gtcactatcg gcaacgacgc atgggccacc
    12961 ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg
    13021 gtttctcagc tgatccgcgg tattaacctg caatcgggta acgatgcttg tgtcgccatg
    13081 gccgattttg ccgctggtag ccaggacgct tttgttggct tgatgaacag ctacgttaac
    13141 gcactgggcc tgaaaaatac ccacttccag acggtacatg gtctggatgc tgatggtcag
    13201 tacagctccg cgcgagatat ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat
    13261 gaatactcga tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt
    13321 aacggcctgt tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac
    13381 aaagcaggtt acaatcttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg
    13441 gtgatgggtg gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg
    13501 ggcttccgtt tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa
    13561 ccggtttggt ttagtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg
    13621 accattccgc gtggtcgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa
    13681 ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc
    13741 aaaacgatcg agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc
    13801 ggcaaaatca ttgattacat taaattaatg ttccatcact ggtttggtta aaaattgaac
    13861 acttgaaagt gtaatttccg tccccatata ctaagcatca gtaaaaaaac tcccgccttc
    13921 tggcgggagt tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg
    13981 aactgcttga attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc
    14041 tggttgatca ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg
    14101 taaaaccaag cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata
    14161 tcgagcaggt tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc
    14221 tgtaactcgc ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga
    14281 tatacttttc ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa
    14341 attcttgtcc gccagctcgg tcttcagcct tacgagccaa tctcccgggc tatgcatgaa
    14401 ttcaccgata cccgcgataa tagtaccctt gatgaaatct ggctggtcga gcactatccg
    14461 gtattcaccc aaggtcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg
    14521 gtgatccaga gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca acaggtgatg
    14581 tatgtgttgc ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt
    14641 gagcaaacag tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac
    14701 gcgcctggtg tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg aattcgacgc
    14761 ggttgttcat tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt
    14821 attaatcctt gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa
    14881 gcgacgacta ataatattgc tccacgttta ctggaaaata ttttagcgct actaaacaat
    14941 ccggacttcg aatatattac cgcttaattc catacatcaa tggcccaatt tacactgggt
    15001 cattactcat aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat
    15061 cacttgcaga aatgcggtgc cacgccttgc tccgtaactc accccattga ttaacctatt
    15121 gaaatggttg attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta
    15181 attttttaaa tattattttt ccatgaataa aattggagta agtgcgtgga tagtaataat
    15241 caaattgaac cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc
    15301 ttacgaaata ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg
    15361 atcgttaatg cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag
    15421 aaactgcgcg ttatattccc tgacccattg tttattcgca aaggccaggg tgtcactcct
    15481 accgcatttg cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc
    15541 gcgctggata tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc
    15601 tcggtcggag ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag
    15661 cttttactgc gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc
    15721 gatctcatca tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc
    15781 accgacaata tggtgttaat ttgccgcaag ggaaatccac tactctcttt agaagatgac
    15841 agagagacta tcgacaacgc tgcgcatgta cttctgttac cggaagggca aaatttcagc
    15901 ggtctgcggc agagagttca agagatgttt ccggaccggc aaatcaattt caccagctac
    15961 aacattttga caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc
    16021 cgtttttata acctgtttag tcgttgctgg ccgctggaaa aactgccttt tccgtcctta
    16081 aatgaggagc aaatagattt ctctatccac tacaacaaat tcatcctgcg tgatcccatc
    16141 ctgcacggag taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
    16201 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
    16261 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
    16321 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
    16381 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
    16441 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
    16501 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
    16561 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
    16621 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
    16681 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
    16741 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
    16801 tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga
    16861 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gaatggatcg
    16921 tgctctggat attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt
    16981 accgcgtatt taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct
    17041 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggtct
    17101 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
    17161 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
    17221 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
    17281 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaaggaat
    17341 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
    17401 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
    17461 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
    17521 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
    17581 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
    17641 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
    17701 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
    17761 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
    17821 ttaagatggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
    17881 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
    17941 tttgcccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
    18001 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg
    18061 cggcaggaag taccaggatt tcagctccct gtaaagcctg tgccagcgcc agctctggaa
    18121 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
    18181 ctatttcatt accagcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat
    18241 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
    18301 cgcgccccgg cgttgaagga agatgaatcg tcagaattgt cgtcatcatg ttacgtttac
    18361 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
    18421 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
    18481 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
    18541 atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa
    18601 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
    18661 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtagcg
    18721 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
    18781 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
    18841 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
    18901 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
    18961 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
    19021 tggctgtttt cggcctcaac cgtacactaa agaccaaaaa aaacccgctg attaagcggg
    19081 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
    19141 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
    19201 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
    19261 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
    19321 acctttacat gaaaaataga cacaaatgct gtgtcggtta tcagtacacc aattgtggta
    19381 cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttgc
    19441 ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgtctca aaactgaaag
    19501 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
    19561 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
    19621 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
    19681 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
    19741 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
    19801 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
    19861 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
    19921 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa
    19981 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
    20041 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
    20101 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
    20161 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg
    20221 cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg
    20281 aaaaattatt ttattttttt aacgagatac aacaatcatc ttaacgaagt atataatata
    20341 tatcgtcaac ccatgctcat attagattat tttttgagcg cacagccgca gctaacacca
    20401 ttgcaattaa caaatttgca tcaatccacc atcaatttac acacattatt atgtgataat
    20461 tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact tcaatgtctt
    20521 aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg catatttttt
    20581 ccttaagctt taggaagttt ttattattta ctttgggcct ggagacagga aaaattatgc
    20641 tgacattcat tgaactcctt attggggttg tggttattgt gggtgtggct cgctacatca
    20701 ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg cctgttattg ctgattatca
    20761 gtgccattat ggggcacaaa gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca
    20821 cggatatcgt tgaatacgtt aaaatattac taatgagccg cggcggcgac ctcggcatga
    20881 tgattatgat gctgtgtggc tttgccgctt acatgaccca tatcggcgcg aatgatatgg
    20941 tggtcaagct ggcgtcaaaa ccattgcagt atattaactc cccttacctg ctgatgattg
    21001 ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc ttccgcaacc ggtctggggg
    21061 ttttgctgat ggcaaccctg tttccggtga tggtaaacgt tggtatcagt cgtggcgcag
    21121 cagctgccat ttgtgcctcc ccggcggcga ttattctcgc accgacttca ggggatgtgg
    21181 tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc
    21241 ctatctcaat tgctgcaatt atcggcatgg cgatcgccca cttcttctgg caacgttatc
    21301 tggataaaaa agagcacatc tctcatgaaa tgttagatgt cagtgaaatc accaccactg
    21361 cccccgcgtt ttatgccatt ttgccgttca cgccgatcat cggtgtactg atttttgacg
    21421 gtaaatgggg tccgcaatta cacatcatca ctattctggt gatttgtatg ctgattgcct
    21481 ccattctgga gttcctccgc agctttaata cccagaaagt tttctctggt ctggaagtgg
    21541 cttatcgcgg gatggcagat gcgtttgcta acgtggtgat gctgctggtt gccgctgggg
    21601 tattcgctca ggggcttagc accatcggct ttattcaaag tctgatttct atcgctacct
    21661 cgtttggttc ggcgagtatc atcctgatgc tggtattggt gattctgacc atgctggcgg
    21721 cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac
    21781 tggcgcactc ttccggcatt aacccggcgt atttgactat cccgatgctg caggcgtcaa
    21841 acctgggtcg taccctatca cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga
    21901 aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc ggtgcttgtt gggctagtga
    21961 ttgttatcgt tgctacagag ctgatggtgc caggaacggc agcagcggtc acaggcaagt
    22021 aaatagtaat gccggaggca agtttcctcc ggctttatca tgtcacccac tgtggtatat
    22081 gcgttgtggt ctgccaactt tgccgtgaac aatttcagca ataatcagat ggcggctggc
    22141 gcaatattca agataacgcc tggcagtggt gcggctgatg gtcagtgcct gcgccaccgt
    22201 ttctgccgta tgttgcacac caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa
    22261 ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg cgagcatagg cattaaacat
    22321 ctcatcaatt tgcttctggc tggcgctatc aatactttcc agcatatgtt tacgctggcg
    22381 gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg aagtacgaaa aaaccgggag
    22441 aacattggat tattcgggat ctgatgggat tagatttggt ggggcttgca agcctgtagt
    22501 acaaatttta ggcgttaacc aatgaaacgc gaaagatagt aaaaaattgc ttttgtttca
    22561 ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa tcatagcttc aaaaaacgtt
    22621 caaaaccgat aacaaccagg ctgtcatcaa atgactcata tcacaaatca gctcatgccg
    22681 tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg gctcatggtt
    22741 gttatacggc ttttttcgca tcttgtggtt ccaacaaagg agctacaggc tcttcctgtt
    22801 tatgaacacc atacattgac aaaatctctt ggcgagtttc tggaggtaat ttatgcagcg
    22861 ccaaagctgt ttgtagtgtt tggcgatcaa tacccagcgt tgccaatagg gtgtcaacgg
    22921 agatggaggg gcgttcttgc attaatccgg aaagcccata tttagccatg tttagctcta
    22981 tctctcggac ttcattttct cctagtttat tgattaacag ggagaacatt ttcaatgtat
    23041 cagcattcat ttcttcgata gcaatttttt gcatagccgg tattgccggg atatattcaa
    23101 ccccaggccc gcgacgccct tcttgtgaac gcttcggcca tccttctttc tttgctcgtt
    23161 tatgcacccc ctgtattgtc ccaggcattc catccagttc agtaatttgt ttgggtgtca
    23221 tccactggtt tatctcattt gcgcttttca taaactaagt ttaccagatt tatctatata
    23281 aaaatcaatg gattacaaaa taaaccacat taactgatat aaaccggttt ggtttatttg
    23341 gtaatgtata aaacagataa tcaagacgaa ttatttgttt tggtaaccct ttatcatacg
    23401 caatatggtt ggtgtgcgaa agatgagtaa cgattggcat cgggccgacg tgattgcggc
    23461 cctcaaaaaa acaggaaccc ctctggcaaa attgtccaga cgttcaggtt attcaaccgg
    23521 gacgcttgca aatgcattag tcaggccatg gcctaaaggt gaacgcatta ttgctgacgc
    23581 cttaaacatg aaacctgagg aaatttggcc tagtagatat ctaagggatc acaatgagag
    23641 agtggatatc agcaattgaa tgcatgggtt tatctggttt acctggttca ttaatggggg
    23701 ttcacaaaag agctcagaga gaaggatggc tcaagagaag aagagaagga attagagggg
    23761 ttgcttacga ataccatata tcttctcttc ctaacattgc tcgagctgaa gttttgctcc
    23821 aacaaggaaa atttaaaacc agtcagggat gttttgaaat cgcccgcccc acgctggaag
    23881 ctcatgatta tgatcgtgag gcactatgga gcaaatggga taaagccagc gattcccagc
    23941 gcagcctggc tgaaaaatgg ttgccctcaa ttcaggccac agacgaaatg ctgaaccagg
    24001 ggatttcaac gaaaacggct tttgcgaccg ttgcagggca ttaccaggtc agcgcatcca
    24061 ctttgcggga caagtattac caggtacaga agtttgcgaa gcctgactgg gcggctgcac
    24121 ttgttgatgg acgtggagca tcccgtcgca atgttcacaa aagtgaattt gacgaggatg
    24181 cctggcagtt tctgattgca gattatctgc gaccggaaaa acccgccttc cgcaaatgtt
    24241 atgagcgtct ggaactggca gcccgcgagc atggctggag tattccctcc cgtgccacgg
    24301 cctttcgccg gattcagcaa ctggacgagg caatggttgt tgcctgtcgt gaaggtgaac
    24361 atgcactgat gcatctgata ccggcacagc agcgaactgt ggaacacctg gacgccatgc
    24421 agtggatcaa cggcgacggt tatctgcata acgtctttgt acgctggttt aacggtgatg
    24481 tgatccgtcc gaaaacatgg ttctggcagg atgtgaaaac ccgaaaaatt ctgggctggc
    24541 gctgcgatgt gagcgagaac attgattcaa ttcgcctctc gttcatggat gttgtgactc
    24601 gctacagtat cccggaggat tttcacatca ccattgataa cacccgtggt gctgcgaata
    24661 aatggctgac gggaggcgcg cccaatcgct accgctttaa ggtaaaagag gacgatccaa
    24721 aaggactgtt tttactgatg ggtgcgaaaa tgcactggac aagcgttgtt gccggtaaag
    24781 gctggggcca ggcaaaacct gttgaacgtg ctttcggtgt tggtgggctt gaggaatacg
    24841 ttgataagca tccggcactg gctggcgcat atacggggcc aaatccgcag gcaaaacctg
    24901 ataactatgg cgaccacgct gttgatgcag agctgtttct gaaaaccttt gccgaaggtg
    24961 tggcgatgtt caatgccaga acaggccgtg aaacagaaat gtgcgggggc aaactctcgt
    25021 ttgatgatgt tttcgagcgt gaatacgcca gaacgattgt gcgtaagcca accgaagaac
    25081 aaaaacggat gctgttactg cctgccgagg cggtgaacgt ttcacgcaaa ggcgagttta
    25141 cgcttaaagt tggcggctcc cttaaaggcg cgaaaaacgt ttattacaac atggcattaa
    25201 tgaatgccgg agtgaaaaaa gttgtggtca gatttgatcc acagcagtta cacagcacgg
    25261 tttattgag
//
LOCUS       DBBBSB010000058        24677 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.058, whole genome shotgun sequence.
ACCESSION   DBBBSB010000058 DBBBSB010000000
VERSION     DBBBSB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24677
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.058"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(15..91)
                     /locus_tag="ACIUS0_002284"
     tRNA            complement(15..91)
                     /locus_tag="ACIUS0_002284"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Arg,seq:acg)
     gene            complement(95..187)
                     /locus_tag="ACIUS0_002285"
     tRNA            complement(95..187)
                     /locus_tag="ACIUS0_002285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Ser,seq:gct)
     gene            complement(503..688)
                     /gene="csrA"
                     /locus_tag="ACIUS0_002286"
     CDS             complement(503..688)
                     /gene="csrA"
                     /locus_tag="ACIUS0_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGB0220014.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(923..3553)
                     /gene="alaS"
                     /locus_tag="ACIUS0_002287"
     CDS             complement(923..3553)
                     /gene="alaS"
                     /locus_tag="ACIUS0_002287"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGB0220015.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3681..4181)
                     /gene="recX"
                     /locus_tag="ACIUS0_002288"
     CDS             complement(3681..4181)
                     /gene="recX"
                     /locus_tag="ACIUS0_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGB0220016.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4424..5485)
                     /gene="recA"
                     /locus_tag="ACIUS0_002289"
     CDS             complement(4424..5485)
                     /gene="recA"
                     /locus_tag="ACIUS0_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGB0220017.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5565..6062)
                     /gene="pncC"
                     /locus_tag="ACIUS0_002290"
     CDS             complement(5565..6062)
                     /gene="pncC"
                     /locus_tag="ACIUS0_002290"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGB0220018.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6207..7292)
                     /gene="mltB"
                     /locus_tag="ACIUS0_002291"
     CDS             complement(6207..7292)
                     /gene="mltB"
                     /locus_tag="ACIUS0_002291"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGB0220019.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7548..8110
                     /gene="srlA"
                     /locus_tag="ACIUS0_002292"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7548..8110
                     /gene="srlA"
                     /locus_tag="ACIUS0_002292"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8107..9066
                     /gene="srlE"
                     /locus_tag="ACIUS0_002293"
                     /gene_synonym="gutE"
     CDS             8107..9066
                     /gene="srlE"
                     /locus_tag="ACIUS0_002293"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGB0220020.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9077..9448
                     /gene="srlB"
                     /locus_tag="ACIUS0_002294"
                     /gene_synonym="gutB"
     CDS             9077..9448
                     /gene="srlB"
                     /locus_tag="ACIUS0_002294"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGB0220021.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9452..10231
                     /gene="srlD"
                     /locus_tag="ACIUS0_002295"
     CDS             9452..10231
                     /gene="srlD"
                     /locus_tag="ACIUS0_002295"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGB0220022.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10336..10695
                     /gene="gutM"
                     /locus_tag="ACIUS0_002296"
     CDS             10336..10695
                     /gene="gutM"
                     /locus_tag="ACIUS0_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGB0220023.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10762..11535
                     /gene="srlR"
                     /locus_tag="ACIUS0_002297"
                     /gene_synonym="gutR"
     CDS             10762..11535
                     /gene="srlR"
                     /locus_tag="ACIUS0_002297"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGB0220024.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11528..12493
                     /gene="gutQ"
                     /locus_tag="ACIUS0_002298"
     CDS             11528..12493
                     /gene="gutQ"
                     /locus_tag="ACIUS0_002298"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGB0220025.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12490..14004)
                     /gene="norR"
                     /locus_tag="ACIUS0_002299"
     CDS             complement(12490..14004)
                     /gene="norR"
                     /locus_tag="ACIUS0_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGB0220026.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14191..15630
                     /gene="norV"
                     /locus_tag="ACIUS0_002300"
     CDS             14191..15630
                     /gene="norV"
                     /locus_tag="ACIUS0_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGB0220027.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15627..16760
                     /gene="norW"
                     /locus_tag="ACIUS0_002301"
     CDS             15627..16760
                     /gene="norW"
                     /locus_tag="ACIUS0_002301"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGB0220028.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLCLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16888..19140)
                     /gene="hypF"
                     /locus_tag="ACIUS0_002302"
     CDS             complement(16888..19140)
                     /gene="hypF"
                     /locus_tag="ACIUS0_002302"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGB0220029.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSILARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19293..19820)
                     /gene="hydN"
                     /locus_tag="ACIUS0_002303"
     CDS             complement(19293..19820)
                     /gene="hydN"
                     /locus_tag="ACIUS0_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGB0220030.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19969..20979)
                     /gene="ascG"
                     /locus_tag="ACIUS0_002304"
     CDS             complement(19969..20979)
                     /gene="ascG"
                     /locus_tag="ACIUS0_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGB0220031.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(21041..21112)
                     /locus_tag="ACIUS0_002305"
                     /pseudo
     CDS             complement(21041..21112)
                     /locus_tag="ACIUS0_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21122..22831)
                     /gene="hycE"
                     /locus_tag="ACIUS0_002306"
     CDS             complement(21122..22831)
                     /gene="hycE"
                     /locus_tag="ACIUS0_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGB0220032.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22849..23772)
                     /gene="hycD"
                     /locus_tag="ACIUS0_002307"
     CDS             complement(22849..23772)
                     /gene="hycD"
                     /locus_tag="ACIUS0_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGB0220033.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23775..>24677)
                     /locus_tag="ACIUS0_002308"
     CDS             complement(23775..>24677)
                     /locus_tag="ACIUS0_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HGB0220034.1"
                     /translation="GIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGH
                     RDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVAR
                     LLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAIC
                     CVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRL
                     PSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGS
                     ALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5822 a         6939 c         6331 g         5585 t
ORIGIN      
        1 tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
       61 cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc
      121 cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca
      181 cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcatcgctgc
      241 gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca cacttttatc
      301 gtttgcacac ttcacgctca attagtctgt aaaagcgtca aaaagagtgt tttatctaca
      361 gaaaaacgga ggtctggcag atagcagtaa tgcaaaaaaa tggagactta agttgaatga
      421 acgggagtaa agcgaaaaga ctatagagtg aaggagaaat tttgagggtg cgtctcaccg
      481 ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac
      541 gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta
      601 cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct
      661 caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg
      721 tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa tgacaaacac
      781 attacatgta agcagtcatg gcattacatt ctgttaaacc taagtttagc cgatatacac
      841 aacttcaacc tgactttatc gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc
      901 acggcattgc ctgacgctta tattattgca atttcgcgct gacccagcct ttcacactgg
      961 ctaacgctgc aggtaaggcc gcagcatccg taccaccggc ttgcgccatg tcaggacgtc
     1021 caccaccctt gccgcccacc tgctgagcga ccataccaat cagttcccct gctttcacac
     1081 gatctgtgac gtccttagat acgcctgcaa tcagagaaac cttaccttcg gctaccgttg
     1141 ccagcacgat aattgtcgac cccagctgat tttttaaatc gtcaaccatg gtacgcaaca
     1201 ttttcggctc aacaccgcta agctcgctaa ccaacagctt aacaccatta acatcaattg
     1261 ccttactgga aagatttgcg ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt
     1321 ctttttctaa ctgacgcgta cgttccagta ctgagcgcac tttatccgcc agattattgc
     1381 tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg
     1441 tgatagcgcc ttctccggtt accgcttcga tacgacgaac gcctgcagca gtacccgatt
     1501 cagagatgat gcggaacaga ccaatatcac cagtgcggct ggcgtgagta ccgccacaca
     1561 gctcggtgga gaaatcgccc atgctcagca cgcgtacacg ctcatcatac ttctcgccga
     1621 acagcgccat cgcacctttc gctttcgccg cttcgagatt catgatgttg gtttcgatcg
     1681 gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac cgcacgaatc tcttccggtt
     1741 tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt
     1801 tctgcgatac atgagtcccc agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg
     1861 agtgattcag acgaatacgg gcgcgacgag cctcatcaat atcagcctgc acagcgtcgc
     1921 ccactttcag agaacccgca gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct
     1981 gcgtatcttc caccgcgaag gagaagttag cgcctttcag ttcgccttta tcaccaacct
     2041 gaccgccgga ttccgcatag aatggcgttt gatccagcac gaccacagct tcctggccgg
     2101 cattgatggc atcaaccgct ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt
     2161 ccagatggtc atagccttta aattcagatg cactatcaac acggatcatt gcgttgtaat
     2221 cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt gctgcttcaa
     2281 aaccagcttc gtcaactttg atgttgcgct cacgacaaac atcagccgtc aggtcaaccg
     2341 ggaagccata ggtgtcgtac agacggaaag ccgtttcacc atccagcgta tcaccagaaa
     2401 gttttgccag ctcttcatcc agcaacgcca gaccgcgctc cagagtacgg gcaaactgct
     2461 cttcttcagt cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg
     2521 cagagcccat aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc
     2581 cgagcatatt gccgtgacgc actgcgcgac gaatgatacg acgcagtaca taaccacggt
     2641 tttcattgga cggcattacg ccatccgcga tcaggaacgc acaagaacga atgtggtcag
     2701 cgattacgcg cagcgattta ttgctcagat cggtcgcgcc agtgactttc gctaccgcct
     2761 ggatcaacgt gcggaacagg tcgatgtcat agttagagtt aacgtgttgc agcaccgcag
     2821 caatacgctc cagacccata ccggtatcta cagacggctt cggcagcggt tccatcgtgc
     2881 catctgcctg gcggttgaac tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc
     2941 cgtcttcttc cggacttccc ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga
     3001 tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg tcagatgcgt
     3061 atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc ccgacttctt
     3121 tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac agacgctctt
     3181 tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga atggcatcgt
     3241 gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg
     3301 cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg
     3361 aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc ttgaactggt
     3421 tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg gagctgctgg
     3481 caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg
     3541 tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg
     3601 tgtcttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg
     3661 aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc
     3721 atcagatagc cacgatagag cagaaaacgc tggatcttaa ctttttctga aaagacagtt
     3781 ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg
     3841 tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc
     3901 tgacgaatac gtgcaggtcc ataccctttg cggctacggc tggcgataaa gcgcgcaaca
     3961 aatcggctgt catcgagata gccatgttca tggcaccagg caataacgcg ctcgtaatct
     4021 tctgccgtag catcaatctc ttctgggcca tttttgccca taatcggtgc cgcgagttta
     4081 cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac
     4141 aggcgagcat atgccgggcg acgggatgtt gattctgtca tggcatatcc ttacaactta
     4201 aaaaagcaaa agggccgcag atgcgcccca ggttttgctg ataaacagat ttaatagtca
     4261 atgagtatgc agaatgttcc cgcaggccca attcgttaga ggatcgaggt gagagtaatt
     4321 tttcacgcca gtgctggcct gttcccctca ccctaaccct ctccccaaag gggcgagggg
     4381 accgtccgtg cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag
     4441 tttctgctac tccttcgctg tcatctacag agaaatccgg cgttgagttc gggttgctca
     4501 gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc
     4561 aggcagttgc attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc
     4621 ctgctttctc gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga
     4681 taccttcgcc gtagaggatc tgaaattcag cctgtttaaa cggtgcagcg attttgttct
     4741 tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga
     4801 tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg
     4861 tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca
     4921 gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg
     4981 ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg
     5041 ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga
     5101 tttccagtgc ctgctcgccg gtatccggct gggagcacag caggttatcg atatcgacgc
     5161 ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt
     5221 taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt
     5281 ccggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat
     5341 ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc
     5401 gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt
     5461 tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt
     5521 caatatgttc tgttgaaaca attatactgt atgctcatac agtatcaagt gttttgtaga
     5581 aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac cgcatcacgg
     5641 tcgccgctga agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac
     5701 cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata
     5761 gacacggcat aatcagcacg agccgctttc agtgcaccta tcgccatttc caccacgacg
     5821 ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat catctgcgct
     5881 ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata
     5941 tcggtaatca ctttcgctac ccaaccaccg gtacaagact cggcagttgt tacggttgcg
     6001 ccacgggctt tcagcgcctg cccaacctgt tcgcttaact gcatcagttc actgtcagtc
     6061 atcacaacct ctgtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga
     6121 tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaggga
     6181 agctcacgcc cccctcttgt aaatagctac tgtactcgcg ccagcgccac ggcttgtcct
     6241 aactgccaga ccgccattgc gtaatgggtg ctgtggttgt aacgggtgat ggtgtagaag
     6301 ttcggcagac cgtaccagta ctggtagccg gtgccaacat ccagacgcag caggctggct
     6361 tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg cggcggcaag ctgcgaaatg
     6421 ctgtacttag ttttgaaacc atttggcaag cctggagcct gaccgtttgc cattatcgcg
     6481 acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct accgatcgca
     6541 tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg
     6601 taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg
     6661 agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg tttccagctc gccagagaaa
     6721 tactccgcgc ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt
     6781 ttccccatca cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc
     6841 ggtacgccat acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc
     6901 acaccgttct gcacgttgtc cggcgtaata aattttttgc gataacgcag ccatgcgccg
     6961 ttcggacctg atggcggttt caccgatgtg gttggtgcct ggttatccat cagccgcagt
     7021 accgaatcca gacgcttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg
     7081 tgtttgttca ccattttgtc gatgaactgc tgggcattcg ggttattagc gaaatcgccg
     7141 cccatctgca tcacattgtg ctgcggctca agcaggaagc cgccagacgg cgttccggtg
     7201 gtcgtttcag tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg
     7261 ggaagcaatg ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag
     7321 gggcaagtat ggtaaagcat cacaccccgc acaaggaagc ggtagtcact gcccgatacg
     7381 gactttacat aactcaactc attcccctcg ctatactttt attcaaactt tcaaattaaa
     7441 atatttatct ttcattttac gatcaaaata acacttttaa acctttcaac ctgattagat
     7501 taggttgccg tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca
     7561 ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg
     7621 ggatggtgac cggcattctt ccgctgttga ttagcctgca ggttatcatg aacgcactga
     7681 ttaattttat cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg
     7741 tttcccgtta cctactgtta ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct
     7801 taagcctggg tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcggcctctt
     7861 atagctgcca ctcaatgaat ggcctcttcc cccatatcaa ccctgcgaac tgtttgttta
     7921 tcttggcatt gccagcggtc tgacaacgct gaacctgcca cttggcccac tggcggtgag
     7981 ttatctgctg gttggtctgg tcaccaattt cttccgcggc tgggtgaccg atctgaccac
     8041 cgccattttt gagaaaaaga tgggcattca acttgaacaa aaagttcacc tggcaggagc
     8101 aacatcatga cgcgtattcg gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag
     8161 ctggaagcca cgccgggcaa aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt
     8221 gttgacaaac tggcacaact tactggctgg caggctattg acggatttaa agaaggtgaa
     8281 cccgcggagg cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc
     8341 atctatccga aacgacgtat tcccaccatt aatatccact cgacgggcaa gtccggtccg
     8401 ctggcgcagt acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact
     8461 gtagtgggtg atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagtaag
     8521 aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc
     8581 gttgccgtgt tgtttcagtc tggtcgtgac accatcgaca ctgtattaaa aaccattctg
     8641 ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta tggcttctgg ccttggtgac
     8701 tggattgccc acggtcttgc tccgctagcg agccatccac tgggtctggt catgctggcg
     8761 ctcatctgct ccttcccact gctttcacct ttcctcggcc caggcgcagt tatcgcacag
     8821 gttatcggcg tattgattgg cgtgcagatt ggtctcggca atattccgcc gcatctggct
     8881 ttaccggcac tgtttgccat caacgcgcag gcggcttgcg acttcatccc ggtcggtttg
     8941 tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt actggtgagc
     9001 cgctttttaa ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg ttttatctat
     9061 caatagaggc tgaaacatga ccgttattta tcagaccacc atcacccgta tcggcgcgag
     9121 tgccactgac gccctcagcg accagatgct catcaccttt cgtgaaggcg cgccagcgga
     9181 cctcgaagag tattgcttca ttcattgcca cggcgagttg aaaggtgcac tccatcccgg
     9241 tttgcaattt tcactcgggc agcatcgcta tccggtgacc gctgttggca gcgtggcgga
     9301 agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga
     9361 atttccgggc actgtccatg tggcaggccc tgtacccgac gatatcgcgc cgggatcggt
     9421 tttgaagttt gaatctgtta aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg
     9481 tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc
     9541 ggttgtcgat attcagagcg acaaagccgc aaatgtggca caagaaatta acgccgaata
     9601 tggtgaaggt atggcgtacg gttttggtgc tgacgccact agcgagcaaa gcgttctggc
     9661 gctctctcgt ggggtagatg aaatctttgg tcgcgtggat ttgctggtct atagcgccgg
     9721 aatagcaaaa gcagccttta tcagcgactt ccagctcggc gattttgacc gttcgctaca
     9781 agtgaatctg gtgggttatt tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga
     9841 cgggattcag ggtcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca
     9901 caactctggc tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat cactggcgct
     9961 ggatctggcg gagtacggca ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc
    10021 gcggatgttc cagtcactgt tgccacaata cgcgaccaag ctgggtatca aaccggatca
    10081 agtcgagcag tattacatcg acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt
    10141 gctgaatatg ctgctgttct acgccagtcc taaggcgtcg tactgcaccg gacagtcgat
    10201 caatgtcacc ggcggtcagg tgatgttctg atcaacagcg gagatccgtt aaggatctcc
    10261 gtgagactat agaatgcctg atgcgctacg ctcatcaggc atacaggact tccgccacta
    10321 cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg
    10381 caactggcct taggcggctg gcaaatttct cgttttaacc gtgccttcga cacactatgc
    10441 cagcaagggc gggttggcgt ggaccgttcc agcgggcgct ttaaaccgcg ggtcgtggtc
    10501 gccatcgcgc tggacgatca gcagtgcatc gtcgacacct tgtttatgaa aggactgact
    10561 gtcttcgccc gaccgcaaaa aattcccgca attaccggca tgcatgcggg tgatttacag
    10621 cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt ggcgcttaaa
    10681 ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc attttgaaac
    10741 ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aggccgccat tctggagtat
    10801 ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc aatactttga caccacaggc
    10861 acaaccattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat tcgtacttat
    10921 ggcggagtgg tgttgaataa agaggaatcc gatccgccta tcgatcataa aacgctcatc
    10981 aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca tgatggcgat
    11041 tcgatcattc ttgatgctgg cagtaccgtt ttgcagattg ttcccctgct ctcgcgcttt
    11101 aataacatca cggtgatgac caacagcctg catatcgtca atgcgctatc cgaactggat
    11161 aacgaacaaa ctatcctgat gccaggcgga acatttcgca aaaaatcggc ctcatttcac
    11221 gggcaactgg cagagaatgc cttcgagcat ttcaccttcg ataaattgtt tatgggcacc
    11281 gacggcatcg atctcaatgc gggcgtaacc acgttcaacg aggtttatac cgtcagtaag
    11341 gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt
    11401 aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctaattac cgacgcaggt
    11461 atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat cataaccgga
    11521 gagagcaatg agtgaagcac tactgaacac gggacgtcag acgttaatgc tggagttgca
    11581 ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg ccaatatcat
    11641 cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc acattggtaa
    11701 gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct ttttttgtcc atccggcaga
    11761 agcactgcac ggcgatctgg ggatgattga aagccgcgat gtgatgctgt ttatctctta
    11821 ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagata aatctatcgc
    11881 gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcga aagcggtgct
    11941 ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct ccagcaccgt
    12001 caataccctg atgatgggtg acgcgctggc gatggcggtc atgcaggcgc gcggttttaa
    12061 tgaagaagat tttgcccgct cccacccagc tggggcactg ggcgctcgct tgctgaataa
    12121 agtgcatcat ctgatgcgtc gtgatgacgc tatcccacag gtagcgttaa ccgccagcgt
    12181 gatggatgcg atgctggaac tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc
    12241 tcaacaacag gtacaaggcg tctttaccga cggcgattta cgtcgctggc tggtaggcgg
    12301 cggcgcactc accacgccag ttaatgaagc gatgacgaca ggcggcacca cattacaggc
    12361 acaaagtcgc gccatcgacg ccaaagaagt tctgatgaaa cgcaaaatca ctgccgcacc
    12421 agtggtagat gaaaacggca aactcaccgg cgcaattaac ctgcaggatt tctatcaggc
    12481 cgggattatt taatccttca atcccagacg tttcgccagc cgatgcaggt tggcgacgtc
    12541 agtttccagc attcgcgcac aggctgccca gttgtgatga ttttgtgcca gtgcctggcg
    12601 aatagtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac
    12661 cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag
    12721 aatcacttca tcgccgttgc gggtggctct cgccagcact accgcccgat gaatagcatg
    12781 ttccagttcg cgcacgtttc ccggaaaact gtagtgttgc agtaaatttc gcgccccggc
    12841 acttaatacc acgcgggaga gcccctgccg caaacgacac tgctcgcaga aataccccgc
    12901 cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg gaaacacgct
    12961 caggcgatga aacaaatcgg cgcggaatcg ccctgccagc acctcttcgc gtaagtcgcg
    13021 gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg
    13081 aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg acaactcgcc
    13141 gatctcatcg agaaacagag taccgttatc cgccatttca aacttcccgc tgcggttact
    13201 gatagcgcca gtaaacgctc ctttcacatg cccgaacaac tcactttccg ccacactttc
    13261 cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg aggcttcatg
    13321 aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctgatca gaacgttgag
    13381 atcggacgcc gccacaatct caatctcttt tttcagttgc gtcatgccag gggacaagcc
    13441 aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca gcatattctg
    13501 gctttccagt tgttcaatca gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat
    13561 cagccgtaac tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt
    13621 cagtgcgccg atcaggtttt gtccgacaaa caatggcaga ccaacgcagg cgtgaacctt
    13681 cagactctcc tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg
    13741 aaagcgcacc acatccccgg cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc
    13801 aaagcgtcta ccgagtacat cctttgccag accgtcgatg gcaagcggaa taaactgccg
    13861 cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgta gcgtggtgat
    13921 caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg cgatattcgc
    13981 cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat agatattgtc
    14041 atatcgacca tttgattgat agtcattttg actactcatt aatgagcata attttattta
    14101 tagagtaaaa acaatcagat aagaaactgg cacgcaatct gcaattagca agacatcttt
    14161 ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa taacattcat
    14221 tgggtgggtc aacgtgactg ggaagtgcgt gattttcacg gcacggaata taaaacgctg
    14281 cgcggcagca gctacaacag ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc
    14341 gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg
    14401 gatatcgatt acatcgtgat taaccatgca gaagaggacc acgccggggc gctgaccgaa
    14461 ctgatggcgc aaattcccga tacgccgatc tactgtactg ccaacgctat cgactcgata
    14521 aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg
    14581 gatatcggca acggcaaaca gctcattttt gtcgaaacac caatgctgca ctggccggac
    14641 agcatgatga cttacctgac aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag
    14701 cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct tttcgagcag
    14761 tgccagcgtt actacgccaa tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt
    14821 accgagatcc tgggctttaa cttaccagtc gatatgatag cgacctccca cggcgtggtc
    14881 tggcgcgata acccgacgca aattgtcgag ctgtacctga agtgggccgc tgattatcag
    14941 gaagacagaa tcaccatttt ctacgacacc atgtcgaata acacccgcat gatggctgac
    15001 gctatcgccc agggaattgc ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc
    15061 gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc
    15121 ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt ggaggagatg
    15181 accggattac gcttccgtaa caaacgcgcc agtgctttcg gatctcacgg ctggagcggc
    15241 ggtgcgatgg atcgcctttc cacgcgcctg caggatgcgg gtttcgaaat gtcgctcagc
    15301 ctgaaagcca aatggcgacc agaccaggac gctctggagt tatgccgtga acacggtcgc
    15361 gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt
    15421 aaagaagaaa cctctgccac gacgacggct gacctcggcc cacgaatgca gtgcagcgtc
    15481 tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg
    15541 ccgtggagtg aagtcccgga taacttcctc tgcccggaat gctccctcgg caaagacgtc
    15601 tttgacgaac tagcatcgga ggcaaaatga gcaacggcat tgtgatcatc ggttcgggct
    15661 tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgccactatt ccattaaccc
    15721 tgattgccgc cgacagcata gatgagtaca acaaacccga cctcagccat gttatcagtc
    15781 aggggcaacg tgccgacgac cttacccgcc agacggcggg tgaatttgcc gggcagttta
    15841 atctgtgcct gtttccgcac acctgggtga cggatatcga tgccgaagcc catgtggtga
    15901 aaagccagaa taatcagtgg caatacgaca agttagtgct ggcaaccggt gccagcgcct
    15961 ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt aaatagtcag caagagtatc
    16021 gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt gattgtcggc ggtggtttga
    16081 ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca
    16141 acgctgccag tattctggcg tcattaatgc caccagaagt aagcagccgc ttgcagcatc
    16201 ggttgacgga gatgggcgtt catctgctgt tgaaatctca gttgcagggg ctggaaaaaa
    16261 cggattctgg cattctggca acgctggacc accagcgtag tatcgaagtg gatgcggtaa
    16321 ttgccgccac cggactgcgc ccggaaacag ccctggcacg acgcgccggg ctgacgatta
    16381 atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt tacgcgctgg
    16441 gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct gcagccgatt caacttagcg
    16501 ggatggtgct ggcaaaaaat cttctcggca ataacacgcc gctgaaacta ccggcgatgc
    16561 tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg
    16621 atttacgctg gcagattaat accgaacgcc agggaatggt ggcgcgcggc gttgacgatg
    16681 ctgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggca tttgtattgt
    16741 tgaaaacgtt gccggtgtag gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg
    16801 cgtcttatcc gacctacggg gacgcatgtg taggccggat aaggcattta cgccgcatcc
    16861 ggcaagggtg tccaaatgca acacgcttta tccgttctgg acttcacccg ctaaccaacg
    16921 cgccgcagca ataaccccct gccccagaga caaaccgcca tcacccgccg gtaaactctg
    16981 tggaaagagc aatgtgaaat cagcgagata atgcgccaga cgtgcacgca gcaaacggtt
    17041 atgaataacc ccgccgctaa ataccagcgt agtgatacca cgcatcgtgg cctgctcacg
    17101 catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac gcccacgcgc gttgattaac
    17161 cagtgcctgc cagttcagcc actgctgcca gaaagtggcg agatccagtt gattgtccac
    17221 ccgcggcatc gtcaccggat gcgtcactcc gtcgcatgag gctgcgagcg cctccagagc
    17281 acaagccgct tcaccttcat aacttaacgt ggctggcgca cagcccagtg ccgccgccac
    17341 tgcatcgaac aaccgcccac acgatgatgc cagcggcgcg ttaattccac gctcaatggc
    17401 ccgcgccagt atgctccagt tttgctgttg cacacttgcc gtttcagggt aattctgcca
    17461 ctccggcaca aagcgcaggc actgcgccag caggtttcgc cacggctgct tcgccgccaa
    17521 atcgccaccc ggaagcgcca ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt
    17581 cacccgcagg cactcgccgc cccacaaagc gccattctcc cccataccga taccgtcgag
    17641 cgtcaaagca atgacatcac cgccatccag cggccactga tgctctgcca gacacgccgc
    17701 tgcatgggca tgatgatgca gtaccgtttg cgtcggcaga ttcatttcgc tcgcccactg
    17761 gctggagaca tagcccggat gcacatcatg cacaatgtat tgcggagtga aatcgtagat
    17821 gttttgcatc aggcgtaacg cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc
    17881 gcccagatgc tgactcaaca ccgcttgttc accgcgcacc aggcagaagg tatttttcag
    17941 atccgcgcca agacacagca caggcggaac atttttaaag cccggaggca aaaccagcgc
    18001 atccggcaca tacccccgcg aacggcgcag catttcgccg ctttcgcgca ccactgaatc
    18061 atccatccgc tgcacgatgt cgcggttatg tagcaagaat ccgtcggcaa tgccctgcaa
    18121 atccgccagc gcctgttcgt tgctgatagc tggtggttta ccgctcaggt tgccggaggt
    18181 catcaccagc gggcattgca gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa
    18241 catcaccccg acttcgttaa ggccaggggc gatatcatca caaagctcag gtacgtattt
    18301 tttatccacc agcacaatcg gcgcagcggg cgtggtaagc aactggcgcg cagcgtctgg
    18361 taaactttct gccactggca acatgactgc cagcggtttc gccgggcgat gtttgcgctc
    18421 ccgcagtgtc gccaccgcgt tactgttacg tgcatcgcag gcaagatgaa atccgccaat
    18481 ccctttgatg gcgacaatgt tgcccatttt taactgtgcg atagctgcct gtaatgccgc
    18541 ctcttgttct gcatgttcac catgacttac ccattcaaga tgcgggccac actccgggca
    18601 ggccaccggc tgggcgtgga agcgacgatc gagcgggtcg cggtactctt tgtcacaggc
    18661 cggacatagc ggaaacgccg ccatcacggt aaacgggcgg tcgtaaggca tcgcgcgaat
    18721 aatggtgaaa cgcgggccgc agtgggtaca gttgataaac ggataacgat aacgccgctc
    18781 gcctggggta ttcatttcgg caaggcaagc agggcaagtg gcggcatccg ggacaatttg
    18841 cgtattcatg gtgccgccgg tgctctggcg gatggtgaac tcggtgggca gttgtgacca
    18901 gataaacggc tcacgctcga cgctatcaat acgcgccagt ggcggacagt gctgatgcaa
    18961 ttgaacaaga aacgtttccg ggtcttccag cagccggact tctacgccat cgccgtcatt
    19021 acatacatcg ccgtgaagat ttaattgctg tgccagctgc cagacaaacg gacgaaaacc
    19081 gacgccctgc actttgccac gaatacgcag ttggacaccg caagatgtgt tttttgccat
    19141 tgagttattc ccgccatcat gaattgcgta acccgccctg ccggacacga cagcgtcgta
    19201 tccggcagtc tcaggtcggc gataccgccc gctccgtatt ctacgaatat ttccgggaat
    19261 tcctttgatg ccagaacagt tctgtaagat ttttagaaca tcagcgccgt acggcggcgt
    19321 ttttctgcgc tcagttgttc aagtttatta cgatcgacac aaatcagcgc atgggtcggg
    19381 caagccgcca tacacgccgg gccgtcttca cgatggttgc acaggtcgca tttattggct
    19441 tcggctttgt cagcccgtac attcagaccc gcgccgctgt tgcgaatcac aggacgtacc
    19501 accacttcca tcgcgccata cgggcaagcc accacgcagg ttttgcaacc aatgcaacgt
    19561 tcctgcatca caggaacaaa ccctttatca cagctgatag caccattcgg gcagacgtta
    19621 gcgcacggtg catcttcaca ctgacggcaa actgtcgccg tggaaatgtt cacaccttta
    19681 atgacatgga tacgcggtaa aaatgtttcc ggggtcagcg atgcacagtc ctgattttcc
    19741 tgatgagaaa ccacgcacgc cacttcacag gtacggcaac caatacattt actcgcgtca
    19801 gcaatgatga aacggttcat caaattctcc agcaatgaca gttaatgcgc cgatacactc
    19861 acaaatcatg ccagttttta atttgctgtt atttaaggaa attaatttct gtaatgcagg
    19921 aaaaaagatg tcatcgacac tagtgacgat gacatgtgat gacaatgttt atcgcgaaag
    19981 ggcaatgagt gagtcgcggc ggatcagttt tccgctaaag gttttcggcg gtgagaaatc
    20041 cccgccatcg agcataaaaa tcagccgtcc aataatttcc tgaatcatct cagtcaccgg
    20101 aatttttacg ctggagagcg ccggaacgat atagggggca attgcgatat catcgaatcc
    20161 gataactgac acctgctctg gcaccgctac gccgcgctcg tgcaacgctt tgatcgcacc
    20221 tatcgccata tcgtcgttac tggcaactaa cgcgctaaat ttagcccctc gttcgagcaa
    20281 cgtttctacc ccttcggccc cgctggcggg cgtccattta ccgttagcga taagtttttc
    20341 attgagcgca ataccatgct gcgacagcgc gtctttatac ccggcaagac gttcaatgct
    20401 ggtgggggaa tccatcgagc cggtaaggaa agcaatctcc tgatgcccgg cgtttatcaa
    20461 ctctgccacg gcgttaaagc tggtctgttt atgatcgcac cagacgctat ggctgctatt
    20521 attgcgcagg cggcgattaa gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc
    20581 aatctcatcc acgcttaaaa aacgcgggta aatcataatg gcgtcgcagc gcaaatccag
    20641 cagatactga atcgcctggc gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa
    20701 ctgtcgccct ttctcttccg ccattcgcgc ggcatgaaac agtaattcac taaaataaat
    20761 gccgtgataa agcgtgttgg tcactaccag ccccagcgtc tgagtactct tcgccgacag
    20821 attgcgggcc agcaaatttg ggcggtaact gctctcttct accgcctgaa acacgcgatc
    20881 tttagtctcc tggctgacgt agccattacc tgaaagcacg cgggaaacgg tcgcttttga
    20941 aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc attttcatcc ctttacacgc
    21001 aatcaacgca gtgtactgca ccgtttgccg attgtccttg cgcaatcggc tccagcggat
    21061 aagacgaggt cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc
    21121 cttatttcag cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca
    21181 ccactttgct cttcttctta cgcacatcga ctacggtcat gcggtcggta caggagtagc
    21241 aagggtccag gctaccgata atcagcggcg cataggaaac ggtattgcca cgcagcatgt
    21301 agcgcagggt cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct
    21361 ggttgtcgcc ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca
    21421 gcgcgaaacg gtgcggaatg taggtaaagc cttccaccat cagcggaccg cccggcaggt
    21481 tatccagacc gtagtcgatc atattcagcg cggtatagac ctcgttgatt cgcactttca
    21541 gacgggaaat aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac
    21601 cgacaaacgg gtgatcggcg cgagtatcgc gggcgagacc gctggcgcga accatcgggc
    21661 caacgttact gaaatcgcgg gcgatttccg ggtccagacg accaatgccg acggtgcgtt
    21721 gttccatgtt cggcgtactc agcagcacat ccaccagctc ctgcacttca cgacgcattt
    21781 gttgcgccag ctggcgggtc tggatcatgt catctttcag cagatcgcga cgaatcccgc
    21841 cgatcaggtt caggccgtag gttttacgcg caccggtaag gatctctgcc attttcatgg
    21901 aggtttcacg cacgcggaag aactgcataa agccggagtc gaagccggta aagtgacagg
    21961 cgaggccgag gttgagcaga tgcgggtgca ggcgttccac ctccagcaga atggcgcgga
    22021 tcatctgcgc gcgttctggc acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg
    22081 cggtgctgtg ggcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt
    22141 tataacccat gcgggtttcc gccagttttt ccataccgcg atggacgtag aacagacggt
    22201 agtcggcgtc gataatgttt tcgccatcga cgaacagacg gaagtgaccc ggttcgtcag
    22261 aagtgacgtg cagcggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga
    22321 tgaactcgta ggtttcagca tcggtagtcg gtgccggacg ctgacgataa tccatgctgt
    22381 ctttacgcag cggataaagt tcatccggcc agtcatccgg cagcaccaga cgacgttcat
    22441 ccggcagacc aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg
    22501 ccgccggaac gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc
    22561 gaaccgttac ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat
    22621 gaccattcag tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt
    22681 agtagagaaa ctccaccact tccggcagat agttcacctt tacggtgaca gtcagctgat
    22741 ctttggtctg ccaggcgtgg tccagcacga cgcctagaaa tgcctcattc agcgcggcga
    22801 gataatgttg acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag
    22861 caaggagacg aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat
    22921 atcaagacgc agacgggcca tgctgttttc gaacagcgcg ataaccagga cgccgaccac
    22981 cagtttgacg atggcaatca ccagcgccag cagcagtcca ccgacggtga aggtttccat
    23041 ttgtccccac ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct
    23101 gataccccat ttcatgacgc caaagccgct accgctgtat tcggagagcg gaccttcctg
    23161 caactcctgc tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc
    23221 gaacgcacag gcgcagagcg ccagcaccag cgggatgctt tggctcagcg gccagtgata
    23281 aacggtgtcg gtaatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag
    23341 accaagcagc aacatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcaccgat
    23401 agcggtaaac gggctaccgg tatccagacc ggaaatggca aagaagaaac gcgcgatggc
    23461 gaagagatac agtaaggtga tcaaatcgcc cagttgcggc agcggagaac cgacggtcac
    23521 caccggcagc gcagtagcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg
    23581 gaacatccag ccagaggcat ccgggccaac gctctggcgg cccagcagtt tgataatgtc
    23641 gcgatactcc tgcaacacgc ccggcccgcg acggttatgc agacgggcgc gcgccacgcg
    23701 ggtaataccg gagagcagcg gcgcaacggc aaataacacc agcgcctgaa ttaacggata
    23761 taaaacactc attctcaggc tcctcgtgaa acaataatca ccaccagtac cgccagttca
    23821 accagcgcca tccggcggaa cagcaacgca ctaccttcgc actgccagcc cggcactagc
    23881 gacaccggat tcagccattt gcgcagtttc agcactggcg caaacgcctg tttaaccggc
    23941 atggcaaaac cgtgagcggt aatcaccatt gatttttcat ggtcgtagcc gcacacccag
    24001 gccgcaccgc gggaacgtga cggcaggcgg tcgcctttgc aaatcgccat aatgatgaat
    24061 ggcagcagcg ggcaggcaat cagcagcaat gtgatcattg gttgagaaac ggtggtgtta
    24121 gcaggctcca gaggcagagg tacagcagca gagagcatcg gcagcagcca cggcgcagca
    24181 acaccgccaa ttacgcagca aatcgccagt gccactacgc ttacgctcat caggagcggc
    24241 gcacaggtgg cgttttcggc ttctttggta cgcggcgcgc cgaggaacgt gacgccatag
    24301 actttcgcca tacacatcac cgccagcgca ccggtaattg ccagacccac agcgagcagc
    24361 ggccccagca gacgggcaac aaacgcgccg ctattgctca gtttgaagaa ggattgatag
    24421 ataacccatt ccccggcaaa cccgttcagt ggcggcagcg cagccattgc catcaacccg
    24481 actaacatgg cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata
    24541 tcgcgatgac cggtacggaa ccagacgctc cccgccccga ggaacagtac gcttttgaac
    24601 aggctatggt taagcagatg gtacagacca ccgaccaggc caagagcaat cagcgccggt
    24661 tgttcgagcg cgatacc
//
LOCUS       DBBBSB010000059        24657 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.059, whole genome shotgun sequence.
ACCESSION   DBBBSB010000059 DBBBSB010000000
VERSION     DBBBSB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24657
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.059"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..386)
                     /locus_tag="ACIUS0_002309"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACIUS0_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACIUS0_002310"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACIUS0_002310"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGB0220035.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACIUS0_002311"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACIUS0_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGB0220036.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACIUS0_002312"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACIUS0_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGB0220037.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACIUS0_002313"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACIUS0_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGB0220038.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACIUS0_002314"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACIUS0_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGB0220039.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACIUS0_002315"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACIUS0_002315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGB0220040.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACIUS0_002316"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACIUS0_002316"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGB0220041.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACIUS0_002317"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACIUS0_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGB0220042.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACIUS0_002318"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACIUS0_002318"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGB0220043.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACIUS0_002319"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACIUS0_002319"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGB0220044.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACIUS0_002320"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACIUS0_002320"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGB0220045.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACIUS0_002321"
     CDS             10748..11212
                     /locus_tag="ACIUS0_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGB0220046.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACIUS0_002322"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACIUS0_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGB0220047.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACIUS0_002323"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACIUS0_002323"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGB0220048.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACIUS0_002324"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACIUS0_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGB0220049.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACIUS0_002325"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACIUS0_002325"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGB0220050.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACIUS0_002326"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACIUS0_002326"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGB0220051.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACIUS0_002327"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACIUS0_002327"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGB0220052.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACIUS0_002328"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACIUS0_002328"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGB0220053.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACIUS0_002329"
     CDS             complement(20505..20798)
                     /locus_tag="ACIUS0_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGB0220054.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACIUS0_002330"
     CDS             complement(20795..22084)
                     /locus_tag="ACIUS0_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGB0220055.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACIUS0_002331"
     CDS             complement(22140..23078)
                     /locus_tag="ACIUS0_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGB0220056.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACIUS0_002332"
     CDS             complement(23098..23862)
                     /locus_tag="ACIUS0_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGB0220057.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24657
                     /locus_tag="ACIUS0_002333"
     CDS             24181..>24657
                     /locus_tag="ACIUS0_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGB0220058.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLAI"
BASE COUNT         6105 a         5950 c         6485 g         6117 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataacg gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agcaatt
//
LOCUS       DBBBSB010000060        24647 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.060, whole genome shotgun sequence.
ACCESSION   DBBBSB010000060 DBBBSB010000000
VERSION     DBBBSB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24647
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.060"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..246
                     /locus_tag="ACIUS0_002334"
     CDS             <1..246
                     /locus_tag="ACIUS0_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGB0220059.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACIUS0_002335"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACIUS0_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGB0220060.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACIUS0_002336"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACIUS0_002336"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGB0220061.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACIUS0_002337"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACIUS0_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGB0220062.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACIUS0_002338"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACIUS0_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGB0220063.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACIUS0_002339"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACIUS0_002339"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGB0220064.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACIUS0_002340"
     CDS             complement(4442..5650)
                     /locus_tag="ACIUS0_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220065.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACIUS0_002341"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACIUS0_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGB0220066.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACIUS0_002342"
     CDS             complement(6445..8130)
                     /locus_tag="ACIUS0_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGB0220067.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACIUS0_002343"
     CDS             8400..8777
                     /locus_tag="ACIUS0_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGB0220068.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACIUS0_002344"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACIUS0_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGB0220069.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACIUS0_002345"
     CDS             9224..9511
                     /locus_tag="ACIUS0_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGB0220070.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACIUS0_002346"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACIUS0_002346"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGB0220071.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACIUS0_002347"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACIUS0_002347"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGB0220072.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACIUS0_002348"
     CDS             11268..11744
                     /locus_tag="ACIUS0_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGB0220073.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACIUS0_002349"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACIUS0_002349"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGB0220074.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14436
                     /gene="potG"
                     /locus_tag="ACIUS0_002350"
     CDS             13303..14436
                     /gene="potG"
                     /locus_tag="ACIUS0_002350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGB0220075.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14446..15399
                     /gene="potH"
                     /locus_tag="ACIUS0_002351"
     CDS             14446..15399
                     /gene="potH"
                     /locus_tag="ACIUS0_002351"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGB0220076.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15396..16241
                     /gene="potI"
                     /locus_tag="ACIUS0_002352"
     CDS             15396..16241
                     /gene="potI"
                     /locus_tag="ACIUS0_002352"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGB0220077.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16301..16789
                     /locus_tag="ACIUS0_002353"
     CDS             16301..16789
                     /locus_tag="ACIUS0_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGB0220078.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16830..17957
                     /gene="rlmC"
                     /locus_tag="ACIUS0_002354"
     CDS             16830..17957
                     /gene="rlmC"
                     /locus_tag="ACIUS0_002354"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGB0220079.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACIUS0_002355"
     CDS             complement(18132..18863)
                     /gene="artJ"
                     /locus_tag="ACIUS0_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGB0220080.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACIUS0_002356"
     CDS             complement(19155..19823)
                     /gene="artM"
                     /locus_tag="ACIUS0_002356"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGB0220081.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACIUS0_002357"
     CDS             complement(19823..20539)
                     /gene="artQ"
                     /locus_tag="ACIUS0_002357"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGB0220082.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACIUS0_002358"
     CDS             complement(20546..21277)
                     /gene="artI"
                     /locus_tag="ACIUS0_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGB0220083.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACIUS0_002359"
     CDS             complement(21295..22023)
                     /gene="artP"
                     /locus_tag="ACIUS0_002359"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGB0220084.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22241..22756)
                     /locus_tag="ACIUS0_002360"
     CDS             complement(22241..22756)
                     /locus_tag="ACIUS0_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGB0220085.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23405..>24647
                     /locus_tag="ACIUS0_002361"
     CDS             23405..>24647
                     /locus_tag="ACIUS0_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGB0220086.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISF"
BASE COUNT         6196 a         5935 c         6266 g         6250 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctgcg tatgctggca
    13501 ggtttcgaac aaccttctgc cggacagata atgcttgatg gcgtcgattt gtcacaggtt
    13561 ccgccttacc tgcgccccat caatatgatg tttcagtctt acgcgctgtt tccgcatatg
    13621 accgtggaac agaacatcgc ttttggcctg aaacaggaca aactaccgaa agcggaaatt
    13681 tccagtcggg tcaatgagat gctcggcctg gtgcacatgc aggagttcgc caaacgtaaa
    13741 ccgcatcagc tttctggtgg tcagcgacag cgtgtggccc tggcccgaag ccttgccaag
    13801 cgcccgaaac tattactgct cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac
    13861 cggatgcagc ttgaagtggt ggatattctg gagcgcgtcg gtgtgacttg tgtgatggtc
    13921 acccacgatc aggaagaggc gatgaccatg gcggggcgca tcgccattat gaatcgtggg
    13981 aaatttgtcc agattggcga accggaagag atctacgagc atccgactac ccgctatagc
    14041 gccgaattta ttggctcggt aaacgtcttt gaaggcgtac tcaaagagcg tcaggaagat
    14101 ggcttggtgc ttgattcgcc ggggctggtg catccactga aagtcgacgc ggatgcctcg
    14161 gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa
    14221 gagccgcccg ccaatggttg taacttcgcg gtgggggagg tgatacacat tgcctatctc
    14281 ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc agatgattag tgcccagcta
    14341 caaaacgccc atcgtcatcg taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc
    14401 tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag caaagatgaa tacacttgaa
    14461 cctgctgccc agtcgaaacc gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg
    14521 aagcatgggc gcaaactggt cattgcgttg ccatatatct ggttgatctt gctgtttctg
    14581 ctgccatttc tgattgtctt taaaataagc ctggcggaga tggcgcgcgc tattccacct
    14641 tataccgaac tgatggagtg ggctgacggg caactgtcca tcactcttaa tctcggtaat
    14701 tttctgcaac tgaccgacga tccgctctat ttcgatgctt atctccagtc gctacaggtg
    14761 gctgcgattt cgacattttg ctgtttactg atcggctatc cgctggcgtg ggcggtggcg
    14821 cacagtaagc cttcgacccg taatatttta ttactactgg tgatcctgcc gtcgtggacc
    14881 tcgtttctga tccgcgttta tgcttggatg ggaatattaa aaaacaacgg tgtgctgaat
    14941 aattttctgc tgtggctggg ggttatcgat caaccgctga ccattcttca taccaatctg
    15001 gcagtttata ttggcattgt ttacgcctac gtgccgttta tggtgctgcc gatttatacc
    15061 gcgttgattc gtattgatta ttcgctggtg gaagcagcgc tggatctcgg tgcacgaccg
    15121 ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag gtgggattat tgccggatcg
    15181 atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc cggaactgct cggtggcccg
    15241 gacagcatca tgatcgggcg cgtgctatgg caagagtttt tcaataaccg cgactggccg
    15301 gtggcctcgg cggtagcgat catcatgttg ctgctgctaa ttgtgccgat aatgtggttc
    15361 cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa taatttaccg gtagttcgtt
    15421 cgccctggcg gattgtgatt ttgctgctgg gctttacctt tctctacgcg ccaatgctga
    15481 tgctggttat ctattcgttt aacagctcga agctggtgac ggtgtggggc ggctggtcaa
    15541 cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat gagtgcggtt ggttcaagcc
    15601 tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct cgggactatt gcggcggtgg
    15661 tgctggtgcg ctttggcagg tttcgcggat caaatggctt tgcctttatg atcaccgcgc
    15721 cgctagtaat gccagatgtc atcacgggct tgtcgctgtt gttattattc gtcgcgcttg
    15781 ctcatgccat tggctggcct gcggaccgcg gtatgcttac catctggctg gcgcatgtca
    15841 ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct
    15901 cgatagaaga agccgcgatg gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta
    15961 cgctaccgat gatcatgccc gcgatcattt ctggctggtt actggctttt actttgtcgc
    16021 ttgatgatct ggtgatcgcc agctttgttt ccgggccggg agccaccacg ttaccgatgc
    16081 tggtcttttc cagcgtgcgg atgggggtga atccggaaat caacgccctg gcaacattga
    16141 tcctcggcgc ggtcggaatt gtcggattta tcgcctggta tctgatggcg cgcgcagaaa
    16201 aacagcggat acgcgacatc caacgtgcaa gacgtggctg aagacactaa aatttgccaa
    16261 cctggctaca taatgccgcg catgtcgcgg cattgttttc atggaagacg aaacgttggg
    16321 attttttaag aaaacatctt catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt
    16381 ggcggcgctc gccattatta tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct
    16441 gggcgtgcgc ggtattggcg agttcattca tcgcagcgta caaacctgga gtttaacgct
    16501 ggtcttttta agcagtctgg tgctggtttt cattgagatc tggtgtgcgt tttcactggt
    16561 gaaagggcgt cgctgggcgc gctggctata tctgctgaca caaatcaccg ccgcaagtta
    16621 cttgtgggcg gcttcgctgg ggtacggtta tccggagctg ttcagcattc ccggtgaatc
    16681 aaaacgtgaa atcttccata gcctgatgct gcagaagctg ccggatatgc tcatcctgat
    16741 gctgctgttc gttccctcga ccagtcggcg gttcttccag ttgcaataat gtgtataatc
    16801 gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct
    16861 gtcgttcctg tcagtggata acgcagccga ttccagagca actctccgct aaaaccgccg
    16921 atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc
    16981 cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag tggtagcgtt gaaaaaacac
    17041 tgctcggtat gctgcatcgc gatggcacgc cggaagacct ttgtgactgc ccgctttatc
    17101 cagcctcatt tgcgcccgtt tttgcggcgc taaaaccgtt tatcgcccga gcggggttaa
    17161 cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata cattctgctg actgaaagcc
    17221 agagtgatgg cggcatgatg ctgcgctttg tactgcgttc tgataccaag ctggcgcaac
    17281 tgcgtaaggc gctgccgtgg ttacacgaac aactaccgca gctgaaagtt attaccgtca
    17341 atattcagcc ggtacatatg gcgattatgg aaggggagac ggagatctac ctgaccgaac
    17401 aacaggcact ggcggagcgt tttaatgacg taccgctgtg gatccgtccg caaagtttct
    17461 tccagactaa tccggcggtc gccagccagt tgtacgccac cgcgcgcgac tgggtacgac
    17521 agctgccggt taaacatatg tgggatctgt tctgcggtgt ggggggcttt ggtttacact
    17581 gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc gccagaggcc attgcctgtg
    17641 caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca atttcaggcg ctggactcca
    17701 ctcagtttgc cactgctcag ggggaggtgc cggagctggt gctggttaac ccgccgcgcc
    17761 gcggcattgg taaaccgctg tgtgattatc tttcaacgat ggcaccgcgt tttattatct
    17821 actccagctg taacgcccaa accatggcga aagatatccg cgaactgcca ggttaccgca
    17881 tcgaacgggt acagcttttt gatatgttcc cgcacaccgc gcactatgaa gtgttgacgc
    17941 tgctggtgaa gcaataaaaa agccacaggt gcggcttcag attgctgatc aggtgcgctt
    18001 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaatcatg ctaaatcaat
    18061 acattgcaga gttgatgtgg gccggataag cgcagcgcat caggcaatct tacttttgtc
    18121 atcagtctca attactgcgg aaaccactgg tcattgattt tctgatatgt accgtcagct
    18181 ttaattgctg ccagcgcgtt attcagtttt tccagcagag ctttgttatc cggacgtaca
    18241 gcgataccca ggccggtgcc gaagtattgc ggatcggtca ctttttcagt cgcaacgccc
    18301 agttgcggat tggttttcag ccattcgttt accaccgcgg tgtcaccaaa taccccatca
    18361 atacgaccat ttttcagatc gataaaggca ttctgataac tgtcatagga gacagttttc
    18421 acttccgggt gctgatcctg aatatatttc tggtgcgtcg taccgttttc catcccaatg
    18481 cgtttgcctt tcaggtcggc aaacgttttg taggtatctt ttttggaaat cacgacggct
    18541 gagttttcat agtagggtgt ggtaaacgaa acctgcttgc tacgctccgg ggtgatatcc
    18601 ataccggaga ttacggcgtc atattttctg aatttcagtg acgggatcag gctgtcgaac
    18661 gcgtgattag taaaagtaca ttctgcctgc atttgtttgc acaaggcttt tgccagatcg
    18721 atatcaaagc cgacaatctc attattagca cctatagatt caaagggtgg ataggtggct
    18781 gaaacgccaa aattgatttt ctctgcggca gaagcaccga aagtaaagga agcaagtaaa
    18841 gcggcaagaa ctaacttttt catgatggaa ctcccgtctg tcaatcttat gatttttggc
    18901 cgtgtctgcg gcatgggata acaatgccat caagtgaatt tatatgcaat aaacatgatt
    18961 aaataattta aatgaaataa aaaaagacgg ataacttagt gagttgtccg tcttcattat
    19021 aagaatttat gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc
    19081 gtgctgatgt ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa
    19141 tgtacgcagc tacattaatt ccgccgttcg aatgccagtg ctttgcgctc gatcagacgc
    19201 atcatcagcg tcagcaggcc gttgacgacc aagtaaataa tccctgccgc accgaacacc
    19261 attacatcgt aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta
    19321 atggtgtaag ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag
    19381 agcgagcgtt taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc
    19441 agggcgctac aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc
    19501 tgcgtggtat acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct
    19561 gacaacaaat gccacagtgc cggatactcc tgcaacgtcg gaaactggcc cggcccgtaa
    19621 taaatcagga agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc
    19681 accagccaca ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga
    19741 atcagtgcca caatcagcga ggcaacggtt agcgtcaggc tggtgtgcag ccctttcatc
    19801 agctcgggta aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt
    19861 caatgcgttt gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca
    19921 caatgtacca ggtaaatggt tcctgggtac gagtagcaat gcttttggtt tgtaacatta
    19981 aatcattcac actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt
    20041 taccgaggcc aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag
    20101 ccgatttcga cagccccagc gcctgaccag attcccactg gcccaccggt acagctttca
    20161 acgcgccgcg cagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac
    20221 cacaaaggaa cggactcacg tcgaagttct caatgtccat ctgcaccggg atctgcacga
    20281 acccaagatt gatagtgaag ccatccgaaa gcgtcagcag taactgcgag gagccaaaat
    20341 agataaacag cactaccaga atttctggca ggccacgcag aatggttacc agcgctgaac
    20401 ctgcccacgc gacaggacgc catttcgctg actcccatac cgcaaagaac atcgccagcg
    20461 ccagcccgac aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta
    20521 aaggaaaaaa ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt
    20581 aagtgccatc tttcttcact ttttccagcg cagtgttgag tttctgctgc agttcagtgt
    20641 ttccctgacg taccgcgatg ccgaggccag taccgaaata atctttatcg gtcactttat
    20701 cgcccactgc cgccagtttt gggttatctt tcagccactc agtgaccact gcggtgtcac
    20761 caaatacgct gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat
    20821 acggaacggt ggtgatttcc gggtgcttat ccataatgaa cttctggtgt gtcgtcccgt
    20881 tctgaacgcc gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac
    20941 ccacaaacag ggcagagttg tcatagtacg gggtagtaaa cagtacctgc ttttcacgct
    21001 ccggagtgat atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga
    21061 tcaggctgtc aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg
    21121 cctgcgccag gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg
    21181 gaggataggg ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa
    21241 aacctgcaat taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg
    21301 agagagatag tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg
    21361 ttctacgata tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc
    21421 aacttcgtgg gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct
    21481 gacgatttgt gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac
    21541 ctgcggttcc atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag
    21601 atgcagcggg taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc
    21661 acgggccagc gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt
    21721 ttgctgcacg gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg
    21781 acgcaaatcg cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc
    21841 aatgttgagc gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct
    21901 tttacccgcg ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt
    21961 gatatcgaac agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact
    22021 cattgacact cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttaac
    22081 agaataatta tgcaatattt atgctttaaa agttaaaagc aaagcgcatt attcaataaa
    22141 catagcacaa aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa
    22201 atgccggata atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact
    22261 gacggagcgt acccgccgtg gcatgaacgc taccgcctaa gtagcgcaca tcgtcgatga
    22321 cccaacactg gccttcctga atcattaaca cttcatcctg ccagccctgg tcaccctgtt
    22381 tgagatccac ccgcaatgga atgttacggg catcacgatt agggatagtc gatgcactgg
    22441 caacgtgggc gctgtctggc aaggtggttc gactggagaa cggatcgctg cttaacaatt
    22501 cacgatgact attatcgcgg ctggcatcgc taagttgggt tgccagtttg tcactcaaat
    22561 aggggcgcag ggcggtaatg tcattgctgc gatgttgaat gcggtagtcg tagaattgtt
    22621 gagccacact gtcaggccct ccctggacac aggaaccggt gcgggggccg ttatctttat
    22681 aagctggcgt gacggtggta caggcactca gcagcagtgc acatggaata agtagagata
    22741 gtgaacggta gcgcataatc atttccttat tagcttgctg tctgcgtcac actacgacca
    22801 aacgctatca gattatttaa tacattgttt ttaatacaat tctgaaacaa tctgaacacc
    22861 tgattgggtt tgagtccatt gctggcgacc cactcaaggt tgctcgtagc tgacttactt
    22921 aaattaccca aacaatagac agtgtaaacc atttgtgagg ttgagcgtgt tatatggctg
    22981 aaatgatcat ttaaggtaaa tatgatagtt ctgaaatggt ggtataaatc ctcctgtaga
    23041 ggttaagagc atctgcaaga agccagtttc acatttataa cgcgtgataa atgaatgcgg
    23101 ttattgtcta atcattacaa aatttttcac atgcgataag agatcctgcg attaagatga
    23161 ttgccaaaat tacgttgtat ttttgaaaat gagcgaaatt tgatagtccc agctttgact
    23221 ctcgataata ttatttatgc tacaattgtg ggtaaagaat ttaagctaat gaatttgagt
    23281 gaagaataaa ttaaattgct tcagtatttc gttaattaat ataaatacaa aaggcgttgt
    23341 gcttttataa ttataaagcg cgaggagttc ggtggattat cgtttttctt gggtgatttt
    23401 taatatgata aacaatatat ctgatcaagc atcatcattt ccaggcacac aactaaatca
    23461 aagtgataat ttcctggatt cactgaggga atttttcgcg atattaaatc ccagtcgtaa
    23521 gggtgagttg tctacatggg ataccatcta cttgcatctg atattagcaa taaatgctga
    23581 cagtgatctc ataaaaaatg atgtgttgct cgcggaaaat attccatcag cgaattacca
    23641 atttaataca ttttttagca atactttcga gattgatgtt aaaaaatatc tgggtaaatc
    23701 agaagataac gaagttgaaa taaaagcggg taatgaaaga atctctattg gaatacgtaa
    23761 tgtatcgaat ggaaaacttg aaagacaaca gtttcttttc ccactggact atgagaataa
    23821 attacaagaa caattagata agtattttac gattgaatcg cacccattat tatacaggta
    23881 taccattggt agtaagattg cgaatgtgat ttttgaaaag ttatacagtc gtattgactt
    23941 taacaaagaa caatatatat cctttataaa ggacgcattt attcattttt atgattatag
    24001 caggcgatat gctattagtg agaatatcga taaagacgct gtaacaaaca atatcgcatt
    24061 gatgagcact ttttatgaca gtgataatac gtcaggtgag gttttaaata atgattttac
    24121 agaagaagag tcatttgaaa ctgcgttaga tgtagagcat gcaatagtgc ttggatttgc
    24181 tgatgataat tttgaaacaa aacctgttca ttatcaggat ttattaacac gatttagtgc
    24241 ctttcaagat accgtgttta atctgtttcc tgaaatgcac tcttcgcatt accacgatat
    24301 ttgtagcgta agtgtggata tgacaaaggg aacgcaatgc atgattcact tgatggtgaa
    24361 tgaagaagtt ttcatgtctc tgcctgttcc agttgccacg atggtacgtg aagatgcaag
    24421 taatttagtc aatcttaaaa cactactcaa tgatggttgt ttcattaaat atagtcattt
    24481 taatgacgtg gcattaataa agcaaaacat aagtaatctc tatttatcac acactgttgt
    24541 gaatgaaagc atattgaaaa aatgttgttt tgaaaacggc agtttaggtg atgttaaaat
    24601 aacaaatagc aatgtaataa acagtgcatt caaaaatatt tcgttta
//
LOCUS       DBBBSB010000061        24184 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.061, whole genome shotgun sequence.
ACCESSION   DBBBSB010000061 DBBBSB010000000
VERSION     DBBBSB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24184
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.061"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(25..597)
                     /locus_tag="ACIUS0_002362"
     CDS             complement(25..597)
                     /locus_tag="ACIUS0_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGB0220087.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="ACIUS0_002363"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="ACIUS0_002363"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGB0220088.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACIUS0_002364"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="ACIUS0_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGB0220089.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACIUS0_002365"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="ACIUS0_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGB0220090.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="ACIUS0_002366"
     CDS             complement(3559..5340)
                     /locus_tag="ACIUS0_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGB0220091.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="ACIUS0_002367"
     CDS             complement(5333..7105)
                     /locus_tag="ACIUS0_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGB0220092.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACIUS0_002368"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="ACIUS0_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGB0220093.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACIUS0_002369"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="ACIUS0_002369"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGB0220094.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="ACIUS0_002370"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="ACIUS0_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="ACIUS0_002371"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="ACIUS0_002371"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGB0220095.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACIUS0_002372"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="ACIUS0_002372"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGB0220096.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="ACIUS0_002373"
     CDS             complement(11661..12032)
                     /locus_tag="ACIUS0_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGB0220097.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACIUS0_002374"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="ACIUS0_002374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGB0220098.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACIUS0_002375"
     CDS             complement(14246..14536)
                     /gene="hupB"
                     /locus_tag="ACIUS0_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGB0220099.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACIUS0_002376"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="ACIUS0_002376"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGB0220100.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACIUS0_002377"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="ACIUS0_002377"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGB0220101.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACIUS0_002378"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="ACIUS0_002378"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGB0220102.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACIUS0_002379"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="ACIUS0_002379"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGB0220103.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACIUS0_002380"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="ACIUS0_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGB0220104.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="ACIUS0_002381"
     CDS             21803..22381
                     /locus_tag="ACIUS0_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGB0220105.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="ACIUS0_002382"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="ACIUS0_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGB0220106.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6665 c         5828 g         6024 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctcag gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gtta
//
LOCUS       DBBBSB010000062        24089 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.062, whole genome shotgun sequence.
ACCESSION   DBBBSB010000062 DBBBSB010000000
VERSION     DBBBSB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24089
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.062"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACIUS0_002383"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACIUS0_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGB0220107.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACIUS0_002384"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACIUS0_002384"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGB0220108.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACIUS0_002385"
     CDS             1531..2478
                     /locus_tag="ACIUS0_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGB0220109.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESHYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACIUS0_002386"
     CDS             2548..3354
                     /locus_tag="ACIUS0_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0220110.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACIUS0_002387"
     CDS             3364..3771
                     /locus_tag="ACIUS0_002387"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGB0220111.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACIUS0_002388"
     CDS             3771..4265
                     /locus_tag="ACIUS0_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGB0220112.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACIUS0_002389"
     CDS             4331..5128
                     /locus_tag="ACIUS0_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGB0220113.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACIUS0_002390"
     CDS             5139..5927
                     /locus_tag="ACIUS0_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HGB0220114.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACIUS0_002391"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACIUS0_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGB0220115.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACIUS0_002392"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACIUS0_002392"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGB0220116.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACIUS0_002393"
     CDS             complement(8370..10001)
                     /locus_tag="ACIUS0_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGB0220117.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACIUS0_002394"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACIUS0_002394"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGB0220118.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACIUS0_002395"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACIUS0_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGB0220119.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15245..17429
                     /gene="espL4"
                     /locus_tag="ACIUS0_002396"
                     /pseudo
     CDS             15245..17429
                     /gene="espL4"
                     /locus_tag="ACIUS0_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACIUS0_002397"
                     /pseudo
     CDS             complement(17398..19135)
                     /gene="aceK"
                     /locus_tag="ACIUS0_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACIUS0_002398"
     CDS             complement(19319..20623)
                     /gene="aceA"
                     /locus_tag="ACIUS0_002398"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGB0220120.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACIUS0_002399"
                     /pseudo
     CDS             complement(20653..22254)
                     /gene="aceB"
                     /locus_tag="ACIUS0_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACIUS0_002400"
     CDS             complement(22523..23452)
                     /gene="metA"
                     /locus_tag="ACIUS0_002400"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGB0220121.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23609..24052
                     /locus_tag="ACIUS0_002401"
     CDS             23609..24052
                     /locus_tag="ACIUS0_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGB0220122.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5946 a         6145 c         6107 g         5891 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccact atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtga gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagattgagc gatttatgat atttttttag ttgtaatcat
    15181 attaaatttt ttcactgttc tgatagttaa aattcaagac atcaataaac aatgagatat
    15241 ttaaatgatt actcgtattc ctcgtagttc tttctctgca aatattaata atacagccca
    15301 gacaaatgaa caccaaaccc tgagtgaatt gttttataaa gaactcgagg ataaattttc
    15361 tggcaaggag ctggcgacgc ctctattaaa aagcttctca gagaactgta gacaaaatgt
    15421 tcgtcatatc tttagcaaca aggattttgt cattaaattt tccacgtccg tcttacaagc
    15481 tgataagaaa gaaattacga tgattaataa aaacgaaaac acgacactca ctcaaaccat
    15541 tgctacaata tttgaagaat acctaatgga aattttacct caacgctcag acactcttga
    15601 taaacaagaa ttaaaccaaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact
    15661 taatggtcaa tgttatttcc cggggcgccc caaaaccgta tagtataccg acacattgct
    15721 gcacaatata ttaatgatat ttatcagaat gttgattaca aaccccatca agatgattac
    15781 tcttcagctg aaaaatttct cactcacttc aacaaaaaat gcaaaaacca gactttggcg
    15841 ttgatttcca gccgtcctga ggggcgttgc gttgctgcct gcggtgattt cgggctagtt
    15901 atgaaagcat attttgacaa gatggaatca aatggcatca gtgttatggc agccatatta
    15961 ctggtggata accatgcttt gacggtccgg ctaagaataa agaacacaac tgaaggatgt
    16021 acccattacg tggtttcggt ttatgatcct aatgtaacta acgataaaat aagaattatg
    16081 agcgaaagca aagaggatat taaacactat tctctgatgg attttatgaa tgtagattat
    16141 agcctcctga aatggtcaaa tgatcatgtt attaaccaat ctgttgcaat aattccagca
    16201 cttccgaaag aacagctatt gatgttaaaa ggaactgtgg atgaaataac ccctccatta
    16261 tcaccagcaa cgatgaattt gctaatggca attggtcaga atcaccaact tacgcaactg
    16321 atgattcagc tccagaaaat gccagaacta catagaacag aaatgttgac tgcctataat
    16381 agtattaacc tccccggttt atatttggct ataaattatg gcaatgcgga tatcgttgag
    16441 actattttca attcattgtc agaaacagga tatgaagggt tactctcgaa aaaaaatctc
    16501 atgcatattc tggaggcaaa agataaaaat ggtttttctg gattattttt agcgatatca
    16561 cgtaaggata aaaatgttgt aacctcgatt ctgaacgtct tacctaaact ggccgcaaca
    16621 catcatttag ataatgaaca ggtgtataaa ttcctgagtg ccaaaaatag aacgtccagc
    16681 catgttttat accatgttat ggcgaatggt gatgccgaca tgctgaaaat ttttttggtc
    16741 gcgttacctt tgttaattcg cacatgtcat ttgactaaag aacaggtact cgatctcctg
    16801 aaggcaaagg atttttatgg ttgcccaaga ctatacctgg cgatgcaaaa tggacatagc
    16861 gatatcgtga aagttattct cgaagcattg ccctgtctgg cccaggaaat taacatttca
    16921 gcttccgata ttgtagatct tctgaccgct aaaagtcttg cgcgcgacac gggtttgttt
    16981 atggccatgc agcgcggaca tatgaacgtt attaatacta tttttaacgc attacccact
    17041 ctttttaata cgtttaaatt cgataaaaaa atatgaagcc cctcctcctg gcaaataatt
    17101 ctaatgaata tcccggtttg ttttcagcga tacagcataa acaacaaaac gttgtagaga
    17161 cggtttatct tgctttatct gaccatgcac gcctgtttgg atttaccgct gaagatatta
    17221 tggatttttg gcaacacaaa gcgccacaaa aatactctgc ctttgagttg gcttttgaat
    17281 ttggtcaccg ggttattgct gaattaatcc ttaatacatt aaataagatg gctgaaagct
    17341 ttggctttac ggataaccct cgatacattg cggagaaaaa ttatatggaa gctttactca
    17401 aaaaagcatc tccccatacc gtacgctaaa tctttgcctg cgccgatacg cataaacatc
    17461 ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag
    17521 gtcggcgtgc atctcttcaa acagcggacc aatacgcggg tcggcgcata gccagtggcg
    17581 aaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc tggcaagttc
    17641 gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc acttccgtca tgtagcaaat
    17701 ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg acaccgaagt ttttaaacag
    17761 catgtcgcca gggaaaatgt tagcagcggc aagctggcga atagcgttac cgtattcttc
    17821 aatggcgtcg cgcaactgct gaccttccac ttgttccagc cagatattga gcggcaccat
    17881 ccgccgctcg attatagaga tggcgaatta caatctgctc accaagatcg gtgatttttt
    17941 ccgctgcttc ctggagcagt aattccatta ataccgggga aatatgccgc ttctccagca
    18001 caaagttttc aaactcctgg gtgtccgcca ttcggcccac acggtcgtgc tctttcacca
    18061 gttgatagca ggcacgaacg tgagcggcag acatctcttt ctgcggcgcg aacttgtctt
    18121 tgatgacttt gaatacccga tcaaagcccg gcagcgtaaa caccaacatc accattccac
    18181 gaatacccgg cgcttcaatg aactgctcat tacagccctg tagataaacg agatattcgc
    18241 ggtagctttc ggttttggcg tgcttctggc agccgatagc catatacaat tcagcagtgg
    18301 ttttacctgg cagaatttcc cgtagccact cgaccagcgc tgcgggcagc ggtgcataaa
    18361 ccataaaata agaacgcgca aagccaaaaa caatgctcgc ttcggcggtc gtcgtcaggc
    18421 aggtatcaat aaataactcg ccgtcgtccg tctggtggat cggcagtaag aagggcaacg
    18481 tgccggaagg cgtgatcagt ttgcccacca gccaggcggc tttattgcgg taaaacagtt
    18541 cgttcgccac ctgtaaatga ctttccgcga ggttgtctgt ccccagcgtt tccgtcagat
    18601 ggcgaatgat gtaatgaatg tcacggcttt tattctgcca gcgcaggcgc agcggcaggt
    18661 cgctgataac gcgcatcagt agagattccc agccgtgatc ggggtgaaag tctttcgcca
    18721 gcgggcgggg aatggtacga aagcggcgct ctggctgaga gctaaagata aaaagccgct
    18781 cgggagtaag cgagcggtgg tcaaataacc gacagtacac ggagttaaaa aagctctccg
    18841 caatctcgaa gcgcgggtaa tccggcaaca gccgcgtgta atgctcttta acgcgcagta
    18901 aaaatgccgc gtccgtactt tggccgttag taatgcagcg cagttgctcc acgaccagac
    18961 caacgtgatg atcgtaaaga tggatacggt ttttcatcgc ctgctggaca gcatgccagt
    19021 cggcctgttc gaaacgctgc tgcgccccgg aagtcacttc gaggaatcga ccatactgag
    19081 cgtcgaagcc ttgcaaaatg gtttgagcaa tcagtaattc caggccacgc ggcatttagc
    19141 gccctcatca ggagcagaga attgccggat gcggcgtaaa cgccttatcc agcctacgtt
    19201 cggcaacggt tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat
    19261 tgccggatgc ggcgtaaatg ccttatccgg cctacagtca gcaacgtttg taggttgctt
    19321 agaactgcga ttcttcagtg gagccagtca gcgcggtgac tgaagacgtg ccgccctgaa
    19381 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
    19441 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaaca tagtgcttca
    19501 taccttcgcc ctgcgcataa gcgtttgcca ggtcgaacat gttaaaccac atgctgtgga
    19561 tacctgctag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
    19621 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
    19681 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
    19741 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
    19801 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
    19861 cggtaataaa ttcgctgtca tacgggtcgc aatctgaggt gatcagatcc gccgcatcag
    19921 catcggtacg tgcaaccagc agggttggaa cgcccgtcac gtcagctgcc agacgcgccg
    19981 cgaccagttt ctgaatagct tcctgagttg gcactaaaac tttgccgccc atgtgaccgc
    20041 atttcttcac tgacgccagc tgatcttcga agtgaactgc cgcagcaccg gcttcaatca
    20101 tcgctttcat cagttcaaag gcattcagga caccgccaaa accggcttcc gcatcggcaa
    20161 cgatcggcag gaagtaatcg acatagcgcg gatcgcccgg ctcaatgccc acggaccatt
    20221 ggatctgatc ggcacgacgg aaggtgttgt tgatccgctc caccacagct ggcaccgagt
    20281 ttgccggata gagcgactga tccggataca tgctggccgc caggttagcg tccgccgcta
    20341 cctgccatcc cgacagatag actgcttcaa tacccgcttt cgcctgttgc agcgcctgac
    20401 cgccagtcag tgcaccgagg ttgttgatgt agcctttttt cgactcaccg tgcagcagac
    20461 gccacatttt cgctgcgccc agttgcgcca gcgtgcattc aggattgact gaaccgcgta
    20521 atttcaccac atcttccgca ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc
    20581 actctttctg taattcttca atttgttgtg tacgggtttt catgtgcaga tgctccatag
    20641 ttatgtggtg gtttacgcta acaggcggta gcctggcagg gtcaggaaat caattaactc
    20701 atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg tcaaaacggc cctgggagaa
    20761 acgttcttcg cccagttcgc tggcaatgac tttcatctct tcgcccagca tctggcggaa
    20821 caaggctttg gtcaccggtt tgccattgct caacgttttt tgatgatgga tccactgcca
    20881 gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc atcaggccat aaatcggcac
    20941 gcagccgttg ccggagatcc acgcttcgat gtactgcaca gccacgcgaa tgttagcgcg
    21001 cataccttct tcggtgcgtt caccatcaca aggtgccagc agctgatcgg cagtaatcgg
    21061 cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg gagccgagaa tgtcgttgaa
    21121 taccgccatt gccgtgtccg caaggcctgg gtgagcgatc catgtgccat cgtgaccgtt
    21181 attggcttcc agcgctttat ccgcttttac tttgttgagc acctggttat tacgctcttc
    21241 atctttgctc ggaataaacg cggccatgcc gcccatcgca aaagcaccgc gtttatggca
    21301 ggttttaatc agcaggcgtg agtaagcatt caggaatggt ttatccatcg tcacggcctg
    21361 tctgtctggc aggacacgat cgggatagtt tttcaacgtt ttgatatagc tgaagatgta
    21421 atcccagcga ccgcagttca gaccaacaat atggtcgcgc agcgcgtgaa ggatttcgtc
    21481 catctggaac acggcgggca gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg
    21541 cagattaaag cgatcttctg catagctgaa gacttcgctc caccaggccg cttcctgcta
    21601 ggactgggtt ttcggcagat agaaataggg accgctgccc tttgccaaca gtgcctgata
    21661 gttgtggaag aaatagagcg caaaatcaaa caggctaccg gggattgcct caccacgcca
    21721 ggtgacatgt ttttccggca agtgcagacc gcgtacccga caaatcaaaa ccgctggatt
    21781 gggcttgagc tgataaattt tgcctgcttc attggtatag ctgatggtac cgttaaccgc
    21841 atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag tctggtgcca gtgaatcttc
    21901 gaaatcggcc ataaagactt tcacattggc gttcagtgcg ttgatcacca tcttgcgctc
    21961 taccgggcca gttatctcta cgcggcggtc ttctaagtcc gcaggaatcc cgcgaatttt
    22021 ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa tcaggcaacg ttccgttatc
    22081 aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg
    22141 cgtcaccagc tcagtcagaa attctaccgc ttcggcagta agaatttgct tctcctgctc
    22201 gccatacggc cttgtgaaag ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca
    22261 gctcctcgtc atggatccga aaacttcccc tctgtgaacg aaggaacgtt gtgcgctttt
    22321 cgttcagcac aactaagact actcatttaa aatgcaaaat caaaaacaat ttccatttta
    22381 attaaaaata cttgataact tattgataac aatatgaata aaatttattc acttgctgag
    22441 gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt
    22501 aacgatcgac tatcacagaa gattaatcca gcgttggatt catgtgccgt agatcgtatg
    22561 gcgtgatctg atagacgtaa tagttgagcc agttggtaaa cagcaaatta ccgtgactac
    22621 gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg gaaatagtta tacggtacat
    22681 ccgggtctag tccggcttcc acatcgcgta aatattcctg cgccagcgtt tgcgcatcat
    22741 attcgggatg gcccgtcaca aaggcaatgc gcttatcttt gctggcaaac agatatgcat
    22801 ccccttcttc cgtctctgcc agaatttcca gatcggtgta atcacgaatc aacgctgccg
    22861 gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc atcaaagcca cgcgtcagaa
    22921 gcgcatgagg atggagaata tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt
    22981 gcttaggaat gccgtagagg atattgagcg cggcctgtac cgcccagcag acaaacagcg
    23041 tcgaggtgac gtgatctttc gaccactcca gcacctgttt gatctgcggc cagtaagcga
    23101 catcattaaa ctccaccagg cccagcggcg caccggttac aatcagaccg tcaaagttct
    23161 gttcctgaat atcttcaaag ttacagtaga agttgttcag atgctctgcg ggcgtgttgc
    23221 gcgattcacg ggaatcgatg cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa
    23281 gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg catcaggtta aggatcagaa
    23341 ccttaagtgg acgaatttcc tgaccagacg cacgagaagt tgtcatcaca aagacgtttt
    23401 cttcacgcaa gaaattgacg gcgggtagct cgtccggcac acgaatcggc ataacctgat
    23461 tacctcacta catacgctta tacgtttaga catccagata gctgaagata accagaaaat
    23521 ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt caaggaaaag gctacgttag
    23581 aatataagaa tgacgaaaag gagagaaaat ggttattagt attcgccgct cacggcatga
    23641 ggaaggggag gaactcgttg cgatttggtg tcgatctgtc gatgccaccc acgatttttt
    23701 atcagcagag tatcgggccg agctggaaga gctggtgcgt tcctttctgc cggaagcacc
    23761 gttgtgggtc gcggttaatg aacgggagca gccggttggg tttatgttgt taagtgggca
    23821 gcatatggat gcgctgttta tcgatcctga tgtgtgcggc tgcggcgtgg gtcggatgct
    23881 ggtggagcat gcgctctcga tggcaccgga actgacaacc aacgttaatg agcaaaatga
    23941 gcaggcggtt gggttctata agaaggtggg ttttaaggtt acgggacgct ctgaggtgga
    24001 cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat gtgggggaat aagccgcctc
    24061 tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DBBBSB010000063        23012 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.063, whole genome shotgun sequence.
ACCESSION   DBBBSB010000063 DBBBSB010000000
VERSION     DBBBSB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.063"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACIUS0_002402"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACIUS0_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGB0220123.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACIUS0_002403"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACIUS0_002403"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGB0220124.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACIUS0_002404"
     CDS             complement(3736..4092)
                     /locus_tag="ACIUS0_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGB0220125.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACIUS0_002405"
     CDS             complement(4096..4512)
                     /locus_tag="ACIUS0_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGB0220126.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACIUS0_002406"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACIUS0_002406"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGB0220127.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="ACIUS0_002407"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="ACIUS0_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="ACIUS0_002408"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="ACIUS0_002408"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGB0220128.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="ACIUS0_002409"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="ACIUS0_002409"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGB0220129.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="ACIUS0_002410"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="ACIUS0_002410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGB0220130.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="ACIUS0_002411"
     CDS             10521..11504
                     /locus_tag="ACIUS0_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGB0220131.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="ACIUS0_002412"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="ACIUS0_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGB0220132.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="ACIUS0_002413"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="ACIUS0_002413"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGB0220133.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14153)
                     /locus_tag="ACIUS0_002414"
     CDS             complement(13446..14153)
                     /locus_tag="ACIUS0_002414"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGB0220134.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14177..14431)
                     /gene="yjbL"
                     /locus_tag="ACIUS0_002415"
     CDS             complement(14177..14431)
                     /gene="yjbL"
                     /locus_tag="ACIUS0_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGB0220135.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="ACIUS0_002416"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="ACIUS0_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGB0220136.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="ACIUS0_002417"
     CDS             complement(15306..15515)
                     /locus_tag="ACIUS0_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGB0220137.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="ACIUS0_002418"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="ACIUS0_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGB0220138.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="ACIUS0_002419"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="ACIUS0_002419"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGB0220139.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="ACIUS0_002420"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="ACIUS0_002420"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGB0220140.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="ACIUS0_002421"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="ACIUS0_002421"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGB0220141.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="ACIUS0_002422"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="ACIUS0_002422"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGB0220142.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="ACIUS0_002423"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="ACIUS0_002423"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGB0220143.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..>23012)
                     /locus_tag="ACIUS0_002424"
     CDS             complement(22469..>23012)
                     /locus_tag="ACIUS0_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGB0220144.1"
                     /translation="DNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLVDKNNNFFIQLI
                     DQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFS
                     SVDDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKY
                     ALFPIFTASWRATRIKNKGI"
BASE COUNT         5932 a         5966 c         5520 g         5594 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cg
//
LOCUS       DBBBSB010000064        22442 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.064, whole genome shotgun sequence.
ACCESSION   DBBBSB010000064 DBBBSB010000000
VERSION     DBBBSB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22442
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.064"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            82..2682
                     /locus_tag="ACIUS0_002425"
     CDS             82..2682
                     /locus_tag="ACIUS0_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGB0220145.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACIUS0_002426"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACIUS0_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4300
                     /locus_tag="ACIUS0_002427"
                     /pseudo
     CDS             3754..4300
                     /locus_tag="ACIUS0_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4308..4823
                     /locus_tag="ACIUS0_002428"
     CDS             4308..4823
                     /locus_tag="ACIUS0_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0220146.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4789..5526
                     /locus_tag="ACIUS0_002429"
     CDS             4789..5526
                     /locus_tag="ACIUS0_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGB0220147.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5637..6647
                     /gene="pyrD"
                     /locus_tag="ACIUS0_002430"
     CDS             5637..6647
                     /gene="pyrD"
                     /locus_tag="ACIUS0_002430"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGB0220148.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6821..7363
                     /gene="zapC"
                     /locus_tag="ACIUS0_002431"
     CDS             6821..7363
                     /gene="zapC"
                     /locus_tag="ACIUS0_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGB0220149.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7360..8469)
                     /gene="ycbX"
                     /locus_tag="ACIUS0_002432"
     CDS             complement(7360..8469)
                     /gene="ycbX"
                     /locus_tag="ACIUS0_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGB0220150.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8713..10821
                     /gene="rlmKL"
                     /locus_tag="ACIUS0_002433"
     CDS             8713..10821
                     /gene="rlmKL"
                     /locus_tag="ACIUS0_002433"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGB0220151.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10833..12740
                     /locus_tag="ACIUS0_002434"
     CDS             10833..12740
                     /locus_tag="ACIUS0_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0220152.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12870..14123
                     /gene="pqiA"
                     /locus_tag="ACIUS0_002435"
     CDS             12870..14123
                     /gene="pqiA"
                     /locus_tag="ACIUS0_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGB0220153.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14128..15768
                     /gene="pqiB"
                     /locus_tag="ACIUS0_002436"
     CDS             14128..15768
                     /gene="pqiB"
                     /locus_tag="ACIUS0_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGB0220154.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15765..16328
                     /gene="pqiC"
                     /locus_tag="ACIUS0_002437"
     CDS             15765..16328
                     /gene="pqiC"
                     /locus_tag="ACIUS0_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGB0220155.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16584..16751
                     /gene="rmf"
                     /locus_tag="ACIUS0_002438"
     CDS             16584..16751
                     /gene="rmf"
                     /locus_tag="ACIUS0_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGB0220156.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16821..17339)
                     /gene="fabA"
                     /locus_tag="ACIUS0_002439"
     CDS             complement(16821..17339)
                     /gene="fabA"
                     /locus_tag="ACIUS0_002439"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGB0220157.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17408..19168)
                     /locus_tag="ACIUS0_002440"
     CDS             complement(17408..19168)
                     /locus_tag="ACIUS0_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGB0220158.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19353..19805
                     /gene="matP"
                     /locus_tag="ACIUS0_002441"
     CDS             19353..19805
                     /gene="matP"
                     /locus_tag="ACIUS0_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGB0220159.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19881..20927)
                     /gene="ompA"
                     /locus_tag="ACIUS0_002442"
     CDS             complement(19881..20927)
                     /gene="ompA"
                     /locus_tag="ACIUS0_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGB0220160.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVLGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21283..21792)
                     /gene="sulA"
                     /locus_tag="ACIUS0_002443"
     CDS             complement(21283..21792)
                     /gene="sulA"
                     /locus_tag="ACIUS0_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGB0220161.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22011..22421
                     /gene="sxy"
                     /locus_tag="ACIUS0_002444"
                     /pseudo
     CDS             22011..22421
                     /gene="sxy"
                     /locus_tag="ACIUS0_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5727 a         5267 c         5879 g         5569 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
     4321 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
     4381 gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
     4441 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
     4501 gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg
     4561 atgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
     4621 gtacggatgc agccagtggg ctggcaattg cgattctgga tgatgcgaag atattgattc
     4681 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
     4741 atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga
     4801 catttgtact tcattatgac taatacgtgg aacagattag cgctcttgat tttcgccgtt
     4861 ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg aacgcgattt
     4921 atctttccgg cagacagaga atcgatatct attttactga ctaataccag tcaggaatct
     4981 tggcttatta acagcaaaat caatcgccca acgcgttggg ccggggggga agcgtcgaca
     5041 gtgccagcac tattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc
     5101 acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt
     5161 gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg
     5221 atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa
     5281 gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg
     5341 ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag taatgcggga
     5401 gtcgtgccgc ctaaaagcca gcgtcagaca aactggtgtc aggtaatagc accgtgtcat
     5461 gtcgcctggc ggacgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg
     5521 ccctgaaaca ggttctgaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg
     5581 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
     5641 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
     5701 cttttcagca attacgccgc attaccggaa caccgtttga agcactggtg cggcagaaag
     5761 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
     5821 ccggtcttga taaagacggg gagtgcattg atgcgttagg cgcgatggga tttggatcga
     5881 tcgagatcgg taccgtcacg ccacgtccac agcctggtaa tgacaagccg cgtctctttc
     5941 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
     6001 acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cttgggtatt aatatcggca
     6061 aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa
     6121 tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac
     6181 gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
     6241 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
     6301 tttctgaaga cgaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
     6361 ttattgcaac caataccact ctcgatcgtt ctctggttca gggaatgaaa aattgcgatc
     6421 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag cacagaaatt attcgccgct
     6481 tgtcactaga attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta
     6541 tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta
     6601 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
     6661 ttcctcttta taagataatc agggctttat tttcagacct ggttgtttta tattcatcgt
     6721 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
     6781 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
     6841 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
     6901 tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc
     6961 aggcttttgt gttgatgatg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt
     7021 taatttatct aaagaccaaa aagccgaact ggtgctgaat gcactggtag caattcgtta
     7081 tctcaaaccg caaatgccga aaagctggca ttttgtctcc catggtgaaa tgtgggtgcc
     7141 catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct
     7201 ggtcgttgaa tctggcgaaa atgccgcgct atgcctgctg gcacaaccct gcgttgttat
     7261 tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc
     7321 gcaggtgaat gttgacagct tcagcctcga acaggcagtc taacgcgtca gcttaagggc
     7381 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
     7441 cagcggtgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
     7501 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cttgttggtt
     7561 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
     7621 gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag
     7681 aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc
     7741 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
     7801 gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca
     7861 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc tatcttcttc
     7921 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
     7981 cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
     8041 atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc
     8101 cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
     8161 tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
     8221 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
     8281 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
     8341 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag
     8401 agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
     8461 tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
     8521 tattagctat aatgcgcaac aattttctta gcgcctgtcc cgttaggcta ttttatttgt
     8581 cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
     8641 gcgctctcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
     8701 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
     8761 aaaactgaac tggaaaatct gggggccgtt gaatgccagg tggttcaggg tggggtccat
     8821 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt
     8881 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
     8941 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
     9001 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
     9061 gtcgatgctt tcacacggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
     9121 atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt
     9181 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
     9241 ctggcagccg ctattgtgat gcgatccggc tggcagtcag gaacaccgct gctcgatccg
     9301 atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcacca
     9361 ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg
     9421 caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctagctga gtatagctct
     9481 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
     9541 cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat
     9601 ccgctgccga aagggccgta cggtacagtg ttgagcaacc cgccatacgg tgaacgtctg
     9661 gacagcgaac cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag
     9721 ttcggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcctgcag
     9781 ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat
     9841 taccacgttg ccgaaagcac accagacagt aaaccggcaa tggtagcgga agattacgcc
     9901 aaccgcctgc gtaagaacct caaaaaattc gagaagtggg cacgccagga agggattgaa
     9961 tgttaccgcc tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttatgcc
    10021 gactgggtgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt
    10081 cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa
    10141 ctggtgctga aaacccgtga acgccataag ggcaaaaacc agtaccagaa actgggcgag
    10201 aagggcgagt ttcttgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat
    10261 tatctcgata ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct cggtcagatg
    10321 agcaaaggca aagatttcct caacctgttt tcttataccg gcagcgccac cgtgcatgcg
    10381 ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggaatgg
    10441 gcagaacgca acctgcgtct gaatggcctg actaggcgtg cgcatcgcct gattcaggcc
    10501 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg
    10561 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    10621 gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac
    10681 aacaaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa
    10741 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    10801 tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat
    10861 ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg
    10921 aacgtgtttg tctggtgggc cgcaacggcg caggaaaatc gacgttaatg aaaatcctca
    10981 accgtgaaca agggctggat gacggtcgca ttatttatga gcaagatttg attgtagctc
    11041 gtctgcaaca ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag
    11101 gcattgaaga acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga
    11161 acgacccgag cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc
    11221 acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc
    11281 ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg
    11341 cgctggtgag caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg
    11401 aaaccatcga ctggctggag gggttcctta aaaccttcaa tgggacgatt attttcatct
    11461 cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca
    11521 agctggtgac ctatccaggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc
    11581 gcgtggaaga actacaaaat gccgagttcg atctcaaact ggcgcaggaa gaagtgtgga
    11641 ttcgccaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag
    11701 cgatgcgtcg cgaacgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg
    11761 aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggtta
    11821 acggtaagca actggtgaaa gatttttctg cccaggttct acgtggcgac aaaattgccc
    11881 tgattggtcc gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc
    11941 aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc
    12001 accgcgcgga actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag
    12061 aggtgatggt taatggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc
    12121 atccgaaacg ggcgatgacg ccagtacgtg cgttttctgg cggtgagcgg aaccgcttgc
    12181 tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg
    12241 atcttgatgt cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg
    12301 tattgctggt tagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct
    12361 tcgaaggcgg cggtaaaatt ggtcgttatg ttggcggtta tcatgatgcc cgtggtcagc
    12421 aagaacaata tgtggcactc aaacagcctg cggtgaaaaa aagcgaagaa gccgccgcgc
    12481 caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac
    12541 tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc
    12601 aagtggcgga tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgcttgctg
    12661 atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag
    12721 cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg
    12781 taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct
    12841 gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca
    12901 tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag
    12961 cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc
    13021 ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg
    13081 tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc
    13141 ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc
    13201 ccgcgttttg tctgataacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt
    13261 taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt
    13321 tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc attggggtag
    13381 gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg
    13441 ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa
    13501 aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc
    13561 ttcctgctga tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa
    13621 acagcctgca gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta
    13681 atattttgcc catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg
    13741 ctggggtcat tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg
    13801 ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca
    13861 aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg
    13921 taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta
    13981 tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca
    14041 taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg
    14101 aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag
    14161 aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc
    14221 tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat
    14281 gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg
    14341 gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat
    14401 tccggtatgg aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt
    14461 ggtcgcgaag ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag
    14521 ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcacc accgttggcc
    14581 ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg
    14641 ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc
    14701 gatacgcaaa aacgcaatat cagttatcaa ctgttcatca atgcacctta tgaccgactg
    14761 gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagcg
    14821 gggatgcgtg tggagatggg ctcattgaca acgctgctta gtggcggtgt tagctttgat
    14881 gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat
    14941 gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc
    15001 tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc
    15061 ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat
    15121 gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc
    15181 gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg
    15241 ctgaaaaccg gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat
    15301 acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc gactgttagc
    15361 ggcggcttgg cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg
    15421 ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg
    15481 aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca gtcgatgcag
    15541 cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc
    15601 cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg
    15661 ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt
    15721 gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag
    15781 tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt
    15841 accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt
    15901 tatgggtaga gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa
    15961 ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac
    16021 agttgcgcaa caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct
    16081 cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag tttaacggtc
    16141 gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga
    16201 tcaaacgtcc gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta
    16261 aagtgctggc cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc
    16321 taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt
    16381 ttttgtttct aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg
    16441 cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat
    16501 tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga
    16561 aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac
    16621 atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga
    16681 cgctgagtca aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag
    16741 taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt
    16801 gatactctgt ctgatgataa tcagaaggca gacgtatcct ggaacagacc gactttcagg
    16861 tcgctggcgg tatagatcag acgacaatca accagcactt cgccatctgc cagacccata
    16921 atcagacgac gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc
    16981 ggcagtacct gaccagtgaa tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg
    17041 ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat
    17101 cccggcataa ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata
    17161 tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc
    17221 acacggtcta tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt
    17281 tcaccgtgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg
    17341 ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca
    17401 agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac
    17461 gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt
    17521 ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta
    17581 ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta
    17641 aatggcgaac gttagcagtg gggataatga caccttgttt ccccgttaac tcacgttgct
    17701 ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc tggacgcggc
    17761 cgaactgatc gactgaacct gtgatagcga tactctgatt cactggcaca tcagcgaggg
    17821 cgcttatcag ggcgcagagt tcagccatcg aggcactatc gccatccact tcactgtatg
    17881 actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca
    17941 tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt
    18001 tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag
    18061 gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc
    18121 cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac
    18181 gttcagcgag gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc
    18241 cggagaaggt gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact
    18301 gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta
    18361 ccggccaagc atccgctccc ggggcaggta agtgattatg tctggcggta aatgtcaccc
    18421 agcgacacca ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc
    18481 tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc
    18541 gttcgcctac cagaatgact ttcagcttca atggcattga aggcacagag acggggagag
    18601 ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc
    18661 gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat
    18721 ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc
    18781 caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat
    18841 tatcatctgc actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgat
    18901 cgcccaccag ttggcctgca tcgctttgta gcgtacgcgc ggcatcggca ataagattca
    18961 gatactcaga ctcttccggg gccttagcca gcataaaaga ggaggatgct cgcgtatgca
    19021 gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt
    19081 cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt
    19141 cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg
    19201 cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcctggctg
    19261 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    19321 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    19381 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaattaccc
    19441 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctcgaaaatg
    19501 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    19561 tgaagcagac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    19621 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcacagc
    19681 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    19741 agaaatacgc gaataaaatg tcttctctta agcaggatct gcaggcattg ctgggtaagg
    19801 aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc agcagcgggg
    19861 tttttctacc agacgataac ttaagcctgc ggctgagtta caacgtcttt gataccttta
    19921 acttcgatct ctacgcgacg atccggagcc aagcagtcga tcagtgcagc acgctgtttc
    19981 acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc
    20041 ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg
    20101 gacagaccct ggttgtaagc gtcagaaccg atgcggtcag tgtaacccag aacaactacg
    20161 gaaccgtctt tcggatctag gttgctcagc tggctgtaca gctgatccag agcagcctga
    20221 ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag
    20281 tgcttggtct gtacttccgg tgccggagct ggagcaacta ccggagctgc ttcgccctga
    20341 ccgaaacggt aggaaacacc caggctcagc agaccgttgt ccggacgagt accgatggtg
    20401 tttgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    20461 atcgcgtact caacaccacc tgcgaaaacc ggagaaacgc cagtgtcgtg gtctttaaag
    20521 gatgcgccac caagtacgtt agccttggtg tctgcacgcc ataccatacc acccagacga
    20581 gtgtagatgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    20641 tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc taaccagtcg
    20701 taacccattt caaaaccaac atacgggtta acctggtaac caccaaaagc acctgcaccc
    20761 agttggtttt cgtgggtcgg accattgtta ggaataaaac cagtgtcatg gtactgggac
    20821 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    20881 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    20941 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    21001 gggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctggc
    21061 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    21121 aaaaaagtct tgtataaggt atgtttaatc tttttgtcag cgacaattta cagaaaaaaa
    21181 acgcggaaac cgcttcagac aagccttcgc aaggaaaatt agtcacgact gaaagcattg
    21241 gctgggcgac aaaaaaagtt ctaggattaa tcctaaattt acttaatgat acaaattaga
    21301 gtgaattttt agcccggaaa gttgtctcgt tgtgtgagag gatgcgctta ccggacgcat
    21361 aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc
    21421 agtcaaatca tctgtcaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg
    21481 aaccattgac tccactgtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa
    21541 gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag
    21601 ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat
    21661 catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt
    21721 agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc
    21781 tgaagtgtac ataatcaatc cagcccctgt gagttactgt atggatgtgc agtacatcca
    21841 gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac
    21901 gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg
    21961 atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc
    22021 tctcctataa gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat
    22081 accgatcatt gtttggcagt tacagcctga ccgttgacga cacggtgttt gcgatggttt
    22141 ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc
    22201 cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg
    22261 ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg
    22321 atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca
    22381 atatgtcttt tcatctggaa gcgattctcg gggaggtggg tagactggcc ccctgaatct
    22441 cc
//
LOCUS       DBBBSB010000065        21634 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.065, whole genome shotgun sequence.
ACCESSION   DBBBSB010000065 DBBBSB010000000
VERSION     DBBBSB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21634
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.065"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(15..905)
                     /locus_tag="ACIUS0_002445"
     CDS             complement(15..905)
                     /locus_tag="ACIUS0_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HGB0220162.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(935..2191)
                     /gene="wzx"
                     /locus_tag="ACIUS0_002446"
     CDS             complement(935..2191)
                     /gene="wzx"
                     /locus_tag="ACIUS0_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HGB0220163.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2207..2752)
                     /gene="rfbC"
                     /locus_tag="ACIUS0_002447"
     CDS             complement(2207..2752)
                     /gene="rfbC"
                     /locus_tag="ACIUS0_002447"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGB0220164.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2757..3635)
                     /gene="rfbA"
                     /locus_tag="ACIUS0_002448"
     CDS             complement(2757..3635)
                     /gene="rfbA"
                     /locus_tag="ACIUS0_002448"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGB0220165.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3694..4593)
                     /gene="rfbD"
                     /locus_tag="ACIUS0_002449"
     CDS             complement(3694..4593)
                     /gene="rfbD"
                     /locus_tag="ACIUS0_002449"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGB0220166.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4593..5678)
                     /gene="rfbB"
                     /locus_tag="ACIUS0_002450"
     CDS             complement(4593..5678)
                     /gene="rfbB"
                     /locus_tag="ACIUS0_002450"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGB0220167.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(6051..6944)
                     /gene="galF"
                     /locus_tag="ACIUS0_002451"
     CDS             complement(6051..6944)
                     /gene="galF"
                     /locus_tag="ACIUS0_002451"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGB0220168.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7119..8513)
                     /gene="wcaM"
                     /locus_tag="ACIUS0_002452"
     CDS             complement(7119..8513)
                     /gene="wcaM"
                     /locus_tag="ACIUS0_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGB0220169.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8524..9744)
                     /gene="wcaL"
                     /locus_tag="ACIUS0_002453"
     CDS             complement(8524..9744)
                     /gene="wcaL"
                     /locus_tag="ACIUS0_002453"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGB0220170.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9741..11021)
                     /gene="wcaK"
                     /locus_tag="ACIUS0_002454"
     CDS             complement(9741..11021)
                     /gene="wcaK"
                     /locus_tag="ACIUS0_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGB0220171.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11201..12679)
                     /gene="wzxC"
                     /locus_tag="ACIUS0_002455"
     CDS             complement(11201..12679)
                     /gene="wzxC"
                     /locus_tag="ACIUS0_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGB0220172.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12681..14075)
                     /gene="wcaJ"
                     /locus_tag="ACIUS0_002456"
     CDS             complement(12681..14075)
                     /gene="wcaJ"
                     /locus_tag="ACIUS0_002456"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGB0220173.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14130..15500)
                     /gene="cpsG"
                     /locus_tag="ACIUS0_002457"
                     /gene_synonym="manB"
     CDS             complement(14130..15500)
                     /gene="cpsG"
                     /locus_tag="ACIUS0_002457"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGB0220174.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15693..17129)
                     /gene="cpsB"
                     /locus_tag="ACIUS0_002458"
     CDS             complement(15693..17129)
                     /gene="cpsB"
                     /locus_tag="ACIUS0_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGB0220175.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17132..18355)
                     /gene="wcaI"
                     /locus_tag="ACIUS0_002459"
     CDS             complement(17132..18355)
                     /gene="wcaI"
                     /locus_tag="ACIUS0_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGB0220176.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18352..18831)
                     /gene="gmm"
                     /locus_tag="ACIUS0_002460"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18352..18831)
                     /gene="gmm"
                     /locus_tag="ACIUS0_002460"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGB0220177.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18831..19799)
                     /gene="fcl"
                     /locus_tag="ACIUS0_002461"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(18831..19799)
                     /gene="fcl"
                     /locus_tag="ACIUS0_002461"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGB0220178.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(19802..20923)
                     /gene="gmd"
                     /locus_tag="ACIUS0_002462"
     CDS             complement(19802..20923)
                     /gene="gmd"
                     /locus_tag="ACIUS0_002462"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGB0220179.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(20949..21497)
                     /gene="wcaF"
                     /locus_tag="ACIUS0_002463"
     CDS             complement(20949..21497)
                     /gene="wcaF"
                     /locus_tag="ACIUS0_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGB0220180.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21513..>21634)
                     /locus_tag="ACIUS0_002464"
     CDS             complement(21513..>21634)
                     /locus_tag="ACIUS0_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGB0220181.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKAVYNKV"
BASE COUNT         5384 a         5715 c         5004 g         5531 t
ORIGIN      
        1 ttttttgatc agaactatgc attataacga ccgcccccag taattcctct tattccatcc
       61 agcgtagcca aaatatgttt tttcatatat tcaattttat ttttttgagt tgcgataatt
      121 aaagtcgtat gaattaaaga aaaaacaact tccctaatag agagtaaaag aggcacatga
      181 ttttttctaa gcaaaagaaa tgcatttctt actcgataat aacgacgtac tggcgagtga
      241 attggtatgt taatcccaca gatttttact gacttatccc caatagcatg aaccatttct
      301 atatcaggaa tgatatgaac taatatgcca tagtttaagc atctaagaca ccattctgta
      361 tcgacataat caataaaaag ttcctcatcc atcattccaa caatctcaaa aacttctttt
      421 gaaaccatgg ttccggatga gattgttact gaacttttaa acggtgtctg tccttcagtg
      481 acatgaattt tctctcgaag accatttttt ttgatattac aaataggata ataatgtgac
      541 ttatctctat caataaaaac tgggccagct atttttgcat tttcattatc catatagcag
      601 gaaagttttt tgataaactc attgcgaata cggctatctt gatcaaaaaa aattatcttc
      661 tctgcccctt tttcaagaga atatcgaatc ccaatatttt gtgcttttgc tataccttca
      721 ttcctaccaa gacaaattaa atgaactttt tcaatgtctg ctaatttatc tttcaaggtg
      781 aaattagtcc tattatcgac tactacaacg taacaatttt gctcctttag ttcagtgatc
      841 agtgcattca gatttttaag ttcgggatta tatgtcacaa tgacagcgta aatattacta
      901 ttcattatcc aagtgactca gtaattggtt aattttagtt ttcatataca gaacacattc
      961 tagtataaag ccattgtaat aacagagcat ctaaaattac acgtatgctc caggatattg
     1021 cggcacccag taacccatat ttttcgacca taaaatacaa aagggctaaa taaggaacca
     1081 attcagcaca atgaatcaaa gcagtaattt tagattttcc taatgcctgg attgcagaga
     1141 atggtatcat tgccagcgca ttgaagaaaa aaccgatcaa taacacattt aatatattaa
     1201 aggaattaat ttgataattc tcaccaaacc atattttcaa tacaagccct gaatataaca
     1261 agcctatgat tatcaccggc aaacagatta gaaacattaa aagcaatgat ttatttacat
     1321 tacgtttaaa ttctttaaag tctttgatgt tacttaacct tggaaacact gccctcccga
     1381 ttgctgcagg tattattcct aattttagaa taacctccgc tggtgcagaa taaaatgcaa
     1441 ctttatctgc ccccataata tttgacacta taaatcgatc aaaatacacc atgaccggac
     1501 ttatgatatt acttactgtc atccaaccgc caaagaaaaa gagtcgttta aaggttttat
     1561 aacaaaaatg aacccccgaa attttaatat cattcctgac ataataggca gagactaaaa
     1621 tcgaaataac tcttgcaaat attaaaccag caaccgctgc cgacaatgta gcactataaa
     1681 aaacaaatat ggccggaatt cccgcgatgc aagagcttga tatagatttt tgaatattta
     1741 caatgccaaa tttttcatcc ccctcaagaa tggctgacca taattgattt agaataaata
     1801 atggaatgca aatagccaac agtttaaatg ctagttgtat atcactatgt tcaacaccag
     1861 aaatttttag ataattaaca attccatcag agaaaatcaa taataaaaaa gcaccaaagc
     1921 atgaaaatag caccaaaaaa gatgtacttg ttgaaattac cttggttctt tcatgatgat
     1981 tatcacgatg aattgcaatt tctctaataa cagagcgagt caggcctaca tcaaaaattc
     2041 cagcataccc aacaagtgca attgcaattg tataaacacc gaaaccttca ggccccaaac
     2101 ctcgagcgag aaatcctagc gaggggatag caattaaagt tggtattgca tagccaaaaa
     2161 ggttccagac actatttttt attatgctca ttattacatt tctttatcat ataaaataat
     2221 cagcatctac aaatttttga ccattaatat ctttatcaga aagcaatgga ttttgaaccg
     2281 gccattctat atttatactt ttgtcgctcc ataaaatccc acgctcttgc atttttgaat
     2341 agaaattatt tgttttgtag acaaattctg cttcatcact aagaacgcaa aacccatgag
     2401 cgaatccttc cggtatccat aattgacgct tattctccgc cgacaaattt acaccaaccc
     2461 acttgccaaa tgttggtgat gatttccgaa tatcaacagc tacgtcaaaa acttcaccga
     2521 caacacagcg taccagtttt ccttgagcat aaggttccag ctgatagtgc agtccgcgta
     2581 acacaccctt agttgattta gaatggttat cctgaacaaa ctcaaccttt cgtcctacag
     2641 cctcttcgaa aactttctga ttgaagcttt ccataaaaaa gccccgctca tcaccaaata
     2701 cttttggttc aaaaattagc acatcaggaa tttcagtttt aattacgttc attttattaa
     2761 taacctttaa tcattttcag cagatactga ccataagcat ttttcttcag cggctcggct
     2821 aataccttca cttgctcagc atcaataaag cctttacggt gagcaatttc ttccgggcag
     2881 gaaactttca gcccctgacg ctcttcaatt gttgcaatga agttgcttgc ttcaataaga
     2941 ctttgatgtg tccccgtgtc cagccatgca taaccacgcc ccatcatagc gacagacaaa
     3001 cgtccttggt ccatataaat acggttaata tcggtaattt ccagttcgcc acgtgcagat
     3061 ggtttaaggt ttttcgccat ttctacaacg tcattgtcat agaaataaag cccagtaacc
     3121 gcatagttac tttttggttc ctgcggtttt tcttccagac taattgccgt accgttatca
     3181 tcaaattcca ccacaccgta gcgctcagga tcatttacgt gataggcaaa aaccgttgcg
     3241 ccactctctc tgttaacagc ggtatccatt aatttcggca ggtcgtgacc atagaagata
     3301 ttatcaccaa gtaccagcgc acaatcatca ccacctataa attcttcacc aatgataaat
     3361 gcttgcgcca gaccatccgg actcggttgt actttatatt gcagattaag cccccactgg
     3421 cttccatcac caagcaattg ttgaaaacgt ggtgtatcct gcggcgtact gataatcaga
     3481 atatctcgaa tacccgccaa cataagcgtt gaaagcggat aataaatcat cggtttatca
     3541 taaatcggta gcagttgttt gctcacagcc attgtcacag gataaagacg agtgccggaa
     3601 ccaccagcca gaataatacc tttacgcgtt ttcatttcac cattcctttt aattcatctc
     3661 gctctagcat catgagcgag atgcaataat ttgttaaatt gccgtggtcg taaataattc
     3721 gttgagcata cgtttcacgc ccacctgcca gtcaggcaag acaagcgcaa agttctgctg
     3781 aaatttttcg gtattgaggc gagaattatg gggacgacgt gctggtgtgg gataggccgt
     3841 tgtcggcacg gcattaagtt tgttaagagc aaggttgatc cccgctctgc gcgcttcttc
     3901 aaataccaga gcggcataat cgtgccaggt tgttgtacca ccagcaacca gatggtacaa
     3961 accagcaact tctggtttat ttgcggccac gcgaatggca tgagcggtgc aatcagccag
     4021 taattcagcc cccgttggtg cgccgaattg atcgtttatt acagccaact cttcgcgttc
     4081 ttttgccagg cgcaacatcg ttttggcaaa gttatttcct ttaccagcgt atacccagct
     4141 ggtacggaaa ataagatgct ttgcgcaatg ttcttgtaac gctttttccc cagctagctt
     4201 ggtttcaccg tagacattta gcggtgctgt tgcatccgtt tccagccatg gcgtgtcacc
     4261 gttgcctggg aaaacataat cagtggagta gtgtataacc caggctccga cttcattagc
     4321 tgcttttgca atcgcttcga cgcatgtcgc gttaagtaat tgcgcgaaat tcggttctga
     4381 ttccgctttg tctactgcag tgtgagcagc agcattaaca ataacatcgg ggcgaatttt
     4441 tttgacggtt tcagccacac cttcggggtt gctgaaatct ccacaataat cagtggagtg
     4501 aacatcgagc gcaatcaagt tacccagagg agccagagca cgctgcagtt cccaacccac
     4561 ctgccctgtt ttgccgaaaa gcaggatatt cattactgac ggccctcata gttttgttca
     4621 atccacgact gataagtacc gcttttcaca ttctcaaccc aatttgtatt agccagatac
     4681 cattccaccg ttttacgaat cccgctctca aacgtttctt gcggtttcca gcccaattcg
     4741 cggctaattt tatcggcatc aatggcgtaa cggcggtcat gccccgggcg atcggcaaca
     4801 taagtaattt gctcacgata agatttctct ttcggtacaa tctcatccaa caaatcacaa
     4861 atagtgagca ctacgtcgat gtttttcttt tcgttatgtc caccaatgtt ataagtttca
     4921 cccgctttac cttcggttac gacggtatat aacgcacgag catgatcctc tacatacaac
     4981 cagtcgcgaa tttgatcccc tttaccataa ataggtaatg ccttaccttc cagagcattc
     5041 agaataacca atggaataag tttttccggg aagtgataag gaccataatt gttcgagcaa
     5101 ttagtcacaa tggttggtaa accataagtc cgcttccagg cacgtaccaa atgatcgctg
     5161 gaagctttag acgcagaata cgggctactt ggcgcgtaag ctgtcgtttc cgtaaatagc
     5221 ggtaacgctt cgttattatt tgcttcatcc ggatggggta agtcaccata aacttcatca
     5281 gtagaaatat gatgaaagcg gaaacttttt ttcttttcat cattcagagc agaccagtaa
     5341 ttgcgcgccg cttctaaaag aacataagtc cccacaatat tggtttcaat aaatgccgca
     5401 gggcccgtta ttgaacggtc aacgtggctc tctgccgcca ggtgcatcac cgcgtctggc
     5461 tggtgctgtg cgaaaatacg cgacatggct acagcatcgc agatatctgc atgctcaaaa
     5521 gcataacgtt cagaatcaga aacatcagcg agtgattcca ggttcccggc gtatgttaat
     5581 ttatcgacat taacaacact atcttgcgta ttatttatta tgtgacgaac aacagcagaa
     5641 ccaataaacc ctgcgccacc agtaacaagt atcttcactt ttttattcca tatagctata
     5701 gagcatgctg tgaaatagac tgctccagat ttgattaata gaggtattaa tacacgctac
     5761 cgcccctggc ttaacagcta ccagtgcact gcatgcatct caacgatgtg gcgagcgtta
     5821 gcctctcgcg ccaaatccga aaaattcaaa cgttaattgt cttaccaaac agccgtagaa
     5881 acaaggaaaa tcctggaaaa atttgaataa tatgagctat gtaacttctt gtttttatag
     5941 gctttataaa aacggcaacg gcaggaaaat tcaatgagca aaacttatgc cacgaatctt
     6001 cactgccgtt ataattttct tatcaaccgt tacatccggt cagattttca ttattcgctt
     6061 aacagcttct caataccttt acggaacttc gccccttctt tcaggttgcg taggccatac
     6121 ttcacaaacg cctgcatata gcccattttt ttgccgcagt cgtaactgtc gccggtcatc
     6181 agcattgcat caacggattg ttttttcgcc agctcggcaa tagcatcagt cagctgaata
     6241 cgtccccatg caccaggctg agtacgttcc agttccggcc aaatatcggc agaaagcaca
     6301 tagcgaccta cggccatgat gtctgagtcc agcgtctgcg gctgatccgg tttttcgata
     6361 aattcaacaa tgcggctgac tttaccctca cggtccagcg gctctttagt ctggatgacg
     6421 gagtattcag agaggtcacc cggcatacgt tttgccagca cctggctgcg gcccgtttcg
     6481 ttgaaacgtg caatcatggc agcaaggttg taacgtagcg ggtcggcgct ggcatcgtcg
     6541 atcacaacgt ctggcagtac cacgacaaat gggttgtcac caatggcagg tcgcgcacac
     6601 aaaatggagt ggcctaaacc taaaggttcg ccctgacgca cgttcataat gatcacgccc
     6661 ggcggacaga tggactgtac ttccgccagc agttgacgct tcacgcgctg ctcaaggagt
     6721 gattctaact cataagaggt gtcgaagtgg ttttcgaccg cgttcttgga cgcgtgagtt
     6781 accaggagga tttctttgat ccctgcagcc acaatctcgt caacaatgta ctgaatcatt
     6841 ggcttgtcga cgattggtag catctctttg ggtatcgcct tagtggcagg caacatatgc
     6901 atcccgagac ccgctacagg aataactgct tttaaattcg tcattatttc atccacctgt
     6961 aaaatggttg ctgaattata gcttgttcga tttttttcgc cagcatcaat taccctgaat
     7021 tgattactga attacttgtg atgttacgcc gcttcgttgt ggattgcagt agcattgttc
     7081 ctaagtatgg ctccattttt ccaggaatgg tcgcaaatct actccctccg ttccggcaat
     7141 ctaaagttaa tcttttccac attaacaata tggtgattaa tcctgtcgat atcgacggag
     7201 ctttgccctc tttcattcac cgcatgaaca tttgcaagag acagcagtgt ttcttttttc
     7261 gccataaaga cgccgcgaac gtctttgcgc atgtcgaagt tcatgctcaa tgctggtcca
     7321 actgaggatt cctgcatcac attgatattt cgcataaaaa gatgttgcgg cttgttgtgt
     7381 aactccagcg aggcacgctt catctcaatg ttagttagcg acacaaagga gacggcattc
     7441 ccggcggaga tttggatgcc gcgcaattta taagcaagat gggtattatc cagttgaata
     7501 ttattcactc ggaaattttg cggtatcgag agatatttgc ctttaattac cccatagccg
     7561 attaacatcc cggcgctatt aatcatttca atattatcaa tcacgaaatt gtcacaaccg
     7621 taaatagcga ctgtcgcgtt atcaatgccc gctttcttac tgaaatccgg cgtgatattg
     7681 cgggcattga tattacgaat aacaaaatgt ttaccatttt caacatggat caactgccga
     7741 caatccgatc ccgtgatatt cgccacgaca aagtttttca ctgcctggtc ttccgggtag
     7801 ttgttatcat aagtgcttcc cgcaaggcct atgccgatgc cccagttgat tttgccgttg
     7861 gtacagttga tacgctcgat gacatggtca gatatcaaaa tatcactgtc gttaattgcc
     7921 acgttccatt caatggcgtc gccctgtaag tcgctgaact tacagttggt aatgttggca
     7981 ccgataatct ggttatgaaa tccctggcgt aagatggcgt aattagcgtg gctgacagtg
     8041 aggttatcga tggtcaggtt gcgcatgacc cgtttgtttt tgccgccaat ataaatctgc
     8101 attaccgggc caaagccgct catcgccagc cctttgatgg tgcagtcaga accacgcaca
     8161 tccagggtaa tgttatgcat accgccgccc tctccccctg tcacctggct gccgtcctgt
     8221 aagacaaatc gccctctgcc gttgccgcgc aggcttccaa ggatgtgtaa cgttttgccg
     8281 ggagggatga agatgccggt gttgatattg tcacaaacga atccggcagg cacgacgacc
     8341 gtttgccctt cgctgaaggc ttgtttaaat gaggcgatcc agtcatgtgg gttgtagtcg
     8401 ttaatgttaa cgctttgtcg ggcgggaagt gcgcgagcga aaggggtatg gaggaaggca
     8461 agcgccgagc ttgccgtcag gaaggtgcgt cgggagagtg ttttaaatgg catacgttct
     8521 cctctataaa gcctgcagca agctggcgag ttctcgatta atcacctgct ggttaaaatc
     8581 gtgttcgact ttttcgcacg cacgtttgac gaccggagcc agttcgtcgg tatccagttg
     8641 gctaaacgcc gccagacgtt gtgccagtgc gcgagcatcg ttctcaggca gcagccagcc
     8701 ggatttatcg gcctccacca gttcaggaat tccgctatgc agagtagaaa ccaccggaat
     8761 accgaccgcc atcgcttcca ttagcgccac cggaatgcct tccatatcgc catccgcgcc
     8821 cgttaccgat ggcaacagga agacatccgc gtcgtcgagc atctccttca cttcatggct
     8881 cggtttaaag cccggcatct ctaccacatc ttccagttga tattgttcga tgatggtgcg
     8941 caggcgtcgt tcccacaggc caatgccgag gatgcgatag cgaaatgcca cgccctgctc
     9001 tttcaactga cggcaggctt caatcgccac atgcaggcct tttttctcgg ttaagcgtgc
     9061 gacagatatg atttccagcg gcgttgcggg cgctttcacc ggacgcgggc taaagcgcgt
     9121 catgtccacg cccatacgcg atacggcgat tttttctctg gggcagccca ttttttgcag
     9181 ccttccggcc cacagatcgc ttatcggtaa catcaggtcg ccacggcgaa acagttgttg
     9241 atattcggga gtgtagtggt tgagcacttc ccgactggag atatcaatac cgtggaagat
     9301 ggtggcaatt ttgccgcgaa tgatacccag ttcgcgtagt tttgctgcgg ttacccccgc
     9361 cgggccaaaa tgagcgatga acacatcggc gcgaaacggt gttgcgacct gaccacaaat
     9421 ggcagacaaa atcaggttcc gcgactcggc accatagcgt ttgaggttga gcgcatgcca
     9481 ggtattttta cgatgaatgc cacgtaaggt ctggctggcg cggtggcgca gtttcgctac
     9541 tttgcccgca ggttcgtcct gtaaccagcg ggttctggcg acaaggttgt atttcgtcca
     9601 tgccgcgtgg gtgttctctg tatcgccttt ttgcaaagcg acaatctcca cttcaaagcc
     9661 catatcaata aacgcggtaa tttgattgag gacgaaggtt tccgacgaca gcgggaattt
     9721 caataagaag aagccgacct tcatttcacc tccccgatgc gctcaagcac agactgcacc
     9781 atctgcattc ccgtctgacg ttcgcgactg acggcttcgt taaatcgagc gttcagcacc
     9841 ggaagctggc ctaaggtatc cgcaaccatc gcttgcaggc tgccgtccag taaatgacga
     9901 atatcaatcg ccatctctgg cagtcccagc tgctgcataa tcccggcgga tttatgttca
     9961 tagttgatgg caatcgccgg agtagcaaag ttcattgaga taatggcgga gtgcaggcgc
    10021 gtaccgacgg tgagttcaca ggctccgaga attttgccca tttccagatc gttgagttca
    10081 tccatcacta cgtggtaacg ggcaggatcg ctgatgtgct ggcgcagatt gagcgccacc
    10141 atgcggtcgt ctttgttata gctgtcgata cccgtacagg tggagagcgc aatcacctga
    10201 tagccttcat cgagaatgcg attgaccacc ccggcaaagg ctttttcata cgcttgttga
    10261 gtggtgccga gacgtttgtc gaacggtgcc agttcgcgca gggtaattgc cactgttttc
    10321 tgttgtgcgg caacgtccag ccagtgttga acggcatagc tggcggtgaa gtcttcggtg
    10381 tggtgatcga ccagccacgc ggtatcgacg ccatgttcca cttttgcggt ggtgatattt
    10441 ctgcgtttca tcaaatcaag gctgaccgat tcgcgcagga tcagcgcgtc gcagtgacca
    10501 aagacatagt tcgccagttg gttaaattgc tcatcctgga aggggccgac gctgtgacca
    10561 atcataaaca gcggcttttt cgccataaat gtgcaaagcg catgttcaaa ctgcggcacg
    10621 ccgtagagat cgacaaaaaa cgatccgccg acctgaataa tggcgtcgta acctgacagc
    10681 aggcgcacga agtcggtgaa tccctgggcg atggcgatat tgcgcagctt gccagtgtcg
    10741 gtgacgcgtg agagcaatac ctgatgctgg tagcggcgac ggaggacttt tttaatgcgc
    10801 ccgacaacgc ccgccgcgct attgtgttgt gtcatctgca ggaacagcgg atcgcccatt
    10861 accgggcggt tgagcagcca ggaagaactg accggatagc ggctcatcac gtccacttcg
    10921 gtatgtggat tgaggatgtt gatggcatca agtaagccgc gcaagatggc gctgtcgcca
    10981 cgattgccgc aagtgtggtt gcccagaata agtaatttca ttttttcctc ataaatttga
    11041 tgccaggtga ggctgtgttt gttgtgttcg ttgccgtttt ttgcctgatg cgacgctggc
    11101 gcgtcttatc aggcctacag gtcccgagca cagaaccgta ggacggataa ggcgttttac
    11161 gccgcatccg gcaaccgttg tcggaaccga aaacagcaat tcaccccgcc cgtaaaagca
    11221 ttttcatttt ttcgctgcga caaaactgac gcttcacttc caccaccagc ggatggcgtg
    11281 acagcacaat catcaccaca aacgccagca cgcccgcggc tatttgcacc gccagcagca
    11341 tccccagcgc cagttgccct ttcagcacaa tgcccagcgc ataactgacc accagcgtcg
    11401 gcaacgagag ataaaacggc agccacaaac tcaggatgta ctggcgataa ctggaaccaa
    11461 gaaccggttt aatcatcacg aagtaactca gaatggtgtt gataatctgc accagcagga
    11521 aaccgagcgt gacgccgatt gcgcccgcca tctgcccacc aataacaatc gccggaataa
    11581 acagaaatgt tttgaatacg ttgaatttaa agctgatatc gacccgcgct ttcgccatca
    11641 gcagcgaacc aatcgggtta cccacggagc gcagcagacc cacaatacac agcaattgca
    11701 gcaccggaat aatgctgttc cacttttcgc cgaataccag cggtacgaag ttattcgaca
    11761 ccaccatcag ccccagcagc gccggaaagt tgataatccc cactatcgac agcagcttgt
    11821 agaagttaac acgcagcttt tcggtgtcgt cctgaatttt ggcaaacgcc ggaaacaaca
    11881 cgcgggtgat gattgggttc agcttcatcg gcggtacgac ggccacgttg tacgccaggt
    11941 tgtatccccc tgccacgccc gcgccaagaa tacgcgccag caccagcgtt gaaaggttgg
    12001 tgttgagata gttgatgatg ctgtccgccg tcagccaggc accaaaacgt aagttcggtg
    12061 ccacagacgc cagcgagaag tgcagaccgg ggcaataaat tttgcgtcca aagtagccaa
    12121 acagcagtgt tctcaccgca ctattgacca gataaccgag gatcgcggtc atcgccagcg
    12181 gccagaaatg ggcgctaact accgtaaaag tgaagcccgc cagcaccgcg ctggtttcga
    12241 tcatgccgat tttgttgaac tccagctcct tctgcatcaa cgcgcggaac tgttgcccgt
    12301 gggggatcac cacaaacgcc agcgacaatg ttttaatcaa cggtgccagg tccgggttat
    12361 tcagcacgtc gccgatgaca tcactcaaca aaaacaccgc cacacatacc acaagcccca
    12421 gcccgacgtt cagccagtac agcgtggtga gttcaaggtg actgatttct tttcgctgaa
    12481 taatcgagtt agcgataccg aagtcagaaa gcgtatctgc cagcgcgata atcaccagcg
    12541 acacagtcag caggccgaac tggtggttgt cgataatccg cgccagcacg gtcatctgca
    12601 ccagcccgag gccgatgatg atcaccgtgg caatagccga ccacttcgcg ccgctgatgg
    12661 ttttttcacg taagctcata tcaatatgcc gctttgttaa cgaagccctt gaataccgtc
    12721 aggaaaacga ttttgatatc gaaccagacg ctccattcgc ggatgtactc aaggtcgaac
    12781 tcgacgcgtt tttccatttt ctccagcgtg tcggtttcgc cgcgccagcc attaatctgc
    12841 gcccagccgg taatgcccgg tttcacttta tggcgcagca tgtagccttc aatgagctgg
    12901 cgatactgtt cgttatgcgc tactgcgtgc ggacgtggac cgacaatcga catccccccg
    12961 gtcagcacat tgataaactg cggcaattcg tccagcgagg tgcggcgcag aaagttcccc
    13021 actttagtga cgcgcggatc gttctgcgtc gcctgggtca ccactttgtc gttctccatc
    13081 actttcatgg aacggaactt ccacactttg atcggcttgc catccatacc atagcgagtc
    13141 tggcggaaaa taaccggccc tggtgaactg agtttcaccg ccagcgcaat acagcacagc
    13201 accggggaga tcagcagcag aataagcgtc gccagcacaa tgtcttccgc acgttttagc
    13261 aggcggttaa cccccgaaag cggcgtgtca tacagcggca ccaccggaac gccgttcatc
    13321 tcttcgagac gtgaatggag aatgttgaag gtaaagacgt cggggatcag cagcaccgaa
    13381 caggtggtat ccgccagttg atggaccagt tttttcactc gtgcgccgtc gcacatttgc
    13441 atcgcgatat agacgttatg aatcttgccc gcttttgcat cctcgaccag ctgttgcagg
    13501 ttacccgccc agtcgttaga aacgccgccc ggtttcgggt cgtggtaaac gcccaccact
    13561 tcaaacccta accacggctg attacggaaa ctctccatca gcatttgtcc ggcggctaaa
    13621 tcccccgcta cggcgaccat ccgcttgttg tagccatgat tacgcagcca gcccgcccca
    13681 atgcgaatac atgaacggca aaccaccagt ccgatgctgg tcagcccata ccacgccagc
    13741 cagattttca gttgcgtgtc gaaatcattg ttgaacgcca ccagtccggc gctgaaaatc
    13801 acgcttaagg tccagttttg tagcaacagg gcaaattctg tcgctgcccg aacaccgcgc
    13861 catgagcgat aaaaatcggt gatgccgccc agcatctgga acaccaccag cgtaatcagc
    13921 gccaccaaca ggtgcatgta gaggaatgac agtccgctga cttcgcaaac cagccatagc
    13981 ccggcaaaca tgatggtgat atctgaaaag cgttgcacca tagagattaa cgatgcattg
    14041 gttttcgctc gctcgcgctt ttttagattt gtcatcgttg ttcctgttat tagcccctta
    14101 cccgcagtgg ggtaagggaa gatccgacat tactcgttca gcaacgtcag cagagttcgc
    14161 gttcgcgctt ccatcagcgg cacatcaccg cgcgattcca cattcaggcg caccaccggt
    14221 tcggtattgg aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg
    14281 ctgcgatcca ccgccagcgc ctcacggcta aaatgctgtt ccacgcggtt aatcgcctca
    14341 acgggttgcg ccagtttgct gttgatctca ccgcttgccg gaaacgccgc catccggtcg
    14401 cgcaccagtt cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac
    14461 gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttcg
    14521 ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc
    14581 gacattaccg gcgtgccgcc tgcagcagtc accacatcaa tagtgttcca agagagacgc
    14641 ggatcatgga tgatcttcgc gccgggattt ttttcgagga acgcttctgc cagcaggccg
    14701 acaatgtagt agccctcgat aaactgccct ttttcgtcaa acagaaaaca gcggtcaaaa
    14761 tcgccatcaa aggcaatgcc catatccgcg ccgtgtttga tgaccgcatt gcgggtgtcg
    14821 tcgcgacatt ccggcagcag cgggttagga ataccgttgg ggaaattgcc gtccggtgtg
    14881 ttgtgtactt tgattaattc cacgggcgcg ccgagggctt taaagcgggc ttcaatggcg
    14941 tccaccaccg gacccgctgc gccattcccg gagttgatca ccagcttgag cggcgtgaga
    15001 tttttgacat tgatataacc gaacaggtga tcaacgtaag cgtcacgcag gttgatttgc
    15061 tgatagcgac cgcgtttggt ttcatcgacg ggaggaaagt cgttggcttc agccagacgc
    15121 tggacgtcgc gcagtccggt atcaccgctg atcgtgcgag ccccctcgcg caccagcttc
    15181 atgccgttgt aatccatcgg attatggctg gcagtaactt caatgccgcc atccacgccg
    15241 agatggaacg tggcgaaata gatctcttcg gtgccggaca taccgatatc cagcacatcg
    15301 acgcccgcat cctgtaaacc ttttgccagc gccagtttta aggtttcgct ggtgagacgg
    15361 acatcaccgc ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg
    15421 cgccaggcga tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta
    15481 aagcaggtta attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa
    15541 acggccaatt ttatttttgt cgttatttac accgcggttt cgcattcatt gcctgatgcg
    15601 acgctgccgc gtcttatcag gcctacaaaa ccgaaccgta ggtcggataa ggcgttcacg
    15661 ccgcatccga caaacagcgc ctgatgcgac gtttacaccc gtccgtagcg atccgcgaag
    15721 cgcaccacat catcctcttc gagataagag ccggagcgca cttcaattaa atcgagcgga
    15781 attttccccg ggttttccag gcagtgcgtc gcccccagcg gaatataaat ggactcgttt
    15841 tcaccaagca gtttgatatc accatcaatg gtgacttttg ccgttcccgc gaccactacc
    15901 cagtgttccg cgcggtgatg gtgcatctgt accgacaaac cctcgcccgg tttcacggtg
    15961 atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt catatttgcc ccacggacga
    16021 tacacttcgc gatgcacccg atgctcatgg cgaccatcgg ctttgatctg ctcgaccact
    16081 tttttcacat cctgtaccgc gttacggtcg gcaatcagca ccgcatcttt ggtctgcacc
    16141 actaccagat ctttcacccc gacggtggtg accaggcctg attcggcata cacatagctg
    16201 ttttcagttt tgtgattaat cacatcgccg tggcaaacgt tgccctcggc ggtgtgggcg
    16261 ctgatctccc ataatgaaga ccaggaacca acatcgctcc agcccgcatc catcggcacc
    16321 acaacagcat ctgccgtagg ttccatgacc gcgtaatcca ccgactcttc cgggcaggcg
    16381 agaaacgctt cttcatccac acgaataaaa tcgagatccg gatcgacggc gctcatcgct
    16441 ttttcgcagg catcgaggat atccgggcga tattttttca gttcttcgag atagcgtccg
    16501 gcgcggaaca ggaacatgcc gctgttccag taatattcgc cgcttgctac ataggcctgg
    16561 gcggtttcca gattcggttt ttcgacaaac tgcgccactt caaaggccac cgtatcctgc
    16621 tcacccacag acacttcacc gcgacgaata tagccataac cggtttctgg tagatccggc
    16681 acaatgccaa aggtcaccag cttgcccgct tcggcataag gcatggcatt acgcacggcg
    16741 gcacggaacg cgtcttcatc ggcaatcaca tgatccgccg ccaataccag cattaacggg
    16801 tcgctctccg ggctatgacg ttttgccgcc agcgccgcca gtgcaatggc aggtgcagtg
    16861 ttacgccctg ccggttcgag aataatgttc tcggtgagtt tgttcagttg acgcagctgt
    16921 tccgcgacaa taaagcggtg ctgctcattg caaatcacca ccgggctttc gcactccacg
    16981 ccgttcaggc ggcagatggt ggtttgcagc atggtgagat cgcctttcag gcataaaaac
    17041 tgcttggggt aaagtacgcg ggaaagcggc cataaacggc taccggagcc acctgccatc
    17101 acaactggat agagtttcga ctgcgccata attatccccg aatatcattt ataaattgac
    17161 gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc acgtgccacc gtgttatgtt
    17221 tgggcagcag gagcgcctga cgaatccccg ccaccagcgc ctcgaccgat tccggttcta
    17281 cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc cagttctgtg tgggcctctg
    17341 cggtaatcac cgcgttaccg cctaccgcca gaatattggt cagtttcgac ggcaatacgg
    17401 catctgccgc gccgcgtttt tgcaccacca gatggcaatc gcccatcttc agcagtgcag
    17461 gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt gcgcagtcca cgctgctgcg
    17521 ccattttttc cagccgcgct ttgccgccgc cctgcccgac aatggcaaaa atcagcggtt
    17581 catcgcgcag gagatcggca gcttcaataa cgttttccag cccctgcttt tcaccaatat
    17641 tgccggagta aagaatgatt tttttgttat ccggcaggcc aagctggtta cgaagggcat
    17701 caacatcggc gtctgcaaca tgctgaaaac gggcaatttc cgaccagttg gggaagaaga
    17761 tgacgttatc cgccgccacg cctttttcga tggctttatt catcatcgaa cgcgaaatcg
    17821 tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa cgccgttgcc agctgtgcca
    17881 ctttgccgcc tttgcctttt ccggcaaggc ccagccccag catggcgtcc acttcgtaat
    17941 cctgaatatg cagcacggta cgcgcaccag agagcttcgc cagcaggcgc attcccggcg
    18001 tgcaaaacag cgttggcact acgccaataa tgcgatccgg cttccagcga cgttgcgcca
    18061 tcagtggaaa gaaactgctg acggcaaaac tgccgagatg caacaagcgt ttcagggtgc
    18121 tcggctgttt tggcacgtac agcgggcagc gccacaccgt ggcggcccct tcttctcgtt
    18181 tgtagcgcca ggcggaatag ttctcaccca cctgccattg cgggtagtaa ggcggtgcgg
    18241 taatgacccg cacctcatga ccttgtgccg ccaaccattc caccatctcg ccggtgtatt
    18301 tgccgatgcc ggttaactcc ggcgagtagt taatgccgta gaccagtatt ttcataatcc
    18361 gggtactccg gcacgcttct cagcgagaaa ataggctcgg ctgttagcgt gaacatcgtt
    18421 actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg tcatgctgct catccggcag
    18481 taacagctct tcttccgcta ctctgaagcg aaaaccgagc accacatagt gagtggtgaa
    18541 atccgtgccg gagaagttat cgtcataaaa atgctgccag acaccgtaaa actggcctgc
    18601 tgttatcggc agacgcagcc ccagttccgc catcgtcagc cgctcaaatg cggcttccag
    18661 cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag taaccctgcg ccgggcggtt
    18721 ggttcttttg ccaagcagaa actcgccgcg actgttctcg acaataaagt cgagagagac
    18781 aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga cgtaaaaaca tcattccccc
    18841 cgaaagcggt cttgattctc aaggaaccac tggtaagtgc tggcaagccc cgcttccagt
    18901 gagatttcgt gataccagcc aagctgatgc aggcgcgtca catccagcag tttgcgcggc
    18961 gtaccatccg gtttgctggc atcaaaaacc acccgacctt tgtaacccac cactttggcg
    19021 atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg tgccgacgtt aatgtgcgac
    19081 agcatcggtt gggtattctc cagccagact tcatgcgcca gctccatgac atgaatgctc
    19141 gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg gtgtaccgct gccccatacc
    19201 accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc gacgcagcaa tgctggtatc
    19261 acatgggagt tactcgggtg gaagttgtca tgcggcccgt acaggttggt cggcatgaca
    19321 gtgcggtaat cgcgtccata ctggcggttg taagattcgc acagtttgat cccggcgatt
    19381 ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc cctgcaataa ctcgctttct
    19441 gccatcggct gttttgccag tttcgggtag atgcaggacg atccgagaaa catcagtttg
    19501 ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct caatcatcat gttctggtag
    19561 atgaaatccg ccggataggt gttgttggca acaatgccgc ccactttcgc cgccgccaga
    19621 tagacctggt caatgcgttc gctggcaaag aaatcatgca cggcgcggct gtccagcagg
    19681 ttcagttcat cgcgggtacg taataccagc tccacatcac cgcgctgttc gagctgccgc
    19741 ttgatggcag aaccgaccat cccacaatgg ccagcgataa aaatgcgttg tttactcatg
    19801 cttatgactc cagcgcgatc gccacgtcgt agccgtgaga tttcagcaga gagtgttttt
    19861 tcgccgcttc gaggtcatta gccaccattt cagacaccat ctctctgagg gtgatttccg
    19921 gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc gagcagcgtt tcaacttctg
    19981 ccggacggaa gtaacgcgga tcaacggcga taatcacatc acccggttta acgcccggcg
    20041 cgtcatgccc ggtgacggaa accacaatgc ccttctcttc aacacccgtg ccttcaaagc
    20101 gcagtttgat gcccagctgt gctgccgcca tttccacaaa ctgacgtacg gagtactgaa
    20161 cgccggtcgc gataacgaaa tcttccggct gttcctgttg cagcatcatc cactgcattt
    20221 ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc catattgccg aggtacaggc
    20281 acgactccag cccctgggcg atgttggcga ttgcgcgggt gattttgcgg gtaacgaagg
    20341 tttcgccgcg gcgcggggat tcatggttga agagaattcc gttacaagcg tacatgccgt
    20401 aggattcgcg gtagttaacg gtgatccagt aggcgtacag tttggcgacc gcatacggag
    20461 atcgcgggta gaacggcgtg gtttctttct gcggaatttc ctgcaccaga ccatacagtt
    20521 cagaggtgga agcctgatag aaacgagttt tcttttccag accgaggaag cggatcgcct
    20581 ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt atattctggt gactcaaaag
    20641 agaccgcaac gtggctcatt gcgcctaggt tgtacacttc atccggctgt acttcacgca
    20701 aaatgcgcgt caggttagag gtatcactca ggtcgccata atgcagatgg aatttcgggt
    20761 tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc ggtgttgaat gacgatgcgc
    20821 gacgcttaat accatgcacc tcgtaacctt tttccagcag aaactctgcc aggtaagaac
    20881 cgtcttgtcc ggttacaccg gtgatgagag cgacttttga catgtcttat tcctctgtat
    20941 ttttgaattt attcagtttc aacgcgttcg cgtatcacca ctgcgggatt cccccggcaa
    21001 accacatttg ccggaagcga tttaaaaaca ctgcttcgtg cacccacgac ggtgccgtcg
    21061 ccgattgtga cgcctggggc aacaaagaca tcggttgcca gccagcattt ctcgccaatc
    21121 acaataggcg tggcgttaat ggtgaaatgt tgacttgcat ggtcgtggct accggtgcat
    21181 aaataacttt tttgcgatat caccgaatgt gcgccaatgg ttatttcacc gagggtatat
    21241 aaattgacgt catcgccaac ccacgcgtaa tcacctaagg ttaatttcca cggataggta
    21301 atttttactg acggacgaat aactacgttt tttcctattt ttgctccgaa taaacgtaat
    21361 aaaaaagccc gccagcgata caatacttgt ggcgaccagg caaatattgt tgcctgtact
    21421 gcccaccata attgcacttt aatagcgttg ccgccccgga accctttcgg caccgagaaa
    21481 ccgcttaaat cttgcattgg ttttccttat attcagactt tgttatatac ggctttagtc
    21541 ttacctgtcg tacgttggcg taaatgccag gataattctg cccagaagcc aggtacatgt
    21601 aatatctgcc gttggacttt tttggcgtca gcac
//
LOCUS       DBBBSB010000066        21514 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.066, whole genome shotgun sequence.
ACCESSION   DBBBSB010000066 DBBBSB010000000
VERSION     DBBBSB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.066"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACIUS0_002465"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACIUS0_002465"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGB0220182.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACIUS0_002466"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACIUS0_002466"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGB0220183.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACIUS0_002467"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACIUS0_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGB0220184.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACIUS0_002468"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACIUS0_002468"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGB0220185.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACIUS0_002469"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACIUS0_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACIUS0_002470"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACIUS0_002470"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGB0220186.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACIUS0_002471"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACIUS0_002471"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGB0220187.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACIUS0_002472"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACIUS0_002472"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGB0220188.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACIUS0_002473"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACIUS0_002473"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGB0220189.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACIUS0_002474"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACIUS0_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGB0220190.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACIUS0_002475"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACIUS0_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGB0220191.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACIUS0_002476"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACIUS0_002476"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGB0220192.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACIUS0_002477"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACIUS0_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGB0220193.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACIUS0_002478"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACIUS0_002478"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGB0220194.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACIUS0_002479"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACIUS0_002479"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGB0220195.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACIUS0_002480"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACIUS0_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGB0220196.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACIUS0_002481"
     CDS             complement(18170..18565)
                     /locus_tag="ACIUS0_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGB0220197.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACIUS0_002482"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACIUS0_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0220198.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACIUS0_002483"
     CDS             19481..20074
                     /locus_tag="ACIUS0_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGB0220199.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACIUS0_002484"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACIUS0_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGB0220200.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21514
                     /locus_tag="ACIUS0_002485"
     CDS             20794..>21514
                     /locus_tag="ACIUS0_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGB0220201.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5197 a         5324 c         5668 g         5325 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aata
//
LOCUS       DBBBSB010000067        21505 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.067, whole genome shotgun sequence.
ACCESSION   DBBBSB010000067 DBBBSB010000000
VERSION     DBBBSB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.067"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="ACIUS0_002486"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="ACIUS0_002486"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGB0220202.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="ACIUS0_002487"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="ACIUS0_002487"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGB0220203.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="ACIUS0_002488"
     CDS             complement(3122..3421)
                     /locus_tag="ACIUS0_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGB0220204.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="ACIUS0_002489"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="ACIUS0_002489"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGB0220205.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /gene="yiaA"
                     /locus_tag="ACIUS0_002490"
     CDS             complement(4633..5070)
                     /gene="yiaA"
                     /locus_tag="ACIUS0_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGB0220206.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /gene="yiaB"
                     /locus_tag="ACIUS0_002491"
     CDS             complement(5116..5457)
                     /gene="yiaB"
                     /locus_tag="ACIUS0_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGB0220207.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="ACIUS0_002492"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="ACIUS0_002492"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGB0220208.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="ACIUS0_002493"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="ACIUS0_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="ACIUS0_002494"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="ACIUS0_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0220209.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="ACIUS0_002495"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="ACIUS0_002495"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGB0220210.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="ACIUS0_002496"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="ACIUS0_002496"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGB0220211.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13866
                     /gene="xylR"
                     /locus_tag="ACIUS0_002497"
                     /pseudo
     CDS             12688..13866
                     /gene="xylR"
                     /locus_tag="ACIUS0_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13974..14798)
                     /locus_tag="ACIUS0_002498"
     CDS             complement(13974..14798)
                     /locus_tag="ACIUS0_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGB0220212.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17148
                     /gene="malS"
                     /locus_tag="ACIUS0_002499"
     CDS             15118..17148
                     /gene="malS"
                     /locus_tag="ACIUS0_002499"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGB0220213.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17326..18579
                     /gene="avtA"
                     /locus_tag="ACIUS0_002500"
     CDS             17326..18579
                     /gene="avtA"
                     /locus_tag="ACIUS0_002500"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGB0220214.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18730..19203)
                     /locus_tag="ACIUS0_002501"
     CDS             complement(18730..19203)
                     /locus_tag="ACIUS0_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGB0220215.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACIUS0_002502"
     CDS             complement(19305..20153)
                     /gene="yiaJ"
                     /locus_tag="ACIUS0_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGB0220216.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAALHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="ACIUS0_002503"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="ACIUS0_002503"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGB0220217.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..>21505
                     /locus_tag="ACIUS0_002504"
     CDS             21363..>21505
                     /locus_tag="ACIUS0_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGB0220218.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5404 a         5490 c         5277 g         5334 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaaccc ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaaatgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgagc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcacccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgcccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc
    15481 ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc
    15541 gccgtacggg attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta
    15601 caacccgctg ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc
    15661 gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac
    15721 ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc
    15781 acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg
    15841 ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggt
    15901 ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg
    15961 aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat
    16021 cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg
    16081 gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa
    16141 acgctgggtg aacgctggag cgactggaaa cctgccgccg gacaaacctg gcatagcttt
    16201 aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg
    16261 atcagaacgg atatcggcga ttacgacaat cctggattcg atgatctcac tatgtcgctg
    16321 gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat
    16381 aaaaacaaaa cggatactca cgctaaagtc atcgaaggct ttacacctcg cgattactta
    16441 acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc
    16501 gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt
    16561 cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc
    16621 ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg
    16681 atgatcaatt tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg
    16741 gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc
    16801 tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta
    16861 ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct
    16921 acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt
    16981 aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc
    17041 gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt
    17101 gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt
    17161 cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg
    17221 ttgcgggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc
    17281 gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt
    17341 tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac
    17401 ggtttacgca cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa
    17461 atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat
    17521 gcactgtgta actacgacgg tccacagggg aaaacggagc tactcacact gcttgccgga
    17581 atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc
    17641 agccagagcg cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg
    17701 gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg
    17761 gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt
    17821 aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc
    17881 tcccggccga cgaatccaac aggtaatgtg attactgacg aagagttgct gaagcttgac
    17941 gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc
    18001 ccgggtatca tcttcagtga agggcgcccg ctatggaatc cgaatatcgt actgtgcatg
    18061 agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa
    18121 atcatcactg ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt
    18181 ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc
    18241 atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta
    18301 ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt
    18361 aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg
    18421 atggtgccgg ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa
    18481 tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg
    18541 gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga tgcgctacgc
    18601 ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata
    18661 aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcattg agaaacgccg
    18721 gaaaacgttt caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat
    18781 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca
    18841 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
    18901 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat
    18961 atgcccatgt tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc
    19021 acactgatga caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga
    19081 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
    19141 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
    19201 catgatcatt cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
    19261 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agcgttatgt gatcgcgccc
    19321 aggtcatccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt
    19381 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
    19441 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
    19501 agttcgtttt cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg
    19561 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
    19621 ttttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
    19681 gcggaacagt agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt
    19741 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg
    19801 gtttcaccag tggcgatgtt cagtgcctca agatgcagag cggcgatatg aatgatattc
    19861 agcgaagaca gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct
    19921 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
    19981 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
    20041 ttgctcaaaa tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc
    20101 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
    20161 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
    20221 acttgatttt tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
    20281 tatatttgaa atctagttcc gcatattgaa attttaagcc aaaaaagcga tcaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg catcggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcag gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggcatca ctgttgatga cagcgtgtgg gccaaaatcc aggcgttatg aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccaact tcaacgtcct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa gaatatttat gcgca
//
LOCUS       DBBBSB010000068        21279 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.068, whole genome shotgun sequence.
ACCESSION   DBBBSB010000068 DBBBSB010000000
VERSION     DBBBSB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21279
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.068"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..447)
                     /locus_tag="ACIUS0_002505"
     CDS             complement(<1..447)
                     /locus_tag="ACIUS0_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGB0220219.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="ACIUS0_002506"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="ACIUS0_002506"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGB0220220.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGNLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="ACIUS0_002507"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="ACIUS0_002507"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGB0220221.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="ACIUS0_002508"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="ACIUS0_002508"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGB0220222.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3517..3675
                     /gene="yoaL"
                     /locus_tag="ACIUS0_002509"
     CDS             3517..3675
                     /gene="yoaL"
                     /locus_tag="ACIUS0_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGB0220223.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="ACIUS0_002510"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="ACIUS0_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGB0220224.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="ACIUS0_002511"
     CDS             complement(5217..6815)
                     /locus_tag="ACIUS0_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGB0220225.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="ACIUS0_002512"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="ACIUS0_002512"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGB0220226.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="ACIUS0_002513"
     CDS             complement(8494..9072)
                     /locus_tag="ACIUS0_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGB0220227.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="ACIUS0_002514"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="ACIUS0_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGB0220228.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="ACIUS0_002515"
     CDS             10511..10690
                     /locus_tag="ACIUS0_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGB0220229.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10810..11109)
                     /locus_tag="ACIUS0_002516"
     CDS             complement(10810..11109)
                     /locus_tag="ACIUS0_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGB0220230.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11531..11875)
                     /locus_tag="ACIUS0_002517"
     CDS             complement(11531..11875)
                     /locus_tag="ACIUS0_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGB0220231.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12007..13917
                     /locus_tag="ACIUS0_002518"
     CDS             12007..13917
                     /locus_tag="ACIUS0_002518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGB0220232.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13975..14670
                     /gene="tsaB"
                     /locus_tag="ACIUS0_002519"
     CDS             13975..14670
                     /gene="tsaB"
                     /locus_tag="ACIUS0_002519"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGB0220233.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14710..15291
                     /gene="yeaY"
                     /locus_tag="ACIUS0_002520"
     CDS             14710..15291
                     /gene="yeaY"
                     /locus_tag="ACIUS0_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGB0220234.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15497..17182
                     /gene="fadD"
                     /locus_tag="ACIUS0_002521"
     CDS             15497..17182
                     /gene="fadD"
                     /locus_tag="ACIUS0_002521"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGB0220235.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17264..18379
                     /gene="rnd"
                     /locus_tag="ACIUS0_002522"
     CDS             17264..18379
                     /gene="rnd"
                     /locus_tag="ACIUS0_002522"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGB0220236.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="ACIUS0_002523"
     CDS             complement(18433..19398)
                     /gene="yeaX"
                     /locus_tag="ACIUS0_002523"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGB0220237.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSNGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="ACIUS0_002524"
                     /pseudo
     CDS             complement(19454..20071)
                     /gene="yeaW"
                     /locus_tag="ACIUS0_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20413..21021)
                     /locus_tag="ACIUS0_002525"
     CDS             complement(20413..21021)
                     /locus_tag="ACIUS0_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0220238.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21191..>21279
                     /locus_tag="ACIUS0_002526"
     CDS             21191..>21279
                     /locus_tag="ACIUS0_002526"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0220239.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5268 a         5272 c         5465 g         5274 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacgccggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacgacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca tacgctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggttgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga acgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaaccgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatctt tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggtgctga cgaatggttt ctgccatcac atagtgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcccgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt tgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg gtcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttact tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttaggggttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgtcttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgagt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc acagccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagtg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcatccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacgcttttt atatgtgatt aaaagcgtcc tgacccattt atgctgatat
    10801 aaatttaact cagaccagtg cttttagttc ttcctgcata tatgttgaaa agtattcagg
    10861 atttttaatc acagagcgtt cacctgtctc catttttttt gcccatccaa ccattttttc
    10921 cgtgaaccca gggttccagc cttcctggtc agctcgcgcc gtaatatcag cggcactcgc
    10981 gggtactccc agttctttta aatatttaaa gattcccttc gccgtgcttt cattcatcga
    11041 acttggtgtt ggcgctgata catccatggc accaatgaaa tccagggctt tatctattac
    11101 agcaggcata ttcttatcct taaaattaac catgttatcg ataaggctat atatgacctt
    11161 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
    11221 caggtgtttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
    11281 gtcagcccgc acgctataac cagattgttc agctaagaaa gaaaaccaga gttgctaatc
    11341 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagtttta tagataaaat
    11401 tgaaattata aatataaata atgaattagc agaagcatca acagtaatat cttattctct
    11461 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
    11521 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
    11581 aacccgcttg taccgtgcag cgcaccggcg catgacccgc gacaacccag gcatcccacg
    11641 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
    11701 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
    11761 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
    11821 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
    11881 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggttgccgtc
    11941 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
    12001 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
    12061 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
    12121 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
    12181 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
    12241 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
    12301 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
    12361 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
    12421 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
    12481 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
    12541 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
    12601 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgagtt gatccctgaa
    12661 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
    12721 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
    12781 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
    12841 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
    12901 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacacctt ggaactttgt
    12961 tatgatgtgg cgaaactgtc actggggcgt tccgccttgc tggatgcggc atttgagcgc
    13021 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
    13081 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
    13141 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
    13201 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
    13261 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
    13321 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
    13381 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
    13441 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
    13501 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
    13561 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt aattatcgac
    13621 aaattgccgt ttacctcgcc ggatgatcca ctgttaaaag cgcgcatgga agattgtcgt
    13681 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
    13741 cagggggtag gtcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
    13801 tatcggctgg tgatgcgtcc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
    13861 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
    13921 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
    13981 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
    14041 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
    14101 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
    14161 cgcggccccg gtagctttac cggcgtgcgc attggtattg gcatcgcgca agggctggcg
    14221 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
    14281 tggcgcaaaa acggcgcaac acgcgtgctg tcagccattg atgcgcgaat gggcgaagtt
    14341 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
    14401 gtactcaaac ccgaactcgt tcatgaacga atgcaacagc tttccggcga atgggtgacg
    14461 gtagggacgg gctggcaagc ctggccggat ctcggtaaag agagcgggct ggttttgcgc
    14521 gatggcgaag tgttactccc cgctgcagaa gatatgctgc cgattgcttg tcagatgttt
    14581 gccgagggta aaacggtggc ggtggaacat gccgagccgg tttatttgcg taacaacgtc
    14641 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
    14701 agtcgcatca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
    14761 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag taccacgccg
    14821 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
    14881 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
    14941 accgtaccgc tggacagcgg agctaggccg acgctgggag aaccttctcg cggtcgcatt
    15001 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
    15061 gtcgggccaa tcaccggtgc ggttgacggc aaaatcggca atacgcccta taaatttatg
    15121 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
    15181 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
    15241 ggctggtata atcccggccc cgcgagagta caaacagttg taaccgaata attgcttgtt
    15301 tttaaagaat gcagaaacag cggctggtcc gctgtttctg cattcttacg gtaaagataa
    15361 aaataaatag tgacgcgctt cgcaaccttt tcgttgggta attatcaagc tggtatgatg
    15421 agttaatatt atgttaacgg cgtgtatatc atttggggtt gcgatgacga cgaacacgca
    15481 ttttagaggt gaagaattga agaaggtttg gcttaaccgt tatcccgcgg acgttccgac
    15541 ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt cggtcgcgcg
    15601 ctacgccgat cagcctgcgt ttgtgaatat gggggaggtg atgaccttcc gcaagctgga
    15661 agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga agaaaggcga
    15721 tcgcgttgcg ttgatgatgc ctaacttatt gcaatatccg gtggcgctat ttggcatttt
    15781 gcgtgccggg ataatcgtcg taaacgttaa cccgttgtat accccgcgtg agcttgagca
    15841 tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg cccacactct
    15901 ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta tgggcgatca
    15961 gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca agcgcttggt
    16021 gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata acggctaccg
    16081 gatgcagtac gtcaaacccg aactggtgcc ggaagattta gccttcctgc aatacaccgg
    16141 cggcaccaca ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc tggcgaacct
    16201 ggaacaggtc aacgccacct atgggccgtt gctgcatcca ggtaaagaac tggtagtaac
    16261 ggcactgccg ctgtatcaca tttttgccct gaccattaac tgcctgctgt ttatcgaact
    16321 gggtgggcag aacctgctta tcactaaccc gcgtgatatt ccagggttgg taaaagagtt
    16381 agcgaaatat ccgtttaccg ctatcactgg cgttaacacc ttgttcaatg cgttgctgaa
    16441 caataaagag ttccagcagc tggatttctc cagtctgcat ctttccgcag gcggtgggat
    16501 gcctgttcag caagtggtgg cagaacgctg ggtgaaactg accggacagt atctgctgga
    16561 aggttatggc ctgaccgagt gcgcgccgct ggtgagcgtt aacccgtatg atattgatta
    16621 tcatagcggt agcatcggtt tgccggtgcc atcgacggaa gccaaactgg tggatgacga
    16681 cgacaacgaa gtaccaccgg gtcaaccggg tgagctttgt gtcaaaggac cgcaggtgat
    16741 gctgggttac tggcagcgtc cggatgctac agatgagatc atcaaaaatg gctggttaca
    16801 caccggcgac atcgcggtga tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa
    16861 agacatgatt ttggtttccg gttttaacgt ttatcccaac gagattgaag atgtcgtcat
    16921 gcagcatcct ggcgtacagg aagtcgcggc tgttggcgta ccttccggct ccagtggtga
    16981 agcggtgaaa atcttcgtag tgaaaaaaga tccatcgctt accgaagagt cactggtgac
    17041 tttttgccgc cgtcagctca cgggatacaa agtaccgaag ctggtggagt ttcgtgatga
    17101 gttaccgaaa tctaacgtcg gaaaaatttt gcgacgagaa ttacgtgacg aagcgcgcgg
    17161 caaagtggac aataaagcct gagcgttaag tcagtcgtca gacgccggtt aatccggcgt
    17221 tttttttgac gcccactaaa gagaaaacaa tttgaattac caaatgatta ccacggacga
    17281 tgcgctggct tctttgtgtg aagccgtccg tgtctttccg gcgatagccc tggatactga
    17341 atttgttcgt acgcgcactt attacccgca gctggggttg attcaacttt tcgatggcga
    17401 gcatctggcg ctaattgatc ctctcgggat caccgactgg tcaccgctga aagcgatcct
    17461 gcgcgatccg tccatcacaa aatttctcca tgcaggcagt gaagatctgg aagtgttcct
    17521 caatgtcttt ggcgaattac cacaaccctt gattgacacg caaatccttg ctgccttctg
    17581 cggacgcccg atgtcatggg gtttcgcttc catggtggaa gagtattccg gcgttacgct
    17641 ggacaagagt gaatcgcgca ccgactggct ggccagaccg ctgaccgaac gtcagtgtga
    17701 atacgcagcg gcggatgtct ggtatctgtt accgatcacc gccaagctta tggtagaaac
    17761 ggaggcctcc ggctggctac ctgcggcgct ggatgaatgc cgcctgatgc aaatgcgtcg
    17821 tcaggaagtc gttgcgccgg aagatgcctg gcgtgatatc accaatgcct ggcagttacg
    17881 cacacgccaa ctggcctgtc tgcaactgtt agccgactgg cgactgcgca aggcgcgaga
    17941 gcgtgatctg gcggtgaact ttgtcgtgcg tgaagagcat ttgtggtcgg tcgcgcgtta
    18001 tatgccggga agtttaggcg aactggacag cctgggttta tccggtagcg aaatccgctt
    18061 tcacggtaaa acgctgctgg cgctggtgga aaaagcgcag acattgccgg aagatgcctt
    18121 accgcagccg atgcttaacc tgatggacat gcctggttat cgtaaagcgt ttaaagcgat
    18181 taagtcgctg attactgacg tgagcgaaac gcataagatc agcgccgaat tgctggcatc
    18241 gcgtcggcaa atcaaccaac tgctgaactg gcactggaaa ctgaaaccgc agaacaattt
    18301 gccggagctg atttccggct ggcgtggtga gctgatggcg gaagcattac acaatttatt
    18361 gcaggaatat ccgcagtaaa atcttccgaa gccggactgg gcgcgctcag cgccacatcc
    18421 ggctttggca aactacaaat ccaacaccag gcgtttaccc ttcgcacgcg aacaacagat
    18481 caacatactt tgctggctgg cacgctcttc atcgctaaaa tactgatccc gatggtcagc
    18541 ttcaccttcc agtattgccg tttcgcaggt tccgcatacc ccttcacgac ataaacattc
    18601 cactttcgcg gctttattat tttcaataac ctgcaaaata gtcatctctt ccggcaccac
    18661 aaactctttt ccggaacggg caagcaccag ggtaaatgca tcgccggttt tgtcttcgat
    18721 agcaaattgc tcaaagtgca gtgtatcggc ggcgatgtcc agacgcgcag cttcacttct
    18781 taccgcttca attagcgcct cggggccaca ggtataaacg tgtgtgccag gttcgatatc
    18841 cgccaataat cgcgctaatt ccagtcgtgt tccggttgat gaatgatgca aatggacttt
    18901 ctcagcctgc ggatgctgga ctagctcagc acgaaatgcg caactttctg gatttcgcga
    18961 gcagtaatgt agctgccagt cgatgtcgct gtgttgcaac tctgccatgt gcgacaggaa
    19021 aggggtgata ccaataccgc ccacgataaa cagatgcttt ctggctgagg gaattagcgc
    19081 aaacaggtta ttaggcgttg agatcgttaa ccgatcgccc acttttacct gctgatgcaa
    19141 aaagcgggaa ccgccgcgcg agttttcttc cagtcgaacg gcaatctgat aactagaggt
    19201 gttatgcggt gaactcagta gtgaatacgc attgctgtac tggttatcac cattgctcat
    19261 ctgcactagg atgtgacttc ctccggtgaa cgcaggcagc ggtttgccat cggttgccac
    19321 cagcgtgaag cgtttcacct gttcggtaag gggttcaacc tggctcacct gtacttcaaa
    19381 catttgatag tctgacattg ctgtactcca tcaaaaatca gcccagcggc ggaaaatacc
    19441 gccgccgatg tcattagtcc ttaaacacct gcgccagcag attatggaaa tgggcgatac
    19501 catgttcgga aatgccacta ccgctactgt cggccatgat gcgcccctga ccacgatagc
    19561 cacgcgattt cagccctttc tgtacgcttt caaccagacg taaatcttcc ggatggaaca
    19621 catcgcgata ccactcaatc agcgattttt gctcgtcggt taactcttca ttggtgaagt
    19681 aaatatcgta gttttgcagg gtagtttcag aatccaccgg gaattcataa atgaccgtca
    19741 tcatcccttt gatcggggtg acgttcagca tcgtgcacgg ccacagccag aaaccgtgga
    19801 atgccgcatc cgtaccctct tcaaatttaa acgactgttc ggacggtttg gcgaaaccgt
    19861 attgcagcgt ccagttaccg tgcatggtgt gccagtaacg atcaacctgt acggagtcgg
    19921 agaaacctag atgcgccgga ccacagtgat agcactcgag atagttatcg acaatgttct
    19981 tccagttggc aggcgtgcgg gtggtaaagc gggccgctag tttcagattg tggacttccg
    20041 ggcaggcttc cagcactttc gcgcccagac cggtgtattg attcacttga agtacgaaaa
    20101 aaaaccggga ggacattgga ttattcgggt tctgatggga ttagatttgg tggggcttgc
    20161 aagcctgtag cgcaaatttt agtcgttaat caatgaaacg cgaaagatag taaaaaattg
    20221 cttttgtttc attgaaaata cgaaaaacaa aaacactgca aatcatttca ataacagctt
    20281 caaaaaacgt tcaaaaccga taacaaccag gctgtaacca aatgactcat atcacaaatc
    20341 agctcatgcc gtttaggtat gttacatgtg tgattatgtg aggtgaagta tgttttagct
    20401 ggttcatggt tgttatacgg ctttttttac atcttgtggt tcttgtgaag gagctacaag
    20461 accttcctgt tcataaatac catactttga caaaatcttt ttacgagtat ctgttggtaa
    20521 tgattcaagc attttggctg tttgtaaggt ttgctgcgaa ataccttgca aatcagcctg
    20581 gatggaatca tcggaagaaa aaatctcagg cataagagct tcaaatcctt tctttctgaa
    20641 caactcaatc gcagaatgca gttccttgtc ctctaacaat tcgacaattg cgatcagttt
    20701 agttttcaat gtgctgcggt caggttgaga atttttattc tcttgattat ttttatgctc
    20761 aaccattggc atgctctgac tttggataaa ccactctatt ggacgccctg ttgcgtaggc
    20821 tatttgtgct aatcgatcta aagacgggta tgtcttgcca gacaggtagt ttctgataac
    20881 cccatcagaa agtccgcact tagctgcaaa cgcccggctt gattctccat tcattgcctc
    20941 ttcaaggcgt tccttgaaac gcattatttc cgggggcata aaaatgcgct ctgcattttt
    21001 gctttctttt tcacctgtca tgcccaatct ctctgttata taaagaattt tacagaattc
    21061 tttgatttgc actggttaaa ataaaaatgc gcatagcttt tgaattgagc atttttctgc
    21121 gctatcgttt ttcccgatag cggtagccgg tatcagttac cgaaaacgtt aagagagtaa
    21181 ggatcgcaga atgtcagcaa aaaaccaaga ctggcacgca gagcagataa aggctgctgt
    21241 tcgtatgaag gggaagacac tatctcaatt atccagaga
//
LOCUS       DBBBSB010000069        21256 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.069, whole genome shotgun sequence.
ACCESSION   DBBBSB010000069 DBBBSB010000000
VERSION     DBBBSB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.069"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..794
                     /locus_tag="ACIUS0_002527"
                     /pseudo
     CDS             1..794
                     /locus_tag="ACIUS0_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACIUS0_002528"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="ACIUS0_002528"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGB0220240.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACIUS0_002529"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="ACIUS0_002529"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGB0220241.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACIUS0_002530"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="ACIUS0_002530"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGB0220242.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACIUS0_002531"
     CDS             complement(4992..7226)
                     /gene="relA"
                     /locus_tag="ACIUS0_002531"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGB0220243.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACIUS0_002532"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="ACIUS0_002532"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGB0220244.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="ACIUS0_002533"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="ACIUS0_002533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGB0220245.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACIUS0_002534"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="ACIUS0_002534"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGB0220246.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="ACIUS0_002535"
     CDS             complement(12981..14321)
                     /locus_tag="ACIUS0_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGB0220247.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACIUS0_002536"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="ACIUS0_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGB0220248.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="ACIUS0_002537"
     CDS             complement(16109..16558)
                     /locus_tag="ACIUS0_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGB0220249.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACIUS0_002538"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="ACIUS0_002538"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGB0220250.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="ACIUS0_002539"
     CDS             complement(17358..17687)
                     /locus_tag="ACIUS0_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGB0220251.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACIUS0_002540"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="ACIUS0_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGB0220252.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="ACIUS0_002541"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="ACIUS0_002541"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGB0220253.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="ACIUS0_002542"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="ACIUS0_002542"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGB0220254.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5228 a         5671 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccatcat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DBBBSB010000070        20780 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.070, whole genome shotgun sequence.
ACCESSION   DBBBSB010000070 DBBBSB010000000
VERSION     DBBBSB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.070"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..579
                     /locus_tag="ACIUS0_002543"
     CDS             <1..579
                     /locus_tag="ACIUS0_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGB0220255.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACIUS0_002544"
     CDS             597..1235
                     /locus_tag="ACIUS0_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0220256.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACIUS0_002545"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACIUS0_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGB0220257.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACIUS0_002546"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACIUS0_002546"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGB0220258.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACIUS0_002547"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACIUS0_002547"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGB0220259.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACIUS0_002548"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACIUS0_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGB0220260.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACIUS0_002549"
     CDS             complement(5552..6613)
                     /locus_tag="ACIUS0_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGB0220261.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACIUS0_002550"
     CDS             complement(6610..8007)
                     /locus_tag="ACIUS0_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGB0220262.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACIUS0_002551"
     CDS             8163..9161
                     /locus_tag="ACIUS0_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGB0220263.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACIUS0_002552"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACIUS0_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACIUS0_002553"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACIUS0_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACIUS0_002554"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACIUS0_002554"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGB0220264.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACIUS0_002555"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACIUS0_002555"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HGB0220265.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15286)
                     /locus_tag="ACIUS0_002556"
                     /pseudo
     CDS             complement(14232..15286)
                     /locus_tag="ACIUS0_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(15296..16071)
                     /locus_tag="ACIUS0_002557"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="ACIUS0_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(16089..17141)
                     /locus_tag="ACIUS0_002558"
     CDS             complement(16089..17141)
                     /locus_tag="ACIUS0_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGB0220266.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="ACIUS0_002559"
     CDS             complement(17172..18014)
                     /locus_tag="ACIUS0_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGB0220267.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="ACIUS0_002560"
     CDS             complement(18001..18882)
                     /locus_tag="ACIUS0_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGB0220268.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="ACIUS0_002561"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="ACIUS0_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACIUS0_002562"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="ACIUS0_002562"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGB0220269.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5669 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg acccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DBBBSB010000071        20712 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.071, whole genome shotgun sequence.
ACCESSION   DBBBSB010000071 DBBBSB010000000
VERSION     DBBBSB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.071"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACIUS0_002563"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACIUS0_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGB0220270.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACIUS0_002564"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACIUS0_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGB0220271.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACIUS0_002565"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACIUS0_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGB0220272.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACIUS0_002566"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACIUS0_002566"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGB0220273.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACIUS0_002567"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACIUS0_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGB0220274.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACIUS0_002568"
     CDS             complement(8217..9110)
                     /locus_tag="ACIUS0_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGB0220275.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACIUS0_002569"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACIUS0_002569"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGB0220276.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACIUS0_002570"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACIUS0_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGB0220277.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACIUS0_002571"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACIUS0_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGB0220278.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACIUS0_002572"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACIUS0_002572"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGB0220279.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACIUS0_002573"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACIUS0_002573"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGB0220280.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACIUS0_002574"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACIUS0_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACIUS0_002575"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACIUS0_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGB0220281.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACIUS0_002576"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACIUS0_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGB0220282.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACIUS0_002577"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACIUS0_002577"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGB0220283.1"
                     /translation="MMAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACIUS0_002578"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACIUS0_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGB0220284.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5726 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagaggct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc atcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DBBBSB010000072        20692 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.072, whole genome shotgun sequence.
ACCESSION   DBBBSB010000072 DBBBSB010000000
VERSION     DBBBSB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.072"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            81..350
                     /locus_tag="ACIUS0_002579"
     CDS             81..350
                     /locus_tag="ACIUS0_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGB0220285.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACIUS0_002580"
     CDS             344..709
                     /locus_tag="ACIUS0_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGB0220286.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACIUS0_002581"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACIUS0_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGB0220287.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACIUS0_002582"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACIUS0_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGB0220288.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACIUS0_002583"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACIUS0_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGB0220289.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACIUS0_002584"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACIUS0_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGB0220290.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACIUS0_002585"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACIUS0_002585"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGB0220291.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACIUS0_002586"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACIUS0_002586"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGB0220292.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACIUS0_002587"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACIUS0_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACIUS0_002588"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACIUS0_002588"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGB0220293.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACIUS0_002589"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACIUS0_002589"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGB0220294.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACIUS0_002590"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACIUS0_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGB0220295.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACIUS0_002591"
     CDS             complement(16429..17187)
                     /locus_tag="ACIUS0_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGB0220296.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACIUS0_002592"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACIUS0_002592"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGB0220297.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACIUS0_002593"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACIUS0_002593"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGB0220298.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACIUS0_002594"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACIUS0_002594"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGB0220299.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACIUS0_002595"
     CDS             complement(20306..20587)
                     /locus_tag="ACIUS0_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220300.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4889 a         5694 c         5217 g         4892 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg gc
//
LOCUS       DBBBSB010000073        20523 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.073, whole genome shotgun sequence.
ACCESSION   DBBBSB010000073 DBBBSB010000000
VERSION     DBBBSB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.073"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="ACIUS0_002596"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="ACIUS0_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGB0220301.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="ACIUS0_002597"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="ACIUS0_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HGB0220302.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="ACIUS0_002598"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="ACIUS0_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGB0220303.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACIUS0_002599"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="ACIUS0_002599"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGB0220304.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACIUS0_002600"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="ACIUS0_002600"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGB0220305.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="ACIUS0_002601"
     CDS             4451..4816
                     /locus_tag="ACIUS0_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGB0220306.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="ACIUS0_002602"
     CDS             4966..5484
                     /locus_tag="ACIUS0_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGB0220307.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="ACIUS0_002603"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="ACIUS0_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="ACIUS0_002604"
     CDS             6715..7197
                     /locus_tag="ACIUS0_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGB0220308.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACIUS0_002605"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="ACIUS0_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGB0220309.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACIUS0_002606"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="ACIUS0_002606"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGB0220310.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTRL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACIUS0_002607"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="ACIUS0_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGB0220311.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACIUS0_002608"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="ACIUS0_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGB0220312.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACIUS0_002609"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="ACIUS0_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGB0220313.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="ACIUS0_002610"
     CDS             10941..11732
                     /locus_tag="ACIUS0_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGB0220314.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="ACIUS0_002611"
     CDS             11753..13039
                     /locus_tag="ACIUS0_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGB0220315.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACIUS0_002612"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="ACIUS0_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGB0220316.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="ACIUS0_002613"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="ACIUS0_002613"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGB0220317.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="ACIUS0_002614"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="ACIUS0_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGB0220318.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="ACIUS0_002615"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="ACIUS0_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGB0220319.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="ACIUS0_002616"
     CDS             complement(16987..17277)
                     /locus_tag="ACIUS0_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGB0220320.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACIUS0_002617"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="ACIUS0_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGB0220321.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="ACIUS0_002618"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="ACIUS0_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGB0220322.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="ACIUS0_002619"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="ACIUS0_002619"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="ACIUS0_002620"
     CDS             19139..20380
                     /locus_tag="ACIUS0_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGB0220323.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5299 a         5359 c         4815 g         5050 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacatcccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc ctggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DBBBSB010000074        20290 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.074, whole genome shotgun sequence.
ACCESSION   DBBBSB010000074 DBBBSB010000000
VERSION     DBBBSB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20290
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.074"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACIUS0_002621"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACIUS0_002621"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGB0220324.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACIUS0_002622"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACIUS0_002622"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGB0220325.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="ACIUS0_002623"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="ACIUS0_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="ACIUS0_002624"
     CDS             complement(3184..4389)
                     /locus_tag="ACIUS0_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220326.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="ACIUS0_002625"
     CDS             complement(4489..4845)
                     /locus_tag="ACIUS0_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGB0220327.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="ACIUS0_002626"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACIUS0_002626"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACIUS0_002627"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACIUS0_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGB0220328.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="ACIUS0_002628"
     CDS             6775..9831
                     /locus_tag="ACIUS0_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGB0220329.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKALHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="ACIUS0_002629"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="ACIUS0_002629"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGB0220330.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="ACIUS0_002630"
     CDS             10338..10526
                     /locus_tag="ACIUS0_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGB0220331.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="ACIUS0_002631"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="ACIUS0_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGB0220332.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="ACIUS0_002632"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="ACIUS0_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGB0220333.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="ACIUS0_002633"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="ACIUS0_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGB0220334.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="ACIUS0_002634"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="ACIUS0_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGB0220335.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="ACIUS0_002635"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="ACIUS0_002635"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGB0220336.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="ACIUS0_002636"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="ACIUS0_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGB0220337.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="ACIUS0_002637"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="ACIUS0_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGB0220338.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACIUS0_002638"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACIUS0_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGB0220339.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACIUS0_002639"
     CDS             complement(18349..19761)
                     /gene="pykF"
                     /locus_tag="ACIUS0_002639"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGB0220340.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(19769..19963)
                     /locus_tag="ACIUS0_002640"
     CDS             complement(19769..19963)
                     /locus_tag="ACIUS0_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220341.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         5317 a         4863 c         5176 g         4934 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agccctgcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcagtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa acaggatgct tccatcggat tcatcttaaa taaagtgaga
    18241 ttatatagtg aaatccgatt tgatttttta ttgcttctgg tcatcgatta aataaaaaaa
    18301 gcgcccatca gggcgcttcg atatacaaat taattcacaa aagcaatatt acaggacgtg
    18361 aacagatgcg gtgttagtag tgccgctcgg taccagtgca ccagaaacca taactacgac
    18421 gtcacctttg tgtgccagac cgctctgcag agccagttct ttacccagac ggtagaaatc
    18481 atcagtagaa gtgatctctt taaccagctg cggcacaacg cctttgctca gtaccaactg
    18541 atgagccgtt ttttcgttgg tggtcagtgc caggatggtg gcatccggga agtatttacg
    18601 tactgcgcga gcagatttac cgccctgggt agcaaccacg atcagcggag catccagttt
    18661 ttcagcagtt tcaacggcac cacggcatac cgcttcggtg atgcgcagtt tacggttgtc
    18721 attgttgaac tcgagacggc tgttcatcac gcggtcggta cgttcgcaga tggtcgccat
    18781 gatagaaacc gcttccagcg ggtatttacc ttttgcggat tcaccagaca gcatcactgc
    18841 gtcagtaccg tcgaggatgg cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg
    18901 gtttttgatc atggaatcca gcatctgggt cgcagtgata acgactttac gtgcacggat
    18961 acatttttcg atcatcatct tctgggcgaa gataacttct tctaccggga tttctacacc
    19021 caggtcgcca cgcgcaacca tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt
    19081 gaggccttcc tggttttcga ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt
    19141 caggtgctca cggatttcga taacgtcaga acgcttacga ataaaggaag cagcaacaaa
    19201 gtctacgcct ttttcgcaac caaagatcag gtcctgtttg tctttttcag ccagtgctgg
    19261 cagagcaatg gaaacgccag gcaggttcac acctttgttt tcgcccaggt caccgttgtt
    19321 cagcacttta cagataactt tgttaccttc aatggcggta acttccatac cgatcagacc
    19381 atcgtcaacc agtacggtgt tgccaacaga caggtcagta gtgaaacctt catacgttac
    19441 cgcaaccatt tcgctgttgc cgataacaga tttatcagtg gtgaaagtaa aggtctgacc
    19501 agctttcaga gaaacatcgt taccgccttc cagtttcatg gtgcggattt ccggaccttt
    19561 ggtatcaagc aggatagcgg cggttttacc agttttgctc atcacgttgc gcagattctg
    19621 aatgcgctga ccgtgttctg catagtcacc atgagagaag ttcagacgca taacgttcat
    19681 gccagcgtcc agcattttag ctaacatctc ttcagattcg gttttcggtc cgatggtgca
    19741 aacaattttg gtctttttca tgacagtctt agtctttaag ttgagaagga tgggagaaac
    19801 ttgctttctg ggcgcactgc cggaaagcga atccggtatt acgaaagtgg tggtgcctcg
    19861 ctctaaggat aggtgacatc gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc
    19921 aacgtgagtg tctgtttttg agtcgttgag ttctacagtc caaagtgctg aaaccattca
    19981 agagtcaatt ggcgcgcatt atacgctgaa attaatccaa aaggaaaaca aaaacagcgt
    20041 ttcaaaaaat atacaacttg ttagacaata aattgttatc aaagatttaa cgttccaggg
    20101 aaaaggttac gcaaaaactt cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg
    20161 aggataagtg aaagttcatc cggtgtataa atatatcatc gctcaattgc tacagttaaa
    20221 ataatttatc gcgttatttg tctgattttg tttgattttt gtttaattgg ttagattgat
    20281 atatgctgat
//
LOCUS       DBBBSB010000075        20192 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.075, whole genome shotgun sequence.
ACCESSION   DBBBSB010000075 DBBBSB010000000
VERSION     DBBBSB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20192
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.075"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACIUS0_002641"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACIUS0_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGB0220342.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACIUS0_002642"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACIUS0_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGB0220343.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACIUS0_002643"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACIUS0_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGB0220344.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACIUS0_002644"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACIUS0_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGB0220345.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACIUS0_002645"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACIUS0_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACIUS0_002646"
     CDS             2795..3100
                     /locus_tag="ACIUS0_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGB0220346.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACIUS0_002647"
     CDS             3103..3507
                     /locus_tag="ACIUS0_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGB0220347.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACIUS0_002648"
     CDS             3497..3796
                     /locus_tag="ACIUS0_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGB0220348.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACIUS0_002649"
     CDS             3982..4278
                     /locus_tag="ACIUS0_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGB0220349.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACIUS0_002650"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACIUS0_002650"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGB0220350.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACIUS0_002651"
     CDS             5627..5995
                     /locus_tag="ACIUS0_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGB0220351.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACIUS0_002652"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACIUS0_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGB0220352.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="ACIUS0_002653"
     CDS             7203..7904
                     /locus_tag="ACIUS0_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGB0220353.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /gene="yhaL"
                     /locus_tag="ACIUS0_002654"
     CDS             7927..8091
                     /gene="yhaL"
                     /locus_tag="ACIUS0_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGB0220354.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACIUS0_002655"
     CDS             complement(8303..9613)
                     /gene="cyuA"
                     /locus_tag="ACIUS0_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGB0220355.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="ACIUS0_002656"
     CDS             complement(9641..10972)
                     /locus_tag="ACIUS0_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGB0220356.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACIUS0_002657"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="ACIUS0_002657"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGB0220357.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="ACIUS0_002658"
     CDS             complement(12683..13072)
                     /locus_tag="ACIUS0_002658"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGB0220358.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACIUS0_002659"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="ACIUS0_002659"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGB0220359.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACIUS0_002660"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="ACIUS0_002660"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGB0220360.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACIUS0_002661"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="ACIUS0_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGB0220361.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACIUS0_002662"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="ACIUS0_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGB0220362.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACIUS0_002663"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="ACIUS0_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGB0220363.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5236 a         5282 c         4817 g         4857 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcaacgctt taattttctt cataaagcgt
    13621 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca ta
//
LOCUS       DBBBSB010000076        20091 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.076, whole genome shotgun sequence.
ACCESSION   DBBBSB010000076 DBBBSB010000000
VERSION     DBBBSB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.076"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(435..641)
                     /locus_tag="ACIUS0_002664"
     CDS             complement(435..641)
                     /locus_tag="ACIUS0_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGB0220364.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACIUS0_002665"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACIUS0_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGB0220365.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACIUS0_002666"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACIUS0_002666"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGB0220366.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACIUS0_002667"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACIUS0_002667"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACIUS0_002668"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACIUS0_002668"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGB0220367.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="ACIUS0_002669"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="ACIUS0_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="ACIUS0_002670"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="ACIUS0_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8884..9018
                     /locus_tag="ACIUS0_002671"
     CDS             8884..9018
                     /locus_tag="ACIUS0_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGB0220368.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            8997..9101
                     /gene="speFL"
                     /locus_tag="ACIUS0_002672"
     CDS             8997..9101
                     /gene="speFL"
                     /locus_tag="ACIUS0_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGB0220369.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="ACIUS0_002673"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="ACIUS0_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="ACIUS0_002674"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="ACIUS0_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGB0220370.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACIUS0_002675"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="ACIUS0_002675"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGB0220371.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACIUS0_002676"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="ACIUS0_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGB0220372.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="ACIUS0_002677"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="ACIUS0_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGB0220373.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="ACIUS0_002678"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="ACIUS0_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGB0220374.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="ACIUS0_002679"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="ACIUS0_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGB0220375.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="ACIUS0_002680"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="ACIUS0_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGB0220376.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="ACIUS0_002681"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="ACIUS0_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGB0220377.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACIUS0_002682"
     CDS             complement(18063..18389)
                     /gene="chiQ"
                     /locus_tag="ACIUS0_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGB0220378.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACIUS0_002683"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="ACIUS0_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGB0220379.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4660 a         5030 c         5316 g         5085 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagttcg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc tgtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa g
//
LOCUS       DBBBSB010000077        19698 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.077, whole genome shotgun sequence.
ACCESSION   DBBBSB010000077 DBBBSB010000000
VERSION     DBBBSB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.077"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACIUS0_002684"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACIUS0_002684"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGB0220380.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACIUS0_002685"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACIUS0_002685"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGB0220381.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACIUS0_002686"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACIUS0_002686"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGB0220382.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACIUS0_002687"
     CDS             6038..6658
                     /locus_tag="ACIUS0_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGB0220383.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACIUS0_002688"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACIUS0_002688"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGB0220384.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACIUS0_002689"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACIUS0_002689"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGB0220385.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACIUS0_002690"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACIUS0_002690"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGB0220386.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACIUS0_002691"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACIUS0_002691"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGB0220387.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACIUS0_002692"
     CDS             complement(10068..11069)
                     /locus_tag="ACIUS0_002692"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0220388.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACIUS0_002693"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACIUS0_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACIUS0_002694"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACIUS0_002694"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGB0220389.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACIUS0_002695"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACIUS0_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGB0220390.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACIUS0_002696"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACIUS0_002696"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGB0220391.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACIUS0_002697"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACIUS0_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGB0220392.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACIUS0_002698"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACIUS0_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGB0220393.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACIUS0_002699"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACIUS0_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGB0220394.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5030 g         4479 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gactccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DBBBSB010000078        19061 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.078, whole genome shotgun sequence.
ACCESSION   DBBBSB010000078 DBBBSB010000000
VERSION     DBBBSB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.078"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1025)
                     /locus_tag="ACIUS0_002700"
     CDS             complement(<1..1025)
                     /locus_tag="ACIUS0_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220395.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACIUS0_002701"
     CDS             complement(1127..2107)
                     /locus_tag="ACIUS0_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGB0220396.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACIUS0_002702"
     CDS             complement(2115..2825)
                     /locus_tag="ACIUS0_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGB0220397.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACIUS0_002703"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACIUS0_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGB0220398.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACIUS0_002704"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACIUS0_002704"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGB0220399.1"
                     /translation="MSAEHVLTMLNEHEVTFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACIUS0_002705"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACIUS0_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGB0220400.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACIUS0_002706"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACIUS0_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGB0220401.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACIUS0_002707"
     CDS             9402..9512
                     /locus_tag="ACIUS0_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGB0220402.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACIUS0_002708"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACIUS0_002708"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGB0220403.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACIUS0_002709"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACIUS0_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGB0220404.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACIUS0_002710"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACIUS0_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACIUS0_002711"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACIUS0_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGB0220405.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACIUS0_002712"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACIUS0_002712"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGB0220406.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGHRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACIUS0_002713"
     CDS             16558..17490
                     /locus_tag="ACIUS0_002713"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGB0220407.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACIUS0_002714"
     CDS             complement(17531..18961)
                     /locus_tag="ACIUS0_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGB0220408.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4662 a         4806 c         4564 g         5029 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgacgtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgatgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat t
//
LOCUS       DBBBSB010000079        18914 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.079, whole genome shotgun sequence.
ACCESSION   DBBBSB010000079 DBBBSB010000000
VERSION     DBBBSB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.079"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACIUS0_002715"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACIUS0_002715"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGB0220409.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACIUS0_002716"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACIUS0_002716"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGB0220410.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4117..4359
                     /gene="yjbE"
                     /locus_tag="ACIUS0_002717"
     CDS             4117..4359
                     /gene="yjbE"
                     /locus_tag="ACIUS0_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGB0220411.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4473..5111
                     /locus_tag="ACIUS0_002718"
     CDS             4473..5111
                     /locus_tag="ACIUS0_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGB0220412.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5108..5845
                     /locus_tag="ACIUS0_002719"
     CDS             5108..5845
                     /locus_tag="ACIUS0_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGB0220413.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5845..7941
                     /locus_tag="ACIUS0_002720"
     CDS             5845..7941
                     /locus_tag="ACIUS0_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGB0220414.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7988..8266)
                     /locus_tag="ACIUS0_002721"
     CDS             complement(7988..8266)
                     /locus_tag="ACIUS0_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220415.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8267..8410
                     /locus_tag="ACIUS0_002722"
     CDS             8267..8410
                     /locus_tag="ACIUS0_002722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220416.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8536..8946
                     /gene="psiE"
                     /locus_tag="ACIUS0_002723"
     CDS             8536..8946
                     /gene="psiE"
                     /locus_tag="ACIUS0_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGB0220417.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACIUS0_002724"
     CDS             complement(8990..10465)
                     /gene="xylE"
                     /locus_tag="ACIUS0_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGB0220418.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACIUS0_002725"
     CDS             complement(10836..11726)
                     /gene="malG"
                     /locus_tag="ACIUS0_002725"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGB0220419.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACIUS0_002726"
     CDS             complement(11741..13285)
                     /gene="malF"
                     /locus_tag="ACIUS0_002726"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGB0220420.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACIUS0_002727"
     CDS             complement(13439..14629)
                     /gene="malE"
                     /locus_tag="ACIUS0_002727"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGB0220421.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14994..16109
                     /gene="malK"
                     /locus_tag="ACIUS0_002728"
     CDS             14994..16109
                     /gene="malK"
                     /locus_tag="ACIUS0_002728"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGB0220422.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16181..17521
                     /gene="lamB"
                     /locus_tag="ACIUS0_002729"
     CDS             16181..17521
                     /gene="lamB"
                     /locus_tag="ACIUS0_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGB0220423.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17673..18593
                     /gene="malM"
                     /locus_tag="ACIUS0_002730"
     CDS             17673..18593
                     /gene="malM"
                     /locus_tag="ACIUS0_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGB0220424.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            18822..>18914
                     /locus_tag="ACIUS0_002731"
     CDS             18822..>18914
                     /locus_tag="ACIUS0_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220425.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLL"
BASE COUNT         4717 a         4879 c         4899 g         4419 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgcagatta attacttttg ttttttattt taagcttatg atttttattg ttatttaaat
     3961 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
     4021 cccgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
     4081 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
     4141 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
     4201 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
     4261 accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg gtggcggtga tggttctaat
     4321 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
     4381 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
     4441 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
     4501 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
     4561 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
     4621 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
     4681 aagacggaca acaaaaatgg gtcactcagg atcaagccac tcttgtcaca caacatggtc
     4741 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     4801 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     4861 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     4921 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     4981 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     5041 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     5101 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     5161 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     5221 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     5281 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     5341 gctgacacgg ctggcagaac agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     5401 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     5461 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     5521 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     5581 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     5641 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     5701 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     5761 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     5821 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     5881 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     5941 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     6001 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     6061 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     6121 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     6181 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     6241 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     6301 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     6361 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     6421 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     6481 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     6541 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     6601 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     6661 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     6721 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     6781 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     6841 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     6901 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     6961 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     7021 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     7081 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     7141 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     7201 gatttgtggc tgacggacca tctactgacc accggcagcc tgtttgcaaa tcttgccaac
     7261 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     7321 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     7381 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     7441 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     7501 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     7561 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     7621 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     7681 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     7741 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     7801 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     7861 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     7921 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     7981 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     8041 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     8101 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     8161 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagcag
     8221 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     8281 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     8341 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     8401 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     8461 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     8521 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     8581 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     8641 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     8701 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     8761 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     8821 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     8881 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     8941 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     9001 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     9061 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     9121 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     9181 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     9241 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     9301 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     9361 ccgatgcctg agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagtg
     9421 cgccgataat ttgcagtggc ttacgaccaa atttatccac agtcataatt gccagtacgg
     9481 taaaggtgag gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg
     9541 cccccagcgt tttgaacact tccggcgcgt agtacagcac cacattgatg ccgacaaatt
     9601 gctggaagat ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac
     9661 gaccaccggt tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg
     9721 caagcgtgtt gcccataatt ttgcgcagga tactttccgt ctgttcttgc ttgccgcgtg
     9781 acatcagcca gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag
     9841 ggatacattc cgaggcaaac atataacgcc tgccgtcagt attcagccag ctggcatcac
     9901 cggaacgggc aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa
     9961 actggttaaa tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca
    10021 ttggtgaaag cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa
    10081 attccgggac ataacctgcc agataaatag gtactgtgtt gtccgggttt atagaggtaa
    10141 aaccaagttc tggccaggca gaacctacac cagaaataaa aaacaggaca gcagaaatct
    10201 taagtgaatc acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga
    10261 tgcaaccaat cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac
    10321 ttaagttttg tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg
    10381 cggtgtcgta gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat
    10441 aactggaatt atactgggta ttcattcaga cctgccttag accattctga tcttttatca
    10501 gtgcattgat gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt
    10561 acgtaatgtc ttttctgtga tcttaattgt gataattatc aaaaattgat gaaacaatac
    10621 tattgccgtg actcagagca cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa
    10681 aaataataag ttctatgatg ggaatattaa ccggcgaacg attcagattg cagacgaaag
    10741 aaaaaaaggc gctccgtgga gcgccgaata atagtcacaa gttgggataa cgtaagttga
    10801 gggtgcagcg gcataacatt ggcagaacaa catctttaac ctttcacacc acctgccgtc
    10861 aggccgttga ccagccagcg ttgagccagc aagaagacga tggtgatcgg taatgcagac
    10921 atcacggcag cggcggcaaa gtcaccccac aggtagtttt gcgggttgag gtattgctgc
    10981 atccccacgg ccagggtgta gctgtttacg tcacgcagta acagcgacgc gaccggaact
    11041 tcagtaatgg cagcgataaa cgacaggatg aataccaccg ccagaatcgg tactgacagc
    11101 ggcaacagga caaggcggaa ggcctgccac ggtgtcgcac catccagcgc agccgcttct
    11161 tccagcgaac tgtcgatggt ttcgaaatag cctttgatgg tccagacatg cagcgcaatc
    11221 ccacccagat acgcgaaaat tacgccgccg tgagtattca ggccaatgaa tggaatgtac
    11281 tcacccagac gatcaaacaa cgcatacaac gcgaccagtg aaagtactgc cgggaacatc
    11341 tggaaaatca gcattccttt cagcagcgtc gctttgcctg gaaagcgcat acgggcgaaa
    11401 gcgtaggcgc aggtggtgga gagcgccaca atgccaatcg cggaaatccc agcgaccttt
    11461 accgagttcc acagccacag cagtaccggg aatggcggtg gcgtaatgcg accatcagcc
    11521 tgttcaacgc taaaacctaa cgccagtttc cagtgatccc aggagatttg ctccgggatc
    11581 aggctaccgg tcgcgaagtt tccctgacgc agcgagatag cgacgaccat cagcagcggg
    11641 aacataatcg ccgcgataaa aagtagcagt agcaggtgag taataaacaa acgtgctttt
    11701 tgcgatttcg gttggaccat tgccattttt gttatctccc ttaatcaaac ttcattcgcg
    11761 tggctttcag gttcactatc gccagcgcac ccaccagcag gaagatcagc gtggcaattg
    11821 ctgccgccag accgaagtcc tgacccccgc cgccttcaaa agcgatacgg taggtgtagt
    11881 taacaagcag gtcggtataa ccggctggcg tggtcgtgcc aagacgatcc gggccgccgt
    11941 tggttaacag ctgaatcagc acgaagttgt taaagttaaa ggcgaagctg gcgatcatca
    12001 gcggcgtcag cggtttaatc agcagcggca gcgtaatctt aaagaagttc tggaacggac
    12061 ctgcgccatc cattgctgag gcttcataca aatcgtccgg aatcgctttc agcaagccca
    12121 tgcagaggat catcatgtac ggataaccca gccaggtatt gacgataatc agcatcgtgc
    12181 gagcggtggt cggatcgctg aaccaggcgg gcttcacgcc aaacagcgcg ctcaacatca
    12241 tgttgatttc accgaagctc tggttaaaca atcctttgaa aatcaagatt gaaatgaacg
    12301 atggcaccgc gtagggcaga atcagcagga cgcgatagac cgctttgccg cgcaacgctt
    12361 cccactgcac cagacatgcc agaaccatgc cgaccgccac cgttaaaaag acagtgatca
    12421 gcgagaacac cacggtccag acgaaaatgg cgaggaacgg tttctgaatg ccttcgtcgg
    12481 taaagacgcg ggtaaagttt ttccagccgg tggtcacggt gtaaccgggg cttagctttt
    12541 catcccccca gttgccgtcg gcggtaatgg actggtaaaa gccaatttgg ttattcggac
    12601 gatatttcac gccactctga ttattcgtca acgtgccgtc accgtcgagt gtgtagagcg
    12661 gctgcgtgcc agaaaactgg cgcagggagc tcatcatcac tttgttgcca tccggcagaa
    12721 tggcggtaat gtcactcagc gcctgacgat tctgggtaat cacgcgcaga ttcgcgcgtt
    12781 cgccttcggg ctgggcggtc gtttctttca gttgcagttt ttgctcgccg ccaaatttaa
    12841 acgcgtcgga gaggtaattt ttgccggttt cgccgtcgct gagcgccagt tgccactcat
    12901 cgcccgccgg gtaaagacca aagttatagg ttttgcctgc ttgccaggag cgatctaaca
    12961 acacttcctg cgcacgttca aaagtcagct ggttagtgct gctgtagttg gtgaaggcaa
    13021 tggcgatggt gcagaccaga gggaagagga cgaataatcc cattccagcc attcccgggt
    13081 aaacatagcg ccaggcgtag gctttacgat tggcgaaaat atacagcccc gctgaactca
    13141 atatcagcgt ggtaatggcg aacaggtatt ccccttgtgc gtacattaaa acaacaaggt
    13201 aacccaccag caggccgagc agacctagca ctgaccattt cagcgcgtcg ctttgccacc
    13261 aatgtttctt tttaatgaca tccatggggt tcttcctcat tccaggacgg ataaggcttt
    13321 cacgccttat ccgacaacaa ctgcctgatg cgacactgat gcgtcttatc aggcctacat
    13381 gcgtttcagt tttgtaggcc ggacaaggcg ttcacgccgc atccggcatt tcacagcatt
    13441 acttggtgat acgagtctgc gcgtctttca gggcttcatc gacagtctga cgaccgctgg
    13501 cggcgttgat caccgcagta cgtacggcat accagaaagc ggacatctgc gggatgttcg
    13561 gcatgatttc acctttctgg gcgttttcca tggtggcggc aatacgtgga tctttcgcca
    13621 actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta ttaaccgctt
    13681 ccagaccttc atcagtcagc agatagtttt cgaggaactc tttcgccagc tctttgttcg
    13741 gactggcggc gttaataccg gtgctcagca cgccaacgaa cggtttagat ggttgaccct
    13801 tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg ttggaccatg
    13861 cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct tctgcgatgg
    13921 agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg aaggtcagac
    13981 ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg ccgttttcat
    14041 acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac ggttcttgca
    14101 ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc gccgggatct
    14161 cttcccaggt ttttggcggg ttcggcagca ggtctttgtt ataaatcagc gataacgctt
    14221 caacagcgat tgggtaagca atcagcttgc cgttgtaacg tacggcatcc catgtaaacg
    14281 gatacagctt gtcctggaac gctttgtccg gggtgatttc agccaacagg ccagattgag
    14341 cgtagccacc aaagcggtcg tgcgcccaga agataatgtc agggccatcg ccagttgccg
    14401 caacctgagg gaatttctct tccagtttat ccggatgctc aacggtgact ttaattccgg
    14461 tatctttctc gaatttctta ccgacttcag cgagaccgtt atagccttta tcgccgttaa
    14521 tccagattac cagtttacct tcttcgattt tggcgagagc cgaggcggaa aacatcatcg
    14581 tcgttaatgc ggataatgcg aggatgcgtg cacctgtttt tattttcata atctatggtc
    14641 cttgttggtg aagtgctcgt gaaaacacct aaacggactc tagtttcttt atacggcaac
    14701 ctctttccat cctccttgcc cctacgcccc accgttgctt tgtgtgatct ctgttacaga
    14761 attggcggta atgtggggat gcgcacataa aatcgccacg atttttgcaa gcaacatcac
    14821 gaaattcctt acatgacctc ggtttagttc acagaagccg cgttctcatc ctcccgcctc
    14881 ctcccccata aaaaagccag ggggtggagg atttaagcca tctcctgatg acgcatagtc
    14941 agcccatcat gaatgttgct gtcgatgaca ggttgttaca aagggagaag ggcatggcga
    15001 gcgtacagct gcaaaatgta acgaaagcct ggggcgaggt cgtggtatcg aaagatatca
    15061 atctcgatat ccatgaaggt gaattcgtgg tgtttgtcgg accgtctggc tgcggtaaat
    15121 cgactttact gcgcatgatt gccgggcttg agacgatcac cagcggcgac ctgttcatcg
    15181 gtgagaaacg gatgaatgac actccgccag cagaacgcgg cgttggtatg gtgtttcagt
    15241 cttacgcgct ctatccccac ctgtcagtag cagaaaacat gtcatttggc ctgaaactgg
    15301 ctggcgcaaa aaaagaggtg attaaccaac gcgtcaacca ggtggcggaa gtgctgcaac
    15361 tggcgcattt gctggatcgc aaaccgaaag cgctctccgg tggtcagcgt cagcgcgtgg
    15421 cgattggccg tacgctggtg gccgagccaa gcgtattttt gctcgatgaa ccgctctcca
    15481 acctcgatgc tgcactgcgt gtgcaaatgc gtatcgaaat ctcccgtctg cataaacgcc
    15541 tgggccgcac aatgatttac gtcacccacg atcaggtcga agcgatgacg ctggccgaca
    15601 aaatcgtggt gctggacgcc ggtcgcgtgg cacaggttgg gaaaccgctg gaactgtacc
    15661 actatccggc agaccgtttt gtcgccggat ttatcggttc gccaaagatg aacttcctgc
    15721 cggtaaaagt gaccgccacc gcaatcgatc aagtgcaggt ggagctgccg atgccaaatc
    15781 gtcaacaagt ctggctgcct gttgaaagcc gtgatgtcca ggttggagcc aatatgtcgc
    15841 tgggtattcg cccggaacat ctactgccga gtgatatcgc tgacgtcatc cttgagggtg
    15901 aagttcaggt cgtcgagcaa ctcggcaacg aaacccaaat ctatatccag atcccttcca
    15961 ttcgtcaaaa cctggtgtac cgccagaacg acgtggtgtt ggtagaagaa ggtgccacat
    16021 tcgctatcgg cctgccgcca gagcgttgcc atctgttccg tgaggatggc actgcatgtc
    16081 gtcgactgca taaggagccg ggcgtttaag caccccacaa aacacacaaa gcctgtcaca
    16141 ggtgatgtga aaaaagaaaa gcaatgactc aggagataga atgatgatta ctctgcgcaa
    16201 acttcctctg gcggttgccg tcgcagcggg cgtaatgtct gctcaggcaa tggctgttga
    16261 tttccacggc tatgcacgtt ccggtattgg ctggacaggt agcggcggtg aacaacagtg
    16321 tttccagact accggtgctc aaagtaaata ccgtcttggc aacgaatgtg aaacttatgc
    16381 tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat aagagcttct atttcgacac
    16441 taacgtggcc tattccgtcg cgcaacagaa tgactgggaa gctaccgatc cggccttccg
    16501 tgaagcaaac gtgcagggaa aaaacctgat cgaatggctg ccaggttcca ccatctgggc
    16561 aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc gacttctact actgggatat
    16621 ttctggtcct ggtgccggtc tggaaaacat cgatgttggc ttcggtaaac tctctctggc
    16681 agcaacccgc tcctctgaag ctggtggttc ttcctctttt gccagcaaca atatttatga
    16741 ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt ttagcgcaga tggaaatcaa
    16801 cccgggcggc acattagaac tgggtgtcga ctacggtcgt gccaacctgc gtgataacta
    16861 tcgtctggtt gatggcgcat cgaaagacgg ctggttattc actgctgaac atactcagag
    16921 tgtcctgaag ggctttaaca agtttgttgt tcagtacgct actgactcga tgacctcgca
    16981 gggtaaaggt ctgtcgcagg gttctggcgt cgcgtttgat aacgaaaaat ttgcctacaa
    17041 tatcaacaac aacggtcaca tgctgcgtat cctcgaccac ggtgcgatct ccatgggcga
    17101 caactgggac atgatgtacg tgggtatgta ccaggatatc aactgggata acgacaacgg
    17161 caccaagtgg tggaccgttg gtattcgccc gatgtacaag tggacgccaa tcatgagcac
    17221 cgtgatggaa atcggctacg acaacgtcga atcccagcgc accggcgaca agaacaatca
    17281 gtacaaaatt accctcgcac aacaatggca ggctggcgac agcatctggt cacgcccggc
    17341 tattcgtgtc ttcgcaacct acgccaagtg ggatgagaaa tggggttacg actacaacgg
    17401 cgatagcaag gttaacccga actacggcaa agccgttcct gctgatttca acggcggcag
    17461 cttcggtcgt ggcgacagcg acgagtggac cttcggtgcc cagatggaaa tctggtggta
    17521 atagcaaaac ctgggccgga taaggcgttt acgccgcatc cggcataaaa acaggttgtc
    17581 attatctgaa aggggcgaaa gcccctctga ttatcgggtt tagcgcgcta ttgcctggct
    17641 accgctgagc tccagatttt gaggtgaaaa caatgaaaat gaataaaagt ctcatcgccc
    17701 tctgtttatc agcagggtta ctggcaagcg cgcctggaat tagccttgcc gatgttaact
    17761 acgtaccgca aaacaccagc gacgcgccag ccattccatc tgccgcgctg caacaactca
    17821 cctggacacc ggtcgatcaa tctaaaaccc agaccaccca actggcgacc ggcggccaac
    17881 aactgaacgt tcccggcatc agtggtccgg ttgctgcgta cagcgtcccg gcaaacattg
    17941 gcgaactgac cctgacgctg accagcgaag tgaacaaaca aaccagcgtt tttgcgccga
    18001 acgtgctgat tcttgatcag aacatgaccc catcagcctt cttccccagc agttatttca
    18061 cctaccagga actaggcgtg atgagtgcag atcggctgga aggcgttatg cgcctgacac
    18121 cggcgttggg gcagcaaaaa ctttatgttc tggtctttac cacggaaaaa gatctccagc
    18181 agacgaccca actgctcgac ccggctaaag cctatgccaa aggcgtcggt aactcgatcc
    18241 cggatattcc cgatccggtt gctcgtcata ccagcgatgg cttactgaaa ctgaaagtca
    18301 aaacgaactc cagctccagc gtgttggtag gaccgctatt tggttcttcc gctccagctc
    18361 cggttacggt aggcaacacg gcggcaccag ctgtggctgc acctgctccg gtgccggtga
    18421 agaaaagcga gccgatgctc aacgacacgg aaagttattt taataccgcg atcaaaaacg
    18481 ctgtcgcgaa aggtgatgtt gataaggcgt taaaactgct tgatgaagct gaacgcctgg
    18541 gatcgacatc tgcccgttcc acctttatca gcagtgtaaa aggcaagggg taattacgcc
    18601 ccacagtgct gattttgcaa caactggtgc gtctcctggc gcaccttttt ttatgcttcc
    18661 ttcctgggat atgagcgatt ttttatagta cttcacttct tcttcactaa gaatgtccat
    18721 tatttcaatg cattatcaga gattcttttc ctttcgccgg tagtgtctgg acgctcaggc
    18781 tacttttcca ggttatttta tttctgtatt acagaggttt tatgataggt catatcacaa
    18841 attctggcgg caataactct ttgatgaaac atgatgtggt gcaaggaaat aatatagtag
    18901 atcttgattt acta
//
LOCUS       DBBBSB010000080        18803 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.080, whole genome shotgun sequence.
ACCESSION   DBBBSB010000080 DBBBSB010000000
VERSION     DBBBSB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.080"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(139..3654)
                     /locus_tag="ACIUS0_002732"
     CDS             complement(139..3654)
                     /locus_tag="ACIUS0_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HGB0220426.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="ACIUS0_002733"
     CDS             complement(4107..5369)
                     /locus_tag="ACIUS0_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0220427.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="ACIUS0_002734"
     tRNA            complement(5567..5642)
                     /locus_tag="ACIUS0_002734"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="ACIUS0_002735"
     CDS             complement(5748..6455)
                     /locus_tag="ACIUS0_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGB0220428.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6608..6691
                     /gene="yqgH"
                     /locus_tag="ACIUS0_002736"
     CDS             6608..6691
                     /gene="yqgH"
                     /locus_tag="ACIUS0_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGB0220429.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="ACIUS0_002737"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="ACIUS0_002737"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGB0220430.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACIUS0_002738"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="ACIUS0_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGB0220431.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACIUS0_002739"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="ACIUS0_002739"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGB0220432.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="ACIUS0_002740"
     CDS             complement(11639..11914)
                     /locus_tag="ACIUS0_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGB0220433.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACIUS0_002741"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="ACIUS0_002741"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGB0220434.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="ACIUS0_002742"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="ACIUS0_002742"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGB0220435.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="ACIUS0_002743"
     CDS             13874..14200
                     /locus_tag="ACIUS0_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGB0220436.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="ACIUS0_002744"
     CDS             14384..15103
                     /locus_tag="ACIUS0_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGB0220437.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="ACIUS0_002745"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="ACIUS0_002745"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGB0220438.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="ACIUS0_002746"
     CDS             16442..17404
                     /locus_tag="ACIUS0_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGB0220439.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="ACIUS0_002747"
     CDS             complement(17505..>18803)
                     /locus_tag="ACIUS0_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGB0220440.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DBBBSB010000081        18759 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.081, whole genome shotgun sequence.
ACCESSION   DBBBSB010000081 DBBBSB010000000
VERSION     DBBBSB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.081"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            375..578
                     /locus_tag="ACIUS0_002748"
                     /pseudo
     CDS             375..578
                     /locus_tag="ACIUS0_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            597..3983
                     /locus_tag="ACIUS0_002749"
     CDS             597..3983
                     /locus_tag="ACIUS0_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGB0220441.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3986..4366
                     /locus_tag="ACIUS0_002750"
     CDS             3986..4366
                     /locus_tag="ACIUS0_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220442.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(4421..4708)
                     /locus_tag="ACIUS0_002751"
     CDS             complement(4421..4708)
                     /locus_tag="ACIUS0_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HGB0220443.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            4837..5346
                     /locus_tag="ACIUS0_002752"
     CDS             4837..5346
                     /locus_tag="ACIUS0_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGB0220444.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            5343..6761
                     /gene="phrB"
                     /locus_tag="ACIUS0_002753"
     CDS             5343..6761
                     /gene="phrB"
                     /locus_tag="ACIUS0_002753"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGB0220445.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(6911..8392)
                     /gene="dtpD"
                     /locus_tag="ACIUS0_002754"
     CDS             complement(6911..8392)
                     /gene="dtpD"
                     /locus_tag="ACIUS0_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGB0220446.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            8663..9406
                     /gene="ybgI"
                     /locus_tag="ACIUS0_002755"
     CDS             8663..9406
                     /gene="ybgI"
                     /locus_tag="ACIUS0_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGB0220447.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9429..10085
                     /gene="pxpB"
                     /locus_tag="ACIUS0_002756"
     CDS             9429..10085
                     /gene="pxpB"
                     /locus_tag="ACIUS0_002756"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGB0220448.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            10079..11011
                     /gene="pxpC"
                     /locus_tag="ACIUS0_002757"
                     /pseudo
     CDS             10079..11011
                     /gene="pxpC"
                     /locus_tag="ACIUS0_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            11001..11735
                     /gene="pxpA"
                     /locus_tag="ACIUS0_002758"
     CDS             11001..11735
                     /gene="pxpA"
                     /locus_tag="ACIUS0_002758"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGB0220449.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            11771..12562
                     /gene="nei"
                     /locus_tag="ACIUS0_002759"
     CDS             11771..12562
                     /gene="nei"
                     /locus_tag="ACIUS0_002759"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGB0220450.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(12559..13605)
                     /locus_tag="ACIUS0_002760"
     CDS             complement(12559..13605)
                     /locus_tag="ACIUS0_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGB0220451.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(13757..14833)
                     /locus_tag="ACIUS0_002761"
                     /pseudo
     CDS             complement(13757..14833)
                     /locus_tag="ACIUS0_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(14830..15561)
                     /locus_tag="ACIUS0_002762"
     CDS             complement(14830..15561)
                     /locus_tag="ACIUS0_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGB0220452.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(15576..18035)
                     /locus_tag="ACIUS0_002763"
     CDS             complement(15576..18035)
                     /locus_tag="ACIUS0_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGB0220453.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(18086..18652)
                     /locus_tag="ACIUS0_002764"
     CDS             complement(18086..18652)
                     /locus_tag="ACIUS0_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0220454.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4649 a         4478 c         5164 g         4468 t
ORIGIN      
        1 gttgttaata aaaagcttat agatttatag ttgataatta aaacgcatcc gatatttcag
       61 ctttttctat gcaagataca attttataag gtgtccattt ttatatggag gcggtagcgt
      121 gtctgttact agtggtttta tttgcatagt gaatatattg ggattgttaa atacataact
      181 atgtatccgc tctggtcgtt gtaaatattg ctaattgctt aatattatta gtgattatga
      241 ctggaaagat aaagtgtgct gtaaagcggt gatgggtatt acattcatga ttaaaaaaac
      301 agaacctgtc aataaataat tcctttatta aaacggacta tttacattat gacggaatat
      361 tatctcaacg aggcggtttg gaaatacaga gccagtttct ccggggtaac gagcaggtgt
      421 accagtgtca ggtggcattt gtattgccgg gtgagcgggt catgatggcc ttcacctacg
      481 ccagaaccac accgctgacc ccggcagata tgacgcgctg ggctgaaatt aaaaagaatc
      541 tgcgcttcag gatgcggcag gaagtcagga cgaattaatc atacagggaa cgggacatgg
      601 aagaggcatt ctgggcggca agacagggtg atgccctgct gcatacgtcg tttatggcag
      661 atgtgctggg ggcggtgctg gaggtggccg caaatgtcgt tattgatgcc ctcattgtcg
      721 gcacaacctc gttactggcg acgtcagtgg ggatcacgat ggggtgcacg gccgtcgtgc
      781 tgaccggttt tgtggcgggt gtggcgatgg tgtacaccgg cgtgacagaa aaggtcagcg
      841 aagcctgttc agccctcgca aatatgcttt tcccgcctca gattgaaggt tacatcctca
      901 ccggctctgg tgacacatgg atcaacagca aacctgccgc gcgggcagcg gcgactgcgg
      961 catcgcggca ggacatagag gcgcaggaag cgcaggcgaa ggcagaacag gaagaagcgc
     1021 agcgtcagga ggatgccaga acgttccggg atgtggccgg ggaatatctg gaaatggcgg
     1081 gggccatagc cctggcggtt aacccggtga cagggcctgt gctgatgtcc cgggctctgc
     1141 acgaccagct cagtacggaa gaagggcgtt ctgaaatggt ggatggcgtg acacattttg
     1201 tgtcggaact atggcagccc actgtggcca gtgcggcccc gggatccacc ccgacccctg
     1261 acgataaaat cgactgccat aaacatcctt cctcactgac gcagtttctc gcacaaaaaa
     1321 aagccgcctt tctggatgac cccctgggaa cggcccttaa cctgctgaat ccgggcggta
     1381 tgctggaaac cgcctttcag ggagtgaatg cggttattgg cagcatcagt aatctgttca
     1441 aaggcgatga cgagccgccc gcagcggaat atatcgcgga aggcgcgcgg gatgtgcgta
     1501 tcaacagcca gccggcgaca cgcagcgggg cacgctgtac ctgcgaggcc agggtggtga
     1561 atgagccggg gaacggggcg tttgtgtcac cggatgtgcg cattggcggt ccgttactgg
     1621 tggtcaggga tatcagaagc ggcaggtcgc agataaccct ggtggcaacc gtggcgctga
     1681 tgtttcttcg cccgggcaaa atgctttcta aaatcgcctg ttttgctgcc agtgtcggga
     1741 tgagcatgct gacgcagaag gccaccagtg cccttccgca tccagtcaat gccgccaccg
     1801 gggccaaata cctggccgat gacgatgact tcgatttcag tctgccgggc cactttccgc
     1861 tggactggca gcgcgtgtac agcagtcgtg acgaacgtac ggaaggaatg tttggtcagg
     1921 gctggagtgt aatgtatgaa gtcagcctcg tgtgcacacc gggtagcgca gacgaaaact
     1981 gcatgacctt tgtgtcgggc atggggcgac gcttggatat ggaagccgta ctgccggggg
     2041 gcgggtttta cagccccggt gaagggctgg cggtaaggcg tggtgagcag gggcactggc
     2101 ttatcagcag cgatgacggg cagtgttttc tgtttgaggc tgacccgcac cacccgcagc
     2161 gccagcggct gaaaatgctg ggcgaccgta acagcaactg tctgaatctg tactacgacg
     2221 accggggccg cattgtggaa atcagcggcg aacagcagcg tccgtgcatc cgtctgtatt
     2281 acgaactggc ggcgcatccc cggcgggtga cacagattta tcagcatttc cctgagactg
     2341 cacctttact gctgcgtcgt tacagctatg acgaggccgg ccacctgaac ggcgtgtacg
     2401 acagcacggg gcacctgctg cgtgagtttg cttacgacga aaaccactgc atgacgctgc
     2461 accgccagcc gggcggcgaa gggtattatt accagtgggg ctggtatgaa ggtccggatg
     2521 acgcggcatg gcgggtgacg gggcaccaca cggacagcgg tgaacagtac cggctggcgt
     2581 gggacctggc gcagcgcagg ctgtgcgtga cggacggact ggggcgcaca cgttatcacc
     2641 agtgggatgc gcagaatcag gtgacggcgt accaggacga agccggacag gtgacgacat
     2701 tccgctggag cgacgaagaa cgactgttgc tggggatgac cgaccctcag ggcggcaaat
     2761 ggcgctatgt gtatgacctg caggggcata tcacggagac gcacgacccg ctggggcggg
     2821 tggagcaggc gcagtggcac ccggtgtggc accagccgga aacggaggtg gacgcggcgg
     2881 gaaacctgct ggggaggcgc gcgggggaaa gggccacggc agaaaacagc gtggtgccgt
     2941 tcaaccggct gatgtcttac cgtggtgtgc actaccgtta tgacgaattt ggccgtgcgg
     3001 tggaaaagga gggccggagc ggaacgcaga gttaccgtta tgatgcggaa caccggatgg
     3061 tggaagtgac gacagcgcgg gggacatacc ggtatgtgta cgatgcactg gggaggcgga
     3121 cggaaaaaca gcacatcagc cctgacggta agccgtacaa ccggacaaaa tttttatggg
     3181 acggaatgcg gctggcgcag gagagcaggc cggagggaac aggcagcctg tacatctaca
     3241 gggaccaggg gagttatgaa ccgctggcgc gggtggataa ggcgggcaaa gagggcccga
     3301 accggatact gtattttcac acggatgtga acggcgcgcc ggaagagatg acggacagcg
     3361 acgggaaaat cgtgtgggag acggggtatc aggtctgggg taacacgata caggagaagg
     3421 accacggcgg ggtggagcag aacctgcgct accagggaca gtacctcgac agggaaacgg
     3481 ggctgcacta caatctgcac aggtactatg atccggatgt cgggcggttc atggtaactg
     3541 acccgatagg gttgagaggc ggtttaaatc tctacagtta tgcacctaat cctctaaaat
     3601 atgctgatcc acttggatta actccatgtg ctgttagtaa ccaaaaagcg aatcgtttat
     3661 tagatagtag tgaaaccaaa gttactgtta ggtctagaag tgacgctgaa caattgttta
     3721 tggatagata tttaggtcat aattataaaa atatgacagg tgagtcaggc cccagcacta
     3781 agaacttaat ggaatatctt actgaaaata aaacaaaagc aggttcatat cattgggatg
     3841 atataaaaga tccttctgta acaaaacctt catatagagt gtcaggtcat gggccaggaa
     3901 atcctgatgg tgatttacca catttacaag ttcatcaaca tggtggaagt gttagacata
     3961 tatttttccc gtgggagaca taataatgaa caatctcaat acattattaa gtggaaaggt
     4021 tttgctcaaa gaaattagtc cattaatgcc tggatataga acatggattg aaatttcatt
     4081 gatcgatgaa ttaaaaccat cttatccttt tagattagat gaatatgcta tgatagggca
     4141 ttcaccttat gccaatgaat gtaataaaga tgaagctaaa tttaaactac gaatatcatc
     4201 atttttagct tctgacatag ataatgaata cgatccatca tatgattatg ttggaaaata
     4261 tgaaattatc gcttcactta atgagttaaa aactaggtta agtactcttc atgtaaactt
     4321 ggagcaattt ataaattcat cggaagatga tgagtatcca ttataataag acagaataaa
     4381 aagctaggaa ggttttaaat cttcccagct tttttaatat ttaactttcg ttcagcacac
     4441 taaacatccc atcccgcagt tcctgcacct cgtcactgtc agcgatgatg gtcaggcccc
     4501 cctccgaaat cttcaccgtg acaccccgtc cggtatcaaa tccggcctct ttcagccagt
     4561 cgcccttaag gtgcagactg ggatgttgtg aataataccg ggctataccg gttttgcggt
     4621 cctggtggcg ggctctgaca tagccgactt tatagcggcg ctgtgttttg gaaatagtga
     4681 cttctggcgt aatatcgtgt tcagccatga ttaactacct tgattagttg cttgcgggga
     4741 gcgcgctttc gtaatcttgc cctgctagcg gattaacttc acaaacatca tttaccaacg
     4801 cctacactta ctcctgtata ccgttcagga gcagtaatga atctacaacg atttgatgac
     4861 agcaccctaa tccgtatctt cgcccttcat gagttacatc gactgaaaga acatggctta
     4921 acgcgcgggg cgcttctcga ttatcacagc cgctataaac tcgtctttct ggcccattct
     4981 cagcccgagt accgcaaact tggcccgttt gtggctgata ttcatcagtg gcaaaatctg
     5041 gatgattttt acaaccagta ccgccaacgc gtaattgttt tgctttctca tcccgccaac
     5101 ccgcgcgatc acaccaacgt tttgatgcac gttcagggtt attttcgccc gcatattgat
     5161 tccacagaac gccagcagct ggctgcgctt atcgacagtt atcgccgtgg cgagcaacca
     5221 cttcttgcgc cgctgatgcg tatcaaacac tatatggcgc tttatcctga cgcctggctt
     5281 tcagggcagc gttatttcga actttggccg cgtgtgatta acttgcgcca ttcaggagtt
     5341 ttatgactac ccatctggcc tggtttcgcc aggatttacg tctgcacgat aatctcgcac
     5401 tggctgccgc ctgccgcaat tcgtctgcac gcgtgctggc gctgtatatc gctacaccac
     5461 gcaagtggga gacgcataac atgtcgccgc gtcaggctga acttatcaat gctcaactga
     5521 atgggctaca aatcgcgctg gcggaaaaag gtattccttt attgttccgt gaagtggatg
     5581 actttgtcgc cagtgtcgaa atagttaaac aggtgtgcgc ggaaaacagc gttactcatc
     5641 ttttttataa ctatcagtat gaggtgaatg agcgagcgcg ggatgtggac gttgaaagag
     5701 cgctgcgtaa cgtggtgtgt gaaggatttg atgacagcgt gatcctgccg ccgggggcgg
     5761 tgatgaccgg caatcacgag atgtacaaag tctttacgcc ttttaagaat gcctggctga
     5821 aacggctgcg ggaagggatg ccggagtgcg tcgctgcacc aaaagttcgt agtagcggat
     5881 cgataaagcc cgcgccatcc attacgctga attatcctcg tcagtctttc gatactgcgc
     5941 atttcccggt ggaagaaaaa gcggcgattg cgcaattacg ccagttttgc gagaacggtg
     6001 ctggagagta tgagcaacaa cgagattttc cggcagtgga aggcaccagc cgtttgtccg
     6061 ccagcttggc aacgggcggg ttatcgcctc gccagtgttt gcatcgcttg ttggcggaac
     6121 agccgcaggc gctggacggt ggggccggta gtgtctggct tagtgagctg atctggcgcg
     6181 agttctaccg tcatctgatg acgtattacc cctcgttgtg taaacatcgt ccatttattg
     6241 cctggacgga tcgtgtgcag tggcaaagca atcccgcaca tttacaggcc tggcagaaag
     6301 gcaaaacggg atacccgatt attgatgccg ctatgcgtca gcttaacagc actggctgga
     6361 tgcataaccg gctacggatg attacagcca gttttctggt gaaagatttg ttgatcgact
     6421 ggcgcgaagg cgagcgatat ttcatgtcgc agctgattga tggtgatttg gcagctaata
     6481 acggtggctg gcagtgggcg gcttctacgg gtactgatgc tgcgccgtat tttcgtattt
     6541 tcaatccgat aactcagggc gagaaatttg accgtgaggg cgagtttatt cgtcgatggt
     6601 taccggagct gcgcgatgta ccagggaaag cggtgcatga gccgtggaag tgggcgcaga
     6661 aagcaggtgt gaagctggat tatccgcaac cgatagtcga gcacaaagag gcgagagtac
     6721 aaacgttggc agcgtatgag gcggcgcgga aggggaaata acagacgcgt caggcaatcg
     6781 agcccagatg ccggatgcgg cgtgaacgcc taatccagcc tacaaatttg tgcaaattca
     6841 ataaattgca ggaaatacgt aggcctgata agacgcgtca agcgtcgcat caggcatcgg
     6901 tgctggccta ttatgactcc agcgccagcg cgcggtttct gaatttcagc gcctgataca
     6961 gccaaatcat cagtaccagt cctacacacg ccagtgcgcc ccaggtaatt tgatcaaata
     7021 cttcaatata tgcattgatg gagtagttga tcgccccgga agcatcaaac gaagcctgcg
     7081 atgtctgatc ggcaatcacg cccgccagat agttcgcgat cgcgccagaa agcagcatgt
     7141 agatgccggt taatacgccg gtcacgcccg gaatttcaat gcgcgtaatt tgcgacatgg
     7201 caaccgggtc gataaacagt tccgcaaagc ccatcaccgc gaggcctaac accatcagtg
     7261 gcagagaaga gtgaccatac attgcggacc agcgggcgct taaggtcaga atgcagaatc
     7321 cggcgctcat caggccaagg ccaagagcaa attttcccca gatgcgcacg gtacgattac
     7381 ctgcgacgct ttctttaacc acccacgcca ggaacacacc gcacagcata actgcgaagg
     7441 cattaatcga ctggaacatc gcggtcggaa cggtataacc aaacatatcg cggttaacga
     7501 agcggtcgat ataaaggcta atcgagctac cgccctgttg tgcgaaggcc cagaacaaca
     7561 tactgaagaa ggtgagcgtc acaatcagcc ccagctcctt ccgctgtttc tggttttctg
     7621 ctttgcgata aatttttgcc agtacaccca ggccaataat agtcgcgaca attaaggcgt
     7681 ataccgacca ctctttccag aacagcacgg taatcagtgc aggcgttgcc accagcagaa
     7741 ccagcagcca tccccagttc ggcaggagaa agtttgtcgc acgcagtact tttttgttaa
     7801 cgccgcgggt atgagtgaaa tgacgattgc cacataagaa aatgaccaga cccgcgatca
     7861 ttccaaccgc cgccaggcca aagcccatcg cccagctgta ctcttcctgg gcgtaaccac
     7921 aggcgatagg tgcgataata gaccccacgt tacccgccgc atacatcagc gagaaaccgc
     7981 catcacgacg cggatcggtt ggctcataca gctcgccgag cagacagctt acgttagatt
     8041 taaacaggcc atagccgcag acgataatcg ccagggacag atagaggaat gacggatgga
     8101 tctcactggc acccagcacc acatgaccga tcgccatcaa caacgccccc agcatcaccg
     8161 ccatgcgatt gccgagaact ttatccgcca aaaagccacc gaggattggc gtgacataca
     8221 ccagcgaaca gtaggcgcta aataactcgt aggcgtgagt atcgttgtat tttagttgat
     8281 tggtgagata gagaatcagc agggcacgca tgccgtaaaa gctaaaatat tcccagattt
     8341 gcagcgcaac gacatagtaa atagcgcgcg gttgtgatgc gtgtttattc ataataattc
     8401 cacaattgac ggcaacgtca cgtaatagtt gcgcggcgtg cttgtgtttc cttaaagtta
     8461 aaactttgat acagatctga ttatttatgc aatatgctgt ctgattgcat aaatatacat
     8521 tagctgaagc gtgatgatat aaacaatttg cgtcttttgg cgtcgggttt gtttcttaat
     8581 atgtcaaaag attggctatc gacgaacagg cgatgtaacg ctatgttaac ggtgtagcta
     8641 tcagaatacg gaaggcgcga agatgaaaaa caccgaactg gaacaactga ttaacgaaaa
     8701 actgaacagc gcggcgatta gtgattacgc gccgaatggt ttgcaggtgg aaggcaaaga
     8761 gacggtgcaa aaaattgtta ccggtgtcac cgccagccag gctttgctcg atgaggcagt
     8821 gcgtctgggc gcggatgcgg tcattgtgca tcatggctac ttctggaaag gagagtctcc
     8881 ggtcattcgc ggaatgaagc gtaaccgttt aaaaacgttg ctggcgaatg atatcaacct
     8941 gtatggctgg catttgccgc ttgacgcaca tcctgagctg ggcaataacg cacagctggc
     9001 ggcgttactg gggatcacgg tcatgggcga aattgagccg ttggtgccgt ggggagagct
     9061 gaccatgcct gtgccgggaa tggaactagc ctcctggatt gaagcgcgtc tgggacgtaa
     9121 gccgttatgg tgtggcgata ccggacctga ggtggtccag cgcgtcgcct ggtgcacggg
     9181 cggcgggcaa agttttatcg atagcgccgc gcgttttggc gtggatgctt ttattactgg
     9241 cgaagtttct gaacagacca ttcattcagc ccgcgagcag ggattgcatt tttatgctgc
     9301 aggtcaccat gccactgaac gcggtggtat tcgcgcattg agcgagtggc tgaatgaaaa
     9361 taccgatctt gatgtgacct ttattgatat tcctaatcct gcataacgaa taatcagagg
     9421 gatcgaaagt gcaacgagcg cgttgttatc tgataggtga aacggcggta gtgctggaac
     9481 tggaaccgcc ggtgacgctg gctagccaga aacggatctg gcgactggcg cagcgtctgg
     9541 tggatatgcc gaatgtggtt gaagccattc ccggcatgaa caatatcacg gtgattttgc
     9601 gtaatcctga gtcgctggcg ctggatgcca tagagcgttt gcaacgctgg tgggaggaga
     9661 gcgaggccct ggaacctgag tctcgcttta ttgaaattcc ggtggtttac ggtggtgcag
     9721 gcggaccgga tttggcggtg gtcgcggcgt attgcgggtt gagcgaaaaa caggttgttg
     9781 aattgcactc ctccgtggaa tacgtggtct ggtttttagg ttttcaaccg ggcttcccgt
     9841 atctcgggag tttgccggaa caactacaca cgccacggcg cgctgaaccg cgcttgctcg
     9901 ttccggcagg ttctgtcggg atcggcgggc cgcagactgg tgtttatccg ctggcaacgc
     9961 cgggtggctg gcagttgatt ggtcatacct cactcagcct gtttgatccg gcgcgtgacg
    10021 aacccatctt attacgtccg ggagacagcg tgcgatttgt accgcagaag gagggaatat
    10081 gctgaagatt attcgtgcgg gcatgtacac cactgtgcag gatggcggtc gtcacggttt
    10141 ttgccagtcg ggtatcagcc actgtggcgc actggatatg cctgcgctgc gcattgctaa
    10201 cctgctggtg ggtaatgacg ccaatgcccc cgcgctggag atcacgctcg gtcagttaac
    10261 agttgagttc gaaactgatg ggtggtttgc cctgacgggt gcaggttgcg aagcgcggtt
    10321 ggatgataac gccgtctgga ccggctggcg attgccgatg aaagcaggcc agcgtttaac
    10381 gcttaaacgc ccgcagcacg ggatgcgcag ttatctggcg gtcgcgggtg gtattgatgt
    10441 tccgccggta atggggtcat gcagcaccga tctcaaagtg gggattggcg ggctggaagg
    10501 ccgtttactg aaggatggtg actgactccc gattggcaaa tcgaagcgtg attttatgga
    10561 agcgcagggc gttaaacagc tgctgtgggg caaccgcatt cgcgccttgc cggggccgga
    10621 atatcatgag ttcgatcgcg cctcgcagga tgcattctgg cgttcgccct ggcagcttag
    10681 ctcgcaaagt aaccgaatgg gctatcgttt acaggggcaa attttaaaac gcatcaccga
    10741 tcgcgaactg ttatctcacg gtttgttacc gggcgtggtg caggtgccgc ataacgggca
    10801 gccgattgtg ttgatgaacg acgcacagac caccggtggt tatccacgta ttgcctgtat
    10861 cattgaggct gatatgtacc atctggcgca aattccgctc ggtcagccga ttcattttgt
    10921 ccagtgttca ctggaagagg cactgaaagc gcggcaagat cagcaacgtt attttgaaca
    10981 attagcgtgg cggctgcaca atgaaaattg acctgaacgc cgatctgggc gaaggctgcg
    11041 ccagcgacgc agagctatta acgctggttt cctctgccaa tattgcttgt ggatttcatg
    11101 caggcgatgc gcaaaccatg caggcttgcg tgcgtgaagc aataaaaaat ggtgtcgcga
    11161 ttggcgctca cccgagtttt cccgacaggg aaaattttgg tcgcagcgcc atgcagctgc
    11221 cgccggaaac cgtttacgcc cagacgctgt atcaaattgg cgcgctggca acgattgccc
    11281 gtgcgcaagg cggcgtaatg cgtcatgtca aaccgcacgg catgttgtac aaccaggcgg
    11341 cgaaagaagc acaactggca gacgccatcg ccagagcggt atacgcttgc gatccggcat
    11401 tggttctcgt tgggctggca ggaagcgagc tgattcgtgc aggcaagcaa tatggcctga
    11461 caacgcgcga ggaagtgttt gccgatcgcg gttatcaggc tgacggctcg ctggtgccgc
    11521 gaagtcagcc aggcgcgttg attgaaaacg aagaacaggc gttggcgcaa acgctggaga
    11581 tggtgcaaca cggcagagtc aaaagtatca ccggcgaatg ggcaacggtc gcggcgcaaa
    11641 cggtctgcct gcatggcgac ggcgagcacg ctctggcgtt cgcccgccga ttacgagcta
    11701 catttgccaa aaaggggatt gttgtcgcag cataaccccg cttaataaag aatgaaaaaa
    11761 ggatatcacc atgcctgaag gcccggagat ccgccgtgca gcggataacc tggaggcggc
    11821 gatcaaaggc aaaccactaa ctgatgtctg gtttgccttc ccgcagttaa aaacttatca
    11881 atcacaactt atcggtcaac acgttaccca tgtggaaacg cgtggtaagg cgttgttaac
    11941 tcattttccc aacggtttaa cgctctacag ccataatcag ctttacggcg tctggcgcgt
    12001 ggttgatacc ggcgaagagc cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc
    12061 tgacaaaacc attctgcttt atagcgcctc ggatattgag atgttgaggc cggaacaact
    12121 gaccacgcat ccgtttttac aacgcgttgg tcccgatgtg ctggatccga atctgacgcc
    12181 ggaggtggta aaggaacggt tattgtcgcc gcgctttcgt aaccgtcagt ttgcaggatt
    12241 actgctcgat caggcgtttc tggctgggct tggcaattat ttgcgggtgg agatcctctg
    12301 gcaggttggg ttgactggaa atcataaagc gaaagatctc aatgcggcgc aactggatgc
    12361 actcgcacac gcgttactgg agattcctcg gttttcttac gctacgcggg ggcaggtgga
    12421 tgagaataag catcatgggg cgctgtttcg ctttaaggtt tttcatcgtg atggcgaact
    12481 gtgcgaacgc tgtggcggca tcattgagaa aaccacgttg tcctcgcgcc cgttttactg
    12541 gtgtccaggc tgccagcact aggccgaccg cttcggcgca taggttgaaa taaaccgcgc
    12601 aatggctggc cccgtcagca aaatactgaa caggcgcagg gtttgcatcg ccataataag
    12661 cgccatatcg gcattgctcc ctgcggcgat gaccgccacg gtatcaagcc cgccgggact
    12721 ggtggcgagg taggcagtca taaaatcaat atgcataaac cgggtcagtc cccacgccat
    12781 acctgcacaa atagccagca gagcaaaaat cgacagcagg atttgcggta gcgggcgtag
    12841 tgcccgcagt aagatttgtt tatcgaaacc aagaccaatc cgccagccaa ttgccatata
    12901 cgccatcgcc agcagccatt ccggtagttc gatggtgatg agctgaccag actggagcac
    12961 tgcgcccgcc agcatcggga tgagcatcgt gccggaagga agacgcagca tgcatcccgc
    13021 cgtaccggca acgactgcga gcaaaatcgt aagcaggaga ttaatgctca ctggcgggaa
    13081 ccagacgatt tgctggttaa ccgcttcagc gttatcaccc agcatcatgc gggtgaccag
    13141 aacagcggcc cccgcgacga acagcactcg cagatattgc ataaacgcca ccaggcgaat
    13201 atctgcgccg taatcttgcg ccatagcgac cattgccgct gcgccgcctg gagaagaacc
    13261 ccaggcaccg gtatttccag gcagtgagct atagcgcacc aataaccaac ccacgatggc
    13321 gctggaaagc agcgtcacca gtaaaatagc aagcacgatc ggccagttgg cggccagcgt
    13381 ggtgagaatc gaaccagtga ggttttgcgc aatcatgcag ccaagaatgg cctgcgcggc
    13441 gagaaaagcg gagcggggaa gttgcagagt aattccgcgc atactgaaga tgatcccggc
    13501 gatcatcggc ccaagtaata gcgccgccgg gagatgtacc gcgaggaaac caatagaaag
    13561 gagaagtgat agcacacata acattcccca ctgcaaaact ggcatccttc gctccctcat
    13621 tattttctct ttggtgtatc gatacagcat aaagaaagcc gggctaggaa aaaataacag
    13681 caatcaagga aaaggggaaa atcagcaatt ttctgaaaga gatgcccttt ccggctgcaa
    13741 agggcatcat ggcaaatcag ttatagttaa ttttaaaaag caccactgta tcgaagggcc
    13801 cagcctcgat gggggtacct gcaatggacg aaagttcagc tgtataggtt ttttcgtaga
    13861 ccaacatgtc gctggtgaaa tcggcgtatt cagcatattt attgaaggta taaggcgagg
    13921 cgttttcatc cagcaaacgt agcttcagac cattaccaat caacaatgct ttatcttctt
    13981 caaccaacgt ttcttcggta taaaaggagg aactgacttt aaatccgtcg gtgcattggt
    14041 cattttgtga ttttgtcgtc ttaatactga atgttttcga cattgtgtgg cgacgaatat
    14101 ccagcaaatt aaatttacca aaatcaatca tctgtgtttc cggtgaaatg gaaaaattga
    14161 cgctgcaatc cagcacgcga atgttttcca gaccagtaat atgatatttc aggttttggg
    14221 cagtagggtc ttcgttaacg ctaccggcac cgtcaaattg cacgacgata tagtcgctaa
    14281 gcgtactttg ataatcatgc ggcggcattt ctcgtatttt gacgtataac cgcatgcgag
    14341 cgagaaaggt tcgcgacatg tgaatattag cgggatcacc cgagcaaatt ttgttctgcc
    14401 atcccatcag gggtataaat gtcaatgttc ttactgtcga tacactggtt agtgtcgatg
    14461 cggctttttc cctggttggc gtcataatca acgccattgt aagttactcc gagttgataa
    14521 taacgatcct gcacccccgg gtaggggttt acccatacgt agacatgttc gctttcaaag
    14581 ttgccgttgg tattgttgtc acaatagacg ggaattttaa tatcgtcaga ttcccagatc
    14641 ttattttaat atcgtcagat tcccagatct tatcgccgag tttggcattg cctggtacag
    14701 caaaaggttg aatcgcttct gatttttcta ccgagccacc ggatgaacca aagtagcagt
    14761 tcagggcaag cgcagacttc aacgacagaa ataacagaca gatgacgagc aacactccct
    14821 tgccagcact catttcttcg cctccacggc ttgtaaatgg cattgtccgc tgcagttgag
    14881 cgtcgtcatg cgtaacgcgc cgtaatcatc catataaccg agataaaact ggctgccgct
    14941 ataatttccg gtgtttgtgt tgaccgtact aaaaggcgca accatcacgg ttttgaagcc
    15001 ggggagaacg cctttttcat ttcgtcccag gtaggcaatg gtcagataat acgcggtagg
    15061 atttttcagt gtcagttggt tgccctgctg gctgacctgt aactgcagtt cgaccttttc
    15121 tcctgctttc ttgcgtaatg cagccggtcg ccagaacacc ttgatacgac tctgaatggc
    15181 gacttgcaga acggcatggt cgctgttttt ttccggggcg ggaggaattt cacgcatatt
    15241 gtagaaaaat agcgtttcgc gatcgcccgg taattttgtg gtcgaggctt gctttactat
    15301 ccgtacctgt gttgttgctt tcggttccag acgttgtatc ggcggcaggg cgaccagaag
    15361 atcatcgctt ttctcccctt tttcattttc gagccaggaa taagcgaggt aggggagttt
    15421 atcgctacga ttatccaccc gcagactggt agctttatca ttagcgttaa acacaatacg
    15481 agtgcgatcg ggttgtagtg cagcgtggct gcacagactg gcaaccaaca acaaaagggg
    15541 tagtcctttc ataaatgtca ttttcatttc cttaattaat gacaaggtaa aatcaggcga
    15601 tttgcggtgt cttccatatg ttcaggaaga tggagcgagc aatgctggct atcaccccag
    15661 accacggtaa acttttgatt ttcagcgact ccgcttaacc aggcatgtcc ttcctcgcca
    15721 accatcccca cgctaatgcc gctgtcatcc tggcggatat ccgctccgag gggaggaaac
    15781 tgaccgctgg cgttgcgaat gatgacgtta acgtctttac cggaacggga agccagtgat
    15841 ttgtaaccta tcgcgccttc aatccacgtt tctttgataa cgttttctgc aactgttacg
    15901 ccgtcgggta agtcattcat gttcaccgcc acggtggaag gctggtaact ggaaatcaac
    15961 ggcaccaccg caatgccaaa atggttggtg tagtcgagat tgccctgaac cggaatatct
    16021 gccacgccat cggtgctgac catcaggcgt ggttcattgg tggagctgcg gcgatgaaac
    16081 gctgcaccat attgggttgc ggtgaaagaa ccgctccagc tgctgctgac ggaactgtaa
    16141 tcattggcgg cataggtacc agaaatatcc cactcacccg ctgaactcag gtgctgatag
    16201 ttaccgctca cctgggctcc attgtcagga cggtcggatt gcagtccggc agacatgccc
    16261 cagctattac gttcatccag cgtatcgttc cacgacatgc ggtgtgtggt gctgtggcta
    16321 ctgttttgca tgtcatagcc aacccgacca ccgttaccga aggggagcga aatcgacagg
    16381 taaatctggt tgtcgcgatc tttatcttcg taatgggttg tattgaatga cgtagaaata
    16441 gagatatctc tccagtcacc aatatcaaca ttaaaacctg ccgtgatgtt ggcggtcgtg
    16501 gaggcgtctg cattccacca ggtttgatgt agcaggttgg cgtaaagatt gaggtttagt
    16561 ggggtaatcg gttggcccac agataagctg atcgtctgtt tttcgtcctg cgcatcgctg
    16621 tcgttgtatt tgtgatccag atagttggcg tagctgtgaa aatgacgatc ggagaaacga
    16681 taagcggcga gggaaatcgt gctgttagtg gcatccactt gtttgctgta attaaaacgg
    16741 tagcttaagc cccgctcgtc ctgctgagta tcaaaatggc tactggccca cgtgacatca
    16801 aacgacagcg caccaagcca cagcatattt tgcccaatac ccattgcggc agaatggtag
    16861 tcatcaccag aaagcagcag gccgctgtac agcgaggtgt ttgacagcat cccccaggaa
    16921 acttcattgc taaaaaaggt ttcattttca gtttgatgtg acatggaggg ccgtggctga
    16981 cccgcggcca gtttatagcg aacctgtccc tgacgagtca ggaagggcgt cgatgctgct
    17041 gaaacctgga aattgttcac ccgaccatct tcttccgtca ctttgacatc cagtgtgccc
    17101 tgaacagact gattaaggtc gtcaatgata aatgggcctg gtgggacttt tttctggtaa
    17161 atgacgcggc ctgattgact gatcgtcacc gtggcattgg tctgtgcaat accgctaatt
    17221 tgtggggcgt agccgcgtag ttcccatggc aacattcgat cgtcacttgc cagtgccgcg
    17281 ccggtataag aaaaaccgtc gaaaatattg ggactaaaat ccgtttcgcc gagggttaat
    17341 tttgaaccta attgcggtaa cgggcgaaaa agataggtac gtgatatttc gcctgactgt
    17401 tcatggttat catcgctatc agtatgattc aactgataat cactgcgtaa gcgccatgcc
    17461 cccgtgttga ttccggctgt tccataggcg ttaagattag tgctgctgct gccatcctgt
    17521 gggcggtagc tgctggcaaa caagttgtaa tccatcagga caccggcgac accttctttc
    17581 catgtggagg gtggggtcca gttttctgag tgccacgcca gccaggcttg cggaatacta
    17641 atattcagtt gctgattggc ttgatcgaaa ttgaagagca tttcaggtcg ggagctaaaa
    17701 tcgacgcact gatttatcaa tggtaacgac tgacggactt caggttttaa gccaaattta
    17761 tcgaccagta aatcattgat gcacggaatg gttttatcgt catttttgtg ccagttaatc
    17821 ttttgctcgt tactgatttg gttattattt accgcaacgc taacaaaata ctcaccggga
    17881 gcaatgactc ctttttcctt taataatgaa atatcaatgc ggtcgcgcat tgatttatcg
    17941 agaacattca gattgaattc gaccgccaat gcgcaaggca tcgccacgac caggcaggat
    18001 acaaaagaga gtcgataaat attcacggtg tccatacctg ataaatattt tatgaaaggc
    18061 ggcgatagcg ccgccaaagt aatacttatt tataatccag cacgtaggtt gcattcgccg
    18121 taactgcgcc tggcgttaca ggtgtagcct gatcaatttg ttccatccag gcaaagaaat
    18181 tcagcgtttg ttcgctggag gtcggagcca ggtcgatatc tggagtagcg gtgcctaaaa
    18241 cgatattgct ttgatcttta ttcattaaac gtacgccgac gccggtggct tcacccgtgc
    18301 tggtgttgtt gagaatcgga tctgcgccgg tggttttcgc tgagctatca aaggtgactg
    18361 caactttaga aatcttgcca ccggaaccgt tgctggagtt ttccagatca cagttgacta
    18421 agagcaggtc gacagcaacg gcatcgctgt gatgtgactg attaatgtgg ctggtggtca
    18481 cctgaccgag ttcaacctct ttatcaatat catcctgatg tatttcacac ggtgcttcaa
    18541 taacttcacc tttaaagtga atttcaccgg atccttcatc agccgcataa acaggtgctg
    18601 tgaacagcag agatacggcc agtgcggcca atgttttttg tcctttaaac ataactgagt
    18661 cctttaacag aaaagaaaaa agtatatcgg tgcaagaata tcttatttga ttgtcgaatg
    18721 tttttagttt ttcttaaaaa tattattaat tttattaaa
//
LOCUS       DBBBSB010000082        18111 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.082, whole genome shotgun sequence.
ACCESSION   DBBBSB010000082 DBBBSB010000000
VERSION     DBBBSB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.082"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            63..1154
                     /locus_tag="ACIUS0_002765"
                     /pseudo
     CDS             63..1154
                     /locus_tag="ACIUS0_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1300..2337)
                     /gene="iap"
                     /locus_tag="ACIUS0_002766"
     CDS             complement(1300..2337)
                     /gene="iap"
                     /locus_tag="ACIUS0_002766"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGB0220455.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2589..3497
                     /gene="cysD"
                     /locus_tag="ACIUS0_002767"
     CDS             2589..3497
                     /gene="cysD"
                     /locus_tag="ACIUS0_002767"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGB0220456.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3499..4926
                     /gene="cysN"
                     /locus_tag="ACIUS0_002768"
     CDS             3499..4926
                     /gene="cysN"
                     /locus_tag="ACIUS0_002768"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGB0220457.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4926..5531
                     /gene="cysC"
                     /locus_tag="ACIUS0_002769"
     CDS             4926..5531
                     /gene="cysC"
                     /locus_tag="ACIUS0_002769"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGB0220458.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5581..5904
                     /locus_tag="ACIUS0_002770"
     CDS             5581..5904
                     /locus_tag="ACIUS0_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGB0220459.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6098..6409
                     /gene="ftsB"
                     /locus_tag="ACIUS0_002771"
     CDS             6098..6409
                     /gene="ftsB"
                     /locus_tag="ACIUS0_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGB0220460.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6428..7138
                     /gene="ispD"
                     /locus_tag="ACIUS0_002772"
     CDS             6428..7138
                     /gene="ispD"
                     /locus_tag="ACIUS0_002772"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGB0220461.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7138..7617
                     /gene="ispF"
                     /locus_tag="ACIUS0_002773"
     CDS             7138..7617
                     /gene="ispF"
                     /locus_tag="ACIUS0_002773"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGB0220462.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7614..8663
                     /gene="truD"
                     /locus_tag="ACIUS0_002774"
     CDS             7614..8663
                     /gene="truD"
                     /locus_tag="ACIUS0_002774"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGB0220463.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8644..9405
                     /gene="surE"
                     /locus_tag="ACIUS0_002775"
     CDS             8644..9405
                     /gene="surE"
                     /locus_tag="ACIUS0_002775"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGB0220464.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9399..10025
                     /gene="pcm"
                     /locus_tag="ACIUS0_002776"
     CDS             9399..10025
                     /gene="pcm"
                     /locus_tag="ACIUS0_002776"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGB0220465.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10210..11304
                     /gene="nlpD"
                     /locus_tag="ACIUS0_002777"
     CDS             10210..11304
                     /gene="nlpD"
                     /locus_tag="ACIUS0_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGB0220466.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11367..12359
                     /gene="rpoS"
                     /locus_tag="ACIUS0_002778"
     CDS             11367..12359
                     /gene="rpoS"
                     /locus_tag="ACIUS0_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGB0220467.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12479..12886)
                     /locus_tag="ACIUS0_002779"
     CDS             complement(12479..12886)
                     /locus_tag="ACIUS0_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGB0220468.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            13033..13626
                     /locus_tag="ACIUS0_002780"
     CDS             13033..13626
                     /locus_tag="ACIUS0_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGB0220469.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13626..14573
                     /locus_tag="ACIUS0_002781"
                     /pseudo
     CDS             13626..14573
                     /locus_tag="ACIUS0_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14846..15409)
                     /locus_tag="ACIUS0_002782"
     CDS             complement(14846..15409)
                     /locus_tag="ACIUS0_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220470.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15574..15825
                     /locus_tag="ACIUS0_002783"
     CDS             15574..15825
                     /locus_tag="ACIUS0_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0220471.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15827..17923
                     /locus_tag="ACIUS0_002784"
     CDS             15827..17923
                     /locus_tag="ACIUS0_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGB0220472.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4480 a         4408 c         4846 g         4377 t
ORIGIN      
        1 ttaatttatt cataatgtaa atgttttatt ggctgatata tatcatagat agtttttaaa
       61 atatgcgtaa atatccttta agtttactga aggataaaaa tattgtgact ttctttgatt
      121 tctggggaaa aaaccgacgt ggcgagaaag agggtatcga cgactatcac cttctttgct
      181 ggcatttgct ggatgtggcc gcaatgggct atttaatggt taaaagaaat tgcttcgggc
      241 tggctgatta ttttcgtcaa ttagggattt ctgacaagga acaggcagct cgatttttcg
      301 cctggttgct gtgctggcac gatattggaa aatttgcccg ctcttttcaa caactttacc
      361 tgccccctga attcaagatt caggaaggtg ccagaaaaaa ttacgaaaaa atctctcatt
      421 caacgctggg ctactggttg tggaatcatt atttaagtga atgtcaggag ttacttcctt
      481 catcttcgct ctctccgcgc aaacttaggc gagtgataga aatgtggatg cccgtgacga
      541 cagggcatca tggtcggccc cctgatcgta tggatgaatt ggataatttt ctgcctgaag
      601 acaaagctgc agcacgagat tttctccttg aaatcaagcc actgtttccg cgaatagaga
      661 tccccacatt ctgggatgat gacgagggcg ttgaacttat aaaacaactt tcctggtata
      721 tctctgcaac cgtcgtactt gcagactgga cagggtcgtc aacgcgattt tttccacgcg
      781 tcgcacaggc aatggatatt gaagattact ggcaaaaggc tttagttcag gctcaaaacg
      841 cattaaccgt ctttccgcca caagcagaaa ccgcgccttt caccggaatt aatacgctgt
      901 ttccttttat tgagaatacg acaccgttac agcaaaaggt gctggatctg gatatcaatc
      961 agccaggacc acagttgttt attctggaag atgtgaccgg cgcaggtaaa acagaagcgg
     1021 cgcttatcct ggcgcacagg ttgatggctg cgggtaacgc tcaggggctg ttttttggcc
     1081 tgccaacaat ggcaacggcc aatgccatgt acgatcgact ggtcaaaacc tggcttgctt
     1141 tctattcgcc agaggtgttc cccgctccag cggagataaa ccggcgcaat acggtgcggt
     1201 tttcccggaa gtaaaccgga cgcactggat gcgatgatgg gtatcactta gaattccccg
     1261 cccctgcggt agaactccca gctcccattt tcaaacccat caagacgcct tcgccagctc
     1321 cttcaccaac ggtagcatta tccgcataac gtcacggcag cgacgttcta ttcttccagg
     1381 aagtgcctta tcaatatgtt gttgattatc cagtcttacg tcatgccagc tatttcccgc
     1441 cgggaaggca ggtgtttttg cgcgttgctg ataaccatcc ttatttccaa gattccagtt
     1501 agtcgcttcc accgaaagta cagcaatgcc cgctttgtcg aatatttctg cgtcattaca
     1561 acccccagtg cctttcggat aatttttatt caaacccgga ttggtcgttg cggctattcc
     1621 gtgactgcgt gcaattgcca gcgccctgtc gcgcgttaat ttccttactg cttcaggggt
     1681 ttttacaccg ctgttgaaat acaatttatc gccaacaatt aagttatcga gattaatcac
     1741 cagcagcgta tttttctttt cggtgtcact catccgcttg agtaaattct cagcgcctaa
     1801 ttttccttcc tcttcgccgc tggtcgccac aaatcgaata ccatactcgg taggcgtatt
     1861 tttcaggcgt tctgccagtt ccagcatgac acctaaacct gcggcgttat catctattcc
     1921 ttgtaacgtc agtccgccga gattggcatc tgcgtcagca tcgctcagcg gggcgtaagt
     1981 atccagatgc gccataatga tgatctgctg cggcgctttg ccttcatgag cggcaatcac
     2041 cgtacttcct gtcacgttat gccagttctt gcgattatcg cgggcggtat aaatataccg
     2101 actattaaat gtccgaatat cactgcgata acccatttgc tgaaactgtt ggcgaatata
     2161 atcggcagat aacatttctg ctggagttcc ggtcatgcgt cccggaaaga aagtagcaat
     2221 atgtcgtgcc tgggtattag caaaatcgcc aggtttaggt gacgaggcgt gtacggggag
     2281 aataaagcat acgccgagcg ccagggcagc ggtacggtgg cgcaatgcgg aaaacatagt
     2341 gagtccttaa ataccatgca aattttttta ccgccatagt atgaaactgc cgctgcgcta
     2401 aaacaatttc aaatcttcct aaacgcccga aatccggtgc cttaagcact ttttgatatt
     2461 agctttgcca aatcgttatt ccgttaagga actactcatt ctaattggta atttcattcg
     2521 ttctcttacg ctccctatag tcgaaacatc tgatggcaag aaaatagcgg tattgcaaag
     2581 gaacggttat ggctcaaaaa cgacttactc acctgcggca actggaggcg gaaagcatcc
     2641 acattattcg cgaggtggcg gcagaattct caaatccggt gatgctctac tctatcggta
     2701 aagattccag cgtcatgctg catctggcgc gcaaggcgtt ttatccaggt acgctgcctt
     2761 tcccgttgct gcatgtcgat accggctgga aattccgcga gatgtatgag ttccgcgatc
     2821 gtacggcgaa agcctacggc tgcgagctgc tggtgcataa aaacccggaa ggcgtggcga
     2881 tggggattaa tccattcgtg cacggcagcg cgaaacatac cgatattatg aaaactgaag
     2941 gcctgaaaca ggcgctgaac aaatacggtt ttgatgccgc cttcggtggt gcgcgccgtg
     3001 acgaagagaa atcccgcgct aaagagcgaa tctactcttt ccgtgaccgc ttccatcgct
     3061 gggatccgaa aaatcagcgc ccggagctgt ggcacaacta caacgggcaa attaacaaag
     3121 gcgaaagcat ccgcgtcttc ccgctctcta actggaccga gcaggatatc tggcaataca
     3181 tctggctgga aaatatcgac attgtgccgc tgtatctcgc tgcggaacgt ccggttctgg
     3241 aacgcgacgg tatgttgatg atgattgatg acaaccgtat cgacctgcaa ccgggcgaag
     3301 tgatcaaaaa acggatggtg cgtttccgta cgctgggctg ctggccgctg accggtgcgg
     3361 tggagtcaaa tgcacaaaca ctgccggaaa tcatcgaaga gatgctggtt tccaccacca
     3421 gtgaacgtca gggtcgcgtg attgaccgcg accaggcggg atccatggag ctgaaaaaac
     3481 gtcaggggta tttttaagat gaacaccgta cttgcacaac aaatcgccaa tgaaggcggc
     3541 gtcgaagcct ggatgattgc gcaacaacat aaaagcctgc tgcgttttct gacctgtggt
     3601 agcgtcgatg acggcaaaag taccctgatt ggtcgtctgc tgcacgatac ccgccaaatc
     3661 tacgaagatc agctctcatc gctgcataac gacagtaagc gtcacggaac ccagggcgaa
     3721 aagctggatc tggctctgct ggtggacggc ctgcaagctg agcgcgaaca gggcatcact
     3781 attgatgtgg cctaccgcta tttctctacc gagaagcgta aatttattat cgccgacacc
     3841 ccagggcacg agcagtacac tcgcaatatg gcgactggcg catcgacatg tgaactggcg
     3901 atcttactga ttgatgcccg taaaggcgtg ctcgatcaaa cccgtcgtca cagttttatc
     3961 tccacactgt tggggatcaa acatctggtc gtggcgatca acaaaatgga tctggtggat
     4021 tacagtgaag agacgttcac ccgtattcgt gaagattatc tgacctttgc cgggcagctg
     4081 ccgggtaatc tggatatccg ctttgtgccg ctctccgcac tggaaggtga caacgtggct
     4141 tcgcaaagtg aaagtatgcc gtggtacagc ggtctgacac tgctcgaagt gctggaaacc
     4201 gtggagatcc agcgagtggt ggatgcccag ccaatgcgct tcccggtgca gtacgttaac
     4261 cgcccaaatc tcgattttcg tggctacgcc ggaatgctgg catccggtcg cgtggaagtc
     4321 gggcaacgtg taaaagtgct gccctctggt gtggaatcaa acgtcgcgcg gatcgtgact
     4381 tttgatggtg atcgcgaaga agtctttgcc ggagaagcga tcaccctggt gctgacggat
     4441 gagatcgata tcagccgtgg tgatctgctg ctggcggcag acgaagcgtt accggcggtg
     4501 cagagtgcgt cggtggatgt ggtatggatg gcggaacagc cgctttcccc gggccagagt
     4561 tatgacatca aaattgccgg taagaagacg cgtgctcgtg ttgatggcat tcgttatcag
     4621 gttgatatta ataaccttac ccaacgcgaa gttgaaaacc tgccgctgaa cggcatcggc
     4681 ctggtggatc tcacttttga cgagccactg gtgttagatc gctatcagca aaatccggtt
     4741 accggtgggc tgatttttat cgatcgcctg agcaatgtga ccgtgggtgc cggtatggtg
     4801 cacgagccag ttagccaggc aacagctgcg tcatctgaat tcagtgcatt cgaactggaa
     4861 ttgaatgccc tggttcgtcg ccactttccg cactggggcg cgcgcgattt gctgggggat
     4921 aaataatggc gctgcatgac gaaaacgtcg tctggcatag tcatccggtc actgtgcaac
     4981 aacgcgaact acaccacggt catcgtggtg tagtgctgtg gtttaccggc ctctccgggt
     5041 ccggtaaatc aacggtcgcc ggggcgctgg aggaggcgtt acataaactc agcgtcagta
     5101 cgtatctgct ggatggcgac aatgttcgcc acggattatg cagcgatctc ggttttagcg
     5161 atgccgatcg taaagagaat atccgtcgcg tcggtgaagt ggcgaatttg atggttgaag
     5221 ccggactggt ggtgctgacc gcatttatct cgccacaccg cgccgaacgc cagatggttc
     5281 gcgaacgcgt aggagaaggg cgctttattg aagtgtttgt cgatacgccg ctggcgattt
     5341 gcgaagcccg cgatccaaaa ggcttatata agaaagcgcg tgccggtgaa ctgcgcaact
     5401 ttacgggaat agattccgtt tacgaagcgc ctgaatcggc agaaattcat ctcaatggtg
     5461 aacaattagt aacaaatttg gtacagcaat tattagatct gttgagacag aacgatatta
     5521 tcagatcctg agacaccacc gggtttccat gcccggttcg tcaaagtaac aggatcaaat
     5581 atgcgtaata gccataacat tacactaaca aataacgaca gccttactga ggatgaagaa
     5641 actacatggt cactgcctgg tgccgtggtc ggttttatct cctggttatt tgcgctggcg
     5701 atgccgatgt tgatttatgg ctctaacacg ctgttcttct ttctctacac ctggcctttc
     5761 tttctggcac tgatgcctgt cgcggtagtg gtggggattg cgctgcattc attgatggac
     5821 ggaaagctac gctacagtat tgttttcacc ctggtgacag taggcattat gtttggcgca
     5881 ctgtttatgt ggctactggg ctaatttgta ctttcttgtc tcctctgttc ataattcaaa
     5941 ccgtaactaa taatgagatt atgttctgca cgccctgggt atacgtaaca atgggcaaat
     6001 gtggtacatt tgcccgcgtt gtcgcggtat ccccaacaga ggatgtagag tcgtcttcgg
     6061 atgcatggga tgatgatgca gtttttcagg gggcaggatg ggtaaactaa cgctgctgtt
     6121 gctggctatt ctggtctggc tacagtattc gctgtggttc ggtaagaacg gtatacatga
     6181 ctatacccgc gtcaatgatg atgtggcggc actgcaagct acaaacgcga aacttaaagc
     6241 gcgaaacgat caactttttg ccgaaattga cgatctcaat ggcggccagg aggcgctcga
     6301 agagcgtgcg cgtaatgaac tcagcatgac caggccgggc gaaacttttt atcgtctggt
     6361 gcctgacgcg tcgaagcgcg cacagtctgc ggggcaaaac aatcgataaa tcagcccggg
     6421 aattaacatg gcaaccactc atttggatgt ttgcgccgtg gttccggcgg ccggatttgg
     6481 ccgtcgaatg caaacggaat gtcctaagca atatctctca atcggtaatc aaaccattct
     6541 tgaacactcg gtgcatgcgc tgctggcgca tccccgggtg acacgtgtcg tcattgccat
     6601 aagtcctggc gatagccgtt ttgcacaact tcctctggcg aatcatccgc aaatcaccgt
     6661 tgtagatggc ggtgatgagc gtgccgattc cgtgctggca ggtctgaaag ccgctggcga
     6721 tgtgcagtgg gtattggtgc atgacgccgc tcgtccttgt ttgcatcagg atgaccttgc
     6781 gcgattgttg gcgttgagcg aaaccagccg cacgggaggg atcctcgccg caccagtgcg
     6841 cgatactatg aaacgtgccg aaccgggcaa aaatgccatt gctcataccg ttgatcgcaa
     6901 cggcttatgg cacgcgctga cgccgcaatt tttccctcgt gagctgttac atgactgtct
     6961 gacgcgcgct ctaaatgaag gcgctaccat taccgacgaa gcctcggcgc tggaatattg
     7021 cggatttcat ccgcagttgg tcgaaggccg cgcggataac attaaagtca cgcgcccgga
     7081 agatttggca ctggccgagt tttacctcac ccgaaccatc catcaggaga atacataatg
     7141 cgaattggac acggttttga cgtacacgcc tttggcggtg aaggcccaat tatcattggt
     7201 ggcgtacgca ttccttacga aaaaggattg ctggcgcatt ctgatggcga cgtggcgctt
     7261 catgcgttga ccgatgcatt gcttggcgcg gcggcgctgg gggatatcgg caaactgttc
     7321 ccggataccg atccggcatt taaaggtgcc gacagccgcg agctgctacg cgaagcctgg
     7381 cgtcgtattc aggcgaaggg ttataccctt ggcaacgtcg atgtcactat catcgctcag
     7441 gcaccgaaga tgttgccgca cattccacaa atgcgcgtgt ttattgccga agatctcggc
     7501 tgccatatgg atgatgttaa cgtgaaagcc actactacgg aaaaacttgg atttaccgga
     7561 cgtggggaag ggattgcctg tgaagcggtg gcgctactca ttaaggcaac aaaatgattg
     7621 agtttgataa tctcacttac ctccacggta aaccgcaagg caccgggctg ctgaaagcca
     7681 atccggaaga ctttgtggtg gtggaagatt tgggctttga gcctgatggt gaaggtgagc
     7741 atattctggt tagaatcctc aaaaacggct gcaatacccg ttttgtggcg gatgcactgg
     7801 cgaaattcct gaaaattcat tcccgtgaag tcagcttcgc tgggcaaaaa gacaaacatg
     7861 ctgtaacgga acagtggtta tgtgctcgcg tgccgggcaa ggaaatgccc gatctgagcg
     7921 cctttcaact ggaaggctgc caggtgctgg agtatgcgcg gcacaagcgc aagctgcgtt
     7981 taggggcgct gaaaggtaac gcctttaccc tggttctgcg cgaagtgagc aatcgcgatg
     8041 acgttgaaca acgtctgatc gatatttgcg taaaaggtgt accgaactac ttcggtgccc
     8101 aacgttttgg gattggcggt agcaacttgc agggcgcgct gcgctgggcg caaaccaata
     8161 ctccggtgcg cgatcgcaat aaacggagtt tttggttgtc ggcagcccgc agtgcgttgt
     8221 ttaatcagat tgttgctgag cgcctcaaaa aagcagacgt taatcaagtt gttgacggcg
     8281 atgcgctaca attagccgga cgtggtagct ggtttgtagc aaccaccgaa gaactggcgg
     8341 aattacagcg tcgcgtcaac gataaagagc tgatgataac cgccgcattg ccgggcagtg
     8401 gcgaatgggg aactcagcgt gaagcgctgg cattcgaaca agcagctgtc gccgcagaaa
     8461 ctgaattaca agctttactg gtgcgcgaaa aagttgaagc cgcgcgcaga gcgatgctgc
     8521 tgtatccgca acaattaagc tggaactggt gggatgacgt caccgtagag atccgtttct
     8581 ggcttccggc gggtagtttt gcaaccagcg ttgtcaggga acttatcaac acaacaggtg
     8641 attatgcgca tattgctgag taattatgac ggggtacatg cacccggtat acaaacgctg
     8701 gcgaaagcct tgcgtgagtt tgctgacgtt caggtggtcg cccccgatcg taaccgcagc
     8761 ggcgcttcaa attctctgac actggaatcc tccctgcgca cgtttacctt tgaaaatggt
     8821 gatattgctg tgcaaatggg aaccccgacc gattgtgtct atcttggcgt gaatgctctg
     8881 atgcgtccgc gcccggacat tgttgtgtcc ggaattaacg ccgggccgaa tctgggggat
     8941 gatgttattt attccggtac ggtagccgcc gcgatggaag gccgtcattt aggttttccg
     9001 gcgctggccg tctcgcttga cgggcataaa cattacgaca ctgccgcggc ggtaacctgt
     9061 tcaattttgc gcgcactgtg taaagagccg ctgcgcaccg gacgtattct taatattaac
     9121 gttcaggatt tacccttgga tcaaatcaaa ggtattcgcg tgacgcgctg cggtacacga
     9181 catccggcag atcaggtgat cccgcagcaa gatccgcgcg gcaatacgct gtactggatt
     9241 ggcccgccgg gcggtaaatg tgatgccggt ccggggaccg attttgctgc ggtagatgag
     9301 ggctatgtct ccatcacgcc gctgcatgtg gatttaactg cgcatagcgc gcaagatgtg
     9361 gtttcagact ggttaaacag cgtgggagtt ggcacgcaat ggtaagcaga cgcgtacaag
     9421 cacttctgga tcaattacgt gcgcaaggta ttcaggatga gcaggtgctg aatgcacttg
     9481 ccgccgtgcc gcgtgaaaaa ttcgttgatg aagcgtttga acaaaaagcc tgggacaata
     9541 tcgccttgcc gataggtcag gggcagacaa tttcgcagcc gtatatggtg gcgcgaatga
     9601 ccgaattact cgagctgacg ccgcagtcgc gggtgctgga aattggcact ggttcgggat
     9661 atcaaacggc aatcctggcg catcttgtcc agcatgtttg ctcggttgaa cggattaaag
     9721 gcttgcagtg gcaggcacgt cgccgcctga aaaatcttga tttacataat gtttcaaccc
     9781 gtcatggtga tggatggcaa ggttggcagg cacgtgcgcc gtttgacgct atcattgtta
     9841 cggcggcacc gccggaaatt ccaactgcgc taatgacgca gctggacgaa ggcgggattc
     9901 tcgtcttacc cgtaggggag gagcaccagt atttgaaacg ggtgcgtcgt cggggaggcg
     9961 aatttattat cgataccgtg gaggccgtgc gctttgtccc tttagtgaag ggtgagctgg
    10021 cttaaaacgt gaggaaatac ctggattttt cctggttatt ttgccgtagg tcagcgtatc
    10081 gtgaacatct tttccagtgt tcagtagggt gccttgcacg gtaattatgt cactggttat
    10141 taaccaattt ttcctggggg ataaatgagc gcgggaagcc caaaattcac cgttcgccgc
    10201 attgcggctt tgtcactggt ttcgctatgg ctggcaggct gttctgacac ttcaaatcca
    10261 ccggcaccgg tcagctccgt taatggcaat gcgcctgcaa atactaattc tggtatgttg
    10321 attacgccgc cgccgaaaat ggggacgacg tctacagcgc agcaaccgca aattcagccg
    10381 gtacagcagc cacaaattca ggctactcaa caaccgcaaa tccagccagt gcagccagta
    10441 gctcagcagc cggtacagat ggaaaacgga cgcatcgtct ataaccgtca gtatgggaac
    10501 attccgaaag gcagttatag cggcagtacc tataccgtga aaaaaggcga cacacttttc
    10561 tatatcgcct ggattactgg caacgatttc cgtgaccttg ctcagcgcaa caatattcag
    10621 gcaccatacg cgctgaacgt tggtcagacc ttgcaggtgg gtaatgcttc cggtacgcca
    10681 atcactggcg gaaatgccat tacccaggcc gacgcagcag agcaaggagt tgtgatcaag
    10741 cctgcacaaa attccaccgt tgctgttgcg tcgcaaccga caattacgta ttctgagtct
    10801 tcgggtgaac agagtgctaa caaaatgttg ccgaacaaca agccaactgc gaccacggtc
    10861 acagcgcctg taacggtacc aacagcaagc acaaccgagc cgactgtcag cagtacatca
    10921 accagtacgc ctatctccac ctggcgctgg ccgactgagg gcaaagtgat cgaaaccttt
    10981 ggcgcttctg aggggggcaa caaggggatt gatatcgcag gcagcaaagg acaggcaatt
    11041 atcgcgaccg cagatggccg cgttgtttat gctggtaacg cgctgcgcgg ctacggtaat
    11101 ctgattatca tcaaacataa tgatgattac ctgagtgcct acgcccataa cgacacaatg
    11161 ctggtccggg aacaacaaga agtgaaggcg gggcaaaaaa tagcaaccat gggtagcacc
    11221 ggaaccagtt caacacgctt gcattttgaa attcgttaca aggggaaatc cgtaaacccg
    11281 ctgcgttatt tgccgcagcg ataaatcggc ggaaccaggc ttttgcttga atgttccgtc
    11341 aagggatcac gggtaggagc caccttatga gtcagaatac gctgaaagtt catgatttaa
    11401 atgaagatgc ggaatttgat gagaacggag ttgaggtttt tgacgaaaag gccttagtag
    11461 aagaggaacc cagtgataac gatttggccg aagaggaact gttatcgcag ggagccacac
    11521 agcgtgtgtt ggacgcgact cagctttacc ttggtgagat tggttattca ccactgttaa
    11581 cggccgaaga agaagtttat tttgcgcgtc gcgcactgcg tggagatgtc gcctctcgcc
    11641 gccggatgat cgagagtaac ttgcgtctgg tggtaaaaat tgcccgccgt tatggcaatc
    11701 gtggtctggc gttgctggac cttatcgaag agggcaactt ggggctgatc cgcgcggtag
    11761 agaagtttga cccggaacgt ggtttccgct tctcaacata cgcaacctgg tggattcgcc
    11821 agacgattga acgggcgatt atgaaccaaa cccgtactat tcgtttgccg attcacatcg
    11881 taaaggagct gaacgtttac ctgcgaaccg cacgtgagtt gtcccataag ctggaccatg
    11941 aaccaagtgc ggaagagatc gcagagcaac tggataagcc agttgatgac gtcagccgta
    12001 tgcttcgtct taacgagcgc attacctcgg tagacacccc gctgggtggt gattccgaaa
    12061 aagcgttgct ggacatcctg gccgatgaaa aagagaacgg tccggaagat accacgcaag
    12121 atgacgatat gaagcagagc atcgtcaaat ggctgttcga gctgaacgcc aaacagcgtg
    12181 aagtactggc acgtcgattc ggtttgctgg ggtatgaagc ggcaacactg gaagatgtag
    12241 gtcgtgaaat tggcctcacc cgtgaacgtg ttcgccagat tcaggttgaa ggcctgcgcc
    12301 gtttgcgcga aatcctgcaa acgcaggggc tgaatatcga agcgctgttc cgcaagtaag
    12361 taagcatctg tcagaaaggc cagtctcaag cgaggctggc ctttttcttt tgtttgggaa
    12421 gcttgtgttt ttgtcgcctg gataagacac gtcagcgtcc tacccggcaa tcagcattct
    12481 atgtgctcat catcttgcgc accaactgca taaacagctc ctgctcttct ccactcaaac
    12541 gccccaaaaa ttcctcatcc acgctgtcac caataggtat cgccgcagca agtagctttt
    12601 ctccttcggc agtcagccag acaaagcgtc gccgcttgtc agcgacgtca tgttcacgtc
    12661 tgactaagcc acgattttcc attcttgcca acatttctgc tagcgttgct ttggtgctga
    12721 ctgctgcttc tatcagtgcc acctgttcaa taccaggttt atcagcaatg gcgcgcatca
    12781 ccgcgtactg tggtttggtc aggtcaggta actcgtgctg ccagcgagcc gtgtgctgct
    12841 gaaaaagctg tcgtaactga tggaacgctt tatttcgtaa cgccatgtaa actccgggct
    12901 aattctctgt cgctatcata gcggtttttt agtgacgaag aagatgttgt tacttttcaa
    12961 tccttgccgc tgtggaaatg aaggagtatg ttaataataa cgttcgtata cgaataatta
    13021 agagaataag caatgaaact gatcgtcggg atgacagggg ctaccggtgc gcctcttggt
    13081 gtggcattac tgcaagcgct gcgtgagatg ccgaatgtcg agactcatct ggtgatgtcg
    13141 aagtgggcga aaaccaccat tgaactggaa acgccttaca gcgctcgcga tgttgctgcc
    13201 ctcgcagact tcagccataa cccggcggat caggcggcga tcatctcatc cggttctttt
    13261 cgtaccgacg gcatgatcgt tattccgtgc agtatgaaaa cgctcgccgg tatccgcgct
    13321 ggttacgccg atggcctggt agggcgcgcg gcggacgtcg tgctcaaaga aggccgcaaa
    13381 ctggtgctgg tgccgcgtga aatgccgctt agcaccatcc atctcgaaaa tatgctcgca
    13441 ctttcacgca tgggcgtggc gatggtgccg ccgatgcctg ccttttataa ccatcccgaa
    13501 acggtagatg acattgtcca ccatgtggta gcccgcgtgc tggatcaatt tggcctcgaa
    13561 catccccacg ccaggcgctg gcaaggattg ccgcaggccc ggaatttttc tcaggagaat
    13621 gaataatggc atttgatgat ttacgcagct ttttacaggc gcttgatgac cacggccagt
    13681 tactgaaaat cagcgaagaa gtgaacgctg agccggatct ggcagcagca gctaacgcca
    13741 ccgggcgtat cggcgacggc gcgcccgcgc tgtggtttga taatattcgc ggctttaccg
    13801 atgcccgcgt ggcgatgaat accatcggtt cctggcagaa ccacgcgatt tcccttggcc
    13861 tgccaccaaa caccccggtc aaaaagcaga ttgatgagtt tatccgccgc tgggataact
    13921 tcccgattgc cccggagcgc cgcgccaatc cagcctgggc gcagaacacc gtcgatggcg
    13981 aagagatcaa cctgttcgat attctgccgc tgtttcgcct gaatgatggc gatggcggtt
    14041 tctatctcga caaagcgtgc gtggtttccc gcgatccgct cgacccggat aacttcggca
    14101 agcagaacgt cggcatctac cgcatggaag tgaagggcaa gcgtaagctc ggcctgcaac
    14161 cggtgccgat gcacgatatc gccctgcatc tgcataaagc agaagagcgc ggtgaagatc
    14221 tgccgattgc gatcacgctc ggtaacgatc cgatcatcac gctgatgggg gccacgccgc
    14281 tgaaatacga tcagtctgag tatgaaatgg caggcgcgct gcgtgaaagc ccgtacccga
    14341 tcgccaccgc tccgctgacc ggttttgatg tgccgtgggg ttcagaagtg atcctcgaag
    14401 gggttatcga aagccgtaaa cgcgaaatcg aagggccgtt cggtgagttt accgggcact
    14461 actccggtgg gcgcaacatg accgtggtgc gcatcgataa agtctcttat cgcagcaaac
    14521 cgattttcga atcactctat ctcggtatgc cgtggaccga aatcgactac ctatgactag
    14581 gtaatttatt caagataacc gggaagagat taaaaaagac gggaatagat tggaatcaac
    14641 aagttaggaa ctcaaggttt tcaagataaa gcgagtccaa aaatgttaaa cggttcgata
    14701 tcaataaaac attcaagata actcacaggt tcttacaata gcctttaaag accgttcaaa
    14761 aacgattcag gtgtttatta tagcgctgat gggtgcaagc gccaaaacca atgtaatttt
    14821 agcacagcta ggcacttgca ctgggttatg cgctggctgc tttctgaggt agatttaagt
    14881 gatcgccctt agcttcggac aacgcgtaaa tctctctaac cttttcgtct ggcaaattac
    14941 caaggcttaa cgctatggct ctacgaactc caactagagc gtgcaactct tggatgtgct
    15001 catccagaag aatgagtaat tgctccgctc cttttctccc aagaacatca gctacttgtt
    15061 gtttttcttg tggtttaaga aatgacaaga acattagaat ttgagcatca acattatccg
    15121 cattctcttt tttgggttct ttggaatctt ttagttcaac atcaccctct cccgtcatga
    15181 gccattccaa ggtaacattt tcgctttctg ctatcttttt taactgatac gggcgtggta
    15241 tcggtgcgta atcccgcctg ttgtaataat tttttaaagt gccttcattg atcccccatg
    15301 cccgagcagc cgcatttctt gaagggtatc gttctactaa cctaaagatt ctttcgataa
    15361 tcggttcttt tccatcggca ggaaaagaac cttggggtgc ttttctcatt cttggttcct
    15421 ttctatctaa ttcattgata aataataaaa ttaaaaaata aaccataaaa acaccaaaga
    15481 accctttgca gggaatcttt ggtatcttaa tctttatcca tacggatatc cgaccggata
    15541 tccgcgcggg taattttgta ggttaaagga aagatgagac aaagagaaga tagtgaccgc
    15601 gattggcctt cgctgaagat tcagctggcg ctcaaagata acgggtggtc ttccttccgg
    15661 gagctttcac tccatgctgg ttatgctcag gattcactga aagcggtagt tcgcacacca
    15721 aacaaaaaat acgaacgcgt aatttctgac gctataggtg taccgccaga aatcatctgg
    15781 cccagccggt atcgtaacga tggatttatc aggaaggcgt cgtgatatgc agtcgtacgt
    15841 taccgtaaat gacctgctcg gcgtaccagg aatgccagcc accacaaaag gcatccgaca
    15901 ggcgttacag cgtttttctc gtgatcttgg tgacgttagt cgccgtcggg aaggcacaaa
    15961 agccattgag taccacattg attgtctgcc tgaaataaca cgaaaggcac tgcgtgaacg
    16021 ctatgtggaa cagctggtgg ctacagagaa caatgtttct gaagtgaaag ctgttaccag
    16081 aaaaacacgc aatcctgacg ctgtccaggc aatcgaagca tatcgcggtt ctccacaact
    16141 gatggaagaa cgcctgaatg cgctgaccga aaaccagcgc tgggtatctg aagcaagagc
    16201 tgcgctggtg gtggaagtgc tgaagctgga aagcgccggt aaccccgggc gactgaaagc
    16261 cattaacttt cttgttgaaa aagcccgtaa aggtgagctg ccggagcgcc tgcaacaggc
    16321 cgcagttaac gccaatgcaa aacgtggcgc taatcgtact atcagccgcg atccacttta
    16381 ccagtgggtt ctgaaataca accagtcgca gaacgccgcc gaacggctgc tgttactggc
    16441 accgggaaaa cgcgacgaaa taaaaccgga agaaatcagc tggctgcctg aatttctggc
    16501 gcagtatcgc caggtgaacg gcagacccat gtcagaagcc tacgaggatt ttgtcgcaga
    16561 atggcagcgt cgccatgcgg acgaaccgta catgctggag gtgatgcctt cctatgatgt
    16621 tgtccggtat gccatgaaaa aactgccgga agtggtgaaa caaaaagggc gcgttaccgg
    16681 cagtgaatac cgtcagcttg aaggattcac ccgccgcgac tggacagcta tgccggtgaa
    16741 ttatgtctgg attggtgacg gtcacggtat gaagctgaaa tgtgcgcatc cgatccacgg
    16801 gcgtccattc tcaccggaag tcacgtttgt gatcgatggt ggaacacggt ttgttgttgg
    16861 ctggagtctt gaccttgccg aaaacgtgtt cgccgttgcc ggtgcgatac agcacggcat
    16921 tcgcaaccac ggaaaaccgt tcctgtatta ctcggataac ggttccggtg aaacggctga
    16981 catgctggac aaagaggtcg tgggtattct gccacgcctg gggattaaac accctaccgg
    17041 tattgccggt aatccacagg gacgcggcat tattgaacgc ctgaaccgga cactgccgat
    17101 gcgtatagcc aggaggtatc gcacctattt tggcaagggt gctgaccggg aatcgttgcg
    17161 tgtacttaac cgtgatctgc gttcagcgtt taacgccctg caacaggaca agcctctgaa
    17221 cgaccgccag aaagcggcaa tgcgcgagct gccatcgtgg gcggagctta ttgaggctat
    17281 tcgtgaaggc gtggaatggt acaacaaccg cccgcattct gagctgccga tgaaaccgaa
    17341 cggacggcat tacagcccga cagagttcag aaaaaaacgc caggcagaag aagacaccga
    17401 aattgagtgg ctgagtgatc ttgagctgcg cgacatgttc cgcccaatgg tggaacgacc
    17461 ggtaagacgc tgcgaaattc agtggctgaa caatatttac tacgcgccgg agttgcgtga
    17521 cgaacacggt cgcaaagtgc ttatcagtta tgacattcat gacgccgagc gaattacggt
    17581 acgtcgcaag gacggcagct ttatctgtga ggcgatatgg aacggcaata aacgcgcagc
    17641 ctttgctgtc agtgctgaat atcacaaaca acagcagcgt attaaaggaa tgcgcaaacg
    17701 cgctgaggaa aaaattcgtg atgctgaaga tgaaggtatt cagattctgg aacacaaaca
    17761 ggctgaaccc tggctgagta atgtttatcg cccggttggt aatgtggtgg cagttcagca
    17821 accggaatat gaagaagagc atgacgaaga atttgaacgc gatttccggc tggggatgca
    17881 aaaacttttt gccatgcagg aagaagatga tccgctggcc tgaataaaaa aacagtccgc
    17941 gggaacggac tgttaaataa aggaaacaaa gctgatttga aataaccgag gaaatactat
    18001 gactgatatt gatgatgttt gaggtgtact ggcaatagcg gacactacca tttgttcttt
    18061 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat a
//
LOCUS       DBBBSB010000083        17960 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.083, whole genome shotgun sequence.
ACCESSION   DBBBSB010000083 DBBBSB010000000
VERSION     DBBBSB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.083"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            148..672
                     /locus_tag="ACIUS0_002785"
     CDS             148..672
                     /locus_tag="ACIUS0_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HGB0220473.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            753..1427
                     /locus_tag="ACIUS0_002786"
     CDS             753..1427
                     /locus_tag="ACIUS0_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGB0220474.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1443..3905
                     /locus_tag="ACIUS0_002787"
     CDS             1443..3905
                     /locus_tag="ACIUS0_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGB0220475.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3921..4954
                     /locus_tag="ACIUS0_002788"
                     /pseudo
     CDS             3921..4954
                     /locus_tag="ACIUS0_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5036..5374)
                     /gene="rcnB"
                     /locus_tag="ACIUS0_002789"
     CDS             complement(5036..5374)
                     /gene="rcnB"
                     /locus_tag="ACIUS0_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGB0220476.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5593..6423)
                     /gene="rcnA"
                     /locus_tag="ACIUS0_002790"
                     /pseudo
     CDS             complement(5593..6423)
                     /gene="rcnA"
                     /locus_tag="ACIUS0_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6545..6817
                     /gene="rcnR"
                     /locus_tag="ACIUS0_002791"
     CDS             6545..6817
                     /gene="rcnR"
                     /locus_tag="ACIUS0_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGB0220477.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6909..7005
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7040..7828
                     /gene="thiM"
                     /locus_tag="ACIUS0_002792"
     CDS             7040..7828
                     /gene="thiM"
                     /locus_tag="ACIUS0_002792"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGB0220478.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7825..8625
                     /gene="thiD"
                     /locus_tag="ACIUS0_002793"
     CDS             7825..8625
                     /gene="thiD"
                     /locus_tag="ACIUS0_002793"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGB0220479.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8690..9507
                     /locus_tag="ACIUS0_002794"
                     /pseudo
     CDS             8690..9507
                     /locus_tag="ACIUS0_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            9559..10305
                     /locus_tag="ACIUS0_002795"
     CDS             9559..10305
                     /locus_tag="ACIUS0_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGB0220480.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10279..11244)
                     /locus_tag="ACIUS0_002796"
     CDS             complement(10279..11244)
                     /locus_tag="ACIUS0_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGB0220481.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11241..12244)
                     /locus_tag="ACIUS0_002797"
                     /pseudo
     CDS             complement(11241..12244)
                     /locus_tag="ACIUS0_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12284..13336
                     /gene="fbaB"
                     /locus_tag="ACIUS0_002798"
     CDS             12284..13336
                     /gene="fbaB"
                     /locus_tag="ACIUS0_002798"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGB0220482.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13644..14498
                     /gene="gatY"
                     /locus_tag="ACIUS0_002799"
     CDS             13644..14498
                     /gene="gatY"
                     /locus_tag="ACIUS0_002799"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGB0220483.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14527..15789
                     /gene="gatZ"
                     /locus_tag="ACIUS0_002800"
     CDS             14527..15789
                     /gene="gatZ"
                     /locus_tag="ACIUS0_002800"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HGB0220484.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15799..16251
                     /gene="gatA"
                     /locus_tag="ACIUS0_002801"
     CDS             15799..16251
                     /gene="gatA"
                     /locus_tag="ACIUS0_002801"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGB0220485.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16282..16566
                     /gene="gatB"
                     /locus_tag="ACIUS0_002802"
     CDS             16282..16566
                     /gene="gatB"
                     /locus_tag="ACIUS0_002802"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGB0220486.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16570..17925
                     /locus_tag="ACIUS0_002803"
     CDS             16570..17925
                     /locus_tag="ACIUS0_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGB0220487.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4684 a         4101 c         4564 g         4611 t
ORIGIN      
        1 tgttgtattt atgttgcatt ggttaatttg ttttaaatca ataaaacaat atgtttacat
       61 tgcggcagct tttttgatgt gagaaatttg tttccaagag atacagactc ttaacaacca
      121 aaaataaatt ttattaagga acatattatg aaacgttcaa ttattgctgc cgctgtcttt
      181 tcttcttttt ttatgagcgc tggagtattt gctgcagacg ttgataccgg aacattaacc
      241 attaaaggaa atattgcaga atctccgtgt aaattcgaag cgggtggtga ttcagtaagt
      301 attaatatgc cgactgtacc aaccactgtc tttgaaggta aagctaaata ttctacctat
      361 gatgatgcag tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa agaagttgct
      421 ggtgtaaaac tctcgttgat cacaaacgac aaaataaccg gtaacgataa ggcgatagcc
      481 agtagcaacg ataccgtagg tgataacagc gatgtcctag atgtttctgc accttttaac
      541 attgagagtt ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg
      601 aaactgacag ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg
      661 gcgcaggatt aataaataaa attgcaagtt tctccaggta ataaacatgg ccgctatccc
      721 catggcggct ttttaattta agaggcatta agatgaaagg attattatct ttactcattt
      781 tttctatggt ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc
      841 cggcagaaaa taaagaagtt atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc
      901 tgcaggcgtg gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt
      961 tcatgttaac gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca
     1021 aaattatgcc gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgt
     1081 atattccacc aaatagtcca gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa
     1141 acagaattaa gttgttttac cggccagcgg gtattgctcc ggtaaataaa gcgaccttta
     1201 aaaagttact ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt
     1261 gggtgacgat ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga
     1321 ttgccccctt agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc
     1381 tgaccattat caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc
     1441 ctatgttgag aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag
     1501 cttatgcagc tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc
     1561 aggttgcaaa tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata
     1621 tttatgtcaa taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag
     1681 aaacatgttt atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca
     1741 gcggtaagca atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata
     1801 tcggggtttt tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg
     1861 gctatgttcc accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg
     1921 tgagtcagta ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt
     1981 ttaacagcgg gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa
     2041 caaataacaa tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc
     2101 aatttctcgg cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg
     2161 ttcgcttcag cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa
     2221 attttacgcc acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga
     2281 atggttttgt ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc
     2341 agttggccgg tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa
     2401 ccacctatct ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat
     2461 atgattttgc ggcgggggcg agcaagcaaa gtgattttgt ccaggcaggt tatcagtatg
     2521 gttttaataa cttattgacg ctgtatggcg gcacgatggt tgctaataat tactatgcgt
     2581 ttaccctcgg aacgggttgg aacacgcgca ttggcgcaat ttccgtcgat gccacgaagt
     2641 cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca aagttatcaa attgcctaca
     2701 acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc
     2761 gtgattaccg gacatttaac gatcacgttt gggcaaacaa taaagataat tatcgccgtg
     2821 atgaaaacga tatctatgac attgccgatt attaccagaa cgattttggc cgtaaaaata
     2881 gcttttctgc caatatgagc cagtcattgc cagaaggctg gggttctgtg tccttaagta
     2941 cgttatggcg agattactgg gggcgtagcg gcagcagtaa ggattatcag ttgagttatt
     3001 ccaataactg gcggcggata agctataccc tcgcggcaag ccaggcgtat ggcgagaatc
     3061 atcatgaaga gaaacgtttt aatattttta tatcaattcc ctgtgattgg ggtgatgacg
     3121 ttacgacgcc tcgtcggcaa atatatatgt ctaactcaac gacgtttgat gatcagggtt
     3181 ttgcctcaaa taatacggga ttatcaggaa cagtagggag tcgggatcag ttcaattatg
     3241 gtgtcaacct gagtcatcaa catcagggaa atgaaacgac agctggggcg aatttgacct
     3301 ggaacgcgcc ggttgcgaca gtgaatggca gttatagtca gtcgagtact tatcgacaga
     3361 ctggagccag tgtttcaggg ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc
     3421 gtctttccga aacgtttgct gtgatgaatg cgccaggaat taaagatgct tatgtcaatg
     3481 ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt atacgacgga atgacacctt
     3541 atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg
     3601 gcaaccggaa aattgccgcc ccttatcgcg gcgcggttgt actggttaat tttgataccg
     3661 atcagcgcaa gccctggttt ataaaagcgt taagagcgga tggtcaacca ttaacgtttg
     3721 gttatgaagt caatgatatc catggtcata atattggcgt tgtcggccag ggaagccagt
     3781 tatttattcg caccaatgaa ataccgccat cggttaatgt agcaattgat aagcaacaag
     3841 gactttcatg cacaatcacc ttcggtaaag agattgatga aagtagaaat tatatttgcc
     3901 agtaatagtc aggtggttct atggaaattc gcataatgct atttatatta atgatgatgg
     3961 ttatgcctgt gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg
     4021 ttcaggggaa ttttgcactt actcaaacac aaatagcgac atatgagcat aattttaatg
     4081 attcgtcatg cgtgagtaca aatactatca cccctatgag cccatcggat attattgttg
     4141 gactttataa cgataccatt aaattaaatt tacattttga atggaccaat aaaaacaaca
     4201 tcacgttgtc aaataatcag agcagtttca ccagtggtta ttcggtaacg gtgacacctg
     4261 cggccagtaa tgcaaaagtg aatatttctg cggggggcgg tggttcagtg atgattaatg
     4321 gtgttgcgac attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc
     4381 tactgtgttt attaggtggc aagtcatggg gtgcatgtgt aaatagctac agaaatgcat
     4441 tggcacaaaa tgcaggtgtc tattccttca atctgacatt gtcatacaac ccgataacca
     4501 caacctgcaa acaggacgat ttattaatta ctttagacag tattcccgtt ttacaattac
     4561 cagccacagg taacaaagca acaataaata gtaaaaaaag ggatattatt ctgcgttgta
     4621 aaaatttatt aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt
     4681 ctgacttgtt aaccaacagc aacacaatac tgaaaggtgc ggaagataat gcgtaggatt
     4741 tattcttgaa agtaatggtt cgccagtcac acttttaaat atcactaaca gcagtaaagg
     4801 atacacaaat ttaaaagaaa ttgcggcaaa gtcaaaactt acagatacaa cggtttcaat
     4861 tccgataaca gccagttact acgtctacga tacaaacaaa gttaaatctg gcgcactgga
     4921 ggcaaccgca ttaatcaacg tgaaatacga ctaataacaa gcaaaacgag caagtggcta
     4981 ataataaaaa ataacaaggt gtctgcattt ccccctcatg atgagggggc tttttttagc
     5041 gatgataaaa aatctcaccg tcgtaggctt taatgatttt accgtcggtg tcgctgatca
     5101 acacgtacgc gccacccata taggtccagt gcgtcccggc atcaggcgcg ggcaggttac
     5161 gtaactgcca ctgcttaatg ttgtactcat cggtgcggta catctctggg acggtatcac
     5221 ctggtttgaa atgcttaaag tcagcaatga aactacttaa ctcatactta gaaattcccg
     5281 aggtattggt cgaacccgcg gtggctggtg cggcccaggc ggcactggta accagcaaaa
     5341 gcacacccag caacatctta tttttaatgg tcataaactc tccactttgg ggatctgtaa
     5401 tatcagtcgc atatctttcc tgtcatacaa tataactgac aagcgatatt ttgttagtga
     5461 tcccatgatt tcgtaaaaag tgtaaatgaa aggatgccct aaaagcatcc ttagcgagca
     5521 gagagtcagc ggaaatagct gatttaagaa tcatcctggg ctgaagataa catgcaagcc
     5581 taaaggattg agttatcgca ttatgcccat gaagccatgt acgcccatat acacaccgac
     5641 taagccaatc aacagactgg aaaaataggg ggcgcgttta gcgagagtgt taaatccgct
     5701 ccagcgtttt gcgacctgct gaacgctgat tgctgcgcca acgcctacgg tgacaagcgt
     5761 taacgccagg ccaatgctga aactgacgac cagtgttgcg cccagtgtca gggctttcaa
     5821 ctgaatgcat atcaacagca cggtaattgc tgccgggcag gggataaagc caccggttaa
     5881 gccaaataac aaaatttgcc agttgatgac ctctctacca tcaaagcgtc gtttaatgtc
     5941 attggcatgg gctcgtgcat gggcatcctg atactcgcca tgttcgtgat ggtgatgatg
     6001 tccttggtcg tggtgatgtt cgtgatcgtg atgatggtga tgatgctcat aatcatgccc
     6061 gtgcatattc tccagccagt tgcgttcgcc gcgctaggta cgccagaaca tccagaacgc
     6121 ggtgccaata atgatcactg cggaaatcag ctggagccac ggttctgctg attgagcagt
     6181 aaagcgctta ctgatcacca tcccgccaaa ggcaatcaac cagaccaccg cggtatgcga
     6241 aatggttgct gccagtccga gcatcaccgc ctgtttaatg gtgcctttga tggcgatgat
     6301 aaacgccgcc atcatcgttt ttgagtgccc tggttccagg ccatgaagcg caccaagtaa
     6361 gatggcgctg gggatgaaga accaggcgtt tccttgctga agaagagttg taaattcggt
     6421 catgataata attcttagta ttaattcggc aatctgattc tactcccccc cagtacctga
     6481 tactaccccc ccagtagatt aatagtgcta tgatttttca tattcttgtt aaccaggtgt
     6541 tgccatgtct catacaatcc gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca
     6601 gggccaggtc gtggcgctca agaaaatgct cgacgagccg cacgaatgcg ctgcagtttt
     6661 acaacagatt gctgctatcc gtggagcggt aaacggtctg atgcgggaag tgattaaagg
     6721 tcatctgacg gaacacatcg ttcaccaggg ggatgagcta aaacgtgaag aagatctgga
     6781 tgtcgttctg aaggtgctgg attcatatat caaataattt attaacgcga ttgtaaaact
     6841 gccgtttttc ctcgtttaca acgggggcgc tggacattac catcctcctc tgcgatttat
     6901 catcgcaacc aaacgactcg gggtgccctt ctgcgtgaag gctgagaaat acccgtatca
     6961 cctgatctgg ataatgccag cgtagggaag tcacggacca ccaggtcatt gcttcttcac
     7021 gttatggcag gagcaaacta tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc
     7081 gttacacctt tttcaccaac attcccctct tgtgcactgc atgaccaatg atgtggtgca
     7141 aacctttacc gccaatacct tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac
     7201 cgaagaggcc agtcagtttg cggctatcgc cagtgccttg ttgattaacg ttggcacact
     7261 gacgcagcca cgcgcacagg cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac
     7321 acgctggacg cttgatccag tagcggttgg tgcgctcgat tatcgccgcc atttttgtca
     7381 tgaactttta tcttttaaac cggcagcgat acgtggtaat gcttcggaaa tcatggcatt
     7441 agctggcatt gctaatggcg gacggggagt ggataccact gacgccgcag ctaacgcgat
     7501 acccgctgca caaacactgg cacgggaaac tggcgcaatc gtcgtggtca ctggcgaggt
     7561 ggattatgtt accgatggac atcgtatcat tggtattcac ggtggcgatc cgttaatgac
     7621 caaagtggta ggaactggct gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc
     7681 aggcgatacg ctggaaaatg tcgcatctgc ctgtcactgg atgaaacaag ccggagaacg
     7741 cgcagtcgcc agaagcgagg ggccaggcag ttttgttcca catttccttg atgcgctctg
     7801 gcaattgacg caggaggtgc aggcatgaaa cgaattaacg ctctgacgat tgccggtact
     7861 gatccgagtg gtggtgcggg gattcaggcc gatcttaaaa ccttctcggc acttggcgct
     7921 tatggttgct cagttattac tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg
     7981 tatcgcattg agcctgattt tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga
     8041 atcgatacca ctaaaatcgg tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa
     8101 cggttgcaac gttatcagat ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc
     8161 ggcgacccgc tgctttcacc ttcggcggtt gctacgctgc gcagtcgatt attgccacag
     8221 gtttcattaa taacgccaaa cttgcccgaa gctgccgcct tgctcgacgc gccacacgcg
     8281 cgcaccgaac aggaaatgct ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca
     8341 gtgctaatga aaggtggtca tctggatgat gagcaaagcc cggactggct gtttacccgc
     8401 gagggtgaac aacggtttac cgcaccgcgc attatgacca aaaacaccca cggcactggt
     8461 tgtacactct ctgcggcgtt ggctgcacta cgcccgcgcc atacaaactg ggctgacacc
     8521 gtacaggagg caaaaatctg gctttcatcg gcgttagccc aggccgacac gctggaagtt
     8581 ggtcacggta ttggtccggt tcaccacttc cacgcctggt ggtgagcacc gtactatagc
     8641 tgctttggtt attgtcagcc tttggcactg acattcaggg atgaaaccaa tgcagctaag
     8701 aattaccagt cgaaaaaaac tcaccgctct attatgcgct cttgggctaa tttccatcgt
     8761 tgcaatttat ccgcgtcaga cggtgaattt tttctattcg acagcagttc agattacaga
     8821 ctacatccac ttctacggtt atcgcccggt taaatctttc gctattcgca ttcctgccag
     8881 ttacaccatt cacggattag atgtttcgcg ctggcaggag cgtatcgact ggcagcgggt
     8941 ggcaaaaatg cgcgacaacg atatccgctt acagtttgct tttattaagg ctacggaagg
     9001 cgaaaagctg gtggacccct atttttcgcg taactggcaa ttaagccgcg aaaatggcct
     9061 gctgcgcggg gcgtatcatt atttctcccc gtcggtatcc gcttcagttc aggcgcgatt
     9121 atttctgcaa acggtggatt tctcacaagg cgatttccct gccgtgctgg acgtagaaga
     9181 acggggaaaa ttatcggcaa aagaattacg caagcgggta agtcagtggc taaaaatggt
     9241 cgaaaaacgc acgggaaaaa agccgattat ttactcagga gccgtttttt atcacaccaa
     9301 tctggcgggc tatttcaatg aatatccgtg gtgggtggct cactattatc aacgtcgtcc
     9361 ggacaatgac ggcatggcct ggcgcttctg gcagcattcc gaccgtggac aggtagatgg
     9421 catcaatggt ccggtggatt ttaatgtgtt taatggcacg gggatgagtt gcaggcattc
     9481 gttgatggga ttaaagaaac gccttaaata agaagcagtg atatactccc accagactcc
     9541 ctggcaggaa aaagacaaat ggaacaagcg catacccagc tgattgccca actgaacgag
     9601 cgtattttag cggcggataa cacgccgctt tatattaagt ttgccgaaac ggtaaaaaat
     9661 gccgtgcgca gcggggtgct ggagcatggc aatattttgc ccggtgagcg tgatttaagt
     9721 cagttaaccg gcgtttcgcg cattacggtg cgcaaggcga tgcaggcgct ggaagaagag
     9781 ggcgtggtga cgcgttcgcg cggttacggc acgcagatca acaacatctt cgaatactcg
     9841 ttgaaagaag cgagaggttt ttctcagcag gtggtattgc gcgggaaaaa gcccgatacg
     9901 ctatgggtta ataagcgtgt cgtgaaatgt cccgaagaag ttgcgcagca gctggcggcc
     9961 gaagcgggaa gtgatgtctt tttgcttaag cgtattcgct atgtcgatga agaagcggta
    10021 tcgattgagg aatcgtgggt tccggcgcat ttaattcatg atgttgatgc catcggaatt
    10081 tctctttatg actatttccg cagccagcac atttacccac aacgtacgcg ttcccgcgtt
    10141 agcgcccgga tgccggatgc cgagtttcag tcacatattc agttagatag caaaataccg
    10201 gtgctggtga tcaagcaagt ggcgcttgac caacagcaac ggcctattga gtacagcatt
    10261 agccactgtc gcagcgatct atacgttttt gtgtgcgagg agtagttcct cgcgcgttgg
    10321 cgcacaatca ccgccccgat gcccgacaac ccacgacgcc actgcattgc ccagtaatac
    10381 ggcatccgcc agtggcagac cagaggccag cccggcaagt acgccacctg catgactgtc
    10441 gcccgcccca atggtgtcta caacttgcgt tggaaatgcc ggaatgcagc cagaagcgtc
    10501 gttgctgaaa taccatgcgc cttctttatc gagacgaacg atcaacggcg cggcaaattt
    10561 ctcctgccat tgcttgccaa gtgttgttat ctctgcggat aaagcaaaac gttcggcggc
    10621 aatctcagcc tcttgacgat tgagtgacac taaaggtcga caggccatga tccgcgccag
    10681 taatgcgtcc gggatatcgc caatacgtgg gcaaaaatcg ataaacggcg tcacgtcttg
    10741 cagcttttcc agccattcca ctaacaattc gccgcagggc gaggccagtt gataaccgga
    10801 aaaatagagc aggttgccag gcgcaacggt taatcgtgcc aaccattggc gattccattg
    10861 attttccaca ccgctgaatg acataaaagt gcgttcgcca tccggctcaa ccagtgccag
    10921 acaccagccg ttatcgcttt cggcgttatc gatcaggctg attaacccct ctttcgccat
    10981 ccggttgcga ataatctccg cccacacgcc ctgaccgagc ggcaaggcat tacccgcttc
    11041 gatgccgagg cgctttaacg ccacggcaat attcagtgcg cagccgccaa cgttaacgct
    11101 ctgctgtttc agttcgatat cgcacccacg ccaggggagg gcataagcgt cggcgatcac
    11161 gtcaatgacc gccgcgccga cgaccatcac cggctgacgc gtggttaatt caggcagcag
    11221 tgtgtgtaat cgagcgccgc tcataccgcc tcccgttgtt gacgatattt cgccagcgct
    11281 gtggcatagc ggttgaaatc aagctgattt accgcatcca gctccgcctt taacgcagga
    11341 tcgacagcgt taatgccatg caacgcgccg caaatcgccg tcgccatagc acctatggtg
    11401 tctgtgtcgc cgccaagatt ggcgcacagg acggcgcagc ggtttgggtc ggtttgtgcc
    11461 agttcaacta gcgcaatggc gcacggaacg gactcaatag tgctggtgcc tgcgccaacg
    11521 acctggtaaa gctgttcgct ggcggattcg gtgccgtcgg cattacgcac aattttcagc
    11581 gcaatctcca gacgtgccgc cagtgaggcg ctgaacgtgg tggtgcgttt ttgctgtgca
    11641 tgtcgcgcaa ttgaaggcaa tgaatcaaca atcgctgacc agcgttctcc gtcaatggca
    11701 cgagaaatcg cccatgcgat gactaccgcg cctgcaaccg ccagatcgga tttatgggtc
    11761 gggctggacg ccagcgccac atcatcaata aaggaatcaa catcacgcgc tggaagcaaa
    11821 caacctaatg gcgagacgcg catcgctgcg ccgttggtta cgccgttgtt ttccagttca
    11881 gcaacgggtt taccgtcgcg aatggcgtta agcgcaatct tcgaggtcgg acctagtacg
    11941 tttttgttaa aggcgtcgaa acgcaacgcc cagtcgagaa tattacgccc aatcagattc
    12001 ggatcgatct tgccttcaca ttccagtaac gcatccgcca gacacaggcc atcgaggtat
    12061 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggca
    12121 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    12181 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    12241 tcatttaact tccccttgaa tcggaattta cgggagcata gtaatgacag atattgcgca
    12301 gttgcttggc aaagacgccg acaacctttt acagcaccgt tgtatgacta ttccttctga
    12361 ccagctttat ctccccggac atgactacgt agaccgcgtg atgattgaca ataatcgccc
    12421 gccagcggtg ttacgtaata tgcagacgtt gtacaacacc gggcgtctgg ctggcacagg
    12481 atatctttct attctgccgg ttgaccaggg cgttgagcac tctgccggag cttcatttgc
    12541 tgctaacccg ctctactttg acccgaaaaa cattgttgaa ctggcgatcg aagcgggctg
    12601 taactgtgtg gcatcaactt acggcgtgtt ggtgtcggta tcgcggcgct atgcgcatcg
    12661 cattccattt ctcgtcaaac ttaatcacaa cgagacgcta agttacccga atacctacga
    12721 tcaaacgctg tatgccagcg tggagcaggc cttcaacatg ggcgcggtgg cggttggtgc
    12781 gactatctat tttggctcgg aagagtcacg tcgccagatt gaagaaattt ctgcggcctt
    12841 tgaacgcgcg cacgagctgg gcatggtgac agtgctgtgg gcctatttgc gaaactccgc
    12901 ctttaagaaa gatggcgttg attaccatgt ttccgccgac ctgactggtc aggcgaacca
    12961 tctggcggca accataggtg cagatatcgt caaacaaaaa atggcggaaa ataacggcgg
    13021 ctataaagcg attaattacg gttataccga cgatcgcgtg tacagcaagt taaccagcga
    13081 aaacccgatt gatctggtgc gttatcagtt agctaactgc tatatgggcc gggctgggtt
    13141 gataaactcc ggcggtgctg caggcggtga aactgacctc agcgatgcag tgcgtactgc
    13201 ggttatcaac aaacgcgcag gcggaatggg gctgattctt ggacgtaagg cgttcaagaa
    13261 atcgatggct gacggcgtga aactgattaa tgccgtgcag gacgtttatc tcgatagcaa
    13321 aattactatt gcctgatacg cctcatacga acagcaccac cccgtaggac ggataagacg
    13381 tttacactgc atccggcaac ctatgcctga tgcgacgcgg aagcgtctta tcatgcctac
    13441 atagcactgc cacgtatgtt tacaccgcat ccggcataaa aacacgcgca ctttgctacg
    13501 gcttccctat cgggaggccg tttttttgcc tttcactcct cgaacaattt tcatattgtt
    13561 gtttttgcga tcattatctc gacatttaaa agcaaataat ttcattatat tttgaaatcg
    13621 aaagcaaacg acaagatatg aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg
    13681 cacagcgcgg cggttatgcg gttccggcat tcaatattca caatctcgaa acgatgcaag
    13741 tggtggtgga aaccgctgcc aacctgcatg cgccggtcat catcgccgga acgcctggca
    13801 catttactca tgctggtaca gaaaatctgt tggcgctggt cagcgcgatg gcgaagcaat
    13861 atcaccatcc actggcaatt catctcgacc atcacacgaa atttgacgat atcgctcaga
    13921 aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa
    13981 atatttcacg ggtcaaagag gtggtggatt tttgccatcg ctttgatgtc agcgtcgagg
    14041 cggagctggg gcaacttggc ggccaggaag atgatgtgca agtcaatgaa gtcgatgcgt
    14101 tgtacaccaa ccccgcacag gcgcgtgaat ttgccgaggc aaccgggatt gattccctgg
    14161 cggtcgccat cggcacggct cacggtatgt atgccagcgc accggtgctt gatttttctc
    14221 gactggagaa cattcgccag tgggtgaact taccgctggt gctgcatggc gcgtcaggat
    14281 tatcgactaa ggatattcag caaaccatca aactggggat atgcaaaatc aacgttgcaa
    14341 cggagctgaa aaatgccttc ttgcagtcgt taaaaaatta cctgaccgag caccccgaag
    14401 cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca
    14461 aagtgattgc cgattgtggc tgcgagggca gggcataacg cacctgccat ttaacaagga
    14521 aaaaacatga aaacgttaat tgcccggcat aaagctggtg aacatatcgg catatgttca
    14581 gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg catttgatcg caacagcacg
    14641 cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc aatttggcgg ttataccgga
    14701 atgacaccgg cagactttcg cgaatttgtt tttacgattg ccgataaagt tgggtttgca
    14761 cgcgaacgca ttattctcgg cggcgatcat ctgggaccaa actgctggca gcaagaaaat
    14821 gcggatgcgg cgatggaaaa atccgtcgag ctggtaaaag catatgttcg tgctggcttc
    14881 agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg ataccatacc gttagcacca
    14941 gaaacggttg cggaacgagc tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat
    15001 tgccagcgtg agcaactgag ctatgtcatt ggcactgaag ttccggttcc gggcggtgag
    15061 gccagcgcca ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt
    15121 acgcatcaaa aggcctttat tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc
    15181 atcgtggtgc agccgggtgt ggaatttgat cacagcaata ttatccatta tcagccgcag
    15241 gaagcgcagg cactggcgca atggatagaa aacacccgaa tggtttatga agcacattct
    15301 accgattacc agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg
    15361 aaagtcggtc ccgcattaac ctttgcttta cgcgaggcga tatttgcact ggcacaaatt
    15421 gagcaggaac ttatcgcccc cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg
    15481 atgctcgacg aaccgcaata ctggaaaaaa tattatcgca cgggttttaa cgattcatta
    15541 ctggatattc gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt
    15601 aaaaatagcg ttgaaacgat gatggtgaat ctgcaaggcg tggacatccc actgggcatg
    15661 attagtcagt atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaatg
    15721 ccgcatcagc ttattatgaa taaaatttat gatgttttgc gcgcctatcg ctacggctgt
    15781 gcggaataag gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat
    15841 cgtagcgaag ttttaaccta tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat
    15901 acctggccac aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag
    15961 cagcacgcca ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat
    16021 ctgttaaggc caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggcg
    16081 gtatcgttgg ttattgcgtt gattgtggaa aatccgcagc agcaattgaa acttttacgc
    16141 tgtttatttg gcaagttaca acagcccgat atcgtcgaga cactaattac tcttcctgaa
    16201 actcagttaa aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg
    16261 taacaataaa aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt
    16321 gcgacctcta cgatggcggc ggaagaaatt aaagagttgt gtcagagtca taatattcct
    16381 gttgaattaa tccagtgtcg ggttaatgaa atagaaacct atatggatgg tgtgcatttg
    16441 atatgtacca ctgccagggt ggatcgtagt tttggcgata ttccgttagt tcacggcatg
    16501 ccttttgttt ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag
    16561 ggatgaccta tgttttcaga agtcatgcgt tatattctcg acctcggccc tacagtgatg
    16621 ctgccggttg tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt
    16681 aaagcgggtc tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg
    16741 ctggattcca ttggtccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat
    16801 gtggtcgatg tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg
    16861 gtggcgattc cgattgcgat attggttaac gtggcgatgt tactgacccg tatgacgcgg
    16921 gtggtaaatg ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcac
    16981 ctggcaaccg gttcatggat gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt
    17041 tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt
    17101 gctattccgc acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc
    17161 atcgagaaaa tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa
    17221 tttggccctt ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc
    17281 ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg
    17341 ctgctgatgc cacggatgat taaacccatc atggatggtt taacgcccat cgctaagcag
    17401 gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc
    17461 gcattactgc tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc
    17521 attttaattg ctgtttgtgt tccgggtaat caggtactgc cgtttggcga tcttgccact
    17581 atcggcttct tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta
    17641 atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg
    17701 cacacccaac cggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg
    17761 gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt
    17821 cccggtttca ttattatcgg cgcaatttat ctgaccggta ttttcatgac ctggcgtaga
    17881 gcgcgtggtt ttactaaaca agagaaagcc gttctcgcag aataattttt acctgagggg
    17941 gaggttatcc cccttaacaa
//
LOCUS       DBBBSB010000084        17887 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.084, whole genome shotgun sequence.
ACCESSION   DBBBSB010000084 DBBBSB010000000
VERSION     DBBBSB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.084"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(431..3064)
                     /gene="mngB"
                     /locus_tag="ACIUS0_002804"
     CDS             complement(431..3064)
                     /gene="mngB"
                     /locus_tag="ACIUS0_002804"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGB0220488.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(3082..4998)
                     /gene="mngA"
                     /locus_tag="ACIUS0_002805"
     CDS             complement(3082..4998)
                     /gene="mngA"
                     /locus_tag="ACIUS0_002805"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGB0220489.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            5168..5890
                     /gene="mngR"
                     /locus_tag="ACIUS0_002806"
     CDS             5168..5890
                     /gene="mngR"
                     /locus_tag="ACIUS0_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGB0220490.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5993..6862)
                     /gene="sucD"
                     /locus_tag="ACIUS0_002807"
     CDS             complement(5993..6862)
                     /gene="sucD"
                     /locus_tag="ACIUS0_002807"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGB0220491.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6862..8028)
                     /gene="sucC"
                     /locus_tag="ACIUS0_002808"
     CDS             complement(6862..8028)
                     /gene="sucC"
                     /locus_tag="ACIUS0_002808"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGB0220492.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(8122..9339)
                     /gene="odhB"
                     /locus_tag="ACIUS0_002809"
     CDS             complement(8122..9339)
                     /gene="odhB"
                     /locus_tag="ACIUS0_002809"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGB0220493.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(9354..12155)
                     /gene="sucA"
                     /locus_tag="ACIUS0_002810"
     CDS             complement(9354..12155)
                     /gene="sucA"
                     /locus_tag="ACIUS0_002810"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGB0220494.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12456..13172)
                     /gene="sdhB"
                     /locus_tag="ACIUS0_002811"
     CDS             complement(12456..13172)
                     /gene="sdhB"
                     /locus_tag="ACIUS0_002811"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGB0220495.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13188..14954)
                     /gene="sdhA"
                     /locus_tag="ACIUS0_002812"
     CDS             complement(13188..14954)
                     /gene="sdhA"
                     /locus_tag="ACIUS0_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGB0220496.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14954..15301)
                     /gene="sdhD"
                     /locus_tag="ACIUS0_002813"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14954..15301)
                     /gene="sdhD"
                     /locus_tag="ACIUS0_002813"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGB0220497.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15295..15699)
                     /gene="sdhC"
                     /locus_tag="ACIUS0_002814"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15295..15699)
                     /gene="sdhC"
                     /locus_tag="ACIUS0_002814"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGB0220498.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            16080..16187
                     /locus_tag="ACIUS0_002815"
     CDS             16080..16187
                     /locus_tag="ACIUS0_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220499.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16393..17676
                     /gene="gltA"
                     /locus_tag="ACIUS0_002816"
     CDS             16393..17676
                     /gene="gltA"
                     /locus_tag="ACIUS0_002816"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGB0220500.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4273 a         4825 c         4424 g         4365 t
ORIGIN      
        1 ttataagact gaaattagtt tcggttggca attaaagata aaaagagacg aagcaaaaac
       61 agcacggttg accttaattc tttttttggt cactggctaa tacgacgcag agtttcagga
      121 ttattaataa aggcataaat ctccctcccg ccgatattaa catttaatta acttataatt
      181 atattataat taactcaccc gtggttatta ttttgttaaa aataaagcca catatacgta
      241 gcttgagagt gaagataaac acaaaattac ctgatgcgct acgcttgcca gtgtctacat
      301 catctctgca atatattgaa tttgcctgct tttgtagacc gaataaagcg ttcatgccgt
      361 atccggcatg aacaaagcgc cctttgtcaa caatctggcc gcgcatatgt ggcttcggtt
      421 tgattgctat tcaggcaagc cggtaactga acgtccgtga ctggccgggt aaaaaaggcc
      481 ccgatagatt tgaaccttga ttttcctcgg tggtaatgtg ttcatccatc atcgtttctg
      541 agcaagaaat cacctcgcga ctgaaagcaa cagtcgcatc acaggttgct gattcagccg
      601 gattaaacag ccgtaaaatc acttcttgtc ggtcttcagc tttcttaagt gcgcttatca
      661 ggcatcccac tgggggcatt ttcaacaaac tataactttc cggcacgttg aatccggctt
      721 tgttgagctt catcgcatcc catgggattt tgttgtaaca ctgtactgga gtcagccatg
      781 ctcgcgcctg ctgagctaca ccagcggcgg ttggcgtacc ggtataactc aataaactta
      841 ggcgacaaga aagcagacca cgtagttgtg agtccgggac tggcatttta attcccgaag
      901 gccgcctagg ccttaaaagc agatcttctt tccccagtaa gcccacgccg cgcagcaacg
      961 taatggcaaa ggttttcttc tcttcaccga tgacttcaaa ttcacgtagc ccttcgctaa
     1021 agacagccat gccgttacgc ccttcctgta aggctgcata gttgagcata ttccataccg
     1081 gaaccggcgc ttctttccag ctttcttgct gccagttgtt cattgcactg tcgttcacgg
     1141 ggcgcgttag cgaaccaaac tgggtatctg ccagaacact atcggtgtta aaagatgtag
     1201 ggatcaggac gcgaaggcga tgatcgtcag cctggttatc aaggttgata tccacatcaa
     1261 tgcgcctgtt gttatgacta agagtgacaa ccaactcgac atctactctg ccagtggatt
     1321 gtcttgcgct gcgttgttgc agattacgcg gcaccgccat ttcatagcgg ataacagccc
     1381 tgctctgcca ggcctcatga ataatctcgc attgcggttt cgcattcgcc gaagtaatta
     1441 cccactcttc ttttgcgggt gaatagtcat attcatcacc atcatcagag ctttcttcaa
     1501 tttgcaacac ccggtcatag cgcacaccgc tatctttatc taccagttgt agcgttccat
     1561 cctcattgag cgcaatttgc cagaaagcat tttccagtat cccttcagcg tcactttttg
     1621 ccgcaatcac gttgccaggc tgattcgctt cgatataaag cgtgcgatag cccatagaag
     1681 ggacaatctg gttgatctgt atatcaaact ccataaaggg atcgtaatta ccgtaatgaa
     1741 ctatttgccg atcgattagg cctggatcga tctcacgggc atggcgaata aaatacggta
     1801 caggctgacc gcgatcgtct cgtagattaa actggctggc gcgcagccgc acagtggtgt
     1861 tgatcacttc ttcccgcggc cagggcatca ggttaaacag gacgagtttg tcggcgtcgc
     1921 tctgcggcat gttgtcggcg attttacgca tatagaaacg cagcagatta tccgccatgt
     1981 cttcagccag ttcgaagcgg gcgacgattt cgcgatgaac tttgtcacga cagcagcagc
     2041 cgatgctgtc gtgggcatga ttttttaaga tctctttcca cattttctcc agcaagccgt
     2101 ggtggtatcc aaaacccaac gtcctggcca gcgttgccag cggttccagc agattaacaa
     2161 tcttattttc aatacgtgcg tgggcaactt tgatatccat gcgcgtggaa ccgatggtgc
     2221 gatgcacgcg catatatttg ccatcaataa attccccttt cagggttgcc agattatctc
     2281 gctgcgcttc gatcttctca aatacctctt caaagcggct catcacaaac tttcgttgag
     2341 gataaatttc gcgcagtttc tccatcactt cgaagatatt ttgctgcaat ggcatctgat
     2401 catgcccatt cggcagcaaa atctctttgg ttacagacgc tttttccagc acgtcaaaat
     2461 aactgtcgag gcgtttgcgt aatccgtttt cgtccgcagg taagtactta ccgatggcat
     2521 aacccaacgg catcacctgc gccgtcactt cgctaccgtc actgctttgc cacaaaaact
     2581 cggttttatc cgtaccgtgg cgctcagaac atccacgcca gaacatggtg caggtaatgc
     2641 caaatccatt gtagatatgc ggcagttgcc cggacatgcc aaaggaatcg ggtaaataac
     2701 ctattttcat cggctcgcca aacgcgaggc agtcacgcat tccgtacatc agattacgga
     2761 cgatggattc cgcagaaaca atcgtggtat cggtctgggt ataccagggg ccgataatca
     2821 acttgccggc ttctaccagt ttcttcaagc ggtctttgtt ttccggtttc accgcgaaat
     2881 aatcttcgag gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg ttgtcctgtt
     2941 ccagtcggca caggatctct tccatattat tgaccagcag aatgcgtgac tcttcggtgg
     3001 tgaaatacca ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga gataccgctt
     3061 tcatcgtcgt ttcctttttg tttatggcat tacgccatca gtcagatagt tgccatgctt
     3121 aaccgcgtga cgccgccaga tcaggagaat tgcagtcgag attgcagccc ccaccaatgc
     3181 cgcaccaaac cagccaattg ccgccataac accgcccgcg ccattatcat gaagtaaaaa
     3241 gagcgagaaa atgccagcac cgggtgtcga aaggccgata ttcatcgcac cgacaatagc
     3301 gcccgttacc atagagccca gcacaaacga accaataacc cgcagcggat cttcaatcgc
     3361 catcgggatc gccccttcgg taatacctgc cagccctaac agccaggtgg atttcccggt
     3421 ctcaatttca aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg ttacggtaaa
     3481 tgccgaaacc attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc cgttcgccat
     3541 tgcgcccagg cagaatgcat aagcggcttt attcactggc cctccaaggt caaaggaaca
     3601 cataaaaccg agaatggcac ccagcaacag cgcgttactt cctgacagac cgttcagcca
     3661 ggcggtaagc gagttattga tccaggcgac aggttccccc accacaaaca gcatcagact
     3721 gcccgctccc aatgtaccga gcaccgggta gagataaaaa gtcaggaagc cattgaattt
     3781 actgctaaga cgcaagtgat ttttcatcca gcgtatcaag taaccggcta tcaatccgcc
     3841 aacgaccgcg ccgagaaacc cggagccgat catgttggcg gcaagtccag ccgcaaagcc
     3901 tggcgctaac gccggtttat ctgccagcga ataggcggta taggctgcca gcaccggtac
     3961 catcaaaatt ccgagcagcc cgccgcccag tttgcggtac atccacagcc aggagttttc
     4021 ttcattaaac agatcttgta gcccgaagat ttgcgacagt aataccgcga ccgccagcac
     4081 cgtccccccc gcgacaatca acggtacggc aaaagagatc ccgctcaaca gtgcctgttt
     4141 cagctccgtt ttgacgcttt tcaccggttg cgtatcttgc tgtaaggtac gcgtctcatc
     4201 gccacgctcg agggtaagcg attgttggat caacgcttct gcatggcgaa tcggctcggc
     4261 aacaggcaca gaaagcgcag gaatgccgtt aaaacgctca ctctccttga tggcgacttc
     4321 agccgcaaaa atacaggcag ttgcactatt gagttgatcc gccgttaaac gcccttcaat
     4381 accgttagca ccttgctttt caacgtagac attcacgccg agtttgcgtc cggctttttc
     4441 cagatactcc gcagccatgt aggtgtgagc aatacccgcc gggcaggccg ttacgcaaac
     4501 gatagttggc gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt catccagcgc
     4561 cgagagcaat tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct catcatccgc
     4621 caggcgcgtg gtcagcgctg tcagcagttg catgtgcgta gtacccgctt cattgagggg
     4681 aatcgccagc aacaccacta aatcaaccgc ttccgggcca tccacgcctt cccactgaag
     4741 cggctcgctg agtgtggcga ccgcaaacgc cgcttctttt accgcagcag ttttgccatg
     4801 cggcacagcc aacccttcac ttaaggcggt cgggccaagg ctttcacggc gatacacttc
     4861 tttcagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt gagtcaacgc
     4921 gtgaatggcc tcttcacggc tggtaaagcg cgcattcaga cacaacgcat cgcggtgggt
     4981 cagagtcgtc aggttcatca tgatcctcac ttgatcgttt ttatagtcgc gccagtgtgc
     5041 ccgacaaaac aataccattg cgtaatcaat ttcacttgat tgaaattatt tgcattttat
     5101 ttgtattgtt tacaggatga gtttatcggg cataatggct aaattcgtat taatgagatg
     5161 ctaaataatg ggacacaaac ccttataccg acagattgct gatcgcattc gtgagcaaat
     5221 tgcgcgtggc gagcttaaac ctggcgatgc gttacctacc gaatcggcat tacaaacaga
     5281 gtttggcgtc agccgggtta cggtgcgtca ggctttgcgc cagttagtcg agcaacagat
     5341 cctcgaaagc attcagggca gcgggactta cgtcaaagaa gagcgggtca attacgatat
     5401 ttttcagtta accagttttg atgaaaaact gtcggaccgt cacgtcgata cacacagtga
     5461 agttctgata tttgaagtga ttccggctga cgattttctt cagcaacagc tacaaatcac
     5521 cgtgcaggat cgtgtttggc atgtgaagcg cgtgcgctat cgcaagcaaa agccaatggc
     5581 gctggaagaa acctggatgc cgcttgcgtt gttcccggat ctcacctggc aggtcatgga
     5641 aaattcgaaa tatcacttta ttgaagaagt gaagaagatg gttatcgatc gtagcgaaca
     5701 ggaaatcatt cctctgatgc caaccgaaga gatgagccgt ctgcttaata tcagccagac
     5761 aaagccgatt cttgaaaaag tatcgcgtgg atatttagtt gatggtcggg tgtttgaata
     5821 tagccgcaac gcttttaata ccgatgacta taaatttacc ctgatagcgc agcgaaaatc
     5881 atcgcgataa gcacaaaaag gccatcatac gatggccaac catgtcgaaa ccgacagccc
     5941 ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat atttatttca
     6001 gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc acgcctgcgg
     6061 cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg atcgcacccg
     6121 cgtggcccat acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc acaactggct
     6181 tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg ccgatctcac
     6241 cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga atgtcgataa
     6301 agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga ccgaaaccgt
     6361 aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa acgataccca
     6421 ctttacccgg tttgtgaatg tgacccggct ggataccaat tttgcattca cccggggtga
     6481 taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt ttcactttca
     6541 cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt ttgatgcctg
     6601 catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa ataacagacg
     6661 cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc aggccgaggt
     6721 gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg tatgcaatgg
     6781 cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata accttggtgt
     6841 ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct gctgagctgc
     6901 atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt tcttcgcgcc
     6961 gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac ccacttccgc
     7021 taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc cgaagatgtt
     7081 aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt cggttacacg
     7141 ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc cgtgcagttt
     7201 aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac cgatgttacc
     7261 gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg gatcttcctg
     7321 cgactggtca cgcatttcgc gcagatcagg ctggcggaac agggcgttgc catcagcgcc
     7381 cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg ggttgatttc
     7441 gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga ttttggtgaa
     7501 ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc cctgatacgg
     7561 catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag tttcttccgc
     7621 cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc gacgggaact
     7681 acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg cttcaaccag
     7741 aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac gcttgcccag
     7801 ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca caccgcccgc
     7861 tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg caccgatttt
     7921 tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg gtgctggtaa
     7981 gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt gttctgtcca
     8041 tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg tcggtctaca
     8101 gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggct cttccagtaa
     8161 ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac ggtgatcgta
     8221 ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg ccatcggacg
     8281 atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga tcggcgtaga
     8341 catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg tcagatcttc
     8401 aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct tctcgatgtc
     8461 tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc gcggcgtaga
     8521 aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt cgatagacgc
     8581 gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga aggacataaa
     8641 gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac gcagatccat
     8701 aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt ttttcgcttc
     8761 cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt tttcactacg
     8821 tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact ctttcgccgg
     8881 ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca caccggtgcc
     8941 tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg ccgggcttaa
     9001 cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag tggacgcttt
     9061 ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca ggcgaccaag
     9121 gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca gaatgccgtc
     9181 tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca gcacttcatc
     9241 acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat cggctacgga
     9301 ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt tatttattcg
     9361 acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga catataccct
     9421 accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc aaacggaatc
     9481 acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg ctcttcctgg
     9541 caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat cgctttatgc
     9601 gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt gttcttacga
     9661 cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg cttcacgccc
     9721 ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt cgccagttct
     9781 tcgaggctgg aaaccgccag cggatgacgc agcagggatt tcggcgacat cacgaccagc
     9841 ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg tgccggggta
     9901 gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg ttccagacgc
     9961 gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat caccagacca
    10021 cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac cacctgcgca
    10081 ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg ttctgcggtg
    10141 gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc ccagacacgg
    10201 aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc gttagactgg
    10261 ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc acccgacagg
    10321 cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc accgccccag
    10381 tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt ggcaacgcga
    10441 gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc ctgcaggcgc
    10501 ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta cggcgaccag
    10561 gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc agcatccagc
    10621 gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcgccac tttttcctgc
    10681 tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt ctgatacatc
    10741 agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg gtagcatacc
    10801 aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag acgggtcaca
    10861 aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg aaccatctta
    10921 ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt ggtgaaacca
    10981 acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg cgctttcgac
    11041 atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc gtggatggtg
    11101 attggcagca ctttgttgct gttcggctca tcaagtctgt ccagacgggc acgaacagag
    11161 ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag cgccaggtgc
    11221 accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt cacgtcgcct
    11281 gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg cggtttttta
    11341 cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag aaccacttcg
    11401 cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat taacgcgtca
    11461 ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta acgctcaagg
    11521 ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt gaaagtcgcg
    11581 cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt gatgtgcata
    11641 tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag ctcgccgagt
    11701 ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg gaagtctgct
    11761 tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg ccacagtccc
    11821 agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat gagctgcagg
    11881 actttaacct gcttcacatt ggtgtcaggg tcggagatcg ttgaagagta acgtgaagcg
    11941 tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc cggtttgact
    12001 cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga gtcaggatcg
    12061 gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga gaggtaagaa
    12121 gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc atctttttta
    12181 tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg ttcacttgcg
    12241 agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta gtgtcagggg
    12301 cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc ttatcaggcc
    12361 tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca ctggttgcct
    12421 gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg caacaacatc
    12481 gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac actgacgcag
    12541 ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc gaggcggctg
    12601 tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa gcctgccggg
    12661 ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca acatgcgcag
    12721 agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg taaatgctcg
    12781 cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc atattgcgca
    12841 tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg cagcgggcga
    12901 atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca ggccagacca
    12961 ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca ggagcggcgg
    13021 aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag catcatgtcg
    13081 cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc aacatccggg
    13141 ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta gtaagtacga
    13201 atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg gcgcgtcatg
    13261 gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc acgatccggg
    13321 aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga aacagccgtt
    13381 gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg ggtgttgaac
    13441 tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac tttcaactgc
    13501 tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg ctgcatacat
    13561 tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt gttccaccgg
    13621 ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgtgcag tgcgccctgc
    13681 tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac caggtccagc
    13741 agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat ttcaccaacg
    13801 gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag cgcctgaccg
    13861 gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac cggaatcggc
    13921 tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc cggcagacgg
    13981 gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg cccccacgga
    14041 ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac cacatcacgg
    14101 cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc atgtttgttc
    14161 agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc accggcaatg
    14221 ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc gcggatagcc
    14281 atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata aatacgccct
    14341 gctccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc accggtttcg
    14401 atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag atccagcgca
    14461 taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag cgtgtgcaac
    14521 agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc gccgccgaag
    14581 tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag acgcgagaac
    14641 ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca catatattca
    14701 atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat atgccattcc
    14761 cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc agaaacggta
    14821 tgggaacggg tcgggaagac tttagagagc agggcacagg tctggccgct ctgggaaatt
    14881 tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc aaattctctg
    14941 actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg taaaccacca
    15001 gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta acgtagtcgg
    15061 tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc gccagcaggg
    15121 tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat gtcagttcgc
    15181 cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg atagcggtag
    15241 cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg ttgcttacca
    15301 tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga ttttggcgga
    15361 gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca tgtggcgaat
    15421 acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa atttgacgaa
    15481 gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga ggctggtacc
    15541 cagaagccac tgcaggatgc ccactgcaac aaaggtgatc acaccggaaa cgcgatggag
    15601 aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat taacaggtct
    15661 ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat tattccctgg
    15721 ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt tccttcctcc
    15781 ggtctgcttg cgggtcagac agcgtccttt ccataactgc gcgtcatgca aaacactgct
    15841 tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag ggtgttccgg
    15901 agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac atatagtttg
    15961 tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt aatttttaat
    16021 catctaattt gacaatcatt caacaaagtt gttacaaaca ttaccaggaa aagcatataa
    16081 tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac agtgtggaag
    16141 tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa ttcggattgc
    16201 taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca agcgcccaga
    16261 gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac aaacgggtaa
    16321 agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa taaggcgcta
    16381 aggagacctt aaatggctga tacaaaagca aaactcaacc tcaacgggga cacagctgtt
    16441 gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg tactctcggt
    16501 tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg cgaatctaaa
    16561 attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc gatcgatcag
    16621 ctggcgaccg attctaacta cctagaagtt tgttacatcc tgctgaatgg tgaaaaaccg
    16681 actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat gatccacgag
    16741 cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc agtcatgtgt
    16801 ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa caatcctcgt
    16861 catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc cgcgatgtgt
    16921 tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc ctatgccggt
    16981 aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa tccgattctg
    17041 gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa cgcctctacc
    17101 tccaccgtgc gtaccgctgg ctcttcgggg gcgaacccgt ttgcctgtat cgcagcaggt
    17161 attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct gaaaatgctg
    17221 gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa agataaaaat
    17281 gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga cccgcgcgcc
    17341 accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa agatgacctg
    17401 ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt tatcgagaag
    17461 aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat gggtattccg
    17521 tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat cgcccactgg
    17581 agcgaaatgc acagttacgg tatgaagatt gcccgtccgc gtcagctgta tacaggatat
    17641 gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa gttgtaaata
    17701 ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca aaaaattgta
    17761 aaaataaaat ccactatcgg acattcatat attctaaaaa aaaatacatc ggtataaatt
    17821 ataaacaaat agatgatttt taatcatcta tcttcctctc agttacatga ataaaccacc
    17881 aaatata
//
LOCUS       DBBBSB010000085        17881 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.085, whole genome shotgun sequence.
ACCESSION   DBBBSB010000085 DBBBSB010000000
VERSION     DBBBSB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17881
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.085"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            277..960
                     /locus_tag="ACIUS0_002817"
     CDS             277..960
                     /locus_tag="ACIUS0_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGB0220501.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1004..5666
                     /locus_tag="ACIUS0_002818"
                     /pseudo
     CDS             1004..5666
                     /locus_tag="ACIUS0_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6034..7689
                     /gene="lldP"
                     /locus_tag="ACIUS0_002819"
     CDS             6034..7689
                     /gene="lldP"
                     /locus_tag="ACIUS0_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGB0220502.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7689..8465
                     /gene="lldR"
                     /locus_tag="ACIUS0_002820"
     CDS             7689..8465
                     /gene="lldR"
                     /locus_tag="ACIUS0_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGB0220503.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8462..9652
                     /gene="lldD"
                     /locus_tag="ACIUS0_002821"
     CDS             8462..9652
                     /gene="lldD"
                     /locus_tag="ACIUS0_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGB0220504.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9848..10321
                     /gene="trmL"
                     /locus_tag="ACIUS0_002822"
     CDS             9848..10321
                     /gene="trmL"
                     /locus_tag="ACIUS0_002822"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGB0220505.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10374..11195)
                     /gene="cysE"
                     /locus_tag="ACIUS0_002823"
     CDS             complement(10374..11195)
                     /gene="cysE"
                     /locus_tag="ACIUS0_002823"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGB0220506.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11275..12294)
                     /gene="gpsA"
                     /locus_tag="ACIUS0_002824"
     CDS             complement(11275..12294)
                     /gene="gpsA"
                     /locus_tag="ACIUS0_002824"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGB0220507.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12294..12761)
                     /gene="secB"
                     /locus_tag="ACIUS0_002825"
     CDS             complement(12294..12761)
                     /gene="secB"
                     /locus_tag="ACIUS0_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGB0220508.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12824..13075)
                     /gene="grxC"
                     /locus_tag="ACIUS0_002826"
     CDS             complement(12824..13075)
                     /gene="grxC"
                     /locus_tag="ACIUS0_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGB0220509.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13217..13648)
                     /locus_tag="ACIUS0_002827"
     CDS             complement(13217..13648)
                     /locus_tag="ACIUS0_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGB0220510.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13893..15437
                     /gene="gpmM"
                     /locus_tag="ACIUS0_002828"
                     /gene_synonym="pgmI"
     CDS             13893..15437
                     /gene="gpmM"
                     /locus_tag="ACIUS0_002828"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGB0220511.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15471..16730
                     /gene="envC"
                     /locus_tag="ACIUS0_002829"
     CDS             15471..16730
                     /gene="envC"
                     /locus_tag="ACIUS0_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGB0220512.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16734..17693
                     /locus_tag="ACIUS0_002830"
     CDS             16734..17693
                     /locus_tag="ACIUS0_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGB0220513.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17680..17881)
                     /locus_tag="ACIUS0_002831"
                     /pseudo
     CDS             complement(17680..17881)
                     /locus_tag="ACIUS0_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4490 a         4636 c         4656 g         4099 t
ORIGIN      
        1 tcacaaaatc aattatctgt gaaatttttc ccagacatgg tttctttcat atttgacatt
       61 tattaataat catcattaga atccacgaaa aataatatat tcatacaaac acagacatct
      121 tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca
      181 tcactgtcat taatgtaaag caacatagct caccaataat acaatggaca actctaccgg
      241 cattgagcgc tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc
      301 agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc
      361 aagccagaaa gtagtttact gaaacaacaa gaagaacata ataataaaat tgtgctactt
      421 gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc
      481 ataataaaca gtacaaagac tttaactcac gtaattaaat ccgttaaaga ccaacaaaat
      541 acttttattt ttaccgagtt taacccagca aaaacgaagt atttcatcct gaacaacggt
      601 tcggtggctt tagcgggtcg agtgttatcc attgacgcca cagagaatgg tagtgttatt
      661 catatctcac tggtcaactt attaagtaca cctatctcaa acattggttt taatgcgaca
      721 tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca attacttttc
      781 aacacatcaa tgaaatccac attgaaatta ttaccaggtc aatggcaaga cattaatttg
      841 accctgaagg gtgtatcgcc caataacctg ggatatctga agttagccat caacatggaa
      901 aatattcagt tcgacaatct tccctctgct gaaaatcggc agaaaagaag caaaaaataa
      961 atgcagttag tacgcttata acaaggaata gcgagcatca ttaatgaaca aaatatttaa
     1021 agttatctgg aaccctgcga cagggaatta tactgttacc agcgaaacgg caaaaagccg
     1081 tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt
     1141 gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg
     1201 tagtggatca gctggcgacg gctgggttgc tataggcaaa ggggcgaaag caaatacttt
     1261 tatgaacacc agtggttcca gtactgctgt gggttatgac gctatagctg aaggccaata
     1321 tagctctgcc atcgggtcaa aaacccatgc gattggtggt gcatcaatgg cctttggggt
     1381 tagtgcaata tcagaaggcg atagaagtat agcactgggt gcctcttcgt attcattggg
     1441 ccaatactca atggccctcg gccgttattc aaaagcattg ggtaaattgt ctattgctat
     1501 gggggactct tccaaagcgg aaggagcaaa cgccattgcc ctggggcgta gcagtgtagc
     1561 tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag cgaacgctat
     1621 tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca ataatgcgaa
     1681 ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata aagtcaatac
     1741 tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca taggccaggg
     1801 caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt cgagaggctt
     1861 aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc ttgcgcttgg
     1921 tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt ccattgcgga
     1981 tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg ttaatgtgaa
     2041 aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc tttatgccat
     2101 tagcgactcg gtagcaaaaa aggcttggag gaggggctgc agtagatgtt gatgacggta
     2161 ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc
     2221 tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg
     2281 ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt
     2341 cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg
     2401 aagccaacac caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg
     2461 ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca
     2521 gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg
     2581 gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata
     2641 acacttccaa tattgccact aacaccacca acatctctaa cctgactgag acggtgacta
     2701 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg
     2761 gcaccgagac caccagcaaa ataaccaacg ttaaagatgg cgacctgacg actggcagca
     2821 ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca
     2881 ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2941 gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca
     3001 ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg
     3061 ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca
     3121 atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa
     3181 attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg
     3241 gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg
     3301 gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg
     3361 cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg
     3421 gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg
     3481 attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcctgac gacgctctgc
     3541 tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa
     3601 ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga
     3661 ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg
     3721 cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt
     3781 acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg tgggataaga
     3841 ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca
     3901 ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt
     3961 tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta
     4021 acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt cgtactaacg
     4081 acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca gctgtaggtt
     4141 ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc agcagcaccg
     4201 ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt gccaaaggtt
     4261 ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc actgacggcg
     4321 aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc atcaacgtag
     4381 ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac gctattggag
     4441 cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa gactcactgg
     4501 ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt aatgcaggta
     4561 ttggtatcgg tttgaacact ctggttctgg ctgatgcgat caacggcatt gctatcggca
     4621 gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct cagaccaccc
     4681 gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac tctgtaggtg
     4741 agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca gctggttcag
     4801 cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc gtagcgcaaa
     4861 atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta
     4921 atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac ttcaagacca
     4981 acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt
     5041 ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa
     5101 atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccagtg
     5161 ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggagtgc
     5221 gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca
     5281 acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga
     5341 actacgcgca gttgaagcaa agcgcgcagg aaacgaagca atacaccgat cagcggatgg
     5401 ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg
     5461 caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg
     5521 gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca
     5581 atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg
     5641 cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg
     5701 ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg tcaacaatct
     5761 aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc cccacaagaa
     5821 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5881 acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta tccctacaca
     5941 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
     6001 tacacgatgt gcgtggactc taggagacct gcaatgaatc tctggcaaca aaactacgat
     6061 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
     6121 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtagcaatc
     6181 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     6241 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
     6301 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6361 ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6421 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggcctg
     6481 ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca
     6541 tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat cgacagcttt
     6601 gagattggcc agatggtggg gcgtcagcta ccgtttatga ccattatcgt gctgttctgg
     6661 atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc ggtcgtggtt
     6721 gcaggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
     6781 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
     6841 caaccagttc gcgtattccg cttcggtgac ttgggggcgt cacaggttga tatgacgctg
     6901 gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg
     6961 accgctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt
     7021 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcaataaact ggttgcccgt
     7081 atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
     7141 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
     7201 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctacccatc
     7261 tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg actgtcatca
     7321 acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7381 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
     7441 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
     7501 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
     7561 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7621 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
     7681 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7741 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg
     7801 cgatgcaact cggcgtatca cgtaattcac tacgcgaggc gctggcaaaa ctggtgagcg
     7861 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
     7921 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
     7981 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
     8041 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
     8101 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     8161 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
     8221 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
     8281 aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa
     8341 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8401 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
     8461 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8521 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
     8581 agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg acttaagcct
     8641 ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac cggtgggttt
     8701 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
     8761 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
     8821 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
     8881 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
     8941 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg
     9001 ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     9061 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
     9121 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     9181 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
     9241 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9301 ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga
     9361 tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
     9421 gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg
     9481 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9541 gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc aggggctggg
     9601 taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc
     9661 gccccctcac cctaaccctc tccctcaggg agaggggacc gttcggcgct gtatgtactc
     9721 cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg ggagggccgg
     9781 ggtgagggga aaaggccgca cggttcacca attttctgct atcctgcccc cgcactaagg
     9841 gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata ctggcaacat
     9901 catccgtctt tgcgctaata ccggctttcg tctacatatc atcgaaccga tgggatttgc
     9961 ctgggacgac aagcgcctgc gccgcgcggg gctggactat cacgagttta ccgccgttac
    10021 gcgtcatcat gactatcgcg cgttcctcga agcagaaaat ccccagcgcc tgtttgccct
    10081 caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg attatctgat
    10141 gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc ccgctgaaca
    10201 aaaaattcgc attccgatgg tgccggacag ccgcagcatg aatctgtcca atgcggtgtc
    10261 ggtagtggtg tatgaagcct ggcggcagct gggatatccg ggagcgttgt tgagagatta
    10321 gttactgtat gccggataga tgattacatc gcatccggca cgatcacagg acattagatc
    10381 ccatccccat actcaaatgt atggttaata ccgttgaaat gctggtccat atccattgat
    10441 ggcttatcgc tgtctggttt accgacaata cgagccggaa cgccagcggc ggttgtatgc
    10501 ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg accaacttca
    10561 atattgccga ggattttcgc gcccgcgcca atcatcacac cttcacgaat tttcgggtga
    10621 cggtcaccac cagatttacc cgtaccgcca agcgtcacag attgcagaat cgatacgtcg
    10681 ttttcaatta ccgccgtttc acccacaacg atgcccgtgg cgtggtcgag cataatgcca
    10741 cggccaattt ttgctgccgg gtgaatatcg acctggaacg tcacagaaac ctggttttgc
    10801 agaaagattg ccaatgcgcg acgcccctga ttccacaacc agtgaccgat gcgataggcc
    10861 tgcaaggcat gaaaaccctt caggtataac aacggggttg agtatttatc gactgccggg
    10921 tcgcgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc cgggtcagcg
    10981 gcgtaggctt cttcaaccac ttcacggata gcaatagcag gcataattgg cgatgacagc
    11041 ttgttcgcca gcatgtagct cagtgcactg ccaaggtttt cgtgcttgag tagcgtcgcg
    11101 tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc ggctttaata
    11161 ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg atgagataat
    11221 gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca aaggttccct ggggttagtg
    11281 actgctgcgc tcgtccttgc gtgcacgacc taataatgtc aatgctgcct cgcgcgcgtt
    11341 ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcaa cgccgaagcg
    11401 atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct gaccaatctt
    11461 ctcctgcgcg ctttgtacat ccatgccctg accgagcatc ataccaaaac ggcggttacg
    11521 cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc ccataaaggt
    11581 ggcaggatcg gcacccagcg ccgcaccaag acgcgacatt tcagccagcc cacgggtgat
    11641 cagcgccgta cgcgcattcg caccaaaacc gataccgtca gacatccccg cgccaatggc
    11701 aatgacgttt ttcaccgcgc cgccaagctg cacgccaatg aaatccgggt tgctgtaaac
    11761 gcggaaactt ttgccgcagt gcagcagctg ctggagatca tcggcaaagg tctgatcggt
    11821 cgaggccagc gaaatagctg tcggtaaacc tgccgccagt tctttcgcaa acgttgggcc
    11881 agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt cctgtaacag
    11941 acgtccggtt tccgcttcca gccctttggt cgcccacacc agacgcgcat caggacgcat
    12001 cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga cgacgagaat
    12061 attacgactg gctgccagcg cagtggcgag atcgctttca agatggagcg tatcgggaaa
    12121 aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg cgatatgttc
    12181 agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtaa tggcaagagc
    12241 ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt tcatcaggca
    12301 tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat gaacagcgca
    12361 tcgaagttaa ccggcgcaag gttcagttgc gggaatgtac cgcgggatac catgctggtg
    12421 atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag gcaatgtgcc
    12481 atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac ttcacacagg
    12541 aacgcggttt cttcgcccaa ggaggccgtt acggtaacac gcagtaccac ttcgtatacg
    12601 tcatctgcca gttgggaaga tgccgtatcc agatcaagtt taacttctgg ttgccaatct
    12661 ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga tatccttggt ataaatacgt
    12721 tggatctgga aagtcatttc agtgttgttt tgttctgaca tgtgtagaaa acccttaagt
    12781 gttgtcctta aatactgcgc aatgtaatac caacgttcac acgttatttc agcaggggat
    12841 ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg tgctgtgcgt
    12901 caataaaaat ctggggaacc gtggtgcaac cgctgcgttt gatcatctct tcacgcttgg
    12961 cggcattgcc atcgatcggc aactcctgga aactcacgcc cttgctgctc agcagtgctt
    13021 ttgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg gccataattt
    13081 agctcctgtt tttttaccct gaaatttcat gttgcagggc ggctgcaaaa tggagtgtcc
    13141 ccagaagctt ataacaataa gtcgctgggg gcctaattgc agctaacggc ctgcatcatg
    13201 aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa gttttcgcca gcccagccag
    13261 caacgccctc tttcagcacg aatacttgcg caaaaccagc tttcgtcagc gcgtttgcag
    13321 gctcctggca ctgcatgcca gaaccgtcta ccacgataac cgatttgtct ttgtgcttct
    13381 caagctcacc aacattgttg gctttgattt cgctcggcaa cagattgata gaacctgcga
    13441 tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg tcttctttgt
    13501 tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa
    13561 acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt atgggatgac
    13621 ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa
    13681 aaaaccagga aaggagtata cctgcgcggt gcggcaaata cagccagcgc gttaactgga
    13741 atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc tggcatcggc ttgccgcgag
    13801 cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc
    13861 aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac
    13921 tggtgattct ggatggctat ggctatcgtg aagaacagca ggataacgcc atttttagtg
    13981 ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt
    14041 ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta
    14101 acctgggtgc cggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaag
    14161 atcgcgcttt ctttgctaat ccagtgctgg ctggcgcggt acataaagcg aaaaacgcag
    14221 gtaaagcagt acacattatg ggtctgctct ccgcaggcgg cgtacacagc cacgaagatc
    14281 acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg
    14341 cattccttga cggtcgcgac accccgccgc gcagtgctga atcctcgctg aaaaaatttg
    14401 aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc catcattggt cgttactacg
    14461 cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ctatgacctg ctgactctgg
    14521 cgcagggtga gttccaggcc gatactgccg ttgctggttt gcaggcagct tatgctcgcg
    14581 acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg
    14641 caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc gcgcgtgaaa
    14701 tcactcgtgc attcgtgaac gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg
    14761 tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa aactgcggtt gcttacccac
    14821 ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc
    14881 gtatttccga aaccgaaaaa tatgcccacg ttactttctt cttcaacggt ggcgtagaag
    14941 agtcgttcaa aggcgaagat cgcattctga tcaactcacc gaaagtggct acctacgatc
    15001 tgcaaccgga aatgagctcc gcagagctga ccgaaaaact ggttgcggcc atcaagagcg
    15061 gcaaatacga caccattatc tgtaactatc cgaacggcga catggtaggt cacaccgggg
    15121 tgatggaagc ggcggttaaa gcggttgaag cactggatca ctgcgtggaa gaagtcgcga
    15181 aagcggttga gtccgtgggt ggacaactgc tgatcaccgc tgaccacggt aacgctgaac
    15241 agatgcgcga tccggcaacg ggtcaggcac acacggcaca caccaacctg ccagttccgc
    15301 tgatttacgt tggtgataag aacgtgaaag cggttgcagg cggcaaactt tctgacatcg
    15361 cgccgaccat gttgtcgctg atgggtatgg aaatcccgca agagatgact ggtaagccgc
    15421 tgttcatcgt ggaataatcc ctccccatga ggggaaaggc gattaatacc atgacacggg
    15481 ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt cttagcgctg
    15541 gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg tgaccaactc aaatctattc
    15601 aggccgatat cgccgcaaaa gagcgcgcgg tacgccaaaa gcaacaacaa cgcgcaagcc
    15661 tgctcgcaca attgaaaaag caggaagaag cgatctctga agccacccgt aagctgcgcg
    15721 aaacgcaaaa cacgctcaat caactcaata aacagattga tgagatgaac gcgtcgattg
    15781 ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct cgccgcacaa ctggatgccg
    15841 cattccgtca gggcgagcat accggtattc agctaattct cagcggtgaa gaaagccagc
    15901 gtggacagcg tttacaggct tatttcggct atctcaacca ggcgcgacaa gaaaccattg
    15961 cccagttgaa gcaaacgcgt gaagaagtca ccatgcagca tgctgaactg gaagagaaac
    16021 aaagcgagca acaaacgctg ttatatgaac agcgcgccca acaggcgaaa ctgactcagg
    16081 ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc ttccatccag caaggtcagc
    16141 agcagctaag tgaactgcgt gccaacgaat cccgtctgcg taacagcatt gcccgtgctg
    16201 aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga ggcccaggcg gttcgcgagc
    16261 gccagaaaga agcgacgcgc aaaggcacca cctacaaacc gaccgaaagc gaaaaatcgc
    16321 tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca ggcattctgg ccggttcgcg
    16381 ggccgacgct gcatcgctat ggcgagcagc tacagggtga attacgctgg aaagggatgg
    16441 ttatcggtgc ctctgaaggt actgaagtta aagcaattgc cgacggtcga gtgattctgg
    16501 ctgactggct gcaaggttac ggtctggtgg tggtggttga gcacggtaaa ggcgacatga
    16561 gtctttacgg ctataatcag agcgcactgg tgagcgttgg ttcgcaggtt cgcgcgggcc
    16621 agccaattgc actggtgggc agcagtggcg gtcagggtcg gccttcactc tatttcgaaa
    16681 ttcgccgcca gggtcaggcg gtcaatccac agccgtggtt gggaagataa gttttgtttc
    16741 catttcgtcg taacgttctt gcatttgccg ctctgttggc gctctcctcc cccgtacttg
    16801 ctggcaaact tgccatcgtc attgatgatt ttgggtatcg cccgcacaac gaaaaccagg
    16861 tgctggcgat gccttccgct atctccgtcg ctgtattacc cgattcaccg cacgccagag
    16921 agatggcgac caaagcgcat aacagcggtc acgaagtgtt gattcatctc ccgatggcac
    16981 cgttgagtaa acagccgctg gagaaaaata cgctacgccc ggagatgagc agcgacgaaa
    17041 ttgagcgcat tattcgtagt gcggtcaata acgtgcccta tgccgtgggg atcaacaacc
    17101 acatgggcag caagatgacc tctaacctgt ttggtatgca gaaagtgatg caggcgctgg
    17161 agcgttacaa tctttacttc ctcgacagcg taaccatcgg taatacccag gcgatgcgcg
    17221 ccgcgcaagg tactggcgtg aaggtgatca aacggaaggt gttcctcgac gattcgcaga
    17281 acgaagcgga catccgtgtg caatttaatc gcgcaattga tctggcgcgt cgcaacggtt
    17341 caaccattgc cattgggcat cctcaccctt caacggtacg cgtgctacaa cagatggttt
    17401 ataacctgcc gccagacatt acgctggtga aagccagcag cttgctgaat gaaccgcagg
    17461 ttgatacttc tacaccgccg aaaaacgctg tgcctgatgc accgcgtaat ccgttccgtg
    17521 gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt ttatgctaat cgcttctttg
    17581 aagtattaag cgaaagcatc agccagagca cgctgatcgt ttacttccag catcagtggc
    17641 aaggctgggg caaacagccc gaagcggcga agtttaacgc tagcgcaaat taaaggcgct
    17701 acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat tgccataagc ggaaagacca
    17761 cagtaacgcc tggtaaccga ccttcacgct gcgcacattg gtaatcaggc gcttatacat
    17821 acccggaagt aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc
    17881 g
//
LOCUS       DBBBSB010000086        17644 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.086, whole genome shotgun sequence.
ACCESSION   DBBBSB010000086 DBBBSB010000000
VERSION     DBBBSB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.086"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACIUS0_002832"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACIUS0_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACIUS0_002833"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACIUS0_002833"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGB0220514.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACIUS0_002834"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="ACIUS0_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACIUS0_002835"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="ACIUS0_002835"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGB0220515.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYILPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACIUS0_002836"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="ACIUS0_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGB0220516.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="ACIUS0_002837"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="ACIUS0_002837"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGB0220517.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="ACIUS0_002838"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="ACIUS0_002838"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGB0220518.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACIUS0_002839"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="ACIUS0_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGB0220519.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTVDQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACIUS0_002840"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="ACIUS0_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGB0220520.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACIUS0_002841"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="ACIUS0_002841"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGB0220521.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8138..9471)
                     /gene="astB"
                     /locus_tag="ACIUS0_002842"
                     /pseudo
     CDS             complement(8138..9471)
                     /gene="astB"
                     /locus_tag="ACIUS0_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9468..10946)
                     /gene="astD"
                     /locus_tag="ACIUS0_002843"
     CDS             complement(9468..10946)
                     /gene="astD"
                     /locus_tag="ACIUS0_002843"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGB0220522.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10943..11977)
                     /gene="astA"
                     /locus_tag="ACIUS0_002844"
     CDS             complement(10943..11977)
                     /gene="astA"
                     /locus_tag="ACIUS0_002844"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGB0220523.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11974..13194)
                     /gene="astC"
                     /locus_tag="ACIUS0_002845"
                     /pseudo
     CDS             complement(11974..13194)
                     /gene="astC"
                     /locus_tag="ACIUS0_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13640..14446
                     /gene="xthA"
                     /locus_tag="ACIUS0_002846"
     CDS             13640..14446
                     /gene="xthA"
                     /locus_tag="ACIUS0_002846"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGB0220524.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14613..15323
                     /locus_tag="ACIUS0_002847"
     CDS             14613..15323
                     /locus_tag="ACIUS0_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGB0220525.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15328..16005
                     /gene="ydjY"
                     /locus_tag="ACIUS0_002848"
     CDS             15328..16005
                     /gene="ydjY"
                     /locus_tag="ACIUS0_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGB0220526.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16020..16727
                     /locus_tag="ACIUS0_002849"
     CDS             16020..16727
                     /locus_tag="ACIUS0_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGB0220527.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16727..17275
                     /locus_tag="ACIUS0_002850"
     CDS             16727..17275
                     /locus_tag="ACIUS0_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGB0220528.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17285..17611
                     /locus_tag="ACIUS0_002851"
                     /pseudo
     CDS             17285..17611
                     /locus_tag="ACIUS0_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4367 a         4684 c         4307 g         4286 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tacggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aatatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttgacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctcgcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatccgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattct acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgagt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acacgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccaccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca acacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgcagtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc agtgcttccc
     8221 gcccttcgcg cagcaattgc gggtcggcca gatcggcagc agtaagacga tcgcggtagt
     8281 aacgatccac ccagtcattg agcgcattaa acaaggtatc gttcatcatc accgccggat
     8341 tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac gccgggccgc
     8401 cgccattcgc catgctttca cgtaagtcaa agacttttag ttcgctaatc gggttgtcag
     8461 cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac tcctgaggca
     8521 gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac agataggtag
     8581 acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata aaaccgttca
     8641 cccgcgtgcc agtaactgtg actggcgagc gaacgcctgt tggtggcaaa acagcacctg
     8701 gcggttactc acggcaatca cgtcattatg aaaaacgctc tggtcgataa cgtccgggtt
     8761 ttgctgggcg aaaatcacct gttggggatt cacctgatta agccttgcca ccgcctcgct
     8821 ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg gtatcattgc cttcttctcg
     8881 cccgtagaca aaaagtagca tacccggttc accgtaatga ccgccgagac gattgtggtt
     8941 tgccgccccc tcatcaccga gcaacgctac ctgcggcaac gccgaatgga cgctaaattt
     9001 ctcttcgtcc ttaaaaatcg cttttaacag cgattcagtg acgggcgctt ccagcgaacg
     9061 gtgaaattta ttgttcaggt tggcaacggt gagatgcact ttgccatcca gcgtatcggc
     9121 agatggcgcg atcgttgccg cattggctac ccacattggc gaagcggaac tgacgctgga
     9181 aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc tgctcatcgc taccactgaa
     9241 tcccaactga cgcagcaccg gaataaacgg acgctcgtgc ggcggaatca cggcctgggg
     9301 gaatcccgca tcggcaaggg ctttcatttt cagtaagccc tgttttgccg ccagtcgcgg
     9361 gttagacacc tgaaaacggt gacgggtaga ggcttcatta ccaaacgaca ggcccgcgta
     9421 atgatgcgtc agccctacca gcccgtcgaa attaacttcc caggcgttca tcgcaccacc
     9481 tcatcggaaa aatccagccc ggggttaagc gtggcgggca atgttaacga gtccgactcc
     9541 aggctcgcca taggccatgc gcagtaatct gcggcatacc aggcgctggg gcgatggtta
     9601 ccggaagcac caatgccgcc gaatggggcg gtactggcag caccagtaag cggtttgttc
     9661 cagttaacaa tccccgcccg cgcctccagc aacagttgat cgaatttttc ccgctcgggg
     9721 gaaaccagac cgcaaaagag tccgaagcga gtgttgttcg ccatccgaat cgcctcatca
     9781 aaagtatcat aacgccagac gcgcaaaaac ggcccgaaca cctcttcatc cggcacaccg
     9841 ccaacgcccg tcatttcaat gatccctggt gtcagcaacg atgtccctgc ttgtaataag
     9901 cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt gctgccaggc agtaaccacc
     9961 tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg gctgcggttc gtcatcccag
    10021 ttgcccggcg ttaatcgctg gctgacggca accagactag caagaaacgc atcgccctgc
    10081 gctccgcttt tgagcaataa acggcgggcg caggtgcagc gttgcccggc tgtgacaaac
    10141 gccgactgaa tggtcagatg gacagccgcg tcgatatccg ccacctcatc gataattagc
    10201 ggattattac cgcccatctc aagggcgaga attttctccg gctgaccgga gagctggcga
    10261 tgcagctggt agccggtatt ggcgctaccg gtaaacagca aaccgtcgag atcctccagc
    10321 gcactcagcg cctgacccgt ttcacgcccg ccctgcacca ggttcagcac gcccggcggc
    10381 aagccagcct gctgccataa acgcattacc gcttcgccac tccacggtgt cagttcgctg
    10441 ggtttaaaga taatggtgtt acctgccagc aatgccggaa cgatatgtcc gttcggcaaa
    10501 tgaccaggga aattatacgg cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg
    10561 ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg tacgaacgtg atacgcctta
    10621 attgatatcg cgattttatt gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc
    10681 ttacccgttt ctctggcaat aatcgcggtt aattcggctt tattgctttc cagcaatccg
    10741 gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga gccgcgccca gcgcggaaac
    10801 gccgcacggg ctgcccgaca agcctgttcg acctgagcgg catcggcatc attgccttgc
    10861 cataacacct cgcccgatac cggattacgc ttcacacgcg atgcgccctg gcccgttatc
    10921 cagtcaccgt taatccataa agtcatgctg ttttctcctc tgcgcacagg cgcaccagac
    10981 gaacgcgatc ccctgcgtgg catttgaggg catccagttg tgcggcggtt aaaatcaaac
    11041 gctcggttgc cggatcggta cgcgccagca ccacgcggaa atggtgataa ttttcattgg
    11101 cgaccaggca ggccggaaaa tcgccctgcg caggctgccc ttctgccact tccaccagcc
    11161 gacttttacg gatggcgcgc acgcggtcga tgtcacactc aagcgttggc ccgccgtcga
    11221 agatgtcgat atagttgcgg taacgaaaac cttctttctc cagcaccgcg cgggcaggcg
    11281 cggtttgcgg atgtacctga ccgatgacgt cctgggcttc ctgggataaa aagtgggtat
    11341 agatcggatg tttcggcatc agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa
    11401 aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc gaggctttgc cagaacggtg
    11461 aatagccgtg ttcgtcaatc accccgcgca tttcagcaac cactttgtca ttaaacttgt
    11521 cgcgaaaagc cgccataaac ataaagcgcg atttcgacag caaatagccg ttgccctctt
    11581 tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct gctgccggtg tgatcgttac
    11641 taagaaacag cgtcggcaat gcgttataga cattcagctc ttttgaggcg tgaaccaacg
    11701 tgccgacgcg atagttgtac cagggatcgt tcagcccaac cgccacctca atagcgcaaa
    11761 tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa cacatagccc tgctcgcttt
    11821 tgggcagttc accttgccag gttttgattg ccctttcgat acgcgccgaa agcgtggctt
    11881 cattggcggg aagcgacgtc aggccgccgc ccgttttgct ggcaagctgc atcagcgccg
    11941 agacatctga gcgctcaacg ggacggatga ccatcatgat gaacctcggc taacaaagtg
    12001 ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag
    12061 cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt accatcacgc ctgctttcgc
    12121 cgcttccaga gagatctgtt tcgcttgccc ggcgtaatcg gcattcagta cacagccaat
    12181 caacaaacct aagccgcgaa cttcactgaa caaaccatag cggtgattaa tagtattaag
    12241 acgctcaaca aaccagtcgt gacgctgttt aacgccatta agcatctctg gtgtgttgat
    12301 gagctccagc actttgcctg ctaccgccga ggccagcgga ttaccgccat aggtggtgcc
    12361 atgagtgcca acggtcatca cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg
    12421 gaaaccgccg cccagcgctt tggcggtggt taacagatcg ggcgtcacgc cgtagtgcat
    12481 ataggcatac agctcaccag tgcgcccgac gccggtttgt acctcatcaa aaatcagcaa
    12541 ggcattgtgg cggtcacaca attcacgcag accttgtaaa aacgcgttgc tggctggcac
    12601 cacaccgcct tcgccctaga tgggctcgac aatcaccgca caggtggcgt cgtcaatcag
    12661 cgcgctggca gagttaatat cgttatatgc agcatgacga atatccgccg gcagtggcgc
    12721 aaaatcctgt gaataggctg gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg
    12781 aaacgcattt ttgaacgcca cgatgccact cttatggctg ccgtagcggt cgtgagcgaa
    12841 tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca ccggagttac aaaagaagac
    12901 gcgatcggca aacgtggcgt cgatcaattt tttcgccagt cgcagtaccg gctcgttggt
    12961 gtaaccgttg ccggtatgcc agaacttact cgcctgttcg ttcagcgctt cacgcagttc
    13021 cggatgcgca tggcccagcg cgttcaccgc aatgccaccc gcgaagtcga tatactcttt
    13081 cccctgctga tcccacaagc gcgaaccttc gccacgtacc ggtataaagg gtgccggagc
    13141 gtaaacaggt atcatccatt catcaaagtt ttcacgcgta attggctgag acatagcgac
    13201 ctctacagta aataattcgt tatttgtatg ttaacaataa gtaatgtttg cgctgtaaat
    13261 gtagattgca gggttcgtgc cagccagtga taaaagtgca taaacggcgg aggctaactg
    13321 gaaatcaagg agttataacc aaaccatatg catttaaagt gcatataaag tgaatacgtt
    13381 tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt atgcagaagt aaaatataat
    13441 tctagaattg tgatcattga cgaaatttac tggaaattac tgcgccattc tgacgcagcg
    13501 cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc attaacaacc atcgatcaaa
    13561 tcacttaaca acaggcggta agcaacgcga aattctgcta ccatccacgc actctttatc
    13621 tgaataaatg gcagcgacta tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag
    13681 acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga
    13741 gacaaaagtt catgacgata tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt
    13801 gttttatcac gggcagaaag gccattatgg cgtggcgctg ctgaccaaag agacgccgat
    13861 tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg cagcggcgga ttattatggc
    13921 ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga
    13981 aagccgcgac catccgataa aattcccggc aaaagcgcag ttttatcaga atctgcaaaa
    14041 ctacctggaa accgaactca aacgtgataa tccggtactg attatgggcg atatgaatat
    14101 cagccctaca gatctggata tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac
    14161 cggtaaatgc tctttcctgc cggaagagcg cgaatggatg gacaggctga tgagctgggg
    14221 ggtggtcgat accttccgcc atgctaatcc gcaaacagca gatcgtttct catggtttga
    14281 ttaccgctca aaaggttttg acgataaccg tggtctgcgc atcgacctgc tgctcgccag
    14341 ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac tatgaaattc gcagcatgga
    14401 aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc cgctaattta gcagctctcc
    14461 tggctcaaac tgggtcagga gaattaacct tgagaaaaat caacaaactg tcagtaatga
    14521 tttgttgcct gccgtccttt gttataccgt cactgcgttt ttagttgtct gaccacttct
    14581 ctattatcaa gtttgatata ggaaactcca cgatgaacgc tgagcgtaac tttctgtttg
    14641 cctgtcttat ttttgcgctg gtcatttacg ctatccacgc tttcggttta ttcgatctgc
    14701 tcaccgattt accccactta cagacactca ttcgccagag cggattgttc ggttatagcc
    14761 tctatattct gttattcatc aatgcccccc tctttctgtt accaggaagc atactggtga
    14821 tcgctggagg aatagttttt ggcccgctct tagggacaca actctcatta attgccgcaa
    14881 cgctggcctc ctcgtgctca ttcctgctgg cgcgctggct gggacgtgat ttactgctga
    14941 aatacgtggg ccatagccat accttccagg ccattgaaaa aggcattgcg cgtaacggta
    15001 ttgattttct tattctgacc cgcttaatcc cgttgtttcc ttacaatatt caaaattacg
    15061 cttacggatt caccaccatc gccttctggc cttataccct tatttcggca ctcacgaccc
    15121 tgcccggtat tgttatttat accgtgatgg caagcgatct cgccaatgaa ggcattacgc
    15181 tgcgctttat tttacaactc tgtctggcgg gcctggcgct gtttattctc atccagctcg
    15241 caaaactcta cgcccgacac aaacatgtgg atctgtctgc ttcgcgccgc agcccactta
    15301 ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt attcagtgtc atggaaaaaa
    15361 ggactggctg cactctgctt actggctgtt gccgggctta gcggctgcga tcaaaaagag
    15421 aatgcggcgg caaaagtgga atacgacgga ctttcgaaca gccaaccgct gcgcgtcgat
    15481 gccaataacc atacggtaac catgctggtg caaattaatg gtcgtttcct caccgacgac
    15541 actcgtcacg gtattgtgtt taaagatggc tccaacggac ataaatcgct gtttatggct
    15601 tatgcgcccc cgaaagcatt ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa
    15661 aacatgacga tggataataa agaaacgact catgtcacag gcagcaaact ggatatttcg
    15721 gtcaactggc aaggggcggc aaaagcgtat tccttcgatg aagtgattgt tgacagtaat
    15781 ggcaagaaac tggacatgcg ctttggcggt aatttaacgg cagcagaaga gaagaaaacg
    15841 ggttgcctgg tgtgtctgga tagctgcccg gtcggcatcg tcagcaatgc aacatacact
    15901 tatggtgcgg tggaaaaaca cggcgaagtg aaattcaaag gcaatgcctc agttctcccg
    15961 gcggataaca cgctggcaac ggttaccttt aaaatcaccg aataaagcca agataaagga
    16021 tgatgaagat acaatcgcgt aaaatctggt actaccgtat aaccctcatc atcctgttgt
    16081 tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg
    16141 ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg ctttattcag tcttacggcg
    16201 cactggcggc agttgtctcg ttcttgttga tgattttaca ggccattgct gcaccgctac
    16261 ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc
    16321 tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg
    16381 gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct tgacagtatg gacggctttt
    16441 tcactcgcta cggcaaacac accatcctgg tctgtcggtt attgcctttt gtccctttcg
    16501 atccaatcag ctatgctgcc ggtctgactt caatacgttt tcgctcgttt tttatcgcca
    16561 ccgggcttgg tcaattaccg gcgactattg tttattcctg ggcaggcagc atgttaacag
    16621 gcggtacttt ctggtttgtc accgaactgt ttattctgtt tgccctgacc gtggtgattt
    16681 ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa tgactgatgg gtttaccgcc
    16741 gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg ctgcatcgtc gccattacgg
    16801 cgaggtgcta agcccgattc gctggtgggg gcggatcccg tttatctttt atttggtgtc
    16861 aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc gatccggtag tgcgatcgct
    16921 tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt tgcgtggata tcaccagtat
    16981 gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg gcagtggctg actggcggca
    17041 aagcccgctc tttagcgatg aagaacggct ggcgctggag tacgccgaag ccgcaagcgt
    17101 cacaccgcca acagtcgatg atgccctgcg tacccgactg gctgcgcatt ttgacgctca
    17161 ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac ctgtcggctc gttttaattc
    17221 tgccatggac attcccgctc aggggctgtg ccgtattccg gaaaaacgtt cttaaggagg
    17281 gaagatgcgc cattgtgggt ggttgctggg attgttatcg ctgttttctt tggcaacaca
    17341 tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc
    17401 ctggggcggc gataccgcaa ttaaccgcta tctcgactgg gttagcggcg agatgaaaac
    17461 ccattacgct ataaacctga agattgttcg tctcgcggat gccgcagacg cggtgaagcg
    17521 cattcagacc gaagctgctg ccggtcgtaa aacgggcggc tcggtggatc tgctctgggt
    17581 gaacggcgaa aacttccgca ccttaaaaga gtaatgccag tcagttaagc aactgactgg
    17641 catt
//
LOCUS       DBBBSB010000087        17428 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.087, whole genome shotgun sequence.
ACCESSION   DBBBSB010000087 DBBBSB010000000
VERSION     DBBBSB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.087"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACIUS0_002852"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACIUS0_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGB0220529.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACIUS0_002853"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACIUS0_002853"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGB0220530.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACIUS0_002854"
     CDS             2826..3980
                     /locus_tag="ACIUS0_002854"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGB0220531.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACIUS0_002855"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACIUS0_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGB0220532.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMIFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACIUS0_002856"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACIUS0_002856"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGB0220533.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACIUS0_002857"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACIUS0_002857"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGB0220534.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACIUS0_002858"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACIUS0_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGB0220535.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACIUS0_002859"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACIUS0_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGB0220536.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACIUS0_002860"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACIUS0_002860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGB0220537.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACIUS0_002861"
     CDS             11296..11511
                     /locus_tag="ACIUS0_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGB0220538.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACIUS0_002862"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACIUS0_002862"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGB0220539.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACIUS0_002863"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACIUS0_002863"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGB0220540.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACIUS0_002864"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACIUS0_002864"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGB0220541.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACIUS0_002865"
     CDS             complement(16247..16786)
                     /locus_tag="ACIUS0_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGB0220542.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACIUS0_002866"
     CDS             complement(16802..17320)
                     /locus_tag="ACIUS0_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGB0220543.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4051 a         4323 c         4835 g         4219 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgatct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgc
//
LOCUS       DBBBSB010000088        17091 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.088, whole genome shotgun sequence.
ACCESSION   DBBBSB010000088 DBBBSB010000000
VERSION     DBBBSB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.088"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..510
                     /locus_tag="ACIUS0_002867"
     CDS             <1..510
                     /locus_tag="ACIUS0_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGB0220544.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACIUS0_002868"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACIUS0_002868"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGB0220545.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACIUS0_002869"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACIUS0_002869"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGB0220546.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACIUS0_002870"
     CDS             complement(2878..3075)
                     /locus_tag="ACIUS0_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGB0220547.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACIUS0_002871"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACIUS0_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGB0220548.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACIUS0_002872"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACIUS0_002872"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGB0220549.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACIUS0_002873"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACIUS0_002873"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGB0220550.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACIUS0_002874"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACIUS0_002874"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGB0220551.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIIAGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACIUS0_002875"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACIUS0_002875"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGB0220552.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACIUS0_002876"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACIUS0_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACIUS0_002877"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACIUS0_002877"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGB0220553.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACIUS0_002878"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACIUS0_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGB0220554.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACIUS0_002879"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACIUS0_002879"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGB0220555.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACIUS0_002880"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACIUS0_002880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGB0220556.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACIUS0_002881"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACIUS0_002881"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGB0220557.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACIUS0_002882"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACIUS0_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         3993 a         4866 c         4443 g         3789 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgcaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta a
//
LOCUS       DBBBSB010000089        16363 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.089, whole genome shotgun sequence.
ACCESSION   DBBBSB010000089 DBBBSB010000000
VERSION     DBBBSB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16363
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.089"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACIUS0_002883"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACIUS0_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACIUS0_002884"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACIUS0_002884"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGB0220558.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACIUS0_002885"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACIUS0_002885"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGB0220559.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACIUS0_002886"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACIUS0_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACIUS0_002887"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACIUS0_002887"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGB0220560.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACIUS0_002888"
     CDS             complement(5877..6218)
                     /locus_tag="ACIUS0_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGB0220561.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACIUS0_002889"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACIUS0_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGB0220562.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACIUS0_002890"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACIUS0_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGB0220563.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACIUS0_002891"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACIUS0_002891"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGB0220564.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACIUS0_002892"
     CDS             12897..14240
                     /locus_tag="ACIUS0_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGB0220565.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACIUS0_002893"
     CDS             complement(14302..14508)
                     /locus_tag="ACIUS0_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGB0220566.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACIUS0_002894"
     CDS             14833..15387
                     /locus_tag="ACIUS0_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGB0220567.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>16363)
                     /locus_tag="ACIUS0_002895"
     CDS             complement(15450..>16363)
                     /locus_tag="ACIUS0_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220568.1"
                     /translation="FIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWVSNYDSLLTL
                     RESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLRGSGFEYEEI
                     PITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQAFGDVSFGY
                     ALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGD
                     ERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICE
                     KYKSTIEAFKKEQLALPVLVRCKNSET"
BASE COUNT         3919 a         4451 c         3985 g         4008 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gatcgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aat
//
LOCUS       DBBBSB010000090        16096 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.090, whole genome shotgun sequence.
ACCESSION   DBBBSB010000090 DBBBSB010000000
VERSION     DBBBSB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.090"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..588
                     /locus_tag="ACIUS0_002896"
     CDS             <1..588
                     /locus_tag="ACIUS0_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGB0220569.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="ACIUS0_002897"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="ACIUS0_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGB0220570.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="ACIUS0_002898"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="ACIUS0_002898"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGB0220571.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="ACIUS0_002899"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="ACIUS0_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACIUS0_002900"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="ACIUS0_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGB0220572.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="ACIUS0_002901"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="ACIUS0_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGB0220573.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="ACIUS0_002902"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="ACIUS0_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGB0220574.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="ACIUS0_002903"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="ACIUS0_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGB0220575.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="ACIUS0_002904"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="ACIUS0_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGB0220576.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="ACIUS0_002905"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="ACIUS0_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGB0220577.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="ACIUS0_002906"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="ACIUS0_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGB0220578.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="ACIUS0_002907"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="ACIUS0_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGB0220579.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="ACIUS0_002908"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="ACIUS0_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGB0220580.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="ACIUS0_002909"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="ACIUS0_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGB0220581.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACIUS0_002910"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="ACIUS0_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGB0220582.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="ACIUS0_002911"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="ACIUS0_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGB0220583.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="ACIUS0_002912"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="ACIUS0_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGB0220584.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="ACIUS0_002913"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="ACIUS0_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGB0220585.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="ACIUS0_002914"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="ACIUS0_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGB0220586.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="ACIUS0_002915"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="ACIUS0_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGB0220587.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="ACIUS0_002916"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="ACIUS0_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGB0220588.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="ACIUS0_002917"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="ACIUS0_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGB0220589.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="ACIUS0_002918"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="ACIUS0_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGB0220590.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="ACIUS0_002919"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="ACIUS0_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGB0220591.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="ACIUS0_002920"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="ACIUS0_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGB0220592.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="ACIUS0_002921"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="ACIUS0_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGB0220593.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACIUS0_002922"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="ACIUS0_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGB0220594.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="ACIUS0_002923"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="ACIUS0_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGB0220595.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="ACIUS0_002924"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="ACIUS0_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGB0220596.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="ACIUS0_002925"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="ACIUS0_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGB0220597.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="ACIUS0_002926"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="ACIUS0_002926"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGB0220598.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="ACIUS0_002927"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="ACIUS0_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGB0220599.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3784 c         4292 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DBBBSB010000091        15933 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.091, whole genome shotgun sequence.
ACCESSION   DBBBSB010000091 DBBBSB010000000
VERSION     DBBBSB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.091"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            449..1507
                     /gene="citC"
                     /locus_tag="ACIUS0_002928"
     CDS             449..1507
                     /gene="citC"
                     /locus_tag="ACIUS0_002928"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGB0220600.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1522..1818
                     /gene="citD"
                     /locus_tag="ACIUS0_002929"
     CDS             1522..1818
                     /gene="citD"
                     /locus_tag="ACIUS0_002929"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGB0220601.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1815..2723
                     /gene="citE"
                     /locus_tag="ACIUS0_002930"
     CDS             1815..2723
                     /gene="citE"
                     /locus_tag="ACIUS0_002930"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGB0220602.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGDNAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2734..4266
                     /gene="citF"
                     /locus_tag="ACIUS0_002931"
     CDS             2734..4266
                     /gene="citF"
                     /locus_tag="ACIUS0_002931"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGB0220603.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4270..4820
                     /gene="citX"
                     /locus_tag="ACIUS0_002932"
                     /pseudo
     CDS             4270..4820
                     /gene="citX"
                     /locus_tag="ACIUS0_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4795..5673
                     /gene="citG"
                     /locus_tag="ACIUS0_002933"
     CDS             4795..5673
                     /gene="citG"
                     /locus_tag="ACIUS0_002933"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGB0220604.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5724..7186
                     /gene="citT"
                     /locus_tag="ACIUS0_002934"
                     /pseudo
     CDS             5724..7186
                     /gene="citT"
                     /locus_tag="ACIUS0_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7300..8106
                     /gene="rna"
                     /locus_tag="ACIUS0_002935"
     CDS             7300..8106
                     /gene="rna"
                     /locus_tag="ACIUS0_002935"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGB0220605.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8335..8745
                     /gene="rnk"
                     /locus_tag="ACIUS0_002936"
     CDS             8335..8745
                     /gene="rnk"
                     /locus_tag="ACIUS0_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGB0220606.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8883..8972
                     /gene="yldA"
                     /locus_tag="ACIUS0_002937"
     CDS             8883..8972
                     /gene="yldA"
                     /locus_tag="ACIUS0_002937"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGB0220607.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8976..10214)
                     /locus_tag="ACIUS0_002938"
     CDS             complement(8976..10214)
                     /locus_tag="ACIUS0_002938"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGB0220608.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10435..10863
                     /gene="uspG"
                     /locus_tag="ACIUS0_002939"
     CDS             10435..10863
                     /gene="uspG"
                     /locus_tag="ACIUS0_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGB0220609.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10984..12549)
                     /gene="ahpF"
                     /locus_tag="ACIUS0_002940"
     CDS             complement(10984..12549)
                     /gene="ahpF"
                     /locus_tag="ACIUS0_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGB0220610.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12794..13357)
                     /gene="ahpC"
                     /locus_tag="ACIUS0_002941"
     CDS             complement(12794..13357)
                     /gene="ahpC"
                     /locus_tag="ACIUS0_002941"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGB0220611.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13729..14475
                     /gene="dsbG"
                     /locus_tag="ACIUS0_002942"
     CDS             13729..14475
                     /gene="dsbG"
                     /locus_tag="ACIUS0_002942"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGB0220612.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14685..15587
                     /locus_tag="ACIUS0_002943"
     CDS             14685..15587
                     /locus_tag="ACIUS0_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0220613.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15734..15933
                     /locus_tag="ACIUS0_002944"
                     /pseudo
     CDS             15734..15933
                     /locus_tag="ACIUS0_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3958 a         4117 c         3854 g         4004 t
ORIGIN      
        1 aaagatacgc agcggaaatg ccagtctttg gaaaaatgca aactgtttat tctcgttaag
       61 ctgcaacatt gttttccttg cgggttgtcc cgagcctctt tgacggatgt aattaaaatg
      121 ttgaatcaaa atacatagat attaattatg atcgtggcaa attttattca ttaaatcaat
      181 taaatcactt aaacctgcga gtcatttcgt aattacatta attaaaaaac ttaaaaccat
      241 taaataaata aaaaaccact aactctatgt gaaataaatc aaaatttcac gccgaaatac
      301 tccttaggat gtatagcaaa aggagaaaaa gatatacctc gatcaccccc ctttctccca
      361 agtgaaaata aaaggttatc agtttgcaac attgaacaac attcgttgca aatcgataac
      421 aacatgcacc ttcaggatac tatttattat gttcggcaat gatattttca cccgcgtaaa
      481 acgttcagaa aataaaaaaa tggcggaaat cgcccaattc ctgcatgaaa atgatttgag
      541 cgttgacacc acagtcgaag tatttattac cgtaacccgc gatgaaaagc ttatcgcgtg
      601 cggtggaatt gccggaaata ttattaaatg cgttgctatc agtgaatccg ttcgcggtga
      661 aggactggcg ctgacattag ccactgaact gataaacctc gcctatgagc ggcacagcac
      721 gcatctgttt atttatacca aaaccgaata cgaggcgctg ttccgccagt gcggtttttc
      781 cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa aacagcgcca cgcgactgaa
      841 acgctatgcc gaatcgctga aaaaatttcg tcatccaggg aacaagattg gctgcattgt
      901 gatgaacgcc aatcccttta cgaatggtca ccgttatctg attcaacaag ctgcggcaca
      961 gtgcgactgg ttgcatctgt ttttagtcaa agaagattct tcacgcttcc cctatgaaga
     1021 ccggctggat ctggtgttaa aaggcaccgc cgatattcca cgcctgactg tgcatcgcgg
     1081 ctcagaatac atcatctccc gcgctacgtt cccttgctac ttcattaaag aacagagcgt
     1141 cattaaccat tgttacaccg aaattgatct gaagattttc cgtcagtacc tcgctcccgc
     1201 gcttggtgta actcaccgct ttgtcggtac tgaacccttt tgtcgcgtta ccgcccagta
     1261 caaccaggat atgcgctact ggctggaaac gccgactatc tccgcagcgc ccatcgaact
     1321 ggttgaaatt gagcggctgc gttaccagga gatgccgata tccgcttccc gggtacgtca
     1381 actgctggcg aaaaacgatc tcacggctat cgcgccgctg gtccctgcag tcacgctgca
     1441 ttatttgcag aacctgcttg agcactcccg ccaggacgcg gcagctcgtc aaaagacccc
     1501 cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg ccgttgcagg cacccttgag
     1561 tctggggatg tgatgatacg catcgcccca ctcgatacgc aggatatcga cctgcaaatc
     1621 aatagcagcg ttgagaaaca gtttggcgat gcaattcgca ccaccattct ggacgttctc
     1681 gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg acaaaggcgc actggactgc
     1741 attttacatg cacgactgga agccctgctg gcacgcgcca gcggtatccc ggctctgcca
     1801 tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg taaaactcgc acccgccgca
     1861 gcatgttgtt tgtgcctggt gacaatgccg cgatggtcag caactccttc atctacccgg
     1921 ctgatgccct gatgtttgac ctcgaagact ccgtggcatt acgtgaaaaa gacaccgccc
     1981 gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg cgatattgaa accattgtgc
     2041 gtgtcaacgc gctggattcc gaatggggtg ttaacgacct ggaagccgtc gttcgcggtg
     2101 gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca ggatgttctg gatattgaaa
     2161 aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc cggcagcacc ggcctgctgg
     2221 cggcgattga atctccgctg ggcattaccc gcgcagtgga aatcgctcac gcttccgagc
     2281 gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg caacctgcgt acagaacgct
     2341 ccccggaagg aactgaactg ctgttcgcac gctgttccat tttgcaggcc gcgcgctctg
     2401 cgggtattca ggcgttcgat accgtctatt ccgacgctaa caacgaagcc ggattcctgc
     2461 aagaagccgc ccacatcaaa cagctgggct ttgacggcaa atcgctgatc aacccgcgtc
     2521 agattgatct gctgcacaac ctctacgcac cgacccagaa agaagtggat cacgcccacc
     2581 gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct cggcgtggtt tccctgaacg
     2641 gcaagatggt ggacggtccg gttatcgatc gcgcccgtct ggtgctctcc cgtgcagaac
     2701 tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc agaaaattga acaatctcaa
     2761 cgacaagaac gggtagcggc ctggaatcgt cgcactgaat gcgatcttgc cgctttccag
     2821 aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg atcgcaaact gtgcgccaac
     2881 ctggaagaag cgattcgtcg ctcaggttta caggacggca tgacggtttc cttccatcac
     2941 gctttccgtg gcggtgacct gaccgtcaat atggtgatgg acgtcatcgc gaagatgggc
     3001 tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt gccatgcgcc gctggtagaa
     3061 cacattcgcc agggcgtggt tacccgcatt tatacctctg gcctacgcgg tccactggcg
     3121 gaagagatct cccgtggtct gctggcggaa ccggtgcaga tccactctca cggcggtcgt
     3181 gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg ctttcctcgg cgtcccgtcc
     3241 tgtgatgaat tcggtaatgc caacggctac accggtaaag cctgctgcgg ctccctcggc
     3301 tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga tgcttaccga agaactgctg
     3361 ccttatccgc ataatccggc aagcattgag caagatcagg ttgatttgat cgtcaaagtt
     3421 aatcgcgttg gcgatgctgc aaaaatcggc gctggtacta cccgtatgac cactaacccg
     3481 cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg tcaactctgg ctacttcaaa
     3541 gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc tggcggtaac ccgtttcctg
     3601 gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg ccctcggcgg tattaccgcg
     3661 acgatggttg atctgcacga aaaaggtctg atccgcaaac tgctggatgt gcagagcttt
     3721 gacagccatg ctgcgcaatc gctggcccgt aaccccaatc acatcgaaat cagcgccaac
     3781 cagtacgcta actggggttc gaaaggcgca tcggttgatc gtctcgacgt ggtggtactg
     3841 agcgcgctgg aaattgacag gcagttcaac gttaacgtgc tgaccggttc tgacggcgta
     3901 ctgcgtggcg cttccggtgg tcactgcgat accgcgattg cctctgcgct ttccatcatc
     3961 gtcgcgccac tggtacgcgg tcgtattccg actctggtgg ataacgtgct gacctgcatc
     4021 accccgggct ccagtgtcga tattctggtc acagaccacg gtatcgcagt taacccggca
     4081 cgtccggaac tagcagaacg tctgcaggaa gcgggcatta aagtggtttc cattgagtgg
     4141 ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc cgattgaatt caccgaccgc
     4201 gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg atgttgtgca tcaggtgaag
     4261 gaataagcca tgcacctgct tcctgaactc gccagccacc atgcggtgtc aattcccgag
     4321 ctgctcgtca gccgggatga aaggcaagca cggcaacacg cctggctcaa gcaccatcct
     4381 gttccactgg tctcctttac cgtggttgcg cctgggccga ttaaagacag cgaggtcaca
     4441 cgccgaattt ttaatcatcg cgtgacagcc ttgcgtgcct tagccacaaa gcagggctgg
     4501 caaattcagg agcaggctgc actggtttcc gccagcgggc cggagggcat gttgagcatt
     4561 gccgccccgg ctcgcgacct caagctcgcc accattgagc ttgaacatag tcatcctctc
     4621 gggcggttat gggatatcga tgtcctgacg cccgaaggcg aaattctctc ccgccgcgac
     4681 tattcactgc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc tgcgcgcgtg
     4741 gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg aacgatgtcg
     4801 atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga tgagtacgcc
     4861 ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc aggcctcgtg
     4921 gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca ccgcagcgcg
     4981 ctggcgattc agggctggtt accccgtttc attgaatttg gtgcctgtag tgcagaaatg
     5041 gcaccggaag cggtactcca cggattacgc ccaattggta tggcttgcga aggtgatatg
     5101 ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt agggctgcta
     5161 tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg ttacgccaac aaccgtttgt
     5221 tctacggcgg caagtttctg ccgtggcctg accgatcgcg aactgcgtac caataattca
     5281 cgactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa
     5341 gccgaagcag gttatccact ggtgatcaac cacgccttgc cgcattacct cactctgctg
     5401 gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact gatggctacc
     5461 aacggcgata ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc
     5521 gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccaccgatct cgattatctc
     5581 cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag tgccgacctg
     5641 ctgatcctta cctgtttttt agcacagatt taattattta agcacttgat aaatttggaa
     5701 atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaactattgg
     5761 ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt atgccgccgc
     5821 aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa
     5881 ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc
     5941 tgctctttga tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga
     6001 aatggggcct ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt
     6061 ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat
     6121 tcatggacaa acgcacgctg acgttgggtt atgcgattgt cattatcgac attctgctgg
     6181 caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa
     6241 acctgccgcc gttgtttaaa tcattcccga acgatccgtc cgcgcgtcgt attggcggct
     6301 atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg
     6361 cggaccaaac gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg
     6421 gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg
     6481 gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc
     6541 gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct
     6601 tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt
     6661 tggtctgctg gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga aagacattac
     6721 ccgctataac agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa
     6781 cggcctgact cgttctggtt ttattgactg gttcgccggt accatgagta cgcacctgga
     6841 aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta
     6901 cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg
     6961 taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg
     7021 tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg
     7081 ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc
     7141 tatgttgctg ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac
     7201 gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg cgggcttttt tatgatttaa
     7261 tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctgccgtaac
     7321 gccgcgttgc tcgcggtttc tctgcttccc ttatcttctg ccaacgccgt agcgttgcag
     7381 gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt
     7441 tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa
     7501 acgaccaaca aaactgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg
     7561 gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca
     7621 atcccgaatc taccagaagc acgcgccagc cgaatgtgtt catcgccgga aaccgggtta
     7681 tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc
     7741 ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac
     7801 ttcggtacga tggtacgcct gaatcaagaa attaaagaga gcgaagccgg aaaattcctt
     7861 gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc
     7921 tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg
     7981 actgaaattc agatctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca
     8041 ttcttgccac aacctcaccc aggtaactgt ggcaaaacct ttgtgattga taaagcgggt
     8101 tattaatcct ctgatttaca aaacacgcta attcgtcttc cataaccatg ccgattaaga
     8161 attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga
     8221 gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt
     8281 tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt aaaaatgtcc
     8341 agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct gctggagcaa
     8401 cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa
     8461 atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg ggttaaattc
     8521 cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc
     8581 gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct ggggctgcgc
     8641 gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct tgaagtgctg
     8701 gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca tctgcacaag
     8761 cgataactcg ctttgcccag aggatgcact cgcatcctct ttacgcaaaa tcagcaattt
     8821 ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt gtgggaataa
     8881 ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg attatcgtca
     8941 tggctgtcct ttacctcgaa aaccactcct gacgatcata ttgttccccc cggcatcgca
     9001 ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc gggtaccaga
     9061 atcaccttac ggcactcctc ttcacgtttt tcgaaaatct catatccccg ggcggcctct
     9121 tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt ctcaattaac
     9181 ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa cgtcaaccct
     9241 ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac gcccggtacg
     9301 ctaacaatgc cgccacgcct gaccgccgca atacactggc gcaacgcttt accgctgctg
     9361 ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc ttcaaaaccg
     9421 acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat aattgactgt
     9481 gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc agcggcgaaa
     9541 cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag caaccgtgca
     9601 caggcgattg tcaacaatcc cacaggacca gcaccataga ctgcaacgct tgaaccttgt
     9661 tggatctgca cattttttgc tgcctgccat gccgttggca gaatatcaga aaggaaaagg
     9721 gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc tttcgggacg
     9781 cggacatatt ccgcctgccc gccaggaacg ccaccataca ggtgactata accaaacaat
     9841 gccgcgggag caggtatctg ttttttattg agcgcagcgc ctttacccgc attggtattt
     9901 tcgcaggcgg catattgctg taatcgacag aaaaaacagt cgccacaagc aatgacgaac
     9961 ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt ttcaacgact
    10021 tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat tttgcctcga
    10081 taaagatgga ggtcagagcc acagatcgcc gttaccgtaa tacgcagaat aatatcatct
    10141 gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg gccgtgataa
    10201 gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct ttaagggtag
    10261 ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattccta gtgttcagtg
    10321 agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa tatcagggat
    10381 aaatattata atcatggaga atgatttgtt catgattaac agggagaaag ggttatgtat
    10441 aaggcaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa agctgttcgc
    10501 cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt actacccggg
    10561 tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga agagcatctg
    10621 caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat cgatccttcc
    10681 cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga gttggcagaa
    10741 gaactggggg ctgatgttgt agttatcggt tctcgcaacc catcgatttc gacccatctg
    10801 ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact ggtcgttcgc
    10861 taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc tttttaatgc
    10921 cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga atcttacttc
    10981 ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg ctttggcacc
    11041 ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac ccgctgcgaa
    11101 cacgcctttg acgttggttt cacatttcgc atcaatgata atctcgccca tgcggttacg
    11161 ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga caaaaatacc
    11221 ggccagttcg atgttgtgaa tattgccgct gacacgatct cgatattcca gaccaacgac
    11281 tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt cgacgttttt
    11341 caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg caaattccag
    11401 cagcgttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc cggagttacc
    11461 gccgccgata accgctacgc gtttgccttt aaacagcggg ccgtcgcagt gcgggcagta
    11521 ggtcacgcct ttagtgcgat actgatcttc gcccggaacg ttcatgttgc gccattttgc
    11581 acccgtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg tttcaatctg
    11641 gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc tgtcgatcac
    11701 atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccaacttct gcccttcagt
    11761 cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac caccaaaacg
    11821 ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg ccgctgcacc
    11881 cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca gctcttctgc
    11941 cgcacgattt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc
    12001 ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt tgcgatcggt
    12061 gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcaatac
    12121 gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga gcgagtaata
    12181 ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag actgcgcttc
    12241 tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga actcgtggcc
    12301 cagcggggag cctgcaaaac gtggtccctg gttggaacct gggttggtga tcaggaaaga
    12361 cggcttacgc actggcaagc tgttatcttc tttaaaggtg actttgtctg acagttctgc
    12421 gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat
    12481 taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt tcatatttgt
    12541 gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct ctgcggctac
    12601 ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc acccgaagaa
    12661 ttaggtgaat ttccgaagta tttcgcgtca gagcaaggcg actagcctgt gaatccccgg
    12721 gagcttacac aagtaagtga ccggggtgag cgggcgacgc caacgccgct atggcgtgaa
    12781 agacgaagga aatttagatt ttaccaacca ggtccagaga cggagccaga gttgcttcac
    12841 cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac tgagctgctt
    12901 tgattttacg cagcagatca gacgcgtcac ggccaatgcc ttcagcggta acttcgattg
    12961 cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga ccttcatctt
    13021 cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt
    13081 tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg
    13141 tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt
    13201 cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag aagacgctcc
    13261 agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg aatgcctggt
    13321 ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc gtgttttcga
    13381 tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt atcaaatcga
    13441 taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga
    13501 taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc ctttggtatc
    13561 ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg acatggatta
    13621 caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat gacagtgata
    13681 ggttatgcct tttactcgac ttttgcactg actgaaaaga acaaattaat gttaaaaaag
    13741 atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc ttccccagta
    13801 aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc cggaggaatg
    13861 aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac tcccgatggt
    13921 aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag taacacactt
    13981 atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat ggaacaatcc
    14041 cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt ctccgatccg
    14101 ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga ttctggcaaa
    14161 gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc gacagcagcg
    14221 gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc ctctggttgc
    14281 aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt gttaagtgac
    14341 aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta ttacatgagt
    14401 aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct taatatcatt
    14461 atgggaaata aataaaaggt aattcatgga tggttgaact atatccctgc ttttcaacca
    14521 tcctaaaaaa gcatgaattt ctcgctctac cctaagtggc tgtgctattt tagaaaaact
    14581 taatcctgag ttaagcaaaa aaacataatc aataaaaata catacgcaaa aaacattgat
    14641 taagtgaata taccatggaa gaaaaatata aaccggagta gtgtatggcc aatctctacg
    14701 acttgaaaaa gttcgactta aatcttcttg tcatatttga gtgtatttac cagcatttaa
    14761 gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc cagtcgttac
    14821 aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc
    14881 cgacgacaac agggttgaat ctgcatcacc atcttgagaa aaacctcaaa ggccttgaac
    14941 aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc tacggccctc
    15001 agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa gatgcctctg
    15061 tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa ctgttggttc
    15121 accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc gtcatttgca
    15181 tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg cgaattactg
    15241 acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata gccaaatcag
    15301 caggagttga tgatattcaa atggaaatag acgagaagtt tatgaatcgc aaaatttcat
    15361 ttcgtggttc ctctttgctg acaataatta atagcattgc agttacagat ttactgggaa
    15421 tagtccccta tgaactttat aactaccacc gtgactttct taatttaaaa gagataaaac
    15481 tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct tctttaaata
    15541 atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat ataactatag
    15601 acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa aacactctgc
    15661 tttacagtgt ttactttata attaacagtc ttatctcact gttcaccctg taacgttcag
    15721 agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc tggaagctgc
    15781 acgagagcgt attacctaga cactcaacac cctaccccgc gtttgtgtct cattttctgg
    15841 cggcaaagac tctggtttaa tgctgcatct gacagccgaa cttgcccgac aaatgggcaa
    15901 aaaaatctgc gttttgttta tcaactggga ggc
//
LOCUS       DBBBSB010000092        15412 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.092, whole genome shotgun sequence.
ACCESSION   DBBBSB010000092 DBBBSB010000000
VERSION     DBBBSB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.092"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1177)
                     /locus_tag="ACIUS0_002945"
     CDS             complement(<1..1177)
                     /locus_tag="ACIUS0_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0220614.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACIUS0_002946"
     tRNA            complement(1376..1460)
                     /locus_tag="ACIUS0_002946"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACIUS0_002947"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACIUS0_002947"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGB0220615.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACIUS0_002948"
     CDS             2804..4306
                     /locus_tag="ACIUS0_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGB0220616.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACIUS0_002949"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACIUS0_002949"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGB0220617.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACIUS0_002950"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACIUS0_002950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGB0220618.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACIUS0_002951"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACIUS0_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGB0220619.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACIUS0_002952"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACIUS0_002952"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGB0220620.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACIUS0_002953"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACIUS0_002953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGB0220621.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACIUS0_002954"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACIUS0_002954"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGB0220622.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACIUS0_002955"
     CDS             complement(11871..13067)
                     /locus_tag="ACIUS0_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGB0220623.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="ACIUS0_002956"
     CDS             13260..13763
                     /locus_tag="ACIUS0_002956"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0220624.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACIUS0_002957"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="ACIUS0_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGB0220625.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="ACIUS0_002958"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="ACIUS0_002958"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGB0220626.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3968 g         3533 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatctctata aa
//
LOCUS       DBBBSB010000093        15143 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.093, whole genome shotgun sequence.
ACCESSION   DBBBSB010000093 DBBBSB010000000
VERSION     DBBBSB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.093"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACIUS0_002959"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACIUS0_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGB0220627.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACIUS0_002960"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACIUS0_002960"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGB0220628.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACIUS0_002961"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACIUS0_002961"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGB0220629.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACIUS0_002962"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACIUS0_002962"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGB0220630.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACIUS0_002963"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACIUS0_002963"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGB0220631.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACIUS0_002964"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACIUS0_002964"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGB0220632.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACIUS0_002965"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACIUS0_002965"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGB0220633.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACIUS0_002966"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACIUS0_002966"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGB0220634.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACIUS0_002967"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACIUS0_002967"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGB0220635.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACIUS0_002968"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACIUS0_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGB0220636.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACIUS0_002969"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACIUS0_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACIUS0_002970"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACIUS0_002970"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGB0220637.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12258)
                     /locus_tag="ACIUS0_002971"
     CDS             complement(11944..12258)
                     /locus_tag="ACIUS0_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220638.1"
                     /translation="MKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDW
                     TISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIA
                     IK"
     gene            complement(12300..12806)
                     /locus_tag="ACIUS0_002972"
     CDS             complement(12300..12806)
                     /locus_tag="ACIUS0_002972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220639.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12952..13551)
                     /locus_tag="ACIUS0_002973"
     CDS             complement(12952..13551)
                     /locus_tag="ACIUS0_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220640.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13722..14660)
                     /locus_tag="ACIUS0_002974"
                     /pseudo
     CDS             complement(13722..14660)
                     /locus_tag="ACIUS0_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(14723..15097)
                     /locus_tag="ACIUS0_002975"
     CDS             complement(14723..15097)
                     /locus_tag="ACIUS0_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGB0220641.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
BASE COUNT         4191 a         3665 c         3482 g         3805 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt
    12421 aaacttgcaa ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac
    12481 ctgtcacctt gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat
    12541 acccaaagta ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca
    12601 tcactgccaa ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt
    12661 cgagcttgtt ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat
    12721 atatttcctc cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa
    12781 taaatgccac caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt
    12841 ttcgctatca atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca
    12901 caagcaactc caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt
    12961 tctgagcgaa tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat
    13021 atatgcataa ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat
    13081 aatgcctgga taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat
    13141 tacaaaaaaa cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc
    13201 taaagccatt aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac
    13261 aacaattgca tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc
    13321 ccaatcaccg ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa
    13381 taaaataaca aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg
    13441 acgtttaata atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt
    13501 aaattgcccg accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca
    13561 taggcaataa aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat
    13621 gacaatacaa tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca
    13681 tacaaataca acggcaattc aatcatagta aaatactcta tttacattat cagtttatag
    13741 tttttttcag aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc
    13801 aaaataatgc cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag
    13861 gcagtcggaa atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct
    13921 aaaattatag acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt
    13981 tcaagcgagt aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat
    14041 agtttgctca cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc
    14101 atacgtaatt ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga
    14161 catggaaaag atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta
    14221 aaattccctt taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc
    14281 ccttttctgt atgtattttg atctccattc tcttgacgac atcctacaac catatccaat
    14341 tgatttttta tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct
    14401 ccatcaacca ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc
    14461 cccttgcctt tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct
    14521 gtactgtcag tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg
    14581 aaatcattta tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg
    14641 atattaaaag agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat
    14701 tgtgttttta acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta
    14761 aaaccaaact aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat
    14821 caaataaaac gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc
    14881 tagccttggt tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg
    14941 ctgcacatat aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac
    15001 acgcataaaa taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa
    15061 aaaaagaact ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga
    15121 ggttattact aatttggata ttt
//
LOCUS       DBBBSB010000094        15133 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.094, whole genome shotgun sequence.
ACCESSION   DBBBSB010000094 DBBBSB010000000
VERSION     DBBBSB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.094"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACIUS0_002976"
     CDS             complement(86..2365)
                     /gene="maeB"
                     /locus_tag="ACIUS0_002976"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGB0220642.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2654..3604
                     /gene="tal"
                     /locus_tag="ACIUS0_002977"
     CDS             2654..3604
                     /gene="tal"
                     /locus_tag="ACIUS0_002977"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGB0220643.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3624..5627
                     /gene="tkt"
                     /locus_tag="ACIUS0_002978"
     CDS             3624..5627
                     /gene="tkt"
                     /locus_tag="ACIUS0_002978"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGB0220644.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5730..6773)
                     /locus_tag="ACIUS0_002979"
     CDS             complement(5730..6773)
                     /locus_tag="ACIUS0_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGB0220645.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(6899..7474)
                     /gene="nudK"
                     /locus_tag="ACIUS0_002980"
     CDS             complement(6899..7474)
                     /gene="nudK"
                     /locus_tag="ACIUS0_002980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGB0220646.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7542..9521)
                     /gene="aegA"
                     /locus_tag="ACIUS0_002981"
     CDS             complement(7542..9521)
                     /gene="aegA"
                     /locus_tag="ACIUS0_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGB0220647.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9727..11444
                     /gene="narQ"
                     /locus_tag="ACIUS0_002982"
                     /pseudo
     CDS             9727..11444
                     /gene="narQ"
                     /locus_tag="ACIUS0_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11646..14759
                     /gene="acrD"
                     /locus_tag="ACIUS0_002983"
     CDS             11646..14759
                     /gene="acrD"
                     /locus_tag="ACIUS0_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGB0220648.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14858..14917)
                     /gene="ypfM"
                     /locus_tag="ACIUS0_002984"
     CDS             complement(14858..14917)
                     /gene="ypfM"
                     /locus_tag="ACIUS0_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGB0220649.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3563 a         4011 c         3943 g         3616 t
ORIGIN      
        1 aaactcagta ataagctttg ctaatatatt tcaggaaaac cgcttaccct gaatattcag
       61 ggtaagcgtg agagttaaga atgaattaca gcggttgggt ttgcgcttca accacggcca
      121 gcgccaccat attgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
      181 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
      241 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
      301 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt
      361 gcatttcacc atcaatcatc agatctggcg cacgatccct gaccagttcc agcgcctgac
      421 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
      481 cgcgcggctc aataccaaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
      541 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
      601 gcagcagcag cgcattcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
      661 ttttcaccac actaaaatgt tcatgataat cgcccaccgt accgcagatc attgcatcgg
      721 cttccccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcacgct
      781 gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg gtccagtact
      841 ctttaaagcg cggatcggat tcgttattga cgatttcaaa atcaacgccc gctttgatct
      901 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
      961 tcgccagtcc cagcgttacc agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt
     1021 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
     1081 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
     1141 gacgcgtcgc cacgcccgac tccatcgccg cttttgcgac cgcaggagcg atcttaacga
     1201 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgaa
     1261 cgccatacgc tgaagccacc acttcactct gttctgcatg ggccagttct gcaatcgcgc
     1321 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
     1381 ggaagatgaa cgggaagcaa agaacgttgt tcacctggtt cgggtagtcg gaacggccgg
     1441 tacagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
     1501 ggttcgccag cgccaggatc attggtgccc gagccatttt cttcaccatt tcctgggtca
     1561 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
     1621 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccata tttggctcac
     1681 ggccctgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagcccca
     1741 gcgctaccag caggttcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
     1801 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
     1861 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
     1921 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatg ccgccgaagg
     1981 tcggttcgag cgcggcgacg acttcaataa atttgtccgg gtcgagttca tcaacttcaa
     2041 tgtcaaatac atcaatccca gcgaatttct taaatagaac gcccttgcct tccatcaccg
     2101 gtttgcctgc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
     2161 ccgcaaccag gttaccgcgc gcggtatatt tgtaggcttt tagcgggtct ttttcgattt
     2221 caagacaagg tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca
     2281 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
     2341 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
     2401 gtgtgtgggc aacagcccct gcccacaacg tggcgcacat tattaccctg ccggagtcta
     2461 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
     2521 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
     2581 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
     2641 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
     2701 cagctgcgat attgagtcca ttcgccatta tcatccccag gatgccacca ctaatccttc
     2761 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
     2821 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
     2881 ggcggtcaat ttcggtgctg aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt
     2941 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
     3001 cttgtatcag caacaaggcg ttgagaaatc acgcattttg atcaaactgg cttcgacctg
     3061 ggaaggaatt cgcgcggccg aagagctgga aaaagaaggt attaactgca acctgacgct
     3121 gctgttttct tttacccagg cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc
     3181 gtttgtaggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
     3241 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
     3301 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
     3361 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
     3421 tccagtggta cgtaaattaa tcccgccttc tcagacgttc ccacgcccag cacccatgag
     3481 cgaagcggag ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga
     3541 aggcattcgt ctgttcgctg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
     3601 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
     3661 tcagtatgga tgctgtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
     3721 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taatcctacc gacccaacct
     3781 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
     3841 tgctacatct gaccggttac gacctgccgc tggaagaact gaagaacttc cgtcagttgc
     3901 attcgaaaac cccaggccac ccggagattg gctatacgcc aggcgttgaa accaccaccg
     3961 gcccgcttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
     4021 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
     4081 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
     4141 gactaggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
     4201 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
     4261 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
     4321 tgaaagataa gccgtcgctg attatctgcc gtacggtgat tggctttggt tcgccgaata
     4381 aagcaggtaa ggaagaggcg cacggcgcac cactggggga agaagaagtg gcgctggcac
     4441 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagagatt tatcacgcct
     4501 gggatgcccg tgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag tttgccgcct
     4561 ataaaaaggc tcatccgcaa ctggcagaag agtttccccg acggatgagc ggtggtttac
     4621 cgaaggactg ggagaaaacg actcagaaat atatcaatga gttacaggca aatccggcga
     4681 aaatcgctac ccgtaaggct tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg
     4741 agttgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
     4801 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
     4861 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
     4921 tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
     4981 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
     5041 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
     5101 gcgatcagat ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
     5161 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
     5221 aagagattgc tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
     5281 tgattgccac cggttcagag atggaaatta ccctgcaagc ggcagagaaa ttagcaggag
     5341 aaggtcgcaa tgtacgcgta gtttccctgc cctcgaccga tattttcgac gcccaggatg
     5401 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
     5461 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
     5521 gttacgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
     5581 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg
     5641 atgctgtatg ttcccctcac cctaaccctc tccccagaag ggcgagggga cggtccgtgc
     5701 gctttttagg gccgcatctg cgattttgtt taacgggtga tccacaacgt gggcctggcg
     5761 tctggcccat gccagttatc gcaggtaggt tctgcggcgt aacgcaccag gcgaaaacgc
     5821 tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct
     5881 aaggtcacca gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc
     5941 gtctcctggc cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc tagcggcttt
     6001 ttacgcgaca caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc
     6061 atcatcagtg ctttatcatc ggtcagcgca gtgacattca cctcacgacg taacggatca
     6121 agcgagcagc gcagaccatt catccgccag tttccataat ccagcaaatc gttacgttct
     6181 tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg
     6241 ggcggtacgc tgagaggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg
     6301 cgcttttgct gagcatcaat aaacaacaac gccgctttta agccgctcag agaaatggtc
     6361 tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa ttatctgcaa aaacgcggtg
     6421 atggttgccg tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt
     6481 aatgccaacg gctcaccatc taatagcagc cgtggcgcta tctccccttc agacgcatcc
     6541 ggcgacttca acccaccgcg ctcaatacgt aaaacggcat cggtatgcgc cccagcgctg
     6601 cggctcaggg tcatcaccag tccattatga tcgcccgtat tgcgcgccac acaaaaattt
     6661 tgattattac aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc
     6721 aaagacgtcg gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt
     6781 acgaccccag aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg
     6841 ctcaatcgga tttgtcggat tgagccaata ataaacaacg aaatattatt gctatttttc
     6901 agtccattaa atgtgacatt tgcaaatagt taagcaataa caccgtctta ccgtcacgta
     6961 tctcgccggt tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat
     7021 cttcatcttc aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa
     7081 aaaagtggat tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca
     7141 cttcgcccac ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt
     7201 cgtcgttatc cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa
     7261 cccaggtagc gacacggaac tgacgaatca gaaccacgct ctttttcttc gcgttgtaca
     7321 ggaggatcgt cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc
     7381 catctttgcg cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa
     7441 ttttgtcttt aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca
     7501 attatattac gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat
     7561 tttaccccca gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta
     7621 accaccagat ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc
     7681 tggtaacggt actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca
     7741 ccgtgcgact ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca
     7801 tcggctggca tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca
     7861 tccggctcgc caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc ttgctcattc
     7921 agctcaagcg ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc
     7981 ttcacctctt tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg
     8041 ttactcgcgc cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc
     8101 agtaccacga cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc
     8161 cccatcacct gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca
     8221 tcttcattgg gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg
     8281 tcgtattgtt ccaaaagcaa atccaaagag acatctttac ccacttcaca attgagttcg
     8341 aagtgaatcc ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt
     8401 ttgaaagaag gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc
     8461 gtcaccgcca cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgcc
     8521 ccgataatcg ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct
     8581 ttcgccaacg cctgatctga aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc
     8641 tcatcgcgaa tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta
     8701 atttccggca gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct
     8761 ttcaccagtt caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg
     8821 ctgtgctcgc cgaacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat
     8881 ggcagataaa tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg
     8941 ccacgcgcgg gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgtgca
     9001 gcggtactgg catgccacgg ttgatgctcc tgacgtgcgg tgcgcaagcg gcgggatttc
     9061 gccatgccgg agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctcg
     9121 acacaggcag gaccgttttc gcgccctgca caaaggtcgc atttatgcgc cgtggctttt
     9181 acttttcctg ccgcgacggg tgtcaggacg atttgcatcg taccaaacgg acaggccacc
     9241 acgcaggatt tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg
     9301 ctgattgcgc cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc
     9361 actgcactac gttgctgctg atgtttgata accgtaattc ggggatgaaa atggtgttgg
     9421 ctcaggacat gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa
     9481 cccagacatt gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt
     9541 tgtaaaaacc ttattcttta tatgagtgtt gttattaccc gacttacagg ggaaccggca
     9601 atgttcatgt gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc
     9661 ccatcactga ctaaattgcg tttcagcgaa ctggaacatt aatgattttt tgtggagaag
     9721 acgcgtgtga ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt
     9781 gtgctgctgt cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc
     9841 gacgctgagg ctatcaatat tgccggatcg ctgcgtatgc agagttaccg cctgggctac
     9901 gacttgcaaa gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg
     9961 cattcaccgg tattaaccaa cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc
    10021 tatgcgcatc tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg
    10081 ccgtggtatc aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct
    10141 ttacagcact acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt
    10201 atcggtattt tcacgctggt cttttttact ttgcgccgca tacgccatca ggtggttgcc
    10261 ccgctgaatc agctggttac cgccagtcag cgtattgaac acgggcagtt cgactcgccg
    10321 ccgctggata ccagtctgcc gaatgagctt ggtctgcttg caaaaacctt taaccaaatg
    10381 tcgagcgagc tgcataaatt gtacctttcg ctggaggcgt cagtagaaga aaagacccgc
    10441 gatctccacg agaccaagcg tcgtctggag gtgttgtatc agtgcagtgt tcccaggcgc
    10501 tgaacaccag ccagattgat gtgcattgtt tccgccatat tttgcagatt gttcgcgaca
    10561 atgaagcagc tgaatatctg gagctgaacg ttggggataa ctggcggatt agcgaaggga
    10621 agccaaaccc tgagctgcca atgcagattt taccggtgac aatgcaagag acggtttacg
    10681 gcgaactgca ctggcaaaat agtcacgttt catcatcaga accgctgctt aacagtgttt
    10741 catcgatgct gggacgcggt ttgtacttta atcaggcgca gaagcatttt cagcagttat
    10801 tgttgatgga agaacgtgcg accatcgccc gcgaattgca cgactcgctg gctcaggtgc
    10861 tttcttactt acgtatccag ttgacgttac tgaagcgttc gataccggaa gataacgcca
    10921 ccgcacaaag tatcatggcc gatttttccc aggcgttgaa tgatgcttat cggcagttac
    10981 gcgagctgtt gactactttc cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga
    11041 gggaaatgct ggatacgtta caaaatcaaa ccagcgctaa actgaccctc gactgccgtc
    11101 tgccaaccct ggcactggat gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag
    11161 cggtgctgaa tgcgatgaag cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg
    11221 cgccggacgg caatcacacg gtttatatcc gtgataacgg gattggtatc ggtgaaccga
    11281 aagaacccga aggtcattat ggactgaata tcatgcgcga acgcgcggaa cggctgggcg
    11341 ggacgctgac tttttcacaa ccttccggcg gcggcacgtt agtgagtatt agctttcgct
    11401 ccgcagaggg tgaggggaag gggaacacgc cggctgaggt gtaaataatt accctcacac
    11461 cggccctctc cctttaaagg cgagagggaa aaacgtgccc atattgacct gtaagcgagt
    11521 gtgtcagcaa tctcatgata atttacatta actccttttt ttctccacga ttggctcgta
    11581 ccttgccgct acagtgaagc aagtcaagcc tacaacaata cgcagaaaca cgaggtcctc
    11641 ttttaatggc gaatttcttt attgatcgcc ccatttttgc ctgggtgctg acaatcctgt
    11701 tgtgtctgac aggtaccctg gcgatttttt cattgcccgt tgaacaatac cccgatctcg
    11761 cgccaccgaa tgtgcgagtg accgctaact atcccggcgc atcggcccag acgctggaaa
    11821 acaccgtgac ccaggttatc gagcaaaata tgaccggcct cgataatctg atgtatatgt
    11881 catctcagag cagtggcacc ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg
    11941 atccggacga agccgtgcag caagtacaaa accagctgca atcagccatg cgaaagttac
    12001 cgcaggcggt gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc aacattctga
    12061 ccattgcctt cgtctctacc gatggctcga tggataaaca ggatattgct gattatgttg
    12121 ccagtaatat tcaggacccg ttaagccgcg tgaatggcgt cggggatatc gatgcctatg
    12181 gttcgcaata ttccatgcgt atctggctgg acccggcgaa actcaacagt ttccagatga
    12241 cggctaaaga tgtcactgat gccattgagt cacagaacgc gcagattgcg gttgggcaac
    12301 ttggtggtac accttccgtc gataagcagg cgctcaacgc caccattaac gcccagtcac
    12361 tgctgcaaac accagaacag ttccgcgata tcaccttgcg ggtgaatcag gacggctcag
    12421 aggtaaggct gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac gattatctta
    12481 gccgcttcaa tggtaagcca gcctccgggt tgggggtaaa actggcctcc agcgcaaacg
    12541 aaatggcgac agcggagctg gtgctcaatc gtctcgacga gctggcgcag tatttcccgc
    12601 acggactgga atacaaggtg gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag
    12661 acgtggtgaa aacgctgctg gaagctatcg ctctggtttt cctcgttatg tatctgttcc
    12721 tgcaaaactt ccgcgccacg ctgataccca ctatcgccgt gccggtggtg ttgatgggaa
    12781 ccttctccgt actttacgcc ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg
    12841 tgctggcgat cggtctgctg gtggatgacg ccatcgtggt ggtggaaaac gtcgaacgta
    12901 ttatgagtga ggaaggactc actcctcgcg aagccacacg taaatcgatg gggcagatcc
    12961 agggggcact ggtcgggatt gcgatggtgc tttcggcggt atttgtacca atggccttct
    13021 tcggcggcac caccggtgcc atctatcgcc agttctctat taccattgtt gcggcgatgg
    13081 tgctgtcagt actggtagcg atgctcctca ctccggctct gtgtgccacg ctacttaagc
    13141 cactgaaaaa aggtgagcat cacgggcaaa aaggtttttt tgcctggttt aaccagatgt
    13201 ttaaccgcaa cgccgaacgc tacgaaaaag gggtggcgaa aattctccac cgtagcctgc
    13261 gctggattgt gatttatgtc ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc
    13321 cgacgtcgtt cttaccgctg gaagaccggg gcatgtttac tacctcggta caattgccca
    13381 gcggttcaac ccaacaacag accctgaaag tcgttgagca aatcgagaaa tattacttca
    13441 cccatgaaaa agacaacatc atgtcggtgt ttgccaccgt tggttctggc cctgggggta
    13501 acgggcaaaa cgtggcgaga atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta
    13561 agaccggcac ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac caaattaaag
    13621 aagctcgcgt tatcgccagc agcccaccag caattagcgg tcttggtagt tctgcaggtt
    13681 ttgatatgga gttgcaggac cacgctggag cgggtcacga tgcgctgatg gcagcacgta
    13741 atcagttgct ggcgctggcg gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc
    13801 tcgatgacag tccgcagttg cagattgata tcgaccagcg taaagctcag gcgctgggcg
    13861 ttgatatcga cgatattaac gacacactgc aaaccgcctg gggttcgagc tatgtgaatg
    13921 actttatgga tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca
    13981 tgctgccaga tgacatcaat ctctggtatg tccgaaataa agatggcggc atggtgccct
    14041 tctctgcttt cgcgacctca cgctgggaaa caggctcgcc gcgtctggaa cgctataacg
    14101 gttattctgc ggttgagatt gttggggaag ccgcaccggg ggtcagtacc ggtacggcga
    14161 tggatattat ggaatcgtta gtgaagcagc tgccaaacgg ctttggtctg gagtggacgg
    14221 cgatgtcgta tcaggagcgg ctttccggcg cgcaggctcc ggcgctgtac gccatttcct
    14281 tgctggtggt attcctgtgt ctggcggcat tgtatgaaag ctggtcggta ccgttctcgg
    14341 taatgctggt cgtgccgctg ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc
    14401 tggaaaacga cgtttacttc caggtgggcc tgttaacggt cattggttta tcggcgaaaa
    14461 acgccatcct gatcgtcgag tttgctaacg agatgaacca aaaaggccat gacctgtttg
    14521 aagcgacgct ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat
    14581 ttatcttcgg cgtattgcca atggcaacca gcacgggtgc cggttccggt ggtcagcatg
    14641 cggtgggtac tggcgtaatg ggcgggatga tttcagctac tattctggct atttacttcg
    14701 tgccgctgtt ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag
    14761 caataaaaaa ggcgacatgc caatgtgtcg cctttttcaa ctttccgatt aagaacctgc
    14821 tcagcgggtt cttgctgttt atactttgtc tcaggaatta cttacgaagc ataacttcga
    14881 taaagtcttt ccagttcccc agttcacgtt caatcataac aacctctctt ataattatgg
    14941 gtattctacg gaaacaatat accgtggtga agctaattta ctcgattgct gcgatgacta
    15001 cctccggggg acaaacctta tgtaaatact atggtcctgc ggtgataatt tgtatgtgat
    15061 acacagcaac atttcgagat attcatacgg catctaatac tgatttaatt ctggttaaaa
    15121 tacagacaga taa
//
LOCUS       DBBBSB010000095        14725 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.095, whole genome shotgun sequence.
ACCESSION   DBBBSB010000095 DBBBSB010000000
VERSION     DBBBSB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.095"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(267..1208)
                     /gene="fabY"
                     /locus_tag="ACIUS0_002985"
                     /gene_synonym="yiiD"
     CDS             complement(267..1208)
                     /gene="fabY"
                     /locus_tag="ACIUS0_002985"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGB0220650.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1253..1690)
                     /gene="dtd"
                     /locus_tag="ACIUS0_002986"
     CDS             complement(1253..1690)
                     /gene="dtd"
                     /locus_tag="ACIUS0_002986"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGB0220651.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1687..2559)
                     /locus_tag="ACIUS0_002987"
     CDS             complement(1687..2559)
                     /locus_tag="ACIUS0_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGB0220652.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2553..3152)
                     /gene="yihX"
                     /locus_tag="ACIUS0_002988"
     CDS             complement(2553..3152)
                     /gene="yihX"
                     /locus_tag="ACIUS0_002988"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGB0220653.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3251..4036)
                     /locus_tag="ACIUS0_002989"
     CDS             complement(3251..4036)
                     /locus_tag="ACIUS0_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGB0220654.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4070..4966)
                     /gene="yihV"
                     /locus_tag="ACIUS0_002990"
     CDS             complement(4070..4966)
                     /gene="yihV"
                     /locus_tag="ACIUS0_002990"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGB0220655.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5134..6030
                     /gene="yihU"
                     /locus_tag="ACIUS0_002991"
                     /pseudo
     CDS             5134..6030
                     /gene="yihU"
                     /locus_tag="ACIUS0_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
     gene            6054..6932
                     /gene="yihT"
                     /locus_tag="ACIUS0_002992"
     CDS             6054..6932
                     /gene="yihT"
                     /locus_tag="ACIUS0_002992"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGB0220656.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6949..8190
                     /gene="yihS"
                     /locus_tag="ACIUS0_002993"
     CDS             6949..8190
                     /gene="yihS"
                     /locus_tag="ACIUS0_002993"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HGB0220657.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8328..9230
                     /locus_tag="ACIUS0_002994"
     CDS             8328..9230
                     /locus_tag="ACIUS0_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGB0220658.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9429..11465
                     /gene="yihQ"
                     /locus_tag="ACIUS0_002995"
     CDS             9429..11465
                     /gene="yihQ"
                     /locus_tag="ACIUS0_002995"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGB0220659.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11511..12885
                     /locus_tag="ACIUS0_002996"
                     /pseudo
     CDS             11511..12885
                     /locus_tag="ACIUS0_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12928..14331
                     /locus_tag="ACIUS0_002997"
     CDS             12928..14331
                     /locus_tag="ACIUS0_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220660.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14399..>14725
                     /locus_tag="ACIUS0_002998"
     CDS             14399..>14725
                     /locus_tag="ACIUS0_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGB0220661.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3586 a         3815 c         3700 g         3624 t
ORIGIN      
        1 aaaagtatgg caccagaggg ggttttgact atcaaaaatc gctcaaacct gaactaatat
       61 gatgagttaa gcattatctg caataattgc cactcagctc tacaaataat atttaaatgc
      121 tcctgaacag tgatgtagca agacacgtat ggtgccaacg aagaataaaa atttataaag
      181 ttcttattca atataaccgg aatagtcagg gactgatgta aatgaccgga atgaatgaga
      241 gaggcagcac cgcccctctc cataagctac tcttcttcgt tcccgccctc ttcatacggg
      301 ccaaatggct tcgcgggcag aacgatatac gtgccttcaa acactgcacc cggcgtctcg
      361 tcgccaaaga tttcgacctg catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg
      421 agatcgccgc ttaaggcacc aaggtcggct accgcatggg gtttaccgct aatcggtttg
      481 ctgtagcgga tatgcgcatc cgccagaata atcgttccgc cgaggtggcg ttcacgcagc
      541 atcagccaga taagccccca accggtgagc gtcgccagcg agaataaact cccggcaaac
      601 agcgtatggt gcggattctg attgccggtt tccggcatgg tggtgataaa tttttgcccg
      661 gtatattgtt gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc
      721 tgttgcagct gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcgacgggc
      781 ttaatcatca aaaaatggcg aatcggcgtg gtggttggcg tggtgatttc tccctgatta
      841 acaaacccca gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc
      901 ttaacgcctt cctgacgcgc caccgactcc agggtcatcg ccatcagcgt tcctaaccct
      961 ttgtcctgca cgtccgggtg aacgaccata aagcgaatgg acgcttcatt gtcggcatta
     1021 atatacaatc ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc
     1081 gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac
     1141 atttcccagc gaaagtgata gtaacgctct aactcttctt ctgtttgtgg aacctgaagg
     1201 tgatacatag cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct
     1261 gcaaccagaa tgtcacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc
     1321 gtcctgtttg cgtgttcatc tcttgctggc ggcagcgttc gacgaaatag tcatataacg
     1381 cctctgcgcg atccggtgat gcgcctttgg agaaacttgg gcgcatcccc cgttcggtat
     1441 ctgcggcgag ggtaaactgg gaaaccacca gtacactgcc gcccgcctgt tgcacgttga
     1501 gattcatctt gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac
     1561 ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc
     1621 caatttcgcc cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta
     1681 atgcaatcat ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc
     1741 cccgagagtg acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca
     1801 aacaaagaga atggggatca ccgccagcac accgtaaatg agctgatatg acgggaacat
     1861 ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac aggagtgcgg cgacaaacgc
     1921 gccgacaatc gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca
     1981 gaaggagatc cacgacaaca gcaacggaaa aatacgcagc acgttgtcga tgacagtatt
     2041 gagatcgctc gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc
     2101 cagcagcggc cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg
     2161 cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcaacaataa
     2221 cgcggtgacg atcagcccgc acgccccaac ggcggtcatc ttgttggaat tagcaacaaa
     2281 ttgttcgata taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa
     2341 gtgacgtaac tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac
     2401 aacggcaacc agcggcacta atgagagcaa cgacacatag gcaaggttac ctgccagggt
     2461 tgtcatgttg tcctcatcaa tacgttgcca gagtagtttt agccaggccc atagtggacg
     2521 ggtacggtgc ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc
     2581 gggatggtgg ttttatcttt caccagaata ctggtaatgc ctagctgatt agctccttct
     2641 atattatcgg cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc
     2701 aaaacatgct ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag
     2761 atatggtcag cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg
     2821 cggttggtat tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg
     2881 gcgatcactt ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg
     2941 tagcttagcg gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt
     3001 tccccacgct catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc
     3061 agcggaatac gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca
     3121 atcacattac ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat
     3181 taactgtagc gggaaaggat gggtttggct atgtggcaag gtatgaagcc aggttaacgg
     3241 tcacaaaaat ctacgcgtct tcctggggaa gaatgatttc aatttggctg tcttgtaagc
     3301 gtgattttag cgcagcgggc ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt
     3361 taccaatttc aaccgccgcc gaagcatgat acttagtgtg atcggcgacc agcagaatat
     3421 ttctcgcgtg cgccatcatc gttttcacca cgttagcttc gtttacatca aactccatca
     3481 acgcgccatc gctctcaatc gccccaacgc ttgttaccag ataatcagca cgaaaatcag
     3541 ccacaaagga cgccgctgaa gggccaatga tcccgctatt atgagagcgc aacgtaccgc
     3601 cgggcaccat cacttcaaag cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt
     3661 tggtgattat ccgcaaatga ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg
     3721 taccaatggt gataaatatt gttgaaccat caggaatata gtctgccacc gcttcggcaa
     3781 tcgctttttt tttctcggtt tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc
     3841 tggaagcccg acccgcgcca ccgtgatggc gcgtaatcag gccttgctcg cttaatttac
     3901 gaatatcccg gcggaccgtc tgcgtggaga catccagcag atttgccagc tcatcaatat
     3961 tcatataccc acgctcggcg atcagcatga ggagttggtc gtgccgcggg ttaccggtta
     4021 gttcggtaag gctcatgaaa attcctcgaa aaaccatcac cctggcattt tatacaaaaa
     4081 gtgacaaaaa agatcgggtt tgatcacagt cagggatccc ggctcgtcca ccgggacgtg
     4141 tgcattttaa cgccgctaca ccgctggcga agcggactga ctccgctaaa tccccacttg
     4201 ttgccagcgc caccgccaaa gcgccgtgaa aaacatctcc cgcacctgtg gtatctacca
     4261 catcaacttt gaaggccggt tgatgctgac gcccaccatt ttccagccag tcgcagcctg
     4321 cgctaccctg ggtcacatag acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac
     4381 tggccatctc tttcacgccc gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat
     4441 cgcttaatgc caccagctca ctgatatcct gcggcgtaat gtccccgtcc agaacggtca
     4501 tcacacccgc ctgacgggcc agggtgaagg cttttttagc gccgtcgtgc cagcgtacat
     4561 ctgccagcac aacatcccac tgagagaaat caatttcctc caaccactct gcgtcaggca
     4621 gcagatccgg gctggggtag ttaatgatta tccgctcgcc tttggtatcc accatgatgg
     4681 cggattgcga agatttcgcc tggttatacc gtttggtgta acgggtgtta acgccccagg
     4741 attccagctc tgccagcagg ctgttgcccg cgtcgtcatc acctacgcga ccaataaaat
     4801 cgacctgcgc ccccagcctt gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa
     4861 cttccgtata atttctcgcc acgtatttac cgctctccgt cggtaaccct tccacgtaat
     4921 agatgcgatc catcacggtt atacctacac aagcaacacg aatcatggtc attccttaaa
     4981 cattttggga tgcgttcatt ttaatttcac caattgttta aaaagtgacg gctgtcaatt
     5041 ttttgacgat aaattacaaa ttcgatcaaa aacagacaag caaagcatgt cataaatgac
     5101 aaaaagtgac atcactgtat tcaggagagg gttatggcag caatcgcgtt tatcggttta
     5161 ggacaaatgg gttcgccaat ggcgagcaat ttattgcagc aagggcatca acttcgcgtc
     5221 tttgatgtga acgccgaagc tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc
     5281 aacccggcgc aggccgctaa agatgccgaa tttatcatta ccatgttgcc gaatggcgat
     5341 ctggtgcgca acgtgttgtt cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg
     5401 ctggtcattg atatgtccac catccatccg ctgcaaaccg ataaattgat tgccgatatg
     5461 caagccaaag gcttcagcat gatggatgtt ccggtaggcc gtacttctgc aaatgccatt
     5521 accggtactc tgttactgct ggctggcggc accgctgaac aagttgaacg tgccacaccg
     5581 atcctgatgg cgatgggcag tgagttgatc aacgctggcg gtccgggcat ggggatccgc
     5641 gttaagctca tcaacaacta catgagcatc gcgctcaatg cgctttcggc agaagccgcc
     5701 gttttgtgcg aagccctgaa tcttcccttc gatgttgccg tcaaagtgat gagcggtacc
     5761 gccgccggta aaggtcactt caccacttcc tggccaaaca aagtcctcag cggagatctt
     5821 tctcccgcct tcatgatcga tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc
     5881 aaccagctgc atgtgccaat gccgctgggg gccgcctcac gggaggttta tagctaggcg
     5941 cgcgcagcgg gtcgcggtcg ccaggactgg tccgccattc tggaacaggt ccgtgtcagt
     6001 gccgggatga ctgccaaagt aaaaatgtaa cgactggata aaggaataaa tgaatgaata
     6061 agtacaccat caacgacatt acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc
     6121 agcgcgaagc catgcgcatg atgtttgccg cggctggggc acccgctccg gtagccgata
     6181 gcgttttaac tgatttcaaa gttaacgctg caaaggccct ctcgccttat gcctcggcga
     6241 ttctggtaga tcaacaattc tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa
     6301 gttgcgccat gattgtcgcc gccgatgagt tcattcctgg caacggtatt ccggtcgata
     6361 gcgtggttat tgaccgcaaa atcaatccgc tacagatcaa acaagacggt ggcaaagcct
     6421 taaaactgct ggtgctgtgg cgtagtgatg aagatgcgca gcaacgtctg gatatggtga
     6481 aagagttcaa cgaactgtgc cactcacacg gtctggtaag catcattgag ccagtcgtcc
     6541 gcccaccgcg tcgtggcgat aaattcgatc gcgaacaagc gatcatcgat gccgccaaag
     6601 agctgggcga cagtggcgct gacctctaca aagttgaaat gccgctttat ggcaaaggtc
     6661 cgcaacaaga gcttctctgt gcttcacaac gtctgaatga ccatatcaat atgccatggg
     6721 tgatcctctc ttccagtgtc gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga
     6781 cggcgggcgc atcgggattc ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac
     6841 cagacaacga gctgatgctc cgtgacgttt gcgcaccgaa attacaacaa cttggcgata
     6901 tcgtcgacga aatgatggct aaacgccgct aagatgagga taaaaggaat gaaatggttt
     6961 aacaccctaa gccacaaccg ttggctggaa caggaaaccg accgcatctt tgattttggt
     7021 aaaaattccg tagtgccgac tggttttggc tggttaggca ataaagggca aatcaaagaa
     7081 gagatgggca cccatctgtg gatcacagct cgtatgttgc acgtttattc cgttgctgcg
     7141 gcgatgggtc gacctggcgc ttacgcgttg gttgatcacg gtattaaagc catgaacggc
     7201 gcactgcgtg ataaaaaata tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg
     7261 gatgcctcca aacagggcta tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc
     7321 acaacgggtc acccggaagc gcgcaagctg ctcgattaca ccattgagat tatcgagaaa
     7381 tatttctgga gcgaagaaga gcagatgtgc ctggagtcct gggacgaagc cttcagcaaa
     7441 accgaagagt accgcggcgg caatgccaat atgcacgcgg tggaagcttt cttgattgtt
     7501 tatgacgtca ctcacgacaa aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt
     7561 atccacgacg tcgccagaaa taatcattat cgcgttaacg aacacttcga tacccagtgg
     7621 aatccgctgc cggattacaa caaagataac ccggcgcatc gcttccgcgc gttcggaggt
     7681 acaccaggcc actggatcga atggggccgt ttaatgctgc acatccatgc tgccctggaa
     7741 gcccgttgcg aacaaccacc agcatggctg ctagaagatg ccaaaggtct gtttaacgcc
     7801 accgtgcgcg atgcctgggc acccgatgga gcggacggga ttgtttatac cgttgactgg
     7861 gaaggaaaac cggtggtccg cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg
     7921 gtctacgcgc tctacaccgt cactggcgat cgccagtatg aaacctggta tcaaacatgg
     7981 tgggagtact gcattaagta cctgatggac tatgaaaatg gttcctggtg gcaggagctg
     8041 gatgcggaca ataaggtcat caccaaagtc tgggacggca aacaggatat ttatcacctg
     8101 ctgcattgcc tggtgatccc gcgtatcccg ttagcgcccg gcatggctcc agcggttgca
     8161 gcgggtctgc tggatattaa tgcgaaataa ctgatctgaa aatagggtgc gggttttccc
     8221 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg
     8281 acatcagcct cacgggaggc tgtatgtcgt taattaaggt gccttgtatg caaatcacca
     8341 atatgcattg cagtggacag accgtttcac ttgccgcggg ggattatcac gcgaccattg
     8401 tgaccgtcgg tgctggtctg gctgaattaa cctttcaggg ttgtcattta gtcatcccgc
     8461 acaaacccga agagatgccg ctggcgcatc tgggtaaagt gcttattccg tggccgaacc
     8521 ggatcgccaa cggctgttac cgttatcagg ggcaggaata tcagttaccc attaatgaac
     8581 atagttcaaa agccgctatt cacgggctgc ttgcctggcg agactggcaa attagtgaat
     8641 taaccaccac cagcgttacc ctgacagctt ttctgccgcc cagttacggc tatccgttta
     8701 tgctggcttc acaagttgtt tattcgctca acgcacacac gggtctttcc gttgagatcg
     8761 caagccagaa tatcggcact gtcgcagcgc cttatggcgt gggtattcat ccttatttaa
     8821 cctgcaactt aacatctgtt gatgaatatc tgttccagct tccggcaaac caggtttatg
     8881 ccgtcgatga acacgtaaat ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt
     8941 tcacgcaggc gaaaaaaata gccgcgacaa aaatcgacca cacctttaaa accgctaatg
     9001 atttgtggga aatgaccatt acccatccac agcaggcgct ttcggtaagc ctttgttccg
     9061 atcaaccctg ggtgcaggtt tatagcggtg aaaaattaca gcgtcaggga ctggcagttg
     9121 aacctatgag ttgcccgcct aacgccttta attcaggtat cgacttatta ttactggaac
     9181 caggaaaacc gcaccgcctg ttttttaata tttacggaca gagaaaataa cttgtattga
     9241 ttagcagtca aaatcattga agaacatttc actcgctaaa ggcaacatta tgtatcttca
     9301 atttattaag ttaaaaacag ctcagcttct gtaaagtttt tatatatcag ttttatgaga
     9361 atcattttac ataagcctgt aataacagga aggcaacaat ctcgttctca actctatgga
     9421 gattagttat ggatacgcca cgtccacagt tattagattt tcaatttcat cagaataacg
     9481 acagttttac cctacatttt caacaacgtc ttattttaac ccataacaaa gataatcctt
     9541 gtttatggat tggctcaggt atagcggata tcgatatgtt ccgcggtaat ttcagcatta
     9601 aagataaact acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg
     9661 gttggttaat tcatttcagc cgtggttctg acattagcgc cacgctgaat atctctgccg
     9721 acgatcaggg ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgtatct
     9781 ggctgcgcct tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct
     9841 acttcgatct gcgcggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca
     9901 acaaacaaac ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc ggcgactatt
     9961 actggacttt cttcccacag cctacgtttg tcagcacgca gaagtattac tgccatgttg
    10021 ataacagttg ctacatgaac ttcgacttta gcgccccgga ataccatgaa ctggcgctgt
    10081 gggaagacaa agctacgcta cgttttgaat gtgctgacac atacatctcc ctgctggaaa
    10141 aattaactgc cctgctggga cgccagccag aactgcccga ctggatttat gacggggtaa
    10201 cgctcggcat tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg
    10261 cgggcgtgaa ggttaacggc atctgggcgc aggactggtc tgggatccgt ataacctctt
    10321 ttggcaagcg cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt
    10381 cacgcattaa gcagtggtat caggagggcg tgcagttcct ggcctatatc aacccgtatg
    10441 ttgccagcga taaagatctc tgcgaagaag cggcacaaca cggctatcta gcaaaagatg
    10501 cctctggcgg tgactatctg gtggagtttg gcgagtttta cggcggcgtt gtcgatctca
    10561 ctaatccaga agcctacgcc tggttcaagg aagtgatcaa aaagaacatg attgaactcg
    10621 gctgcggcgg ctggatggct gacttcggcg agtatctgcc caccgacacg tacctgcata
    10681 acggcgtcag tgccgaaatt atgcataacg cctggcctgc gctgtgggcg aagtgtaact
    10741 acgaagccct tgaagaaacg ggcaagctcg gcgagatcct cttctttatg cgcgccggtt
    10801 ctaccggtag ccagaaatac tccaccatga tgtgggcggg cgaccagaac gtggactgga
    10861 gtctcgacga tggcctggcg tccgtagtcc cggcggcgct gtcgctggca atgaccggac
    10921 atggtctgca tcacagcgac attggcggtt acaccaccct gtttgagatg aagcgcagca
    10981 aagagctgct gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg
    11041 aaggtaaccg tcctggcgac aactggcagt ttgacggcga tgcggaaact atcgcccatt
    11101 ttgcccgtat gactaccgtt ttcaccaccc tgaaacctta tctgaaagag gccgtcgcgc
    11161 tgaatgcgaa gtccggtctg ccggtcatgc gcccgctgtt cctgcattac gaagacgatg
    11221 cacataccta ttccctgaaa tatcagtacc tgttaggccg cgacattctg gtcgctccgg
    11281 tacacgaaga aggtcgcagc gactggacgc tctatctgcc ggaggataac tgggtacatg
    11341 cctggacggg tgaagcgttc cggggcgggg aagttaccgt taatgcgccc atcggcaagc
    11401 cgccggtctt ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg ttaaacagca
    11461 tctaatcaca tccgcccgtg gggaaactca tgggcaaagg gagaacatcg atgagtcaca
    11521 tcacaacgga agatccagca actttacgcc tgccctttaa agagaaactc tcttacggta
    11581 ttggcgacct ggcctctaac atcctgctgg atatcggtac gctttatctt ttgaagtttt
    11641 ataccgacgt tctggggctg ccaggcacct atggcggcat tatctttttg atttcaaaat
    11701 tctttactgc gtttaccgat atgggtaccg gcattatgtt ggattcccga cgcaagatcg
    11761 gtccaaaagg taagttccgt ccttttattc tgtatgcgtc attcccggtc accttactgg
    11821 cgatcgccaa ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg atggccacta
    11881 ttctgtttat gctttacgga ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg
    11941 tcccggcaat caccaaaaac cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg
    12001 gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt gccagttatg aatcttatcg
    12061 aaggtaatca gaaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc
    12121 tgtttatgtg gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca
    12181 atccggcgca aaagcctggc ctgttgcaat ctttccgcgc aattgccggt aaccgcccac
    12241 tgttcattct gtgtattgcc aacctctgca ccttaggggc gtttaacgtc aagctcgcca
    12301 ttcaggtcta ttacacccag tacgtactta acgatcccat cctgttgtcg tatatgggat
    12361 ttttcagcat gggctgtatt ttcatcggcg tgttcctgat gcctggcgca gtcagacgtt
    12421 ttggtaagaa gaaggtctat atcggcggcc tgctgatttg ggtgctgggc gatctgctca
    12481 actatttctt cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg
    12541 gctcagcgcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga gtggcgcacc
    12601 ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa agtgtctcag
    12661 gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt gccgaacgtc
    12721 gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta cccaagcgca
    12781 ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga gaagatgtat
    12841 gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata ataaacgacg
    12901 ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac attaaaactg
    12961 aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt aatgctctgc
    13021 atcggtactc tgtatctcct caaactttat accgatgaat tagggatgcc tgcttactat
    13081 ggcgggatta tctttctggt cgcgaagttt tttaccgcgt ttaccgatat gctcaccgga
    13141 tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc ctttatttta
    13201 tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac caccttttgt
    13261 ttgccggtta aaacgacgat tgccaccacg ctgtttatga tgtttggact ttcatacagt
    13321 ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc gaatgagcgc
    13381 gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat ttgtaccgtg
    13441 gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc ctgtgcggca
    13501 cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg cgtcaaagag
    13561 cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc tttttgcgcg
    13621 atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac tctggcggca
    13681 tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa tgatattaat
    13741 ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt attactggtg
    13801 ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat ggtgctatgg
    13861 gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt cgtcatgttc
    13921 tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc actggtacca
    13981 gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc tgtttatacc
    14041 ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc aggcattatg
    14101 ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt acagggtttg
    14161 cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt gacgatggga
    14221 ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat caaccaacgc
    14281 aaaaataaag agatggcgac agaaaaaaaa acggcttccg taacgttata aatgtcaata
    14341 gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg gacttattat
    14401 gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg tatttgctgg
    14461 cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg aggttatgct
    14521 acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata cctacacctt
    14581 tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt atcctttatt
    14641 caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata agagtatcag
    14701 ttcgggtggt gcagtgtatc tggac
//
LOCUS       DBBBSB010000096        14644 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.096, whole genome shotgun sequence.
ACCESSION   DBBBSB010000096 DBBBSB010000000
VERSION     DBBBSB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14644
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.096"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACIUS0_002999"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="ACIUS0_002999"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGB0220662.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACIUS0_003000"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="ACIUS0_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGB0220663.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACIUS0_003001"
     CDS             complement(2297..2440)
                     /gene="yqgG"
                     /locus_tag="ACIUS0_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGB0220664.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACIUS0_003002"
     CDS             complement(2551..2802)
                     /gene="yqgD"
                     /locus_tag="ACIUS0_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGB0220665.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="ACIUS0_003003"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="ACIUS0_003003"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGB0220666.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="ACIUS0_003004"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="ACIUS0_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGB0220667.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="ACIUS0_003005"
     CDS             5920..6417
                     /locus_tag="ACIUS0_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGB0220668.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="ACIUS0_003006"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="ACIUS0_003006"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGB0220669.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="ACIUS0_003007"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="ACIUS0_003007"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGB0220670.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="ACIUS0_003008"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="ACIUS0_003008"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGB0220671.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="ACIUS0_003009"
     CDS             9102..9665
                     /locus_tag="ACIUS0_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGB0220672.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="ACIUS0_003010"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="ACIUS0_003010"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGB0220673.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="ACIUS0_003011"
     CDS             complement(10257..11237)
                     /locus_tag="ACIUS0_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGB0220674.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="ACIUS0_003012"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="ACIUS0_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGB0220675.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="ACIUS0_003013"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="ACIUS0_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGB0220676.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /gene="yggU"
                     /locus_tag="ACIUS0_003014"
     CDS             12540..12830
                     /gene="yggU"
                     /locus_tag="ACIUS0_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGB0220677.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="ACIUS0_003015"
     CDS             12838..13431
                     /locus_tag="ACIUS0_003015"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGB0220678.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="ACIUS0_003016"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="ACIUS0_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGB0220679.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3532 a         3873 c         3757 g         3482 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaat
//
LOCUS       DBBBSB010000097        14505 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.097, whole genome shotgun sequence.
ACCESSION   DBBBSB010000097 DBBBSB010000000
VERSION     DBBBSB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14505
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.097"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(259..546)
                     /locus_tag="ACIUS0_003017"
     CDS             complement(259..546)
                     /locus_tag="ACIUS0_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGB0220680.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(621..764)
                     /gene="mgrB"
                     /locus_tag="ACIUS0_003018"
     CDS             complement(621..764)
                     /gene="mgrB"
                     /locus_tag="ACIUS0_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGB0220681.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            923..1162
                     /locus_tag="ACIUS0_003019"
     CDS             923..1162
                     /locus_tag="ACIUS0_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGB0220682.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(1305..2096)
                     /gene="kdgR"
                     /locus_tag="ACIUS0_003020"
     CDS             complement(1305..2096)
                     /gene="kdgR"
                     /locus_tag="ACIUS0_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGB0220683.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2273..3646
                     /locus_tag="ACIUS0_003021"
     CDS             2273..3646
                     /locus_tag="ACIUS0_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220684.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(3692..4573)
                     /gene="htpX"
                     /locus_tag="ACIUS0_003022"
     CDS             complement(3692..4573)
                     /gene="htpX"
                     /locus_tag="ACIUS0_003022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGB0220685.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4765..6813)
                     /gene="prc"
                     /locus_tag="ACIUS0_003023"
     CDS             complement(4765..6813)
                     /gene="prc"
                     /locus_tag="ACIUS0_003023"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGB0220686.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6833..7531)
                     /gene="proQ"
                     /locus_tag="ACIUS0_003024"
     CDS             complement(6833..7531)
                     /gene="proQ"
                     /locus_tag="ACIUS0_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGB0220687.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7628..8125)
                     /gene="msrC"
                     /locus_tag="ACIUS0_003025"
     CDS             complement(7628..8125)
                     /gene="msrC"
                     /locus_tag="ACIUS0_003025"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGB0220688.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8255..9538
                     /gene="yebS"
                     /locus_tag="ACIUS0_003026"
     CDS             8255..9538
                     /gene="yebS"
                     /locus_tag="ACIUS0_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGB0220689.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9507..12127
                     /gene="yebT"
                     /locus_tag="ACIUS0_003027"
                     /pseudo
     CDS             9507..12127
                     /gene="yebT"
                     /locus_tag="ACIUS0_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            12207..13646
                     /gene="rsmF"
                     /locus_tag="ACIUS0_003028"
                     /pseudo
     CDS             12207..13646
                     /gene="rsmF"
                     /locus_tag="ACIUS0_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            13764..14000
                     /locus_tag="ACIUS0_003029"
     CDS             13764..14000
                     /locus_tag="ACIUS0_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGB0220690.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            14105..14296
                     /locus_tag="ACIUS0_003030"
     CDS             14105..14296
                     /locus_tag="ACIUS0_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGB0220691.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(14321..>14505)
                     /locus_tag="ACIUS0_003031"
     CDS             complement(14321..>14505)
                     /locus_tag="ACIUS0_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HGB0220692.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         3457 a         3709 c         3531 g         3808 t
ORIGIN      
        1 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
       61 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
      121 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
      181 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
      241 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
      301 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
      361 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
      421 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
      481 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
      541 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
      601 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
      661 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
      721 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
      781 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
      841 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
      901 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
      961 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     1021 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     1081 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     1141 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     1201 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     1261 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     1321 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     1381 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     1441 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     1501 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     1561 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     1621 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     1681 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     1741 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     1801 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     1861 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     1921 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     1981 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     2041 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     2101 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     2161 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     2221 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     2281 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     2341 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     2401 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     2461 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     2521 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     2581 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     2641 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     2701 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     2761 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     2821 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     2881 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     2941 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     3001 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     3061 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     3121 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     3181 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     3241 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     3301 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     3361 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     3421 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     3481 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     3541 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     3601 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     3661 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     3721 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     3781 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     3841 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     3901 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     3961 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     4021 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     4081 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     4141 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     4201 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     4261 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     4321 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     4381 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     4441 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     4501 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     4561 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     4621 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     4681 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     4741 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     4801 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     4861 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     4921 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     4981 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     5041 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     5101 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     5161 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     5221 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     5281 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     5341 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     5401 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     5461 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     5521 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     5581 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     5641 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     5701 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     5761 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     5821 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     5881 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     5941 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     6001 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     6061 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     6121 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     6181 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     6241 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     6301 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     6361 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     6421 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     6481 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     6541 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     6601 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     6661 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     6721 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     6781 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     6841 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     6901 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     6961 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     7021 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     7081 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     7141 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     7201 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
     7261 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
     7321 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
     7381 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
     7441 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
     7501 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
     7561 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
     7621 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
     7681 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
     7741 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
     7801 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
     7861 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
     7921 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
     7981 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
     8041 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
     8101 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
     8161 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
     8221 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
     8281 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
     8341 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
     8401 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
     8461 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
     8521 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
     8581 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
     8641 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
     8701 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
     8761 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
     8821 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
     8881 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
     8941 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
     9001 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
     9061 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
     9121 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
     9181 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
     9241 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
     9301 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
     9361 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
     9421 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
     9481 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
     9541 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
     9601 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
     9661 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
     9721 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
     9781 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
     9841 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
     9901 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
     9961 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    10021 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    10081 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    10141 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    10201 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    10261 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    10321 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    10381 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    10441 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    10501 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    10561 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    10621 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    10681 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    10741 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    10801 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    10861 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    10921 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    10981 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    11041 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    11101 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    11161 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    11221 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    11281 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    11341 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    11401 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    11461 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    11521 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    11581 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    11641 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    11701 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    11761 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    11821 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    11881 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    11941 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    12001 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    12061 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    12121 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    12181 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    12241 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    12301 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    12361 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    12421 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    12481 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    12541 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    12601 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    12661 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    12721 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    12781 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    12841 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    12901 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    12961 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    13021 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    13081 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    13141 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    13201 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    13261 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    13321 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    13381 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    13441 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    13501 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    13561 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    13621 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    13681 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtctt actacaatca
    13741 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    13801 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    13861 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    13921 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    13981 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    14041 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    14101 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    14161 tgtggatgtc tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg
    14221 ccatggcagt tgttttccga ttgaggattt tatagatggt ttctggcaac cagcacagga
    14281 gtatggtgat ttttaattat tgcagttgca caagagtcag ttagccccca aagacagcac
    14341 cggtatcaat ataatgcagg ttgccaatat ccacgcgatg tcgcaacggt gtatgaccaa
    14401 accagaaatg atcagcacct gtaattccct gcccttttgg gcgttcacct aatcgcgagc
    14461 ggctccacaa gacctgatgc aaatcaacgt ccttttgcca ttcat
//
LOCUS       DBBBSB010000098        14487 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.098, whole genome shotgun sequence.
ACCESSION   DBBBSB010000098 DBBBSB010000000
VERSION     DBBBSB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.098"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..445)
                     /locus_tag="ACIUS0_003032"
                     /pseudo
     CDS             complement(1..445)
                     /locus_tag="ACIUS0_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(420..1220)
                     /gene="otsB"
                     /locus_tag="ACIUS0_003033"
     CDS             complement(420..1220)
                     /gene="otsB"
                     /locus_tag="ACIUS0_003033"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGB0220693.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1387..2373)
                     /gene="araH"
                     /locus_tag="ACIUS0_003034"
     CDS             complement(1387..2373)
                     /gene="araH"
                     /locus_tag="ACIUS0_003034"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGB0220694.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2388..3902)
                     /gene="araG"
                     /locus_tag="ACIUS0_003035"
     CDS             complement(2388..3902)
                     /gene="araG"
                     /locus_tag="ACIUS0_003035"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGB0220695.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3972..4961)
                     /gene="araF"
                     /locus_tag="ACIUS0_003036"
                     /pseudo
     CDS             complement(3972..4961)
                     /gene="araF"
                     /locus_tag="ACIUS0_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5758..6261
                     /locus_tag="ACIUS0_003037"
     CDS             5758..6261
                     /locus_tag="ACIUS0_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGB0220696.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6341..6592)
                     /gene="yecJ"
                     /locus_tag="ACIUS0_003038"
     CDS             complement(6341..6592)
                     /gene="yecJ"
                     /locus_tag="ACIUS0_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGB0220697.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6707..6793)
                     /gene="azuC"
                     /locus_tag="ACIUS0_003039"
     CDS             complement(6707..6793)
                     /gene="azuC"
                     /locus_tag="ACIUS0_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGB0220698.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7056..7379
                     /locus_tag="ACIUS0_003040"
     CDS             7056..7379
                     /locus_tag="ACIUS0_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGB0220699.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7550..8047
                     /gene="ftnA"
                     /locus_tag="ACIUS0_003041"
     CDS             7550..8047
                     /gene="ftnA"
                     /locus_tag="ACIUS0_003041"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGB0220700.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8084..8323)
                     /locus_tag="ACIUS0_003042"
     CDS             complement(8084..8323)
                     /locus_tag="ACIUS0_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGB0220701.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8513..9724
                     /gene="tyrP"
                     /locus_tag="ACIUS0_003043"
     CDS             8513..9724
                     /gene="tyrP"
                     /locus_tag="ACIUS0_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGB0220702.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9786..10451)
                     /gene="yecA"
                     /locus_tag="ACIUS0_003044"
     CDS             complement(9786..10451)
                     /gene="yecA"
                     /locus_tag="ACIUS0_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGB0220703.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10647..10733)
                     /locus_tag="ACIUS0_003045"
     tRNA            complement(10647..10733)
                     /locus_tag="ACIUS0_003045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10697..10699),aa:Leu,seq:taa)
     gene            complement(10746..10819)
                     /locus_tag="ACIUS0_003046"
     tRNA            complement(10746..10819)
                     /locus_tag="ACIUS0_003046"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10785..10787),aa:Cys,seq:gca)
     gene            complement(10874..10949)
                     /locus_tag="ACIUS0_003047"
     tRNA            complement(10874..10949)
                     /locus_tag="ACIUS0_003047"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10914..10916),aa:Gly,seq:gcc)
     gene            complement(11101..11649)
                     /gene="pgsA"
                     /locus_tag="ACIUS0_003048"
     CDS             complement(11101..11649)
                     /gene="pgsA"
                     /locus_tag="ACIUS0_003048"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGB0220704.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11706..13538)
                     /gene="uvrC"
                     /locus_tag="ACIUS0_003049"
     CDS             complement(11706..13538)
                     /gene="uvrC"
                     /locus_tag="ACIUS0_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGB0220705.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13535..14191)
                     /gene="uvrY"
                     /locus_tag="ACIUS0_003050"
     CDS             complement(13535..14191)
                     /gene="uvrY"
                     /locus_tag="ACIUS0_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGB0220706.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14333..14404
                     /locus_tag="ACIUS0_003051"
                     /pseudo
     CDS             14333..14404
                     /locus_tag="ACIUS0_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         3652 a         3737 c         3333 g         3765 t
ORIGIN      
        1 aaaccaatgc gattattcac cccacgtttg cgtaattcat gcgcaaaggg caacaggtga
       61 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
      121 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc
      181 cgataatgaa aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
      241 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cttttttagc ggctgatcct
      301 cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca
      361 gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt
      421 tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt
      481 aatgcggcgg ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat
      541 gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact
      601 gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaaa
      661 ggagcttcct gcataaaggc tgcaatcgct tcacctttac tggtacctct cggtttgatc
      721 tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc
      781 gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc
      841 atccctttcg cctccagctc cgcgccggga tactgagcga tgaccgtatg cagttgcacg
      901 ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca
      961 cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca
     1021 agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt
     1081 agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt
     1141 tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt
     1201 tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt gttgcgcaga
     1261 ccgcgcttta cagacatgta agtatagaca gtgtgacgga ggtcgccatt tgcaacgaag
     1321 aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaagtt gttgtggaaa
     1381 aaagcatcag acagtgcgct tcgctttttg cttgtaacgg tcgaagatca ccgctgccag
     1441 caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca ggttcatggc
     1501 gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt ttccgatgcc
     1561 acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct cataaccaat
     1621 cgacgtcatt ggctgcccac tagtcatacg tgaagccaga ataattccgg ctatcgcaga
     1681 taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta cacccgccag
     1741 acgcgcagcc tcttcgttcc cgccaatcgc cagggtatta cgaccaaagg tggttttatt
     1801 cagcagcaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg caggcagacc
     1861 gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga ccgctttacc
     1921 gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg tcgtgatcag
     1981 agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga gaacgcccag
     2041 caacaaaccc gctgccacgc caatccacag gctttcagtc aggttgataa ccaccgccgt
     2101 ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac cggaagcaag
     2161 gcagaacaac atgccacacg ccaccatccc cgacatggaa attgccaggc ccaacccttt
     2221 catattaatg aaggtggcaa aatttgggac aaaaatggca caggcgataa agagcaccgc
     2281 aaacaccacc agcatgccgt actgatccca gatacgcccg aagctgaatg acgacttagg
     2341 tgcgccagac cccgatgtag aaacagaaga catcatactc tccttactca ggcaactgcc
     2401 tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg ctcgtgtaac
     2461 aattcaccgg cgatttcgcc ttcccgcatc accacaatcc ggtcggcaac gccgaggact
     2521 tcaggtaagt cgctggaggc aaacagcacc gccacgccct gcgccgccag cgcataaatt
     2581 acgttatata tttcgtgctt agcgccaaca tcaatgccgc gcgtaggttc atccagcaaa
     2641 atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg atttccgcct
     2701 gagagattca tgatcagttg ctccgcgccc ggcgttttga tgttgagcga acgaatgtgc
     2761 tgatcggcat tgttttcttc ccaactgttg ttgattacac aaccgccgag cacatgtttg
     2821 cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat gccttccgct
     2881 ttgcgagctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg tttacgaata
     2941 tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc gccaaacaag
     3001 cctttcatta attcgctacg ccccgcccct accagaccaa acagcccaac aatttcacca
     3061 ctgcgaaccg ccagacttat tggcgtacgc acgcctagtg ctttcacagc atcaagacgc
     3121 aggcgctcct cgccataact acgaggttgc cagccgtaga tatcgccaat gtcgcgcccg
     3181 accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa ggttttgaca
     3241 taacgtccat ctttaaagac agtgatggca tcgctgaggg caaatatttc ttccatacgg
     3301 tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac gcggaaaaga
     3361 ttgccgattt cgcgggcaga aagggagctg gttggctcat caaaggcgat aattttagcg
     3421 ttacgcgcca gcgctttggc gatttcaacc atctgccact gaccaatggc gagatatttc
     3481 agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc cgcctcataa
     3541 ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag atagatgttt
     3601 tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaataatcgc cacacccgcg
     3661 ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac tacagaaccc
     3721 gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc tgcgccattt
     3781 tcacccatca acgcatgaac ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc
     3841 ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga gatacggggt agactgttgc
     3901 ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca gaggggaatt
     3961 tttccggcaa attacttacc gcctaaacct tttttctcca gttcttcttt aaagttgtca
     4021 cgcgtgatca gtaccacgtc tgtaacttcg gtaaattttg gcgtttcaac gtcttttgct
     4081 acccagttgt aaagcatttc gctggattta tagccatgta cgtccgggct tggcagcagg
     4141 gaaccgtaga agccggttgc ctgtgcttta gacagttcgc ttactgcatc cacaccgtta
     4201 ataccaatac cgatgatatc ggctgcttta aagccctgac cttcggttgc acgtacgccg
     4261 ccaagtacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc cggatgttga
     4321 accagcattg agttggcagc gtcaaatgcc cccgggatgt cgttagattt ggtaggtacc
     4381 tgataaattt gtttttccgg gaatcctgca gctttcagtg cgtccataga acccgttgta
     4441 cggcggcggg cggtatccag ttcgttggcg gtaatcgcca tcactgcgct ttctttgaca
     4501 tcccagccac gtttctgcat ctctttatac agttcctagc cctgacgttc gccaatttta
     4561 gtcgccgcca tcatcaccag aggaacggta tccattggct tacctttggc gttaacaaac
     4621 tggtcatcca ctgcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag
     4681 ccgagtttcg ggtccggagt acaaataacg aaaccttttg cgccactggc tgccaggctg
     4741 tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct
     4801 aaatccttcc cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt
     4861 tgcttcacca gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct
     4921 gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt
     4981 gtcgttttgt gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat
     5041 taccttttat ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct
     5101 ccataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg
     5161 aaaagacata aggaaaagcc aatttgtcag acaaattgtt gaatgcacag cagattaatc
     5221 cataagatta gcctggaaat ccttgttgtc ttcggtaccc atgcgggatg tcttcttttt
     5281 aaccagtcaa taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccacaa
     5341 ctcaaagatg tggaaaatgc gcgtcattca tttcgccatt aattatcact gtgctcatta
     5401 attaacagaa cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat
     5461 catcacttca tcatgaattc aaattcatcg attaatatca acaagataca aaaagcacta
     5521 tcattaaaat tcattgcatt tacattgatt tcaccaatga aatgtaaaaa tatataaact
     5581 tgatgattta agcattttct tataaccgtt cagacgttat tcttatttca gatcatcgtc
     5641 agaattgact ccacgatcac atttcggacc ggcagaaagg aattattctg caaacagtaa
     5701 ttatggtgtt ttgatttatc ttgcacctct ccacttctgg atataaggat attaggtatg
     5761 gcaaccgctg gaatgcttct caaactcaac tctcaaatga accgcgagtt ttacgcatcc
     5821 aatctctacc ttcacctgag taactggtgt tctgaacaga gtctgaacgg caccgccact
     5881 ttccttcgcg cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt taactttatg
     5941 aagagtgtcg gcgctacccc catcgttaaa gccattgatg ttccaggtga aaaactgaac
     6001 tctctggaag aactgttcca aaaaacgatg gaagaatacg agcaacgttc aagcactctg
     6061 gcgcagttag ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc
     6121 gatctggaaa aagaacagca gcatgatggc ctgttgctgc aaaccattct tgatgaagtg
     6181 cgcagtgcga aacttgcggg tatgtgccct gtgcagaccg accaacatgt tctgaatgtc
     6241 gtgtcacacc agctgcattg atcatacatc ggcgctaata cattgcgccg atgaagtttt
     6301 tgagaaaccg ctgcctcatc tgtttgaagc agcggttttt ttaatgggat tcaccctgtg
     6361 gggtaaactt gagttcaatc agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt
     6421 ctgcggcagc gggatgatga gtaaagtcga tattcttcag ctgactggac attttttcac
     6481 gaacttcaac gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc
     6541 aagctaccac atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact
     6601 cctgatagca ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat
     6661 ccaccgtcat caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat
     6721 tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt
     6781 tgcgcagttt catcgcggca ctccgaccat ttgttataca ggtgttattg tctttgcgct
     6841 tataatatga caaccatcac aaaaatcaat ctttatgtga tacaaatcac ataaataccc
     6901 ctttaatgtt ataaaaatga taatcaaaaa acagcccccc tatttctgac acctacagat
     6961 ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt
     7021 aaaatagtgc tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt
     7081 cattaataac gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg
     7141 cggcaacagg tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg
     7201 atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc tatacccacg
     7261 cgatcccctt tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga
     7321 atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa
     7381 tattttaatc agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc
     7441 ccattcgtat ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca
     7501 aattatagtg acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc
     7561 agaaatgatt gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta
     7621 tcagcaaatg agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg
     7681 ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac
     7741 cggcaattta ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga
     7801 tgaattattc caggaaacct ataaacacga acaattaatc acccagaaaa ttaacgaact
     7861 ggctcatgct gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt
     7921 ttctgagcag catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc
     7981 aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca
     8041 aaactaatgc tctccggcgg caggtttctg ccgccgtttc aaatcagtga cggcaaatct
     8101 tactctgatc ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt gctgccagaa
     8161 acgctaccag ctctcccgct gtcacccctt ctgccgagca cgtgtgaaaa cgtgcctgtt
     8221 caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt
     8281 caatcatcat atttaacact tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa
     8341 agcctagcgt agcgattacc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt
     8401 tattgtacat ttatatttac acaatatgta acgtcggttt gacgaagcag ccgttatgcc
     8461 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     8521 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     8581 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     8641 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     8701 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     8761 gggcttcagt atgatgttct taatgtatgc tctgactgca gcatacatca gcggtgccgg
     8821 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     8881 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     8941 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     9001 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     9061 actgtctgca atcccggtga tttttacgtc gtttggtttt cacggtagcg tgccgagtat
     9121 tgtcagctat atggatggca acattcgtaa gctacgctgg gtgtttataa tcggtagtgc
     9181 catccccctg gtggcatata ttttctggca ggtggcgacg cttggcagca ttgattcaac
     9241 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     9301 cgaaatggtg gcctctccgc atgttgagct ggcggtgcat ttatttgctg atttagccct
     9361 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttgtttca
     9421 gcgttcaaat accgttggtg gacgattgca aactggtgca attacctttc tgccgccgtt
     9481 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     9541 gctggcggta ctggcattga ttatcccttc actattgacc tggcaaagca gaaagcacaa
     9601 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     9661 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     9721 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatgtg
     9781 acctcttaat gcaggcagca ctgcttaaat ttcttaccac tgccgcacgg acaaggatcg
     9841 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     9901 gccatccagt atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     9961 aacgcttctg gcgacatttt ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca
    10021 atcgcctcca gcgctggttt taaagaatcg ggcaacgttg accagtcaga aagtgccacg
    10081 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
    10141 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
    10201 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccaacgc
    10261 ggcacatagt cagccccacc ccacacggca accagccact gttctggttc aatctcttgc
    10321 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
    10381 tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
    10441 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
    10501 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
    10561 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttatta
    10621 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
    10681 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
    10741 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
    10801 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
    10861 atgtctttta aactggagcg ggaaacgaga ctcgaactcg agacctcgac cttggcaagg
    10921 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
    10981 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
    11041 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
    11101 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
    11161 cagtaccgca gccacaaaga aaagtgcaat accgacgtac tcaacccaaa tgttcggacg
    11221 ccacagcagc caggccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
    11281 gacggccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
    11341 aatttcacgg gcgatcatcg ttgccgccgg taatgtcacc caccagctgt gataatgctc
    11401 ggttaccagc accatggcga tagccacgag aactttatct gccacagggt caaggaaagc
    11461 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
    11521 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
    11581 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
    11641 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
    11701 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
    11761 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
    11821 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
    11881 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
    11941 atatgctgga taacatgcag cgcgggtgaa tctggcggca aactaaatcc ctcaccttcc
    12001 ggctcaaaga acagcgtttc cagtccagcc ttacgatctg ctcctttggc aacgccaagt
    12061 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
    12121 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
    12181 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
    12241 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
    12301 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
    12361 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
    12421 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
    12481 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
    12541 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
    12601 aaggtgcgca tctggctgcc ttgtaaatag aactgaccta cgaaggtttc taccacctcg
    12661 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
    12721 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
    12781 tcaaggtcgt cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga
    12841 atttggtcgc gaatacatgc cgcttcttca aactccagat tctggctggc agtttccata
    12901 cggctaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
    12961 tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
    13021 cgccctatct ggtattgcag acacggacgc gagcgattgc gataaacact attttcgcac
    13081 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
    13141 gggaacgggc cgaaatattc acctttggca tgctttgcac cacgatgcat cgccagacgc
    13201 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
    13261 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
    13321 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
    13381 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
    13441 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
    13501 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
    13561 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
    13621 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
    13681 agatctcatt gaccttctgg cccttagtga tcatcagcat aatctgcaat tcacgttcag
    13741 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
    13801 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
    13861 cttcctgcgg agccgcgcct ttgctgaggt agccagcagc acctgcctgc atgactttcg
    13921 ctggtaaagg gttttctgta tggaccgtaa gcatgatgat tttaacatca gctgtggaac
    13981 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
    14041 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctctc
    14101 cgacgacttt tataccctta atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
    14161 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
    14221 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
    14281 tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac
    14341 gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctacgatga aatgtgacaa
    14401 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
    14461 ccgaacaata cgggcaaaaa acattca
//
LOCUS       DBBBSB010000099        14479 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.099, whole genome shotgun sequence.
ACCESSION   DBBBSB010000099 DBBBSB010000000
VERSION     DBBBSB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.099"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..116
                     /locus_tag="ACIUS0_003052"
     CDS             <1..116
                     /locus_tag="ACIUS0_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220707.1"
                     /translation="TISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(113..379)
                     /locus_tag="ACIUS0_003053"
     CDS             complement(113..379)
                     /locus_tag="ACIUS0_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGB0220708.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            653..4177
                     /gene="nifJ"
                     /locus_tag="ACIUS0_003054"
     CDS             653..4177
                     /gene="nifJ"
                     /locus_tag="ACIUS0_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGB0220709.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4544..5673
                     /gene="ompN"
                     /locus_tag="ACIUS0_003055"
                     /pseudo
     CDS             4544..5673
                     /gene="ompN"
                     /locus_tag="ACIUS0_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5814..6248
                     /gene="uspF"
                     /locus_tag="ACIUS0_003056"
     CDS             5814..6248
                     /gene="uspF"
                     /locus_tag="ACIUS0_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGB0220710.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6424..7359
                     /gene="ttcA"
                     /locus_tag="ACIUS0_003057"
     CDS             6424..7359
                     /gene="ttcA"
                     /locus_tag="ACIUS0_003057"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGB0220711.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACIUS0_003058"
     CDS             complement(7488..8855)
                     /gene="dbpA"
                     /locus_tag="ACIUS0_003058"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGB0220712.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACIUS0_003059"
     CDS             complement(8885..9058)
                     /gene="ynaL"
                     /locus_tag="ACIUS0_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGB0220713.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACIUS0_003060"
     CDS             complement(9333..10316)
                     /gene="zntB"
                     /locus_tag="ACIUS0_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGB0220714.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACIUS0_003061"
     CDS             complement(10330..10377)
                     /gene="ydaN"
                     /locus_tag="ACIUS0_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGB0220715.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10571..11803
                     /gene="dgcM"
                     /locus_tag="ACIUS0_003062"
                     /pseudo
     CDS             10571..11803
                     /gene="dgcM"
                     /locus_tag="ACIUS0_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACIUS0_003063"
                     /pseudo
     CDS             complement(11824..12387)
                     /gene="smrA"
                     /locus_tag="ACIUS0_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12641..14095)
                     /locus_tag="ACIUS0_003064"
     CDS             complement(12641..14095)
                     /locus_tag="ACIUS0_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HGB0220716.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14092..>14479)
                     /locus_tag="ACIUS0_003065"
     CDS             complement(14092..>14479)
                     /locus_tag="ACIUS0_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGB0220717.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3417 g         3550 t
ORIGIN      
        1 acaccattag cgaaatgctg aaagaaggtg cacaagtgga tctgaccgcg aaccagttaa
       61 cgctggcgac cgcgaaacag acattaactt ataagctggc ggatttaatg aattaatagc
      121 tgccacagct cccggcggca agtgactgtt cgctacagcg tttgccgttg ggtaatgcac
      181 acatcccaat cgccgtacca tccagttgac gggcaacaga aagcgaaccg ccgatcattg
      241 cacaatttgc ttctccacta ctggacatcg acgcttttaa acttggcgct acgtgcgccg
      301 cagtcgcctg ctgaacaggc tcactactac acgccgacaa caataaagcg gcacacccta
      361 cccaaaacgc tgctcgcatc tcttcttcct ctgatcttca agccaaacga caccgccata
      421 aataataggc agcacagagg gcggcgtcga gagctgtcct gcgcgttgcc ccgcattttt
      481 acttttttat ggctattttt ttgccctctg tttgatcaaa acattcatta ctctgatgtg
      541 ggggacacaa aagcgaaaat gcagaagaaa gccatttgct aaaattgaag gattactact
      601 gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg taagggtgtc atatgattac
      661 tattgacggt aatggcgcgg ttgcttcggt gacgtttcgc atcagtgaag ttatcgccat
      721 ctaccctatt acccccagtt ccacgatggc agaacaggct gatgcctggg ccggaaacgg
      781 cttgaagaac gtttggggag acacaccacg cgtggttgaa atgcagtcgg aagcgggtgc
      841 tatcgctacc gtgcatggcg ctttgcagac gggtgccctt tcaacatcgt ttacgtcatc
      901 gcagggtttg ctgctgatga tcccgacgct gtacaaactg gcaggcgaac tgacgccatt
      961 tgtcctgcat gtagcggcac gtaccgttgc cacacatgca ctttctattt ttggcgatca
     1021 ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg ttgtgtgcgg ctaacgtcca
     1081 ggaagcgcaa gactttgctc tcatttcgca catcgcaacg ctgaagagcc gcgtgccatt
     1141 tattcatttc tttgatggtt ttcgcacgtc ccacgaaatc aataaaattg tcccgctggc
     1201 cgatgacact attcttgatc tcatgccgca ggccgaaatt gatgctcatc gcgcccgggc
     1261 gctcaacccg gaacatccgg tgatccgcgg tacgtccgcc aatcctgaca cttatttcca
     1321 gtctcgcgaa gccaccaatc cctggtacaa cgcggtctat gaccatgttg aacaggcgat
     1381 gaatgatttc gctgccgcga caggtcgtca gtatcagccg tttgagtatt acgggcatcc
     1441 gcaagcggaa cgggtgatta tcctgatggg ctctgccatt ggcacctgtg aagaagtggt
     1501 tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg aaagttcgtc tgtaccgtcc
     1561 cttctccgcc aaacacttgc tgcaagctct gccgggatcc gtacgcagcg tggcggtact
     1621 ggacagaacc aaagaacccg gtgcccaggc agaaccgctc tatctggatg tgatgaccgc
     1681 actggcagaa gcctttaata atggtgagcg cgaaaccctg ccccgtgtca ttggtgggcg
     1741 ctatggtctt tcatccaaag aatttggccc ggactgtgta ctggcggtat ttgccgagct
     1801 caacgcggct aaacctaaag cacgctttac ggttggtatt tacgatgatg tgaccaatct
     1861 gtcactgccg ttgccggaaa acactctgcc aaactcggcg aaacttgaag ccttgtttta
     1921 tggccttggt agtgatggca gcgtttccgc aaccaaaaac aatatcaaga ttatcggtaa
     1981 ttccacgccg tggtacgcac agggctattt tgtttacgac tccaaaaaag cgggcggcct
     2041 gacggtttct caccttcgcg tgagcgaaca gccgattcgt tccgcttatc tcatttccca
     2101 ggctgatttt gttggctgcc accagttgca gtttatcgat aaatatcaga tggctgagcg
     2161 tttaaaacct agcggcattt tcctgcttaa tacgccgtac agcgcagatg aagtgtggtc
     2221 gcgcttgccg caggaagttc aggccgtgtt aaaccagaaa aaagcgcgct tctatgtgat
     2281 taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc cgtattaata ccgtcatgca
     2341 gatggctttt ttccatctga cgcaaattct gcctggcgat agcgccctcg cagaattgca
     2401 gggtgcgatt gccaaaagtt acagtagcaa aggtcaggat ctggtggagc gcaactggca
     2461 ggctctggcg ctggcgcgtg aatccgtaga agaagttccg ttgcaaccgg taaatccgca
     2521 cagcgccaat cgaccgccag tggtttccga tgccgcccct gatttcgtga aaaccgtaac
     2581 cgcggcgatg ctcgccgggc ttggtgatgc cctccccgtt tcggcgctgc cgccagacgg
     2641 cacctggccg atgggcacca ctcgctggga aaaacgcaat atcgccgaag agatccccat
     2701 ctggaaagag gaactctgta cccaatgtaa ccactgcgtc gccgcttgcc cacactcagc
     2761 tattcgcgca aaagtggtac cgcctgaagc gatggaaaac gcccctgcca gcctgcattc
     2821 gctagatgtg aaatcgcgtg atatgcgcgg gcagaaatat gtcttgcagg tggcaccgga
     2881 agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg gcgaaagacc gtcagaatcc
     2941 agagattaaa gccatcaata tgatgtctcg ccaggaacat gtcgaagaag agaaaatcaa
     3001 ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc aaactggaac gtattgatat
     3061 tcgtacatcg cagctgatta caccgctgtt tgaatattca ggtgcttgcc ccggttgtgg
     3121 cgagacgccg tatattaaat tgctgactca gctctatggt gaccggatgt tgatcgctaa
     3181 cgccaccggc tgttcttcaa tttatggcgg taacctgccc tctacaccgt ataccaccga
     3241 tgccaacggt cgtgggccgg catgggcgaa ctctctattt gaagataatg ccgaatttgg
     3301 ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc gtgctgcgtc tgctggatca
     3361 atttgccgat aaaatcccgg cggaattact gacggcgttg aaatcagacg ctacgccaga
     3421 ggttcgtcga gaacaggtcg cagctttacg ccagcaactc aacgatgttg ccgaagcaca
     3481 tgaacttctg cgtgacgcag atgcactggt ggaaaaatca atctggctga ttggtggcga
     3541 cggctgggct tacgatatcg gctttggcgg tctggatcat gtattgagtt tgacggaaaa
     3601 cgtcaacatt ctggtgctgg atacgcaatg ctattccaac accggtggtc aggcgtcgaa
     3661 agcgacaccg ctgggtgcag taactaagtt cggcgagcac ggcaaacgta aagcgcgtaa
     3721 agatcttggc gtcagtatga tgatgtacgg tcatgtttat gtggcgcaga tttctctcgg
     3781 cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg gaagcgtatc cgggaccatc
     3841 gctgatcatt gcttatagcc cgtgtgaaga gcatggttac gatctggctc tcagccacga
     3901 ccagatgcgc caactcactg ctaccggctt ctggccgcta tatcgctttg atccgcgtcg
     3961 tgccgatgaa ggcaaactgc cgctggcgct ggattcacgc ccgccgtcag aagcactgga
     4021 agaaacgtta cttcacgagc agcgtttccg tcgtctgaat tcgcagcagc cagaagtggc
     4081 agaacagtta tggaaagatg ctgcagctaa tttgcaaaaa cgctatgact tcctagcaca
     4141 aatggccgga aaagcggaaa aaagcaacac cgattaaatg ctctggataa ggattatcca
     4201 attctaaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt tggaaaatca
     4261 gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca tataacccgc
     4321 gcagaataac gtataggaaa ttaaaagtat aatttttatt ttgtatgtat tatcttcgat
     4381 acattcacca gacttattat catttcagag gaattatttg attaaggttt tacttaaggc
     4441 gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt cactccccga
     4501 aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa gaaaagtact
     4561 gacactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg aagtttataa
     4621 taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt atttttctga
     4681 taattcagcg aaagatggcg accagagcta tgcgcgtctg ggttttaaag gcgaaaccca
     4741 aattaacgat caacttactg gctacggtca atgggaatac aatattcagg caaacaacac
     4801 tgaatcttca aaaaaccagt catggacccg tctggcattt gccggactga aatttgcaga
     4861 ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg aaggctggac
     4921 cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact ttatgactgg
     4981 tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg taaatggtct
     5041 gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac
     5101 caataacgaa cgcgatgttc gtcatgaaaa cggtgacggc tggggtcttt ccacaacata
     5161 tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc gcaccaatga
     5221 ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg ctgggctaaa
     5281 atacgatgct aacaatattt acctagcaac catgtattca gaaacgcgta atatgacccc
     5341 gtttggcgac agcgattatg cagttgcgaa caaaacccag aactttgaag ttactagtac
     5401 cagtttgatt tttgtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
     5461 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
     5521 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
     5581 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
     5641 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
     5701 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
     5761 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
     5821 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
     5881 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
     5941 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
     6001 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
     6061 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
     6121 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
     6181 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
     6241 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
     6301 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
     6361 gctgagcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     6421 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     6481 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     6541 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     6601 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     6661 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     6721 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     6781 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     6841 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     6901 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     6961 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     7021 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     7081 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     7141 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     7201 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     7261 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     7321 ttggatgagt tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     7381 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatctggca
     7441 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     7501 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     7561 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     7621 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     7681 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     7741 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     7801 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     7861 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     7921 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     7981 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgttgctc cagatcgccg
     8041 tgtaacgaca atgcactttg ctctacttca ttcagcgcgt cgcagacagc ctggcaatct
     8101 tttttggtat tgcaaaacac cacacaagag gatggctgat gcaagcttaa taaccgttgc
     8161 aacagaggaa ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa
     8221 gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg
     8281 gcgatggctt ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa
     8341 cggatgacat catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc
     8401 atcaccaaag tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa
     8461 cgacccggcg ttgccacgat aatatgcggc gcatgttgca gcgaatcacg ctgcataccg
     8521 aacggttgac caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga
     8581 cgcaattcac ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg
     8641 gtttgaaata gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg
     8701 ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg
     8761 gcggcctgca ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg
     8821 ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc
     8881 aaagttagca ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt
     8941 cttcatcggg tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa
     9001 caggatcgcc aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat
     9061 ttccctccgg tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa
     9121 aaagccgact catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa
     9181 aaggaagtaa gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag
     9241 ggagatattg ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt
     9301 ttgtatacag acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat
     9361 aaagcaacac caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc
     9421 cacccgccgc cagggatccc accaaggttg acgccaaata acccggtcag aaaggtactg
     9481 ggtaaaaaga ccattgccat caacgacatt gtataggtac gacgagctaa attttcctgc
     9541 atcacctgag cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg
     9601 tcaaggccgc gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc
     9661 atccacggca aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag
     9721 cgacgcatca caattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc
     9781 tgatcaagga gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattca
     9841 ctggaatgat cggtcaacgc atcgcacaca tccaccagcc atcccccgca atcggtcgga
     9901 cccgtgccct cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg
     9961 gtcgaaacaa ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt
    10021 tcatcggtgc tgccgtttat acagcgcaat gtaatcagcg tgccttcacc gaaacggctg
    10081 actcggggcc gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt
    10141 gttgtcgcca gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga
    10201 tgcgcttcat caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc
    10261 atccaggcaa atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccactgtg
    10321 cctccatcat caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc
    10381 atcaccccat taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa
    10441 aatgcttttc cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt
    10501 atctgatcat atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa
    10561 cgggatctca atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg
    10621 gattgtttcg ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat
    10681 gcaagacctc acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga
    10741 actgccgaaa tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt
    10801 tcagcgtaaa gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga
    10861 aacagaaccg gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc
    10921 cagtcgctcg gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac
    10981 taactctgca ccaatgttgt tgattgaccc gtcacgagat ggacaaatcg tcgatgctaa
    11041 cctcgccgcg ctcaatttct atggttataa ccatgaaacg atgtgccaga aacataccta
    11101 ggaaataaat atgcttgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc
    11161 cggcggtcat aaccctttga attttgttca taaactggcg gatggatcta ctcgtcatgt
    11221 gcagacctat gccggaccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca
    11281 tgatattact gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc
    11341 gatgaccgga ttactgaatc ggcgacagtt ttatcacatt acggaaccag gccaaatgca
    11401 gcatctcgcc atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca
    11461 cattaacgat ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgccc
    11521 cctcgaaagt tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt
    11581 cttattgcta ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgcgt
    11641 aagcgtggca aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc
    11701 gcatcacgaa ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta
    11761 tcgggcgaaa aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg
    11821 agatcaacga ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt
    11881 acgtagtgcg acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc
    11941 ctgaacatca tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg
    12001 tgatttatct tcccgccctt taccatgaat aatcagcaca ttacgcaaac catccgccag
    12061 ggcttgttga atgaaactga acaccatttt gcggcactct tccaccggct ggcgcaaaag
    12121 attcaggctc gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgcta
    12181 caacccttcc tgccgaaact ccagcggctg acttagcggg atgatgtcga gaaatccggt
    12241 agtgaggaaa ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt
    12301 tggatgccag tggacatctg tagcacgttt cagcggctgg acatcttcca tggcgtcaag
    12361 aaacagcgat ttgtcgtcaa ggttcatgta acctcctatg caccttcaca cgtcgtgatt
    12421 cagacgggtt aattgttcat acttactgca tcgttccctt cacagattga aacaatactc
    12481 tacttcatac ctgtgagtat ctgcgcaggc ggggcatttt atactagcct gttagcagta
    12541 tcgaacagtt tagcccgagt ttaattcagc acaggctccg atcaggatga tttattcata
    12601 gcaggaacca acaaaaatgc gataaataac agaccactga ttagcagaaa ggtaccgaaa
    12661 atgccatagt ttgcgcctcc ctcagcatca attgactcaa taatttggtt gaagccagta
    12721 tttccgatgt ctggtcactt ctactgcgat taaaaaataa agcacttctg gaaatgaagt
    12781 cattcaccgt atggggaaca gaaaccattt tttcacccat tctcgaaaaa tgaaaattag
    12841 ttcgattatt gagaatagag ccacacaggg caataccaat cgcgccccca agattacgtg
    12901 ttaaattaaa tacaccagag gctaacttca gccgttcttt tggaattccc cccagcgtta
    12961 atgttacgat gggggccatc gcaaactgct gcgaaatccc tctaattgcc tgtggaaaca
    13021 gtaattcctg ccatccccat tcgtgggtta taggggtaaa aagatacatt gaaaacacaa
    13081 atccgcccag ccccgccatc aacagccatc gcagatttat ttttttactt aaccagaagt
    13141 aaaagggaac agaaaataac tggaaaatac ctgttgtgca aacggcaaaa cctatctctt
    13201 cggcatttaa tccgcgaatc tgccccaaaa acacaggtat taaatataca gtagagaata
    13261 tcccaacccc tccagaaaaa gaaaaaaaac atcccagagt gaaattttta tccttaaaag
    13321 catgaagatc cattatcgga ttggatattg taagtgtacg cgcagcaaat aaaataaaac
    13381 tcaccaatgc cagaactgat gtcagcaaaa tagtattatc atccagccat ccccagcgcg
    13441 ctccttcttc aagggtatat tccagacatc ccagagtcat ggccaacagg ataatactcg
    13501 gataatcagc aactttcagt aaagagagat caggtttatc aaaattaacc agaaaaggaa
    13561 tactcaaaac taaatatatg ccaggtaata tattgatata aaatagccat cgccagtcga
    13621 ggttatcagt gagccagccc cccagagttg gccctagcgt cggtgacagt gaggccagcg
    13681 cactgactac cgcagcagcc aaccccaact ctttaccctg gtaataaata aacgccatcg
    13741 tgaaaactaa agggatcatg gatgcgccag cagctccctg taaagcacga aacaaaatca
    13801 tgatctgaat attccatgca agtccacacg cgatactcat taaagtaaat attcccgcgg
    13861 atagagtaaa aagccaacga gtggaaaata cccgtgacag ccacccagag agcggaataa
    13921 caataatctc agcaattaaa tagctggtct gaagccaggc catttcgtct ttacctgcgg
    13981 acagaccacc acctatttca tttagggatg acgatacaat ttgaatatca agctgagcaa
    14041 taaataatcc catacacata ctggcgaaag caatatgttt acgcatagac atcatctctc
    14101 atcaacactg acggtgacgg acagtcccgg gcgaagaagt tgctttaatt cctttgcgtc
    14161 gtcaaatgca atccgtaccg ggacacgctg aacaattttg gtgaaattgc cagtggcatt
    14221 ttctatcgga atcaagctga aactggctcc tgtcgctggg gaaaggcttt caatgtgacc
    14281 gtgaaacact tcccctttga gaatatcagc acggatctca gccttcattc ctggcttcat
    14341 tcccgctatt tggttttctt tataatttgc atcaacccac aactcactga ctggtactag
    14401 tgaaactaac gatgtgccgc cttccaccca actgcctgta tgggcgctgc ggtttgcgac
    14461 aatgccatca atcggcgca
//
LOCUS       DBBBSB010000100        14317 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.100, whole genome shotgun sequence.
ACCESSION   DBBBSB010000100 DBBBSB010000000
VERSION     DBBBSB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.100"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..3051
                     /locus_tag="ACIUS0_003066"
                     /pseudo
     CDS             1..3051
                     /locus_tag="ACIUS0_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="ACIUS0_003067"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="ACIUS0_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="ACIUS0_003068"
     CDS             6768..7175
                     /locus_tag="ACIUS0_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220718.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="ACIUS0_003069"
     CDS             7717..8805
                     /locus_tag="ACIUS0_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGB0220719.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="ACIUS0_003070"
     CDS             8816..11095
                     /locus_tag="ACIUS0_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGB0220720.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="ACIUS0_003071"
     CDS             11088..12224
                     /locus_tag="ACIUS0_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGB0220721.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="ACIUS0_003072"
     CDS             12221..14224
                     /locus_tag="ACIUS0_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220722.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3547 a         3754 c         3653 g         3363 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcac cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cctggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgctg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggttggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctggtgag agtcacc
//
LOCUS       DBBBSB010000101        13964 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.101, whole genome shotgun sequence.
ACCESSION   DBBBSB010000101 DBBBSB010000000
VERSION     DBBBSB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.101"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACIUS0_003073"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACIUS0_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGB0220723.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACIUS0_003074"
     CDS             2219..2767
                     /locus_tag="ACIUS0_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGB0220724.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACIUS0_003075"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACIUS0_003075"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGB0220725.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACIUS0_003076"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACIUS0_003076"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGB0220726.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACIUS0_003077"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACIUS0_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGB0220727.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACIUS0_003078"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACIUS0_003078"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGB0220728.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACIUS0_003079"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACIUS0_003079"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGB0220729.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="ACIUS0_003080"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="ACIUS0_003080"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGB0220730.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACIUS0_003081"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="ACIUS0_003081"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGB0220731.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACIUS0_003082"
     CDS             complement(13351..>13964)
                     /gene="ssuC"
                     /locus_tag="ACIUS0_003082"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGB0220732.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3549 a         3564 c         3527 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atcggaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgct
//
LOCUS       DBBBSB010000102        13950 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.102, whole genome shotgun sequence.
ACCESSION   DBBBSB010000102 DBBBSB010000000
VERSION     DBBBSB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.102"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(193..525)
                     /gene="pemK-mt"
                     /locus_tag="ACIUS0_003083"
     CDS             complement(193..525)
                     /gene="pemK-mt"
                     /locus_tag="ACIUS0_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HGB0220733.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(527..784)
                     /gene="pemI"
                     /locus_tag="ACIUS0_003084"
     CDS             complement(527..784)
                     /gene="pemI"
                     /locus_tag="ACIUS0_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HGB0220734.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(877..1530)
                     /locus_tag="ACIUS0_003085"
     CDS             complement(877..1530)
                     /locus_tag="ACIUS0_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGB0220735.1"
                     /translation="MIQTRNQYLQFMLIMLAAWGISWGARFVMEQAVLLYGLGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2469..3326)
                     /gene="repA"
                     /locus_tag="ACIUS0_003086"
     CDS             complement(2469..3326)
                     /gene="repA"
                     /locus_tag="ACIUS0_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGB0220736.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3319..3393)
                     /gene="tap"
                     /locus_tag="ACIUS0_003087"
     CDS             complement(3319..3393)
                     /gene="tap"
                     /locus_tag="ACIUS0_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGB0220737.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(3631..3885)
                     /locus_tag="ACIUS0_003088"
     CDS             complement(3631..3885)
                     /locus_tag="ACIUS0_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGB0220738.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            complement(4125..4715)
                     /locus_tag="ACIUS0_003089"
     CDS             complement(4125..4715)
                     /locus_tag="ACIUS0_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HGB0220739.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(4753..4962)
                     /gene="hha"
                     /locus_tag="ACIUS0_003090"
     CDS             complement(4753..4962)
                     /gene="hha"
                     /locus_tag="ACIUS0_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGB0220740.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(5008..5469)
                     /locus_tag="ACIUS0_003091"
     CDS             complement(5008..5469)
                     /locus_tag="ACIUS0_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGB0220741.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(5714..5926)
                     /locus_tag="ACIUS0_003092"
     CDS             complement(5714..5926)
                     /locus_tag="ACIUS0_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGB0220742.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(6055..6615)
                     /locus_tag="ACIUS0_003093"
     CDS             complement(6055..6615)
                     /locus_tag="ACIUS0_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGB0220743.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(6718..7578)
                     /locus_tag="ACIUS0_003094"
     CDS             complement(6718..7578)
                     /locus_tag="ACIUS0_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGB0220744.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(7637..8383)
                     /gene="traX"
                     /locus_tag="ACIUS0_003095"
     CDS             complement(7637..8383)
                     /gene="traX"
                     /locus_tag="ACIUS0_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGB0220745.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(8403..13673)
                     /gene="traI"
                     /locus_tag="ACIUS0_003096"
     CDS             complement(8403..13673)
                     /gene="traI"
                     /locus_tag="ACIUS0_003096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGB0220746.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQIERDMVRDLQKEKTLGGD"
     gene            complement(13673..>13950)
                     /locus_tag="ACIUS0_003097"
     CDS             complement(13673..>13950)
                     /locus_tag="ACIUS0_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HGB0220747.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         3062 a         4171 c         3325 g         3392 t
ORIGIN      
        1 gaacttcaaa actgcgcaaa acacgcaaat acgcacaaga aagtagggct gcgcttggaa
       61 ttcggaaaat aaaccacatc aaaactactt tttagccaaa taacgtttgg gaatcaccag
      121 aatggtggga caacagcggt tttagtgccc taaatcgtac gttttcatcc agttgcccct
      181 caaaccccat gttcaagtca gaatagtgga caggcggcca agaacttcgt tcatgatagt
      241 ctccggaacc cgttcgagtc gttttccgcc ccgtgctttc atatcaattg tccggggttg
      301 atcgcaacgt acaacacctg tggtacgtat gccaacacca tccaacgaca ccgcaaagcc
      361 ggcagtgcgg gcaaaattgc ctccgctggt tacgggcaca acaacaggca ggcgggtcac
      421 gcgattaaag gccgccggtg tgacaatcag caccggccgc gttccctgct gctcatgacc
      481 tgcggtagga tcaagcgaga caagccagat ttcccctctt tccatgtcag atttcctcct
      541 gaccagtcgc cggtgcatcc agccattctc gttcttcagc tgatatttca gcattcggat
      601 cacactgtgc cagtagctca gccagtgaat attgcgggcg tctgtacggc tcaacaatca
      661 gccggccatt atcaatgacc atgccaactt cattatctgt gcccagagac agcgcattca
      721 gcagtgccgg tgggacggtc agcataactg agccgccaac cctcttcagt cgggtggtat
      781 gcattcttca cctccataaa agttatattt aaatataaca tccactaaaa aaacacacca
      841 ggctttaacg cacaatgttt aataaaaata taactttcaa ccaaacagta aacccagcgt
      901 ggttgcttcc atatgcagca ataccggcag cagcaggcca ccacttctga tcctggccac
      961 tgatgtaatc aaccccacca ggaacagttc tgccagtgtc agcaggttct gatactggct
     1021 gtgcgcggcg acgaacaaca acgacgttat cagcgccccc agccacatcg tccagcagta
     1081 ccgtgaacgg aagacgttca gcataatccc ccggaacagc gtttcctcat tcaacggggc
     1141 aaggataaag atggtcagca acgtcaggat cacgtcaggt atggacttat cggcaaaaag
     1201 tttcgtcata aatggctcag caggcagagc cagcgcctta ccgagcagaa atacaccgac
     1261 atacaccacg gccatcgcac cgaccagcca cggtacgcca acgttgcgca gctgaccaac
     1321 gaccggtagc ggagctatcc aacggcggta taccaggaaa acacacagca ggtacatcag
     1381 aacagtacca tgactgaaga acaaatagtt ttttcctaat ccataaagca gaacggcctg
     1441 ctccatgaca aatctggctc cccaactaat gccccatgca gccagcataa tcagcataaa
     1501 ctgcagatat tgattacgtg tttgaatcat tgcatcgcct gtaaattttt aacttgtcct
     1561 atttttgtca ttaccacgta tatacacatg tataacaatt cagatatcgt taccaggata
     1621 tgccgcatca gcggcatgga aggcggcact ctgttgtttc atatgataca ggagtaaaac
     1681 cgccgaagcc cggcgtaagc cggtactgat tgatagattt caccttaccc atccccagcc
     1741 ctgccagacc atacccgctt tcagccatga gagagcttct gtgcgcggtc ggagtggtcc
     1801 cgacgagggt ttacccgaag tcggggcgta tctccgcgtt agcgggccgt gagggccgct
     1861 tacgagcgtg tactgagaac ttccagcgag aagactgaca gcgatgaaga tgtagttaca
     1921 acattcataa ttaaaagcga ctctgttccg gccctttggg ccggggcggg gccgcttttc
     1981 agttatgagg gaggggcttt gtggtttcgg ttctgcgctg gaccggggtt tttctggagg
     2041 ttgtttttgt gtgttgtaac taaagtggct ccggtcgggg cccgccgctt gcggtgggag
     2101 gtgcatatct gtctgtccac aggacaggca gtgaataggt tttcttttta aatgaatgta
     2161 attaagtagt ttaaaggaga tataaacagg tgtttaaaag atacattgca ccctgtaaga
     2221 ctggcggctg gcgctttatg acatgaacgg ttgtaacctt atggggaagt cccttgcagt
     2281 taaatgtgga taagcaaaat tccccgtctg tgaggcgtgt tttgtatcga aaacaggggg
     2341 ggccggatgc accagaagct ggatgatgag gttgtttttt gtatgtggtg ctgatttttt
     2401 gtgcgctgtc gggcttcaga cgtgcggaag cctccggcgc aggccggatt attcagagga
     2461 ggtcactttc agggggaagc tgtggccagc cggctgtaat tacggttacg tgacagaatc
     2521 atgcgctcct tcacacgacg ctcaacttcg cgttttaccg cctcacgatt ggcagtgaag
     2581 cgcccttccg cgatttcgcg cgtcagctgc cgtttcacca gggtgacaat atcctgacgt
     2641 tccctgtccg catcacgacg cgcacgggca cgctttattc cccgggactt aagctctgtc
     2701 tgataactgc gaaaacgctc acgaacaaaa cgccaggctt tcgctatcag ttcatccatg
     2761 cccagggtat ccagcccctg ctttttgcgt tgtttgtttt cccataccac acggctgcgg
     2821 cgcgcggcgg ccactgcctc ctctgataca tcgagggcag caaacagtgc agatgtgaac
     2881 gtgatatcgg tcggaatgta gcacccgata agcgggtcat attccgtctg gtaggtaatc
     2941 agtcccagct ctgacaggaa cgtcagggcc cgggtggcac gggtgatgga gagttttccg
     3001 gcagcagact ccgtcgccag tccgcactca atggccagcg tggtgatgga gcactggacg
     3061 cggttggcca gcgggtcata gtggaaacac agcccctgca ggagcgcatc aatagcccgt
     3121 cgacgcagca ctggtggcat gcgtcgacgc agaccacgcg aacgggcatg cgccacatga
     3181 atggcgaaat caaaacggga agtgaagccc accgcctttt ccatcagttt ttcgcagaac
     3241 ttcagcgttc ctgcaccttc acggggtgta aacaccggat tcgggttctt tacctggcgg
     3301 taatacgttt ggtgaagatc agtcacacca tcctgcactt acaatgcgca gaaggagcga
     3361 gcacagaaag aagtcttgaa cttttccggg catataacta tactccccgc atagctgaat
     3421 tgttggctat acggtttaag tgggccccgg taatcttttc gtactcgcca aagttgaaga
     3481 agattatcgg ggtttttgct tttctggctc ctgtaaatcc acatcagaac cagttccttg
     3541 ccaccttacg gcgtggccag ccacaaaatt ccttaaacga tcagtaatct agcactaatc
     3601 ttctgaacac tcaagaatgt aagcccatca tcacatgcat cgtttttgcg cttcactttt
     3661 tatcagtgcg gtcagaactt cagcctgagt cagaccatct tcatgacaca tttgcatgag
     3721 catggcctta tactttggtt caagaaatac ttttacttcc ttgaacgaag ctcttttacg
     3781 ggccactgat aatctttgtt tctctgcatc agaaagcgga ttcccctttc tgtatgctcg
     3841 ttttgcgcca gatgaggaag tcactgcatt ttctgtctgc gacatctcgc ctcctcaata
     3901 ctcaaacagg gatcgtttcg cagaggatac tacagttttt tgaaatcagc gacttgagaa
     3961 ttgtgacgaa gatctgtgtt ggcgcacaaa tcaacgggga ttactgtcgt ttaatgtgat
     4021 ttaaactgtg aaatagtatg gttttcagtt attgaaacgc cgggagcggg gaaaacttgc
     4081 tttttcccgt ttccggggtt ggacaactga gcaacgcgaa ggcgtcagct acgatgttcc
     4141 ggggactgct gatcagcccc tgttgtattc agccattctc cggtcatctg cagcagtttt
     4201 ctggcatcgt ggcttctgag caacggaata ccagcctgcc tcagaacctc ttccagaaga
     4261 atatcccggc gtctgcgttc cggtcgtaaa tggctggcat catccagctc tactgctgca
     4321 acaatactga acgaacgccg ctcaacgata accacatcaa catgccactg agacaccatc
     4381 ctgaataact gccaccactg gcgtgatcgt ggccggatat tcccgttcag ctggacgata
     4441 tccgcgaccc gtacctgcgg acacagatac caccgcttca tatccacaat tctgaacagt
     4501 cctttcataa aactgacttc cctttctgtc agcagcgtct tctgacgttg ccagtattca
     4561 ccgccggaca gtattcgctc tgcatcaccg gtccgtatac ctgccgcatg caattgcatc
     4621 agcaaaggag aatgctgatt tacacttctg aggataatcc ggataagtaa cgctgcaata
     4681 atcagaacaa taagaataaa aataatcagc ttcattgttt atctgctcct ggtaattaat
     4741 tatgctgaat tattacctta catatttcca tatctctttt ggtatcctgt catataactt
     4801 tccggtgatt aattccgcta accggtggtc tgctgctgaa ttaaatgatt cacgttcact
     4861 gtttaataaa ctgtctctgt ttttatgaat aattttttcc agcgtattga ctgatgaaca
     4921 tcgccgtaac tgatacagcc attcctgttt tgttttcgcc atttttattc ccgctgagaa
     4981 cagatgaatg ataagattgc catcgcttta tttacggtgt ctccatatcc aggggggaac
     5041 cggatcagca tctgaccaga gcccacgttt ttgttgtctg gcttcgttct gaaggacagg
     5101 cagcacggga tctgtgttgt actgctcata tacccaggca gccccggccc ggaccataaa
     5161 ctgattaaca ttcattccgt ctggtgcata aacctgcccg agaatacgac cataacgatc
     5221 tcgctgaaaa tatgtgacgg taaccgtttt tcctgcaacc agtgatttca tcatgtccgt
     5281 tgaccaccgg ccataatcct gtttcttttc cggtgcatca atatttatca gcctgatccg
     5341 cactgccttc cgtgagtcca tgacctcgat ggtatcaccg tccagtacac gaacaacccg
     5401 tccatgaata tctgcacaca ataccggcca tgaaaagata aataatacca gtggaatata
     5461 tttcctcatg ttattctcca tatcagataa atggcacctg tatttcaggt aatcctgaat
     5521 atcgcagcac acactgaata acaccttatt ttgtgaaatt aatcacaaaa tataaaatca
     5581 cgacttaata cggaaaaaac tctgtgctac tcagaactgt ccggtcatac ttcccgaccg
     5641 gatattaaaa aaagcaaatc tcatgcatta ttcagccttc tgaatccctg gagtaatgaa
     5701 ggctgctttt tcgttacacc cttccccggc agcgccggtg gcagtgttca gccctttttc
     5761 gggcaaactg ctgccagtct gtccggggag cagactgcgc agcccgtagc catgcctcag
     5821 cggctcccgg ccagtccccc ctgacttcca gccggacagc ccggcgtccg acctgccggt
     5881 agcgccatac cccggagttt tgcgaatcac gccctgttcc gttcattttc cttccccctt
     5941 taatccaggc cggtgaatgt gttataaatt tttataacct tcttctgccg ggtatggaag
     6001 aaggtttcag agaagcctta tttttccgcc tcctttagtg tgaaggaggc ttttttattg
     6061 ttcatcaagc acggcctgaa gttctgcctt tatccggttc tggcggcgga ttttatccag
     6121 acgctctgcc gcatacactt cttcctcctg agaaatatgc tccgtcacat acccttccgt
     6181 gtcataccgg caggcaccgg ctttcatggc acacagatag ctttctgaac gggtgatggc
     6241 cttcagcgcc ctgcgcagtt ttttatgcga gagcgggata ttccgctgcg ccacgtcttc
     6301 cagtaacacg tcccggatac cgcaggccag aagccggggt gtgtcaccgt caaataatcc
     6361 cggccaccag ggtttcaggg tgttcacggc ttcatccagt gtgggcaggt tcagataaat
     6421 ggacagcgcc tgtctggcct gcgctttttt tgctgtcagc tctgcttccc gggcagcctt
     6481 ctccgccagt ttctgctttt tcaccttcca ttttggtggc gtggtgacat tgatgatggt
     6541 tttccggctc cgggtcggtg tttccccttc cgttttccgt ttcagtgtca gtaccggtcg
     6601 tttctgctct gtcatgattg ccctgacctt tgtgaaaata tgtcctccag gtcgttgaaa
     6661 ctactcacta tgatattaag ctgacctcaa cgacctctga catattaata ccgacaatta
     6721 aagatgtttt ttaaaccatt gcgtctgtac tgccgccaca cgttcaaaaa acgcgccttc
     6781 ataaatatca taatggcatg cgtcagcttc ctcgtataat tccttagtac cagaagcaac
     6841 agcatcatat aaggcttttc cctgttctgg tggattaaca ctatcctggc cggcaataac
     6901 aatcaaaact gggcactgta ctttggctgc gctctctgcc ggtttatatt gcaacgtctc
     6961 cataaccgta aggaaaggta tttttatatc catttcagga tactgacctt ttaccttctc
     7021 gaagaatacc tttgattcat tatcactcag tacccgggta acccccacaa acatctcttt
     7081 cccggtgttt ttcttcttct ctgccatttt attcagcgtg gacagaaaag acgctctctc
     7141 cgattcattc atctctccgg taaccagaac atcaccgtca gcaaaagcca gctggctgac
     7201 aatacatttt acgcgttggt cctgcgcagc tgcactgaag acatgccccc ctcccagcga
     7261 tgttccccat aacccaatac gctgattatc aatacattcc tgcttttctg cccaattgat
     7321 aactgaaata atatcttcgg tctgcattgc aggaaccaga cgccctcgtt ctccatcact
     7381 ctcaccgaat cctcgataat caaaagtaat cgttgcgaat cctgcttctg tgaaggcatt
     7441 cgcaaagcaa gggagcagaa cattcctgat accgcaaaac ccatggcata agataatcaa
     7501 aggatgtttt atatttcctt caggaacacg aaatgtcagg gcaatacctt cagagagttt
     7561 gtgatccgta attttcataa atatcctgtg ttatgttgtt tattaataat cagaatcagc
     7621 gtcagtatca taccagtcac aacgccagaa cgcccagcac agccagatga caggcgtaga
     7681 ataccgggaa aaaatccccg ggccagaaac ggggtaatga ttttcctgca cacgacacca
     7741 gaccaacggt cagcaccgtc attacaagac cggccaccgc cgctgcatca ctggaggcaa
     7801 ggttaagtgc cggaataacg gccagcaggc atgcaagcag cgccagacgt tccgctctgt
     7861 cttccgcccg gtacagtcgg taactgaccg ccagcatcag cagcccggca atgccgtaac
     7921 tggtgccgga caaaggcccc cacagggcca tcagaagaat ggccgctgct gtacgccacc
     7981 cactgcgcgt ttcacaccac gtcagcacct gtgccgccac tgcaaaggca aacaggatat
     8041 tcccctcata ccagggaaag ccggccagat aatatgcgaa ctgggcaata attccccatc
     8101 cccacagccg gttaatggcc ggttgccgga tatgcgcatg acgggacaga ttcagccccc
     8161 acaccagggc aaacagcgga aaggctcctc gcccggccag aaacatccat tcctgcttca
     8221 gctggaatat cagattgatg tggtccagta ccatcagcac cagcgccacc gttttgatga
     8281 tatcccgctg tccgggtgac cagaccagaa acgactgcaa ccaggtatcg gtccgtgcag
     8341 ccagtgaatc gttacgtgtc gtgttcgtgt tatccgttgt catcagcgtt tatcttcctg
     8401 tatcagtctc cgcccagggt tttctctttc tgcaggtcac gaaccatatc ccgctctatc
     8461 tgctgcagac gctcctgcag cagattttcc cgggcaacct cccggaccgc ttcctgctcc
     8521 cgttgtgatt cacgcagcac actctccggc agcgcggctt cccgttcagt tatgtcagcc
     8581 cggtcacgct cctgcccggc aatttccctg acagcctgct ctgttttgcc atccggcagg
     8641 tcaggtttgt tctctgccat tttacggaca atttcatctg cacggcgttc cgtttcacgg
     8701 cggatggcct cttcagcctg ccgctgtgcc agcacctctg ccgtgaccgg ttcgccattt
     8761 ccggctcccg gctggacgct gctgtccggg atatcccccc acacgcgacc accggtgatg
     8821 gcaccgggat tccacggacg accttcaccg gcaattctta ccaccacacc gctgtcagga
     8881 ttatcacggg caatccggac accatcctgc atattatcag ccagcaggct ctctccgtta
     8941 cggctaccct gcagggccac gaactgcgca tccccgctgc ctttcacccg tccttcaccg
     9001 ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagtg ggttcagcca gataccggcg
     9061 gacttgccgt tacggtcaaa cgccggcagt gccacatacg gctgcggata tttacgtccc
     9121 ggtgcaataa accgtgcagg gctgtctccc ccggccagcc ctgcctgacg gagaacggca
     9181 cggcctgccg ccacgtcccg cagttcccgt gccgtactga acagccgctg cgcattcatg
     9241 acctcccggt ccggtttcgg ctccagcaca tcgtgggcag ttcctttctg tacggcattg
     9301 ttaatggcat ccgtccagcc ctgacggtta tcggtgtaca cctgcacatg ctgcttcata
     9361 cgcgacaggg ccacgtaggc tgactcaaag ccggccatca gtttccggtt accttccgtg
     9421 ccttcaagcg cgatggcaaa ggtttcactc gccccctgcg caccgtgggc ggtgatggca
     9481 taagccaggt caatatgttg ctctgcccgc tcctggccgg ggcgaatcac ccgggtctgc
     9541 tgtccgtctg acaacgtgac actgtcaccg gaaaccgctg tcaccgtcca gacgctgttg
     9601 gccacataac cgcgctcccg gtcactcttc gtgaagcgca tccggtcgcc ggtccccacc
     9661 cggattttgt ccggggtgta cagtgtgaca ccttcagcca ccgcctcccg gggcgaaatc
     9721 agccgcgtgt taccttccgc atcctccagg gttatcagcc cgtcatcctt gctgataccg
     9781 gcaatccggt gatacacact atccaccagg gcaagggcat ccgggttatt ctcccaggtg
     9841 gagagacgac gcagctcccc gtcacgtata ttcgctgtgt tcaggacagg caccatgacc
     9901 tgctctttcc ccagctcacc ggccttttcc cgtgcatcat gaatcatgct gttcagtacg
     9961 cgccggtcct cattcaggtg cgtgacaatc agcgtctgct cccgtgcttc cggtgtcctg
    10021 ccggtatagt cgcggacaat ggcttcatac agtgtcatgg ggacatccgg aaaagtctca
    10081 cctttcagca tcgccttctg ctgcgcttct gccagtttcg cttcctggct gtgactgaac
    10141 tccgtcacgg agtgctccgg tgcccatgcg ccctcctgac ggggaacctg agacggtttc
    10201 acactctcaa gcccggacag tgccctttcc acatcccggt taatcaggct gtataccgcc
    10261 tcccgcagtt ccggcgtctg acgcacaatc tccttcatga tgaccacatc ggcagcactg
    10321 cgcgtctgct ggagacggaa aggctgaccg ggcgcgatgg cctgcagctg gtccgtgtca
    10381 ccactggcca cggcacgacc gccaccggcc gcaatcaggg cgtatgcccg tgccatgtcg
    10441 gtattgccca ccattgagct ctcatcgagc aggaacagcg tgttgctgaa atccggcgtt
    10501 tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg acgccagcgt ctgtgcatcc
    10561 acgccggcgc tgcgcatctc accgaccgca cggtgcgtgg gccccagccc cacaactcgg
    10621 ggacgctcac tcgccggcag catgttcacg gctgacatca ccgcccggaa ctgtgtggtc
    10681 ttacccacac cggcataacc ctgtaccacc gtgaaacggt cggacgtttc cagtatcata
    10741 cgggtggcgg cacgctgtcc cgacgttaac gtctccatga gttcgccggg tactctctcc
    10801 atcagcggcg tgaccgcctc cttaccttcg agaatatggc gaagaatact tttttctgcc
    10861 tcatacgacg cacgggaaac cagcaggcct gtgccatagc cttttgccac atccacatac
    10921 agtaagtcac cgcgttttat ctgcgcattg atttcccctc ccagttcagt aaaaccggtt
    10981 ccttctgcag caaacgactt cgcctctgtc agcaggtcca ccatgctgaa ggccaggttt
    11041 ttactttcca gcacaggaag ggccagatga atggcctgct gtgccggcgt gtgcagcccg
    11101 gttttctgca gactgatagc ggtttccagc gatgtctcac cggcccgcgc ctttatctgc
    11161 tcagaaacca ccgtaaagga gggatggcgg gcaagttttt ccgcagtacg ggtttcatcc
    11221 agtgaggaat acagccggac atcacgaccg ctgcgggcca gaccgttcag ggtggcgttg
    11281 tccattgcca tctgtgtgac ggaggcaaac accgtcgcac tgtcactgac ggaatgcccg
    11341 ggcgtttcca cccagccgtt ctccagcttc agtgccgtga acggtgaatc agccacaggc
    11401 agggtggccg gttcagcccg ccccggcaca acaaccgtca tcgcatcttc actgacggat
    11461 gccacctgca ggcggtcacc gccggagacg cggagtccgg gaattttccc tgtcaccctc
    11521 agtcgctcgc cgtctgccac cggcattttt tccggccgga acagcgacca gctgctgtcc
    11581 agggaggaaa tacgcaccac ctgcgtttcg ccctgcgcat cccgcagggt caggctgtga
    11641 ctctgcgccg tcacccggtc gataacatag cggtcatgac tgcgtgtctc cgggttccac
    11701 tgctccatca ccatccccgg tcggtacata tcccgcagat aacggctccg gctgtccagc
    11761 cagaccggtg acagggcggt catggtcacc tccgggtgtc cgagcacgcc ctgtgttttc
    11821 agctcactgc gaatggcctg tgtcagtatg gcctgttccc gtaccccgct gacctgtgcc
    11881 acgctctctt ctccggcttt cacgctggcc gcaaaatctc cggccagccg gtcatagcgg
    11941 acattccggt ccggttcact gatgatggtt gccggtcgct gttctccgcc ctgccagcga
    12001 tacgtgttca ccccggcatc cttcatggcc atcagcgcac tgcctgtacc ggttcgctgc
    12061 ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg caccatccag cagggttaac
    12121 gtctctttca gggagagttt ttcgccctgg tcgacgataa cagtactgcc cggcgtgaag
    12181 accatgccct cctgcagctg acgacgtccg gttatcagct caccggacag ccgttcatcc
    12241 tgcttcaggt tcatctgcga gcgacggtca gcagcgataa tctgcacctc ccgcccctgc
    12301 tcccgggcca tcatgaccag ttcagccacc cgctcacgct gcccggctgc accgccctgc
    12361 ccggacacaa tggccagcga cggacggtcc tgtgccagaa cactgaccgc atcgctgtaa
    12421 ccagccgtcc gggggacact tttctccgga tgtacggtca cccggttctg tttcatgatg
    12481 tcacgactga gtgcccggac ggacagctca tcgagcacat gaattcctga tgtgaacagc
    12541 cccttctcac ggtcgagggg gataagctgc tcacggctga tggcctcatc gataccggcg
    12601 cgtgcccgtt caatcacacc attttccggc ggcagtatgc cgaccgtcct ggccagcacg
    12661 tccgtatacg tgaactgcac ttttcgttca cttaatccgg caatcgcctg tgtcaccgcc
    12721 tgctgcacat ccggcccgtc ctgtgaagca ggcccgggcg cctgcgtgcg gatctccgca
    12781 cgctgatccg ccgcgtcacg atatgcccgg atgtcgaacc cggtttcctt cagcgtctgc
    12841 atccattcag ccattctgac ttcaggatcg acgtgctgtt tggattttcg cgtatccagt
    12901 gccgccacat cccgggattt cagcgaggcg tcttcaccga cggcctccct gatagcctgt
    12961 gaacgactgg aaaaggcctc caccggtacg cccggcatct cccacatacc gtgcttaccg
    13021 accacttcgg tttcataacc cagcgcctca acgcgctgac gtaactcact ctgatatatt
    13081 ttcccgaacg caatccggtt cgcgagcacg ttctcggaaa atccggtttt ccccactttg
    13141 tcactgctca gtgtcttcca ttcgccgtta tgctgcgtga cattagccac caccacatgc
    13201 gtgtgtaact gtgggtcctg atcgcgactg gtgtcatggt taaacagtgc catcaccaga
    13261 ttaccggtca gcaccgtttc tgactgtccg tccgtcatca cccgtgtgga agccagcgcc
    13321 tccacctgac ggacagcaaa atccacagcc tggttatgtg catcaataag acgtttatcc
    13381 cccccgagca tggccatcat ggagacactt ttgggggcgg agaaggtcag gtcgtagccg
    13441 ggacgatgct tattactgcc atcctgcatg cggcttaaat ccgccccgtc cggcagcctg
    13501 ccctccagaa gacgggtaaa aacatcctta tcgacgctgc cctgcagccc aagctgttca
    13561 gccccctgac cggcccagcg ttctcccatg ctgcccagca catagtaatt atccttgtcg
    13621 gtgtaatagt ttccggcact gcctgccgat ttaacaacgc tgaaactcaa catcagaaat
    13681 catctcccgg ctcaacatcc tccccgcgct cccggtgcac attaatgttc acctcttcac
    13741 gacgctgcat atgctgctgg atgtccggat gattttcctg ttgccatgcc tcataagcgg
    13801 ccatatccac cacttcaccg gattcactga tcccgggtgg cagttgctgt tccatctctt
    13861 cttccggttt cattttcagc tcctgctcga tcccccctgc cggaacactc acacctgcat
    13921 ctgacttctt atcattgatg acagaagaca
//
LOCUS       DBBBSB010000103        13873 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.103, whole genome shotgun sequence.
ACCESSION   DBBBSB010000103 DBBBSB010000000
VERSION     DBBBSB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.103"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACIUS0_003098"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="ACIUS0_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGB0220748.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /locus_tag="ACIUS0_003099"
     CDS             323..1954
                     /locus_tag="ACIUS0_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGB0220749.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="ACIUS0_003100"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="ACIUS0_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGB0220750.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="ACIUS0_003101"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="ACIUS0_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGB0220751.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="ACIUS0_003102"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="ACIUS0_003102"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGB0220752.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="ACIUS0_003103"
     CDS             6428..7057
                     /locus_tag="ACIUS0_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGB0220753.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACIUS0_003104"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="ACIUS0_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGB0220754.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACIUS0_003105"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="ACIUS0_003105"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGB0220755.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="ACIUS0_003106"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="ACIUS0_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGB0220756.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="ACIUS0_003107"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="ACIUS0_003107"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGB0220757.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3619 a         3599 c         3362 g         3293 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttt
//
LOCUS       DBBBSB010000104        13248 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.104, whole genome shotgun sequence.
ACCESSION   DBBBSB010000104 DBBBSB010000000
VERSION     DBBBSB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.104"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACIUS0_003108"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACIUS0_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACIUS0_003109"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACIUS0_003109"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGB0220758.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2163..3197
                     /gene="rhaT"
                     /locus_tag="ACIUS0_003110"
                     /pseudo
     CDS             2163..3197
                     /gene="rhaT"
                     /locus_tag="ACIUS0_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACIUS0_003111"
     CDS             complement(3194..4042)
                     /gene="rhaR"
                     /locus_tag="ACIUS0_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGB0220759.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACIUS0_003112"
     CDS             complement(4116..4952)
                     /gene="rhaS"
                     /locus_tag="ACIUS0_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGB0220760.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5240..6709
                     /gene="rhaB"
                     /locus_tag="ACIUS0_003113"
     CDS             5240..6709
                     /gene="rhaB"
                     /locus_tag="ACIUS0_003113"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGB0220761.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6706..7965
                     /gene="rhaA"
                     /locus_tag="ACIUS0_003114"
     CDS             6706..7965
                     /gene="rhaA"
                     /locus_tag="ACIUS0_003114"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGB0220762.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8270..9031
                     /gene="rhaD"
                     /locus_tag="ACIUS0_003115"
     CDS             8270..9031
                     /gene="rhaD"
                     /locus_tag="ACIUS0_003115"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGB0220763.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            9041..9355
                     /gene="rhaM"
                     /locus_tag="ACIUS0_003116"
     CDS             9041..9355
                     /gene="rhaM"
                     /locus_tag="ACIUS0_003116"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGB0220764.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9656..10102
                     /locus_tag="ACIUS0_003117"
     CDS             9656..10102
                     /locus_tag="ACIUS0_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGB0220765.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10113..11564
                     /locus_tag="ACIUS0_003118"
     CDS             10113..11564
                     /locus_tag="ACIUS0_003118"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGB0220766.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11554..12624
                     /locus_tag="ACIUS0_003119"
     CDS             11554..12624
                     /locus_tag="ACIUS0_003119"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGB0220767.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12624..>13248
                     /locus_tag="ACIUS0_003120"
     CDS             12624..>13248
                     /locus_tag="ACIUS0_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HGB0220768.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3158 a         3458 c         3491 g         3141 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat caatgccgta
     1981 cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact
     2041 taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag
     2101 cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga
     2161 agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag
     2221 cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag
     2281 tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg
     2341 atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg
     2401 gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt
     2461 cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa
     2521 ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg
     2581 gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc
     2641 gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga
     2701 tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg
     2761 aagccgctgc cgcacttggc gtcgatccac tgtatgttgc tctgccaagc tatgttgtca
     2821 tcatgggcgg cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga
     2881 aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt
     2941 tactctcggc actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc
     3001 acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg
     3061 tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc
     3121 cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca
     3181 tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc
     3241 cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg
     3301 aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga
     3361 cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa
     3421 cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc
     3481 tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg
     3541 aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag
     3601 ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct
     3661 gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc
     3721 taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa
     3781 taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa
     3841 tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat
     3901 taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt
     3961 gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga
     4021 gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg
     4081 caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc
     4141 tggcgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta
     4201 ctgtcgctga atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt
     4261 agatgtcggg ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt
     4321 tgctgcttaa gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca
     4381 tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt
     4441 gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc
     4501 ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc
     4561 atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac
     4621 ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat
     4681 cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga
     4741 tgccgatcat gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca
     4801 ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga
     4861 tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac
     4921 ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc
     4981 gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa
     5041 aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac
     5101 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     5161 ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga
     5221 aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc
     5281 cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga
     5341 aatccatcgt tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga
     5401 tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat
     5461 cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca
     5521 gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc
     5581 acaacagcaa ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt
     5641 caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat
     5701 tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg
     5761 ggaatatacc aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga
     5821 gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg
     5881 taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc
     5941 cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta
     6001 tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga
     6061 cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct
     6121 gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga
     6181 tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc
     6241 caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg
     6301 ggaaacggcg caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag
     6361 tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt
     6421 ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc
     6481 cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat
     6541 cggcatcgag ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt
     6601 cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta
     6661 tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg
     6721 gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag
     6781 gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt
     6841 tccggttttg aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat
     6901 ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg
     6961 attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc
     7021 tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat
     7081 cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt
     7141 acgctctcgc atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc
     7201 cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg
     7261 atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg
     7321 gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt
     7381 gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac
     7441 atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg
     7501 actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg
     7561 cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc
     7621 caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt
     7681 gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg
     7741 aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg
     7801 gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgcggcag
     7861 gcggtctggg aaatgtattg ccaacatcac gatacgccag caggtagcga atggctggag
     7921 agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg
     7981 gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc
     8041 ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa
     8101 caccgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc
     8161 cgcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac
     8221 ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg
     8281 ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta
     8341 tcacgacaac ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact
     8401 ggcaaatacg ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga
     8461 tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct
     8521 ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca
     8581 ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac
     8641 caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact
     8701 gtgggaaggc agcaccgaat gtctggtagt attcccggat ggcgttggca ttctgccgtg
     8761 gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc
     8821 gctggtgttg tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt
     8881 tggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg
     8941 cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt
     9001 tacaccacta gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt
     9061 catgcaggta aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc
     9121 ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa
     9181 agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt
     9241 agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa
     9301 cccagataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc
     9361 caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat
     9421 attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat
     9481 gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc
     9541 aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc
     9601 acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc
     9661 agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
     9721 gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct
     9781 gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgcagt
     9841 gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc gcaaagcgca
     9901 ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
     9961 gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat
    10021 tcaccaggaa tttattcatc aactgcaaca gggcgatacc gaccaggtgc tggccttgtt
    10081 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    10141 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    10201 aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa
    10261 accgcttatc cagcgaagag attgccgctg ccgattacgt cattcttgct accgggcgtg
    10321 gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct
    10381 ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt
    10441 ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga
    10501 gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg
    10561 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    10621 cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc
    10681 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    10741 ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat
    10801 tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag
    10861 tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc
    10921 tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg
    10981 gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc
    11041 tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct
    11101 tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg
    11161 cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc
    11221 gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac
    11281 cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg
    11341 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg
    11401 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg
    11461 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc
    11521 gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg
    11581 tgcgaagcca gcgccgttag cggcgatgaa caggaagttc gcgacatcct gataaacacg
    11641 ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    11701 ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc
    11761 acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg gtggaatcag
    11821 tcgatgctca accatcgggt aaccatacgc acacacaagg gagtgaaaat ccctggtgtg
    11881 attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt
    11941 gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg
    12001 gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    12061 gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg
    12121 gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta
    12181 cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt gctggatacc
    12241 gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    12301 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtagcagcg
    12361 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccgga
    12421 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc
    12481 ctgccaaccc gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct
    12541 ctgctcacgt tgatacgggg ttttctgacg accttaaccg cggagaaagt caacgcgttt
    12601 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    12661 tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga
    12721 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg
    12781 ccagtggcag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    12841 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat actttcactc
    12901 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc
    12961 cccagttaaa acagcgttat gaacgcactt gttgcctggc cagccgccct ggtttgggcc
    13021 atttcattga tgagacagaa gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag
    13081 atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat ttatccacgg
    13141 cctgcgacaa ccaacaccgc tggccgctca tacagggtga ttatctctcc agcctgattc
    13201 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DBBBSB010000105        13098 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.105, whole genome shotgun sequence.
ACCESSION   DBBBSB010000105 DBBBSB010000000
VERSION     DBBBSB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.105"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACIUS0_003121"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACIUS0_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGB0220769.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACIUS0_003122"
     CDS             1131..1931
                     /locus_tag="ACIUS0_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGB0220770.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACIUS0_003123"
     CDS             2009..2779
                     /locus_tag="ACIUS0_003123"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGB0220771.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SMPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACIUS0_003124"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACIUS0_003124"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGB0220772.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACIUS0_003125"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="ACIUS0_003125"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGB0220773.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="ACIUS0_003126"
     CDS             5034..5756
                     /locus_tag="ACIUS0_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGB0220774.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACIUS0_003127"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="ACIUS0_003127"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGB0220775.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACIUS0_003128"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="ACIUS0_003128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGB0220776.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="ACIUS0_003129"
     tRNA            7149..7225
                     /locus_tag="ACIUS0_003129"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="ACIUS0_003130"
     CDS             complement(7356..8402)
                     /locus_tag="ACIUS0_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HGB0220777.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACIUS0_003131"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="ACIUS0_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACIUS0_003132"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="ACIUS0_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGB0220778.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACIUS0_003133"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="ACIUS0_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGB0220779.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="ACIUS0_003134"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="ACIUS0_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACIUS0_003135"
                     /pseudo
     CDS             complement(12179..13093)
                     /gene="tagK"
                     /locus_tag="ACIUS0_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3008 a         3392 c         3484 g         3214 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtatg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttggttt cattgtgt
//
LOCUS       DBBBSB010000106        13017 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.106, whole genome shotgun sequence.
ACCESSION   DBBBSB010000106 DBBBSB010000000
VERSION     DBBBSB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.106"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..319)
                     /locus_tag="ACIUS0_003136"
     CDS             complement(<1..319)
                     /locus_tag="ACIUS0_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HGB0220780.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="ACIUS0_003137"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="ACIUS0_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACIUS0_003138"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACIUS0_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGB0220781.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACIUS0_003139"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACIUS0_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HGB0220782.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="ACIUS0_003140"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="ACIUS0_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGB0220783.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="ACIUS0_003141"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="ACIUS0_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HGB0220784.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVHHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="ACIUS0_003142"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="ACIUS0_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGB0220785.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="ACIUS0_003143"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="ACIUS0_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="ACIUS0_003144"
     CDS             10613..11503
                     /locus_tag="ACIUS0_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGB0220786.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="ACIUS0_003145"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="ACIUS0_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGB0220787.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="ACIUS0_003146"
     tRNA            complement(12493..12569)
                     /locus_tag="ACIUS0_003146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13017
                     /locus_tag="ACIUS0_003147"
     CDS             12812..>13017
                     /locus_tag="ACIUS0_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGB0220788.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3187 a         3377 c         3305 g         3148 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gcatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DBBBSB010000107        12907 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.107, whole genome shotgun sequence.
ACCESSION   DBBBSB010000107 DBBBSB010000000
VERSION     DBBBSB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.107"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACIUS0_003148"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACIUS0_003148"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGB0220789.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACIUS0_003149"
     CDS             complement(1806..3224)
                     /locus_tag="ACIUS0_003149"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGB0220790.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACIUS0_003150"
     CDS             complement(3378..3722)
                     /locus_tag="ACIUS0_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGB0220791.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACIUS0_003151"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACIUS0_003151"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGB0220792.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACIUS0_003152"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACIUS0_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACIUS0_003153"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACIUS0_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGB0220793.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACIUS0_003154"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACIUS0_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGB0220794.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACIUS0_003155"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACIUS0_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGB0220795.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACIUS0_003156"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACIUS0_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGB0220796.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACIUS0_003157"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACIUS0_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGB0220797.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACIUS0_003158"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACIUS0_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGB0220798.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACIUS0_003159"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACIUS0_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGB0220799.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACIUS0_003160"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACIUS0_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACIUS0_003161"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACIUS0_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGB0220800.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACIUS0_003162"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACIUS0_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3400 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DBBBSB010000108        12883 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.108, whole genome shotgun sequence.
ACCESSION   DBBBSB010000108 DBBBSB010000000
VERSION     DBBBSB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12883
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.108"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1173
                     /gene="proV"
                     /locus_tag="ACIUS0_003163"
     CDS             <1..1173
                     /gene="proV"
                     /locus_tag="ACIUS0_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGB0220801.1"
                     /translation="YKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEG
                     EIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVF
                     QSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMR
                     QRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEA
                     MRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNG
                     LIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALI
                     DAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1166..2230
                     /gene="proW"
                     /locus_tag="ACIUS0_003164"
     CDS             1166..2230
                     /gene="proW"
                     /locus_tag="ACIUS0_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGB0220802.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2288..3280
                     /gene="proX"
                     /locus_tag="ACIUS0_003165"
     CDS             2288..3280
                     /gene="proX"
                     /locus_tag="ACIUS0_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGB0220803.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3472..4651
                     /locus_tag="ACIUS0_003166"
                     /pseudo
     CDS             3472..4651
                     /locus_tag="ACIUS0_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4775..5512
                     /gene="ygaZ"
                     /locus_tag="ACIUS0_003167"
     CDS             4775..5512
                     /gene="ygaZ"
                     /locus_tag="ACIUS0_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGB0220804.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5502..5837
                     /gene="ygaH"
                     /locus_tag="ACIUS0_003168"
     CDS             5502..5837
                     /gene="ygaH"
                     /locus_tag="ACIUS0_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGB0220805.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5928..6458
                     /gene="emrR"
                     /locus_tag="ACIUS0_003169"
     CDS             5928..6458
                     /gene="emrR"
                     /locus_tag="ACIUS0_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGB0220806.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6585..7757
                     /gene="emrA"
                     /locus_tag="ACIUS0_003170"
                     /pseudo
     CDS             6585..7757
                     /gene="emrA"
                     /locus_tag="ACIUS0_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            7774..9312
                     /gene="emrB"
                     /locus_tag="ACIUS0_003171"
     CDS             7774..9312
                     /gene="emrB"
                     /locus_tag="ACIUS0_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGB0220807.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9376..9891)
                     /gene="luxS"
                     /locus_tag="ACIUS0_003172"
     CDS             complement(9376..9891)
                     /gene="luxS"
                     /locus_tag="ACIUS0_003172"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGB0220808.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10041..11597)
                     /gene="gshA"
                     /locus_tag="ACIUS0_003173"
     CDS             complement(10041..11597)
                     /gene="gshA"
                     /locus_tag="ACIUS0_003173"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGB0220809.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11670..12098)
                     /locus_tag="ACIUS0_003174"
     CDS             complement(11670..12098)
                     /locus_tag="ACIUS0_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGB0220810.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12095..12661)
                     /gene="yqaB"
                     /locus_tag="ACIUS0_003175"
     CDS             complement(12095..12661)
                     /gene="yqaB"
                     /locus_tag="ACIUS0_003175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGB0220811.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2972 a         3403 c         3316 g         3192 t
ORIGIN      
        1 tataaaatat ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca
       61 aaagaacaaa ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc
      121 attgaagaag gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg
      181 gtacgccttc tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg
      241 gatattgcca aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg
      301 gtcttccagt cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt
      361 atggaattgg ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt
      421 caggtcgggc tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt
      481 caacgtgtgg gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa
      541 gccttctcgg cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta
      601 caggcgaaac atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt
      661 attggcgacc gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat
      721 gaaattctca ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt
      781 agtcaggtgt tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa
      841 actcctggct tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat
      901 ggctacgtta tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa
      961 accgcgttaa cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc
     1021 gatgcacaaa cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg
     1081 cccgtggtcg acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc
     1141 gctttagatc gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc
     1201 agcggcggac agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc
     1261 tgacggcggt ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa
     1321 tattctcgat ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat
     1381 cgactgggtt gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta
     1441 tatcctcaac ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt
     1501 tttcgctctc atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc
     1561 gctgattgcc atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt
     1621 gttaactgcc ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag
     1681 aagtccgcga gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc
     1741 gtttgtttat ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt
     1801 ggtgacgatc atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca
     1861 ggttccggcg gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct
     1921 gttcaaagtt cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct
     1981 gatgctggcc ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca
     2041 gatggtactt cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg
     2101 gattgtgatc ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg
     2161 cagtcgcggc aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt
     2221 cattaagtaa ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg
     2281 aataacaatg cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca
     2341 aacttttgct gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat
     2401 cactgaagaa accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac
     2461 cgtcaacaaa cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga
     2521 tgcaaccttc accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg
     2581 tggcgataag aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct
     2641 gatcgataag aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc
     2701 gaagatcgcc aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa
     2761 ccctggctgg ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa
     2821 caccgtgacg cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta
     2881 caaagagggc aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct
     2941 gaagccaggg aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa
     3001 aaatgccgat accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca
     3061 tatcgttgcc aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat
     3121 tatgcagttg ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc
     3181 ctcagaaggc gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt
     3241 cgatggctgg gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag
     3301 gcgttttcgc ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta
     3361 tcatgcctac aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa
     3421 catccgctat tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa
     3481 cctaatcatg agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg
     3541 gtagccagta actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt
     3601 tccgccagtt cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta
     3661 ctgtttcttg ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc
     3721 ttactggcag cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc
     3781 ctcggtacgg cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca
     3841 gcgacgctgg cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg
     3901 ctgttgggga tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg
     3961 cgcaccgtct tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt
     4021 ggtctgccac aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt
     4081 ttcagcatgt tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc
     4141 tttgccaact tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt
     4201 aactacagcg atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc
     4261 gctcgtccgg cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct
     4321 tctgctgtta ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat
     4381 tatcggcatt ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt
     4441 aatttatcga atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta
     4501 ctttattggc ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg
     4561 ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg
     4621 aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac
     4681 acttataagg gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta
     4741 tttataatat taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc
     4801 tcctggttcg gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta
     4861 tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga
     4921 aagcgttttt ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat
     4981 gctggcagcc gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg
     5041 ccatgttttg tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa
     5101 aaccgcccta tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact
     5161 ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg
     5221 gtcatcgtgg gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg
     5281 ttatcccgcc gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct
     5341 gctcgcctct ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct
     5401 tgcaggcgta acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg
     5461 cctcactgcg ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc
     5521 tgcttgggtt actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc
     5581 gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg
     5641 gcatcgcctc gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata
     5701 cacgccgttt cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa
     5761 cacgcagcat tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag
     5821 tgatggcgat tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt
     5881 gtcactgtcg ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt
     5941 ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc
     6001 aggagatcct tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca
     6061 ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg
     6121 agtctcagga aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc
     6181 gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta
     6241 aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt
     6301 tgcgcgaggt tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca
     6361 caacagaaaa agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga
     6421 tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc
     6481 cagatttata aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag
     6541 caggtcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg
     6601 agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc
     6661 ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg tttttggtac
     6721 tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt
     6781 ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag
     6841 gcgatgttct ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa
     6901 ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc
     6961 aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc
     7021 gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg
     7081 ccgtcaccag tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg
     7141 cgatgattct ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag
     7201 tacgtaacgc ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg
     7261 tttcccgtcg tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg
     7321 tcgttccagc caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata
     7381 tgcgcatcgg tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca
     7441 ccggtaaagt ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc
     7501 aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg
     7561 accagaaaca gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca
     7621 ataccactaa ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg
     7681 taagtaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa
     7741 aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc
     7801 gcgcaactgg tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac
     7861 tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc
     7921 caaggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc
     7981 ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt
     8041 gctattgcgt cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc
     8101 gtgattcagg ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat
     8161 aactacccgc cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc
     8221 gcgccaattt gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg
     8281 atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg
     8341 cgcggacgtg aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg
     8401 gttattggta tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt
     8461 tcatcacagg aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt
     8521 gtctgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac
     8581 ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt
     8641 ctgctgccgc agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc
     8701 tctgcgccgg tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat
     8761 aaactggata tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac
     8821 tggcgtgcct atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt
     8881 atccaggggt ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt
     8941 ttgccaccgg aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg
     9001 gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat
     9061 gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa
     9121 ctggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc
     9181 aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc
     9241 gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc
     9301 ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg
     9361 aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc
     9421 ttcgttgctg ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc
     9481 ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt
     9541 ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg
     9601 ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat
     9661 atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg
     9721 ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag
     9781 atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc
     9841 aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc
     9901 tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg
     9961 aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg
    10021 agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta
    10081 tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc
    10141 tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac
    10201 gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa
    10261 gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg
    10321 ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta
    10381 cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt
    10441 ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg
    10501 ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc
    10561 tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata
    10621 tattcaatgc cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt
    10681 tttggacgaa tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc
    10741 agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt
    10801 gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt
    10861 tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa
    10921 tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa
    10981 gaacaaatcg ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa
    11041 ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata
    11101 tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa
    11161 atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag
    11221 cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag
    11281 catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc
    11341 agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc
    11401 agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct
    11461 tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc
    11521 agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt
    11581 gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg
    11641 taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc
    11701 gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc
    11761 ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc
    11821 cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc
    11881 cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca
    11941 aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc
    12001 aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta
    12061 ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt
    12121 ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact
    12181 gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat
    12241 ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg
    12301 ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac
    12361 catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac
    12421 ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca
    12481 gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct
    12541 gaatatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag
    12601 gcgccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca
    12661 tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg
    12721 ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat
    12781 atttttgaag gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc
    12841 gctcggttcg tagccgagta ctctatccag ctgagctacg gat
//
LOCUS       DBBBSB010000109        12734 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.109, whole genome shotgun sequence.
ACCESSION   DBBBSB010000109 DBBBSB010000000
VERSION     DBBBSB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.109"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1038
                     /locus_tag="ACIUS0_003176"
     CDS             <1..1038
                     /locus_tag="ACIUS0_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGB0220812.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1039..3204
                     /locus_tag="ACIUS0_003177"
     CDS             1039..3204
                     /locus_tag="ACIUS0_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGB0220813.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3218..4129
                     /locus_tag="ACIUS0_003178"
     CDS             3218..4129
                     /locus_tag="ACIUS0_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HGB0220814.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4129..5424
                     /locus_tag="ACIUS0_003179"
     CDS             4129..5424
                     /locus_tag="ACIUS0_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HGB0220815.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5469..5699
                     /locus_tag="ACIUS0_003180"
     CDS             5469..5699
                     /locus_tag="ACIUS0_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220816.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5677..6177
                     /locus_tag="ACIUS0_003181"
     CDS             5677..6177
                     /locus_tag="ACIUS0_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HGB0220817.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6177..7595
                     /locus_tag="ACIUS0_003182"
     CDS             6177..7595
                     /locus_tag="ACIUS0_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HGB0220818.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7595..8443
                     /locus_tag="ACIUS0_003183"
     CDS             7595..8443
                     /locus_tag="ACIUS0_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HGB0220819.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8443..8898
                     /locus_tag="ACIUS0_003184"
     CDS             8443..8898
                     /locus_tag="ACIUS0_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGB0220820.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8901..9593
                     /locus_tag="ACIUS0_003185"
     CDS             8901..9593
                     /locus_tag="ACIUS0_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGB0220821.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9603..10934
                     /locus_tag="ACIUS0_003186"
     CDS             9603..10934
                     /locus_tag="ACIUS0_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGB0220822.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKSNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10935..12665
                     /locus_tag="ACIUS0_003187"
     CDS             10935..12665
                     /locus_tag="ACIUS0_003187"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGB0220823.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3438 a         3143 c         3355 g         2798 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgtaagttt
     1081 gacgcagact ggatggcgag cgatgaagcc agaaccgagg cgacaaatga cctgtatttt
     1141 agccgagtgt cgcaatggga tgactggcta tcaaactaca ctaccctgca atatcgcgga
     1201 caattcgatg ttgttcgccc ggaggtcagg aaactggtcg cagagatgcg ccggaaccct
     1261 gtcgacgttc tcttccgacc caaagacggc gctaatcctg atgcagccga tgtattgatg
     1321 gggatgtatc gtactgatat gcgccataac acggcaaaga ttgccgttaa cgttggcgtt
     1381 cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg tcacgcagta cgaagacaac
     1441 gatccaacaa gcaacaatca ggtaatccga cgcctgccaa tccatgaagc ctgctcacac
     1501 gtcatatggg acgccaacag caagcagatg gataagagcg acgctaagca ctgcacggtg
     1561 attaacgcct tgtcgcgcaa tggctggaaa gagttcgcag aggattacgg tattgacccg
     1621 gataccctgc catctttcca gaatcctaac gatacatggc tattcccgtg ggtgtcgaat
     1681 gatgtcgtct acgtcgctga gtattacgag gtagaagagg agaaggagaa ggtcttcatc
     1741 taccgcgacc cgctgacagg tgagccggtc agctattacc agcaggatat caaagacgtc
     1801 atcgacgacc tggctaatcg tggattcatt aaggtagcag agcgcaaggt gaagcgtcgg
     1861 cgtgtgtata agtcgatcat cacctgtacg cagatactga aagaccgcga gaagatagcc
     1921 ggagagcata ttccaatcgt tccagtgtat ggcgaatggt cattcgctgg tgacaaggag
     1981 tgctacgagg gtgtggtaag gctgacgaaa gacggtcaac gccttcgtaa catgattatg
     2041 tcgttcaacg ccgatattgt tgctcggtca ccgaagaaga aaccgacctt cttccctgag
     2101 caaatcgaag gctacgaata catgtacggc ggaaatgatg actatccgta ctatctgcag
     2161 aacaggactg atgaaaacgg taacgacctg ccgattggtc caatctccta catggaaaac
     2221 cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg ccaccaacgc agtgaaagaa
     2281 gtggctagtc ttggcgtgga tgcgcaggcg gcaaatggtc aggtcgcttt tgataccgtc
     2341 aatcaactga acatgcgggc agaccttgag acatacgtgt ttcaggataa cctggctacc
     2401 gcaatgcgac gtgatggcga gatttatgcc tcaatggtca acgatattta tgacgttcct
     2461 cgtcatgtaa cgctgacact tgaagatgga agcgagaaag acgttcaact ctatgcgcaa
     2521 gttgtcgatt accagtccgg caatgtggtc acactcaacg acattcgcgg tcgatatgag
     2581 tgctatacag acgttgggcc atccttccag agtatgaagg aacagaaccg cgcagagatt
     2641 caggagttgc tcaccaaggt tccgcaaggt actccagagt tccagatgct gatgctgcaa
     2701 tacttcacgc tgcttgacgg taaaggcgtc gagatgatgc gagagtacgc gaacaagcaa
     2761 ctggtgatga tggggctgaa gaaaccagaa acacctgaag agatggagat ggtgcagcag
     2821 gcacaacaac agccgcagca gccatcagca gagcaaattc aggcgcaggg tatccttctg
     2881 caaggtcagg ctgaattgct caaggcagag aaccaacagg cgcagattca ggttgaagct
     2941 gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga ttgcagaaat attcaacaat
     3001 atggacctcg acaagcaggc agaactgcgt gagtacctca ggctcgtagg tcaattccag
     3061 caacagcgca gcaaagatgc tcgtgctaac gctgagctgc ttcttaaaga tgcagaccag
     3121 actcattcac aacgcatgga tttcgcgaat cttatgcgtc aagttcaaat cccctccggc
     3181 ggagtagccg agacacctca ataagagaga gttaatcatg gaccaaacca ccgacattca
     3241 ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg gcatctgctg atggcttagt
     3301 tgtcgataat gccaacgaca gcgcaggtca ggaagaaggc tttgagattg tccggaaaga
     3361 cgatgagaaa ccaaaacaag acccggcaac taatgctgaa tttgcccgtc gccgcatcga
     3421 acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg gttaagcgtg gagagttgcc
     3481 ggagcacctg cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc
     3541 cgaagatgca ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca
     3601 ggcaaacagt gaatggcaga tcaaggctat ggacgcacga agccaggctg tcgccgagca
     3661 gggtcgcaaa actcaggagt tcacccagca atcagcgcaa tacgtcgagg cggcccgtaa
     3721 gcactacgag gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt
     3781 catgcaactg gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc
     3841 cgccgctctc atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat
     3901 ggacgggcaa tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc
     3961 tcgagccaag cctgtttcag aagccccgtt acctgatgaa cccattcagg gacatgctgt
     4021 tgctgcaaat atctctgcga ttgaaaagca gatggaggcg gcagcaaaca aaggggatgt
     4081 agagacgtac cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat
     4141 gaaggtcaac tggtcacgta tgctctggat gaaatcatcg gaaccgtcca gaacctgacg
     4201 ccaatggcgt caaaagtgac aaaatacccc cctccggcag aatccatgca gcgttcaagc
     4261 aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact
     4321 ggcaacgcaa ccgggattct ggaactctcc gtgaagtgca acatgggcga tccggataac
     4381 gatttcttcg agcttcgtgc agatgacctg cgtgatgagc gttcttaccg tcgccgcatc
     4441 caggcatccg ccaaaaaact ggcgaataac attgagtcag cgattgccaa acaggcaact
     4501 gaaatgggct cgcttgttgt tcacgatacc cgcgcaattg gtccatcgac tggcctgcct
     4561 ggctgggatt ttgtgtctga tgcagagcga ctgatgttct cccgtgagct aaaccgcgac
     4621 atgggcatca gttacttcct gaaccctgac gattaccgca aagcaggccg caacctggta
     4681 gatggtgaca tcttcgggcg cgttcctgaa gaagcatatc gaaacggtac tattcagcgt
     4741 cagattgctg gctttgatga aattcttcgc tcaccgaaac ttccggcagt taccaagtca
     4801 accgcgactg gtgtaactgt ttctggtgcg cagaagttta agccgcaggc atacaccctt
     4861 gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg caacggtgac cgtatcctcc
     4921 accaccggat ttaagcgcgg cgacaaaatc agcttcattg gtgtgaagtt cctgtctcag
     4981 atggcgaaga atgtgctgac tgatgacgcg actttctcaa tcacccgtgt gatcgatggt
     5041 actcacatcg aaatcacgcc gaagccgatt gcgctggatg acgcgtcact gacaaaagaa
     5101 gagaaggctt acgctaacgt aaacacctct cttgctgata ccactccggt aaacgttctg
     5161 aacgtggcaa caaccaccgc taacgtgttc tgggctgatg actcaatccg cctgctgtct
     5221 cagccgatcc cggtaaccca tgaactgttt gctggcatga aaacgtcttc cttcagcatt
     5281 ccaggtattg gtgttaacgg catcttcgca acgcagggtg atatcaacac tctgtctggt
     5341 aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac gaccagaggc aattggtgtt
     5401 ggtctgccta accagaccgc gtgataacca gagggagctt cggctcccgt ttttatctgg
     5461 agacaagcat gacacacatg atctttcgtc atggcgacat gaagaagtgg aaaggcgttg
     5521 gctacgactt tgaaatcgtg aaagccgaag agcttcagga atatctggat gctggctggt
     5581 tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc agagccagaa gaaaaacagc
     5641 gtaaaaagcc tggtcgaaaa cctaaggcgg cagcagatga acctgacaac gaagggtgat
     5701 ttagttcttg cggcattacg taagctcggt gttgcatcaa atgccacgtt aaccgatgtc
     5761 gaaccgcagt ctatggaaga cggcgtcaac gaccttgaaa tgatgatggc tgaatggctt
     5821 ggcggtgatg cgtcacctgg tatcaacgtt ggctacattt ttgctgatgc agatgttgct
     5881 ccggatccgg gcgatgagca cggtttatca aataacgcta tcaatgccgt cattttcaac
     5941 cttgcctgcc gaattgctcc agattatgcg ctggaagcgt ctgcaaaact tataaccact
     6001 gccagatacg ggaaagagcg actcgtcaaa ctgtctgcaa tggacagggc aaaagcagct
     6061 aaatgtaagt ccggttatcc aaaccgtatg cctgttggta gtggtaacca gttggcgaag
     6121 tggaatggtt ggaattactt ccaccgaaag gaaccttgcg ataacgggag cgaataatgc
     6181 ctattcagca acttccgctt atgaaaggtg tcggcaaaga cttccgaaac gccgactata
     6241 tcgactatct gccagtgaat atgctggcta cacccaaaga aatcctgaac agcagcggat
     6301 atcttcgctc attcccgggc attgccaaac gttctgacgt gaacggtgta tctcgcggcg
     6361 tcgagtacaa catggcgcag aatgctgttt atcgcgtgtg tggcggcaag ctgtacaaag
     6421 gagaaagtga agtcggtgat gttgccggaa gtggtcgtgt atcaatggcg catggtcgga
     6481 catcacaggc ggtaggcgtt aatggtcaac tggtcgagta tcgctatgat ggcacggtca
     6541 aaaccgtctc aaactggcct acagacagtg gattcactca gtatgagtta ggttcagttc
     6601 gcgacattac gcgcttacgt gggcgttatg cgtggtcaaa agacggaact gattcatggt
     6661 ttatcactga ccttgaagac gaatctcatc ctgaccgtta cagcgcacaa tatcgtgccg
     6721 agtctcagcc tgacggcatc atcggtatcg ggacatggcg agacttcatc gtctgctttg
     6781 gttcatcgac tattgaatat ttctccctga ctggcgcaac caccgttggt gccgctttgt
     6841 atgttgcaca accatcgctg atggtgcaga aaggcattgc cggaacttac tgcaaaacgc
     6901 cattcgctga ttcgtatgcg tttatcagca atccggcaac gggtgcgccg tctgtgtata
     6961 tcatcgactc cggtcaggtg tcaccaattg ccagcgcgag cattgagaaa atcctccgct
     7021 cctacactgc tgatgaactg gctgatggtg tgatggaatc gctgcgattt gatgctcatg
     7081 agttgctgat tatccacctt ccgcgtcacg tcctcgtgta cgacgcatct tcaatcgcca
     7141 atggcccaca atggtgtgtg ctgaaaacag gtctgtatga cgatgtgtac cgcgctatcg
     7201 acttcgttta cgaaggcaat cagataacgt gcggcgataa gctggaatct gttaccggga
     7261 aactgcaatt cgatatcagc agccagtacg acaagcaaca ggaacacctg ctgtttactc
     7321 cactcttcaa agcggataac gccagagtgt tcgaccttga ggttgaatcg tcaactggcg
     7381 ttgcgcagta cgccgaccgc ctttttctct ctgcaaccac tgactgcatc aattacgggc
     7441 gtgagcagat gattgagcag aatgaaccgt tcgtttacga caagcgtgtt ttgtggaagc
     7501 gagtagggcg catcaggaaa aatgtcggct tcaaattgcg cgttatcacg aagtcacctg
     7561 tcactctgtc tggcgctcag ataaggattg agtaatggct gattcgaatc tcaatgtgcc
     7621 ggtaatcatc caagctacgc ggctcgatac atcagtcctt ccacgcaata tcttctcgca
     7681 gtcgtatctg ctttacgtta tcgcacaggg cactgatgtt ggtaacgtgg ctaacaaagc
     7741 caacgaggcc ggacagggcg cttatgatgc acaggtcagg aacgatgagc aggatgtgat
     7801 tctcgctgac catgagcagc gaatttctgc tgcggaagca acgcttgtta atcatgagga
     7861 gcgaatcaga caggcagaat caactcttca ggaccatgaa acacgaatag ctcagaatga
     7921 aagcgatatt tcgtcgcttg acacaagagt tcagtcgctg gagtcgcagg tttcagacca
     7981 tgaaacgcgc atcgatgctc tggagtatgc aaccacacgc aagaagtcag aggttgttta
     8041 ctctggtgta tctgtaacca tcccgacagc gccgaccaac cttgttagcc tgctgaaaac
     8101 gctcacaccg tcatccggct cgttggcacc attcttcgac accgttaaca acaagatggt
     8161 tgtgttcaac gagaacaaaa ccctgctctt caagttgtcg attgtcggca cgtggcccag
     8221 cggaaccgcc aacaggtcaa tgcagctaac cttttccggc tctgttcctg acacactggt
     8281 cagcagtcgt aatgcggcga caacaaccga caacatcctg ttagctacgt tcttcagcgt
     8341 ggataaagac ggctttcttg ccacaaatgg cagtacgtta accattcagt cgaatggtgc
     8401 ggcgtttact gccacaacca tcaagataat cgcggagcag tgatgattac attcaaacca
     8461 acgcgaaaca tcgacctgat agaagcagtc ggaaatcacc ctgacattat cgccgggagc
     8521 aacaacggtg atggatacga ctacaaacct gaatgccgtt actttgaggt gaacgtgcac
     8581 ggtcagtttg gcggcattgt ttactatcag gagattcagc cgctgacatt agattgccac
     8641 gccatgtacc tgccagagat tcgcggcttc agcaaggaaa ttgggctggc gttctggcga
     8701 tacattctga ctaacaccac cgttcagtgc gtaacatcgt tcgctgcacg caaattccga
     8761 cacggtcaga tgtactgcgc aatgattggc cttaagcgtg taggaaccat caagaaatac
     8821 ttcaaaggcg tggatgacgt gactttttac agcgccacac gcgaagaact aatcgacttc
     8881 ctgaatcacg ggagatagcc atgttatatg catttaagct gggcagaaaa ctgcgcggcg
     8941 aggaacctta ttgccctgaa aaaggcggga aaggtggcag ttctgataaa agcgcaaagt
     9001 atgcagcaga agcccagaag tatgctgcag acctgcaaaa tcagcagtgg cagacgatca
     9061 tgaaaaacct tgctccgttc acgcctctag cggagcagta tgttaaccag cttcagaacc
     9121 tttccagttt agaaggtcag gggcaggcac ttaatcagta ttacaactct aagcagtata
     9181 aagaccttgc agggcaggcg cgttaccaga gtcttgctgc tgcggaggcg acgggtggac
     9241 ttggttcgac agccacaagc aatcaactgg ctacgatcgc gccgacactc ggtcagtctt
     9301 ggttatcaaa tcagatgagc aattacaaca atctggcaaa cgttgggctt ggtgcgctgc
     9361 aaggtcaggc aaacgccggg cagacgtacg ccaacaacat gagcagcatt gcacagcaaa
     9421 gcgcagctct tgccgctgct aatgccaata aaccatcaag tcttcagact gcaattagcg
     9481 gtggcacgtc tggtgcgatt gccggtgcag gtcttgccag ccttttggga acatcaacgc
     9541 cttggggcgc tggtattggt gctggtatcg gattgcttgg ctcgttgttt taagggggaa
     9601 tcatggctac ttggcaagga acaaacggcg gattgttggc tggtatcggc ggcgtcaact
     9661 caaacgctcc gagcgtaaat gacatcggca atacgcttca gcttatcagg cagaacaatg
     9721 atattgagcg ttcaggcgct aacaatgttg ggctgactgc tttgcaaggt ctttcaggta
     9781 ttgcgggtgt ttttcagcag gaaaagcagg ctcagcggca gaaagaattt cagcaggcgt
     9841 acgctaatgc ttatgcgtct ggtgatcgcg gtgctttgcg tcagttggct actcaatatc
     9901 cagaccagat tgaatccgtt cgtaaaggca tgggattcat tgatgaagag cagcgtaatt
     9961 ctatcggcac cttagcggct ggcgcacgcc ttgcgtcatc gtctccagaa gcaatgcaat
    10021 catggctgca aaacaacgcc ggtgagttag ctcgtgttgg cgttaatcct catgacgtcg
    10081 ctcagatgta ccaacagaac ccgcggcagt tcggcgaatt tgtcgatcac ctggggatga
    10141 acagtctcgg gcccgaaaaa tactttgacc tacaggataa aatgcagggt cgccaggtta
    10201 ccatgcgcgg tcaggatctg gattcgcaaa ccgccgctcg gaatcaggca atcacaatgc
    10261 gcggacaaga tatccaggcg aatttaggtc agcagcgcat taatctggac gcagaaacaa
    10321 accgcattaa caacgaaaat aagcgccttg accggatgct atcagcagaa actaacgacc
    10381 tgaagcgcca ggaaatacag agccgcatag cagccaacaa ccagcagttg cagcagaagc
    10441 agcaagcgct aaatgatggc tacaaagacg gcatcaacac cctcacaacc agcatgttca
    10501 ctctgaacga tatcgttagt tctccttcac ttaagagcat tacaggctta cgtggagtaa
    10561 tccccaacgt tccaggctca caggctgcag acactcaggc acgacttgat acctttaaat
    10621 cacaagcata cctgacagcg gttcaggcca tgcgaggcat gggcgcactt tctgatgccg
    10681 agggcaaaaa gctcgaccag gctgttggtt cgctgcagaa ctcgcagagc gaggagtcct
    10741 ttcgtcgcaa cgctggcgtc atcctgaaca cgctcaacca gaagagtaat gaggcggttg
    10801 gtaagtacgt tcagcaaaac ggtatcaagc gagtggaagc gcctcaggct tctatagatt
    10861 acctgaagca gcaccccgag ctgtcaatcg acttcattaa tcgctacgga tatcttccat
    10921 ctttggggca gtaaatggct aattaccgtg atttgttaga gcaggctggc gcacgttacg
    10981 gtgtgccaga agggttgatg actgcactgg gtgccaagga gtcttcttac aaccctgccg
    11041 cagtaagctc cgccggggct gtaggattga ctcaggtcat gcctgggaca tggcgtgata
    11101 tgggttatac cgatgagcaa atgcaaaacc ccgaatatca ggctgacgct ggcgcgcgct
    11161 atctggcaaa gatgtaccag cagtttggta actggcgtga ctctcttcag gcttatcacg
    11221 acggtcccgg caacgttatg aaggcaaagc gtggtgaata tacgccagga cctgaaggcc
    11281 gcggttacgt tgatgatcgc tttgctcaat gggctggtga cccggtgaca gactcaacag
    11341 tcgaacagcg cgccacctct gcaaaggtac atcctcagca agaccctaac aacccgtttg
    11401 cacaactgga agtacagtca tccgaacaag tatcggcatc aggtgtgcag tcagacccaa
    11461 ataatccatt tgctcagatt gagcagcagg cagccagtca gcagccacct caacccgtaa
    11521 gttctgtcgc accgaaacct gttcagcaac aaacagttaa tcaggccaat aatgcaccaa
    11581 cacgggaaga accatcattg atgcaacaag ctggcgattg gctcactggt ggtcaaagtg
    11641 cagggcaaat tgcagaacag gctggtcgtg gtctggtaaa cataccattt gacgtattgc
    11701 agggtggcgc aagtctgatt aatgcaatca gtcaggggct tggggggcca aaagtattgg
    11761 atgatgttta tcgtccagta gacagaccga cagaccccta cgcgcaagct ggagagtcaa
    11821 taggcggtta tcttgttcct ggagcaggtg tagctggaaa catggtcatt ggttctctcg
    11881 ctgacgcggc gaatcaacgg ggtgattttg ccgaaaatgc cgctattaat gccggactta
    11941 gcattgctac gcatggcctg ataaatggcg ttacccgtgg tgttcgtggt gcatcaaata
    12001 taattagtgg caataaaaca tctgcacaga gagcgaccac tgcgccaaca gaaacatcac
    12061 cattctccgg tgatgccgct gcagcaacaa atcctgcggt tcatgccgca gaggcaagag
    12121 tagcacaagg tgtaccaatg acgcctgcga cgaagaaccc agaggaagtc gttcgcacag
    12181 tagcagcaca aaaaaggcca aatctcgctt catcgcttga tgaactagat atcaatcctc
    12241 aggctgaagt tctggagtct gctgaaaggc ttaatgttga ttcattactc ccttcacact
    12301 tttccgggaa cgagcaatac aaggcagttg agcaagcaat caagtcccgt gcgggttctg
    12361 ctctacaggt gcaggaaaat gaagcaatca ggcagctagc acagggcgcg ggtgagataa
    12421 ttgatcgcgt ttccggtgca aaagatgctc ttggtatgag cgacaagttt attgatacgg
    12481 tcaatggaag aatgtctgcg ctgatgaaac gaagcgacca gctttatcgc aatgttgaaa
    12541 aggcgatgcc tgcaggtgca aaaattgatg cgccatcaac aaggtcaatg ctcaaacagg
    12601 tggcagaaga tcttggcggg atgaaaaacc ttgaccctat tgaaaagaga gttgaggtag
    12661 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
    12721 gcagaaaaac ccaa
//
LOCUS       DBBBSB010000110        12695 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.110, whole genome shotgun sequence.
ACCESSION   DBBBSB010000110 DBBBSB010000000
VERSION     DBBBSB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.110"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACIUS0_003188"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACIUS0_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGB0220824.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACIUS0_003189"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACIUS0_003189"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGB0220825.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACIUS0_003190"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACIUS0_003190"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGB0220826.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACIUS0_003191"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACIUS0_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGB0220827.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACIUS0_003192"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACIUS0_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGB0220828.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACIUS0_003193"
     CDS             4025..4615
                     /locus_tag="ACIUS0_003193"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGB0220829.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACIUS0_003194"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACIUS0_003194"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGB0220830.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACIUS0_003195"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACIUS0_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGB0220831.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACIUS0_003196"
     CDS             complement(6551..6658)
                     /locus_tag="ACIUS0_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HGB0220832.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACIUS0_003197"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACIUS0_003197"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGB0220833.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACIUS0_003198"
     CDS             7648..7875
                     /locus_tag="ACIUS0_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGB0220834.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACIUS0_003199"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACIUS0_003199"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGB0220835.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="ACIUS0_003200"
     CDS             complement(9447..10703)
                     /locus_tag="ACIUS0_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HGB0220836.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="ACIUS0_003201"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="ACIUS0_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGB0220837.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="ACIUS0_003202"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="ACIUS0_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGB0220838.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="ACIUS0_003203"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="ACIUS0_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGB0220839.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3241 c         3199 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DBBBSB010000111        12389 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.111, whole genome shotgun sequence.
ACCESSION   DBBBSB010000111 DBBBSB010000000
VERSION     DBBBSB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.111"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACIUS0_003204"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACIUS0_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGB0220840.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACIUS0_003205"
     CDS             complement(2005..2928)
                     /locus_tag="ACIUS0_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGB0220841.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACIUS0_003206"
     CDS             3145..4488
                     /locus_tag="ACIUS0_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGB0220842.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACIUS0_003207"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACIUS0_003207"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGB0220843.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACIUS0_003208"
     CDS             6508..6732
                     /locus_tag="ACIUS0_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGB0220844.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACIUS0_003209"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACIUS0_003209"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGB0220845.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACIUS0_003210"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACIUS0_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGB0220846.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACIUS0_003211"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACIUS0_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGB0220847.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACIUS0_003212"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACIUS0_003212"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGB0220848.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="ACIUS0_003213"
     CDS             10315..10971
                     /locus_tag="ACIUS0_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGB0220849.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACIUS0_003214"
                     /pseudo
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="ACIUS0_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11503..12389
                     /locus_tag="ACIUS0_003215"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="ACIUS0_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT         3230 a         2903 c         3110 g         3146 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca taaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DBBBSB010000112        12336 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.112, whole genome shotgun sequence.
ACCESSION   DBBBSB010000112 DBBBSB010000000
VERSION     DBBBSB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.112"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..460
                     /locus_tag="ACIUS0_003216"
                     /pseudo
     CDS             1..460
                     /locus_tag="ACIUS0_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            625..4743
                     /gene="pic"
                     /locus_tag="ACIUS0_003217"
     CDS             625..4743
                     /gene="pic"
                     /locus_tag="ACIUS0_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HGB0220850.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4894..5136)
                     /locus_tag="ACIUS0_003218"
                     /pseudo
     CDS             complement(4894..5136)
                     /locus_tag="ACIUS0_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5175..6125)
                     /locus_tag="ACIUS0_003219"
                     /pseudo
     CDS             complement(5175..6125)
                     /locus_tag="ACIUS0_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6176..6751)
                     /locus_tag="ACIUS0_003220"
                     /pseudo
     CDS             complement(6176..6751)
                     /locus_tag="ACIUS0_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6755..7155
                     /locus_tag="ACIUS0_003221"
                     /pseudo
     CDS             6755..7155
                     /locus_tag="ACIUS0_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7558..8139)
                     /locus_tag="ACIUS0_003222"
     CDS             complement(7558..8139)
                     /locus_tag="ACIUS0_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220851.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8186..12043)
                     /gene="sigA"
                     /locus_tag="ACIUS0_003223"
     CDS             complement(8186..12043)
                     /gene="sigA"
                     /locus_tag="ACIUS0_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HGB0220852.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3141 a         2867 c         2962 g         3366 t
ORIGIN      
        1 gggcttcgtc tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tgtggtgacg
       61 agtctcatcg tctatggaaa cgacattcgt gctggatgca ctggagcagg cgttgtgggc
      121 ccgtcgtccg tctggcacca tccatcacag cgataaaggc tctcagtatg tgtcactggc
      181 ctataaggcg cgactaaaag aagcaaaact gctggcatca acagggagta ctggtgactc
      241 gtatgacaac gcgatggcag agatcatcaa gggcctttac aaagcggagg taatacaccg
      301 taagagctgg aaaaaccgga cagaagtgga gctggccaca ctcacgtggg tggactggta
      361 taacaatcga cgattgctgg aaaggctggg ccatatccca ccggcagaag cagaaaaagc
      421 ttattatgct tccatcggaa atgatgatct ggcagcctga gttcacagat aaaacactct
      481 ccaggaaacc cggggcggtt cagttcacaa aaacacatta atgcagtaac tatattttcc
      541 tttctgatga taacgtcggg ttatcattag cttcttcagc tattttactt ttatatccct
      601 tgtaaacatc atggagaatc catagtgaat aaagtttatt ctcttaaata ttgccccgtc
      661 accggggggc ttattgctgt ctctgaactt gcccgcaggg taataaaaaa gacatgccga
      721 agattaacgc atattcttct ggctggcatt ccagcaatct gtctgtgtta ctctcagata
      781 tcccaggcgg gtattgtccg ttccgatatt gcctatcaga tttatcgtga tttcgccgaa
      841 aacaaagggc tttttgtacc tggtgccaat gatattccgg tatatgataa ggacggaaaa
      901 cttgtgggaa gactgggtaa agccccaatg gccgatttca gcagtgtgag ctcaaatggc
      961 gttgctacgc ttgtatcgcc tcagtatatc gtcagcgtaa agcataacgg aggatatcgg
     1021 agtgtgagct ttggtaatgg gaaaaataca tattcccttg ttgaccgtaa taaccaccct
     1081 tctattgact tccatgctcc acgtctgaat aaactggtta cagaagttat tccctcagcg
     1141 gtaacatcag aaggaaccaa agccaatgct tataaataca ctgaacgtta caccgctttt
     1201 tatcgggtgg gtagtggtac gcagtacact aaggacaagg acggaaattt agttaaggtt
     1261 gccggtggat atgcttttaa aacaggagga accacaggag ttcctctgat atctgatgca
     1321 acaatagtct ctaatcccgg gcaaacttat aatcctgtaa acggcccttt acctgactat
     1381 ggagcccctg gggatagtgg ttctcctttg tttgcttatg ataaacaaca aaaaaaatgg
     1441 gttattgttg ctgtattaag agcatatgca ggtattaatg gtgctacgaa ctggtggaat
     1501 gtcataccaa cagattatct gaaccaggtt atgcaggacg atttcgatgc ccccgtagac
     1561 tttgtttccg gactgggccc cctgaactgg acatacgaca aaacatcagg cacaggtacc
     1621 ctgagccagg gcagtaaaaa ctggaccatg cacgggcaga aagacaatga cctcaatgcc
     1681 ggtaaaaatc tggtattcag cgggcagaat ggtgcaatta tcctgaaaga cagtgtgact
     1741 cagggtgccg gttatctcga atttaaagac agttacaccg tatctgctga atccggaaaa
     1801 acatggacgg gtgccggcat tattactgac aaggggacga atgtaacctg gaaggtcaac
     1861 ggcgttgccg gtgacaactt gcataagctg ggggaaggaa ccctgaccat aaacggaaca
     1921 ggtgtaaacc cgggaggact gaaaacggga gacggtatcg ttgtacttaa ccagcaggca
     1981 gacactgcag gtaatatcca ggccttcagt tcagtgaacc tcgccagcgg acgtccgacc
     2041 gtggtgctcg gggatgcccg tcaggtcaat ccggataaca tttcatgggg ataccgggga
     2101 ggtaagcttg accttaatgg taatgccgtt accttcaccc gactgcaggc tgctgattac
     2161 ggggcggtga ttacaaataa tgcacagcaa aaatcccagc ttttactgga tcttaaggct
     2221 caggatacaa atgtcagtga accgacgatt ggaaatatat ccccctttgg tggtaccggc
     2281 actccaggaa acctgtacag catgatactc aacagccaga cccgcttcta tattctgaaa
     2341 tctgccagct atggtaacac tctgtggggg aacagcctga atgatccggc tcagtgggag
     2401 tttgttggca tgaacaaaaa caaagcagtt cagacagtaa aagataggat cctggccggg
     2461 cgggcaaaac aacccgttat ctttcatggt cagctgaccg ggaatatgga tgtcgccatt
     2521 ccacaggtgc cggggggaag aaaggtaatc tttgatggta gcgtgaacct gccggaaggt
     2581 accctgagtc aggacagtgg caccctgata ttccagggac atccggttat ccatgcctcc
     2641 atcagtggca gtgcaccggt cagcctgaac cagaaagact gggaaaaccg tcagtttaca
     2701 atgaaaacac tgtcgctgaa agacgctgac ttccatcttt cacgtaacgc ctcgctgaac
     2761 agtgacatta agtcggataa cagccatatc acactgggaa gtgacagggc atttgtggat
     2821 aaaaatgacg gaacaggaaa ttatgtcatt ccggaggaag gtacctctgt cccggacacc
     2881 gtgaatgaca ggagccagta tgaagggaat attacgctga accataactc agccctggat
     2941 atcggcagca ggttcaccgg ggggattgac gcttatgaca gtgccgtcag catcacctct
     3001 ccggacgtcc tgttgacagc cccgggtgct tttgccggca gttcactgac agtgcatgat
     3061 ggcggtcatc ttacagcact gaacggtctt ttcagcgacg ggcatattca ggccggtaag
     3121 aacggcaaaa tcaccctgag cggtacaccg gttaaagata cggctaatca gtatgcccct
     3181 gctgtatatc tgacggacgg atatgacctg accggcgata acgcagcact ggaaattacc
     3241 cgtggagcac atgcttccgg tgatattcat gcctctgcgg catcaacagt taccatcggg
     3301 tctgacacgc cggcagaact ggcttctgcg gaaacggctg catcggcgtt tgccggcagt
     3361 cttcttgagg gctataacgc agcattcaat ggtgccataa ccggtggcag ggctgatgtc
     3421 agtatgcata atgcactgtg gactctgggt ggggactctg ccatccacag tcttaccgtc
     3481 agaaacagcc gtattagttc tgaaggagac cgtacattcc gtaccctgac ggtgaataaa
     3541 ctggatgcaa caggcagtga ttttgttttg cgtacggacc tgaaaaatgc cgataaaatt
     3601 aatgtgactg aaaaagccac tggttcagat aacagcctga acgtcagctt tatgaataat
     3661 cctgctcagg gacaggccct gaatattcct ctggtcacgg caccggcggg aacttcagca
     3721 gagatgttta aggccggcac ccgggtgaca ggtttcagtc gggtgacccc aaccctgcat
     3781 gttgatacca gtggtggcaa tacgaagtgg atactggatg gttttaaagc ggaggctgat
     3841 aaagccgctg ccgcgaaggc tgacagtttc atgaatgccg ggtataaaaa cttcatgacg
     3901 gaagttaaca atctgaacaa acgtatgggt gacctgcgtg acacaaacgg tgatgccggt
     3961 gcctgggcgc gcatcatgag tggtgccggt tctgcagacg gtggttacag tgataattac
     4021 acccatgttc aggtcggctt tgacaaaaaa catgaactgg acggtgtgga cctgtttacc
     4081 ggtgtcacga tgacctatac cgacagcagt gcagacagcc atgcattcag cggaaagacg
     4141 aaatcggtgg ggggcggtct gtatgcttca gcattgtttg agtccggtgc ctatatcgat
     4201 ttgattggta aatatattca ccatgacaat gattacacag gtaactttgc tagcctggga
     4261 acgaaacact acaacaccca ttcctggtat gccggtgctg aaacgggtta ccgctatcac
     4321 ctgacagagg acacgttcat tgagccgcag gctgaactgg tttacggcgc cgtgtccggg
     4381 aaaacattcc gctggaaaga cggtgatatg gacctgagca tgaagaacag ggacttcagt
     4441 ccgctggttg gaagaacagg ggttgaactg ggcaagacct tcagtggtaa ggactggagt
     4501 gtgacggccc gtgccggaac cagctggcag tttgacctgc tgaataatgg agagaccgta
     4561 ctgcgtgatg cgtccgggga gaaacggata aaaggagaga aggacagccg gatgctgttt
     4621 aatgttggta tgaatgcgca gataaaggac aatatgcgct ttggtctgga gtttgagaag
     4681 tcagcctttg gtaaatataa cgtggataat gcggtaaacg cgaatttccg gtatatgttc
     4741 tgatgtgggg gtaaagggct gccggaaggc agcctttctc agagactctt aaaatataaa
     4801 actgaaggtc ttcggtaagg gggagtgtat tagacgtcaa ctagttttgc cgactggcgc
     4861 gagcctgttt aagattctgt gtaaatgcct ttctcagaag tgaccgtcca ggcggtcacc
     4921 gaactcgata ataaagcggc tcattgccat tcgccagtcc ttcaacggca tcgtccattt
     4981 ctgggacgca gactggattg ccagccacac cacttttttc accgagtcgt ctgtcgggaa
     5041 cactttacgc tttttgatcg catggcggat cacgctgttt agcgactcga tggcattcgt
     5101 cgtatagatc actttgcgga tgtccgttgg ataagcggta agcgtacagc gagggccgta
     5161 ttgacgggga tgtgttattc agctggcagt gctatgcgcc acggaagcag ttcgctgacc
     5221 cggttgaccg gccagtctgc tatgacgcca agcacatggc gaaggtagct ttctggatcc
     5281 acgtcattca gtttgcacgt cccgatcagg ctgtacagta gcgcgccccg ctcaccacca
     5341 tggtcagagc cgaagaacag gaagttttta cgacccagac tgaccgcccg cagggcattt
     5401 tcagcgatgt tgttgtcgat ttccacccag ccattactgc agtacacgtt cagggccttc
     5461 cactgtttca gtaggtatgc gaacgctttt gccgtatccg agtgacgcga cagtgttttc
     5521 atctgttgct gtatccggtc atacagtgac tgcatcagtg gcgcggctct tgcttttctt
     5581 gccgccagac gctgttctgc tgaacatccc cggacttctg cctcgatggt atacagttca
     5641 ccgatacgct gcagggcttc cgtggtgatg tcggttggca ctcttgcatg cacatcgtgg
     5701 atttttctcc gggcatgggc catacacgca gcttccgtta ttctgccgga ttcgtataac
     5761 gcccggtaac caccgtaagc atctgcctga agcacaccgc tgtaaccggc taggtgattc
     5821 tgtggatgga tacctttccg gtccgggctg tacgcgaacc agaccgccgg gggcatctgt
     5881 gaaccggcgt tacggtcatc acggacatag acccacagcc gggcagttct tgttttacca
     5941 ctgcccggct cctggaccgg aacggggata tcatccgcat gaactttacc gggcatcagt
     6001 acatactggc gcaggacgtc atacagcggc tccagcagtt cagcaacggc accggtccag
     6061 cgacccagtg tggcacggct cagctccacg ccctgacgac ggtatatttc tgactggcgg
     6121 tataactgtc aacgacggat gaaaagtggc tcttccgcaa cctgggtgga gattttcatg
     6181 ccagtggact cattaccctc tgcctgagta gttcaaatcc tgctcgacca tacatctggc
     6241 gtttcagcat cttcttcagg cgatttacat gaccttcgac aacaccatta ctccagcgac
     6301 tactgattgc ctcacatata gctgcagcat cagcttccat ccccgccgcc acgcgccgaa
     6361 attctgctga accgctttcg tggactcgag tgaaccaact gcttagctgt gatttatttt
     6421 gggttttcag aatacggtag aactcgagtg ccagttgctg cgctattttc agctcaggtt
     6481 ctttttcaca catcagacta ataaatcggg aagcataatt ttcttcatcc ctggttattc
     6541 tccagggcat caaccagcgg cttacccggg agactgaagg aagtctgacg gcagtagtaa
     6601 ccggtgggtt ccagccttta cgccatttgg caaccgcatc cctgacgatg gtttcactgc
     6661 ctgtaaatcc ccgggccacc atttcccgcc atatccggct ggcgttataa ttaccacttt
     6721 ctcgttgctc ttttaaccac tccctccatg gtgtcagcga aaaacgaaac cgctgttgaa
     6781 atccctggaa agctggttgc ggaaaagatg aagacccagt cgcgacactc agagttggcg
     6841 aaggcgttcg cgtacgcact taaccagtgg ccggcactga cgtactatgc gaacgatggc
     6901 tgggtggaaa tcgacaacaa catcgctgaa aatgccttgc gggcggtcag tctgggtcgt
     6961 aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc ggggagcgct actgtacagc
     7021 ctgatcggga cgtgcaaact gaatgacgtg gatccagaaa gctaccttcg ccatgtgctt
     7081 ggcgtcatag cagactggcc ggtcaaccgg gtcagcgaac tgcttccgtg gcgcatagca
     7141 ctgccagctg aataacacat ccccgtcaat acggccctcg ctgtacgctt actcatcacc
     7201 tgctattaat ccccgagtca gtacttcgcg gcctgacgcg atcaccctac tcagtacttc
     7261 agagcccgcc tccggtacct gcctggcgat cgcccctgtg caacaaaata aatcaaccgt
     7321 tatcaaacca cgatcgttcg tatgcgccgc cgatgacgcg cgcctgaaaa acgcgtcaat
     7381 atgagttccc cggaagctta cgataatacg cttatgaaca ttacaacacg ctcatgcgga
     7441 acctggatgg gggaaggaac ggtgaagctg ttagcgaatg accggcgttt atacaaacgc
     7501 cggtcatgat gaatgatatt tcagaatata cctcgggcta aaataccggg acagagatca
     7561 ggcgtgatta atctgcagca tttttaataa cagaagtact gagcttactt tacctttgat
     7621 gatgtaatag ttatttccgt cggtactcat ttttccctga aataatgtca cggatgtttc
     7681 ttcctgctga tcctgcaggc gcagtcgtaa ggtcatattt ttaatgagct gagatccggg
     7741 tggcaagggg tatttgctgt cagatactgg gcgactgagt ctgatgtggc gcgtatacag
     7801 cccgttttcg tacagggtgg cctctgttat ggtgtcagtg accagcatgt gggagacaat
     7861 gctgtattct tttttaaagc acccaccaga aacggtgatt gtaatgataa tggtcttctc
     7921 gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg agaactcagt
     7981 tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt ctttttttat
     8041 tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa agctaataat
     8101 gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctccgg tagaagaagg
     8161 gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg ttgatcgcgt
     8221 tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag cgaagattat
     8281 cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt tcacctttga
     8341 tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac aggtcaaact
     8401 ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag gatttaccca
     8461 catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg gtcacgctca
     8521 ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc agttctgcct
     8581 gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca gcacccacat
     8641 acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc gagtactcat
     8701 tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca aatatagcgg
     8761 aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta ctgctggcat
     8821 gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc agctcatgtt
     8881 ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacca gaagctgaac
     8941 ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca cgcagatccc
     9001 ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta ccggacatat
     9061 acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg gtcagggtcc
     9121 acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg ctgaaaccta
     9181 tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct gttatcaggt
     9241 caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac agactgttat
     9301 tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca gactggctca
     9361 gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact gtcacagtat
     9421 ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt tttaatgatt
     9481 gcagggctga ctgtccgttt actctccata aggtgttata catgctggct gaggctgcag
     9541 gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct gtagccagcg
     9601 gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca aacagaatag
     9661 atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga gcctttaacg
     9721 tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg ctgtatttat
     9781 tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata gctttatcta
     9841 catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg tctgtattaa
     9901 ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt attttgctgt
     9961 tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg gagcgaagct
    10021 gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg tcaatgtatg
    10081 cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct tttacattag
    10141 catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa ttaaatttac
    10201 gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta tattccgtat
    10261 tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag tctttttgac
    10321 ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt tctgtcgcat
    10381 gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata tcgactgagc
    10441 catcaaagat cagattggca ttattgttat gttgctgggt agtattgata atatgattta
    10501 tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta gtaagtgata
    10561 atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt gctgcgattt
    10621 tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg gtaaaataga
    10681 tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc gtacctttac
    10741 ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca gtcccattac
    10801 ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta cctattttat
    10861 gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta ttatcaatat
    10921 ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc tggttcttat
    10981 cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat acccctcctc
    11041 ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt ttaccatcca
    11101 gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta ttaaataacg
    11161 agtagtttgt ccaggctata ttggtactac tgataaaacc aactgtactg gtaacaccat
    11221 ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca ctatcgccaa
    11281 aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg tttttccagt
    11341 ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca aacagatatt
    11401 gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca attatttgtt
    11461 ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa agtgagaagt
    11521 cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga tgaaccgaaa
    11581 agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat gactgtgttg
    11641 ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca cctccaattg
    11701 atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt tctggaaaat
    11761 gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt ctgaactctc
    11821 ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata tccagcattg
    11881 ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata ccgaggataa
    11941 ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca gcaactaatc
    12001 cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa ctccgatatt
    12061 tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag tcatggtacg
    12121 taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca gatgtaacaa
    12181 aatgaaaaaa aagataagca caagtgattg attggttaga tattgatcac ttggtgtttg
    12241 tattttgaat tttgcgatca aaatttgatc tttgttgttt ccggtcaatc ctttacaggt
    12301 tttctgtatt atccggggga gttaagtctg tcgatg
//
LOCUS       DBBBSB010000113        12320 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.113, whole genome shotgun sequence.
ACCESSION   DBBBSB010000113 DBBBSB010000000
VERSION     DBBBSB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.113"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACIUS0_003224"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACIUS0_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGB0220853.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACIUS0_003225"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACIUS0_003225"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGB0220854.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACIUS0_003226"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACIUS0_003226"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGB0220855.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACIUS0_003227"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACIUS0_003227"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGB0220856.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACIUS0_003228"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACIUS0_003228"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGB0220857.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACIUS0_003229"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="ACIUS0_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7850..7903
                     /locus_tag="ACIUS0_003230"
     CDS             7850..7903
                     /locus_tag="ACIUS0_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220858.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACIUS0_003231"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="ACIUS0_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGB0220859.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="ACIUS0_003232"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="ACIUS0_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGB0220860.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACIUS0_003233"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="ACIUS0_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGB0220861.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="ACIUS0_003234"
     CDS             10967..12166
                     /locus_tag="ACIUS0_003234"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGB0220862.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3010 a         3126 c         3306 g         2878 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggtgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga
//
LOCUS       DBBBSB010000114        12201 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.114, whole genome shotgun sequence.
ACCESSION   DBBBSB010000114 DBBBSB010000000
VERSION     DBBBSB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.114"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..602)
                     /locus_tag="ACIUS0_003235"
     CDS             complement(<1..602)
                     /locus_tag="ACIUS0_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGB0220863.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1138..2037
                     /locus_tag="ACIUS0_003236"
     CDS             1138..2037
                     /locus_tag="ACIUS0_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGB0220864.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2086..3099
                     /gene="yhjD"
                     /locus_tag="ACIUS0_003237"
     CDS             2086..3099
                     /gene="yhjD"
                     /locus_tag="ACIUS0_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGB0220865.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3510..4832
                     /locus_tag="ACIUS0_003238"
     CDS             3510..4832
                     /locus_tag="ACIUS0_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220866.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(5014..7074)
                     /locus_tag="ACIUS0_003239"
     CDS             complement(5014..7074)
                     /locus_tag="ACIUS0_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGB0220867.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7144..7911)
                     /gene="pdeH"
                     /locus_tag="ACIUS0_003240"
     CDS             complement(7144..7911)
                     /gene="pdeH"
                     /locus_tag="ACIUS0_003240"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGB0220868.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8143..9072
                     /gene="kdgK"
                     /locus_tag="ACIUS0_003241"
     CDS             8143..9072
                     /gene="kdgK"
                     /locus_tag="ACIUS0_003241"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGB0220869.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9168..10664)
                     /locus_tag="ACIUS0_003242"
     CDS             complement(9168..10664)
                     /locus_tag="ACIUS0_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGB0220870.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10885..12171)
                     /gene="dctA"
                     /locus_tag="ACIUS0_003243"
     CDS             complement(10885..12171)
                     /gene="dctA"
                     /locus_tag="ACIUS0_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGB0220871.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2927 a         3312 c         3002 g         2960 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttaccgata taaatagtta cagctaaacg cctgaaatta
      781 catgtcgagg gcactattta aaacaatttt gaggatttcc ttatattggt ggttagtacg
      841 catgcaatta aaaatgaaat tccgcgacta caagccaaaa taacaaatgg caatgagaca
      901 aaaataagca caaatagcca atacgtcctc tattcacttt aaagggaatc actgaaaaat
      961 acgctctgtt taaggggatt cacctttctc ataaagctat tccgcccttt tcctgctgag
     1021 aaatcgccac attcgtcatg acaacattgt gaaacccggc attagatgtt agaaaaaaca
     1081 ataaacaatg cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg
     1141 gataaaattc acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc
     1201 gcggcggatt tctttgcttt gccaaaggga agtgtttcgc gccagataca ggcactggaa
     1261 catcaacttg gcacccagct tctccagcgc actacgcgac gggtcaaact cacgccagaa
     1321 ggcatgacct attatcaacg ggcaaaagat gtgttgagta atctcagcga actggacggt
     1381 ctgtttcaac aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgtcagga
     1441 atcgcgaaaa acctgttact gccgcgtctg tcggaatttc tctatcagca tccgggaatt
     1501 gagctggaac tgagtagcca taaccgtccg gtagatattc ttcatgatgg ttttgattgc
     1561 gtgatacgca ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaacta
     1621 accatggtca actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaagcccc
     1681 gatgatctga cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta
     1741 ggttttgagg ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg
     1801 gtaaacagta gcgaaaacta tctcgccgcc ggtcttgccg gtctggggat tattcagatc
     1861 ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt gttgccaggc
     1921 taccgtgccg agccgctctc cctttcgctg gtttacccgc agcgtcggga gctttcccgg
     1981 cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga aagagcacct ggactgaccg
     2041 actatacttt ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac
     2101 gagatcaaac gtcccaccca ggatctggag cacgagccga ttaagcagtt ggataatagc
     2161 gagaaaggcg gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc
     2221 caacgccaac cggttattgc gcaccttatt cgcgcgacag aacgctttaa cgatcggctg
     2281 ggtaaccagt ttggtgcggc tatcacctat ttctcatttt tgtcgatgat cccgattttg
     2341 atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat
     2401 atcttcgaca aaattctgca aaacatcagc gatccgacac tagccgccac gttgaaaaac
     2461 accatcaaca ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggca
     2521 ctttattccg gcatcaactg gatgggtaac ctgcgtgaag cgattcgtgc ccagtcgcgc
     2581 gatgtctggg aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgtgat
     2641 tttatttcac tgattggttt gttgattgcg ctgattgtga cgctttcgat cacctcggtt
     2701 gccggttcgg cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg
     2761 aaaccgacgt ggcgattgat tggcctggcg atttccatct tcgccaacta tctgcttttc
     2821 ttctggatct tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga
     2881 acatttctgg ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccctgcca
     2941 tcgctgatga aatccccctc tggcgcagca tttggttccg tgctggggct gatggcattt
     3001 ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat
     3061 aaagacgacc cgcgaatgcc agggaaaacg cagccttaaa ataggccgga tgcggcgtaa
     3121 acgccttatc cggcatacga ttttattcgg cctggctccc cgtaggccgg ataagatgcg
     3181 ccagcatcgc atccggctat aatgcgcaca taacctcttg aaactcttcc ccagagcctc
     3241 ttcagccatc tatttgggag caaacaattt cactccaact cataacccca gcatataaat
     3301 ccagttggta acttttattt aacctgaaac cagttttatc cactatttat aaaattatgt
     3361 gaagcatttc atagaagaaa aatcactggc ctaaacatta tccccttttt gcctggtttt
     3421 tgaccatttc cgcgatttgt tacacattga aatatcactt ttgctgtgcg taatatggct
     3481 attcgttagc caaaaaataa gaaaagatta tgcaagcaac agccacaaca ctcgaccacg
     3541 aacaagaata cacgccgatc aactcgcgta ataaagtcct tgtcgcctct ctcattggca
     3601 cggccattga gttcttcgac ttttacattt acgccactgc ggccgttatt gtgtttccgc
     3661 atatcttctt cccgcagggc gatccaacgg cagcaacgct acagtcgctc gccaccttcg
     3721 ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt tggtcatttt ggcgatcgcg
     3781 ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat ggggatttcg accgtggtga
     3841 ttggtctgct gccgggctat gccacgattg gtattttcgc cccactgctg ctggcgctgg
     3901 ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg cggcgcggcg ctgctggcga
     3961 ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc cttcccacag ctaggcgcac
     4021 cgattggctt cttctttgcc aacggcactt tcttgctgct ttcctggcta ctgaccgatg
     4081 agcagtttat gagctggggc tggcgtgtgc catttatctt ctcggcggtg ctggtcatta
     4141 tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt gtttgagaaa gtcgctaaag
     4201 cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac caaacatgtt cgcgtaaccg
     4261 tactgggtac gttcattatg ctggcaacct atacgctgtt ttacatcatg acggtctact
     4321 ctatgacctt cagtaccgcc gccgcgccag ttgggcttgg cctgccgcgt aacgaagtgt
     4381 tgtggatgct gatgatggca gttattggtt ttggcgtgat ggtgccagtc gccggattac
     4441 tggctgatgc ctttggtcgc cgtaaaagca tggtgatcat caccacgctg atcatcctgt
     4501 tcgcgctgtt cgcctttaac ccgctgctcg gttctggcaa cccgattctg gtttttgcct
     4561 tcctgctgct ggggttaagt ctgatggggc tgaccttcgg gccgatgggt gcgctgttac
     4621 cagagctgtt tccgacagaa gtgcgttaca ccggagcatc gttctcttac aacgtatcgt
     4681 cgattctcgg ggcttccgtt gcgccgtata tcgcagcctg gttgcaggct aactacgggt
     4741 taggtgcggt ggggttatat ctggcggcga tggctggctt gacgttaatc gccctgctgc
     4801 tgacccatga gacgcgacat cagtcgttgt aataagtgtg aaaagcctga tgcgctacgc
     4861 ttatcaggac tgaatttcca tacaatatat tgaattcaca aggatttgta ggccggataa
     4921 ggcgttcacg ccgcatccgg catgaacaaa gcacactttg ccaaaaatct aaatctgttt
     4981 aattgccgga tgtcagacat ccggcaatta tcatcacttc ttcatctgcg acaatatcgt
     5041 ccgacactga ttagcctccc cttcggaagg tgagatcagt gccagtaacg ccgccgccgg
     5101 tgttaccagc gttgccagtg ccgcagcaac agcaccacgg gcaatcagcg gtccggcttt
     5161 caccccagcc tgcggatttt taaacgtccc acgcacatac agcggcgaac gcagtgtgat
     5221 aatccgaatc cctttactct ccggatcaat agtcaaatcc agctgttccg aagcaaaact
     5281 tgccgtgccg gtaacattaa tcaacgcgtt ctcagtatcg aaagcaaaaa tctgcgggcg
     5341 cgccacgccg ttggcaatat tcagattcgc cgccgcgcag ttcacccgca cctcatcgtc
     5401 accaaatatc gcaccgacaa tgtagttgcc gacattcagc ccaacaatct ccatcaggtt
     5461 gcggctcacc agcccgtcat tcatcaacag tttcaggttg ccgttactgt tgcctaaaag
     5521 cgccgccacc gagttaccgc taccgcgtag ttccgcgtca ccgttcattt cccccagcgt
     5581 cttctgcatc agttccacat cgggcatcag ttctttcagt ttcagtcgac gagcctgaat
     5641 atctgcccgc ccctgcatcg gctttttatc gccttccaga tgaatattcg ccgcaatgct
     5701 gccgcccgcc atgccaaatt tcagcggttg caggcgcagg tcagcatttt tgaggatgat
     5761 atgagtagaa agatcgctaa tcggcaggct actgccatgc tcaatgcgcc gccctttgaa
     5821 gcgaacatcg gcatccataa cgtcccattt atcggtttcg aagcggtcat aaggcagcac
     5881 tttgcccgca ggctgaacgc ttttttcgcc cttcttctgt tcagaccgtt tcgacttttc
     5941 tgcccctttc ccggaatcaa cgccaatcaa cggtcccagg tccgccagcc gcaattgccg
     6001 cgactcgaca tcaccttcca gttttggtcg tggctttccg gtggtgtaga ccagagaacc
     6061 gtggatatcg ctatcgccaa ttcgcccatt aaaaccgcga taatcaaaga ccgacgattt
     6121 ttcagtgtcg attttcgcta ccagccgacc atccgtttca aacggcgggg tatcgggcag
     6181 cagaacgccc gtcagttcat agagatcacc cagtgaatcg ccagaaaatt taagccgtaa
     6241 atcgacaccg cccatcttca ttgggtcatt cacgacgcca tcaaaagcaa cacgggtatt
     6301 acctgaacgg aaatcagcct gtaccggaaa cggcgtccct tcgccacgca gcgccagcat
     6361 accgcctatt tttcccgtac ccgtgagcgg ttcaccgtta taacgtccct gcgccttcag
     6421 gccaaaaatg taatcgccca ccttttcttt atccgcttta cctttcgatc cagtaacttc
     6481 gctgaatggc agcggcttgc ctaacggatc aacaaaaatt tccagatccg ctttgcttac
     6541 tttgtcatca atggcgatcc gcccttgatc gaaaagaata ttatccagcc gaaacgacca
     6601 tgccgacggc tttgcattcg cgtctttgtt gtcatcgttg gcaagattaa acgtccagtt
     6661 attgttcttt tcagagaggc gaatcaggcg cgcgtcgggc ttttcgagct tgatccacgg
     6721 cagccagacc gttttggtca gcagcgccag cggtgccagc gttgcctcta ctcgcggcaa
     6781 atgcaccatc gtgacttcgg gaatatccgg tggattacca agaatgatgt cttctgcatg
     6841 tacatgaggc cacggcaccc agctgcgcca gccagtttct tgtttttgcc gctcccacac
     6901 cacgcccaga ttgccacgga tagcgaacgg acgattcaac tccgcagaga ctttctggtt
     6961 gatggtcggt ttgagtcgat tccagtcaaa agttgcaatc aaaatgatcg ccacgacaat
     7021 caacaacaag aaagcccctg aaatcgcagc ggttattttg cctgccttgc tcatcgttcg
     7081 cttcctcata ttcttcctgt gccagtccta aagatagtcc agcccggcgg aaaatgaggc
     7141 agcttatagc gccagaaccg ccgtattcag cgtttccatc ggtgccgggc gtgaaagaaa
     7201 ccagccttgt gcggcgaatg cgggcgagtt ctgaacatca cgccactctt ccggtgtttc
     7261 tacgccctcg acaatcaccc cgcgacaata gcgattcatt agatgtaaaa gctgagaaaa
     7321 gagtgtgcgg ccttccggcg actgacgcag catcacaaac agttctcgcg cgattttgat
     7381 gtagtcataa cgcacttcac ttagtgcaga gaaatttgcc atcccggtac caaaatcatc
     7441 cagccacagc gggccaaatt cacacatcga ggcaaaggtt gaatctttcg gcagacggat
     7501 atgctccacc agttcgaaac gcagccaggg aagacgctca atctggcgca ggatttttgg
     7561 ttgctgacgc agggcgatga gcgtagggcc atcaatattg accgatgcca gcaggccgtg
     7621 ctctacaaag aagtcggctt tttgcgccag caaatcaatc tgctctttca caacctccat
     7681 ccgatggctg acggtgattt cagtaaaata gcgatccggc ggcaggcgtt gcgaagggtt
     7741 caagggatgc gtgaccaccg ttaatagctc cacggccatt aaccgcccgc atgtttgata
     7801 gatcggctgc caggtgtaag cacgctcaca ctgcaaccaa aaacgccgtt cctgcaagct
     7861 ctcgatgctt gcttcagggt tgcttattcg ctggataacc tgccttatca tcaaagatgt
     7921 cctgtgttta aaagtgatgc tgcccggact cgacaaagat tatcggcgcg taagggcaga
     7981 actttaccgc ctggccgggg taaatttcct cactccacaa cggaatcttt caggaatacg
     8041 ttctggctca aaaaaataat ggaacactgt tttaatatgg ttgaccagca aaccaccaca
     8101 gcgcaaacta acgctaattt tttacagatc aggttcacga ctatgtccaa aaagattgcc
     8161 gtgattggcg aatgcatgat tgagctttcc gagaaaggcg cggacgttaa gcgcggtttc
     8221 ggcggcgata ccctgaacac ttccgtctat atcgcccgtc aggtcgatcc tgcggcatta
     8281 accgttcatt acgtaacggc gctgggaacg gacagtttta gccagcagat gctggacgcc
     8341 tggcacggcg agaacgttga tacttccctg acccaacgga tggaaaaccg tcttccgggc
     8401 ctttactaca ttgaaaccga cagcaccggc gaacgtacgt tctactactg gcgtaacgaa
     8461 gccgccgcca aattctggct ggcgagcgag cagtctgcgg cgatttgcga agagctggcg
     8521 aatttcgatt atctctacct gagcgggatt agcctggcga tcttaagccc gaccagccgc
     8581 gaaaagctgc tttccctgct gcgcgaatgc cgcgccaaag gcggaaaagt gattttcgac
     8641 aataactatc gtccgcgcct gtgggccagc aaagaagaga cacagcaggt gtaccagcaa
     8701 atgctggaat gcacggatat cgccttcctg acgctggacg acgaagacgc gctgtggggt
     8761 caacagccgg tggaagacgt cattgcgcgc acccataacg cgggcgtgaa agaagtggtg
     8821 gtgaaacgcg gggcggattc ttgcctggtg tccattgctg gtgaagcgtt agttgatgtt
     8881 ccggcggtga aactgccgaa agaaaaagtg atcgatacca ccgcagctgg cgactctttc
     8941 agcgccggtt atctggcggt acgtttgacg ggcggcagtg cggaaaacgc ggcgaaacgc
     9001 gggcacctga ccgcaagtac cgttattcag tatcgcggcg cgattatccc gcgtgaggcg
     9061 atgccagcgt aaacgcagca tatgcaaaag gctggataaa gtgttacccc tctatccagc
     9121 tcatccataa atacctgatg gttgtgctaa ctcatcacca tcaggcatta ctgtgcaggt
     9181 ggaatatccg tcacttcagg atgtacgtca tccgtggcga cagaggtggt cgcagcggcg
     9241 gtagatgggg ccatgatttg atcccaggcc gcctgcaacg ctttcatgtt aaattccggc
     9301 tcgcctttcg gctgcagcag tattaacgcc atatcattcg acaactgctg acgcagatcc
     9361 tgatttaaca tctccacagt caggctgtta aggaaatcct gccgcagttt ctgatactgc
     9421 tccggcgcga tatcgacgac ctgattttgc aacgaacgca tccgctggcc catcagaata
     9481 tcggtatcag cccgtgcata ggcggcaaac agtttctgca gctccagttt cttttgcgcc
     9541 actaacgcat tgaactcttc ttccggcaga cctttatcgc gaaccttcgc cagttcacgc
     9601 gccaccagat taaggttgct gttcagcttg tcgtttggtg attcgatgtt gatggcacac
     9661 tgcgcacgca gatacagcac acggcagtca aatccaagac cgatgtcttt gctgtcactg
     9721 gcgcttaacg cttgctgaac atgccagaac agcgcctcgc gggccaggtc cgcacgccag
     9781 tagcgcagca gtgcggctga ttcacgaatc ggctgccacg gcgtatccca catgatagat
     9841 aaccggtcct gacgcaccgc gtcagtcata atactcaccg cttccgcacg cagcggagaa
     9901 agcgtcggca ccggagctgg cgtttcacgt ttgcctttca gttcgccaaa cgttttgttg
     9961 atttggtcga caaccgagcg cgcatccacg tttcccacca ccagtagcgt cattgcatcc
    10021 ggggtgtacc atttctgata gaaatcttta attttttccg cttcaacggg ttgtttcagc
    10081 ggatcggcag gatcgtgacc taacaaggtt gaccctttca gacgatagcg ccaccagccc
    10141 tctttagtat cggcaggcca ggttgccacc atgtcctgac tttgcagcgc gtggttgatg
    10201 gtttctggtg tgatggtcaa tttgccagtg gcatttgcca gataagagag cgcttctttc
    10261 agcaaatcgt tacggttatt gggcaaactc agattaaaca gcgtggtgtc ataagagaca
    10321 attaccggcg gcatcgggcg tttagggtcg atcccctgct gccacaatga acgcgcctgt
    10381 gctgcgtcaa ggccaccgct ttgcgttagc gcaatacgag ggatggcgtg actgtaaccg
    10441 ctctgttgtg tactttcggc gagcgaaccg gtattaacca gcaggcgaat ttcaacacga
    10501 tcgctgggac gctggggggt ggtcagcact tgccactgta aaccgttgga aagcgtcccc
    10561 tgttgccatg ctggatcagg ctggagcgca tctgcctgca catagccagc agtggccatc
    10621 atcagcaaac cgcccgctaa aagtcgaatt tttatgccct gcatgtgaac ccctgatcaa
    10681 ctatcctggt aataaaaaaa ctgcccgttg tcggtcagtc taaatgacgt taaaaacact
    10741 tcgtgttaga ccgcaagaac atgaaaatgt cacggaagaa gtgaaataaa cctgaactca
    10801 cccaggtggt aagttcaggc acaggggtca attatgcgca aacacccgca ctcggggaag
    10861 ggagtgcggg cataagtggt gagattaaga ggataattcg tgcgttttgc catccggcgc
    10921 acgattattc agcacatcgt ccagtttttt gtggtccagt tctttcaccc acttagcaac
    10981 gacaatggtc gctacgccgt taccgaccag gttagttagc gcacgagctt ctgacataaa
    11041 gcggtcgata ccgaggatca gcgccagacc cgctaccggc aaatggccca ccgcagagag
    11101 cgtcgccgcc agcacgataa agccactacc cgttacccct gccgcccctt tagaagaaag
    11161 cagcaacacg attaacagcg tgatttggtg gacgatatcc atctgactgt tagtggcctg
    11221 ggcgataaac accgccgcca ttgtcaggta tatcgatgtg ccatcaaggt taaacgagta
    11281 gcctgtcggg atgaccagcc ccaccaccga tttacggcag ccgagtttct ccatcttgtc
    11341 gagcatacgc ggcagcgccg actcggaaga tgaagtcccc agtacaatca gcagttcttc
    11401 acggatgtag cggataaatt tgaagatact gaaaccagtc gctttagcga ttgaacccaa
    11461 taccagcacc acaaacagga tacaggtaat gtagaaacag ataatcagct gccccagttg
    11521 caccagtgtg ccgacgccgt atttaccgat ggtaaacgcc attgccccga gcgcaccaat
    11581 aggtgccaga cgcatgatca tattgatgat gccgaagatg acctgcgaga aactttcgat
    11641 cacgttaaaa atcagttggc ctttgctgcc cagacggtgg agcgcaaaac caaacagtac
    11701 ggcaaacagc agcacctgca gaatgttacc gctggcaaat gcgccaatga cgctcgccgg
    11761 gatgacatcc ataatgaagg cgacaatgcc ctggtctttc gcctgatcgg cgtaaaccgc
    11821 taccgctttc gcatcaagcg ttgccggatc gacgttcatt ccggcaccag gctgcacgac
    11881 gttaacgatg ataagaccaa taatcagcgc gatggtactg acaatttcaa agtaaagcag
    11941 tgcgactgcg ccggtacgac cgaccgcctt catgctttcc atgcccgcaa tgcccgttac
    12001 gacggtacaa aagatgacag gagcgatgat catcttaatg agcttaacga agccgtcgcc
    12061 aagcggtttc atttgctcgc ctatttcagg atagaaatgg ccaaggagaa taccaatggc
    12121 tatcgctgtc aggacctgaa agtaaaggct tttaaacaga gaggttttca tagggtgtcc
    12181 tttagtaaaa ccacaggtct t
//
LOCUS       DBBBSB010000115        12152 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.115, whole genome shotgun sequence.
ACCESSION   DBBBSB010000115 DBBBSB010000000
VERSION     DBBBSB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.115"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            126..995
                     /locus_tag="ACIUS0_003244"
     CDS             126..995
                     /locus_tag="ACIUS0_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGB0220872.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACIUS0_003245"
     CDS             complement(1198..1551)
                     /locus_tag="ACIUS0_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGB0220873.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACIUS0_003246"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACIUS0_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGB0220874.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACIUS0_003247"
     CDS             complement(3379..3672)
                     /locus_tag="ACIUS0_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGB0220875.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACIUS0_003248"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACIUS0_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGB0220876.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACIUS0_003249"
     CDS             complement(5220..5696)
                     /locus_tag="ACIUS0_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGB0220877.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACIUS0_003250"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACIUS0_003250"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGB0220878.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACIUS0_003251"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACIUS0_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGB0220879.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACIUS0_003252"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACIUS0_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGB0220880.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACIUS0_003253"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACIUS0_003253"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGB0220881.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACIUS0_003254"
     CDS             11052..11627
                     /locus_tag="ACIUS0_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0220882.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACIUS0_003255"
     tRNA            11734..11809
                     /locus_tag="ACIUS0_003255"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa ca
//
LOCUS       DBBBSB010000116        12114 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.116, whole genome shotgun sequence.
ACCESSION   DBBBSB010000116 DBBBSB010000000
VERSION     DBBBSB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.116"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..371
                     /locus_tag="ACIUS0_003256"
     CDS             <1..371
                     /locus_tag="ACIUS0_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220883.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACIUS0_003257"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="ACIUS0_003257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGB0220884.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACIUS0_003258"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="ACIUS0_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGB0220885.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACIUS0_003259"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="ACIUS0_003259"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGB0220886.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACIUS0_003260"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="ACIUS0_003260"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGB0220887.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACIUS0_003261"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="ACIUS0_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGB0220888.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACIUS0_003262"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="ACIUS0_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGB0220889.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACIUS0_003263"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="ACIUS0_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGB0220890.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACIUS0_003264"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="ACIUS0_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGB0220891.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACIUS0_003265"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="ACIUS0_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGB0220892.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACIUS0_003266"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="ACIUS0_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGB0220893.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACIUS0_003267"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="ACIUS0_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGB0220894.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="ACIUS0_003268"
     CDS             complement(11913..>12114)
                     /locus_tag="ACIUS0_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGB0220895.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2934 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DBBBSB010000117        12062 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.117, whole genome shotgun sequence.
ACCESSION   DBBBSB010000117 DBBBSB010000000
VERSION     DBBBSB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.117"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="ACIUS0_003269"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACIUS0_003269"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACIUS0_003270"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="ACIUS0_003270"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGB0220896.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACIUS0_003271"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="ACIUS0_003271"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGB0220897.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACIUS0_003272"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="ACIUS0_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="ACIUS0_003273"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="ACIUS0_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGB0220898.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="ACIUS0_003274"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="ACIUS0_003274"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGB0220899.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="ACIUS0_003275"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="ACIUS0_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGB0220900.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="ACIUS0_003276"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="ACIUS0_003276"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGB0220901.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="ACIUS0_003277"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="ACIUS0_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGB0220902.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /gene="agaW"
                     /locus_tag="ACIUS0_003278"
     CDS             9553..10332
                     /gene="agaW"
                     /locus_tag="ACIUS0_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGB0220903.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /gene="agaE"
                     /locus_tag="ACIUS0_003279"
     CDS             10322..11131
                     /gene="agaE"
                     /locus_tag="ACIUS0_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGB0220904.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /gene="agaF"
                     /locus_tag="ACIUS0_003280"
     CDS             11218..11652
                     /gene="agaF"
                     /locus_tag="ACIUS0_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGB0220905.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12062
                     /locus_tag="ACIUS0_003281"
     CDS             11649..>12062
                     /locus_tag="ACIUS0_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGB0220906.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3252 c         3077 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 cg
//
LOCUS       DBBBSB010000118        12003 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.118, whole genome shotgun sequence.
ACCESSION   DBBBSB010000118 DBBBSB010000000
VERSION     DBBBSB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.118"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..2252)
                     /locus_tag="ACIUS0_003282"
     CDS             complement(<1..2252)
                     /locus_tag="ACIUS0_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HGB0220907.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="ACIUS0_003283"
     CDS             complement(2276..2899)
                     /locus_tag="ACIUS0_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGB0220908.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="ACIUS0_003284"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="ACIUS0_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACIUS0_003285"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACIUS0_003285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGB0220909.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /gene="ubiG"
                     /locus_tag="ACIUS0_003286"
     CDS             7507..8229
                     /gene="ubiG"
                     /locus_tag="ACIUS0_003286"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGB0220910.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11932)
                     /gene="yfaL"
                     /locus_tag="ACIUS0_003287"
                     /pseudo
     CDS             complement(8357..11932)
                     /gene="yfaL"
                     /locus_tag="ACIUS0_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2781 a         3326 c         2956 g         2940 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggcgata gactggcccc
    11941 ctgaatctcc agacaaccag tatcacttat ttaagtgata gtcttaatac tagtttttag
    12001 act
//
LOCUS       DBBBSB010000119        11940 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.119, whole genome shotgun sequence.
ACCESSION   DBBBSB010000119 DBBBSB010000000
VERSION     DBBBSB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.119"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(175..915)
                     /gene="cysQ"
                     /locus_tag="ACIUS0_003288"
     CDS             complement(175..915)
                     /gene="cysQ"
                     /locus_tag="ACIUS0_003288"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGB0220911.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            1105..3048
                     /gene="cpdB"
                     /locus_tag="ACIUS0_003289"
     CDS             1105..3048
                     /gene="cpdB"
                     /locus_tag="ACIUS0_003289"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGB0220912.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3174..3554)
                     /locus_tag="ACIUS0_003290"
     CDS             complement(3174..3554)
                     /locus_tag="ACIUS0_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGB0220913.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3643..4503
                     /gene="qorB"
                     /locus_tag="ACIUS0_003291"
     CDS             3643..4503
                     /gene="qorB"
                     /locus_tag="ACIUS0_003291"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGB0220914.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4612..5577
                     /locus_tag="ACIUS0_003292"
     CDS             4612..5577
                     /locus_tag="ACIUS0_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGB0220915.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5685..6347
                     /gene="ytfE"
                     /locus_tag="ACIUS0_003293"
     CDS             5685..6347
                     /gene="ytfE"
                     /locus_tag="ACIUS0_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGB0220916.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6392..7804)
                     /gene="cycA"
                     /locus_tag="ACIUS0_003294"
                     /gene_synonym="dagA"
     CDS             complement(6392..7804)
                     /gene="cycA"
                     /locus_tag="ACIUS0_003294"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGB0220917.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8112..8732)
                     /gene="fklB"
                     /locus_tag="ACIUS0_003295"
     CDS             complement(8112..8732)
                     /gene="fklB"
                     /locus_tag="ACIUS0_003295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGB0220918.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8951..9496
                     /locus_tag="ACIUS0_003296"
     CDS             8951..9496
                     /locus_tag="ACIUS0_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HGB0220919.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9480..10127)
                     /locus_tag="ACIUS0_003297"
     CDS             complement(9480..10127)
                     /locus_tag="ACIUS0_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGB0220920.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            10339..11130
                     /locus_tag="ACIUS0_003298"
     CDS             10339..11130
                     /locus_tag="ACIUS0_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HGB0220921.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            11140..>11940
                     /locus_tag="ACIUS0_003299"
     CDS             11140..>11940
                     /locus_tag="ACIUS0_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGB0220922.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         3030 a         3090 c         2995 g         2825 t
ORIGIN      
        1 attaaatatc tcattataga agtaaactat caaaaaacgc tcattattaa aatgtaactt
       61 actagcaatt gaatttttgc actttaaata caatctcttt ttatttataa ctttttaggt
      121 tgggataaat caggatggca gaatcaggaa atacactgtt tctgccatct gaatttagta
      181 aatggacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat ccagcggttt
      241 accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc cggcggcggt
      301 gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt ccgccaccag
      361 gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct gttgcagata
      421 ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg ggcgcgcatc
      481 gcggacctga atcagcttgc gcacaccgca ctcttctttc caggccttgc cttctgccgc
      541 gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt tgccatggtc
      601 aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt tagtaccatc
      661 cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac cgggaggatc
      721 ttcttcagaa aggaccggaa tatccggtgc cagcgtacgt aaaccgtcca tgataacggt
      781 gtgagcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga cgacgtccat
      841 cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg caagctggca
      901 tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta ttgttttact
      961 tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc aaagcgtaaa
     1021 attgtggcat tcttcactgt tctataagta aggcgtttat tctccctttt ctttcgtatt
     1081 cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct ggccacgctg
     1141 attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac cactgatctg
     1201 catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa attcggactg
     1261 gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag cgtactggtc
     1321 gataacggcg atttgattca ggggagtccg ctggccgatt acatgtcggc gaaaggatta
     1381 aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta tacggtcggt
     1441 acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc gctggcggga
     1501 gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca gccaatgttt
     1561 acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa acagacgctg
     1621 aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa agctaattta
     1681 tccggaaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt gcctgaaatg
     1741 cgcgagaaag gtgccgatgt cattgtcgtt ctggcgcatt ccgggctgtc tgccgatccg
     1801 tataaagtaa tggcggaaaa ctcagtttat tacctcagtg aaattccggg cgttaacgcc
     1861 attatgtttg gccatgctca cgccgttttc ccgggtaaag attttgctga tatcgaaggg
     1921 gctgatatca ccaaaggcac gctgaatggt gttccggcgg taatgccggg catgtggggc
     1981 gatcatcttg gggtggtcga cttacaactc agtaatgaca gcggtaaatg gcaggtgacg
     2041 caggcgaaag cggaagcacg gccgatttac gacatcgcca ataaaaaatc cctcgcggcg
     2101 gaagacagca agctggtaga aacactcaaa gccgatcacg atgccacacg ccagttcgtc
     2161 agcaagccaa tcggtaaatc tgccgacaat atgtatagct atctggcgct ggtgcaggac
     2221 gatccgaccg tgcaagtcgt gaacaacgcg caaaaagcgt atgtcgaaca ttacattcag
     2281 ggcgatccgg atctggcaaa actgccggtg ctttcagctg ccgcaccgtt taaagttggt
     2341 ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa aaggccagtt gaccttccgt
     2401 aatgccgccg atctttatct ctaccccaat acgctgattg tagtgaaagc cagcggtaaa
     2461 gaggtgaaag agtggctgga gtgctccgcc ggacagttta accagattgc tcctaacagc
     2521 acgaaaccac agtcactcat caactgggat ggtttccgca cttataactt tgatgttatt
     2581 gatggtgtga attatcagat tgatgttacc caacccgccc gctatgacgg cgagtgccag
     2641 atgattaatg ccaatgcaga gcggattaag aacctgacct ttaatggcaa gccgattgat
     2701 ccgaacgcta tgttcctggt tgccaccaat aactatcgcg cttacggcgg caaatttgcc
     2761 ggtacgggcg acagccatat cgcttttgct tcaccggatg agaaccgctc ggtgctggca
     2821 gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc atccggcggc agataacaac
     2881 tggcgtttag caccgatagc tgccgataag aaactggata tccgtttcga aacttccccg
     2941 tcagataaag ccgcagcgtt tattaaagag aaagggcagt atccgatgaa taaagtcgcg
     3001 accgatgata tcgggtttgc gatttatcag gtggatttga gtaagtaaaa cacttctttt
     3061 tcggcctata aatcaaccgc atccggcatt tattggcgga tgcgatgctg ccgtatctta
     3121 tccaccctac aagccatgaa ccgtagaccg gataagcgca gcgcatccgg cagttatgcc
     3181 gcacgttcat cccgcaccgc caacacctcg ggcaaattca actcaatcca gtccgccagt
     3241 gcagcaacct tttcgctcac ctgctcgccc agcggcgtga ggctatattc cacatgcggc
     3301 ggcaccaccg gatacgcgat acggttaaga aaaccatcct gttctaacgc ctgtaacgac
     3361 tgcgcaagca tcttttcact caccccaccg attttgcgcc gcaggtcgct aaagcgatga
     3421 gtaccttcgc gtagcgccac cagaatcaac accccccaac ggctggtgac gtgtttcaac
     3481 acctcgcgcg acgggcactg ttccgcaaag agattaccct ctttcagttg ttgtgacagg
     3541 ctaacctgac tcatttcata cttacctttt tgcacgtact tactaaaagt aagtttaggt
     3601 gttagcgtat ttaaacacaa gacaaaacga tggagacttc ccatgatcgc tattactggt
     3661 gccactggcc aacttggtca ctatattatt gaatccttga tgaaaacggt tcctgccagc
     3721 caaatagtgg ctatcgttcg taatccggca aaagcccagg ccctggcagc acaaggcatt
     3781 accgtgcgtc aggctgacta cagcgatgaa gccgcactga catctgcact tcagggagtg
     3841 gaaaaactac tgctgatctc ttccagcgaa gtgggtcaac gtgccccgca gcatcgtaat
     3901 gttattaatg ccgcaaaggc ggctggcgtg aaatttatcg cttataccag cctgctacat
     3961 gcagatacct ccccgctcgg cctcgccgat gagcacatcg agacggagaa aatgctggct
     4021 gattctggca tcgtttacac cctgctgcgc aacggctggt acagcgaaaa ctacctcgcc
     4081 agcgccccgg cagcactgga acacggcgta tttatcggtg cggctggcga tggcaaaatc
     4141 gcctcagcaa cgcgggcaga ttatgcgaca gctgcggcac gcgtgattag cgaagccggt
     4201 cacgaaggca aggtttacga actggcgggc gatagtgcct ggacgttgac acagttagcg
     4261 gcagagctga ccaaacagag cggcaaacag gttacctatc aaaatctgag cgaagccgat
     4321 ttcgccgcgg cactgaaaag cgtcggactg cccgacggac tggcggatat gctggcggat
     4381 tctgacgttg gcgcatcgaa aggtggtctg tttgataaca gcaaaacgct tagcaaattg
     4441 attggctgcc caacgacaac gttagccgaa agcgtaagcc atctttttaa tgttaataac
     4501 tagttaatta aagtggcatc ctcccgcatc ctctctgata atgacgggat gccgggagca
     4561 atcatgtctg cttcctgaac gtttcttctg acagaccaat ggatgccagt aatgattagc
     4621 ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt cgggccgttg
     4681 atcgtgccgg aatacccggc gatgttgcag tcgatggggc gttatctggc gttagggtta
     4741 attgcgctac ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc gcgtcgggac
     4801 tggcttaccg ccttgatgct cactatgatg ggcaacctca tttattactt ctgccttgcc
     4861 agtgccattc aacgtactgg cgcgcctgtt tccacgatga ttatcggcac cctgccggtg
     4921 gtgattcctg tctttgccaa tctgctttat agccagcgcg acggcaaact cgcgtgggga
     4981 aaactcgccc cggcactgat ttgtattggc atcggcctgg cgagtgtgaa tattgctgag
     5041 ttaaaccacg gactccccga ttttgactgg gcacgttata cctctggcat cgtgctagcg
     5101 ttagtttccg tggtctgctg ggcatggtat gccctgcgca acgcccgctg gctgcgggaa
     5161 aatcccgaca aacatccgat gatgtgggcg acggcgcagg cgctggtcac gctgccggtt
     5221 tctctcatcg gctatctcgt cgcctgttac tggctgaata tacaaacgcc ggacttctcc
     5281 ttaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat agccgtgctt
     5341 tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc gacagtgatt
     5401 ctcgggccgc tgattgtttt cgaaacgctg gcaggtttgc tgtacacctt tttactccgc
     5461 cagcaaatgc cgccgctaat gacgctgagc ggtatcgcgc tgttagtgat tggcgtggtc
     5521 atcgcggtca gagcaaaacc agaaaaacct ttaactgaat ctgtctcaga aagttgacat
     5581 gctggcagtg agttaaataa gcctctgcta cgtaagggtt atagcttttg ccttaaaggt
     5641 gtatttaaaa tacatcttat cttattaaga atgaggtatc agctatggct tatcgcgacc
     5701 aacctttagg tgaactggcg ctctctatcc ctcgcgcttc ggctctgttt cgtaaatatg
     5761 atatggatta ctgctgtggc ggtaagcaga cgctggcgcg cgcggcggca cgtaaagaac
     5821 tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg attgagaaag
     5881 actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc taccacgatc
     5941 gtcaccgcga gcagctgccg gagctgatcc tgcaagcgac taaagtcgag cgcgttcacg
     6001 ccgacaaacc gagcgtgcca aaagggctga caaaatacct gaccatgctg catgaagagc
     6061 tttccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa caaggcatgg
     6121 gcagccaggc aatggggcca atcagcgtaa tggaaagcga gcacgatgaa gcgggcgaac
     6181 tgctggaagt gatcaaacac actaccaata acgtcacacc gccgccagaa gcctgcacca
     6241 cctggaaagc gatgtataac ggcattaatg aactgattga tgacctgatg gatcacatca
     6301 gtctggaaaa caatgtactg ttcccacgcg ccctagcggg tgagtgatct aaaagctgga
     6361 tggcattgcg ccatccagca tgataatgcg gttatttccg cagttcagca gcccgcttct
     6421 taccaataaa cagccagccc aaccccagcg cgataaacca cagcggggta accagcagcg
     6481 cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag aacgccatac
     6541 atacccagca catcagcttg ccgagcggca tcttgtagat cgacttttca tgcagatgag
     6601 gacgctgttt gcggtacacc aggtaagagc aaaggataat cgtccagacg aacataaaca
     6661 gaatcgcgga aacggttgta atcatcgtga acgcgccaat cacgctagga ttcacataca
     6721 acatcaccac gccgccgagc agacagatac acgagaaggt caaccctttc gcgggtactg
     6781 cgcgcttaga aagtttagcg aacgctttcg gcgcaacacc ttcctgcgcc agaccaaaca
     6841 gcatacggct ggtagagaag acaccgctgt tagcggaaga cgccgcagag gtcagcacca
     6901 caaagttgat cacgctggca gccgcaggca aacctaccaa cacaaacagc tcaacgaacg
     6961 ggcttttctc cgggactacc gaactccacg gtgtcacgga cataatcaca atcagcgcga
     7021 agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc agtgatttct
     7081 ccggatcttt ggtttccgca gctgttgtac ctaccagctc aatccccacg aaagcgaaaa
     7141 ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag ccgccgtcat
     7201 tccacaaatg cgcgaatgac gcttccacac cggtcggtga ctgaaagtgc atcgccacca
     7261 tgaccaggcc gacgacaatc agcgacacga tggcgacgat tttgatcatc gcaaaccaga
     7321 actccatctc accgaacatt ttcacggtgg caaggttgag cgtcagcagc agcactatca
     7381 ccgccagcga ggcgacccag tcggagagat cggggaacca gaactgagca taagccgtga
     7441 tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc cagccggtga
     7501 aatatcccgc ccacggcccc agtaaatcgg aagcgaagtc actaaaagat ttgtattcca
     7561 gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata aaaccaatga
     7621 tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgccg gaccccataa
     7681 acaacccagt accaatggca cctccaatgg caataagctg aacatgtcgg tttgtgagat
     7741 tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt acctgatcta
     7801 ccatgttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct ttagtcagtc
     7861 tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac gcgggatgaa
     7921 tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata tcacggtgat
     7981 aacgcgaaac agctcacaaa aatacacgct tacggggtct ttgcaacaca aaatagcgcc
     8041 gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc gaggggaaca
     8101 gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt gctgaacgga
     8161 gggatggatg cacctgcgcc gcgctcgcca tatgccagtt cctgcgggat agtcagttcc
     8221 catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat cacgccatta
     8281 accgggaatt cagcgggttc accacgagca acggagctgt caaacacggt gccgtcgatc
     8341 agtttaccgg tgtaatgaac acgaacgcgg tcggtacgtg ccggaattgc gccttcaccc
     8401 tggttgatca cgcggaattg caggccagat tcggtgctat tcacaccttc ttttttggcg
     8461 ttttcttcca ggtatttcac gccttcagca gccatcgcct ggaaacgctg acgacgaacg
     8521 gcatcggcgc gttcgtggat ttcacgcagc gcgcgatgca ccacatcaac cggcacagcc
     8581 ggatgtttgc cttccagcgc atcggcaata cctgcaacca gtgcttctgg cagcagccct
     8641 tccaggccag attcactcag ttgttgccct acctgcaaac caatgccgta gcttgcttgc
     8701 gcttcgatgg tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg gtataaaagt
     8761 agcgggcagc atatcagcca cacttctttg ggtaaaactt tgtcgcagga atgatgacaa
     8821 atcccgggga aaaagaaaca atagcaatat cccgtatgaa tataataaag gcgggtttac
     8881 cacggacatc atagcgttag cttattcata ggctatgatt gaggaacaag acgtggagca
     8941 ggaggaaacc atgcccgggc gctttgaact aaaaccaacc ctggagaaag tctggcacgc
     9001 gccggacaat tttcgcttca tggacccgct gccgccgatg catcgtcggg ggatcattat
     9061 tgccgccatt gtattggtgg tcggatttct gcttccatct gatgatacgc ccaacgcgtc
     9121 ggtggtgacg cgtgaagcgc agttagatat tcagtcgcaa tcacaaccgc cgacagaaga
     9181 acagccgcaa actacccaaa cgcaaccatt ccagccagat agcggaattg ataatcaatg
     9241 gcgctcttac cgtgtcgaac ccggtaaaac gatggcgcag ctgttccgcg accacggact
     9301 gcccgctacc gatgtctatg caatggcgca ggtagaaggc gcgggtaagc cgctgagtaa
     9361 tttgcaaaat gggcagatgg tgaaaatccg ccagaacgcc agcggcgtgg tgacgggctt
     9421 aaccattgat acggggaata atcaacaggt cctgtttacc cgccagccgg atggcagttt
     9481 tattcgtgcg cggtagatga agggtcaacc aaaagccagc taaggattgt ctctttgatg
     9541 accttacgtc tggcttccag tgcctggggg gaaaccatac tgatatgata aactaggcca
     9601 agcgtatgct ggttgatcaa ataatatccc ccaagcgcag caatattaat attcacctgc
     9661 aatgggtcga tacctggctt aaaaatattg tatttcttcc cttcatccaa taaggattcc
     9721 attaactgca agtgagcatg gttaatttct aagagacgct gtgattttgc ataatgcaca
     9781 cctttacttt gattttcact atgcacaatt tttaaaaacc aaggattagc aatataataa
     9841 tcccatgtaa aatcaataat tttctcaatg gctttttgtg gcggaagatc tgagaaatca
     9901 agggcctttt ctttttgtcg aatatcgttc caggtatatt ccagcacttc gataaataaa
     9961 ttttctttat taccaaagtg gtgataaacc gtctgtttac tacaccctgc tgcctttaca
    10021 atattatcta ctctggcccc ttcataacca tattcggcaa attcattaac cgcacatagc
    10081 agcaattttt ccttcaggct ctgtacgttg ctggtatcaa tatacataac gcttccccac
    10141 gacctcttcg tggtgactat cataacctga tttgatttca gcttcagaaa aaagggcaaa
    10201 aacttgtact ttcatcaggt tatcaaatgt cctgtgatcg ccatcgcata tcaaccaact
    10261 agatggttta tctttttatc ctccagtcgc aagctaatca tcaccaagga aacaaaattc
    10321 aaaaaagaga gctattgtat gaaatccact cgtcctgttg ccgtgattac cggagccact
    10381 cgcggtattg gaaagggctg tgcgcaggag cttgctcgtg gcgggtttaa cctgctgatt
    10441 aacgatcgtc ccgatgccga cagcgtagaa aaactgcata tcacacaaca agaatgtttg
    10501 gcggaaggcg tggaagtgat ttgcttccct gctgatgtcg gtgatctatc tctgcatgag
    10561 gagatgctcg acgcggcgca aaaccagtgg gggcggctgg actgtctgct caataacgcg
    10621 gggatttcag tgaaaaaacg cggtgacctc ctcgacctcg aaccagacag cttcgatcaa
    10681 aacattgcca ttaacacccg cgcgccgttc ttccttgctc aggcattcag taagcgctta
    10741 ctggcacagc cgaaaccgga agcggagctg ccgcaccgct cgattatttt cgtcagttcc
    10801 atcaacgcca tcatgctggc gatgaatcgc ggcgaataca ccattgccaa aaccgccgtt
    10861 tctgccgctg ctcgtctgtt tgctgctcgc ctttgtaatg aacaaattgg cgtttatgaa
    10921 gtgcgccctg gtctcatcaa aactgatatg actattccgg caacggctta ttacgacgaa
    10981 ctgattgcca aaggtctggt gccgtgggga cgttggggtt atccggctga tattgcttcc
    11041 accgtccgcg cgatggcgga aggtaaactg atttacacct gtggtcaggc ggtcgcaatc
    11101 gacggcggct taagtatgcc gcgcttctga ggctgaatca tgagagatct gttacaacgc
    11161 ccggatttat tcagtatcaa taccgcgaca ctgggctata aaacgccgct acccgccatt
    11221 attgatgcct gcgccgcacg cggtattggc gcaattgccc cctggcgcag ggagttgcag
    11281 ggtgaagatc tccagcaaat cacccgccag cttgccgcca gtaacatgag cgtttccggt
    11341 ttgtgccgta gcacttacta caccgcgccg acactggcgg aacgcaaact ggcgattgat
    11401 gataaccgcc gggcgctgga cgacgcagcg gtgcttaacg ccgcctgtta tatgcaggtg
    11461 gttggcggtc tgccaacggg cactaaagat ctatatgaag cgcgagaaca agtgaaacaa
    11521 gggattcgcc agttgctgcc acattcgaaa gatgtcggcg tgccaattgc gctggagcca
    11581 ctgcacccga tgacagctgc cgatcgctct tgcctgtgca cgttacgtca ggcgctggac
    11641 tggtgtgatg aactcgatcc cgatggtgag tttggcctcg gcgtggcggt ggatgtttat
    11701 cacgtctggt gggatccgga tctcgccagt cagatcctgc gtgccggaaa acgcatcctc
    11761 gcgtttcatg tttccgactg gttaatgccg accactgatc tggtcaatga ccgtggtatg
    11821 ccgggagatg gcgtcatcaa tattccgtca attcgtcggc tggttgaaaa cgccggattt
    11881 aatggtgcta ttgaactgga aatattctcg ccttactggt ggcagaaaga tattaacagc
//
LOCUS       DBBBSB010000120        11734 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.120, whole genome shotgun sequence.
ACCESSION   DBBBSB010000120 DBBBSB010000000
VERSION     DBBBSB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.120"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..851
                     /locus_tag="ACIUS0_003300"
     CDS             <1..851
                     /locus_tag="ACIUS0_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGB0220923.1"
                     /translation="RHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGFN
                     ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            873..2282
                     /locus_tag="ACIUS0_003301"
     CDS             873..2282
                     /locus_tag="ACIUS0_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220924.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="ACIUS0_003302"
     CDS             2260..3039
                     /locus_tag="ACIUS0_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGB0220925.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="ACIUS0_003303"
     CDS             3036..3896
                     /locus_tag="ACIUS0_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGB0220926.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="ACIUS0_003304"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="ACIUS0_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="ACIUS0_003305"
     CDS             complement(4636..4896)
                     /locus_tag="ACIUS0_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGB0220927.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="ACIUS0_003306"
     CDS             complement(4887..6158)
                     /locus_tag="ACIUS0_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGB0220928.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACIUS0_003307"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="ACIUS0_003307"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGB0220929.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="ACIUS0_003308"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="ACIUS0_003308"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGB0220930.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="ACIUS0_003309"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="ACIUS0_003309"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGB0220931.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="ACIUS0_003310"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="ACIUS0_003310"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGB0220932.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="ACIUS0_003311"
     CDS             11500..11652
                     /locus_tag="ACIUS0_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGB0220933.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2817 a         2988 c         3235 g         2694 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcttgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt ttta
//
LOCUS       DBBBSB010000121        11684 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.121, whole genome shotgun sequence.
ACCESSION   DBBBSB010000121 DBBBSB010000000
VERSION     DBBBSB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.121"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..348
                     /locus_tag="ACIUS0_003312"
     CDS             <1..348
                     /locus_tag="ACIUS0_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGB0220934.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(465..740)
                     /gene="yjfN"
                     /locus_tag="ACIUS0_003313"
     CDS             complement(465..740)
                     /gene="yjfN"
                     /locus_tag="ACIUS0_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGB0220935.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="ACIUS0_003314"
     CDS             complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="ACIUS0_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGB0220936.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1400..2149
                     /gene="yjfP"
                     /locus_tag="ACIUS0_003315"
     CDS             1400..2149
                     /gene="yjfP"
                     /locus_tag="ACIUS0_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGB0220937.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="ACIUS0_003316"
     CDS             complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="ACIUS0_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGB0220938.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="ACIUS0_003317"
     CDS             complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="ACIUS0_003317"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGB0220939.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4428..5825
                     /gene="ulaA"
                     /locus_tag="ACIUS0_003318"
     CDS             4428..5825
                     /gene="ulaA"
                     /locus_tag="ACIUS0_003318"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGB0220940.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5841..6146
                     /gene="ulaB"
                     /locus_tag="ACIUS0_003319"
     CDS             5841..6146
                     /gene="ulaB"
                     /locus_tag="ACIUS0_003319"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGB0220941.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6156..6620
                     /gene="ulaC"
                     /locus_tag="ACIUS0_003320"
     CDS             6156..6620
                     /gene="ulaC"
                     /locus_tag="ACIUS0_003320"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGB0220942.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6634..7284
                     /gene="ulaD"
                     /locus_tag="ACIUS0_003321"
     CDS             6634..7284
                     /gene="ulaD"
                     /locus_tag="ACIUS0_003321"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGB0220943.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7294..8148
                     /gene="ulaE"
                     /locus_tag="ACIUS0_003322"
     CDS             7294..8148
                     /gene="ulaE"
                     /locus_tag="ACIUS0_003322"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGB0220944.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8148..8834
                     /gene="ulaF"
                     /locus_tag="ACIUS0_003323"
     CDS             8148..8834
                     /gene="ulaF"
                     /locus_tag="ACIUS0_003323"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGB0220945.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8963..9238)
                     /gene="yjfY"
                     /locus_tag="ACIUS0_003324"
     CDS             complement(8963..9238)
                     /gene="yjfY"
                     /locus_tag="ACIUS0_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGB0220946.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9565..9960
                     /gene="rpsF"
                     /locus_tag="ACIUS0_003325"
     CDS             9565..9960
                     /gene="rpsF"
                     /locus_tag="ACIUS0_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGB0220947.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9967..10281
                     /gene="priB"
                     /locus_tag="ACIUS0_003326"
     CDS             9967..10281
                     /gene="priB"
                     /locus_tag="ACIUS0_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGB0220948.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10286..10513
                     /gene="rpsR"
                     /locus_tag="ACIUS0_003327"
     CDS             10286..10513
                     /gene="rpsR"
                     /locus_tag="ACIUS0_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGB0220949.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10555..11004
                     /gene="rplI"
                     /locus_tag="ACIUS0_003328"
     CDS             10555..11004
                     /gene="rplI"
                     /locus_tag="ACIUS0_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGB0220950.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11075..11638)
                     /locus_tag="ACIUS0_003329"
                     /pseudo
     CDS             complement(11075..11638)
                     /locus_tag="ACIUS0_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2872 c         3087 g         2958 t
ORIGIN      
        1 tccggcaata ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac
       61 gacttattgt cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg
      121 gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact
      181 catcagctat tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg
      241 gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag
      301 atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact
      361 gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg
      421 cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc
      481 gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat
      541 gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct
      601 gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg
      661 gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt
      721 gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca
      781 ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac
      841 gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta
      901 caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac
      961 gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg
     1021 agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg
     1081 gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc
     1141 gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt
     1201 acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg
     1261 aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc
     1321 aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc
     1381 agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt
     1441 tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca
     1501 cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg
     1561 tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc
     1621 gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt
     1681 acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc
     1741 gtcgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac
     1801 cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct
     1861 gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga
     1921 gtgggaagcg acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg
     1981 cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga
     2041 gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac
     2101 gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat
     2161 gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat
     2221 cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact
     2281 gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc
     2341 tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat
     2401 ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact
     2461 tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt
     2521 tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag
     2581 cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga
     2641 ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg
     2701 cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt
     2761 gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg
     2821 ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg
     2881 atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt
     2941 tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa
     3001 acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg
     3061 aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg
     3121 ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac
     3181 agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg
     3241 ataaccactt tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg
     3301 tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg
     3361 ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg
     3421 ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca
     3481 gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca
     3541 tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct
     3601 ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg
     3661 tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga
     3721 tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg
     3781 gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc
     3841 tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa
     3901 acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca
     3961 aatgtgccag gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc
     4021 gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt
     4081 taccttcatg atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact
     4141 attccttcag gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt
     4201 ttgagtgggg aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa
     4261 taattgtttc gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca
     4321 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
     4381 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
     4441 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
     4501 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
     4561 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
     4621 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg
     4681 atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag
     4741 gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta
     4801 ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg
     4861 ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc
     4921 tggtttcgct ctactggggc attacctcca acatgatgta caagccgact caggaagtga
     4981 cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata
     5041 aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct
     5101 ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg
     5161 gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact
     5221 ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc
     5281 agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc
     5341 tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg
     5401 ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg
     5461 tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg
     5521 ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc
     5581 tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga
     5641 gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct
     5701 tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct
     5761 tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg
     5821 cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag
     5881 gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc
     5941 acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca
     6001 tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg
     6061 gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag
     6121 cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg
     6181 agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg
     6241 gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg
     6301 atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg
     6361 gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc
     6421 tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg
     6481 atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag
     6541 aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc
     6601 gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg
     6661 ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc
     6721 gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat
     6781 ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc
     6841 aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt
     6901 gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg
     6961 cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt
     7021 attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt
     7081 gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc
     7141 ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc
     7201 gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt
     7261 tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc
     7321 atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa
     7381 acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc
     7441 cttgactgga gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg
     7501 cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac
     7561 gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt
     7621 ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa
     7681 acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag
     7741 gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag caaggcgctg
     7801 ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg
     7861 tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg
     7921 gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg
     7981 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
     8041 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat
     8101 tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa
     8161 aacagcaggt gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt
     8221 ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg
     8281 ttgcctacga aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg
     8341 tggaagggga gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt
     8401 acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg
     8461 cggggctggc gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc
     8521 cgtgtacgcg tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca
     8581 aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt
     8641 atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag
     8701 tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc
     8761 atatcgacag ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt
     8821 acgggcagaa gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa
     8881 ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat
     8941 gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt
     9001 tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt
     9061 cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca
     9121 cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg
     9181 cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag
     9241 atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt
     9301 taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact
     9361 gtgacggaat gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat
     9421 ttatttctgc tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt
     9481 cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct
     9541 gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct
     9601 gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca
     9661 gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac
     9721 aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat
     9781 gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc
     9841 aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt
     9901 cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa
     9961 tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag
    10021 gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag
    10081 gaagccggct ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa
    10141 aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt
    10201 tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa
    10261 ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg
    10321 tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta
    10381 catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca
    10441 gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga
    10501 tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa
    10561 gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa
    10621 gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa
    10681 aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg
    10741 gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct
    10801 aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct
    10861 gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg
    10921 cgtaccactg gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg
    10981 atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt
    11041 ggtcggcgtt ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac
    11101 cattctcttg tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc
    11161 ttatgtctcg gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat
    11221 ttgataagga acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca
    11281 atgcagcaat gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga
    11341 tataaagatc gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg
    11401 ttacaacgta tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa
    11461 caacctcgtg tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat
    11521 atttcagttc tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa
    11581 tattatctaa tgtagcttca tggttccatc caaaagaatt atcaccattc attgttactg
    11641 aatcgccacg ggtttaacag acacctcaga gtcatttaag atgg
//
LOCUS       DBBBSB010000122        11655 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.122, whole genome shotgun sequence.
ACCESSION   DBBBSB010000122 DBBBSB010000000
VERSION     DBBBSB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.122"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..794
                     /locus_tag="ACIUS0_003330"
     CDS             <1..794
                     /locus_tag="ACIUS0_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HGB0220951.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACIUS0_003331"
     CDS             complement(744..1937)
                     /locus_tag="ACIUS0_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0220952.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="ACIUS0_003332"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="ACIUS0_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGB0220953.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="ACIUS0_003333"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="ACIUS0_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGB0220954.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="ACIUS0_003334"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="ACIUS0_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGB0220955.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="ACIUS0_003335"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="ACIUS0_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HGB0220956.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="ACIUS0_003336"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="ACIUS0_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGB0220957.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="ACIUS0_003337"
     CDS             complement(10573..10716)
                     /locus_tag="ACIUS0_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220958.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="ACIUS0_003338"
     CDS             10955..>11655
                     /locus_tag="ACIUS0_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGB0220959.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DBBBSB010000123        11317 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.123, whole genome shotgun sequence.
ACCESSION   DBBBSB010000123 DBBBSB010000000
VERSION     DBBBSB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.123"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..233)
                     /locus_tag="ACIUS0_003339"
     CDS             complement(<1..233)
                     /locus_tag="ACIUS0_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220960.1"
                     /translation="MTQGWREDKCPSVRAGKKPRAGGAERGRAHAPPLSLRGRSDGGF
                     CLFRPVICLLLMMLLFLLLLLLLLLLLLLFFFFL"
     gene            complement(233..796)
                     /locus_tag="ACIUS0_003340"
     CDS             complement(233..796)
                     /locus_tag="ACIUS0_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HGB0220961.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQSVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(843..2204)
                     /locus_tag="ACIUS0_003341"
     CDS             complement(843..2204)
                     /locus_tag="ACIUS0_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HGB0220962.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2256..2486)
                     /locus_tag="ACIUS0_003342"
     CDS             complement(2256..2486)
                     /locus_tag="ACIUS0_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220963.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            3003..3251
                     /locus_tag="ACIUS0_003343"
     CDS             3003..3251
                     /locus_tag="ACIUS0_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220964.1"
                     /translation="MSRGPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATEAARPER
                     SLQARGFHASDRSGSPKPRQRVRRRLAGAPAAACRSRA"
     gene            complement(3524..3715)
                     /locus_tag="ACIUS0_003344"
     CDS             complement(3524..3715)
                     /locus_tag="ACIUS0_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220965.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3712..4134)
                     /locus_tag="ACIUS0_003345"
     CDS             complement(3712..4134)
                     /locus_tag="ACIUS0_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGB0220966.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4181..4606)
                     /locus_tag="ACIUS0_003346"
     CDS             complement(4181..4606)
                     /locus_tag="ACIUS0_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGB0220967.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5024..5800)
                     /locus_tag="ACIUS0_003347"
     CDS             complement(5024..5800)
                     /locus_tag="ACIUS0_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220968.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(5851..6285)
                     /locus_tag="ACIUS0_003348"
     CDS             complement(5851..6285)
                     /locus_tag="ACIUS0_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGB0220969.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6299..6520)
                     /locus_tag="ACIUS0_003349"
     CDS             complement(6299..6520)
                     /locus_tag="ACIUS0_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220970.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6521..7204)
                     /locus_tag="ACIUS0_003350"
     CDS             complement(6521..7204)
                     /locus_tag="ACIUS0_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGB0220971.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7281..7574)
                     /locus_tag="ACIUS0_003351"
     CDS             complement(7281..7574)
                     /locus_tag="ACIUS0_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220972.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7721..8683
                     /gene="parM"
                     /locus_tag="ACIUS0_003352"
                     /gene_synonym="stbA"
     CDS             7721..8683
                     /gene="parM"
                     /locus_tag="ACIUS0_003352"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGB0220973.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8686..9036
                     /locus_tag="ACIUS0_003353"
     CDS             8686..9036
                     /locus_tag="ACIUS0_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGB0220974.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9159..9440)
                     /locus_tag="ACIUS0_003354"
     CDS             complement(9159..9440)
                     /locus_tag="ACIUS0_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0220975.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9568..9802)
                     /locus_tag="ACIUS0_003355"
                     /pseudo
     CDS             complement(9568..9802)
                     /locus_tag="ACIUS0_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11251..>11317)
                     /locus_tag="ACIUS0_003356"
     CDS             complement(11251..>11317)
                     /locus_tag="ACIUS0_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0220976.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2749 a         3054 c         2806 g         2708 t
ORIGIN      
        1 agaaaaaaga agaacagtag cagtagcagt agcagtagca gtagcagtag caggaacaac
       61 aacatcatca gtaacagaca aatgacaggt ctgaaaagac agaagccccc atcagaacgt
      121 ccgcgtaatg aaaggggcgg cgcgtgagcg cggccccttt cagcgccacc ggcgcgcggt
      181 tttttcccgg cgcggacaga cgggcattta tcctcccgcc agccctgtgt catcaggcac
      241 gcaaacgaat aatcatcgtc gggacatcgg tataagcaaa cgtgccgcgc ggcagctcct
      301 cgcggacgtc agaaaacggt aacagcttct cctgctggcg tggcccgttg agacagacgg
      361 caaccagaac gccccccgga cgcaacaggg aaaaagcccg caggatatgc cggatatcct
      421 gcccgtgact gaacggcgga ttcataatga tccggctgta atactgcacc gactgccatt
      481 ccatgaagtc gccacactgt accctgacac cgttaaaatt ttcccgcaga taccggacca
      541 gcccgctgtt aatctccacc gcatcacaca tggcctccgg cgcggtatcc cgaatcgccc
      601 gcagtatggc cccggtgccg gcagagggtt ccagaatgtg atcacggttg ctgatttccg
      661 ccagcgtcac caggcgatca cagatcgggg caggactcac aaaaagctga gattcagccc
      721 ggatttcagc aaccggcgcg gcgttatcca gccttgcgcg catcagccgg atacgttctc
      781 ttaactgact gtgcatatca ttatctccgt catcccccgg ggatttcccg gggggatata
      841 ccttatttct gtgggatttc cgtgattttc atgtcggtga tatacacatt caccagacgg
      901 aaattattgt ccattgtgcg gcggtagcgg tatgccccgt ggtcttctgc ttcttccaca
      961 ctgcgcacgg ccttacagtc ccggggcagg gcggcccact gcgcctttgt catttcccgg
     1021 aaaccttccc ccggatagtt gactaccggc ggacgcttcg cggcctgact ggcgacggca
     1081 gcctcttctg ccgatggtgc tttgtagtcc gtgatgcgat cgggcgtcac tttcatcgtg
     1141 ccgctgtacc cgagaaaact gtaatttggc gttgtgactg aactcaccgc cccgttgctt
     1201 ttgttcacgc ggatgatagt cagccactcg ccccggctga aaacctgtcc gcccggctca
     1261 aaatcctgtg tccgggtaac cacgccgccg ctttcgtcca gcatggcacg ctcatagatc
     1321 aggcggttct gatagtggtt aacccagcgt tgctgatgct gaatctgccg ttcatgacag
     1381 cggatcgcaa tttcgcgggc ctgctccgtg gtgatgatgt catcatccag ggccgaccat
     1441 aacgaccggc tcccttcata ctggcttttt tctgccgggc gcggatacgt atccagcggg
     1501 aaacaggcgc tgatatggtc atgactgctg atgagttttg ccatattcag atccagcgat
     1561 tcagcccgcc acatcgtcag atatttctgc gactgtgcga tggtcttttc agccttgcgc
     1621 aaatcagctt cgattttttt gatacgacgc cagcgaacgt ccgggcgttc tttatacttc
     1681 gcgtgaagca gtgccgaccg cgcccgttct tcccagtatt ccgcacgttc aaagagcatc
     1741 acggcacgtt tcatgccgtt ttcaatacgc tgcgcatcac gacgggcgcg gcgttcgctg
     1801 tgatgcccca caagaatggg ctgaccgggc gggatcatcg cggccagtct ttccacttca
     1861 tcaagtgcct gtgcggattc actggcccgc tttccgctgt atccggtaaa ccgctccgcc
     1921 cgtgcttcct gacgttcagc gagcgtgctg tcttcatcct caatctctcc ggcaagtgag
     1981 aggagcacgt cttcccggcc cggtgtccag gctggcgcga caaacagcgc ctgtctgggt
     2041 gcccagcgga aaccggcatc atgcactttt ttatacgtct ccgggtcaag acgtgacgcg
     2101 gcatacaggc gcagtttgtt gtcatccggt gagtaggtcg cgcggtacat ctgcacaggg
     2161 ctggcggctt ccggggcggt catggtgatt gcagctaatg tcataaaatg ttctccttcc
     2221 gtctgttgcc gcccctcgcg gggcggctca cctcatcagt taaaagcgcg tttcagttcg
     2281 agtgtgcgga gcacctgccc gaaagccgta tttttcatgg cggggatgtg tacaccacgt
     2341 ccccggtgaa aactccgggc aatgtgtcgc ccgaaggctt tcagcacggg gtcaatttcc
     2401 ggcgcgggga tgtcttcgcg tctgaaacgg ggatgacggc ggggctttac agccgctgcg
     2461 gaaggtgaaa caatacttac cgtcataaat gaaactcctt atgactgttg aatggaaccg
     2521 cagaaactgc tcgaacagtt tttgcggtgt ggattaagca ggtcctcccc ggcctgcttt
     2581 tttcatatct gcgtctgacg ttctccggtg ttttcctcct tctgttcctg cgttctcatt
     2641 acctgccagc cggatgactg acagaacaaa taaatgcgta ttgccggagc ctgaaataaa
     2701 tcctgatttt taaagaacat cacccgttga gtttcgggag ccgcggtttt gacgtttcgc
     2761 gggaacctgc tgctgtattc cctgtggtat gaacttcacc cgtttgagaa ccttcgccgc
     2821 aaggggcatc actgccacgg cctgacgcac ctgtcctggg cagaacgcaa aattttttac
     2881 cggtttttgg gtaaaaaatt ttgtgggctg ttcatttacc ctggacagtc agtgcggcag
     2941 gttgtggctg tctatgcccc cggcgacagg gactcatacg ggtgtgtggc acccggcagg
     3001 caatgagcag gggcccgccg caacgacgga gagcgcgtgc gcgaaacggc gttgcccctt
     3061 acatcccggc ggccttttgc cgggatgccg ggagcgcagg ccacggcgcg acagaggcgg
     3121 cgcgaccgga acggtcgctg caagccaggg gttttcacgc atcggaccgt agcggcagcc
     3181 cgaaaccgcg ccagcgggtt cggcggagac tggcaggtgc gccagcagct gcctgtcgga
     3241 gtcgggcctg acgaccggat gccgccaggc agccgggagc cgaaggtgcg cccggcagac
     3301 agaacaacac tgcagtagtc agaatcagag cagtagataa aggaacaaca acatcaggaa
     3361 cagttccata acataagaaa gaaacactcc tgagtcagac acatcacaga cgggaagggt
     3421 aacgggcgac gcgccagcgt ggcccgcttc ccttccggca ggcgtcagcc tgccaggggt
     3481 tttcgcgcgg gctgacaggc ggcctccacc gcctgcccct gtatcacctg tttatgctgc
     3541 cctgaaggat gtagtgaccg ccgaggaata cagcctgctg attcgccaca aaatcgcaga
     3601 tttcatcaac aatctgcgca gtggtgagcg gtttctgccc catttcacga cgccgcgcat
     3661 taatcacggc tttgtaattg cgcagaattt cgcgggtttc tgtgctgata ttcatgacat
     3721 gtgctccccg tccagcgcct gtaacacaac gtccattgct tcgcgttctt ccctgacgaa
     3781 tgcgtcaccg tcgcccccgt tctcgctccc gacggcatac aggcgcttca tttcactgcc
     3841 tgccagcgcc atcacttttt ccagcatcac cgccggaact gtcacctgac ggctggcgcg
     3901 cttttcttcc atcagctcac gaacaacgtc gtgaacaatg ctgacatccg caccatgcgc
     3961 aaaggcatcg tccagtcgct gcatgacggt ttccagctca tcgtctgtca gactccagtc
     4021 ctgggctatg tgacgcacat ccccacgggt cagcaccacg gcaaaaacgc attcactgtc
     4081 cggcacgtct gtgttcagca cttcgcggat aatttcttta acagtacagt acatgttctc
     4141 gtctccttta caggggcggc attgccgccc cgtccgttaa tcagtccagt gccgcgtaaa
     4201 tcgcgttgca ttcggggtga aattcgatgt gacgccacaa ctgcgcatcg cgcatccggt
     4261 aaagctgggt cagtcctgca tccccgctgt cgtggtacag ccacaactgg cggttaatca
     4321 caagggaagt cagaatgatg cctgcggcat cagcactcac ggtacggtca aaccagtttg
     4381 caccgttcac catgtggaaa cggtcgccat ccggcatcat gtaaccgcca ccgtccggca
     4441 ggcggatgaa gtgccagaag cctccctgat attccggcat cattttttgc gccagcgcgt
     4501 acacatacgc ctcggcatac agaaaatcgt tgccaaacag atacggtaga aaggagagac
     4561 ggtcgcactc ttcaacgttc agagtgacag gtttcgcata ttgcatgatg ttttcctccg
     4621 ttgttcacat attccgcgac accgggcggt gccgtgcctc cataaaagcc gggcgataag
     4681 gcggggatgc acagggcgca agggaagccg cagcctgacg cacacggctg gcgggagttg
     4741 gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga aaatcacggc agacgggaag
     4801 gagggaccgg gtgaccgcag cggcgaagcg tacccttgca tccctgccag cccggctacg
     4861 atggaggcac ggcacagccc gcacacgacg ctgaggccac ataactgaaa acccacaagg
     4921 ccatgccccg catggccggg taaagcggag ccggcggcga cttcctttgt gctctgtccg
     4981 gggaccggca ggaaaaaggc ggggatttac cccgccagtg tcatcatttc cggatttttg
     5041 ccagggcatc cgccatgctg cggatttcgg cccgggcttc ccggaccacc tcccgcagaa
     5101 caccacggtc aaaccagcgg cggggcggtt catccggtat acgcagcaca tcgcaggcca
     5161 gcccactgga caggatatgc tcaatacaat cgctgaccag gtacatttcc accgacgaaa
     5221 aacgtgctcc ccggttcacg atggcatcga gcaccacggg caggttttcg tcatcttcat
     5281 cgagccagca ggggtgatcg ctttccttca caaccagacg aaagcggcgc gtctggcgag
     5341 tcagcaacgt ctgaccggtg gtggtcagcg ccgcccggat acgccgcgca tcttctgcca
     5401 cccggcaact gacactgcgt accgcatcgc gcaccgccgc aagtacagcc ctgatttcgt
     5461 cttcggtcag ccattctccg gcatacggga aagacacctg cgttccgtct gccggaagta
     5521 ccggcagcga ctgccactga ctgccggaca gtaccagttc cgccccggtt ccggtcagtt
     5581 tctgaatggc ggaggccagg agcggcggca gtacgccgcc gtgacggttg ataagtaacg
     5641 tcgccagccg gggcgcatcc agcagaacat aaatgctgat ccagcccgga tacttcgggt
     5701 tacgctggtc aacatccgtc tcttccggcc agccctgcgc cagcagcagg ttgcgggcgt
     5761 gacgacaaat cagtgcgttc agcgtgctgt tttcgttcat gattaagcgt tctccctgta
     5821 tcgccgcccg cagggcaggc ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag
     5881 cagggtgcgg gcctgattaa ccacctcctg acggcgggtg acgcattccg ggacggcgag
     5941 gccatgatcc cgggcggccc attcacgttt ccacaatgcc tgttcagcgg tctgaatcag
     6001 ggacgccagc agttcagcag ggacggtcac caggcggttt tcgctctcgt tgcggataat
     6061 ctccatcacc gcagcggaag aaataccgga ctcaatccgc tggtcttccg ggatgtcctc
     6121 caggcgtgcc aggacggtcc tgatttcatg accggtcaga gaaatttcca taccgtcagc
     6181 cagtgcctga atctcttccg gggaccagat aaccagcatc agcggcatgt ctcccggata
     6241 ctttactgcc agctcccggc atagcgtttc gcatgttccg tacattctcg tctccttctc
     6301 aatctttaaa gcacgcttcc agttccagaa tttcggtata tgcctccatc aggaggcggt
     6361 ttgcgcgaaa cagcgtctga cgcaccagac gttccggcag ggtgtcggca ttaaagctgt
     6421 accgctgctc catctcattc atggttgtac gcaggtcgca catggcattg gccacctccg
     6481 ccacttcggc gcgcagtttt tcgtgtcctg catagttcat ttacgcagcc tccggcataa
     6541 accagtcatc attcgcggcc ccctgctgca tggcccgttg cacggcggca aggcgttgct
     6601 gcccggcacg gtgatactgc tcaagcagct cgataccgat ataccggcgt ccggactgga
     6661 gggcggcgac gcaggttgag ccgctgcctg caaacgggtc cagcacaatt gcgttcgggt
     6721 gtgtgaagct ctcaatcagc ggttgcaggc tggtaacggg cttttccgtc gggtgatggc
     6781 gattgcccga atatttccag cccagcacgt ccggcagcgg gttttgtggc agacgtggac
     6841 ggccttttgc caggatgtag gcacattcgt ggcgatagcc cacatatgcg gccttcgatg
     6901 tgtagttttt ggtgaagacc aggtgaccaa caacgctgaa tcccgcattt ttccaggcgg
     6961 ccataaagcg atcgacgcgg ttccagccat agaagctcac cattaacgcg tcttttttga
     7021 gtacgcggta catttcatta caggctggtt gcagccattc atcggtttta tcgccggcga
     7081 tggtgcgccc ggaacggtca cggaaaccga cgagatacgg cgggtcggtg aggatgaaat
     7141 cgacagcatt gcccggaaac gtggccataa cacgcacgca gtcacccagg ataaaacggg
     7201 acataacagc actccttctc ttacagaggg caccgggagg gcacccctgt ggtgcccgtt
     7261 ccccgctgcc tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac
     7321 cgcagccgtc gtctttttga cggcgaggat atgccgcgaa agcggcttga agggcgttca
     7381 tcagaaaagg gcgacgggct ggcgtacact gtcaccaacg ccagccggga gcggtaaaag
     7441 cgcccgtccc gtgcccggcc ggacgcggga cactgaccac aggcagcgtt cctgccgcgc
     7501 cgcgtcagcc ggcgcagtaa aaagaggagc ccggcgacat cttttgttac ccgccaaaca
     7561 aaacccaaaa acaacccata cccaacccaa taaaacacca aaacaagaca aataatcatt
     7621 gattgatggt tgaaatgggg taaacttgac aaacaaaccc acttaaaacc caaaacatac
     7681 ccaaacacac accaaaaaaa caccataagg agttttataa atgttggtat tcattgatga
     7741 cggttcaaca aacatcaaac tacagtggca ggaaagcgac ggaacaatta aacagcacat
     7801 tagcccgaac agcttcaaac gcgagtgggc agtctctttt ggtgataaaa aggtctttaa
     7861 ctacacactg aacggcgaac agtattcatt tgatccaatc agcccggatg ctgtagtcac
     7921 aaccaatatc gcatggcaat acagcgacgt taatgtcgtt gcagtgcatc acgccttact
     7981 gaccagtggt ctgccggtaa gcgaagtgga tattgtttgc acacttcctc tgacagagta
     8041 ttacgacaga aataaccaac ccaatacgga aaatattgag cgtaagaaag caaacttccg
     8101 gaaaaaaatt acattaaatg gcggggatac attcacaata aaagatgtaa aagtcatgcc
     8161 tgaatctata ccggcaggtt atgaagttct acaagaactg gatgagttag attctttatt
     8221 aattatagat ctcgggggca ccacattaga tatttctcag gtaatgggga aattatcggg
     8281 gatcagtaaa atatacggag actcatctct tggtgtctct ctggttacat ctgcagtaaa
     8341 agatgccctt tctcttgcga gaacaaaagg aagtagctat cttgctgacg atataatcat
     8401 tcacagaaaa gataataact atctgaagca acgaattaat gatgagaaca aaatatcaat
     8461 agtcaccgaa gcaatgaatg aagcacttcg taaacttgag caacgtgtat taaatacgct
     8521 caatgaattt tctggttata ctcatgttat ggttataggc ggtggcgcag aattaatatg
     8581 cgatgcagta aaaaaacaca cacagattcg tgatgaacgt tttttcaaaa ccaataactc
     8641 tcaatatgat ttagttaacg gtatgtatct cataggtaat taatgatgga caagcgcaga
     8701 accattgcct tcaaactaaa tccagatgta aatcaaacag ataaaattgt ttgtgataca
     8761 ctggacagta tcccgcaagg ggaacgaagc cgccttaacc gggccgcact gacggcaggt
     8821 ctggccttat acagacaaga tccccggacc cctttccttt tatgtgagct gctgacgaaa
     8881 gaaaccacat tttcagatat cgtgaatata ttgagatcgc tatttccaaa agagatggcc
     8941 gattttaatt cttcaatagt cactcaatcc tcttcacaac aagagcaaaa aagtgatgaa
     9001 gagaccaaaa aaaatgcgat gaagctaata aattaattca attattattg agttcccttt
     9061 atccactatc aggctggata aagggaactc aatcaagtta ttttcttacc agtcattaca
     9121 taatcgttat tatgaaataa tcgtttgcac tgtctctgtt attcaggcaa tttcaataaa
     9181 ggcacttgct cacgctctgt cattttctga aactcttcat gctgcatttc gatttttctt
     9241 ctaaaatcat aacttgcata cctgttcttt ttatgtatat ccagatctat ttttgtgacg
     9301 agttcaaacc acaatgatag cactccccat gcagcgttat gaaacatttc cgcaacagat
     9361 ttagagtctg tattaaccgc ttcagtcatg tagtcgctaa tacgttctgc agtgattttc
     9421 gttatttcat cgtaattcat tttatcccct acaagactat ttaatggaat gaaaaatatt
     9481 tggtctgata acaatgctcc tgaattaaca cctgcaggct atgttaaagt tgccctcaca
     9541 cacaatctac ctgttttctc gtgtgtgtca caggcgcaat acataaatag tctggccatt
     9601 aagttcgtaa cggatttcgt actcgccagc aaaaatccgt ctgacatccc tggtttcaaa
     9661 ctgaaacaac tgttcatccc atacgtggat gagttaacag aatgaccggc gcctgtgtca
     9721 gggactacac cgttttggct gcagctggtt tactgaccaa cgccagaaaa tcatataact
     9781 gcgtcaatgt cagcgtatcc agcgcgactg tattattttt ctctgcaacc agaatattgc
     9841 attaggttat taccaggtgt tctaatgtac aggtaaagac gttctttata atctgaaatc
     9901 tctactttta atccctcaga ctttgcctgc tgaaagccct cgtcaaggtg cgcttttatt
     9961 aggttaatgt ctggatgtct tgtatcgggg gcaattctgt attggtgtaa cgatggggtg
    10021 gatgttccct gcaataagac ctccagatag agattggcac aaccattccc attatttcta
    10081 atagtttatt ggtaatcaca aactgtatat ttagccatta tattgaatcc tgtgaagtaa
    10141 acggcgacgg ctcaaaattt tatcaaacct aagataattc taccatcaaa gttaataatt
    10201 tgaatttata tgacggctct atgggggggt aaaaatcccc gtgcagagag gcgattttca
    10261 gagttgaaaa cgtcgtcgtt aagaacacca aagtcagtac caccaacccg gaaagtagct
    10321 tcatgcaccg ggacaacagc agttttggtg tccgaaatcg tacaattctg tacatctacg
    10381 caaggaaatg tacggaagta agccaaatgg ttatcggacg gtcggaacat ttcgtacagc
    10441 ctttacgttc agtgagttag ctggcctaga tgtaaccact cgttcaactc aattctctta
    10501 gcgtgggaaa atttccgttt tccaagcggc cccctcaagc ggacccctta tagtacaata
    10561 attttcgata tccaaactgc ccccaattac atctccagtc tctggcagca gagatcatat
    10621 atttccattg ccgccgtagc cacgatattc tggctattca caaaaacaat ttagccccaa
    10681 agcgcagtaa aaaagtacac tatgaaggtg tatgatacct tcatagtgct taagtaatta
    10741 tgagtaatat aatctgtgta aactactaat tgcttcaccg tcagtgatta aatgcatagc
    10801 tgttccttgt cttatataat acctattttc aaaatgacag acttctccga cactttttat
    10861 ttcgattttt aaaacatgca gtccttcata tgaacataat gaaatatctt ttagtatttg
    10921 ttgtttcaaa gcctcattaa aattaaatga ttctattttt tgttttatga acagaaagta
    10981 attatctatg ttttttgatt gaattacagc ttcatgatca attccattga tataatagcc
    11041 attgcactca ataggggtaa caccatataa agtcttaact ctgtctgccg tagctttggt
    11101 atcggaaaca ccaactaaaa tataaccatt agattctcta ccgatgttat ttatggcgac
    11161 agccgtttgg actatattaa tgaaacattc ttcgtctatt ttattctcgc ctgagagaat
    11221 aaataatccc tgtttggtaa tgactccaac ttattgatag tgttttatgt tcagataatg
    11281 cccgatgact ttgtcatgca gctccaccga ttttgag
//
LOCUS       DBBBSB010000124        11184 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.124, whole genome shotgun sequence.
ACCESSION   DBBBSB010000124 DBBBSB010000000
VERSION     DBBBSB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.124"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACIUS0_003357"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="ACIUS0_003357"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGB0220977.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACIUS0_003358"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="ACIUS0_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGB0220978.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="ACIUS0_003359"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="ACIUS0_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGB0220979.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="ACIUS0_003360"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="ACIUS0_003360"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGB0220980.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="ACIUS0_003361"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="ACIUS0_003361"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGB0220981.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="ACIUS0_003362"
     CDS             complement(5839..6879)
                     /locus_tag="ACIUS0_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGB0220982.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="ACIUS0_003363"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="ACIUS0_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGB0220983.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="ACIUS0_003364"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="ACIUS0_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGB0220984.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="ACIUS0_003365"
     CDS             7821..8261
                     /locus_tag="ACIUS0_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGB0220985.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="ACIUS0_003366"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="ACIUS0_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGB0220986.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="ACIUS0_003367"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="ACIUS0_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGB0220987.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11184
                     /gene="rsxC"
                     /locus_tag="ACIUS0_003368"
     CDS             9490..>11184
                     /gene="rsxC"
                     /locus_tag="ACIUS0_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGB0220988.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2598 a         2877 c         2817 g         2892 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgc
//
LOCUS       DBBBSB010000125        11095 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.125, whole genome shotgun sequence.
ACCESSION   DBBBSB010000125 DBBBSB010000000
VERSION     DBBBSB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.125"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            459..1859
                     /locus_tag="ACIUS0_003369"
     CDS             459..1859
                     /locus_tag="ACIUS0_003369"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGB0220989.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            2015..3550
                     /gene="gadC"
                     /locus_tag="ACIUS0_003370"
     CDS             2015..3550
                     /gene="gadC"
                     /locus_tag="ACIUS0_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGB0220990.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3681..5000
                     /locus_tag="ACIUS0_003371"
     CDS             3681..5000
                     /locus_tag="ACIUS0_003371"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGB0220991.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5377..6447
                     /gene="dosC"
                     /locus_tag="ACIUS0_003372"
                     /pseudo
     CDS             5377..6447
                     /gene="dosC"
                     /locus_tag="ACIUS0_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            6447..7064
                     /locus_tag="ACIUS0_003373"
                     /pseudo
     CDS             6447..7064
                     /locus_tag="ACIUS0_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            7322..7654
                     /gene="ddpX"
                     /locus_tag="ACIUS0_003374"
                     /pseudo
     CDS             7322..7654
                     /gene="ddpX"
                     /locus_tag="ACIUS0_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7668..8564
                     /gene="ddpC"
                     /locus_tag="ACIUS0_003375"
     CDS             7668..8564
                     /gene="ddpC"
                     /locus_tag="ACIUS0_003375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGB0220992.1"
                     /translation="MMLSEETSAVRPQKQTRFNGANLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8561..9535
                     /locus_tag="ACIUS0_003376"
     CDS             8561..9535
                     /locus_tag="ACIUS0_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGB0220993.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9528..10454
                     /locus_tag="ACIUS0_003377"
                     /pseudo
     CDS             9528..10454
                     /locus_tag="ACIUS0_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(10510..10941)
                     /gene="osmC"
                     /locus_tag="ACIUS0_003378"
     CDS             complement(10510..10941)
                     /gene="osmC"
                     /locus_tag="ACIUS0_003378"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGB0220994.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2745 a         2712 c         2757 g         2881 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
      781 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
      841 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
      901 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
      961 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
     1021 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
     1081 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
     1141 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
     1201 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
     1261 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
     1321 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
     1381 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
     1441 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
     1501 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
     1561 ccggtcgccc ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc
     1621 cgggatacac cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg
     1681 ccttcactct cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg
     1741 gcttcgaaat ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc
     1801 tcagcgatca cccgaaactg cagggtattg cccagcagaa cagctttaaa catacctgat
     1861 aacgtttaac ggtaacggtg tcccgaaacg aacccgtttc gggataattt ccaaagtctg
     1921 ttcactggca ttagcaacgg aaaatattgt tctgaatacg cttcagaaca aaacaggtgc
     1981 ggttccgaca ggaataccgt tttaggggga taatatggct acatcagtac agacaggtaa
     2041 agctaagcag ctcacattac tcggattctt tgccataacg gcatcgatgg taatggctgt
     2101 ttatgaatac cctaccttcg caacatcggg cttttcatta gtcttcttcc tgctattagg
     2161 cgggatttta tggtttattc ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg
     2221 ctgggaagaa ggtggtgtct tcgcctgggt atcaaatact ctgggtccga gatggggatt
     2281 tgcagcgatc tcatttggct atctgcaaat cgccattggt tttattccga tgctctattt
     2341 cgtgttaggg gcactctcct acatcctgaa atggccagcg ctgaatgaag accccattac
     2401 caaaactatt gcagcactca tcattctttg ggcgctggca ttaacgcagt ttggtggcac
     2461 gaaatacacg gcgcgaattg ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt
     2521 tattttgatc gcattagcgg ctatttacct gcactccggt gcccccgttg ctatcgaaat
     2581 ggattcgaag accttcttcc ctgacttctc taaagtgggc acactggttg tatttgttgc
     2641 cttcattttg agttatatgg gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa
     2701 ccccgggcgc gactatccac tggctatgtt actgctgatg gtggcggcaa tctgcttaag
     2761 ctctgttggc ggtttgtcta ttgcgatggt cattccgggt aatgaaatca acctctccgc
     2821 aggggtaatg caaaccttta ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac
     2881 ggttcgcgtg atctccgcac tgctgttgct gggtgttctg gcggaaatcg cctcctggat
     2941 tgttggtcct tctcgcggga tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt
     3001 cgctaaaatg aacaaaaatg gcgtaccggt aacgctggtc atttcgcagc tggtgattac
     3061 ttctatcgcg ttgatcatcc tcaccaatac cggtggcggt aacaacatgt ccttcctgat
     3121 cgcactggcg ctgacggtgg tgatttatct gtgtgcttat ttcatgctgt ttattggcta
     3181 cattgtgttg gttcttaaac atcctgactt aaaacgcaca tttaatatcc ctggtggtaa
     3241 aggggtgaaa ctggtcgtgg caattgtcgg tctgctgact tcaattatgg cgtttattgt
     3301 ttccttcctg ccgccggata acatccaggg tgattctacc gatatgtatg ttgaattact
     3361 ggttgttagt ttcctggtgg tacttgccct gccctttatt ctctatgctg ttcatgatcg
     3421 taaaggcaaa gcgaataccg gcgtcactct ggagccaatc aacagtcaga acgcaccaaa
     3481 aggtcacttc ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa
     3541 gaaacactaa ccatgtctga atggttataa gacaagggag cgataattca tcgctccctt
     3601 tttcgtgctt gctgtcgtat tgacttaacc gggataagta cgagaatgag cgcacatctg
     3661 tttaccggaa accagcacat atggatatct gctcccgaaa caagaaatta acgattagaa
     3721 gaccagcgat actggttgca ttggcacttt tactgtgtag ttgtaaaagc acgcctccag
     3781 agtccatggt gacaccacca gcaggttcaa agccaccagc cacgacgcaa caatcgtcac
     3841 aaccgatgcg tggcatctgg ctggccacgg tttctcgcct cgactggcca ccggtttcct
     3901 cggttaacat tagtaatccc accagccgag cccgtgtaca acaacaggcg atgatcgaca
     3961 aactggatca tctccaacgt ctcggcataa acacggtctt tttccaggtc aagccggacg
     4021 gtaccgccct gtggccatcg aaaattttgc cgtggtccga tcttatgacc ggtaagattg
     4081 gtgaaaatcc gggttacgat ccgctgcaat tcatgctcga cgaagcccac aagcgtggga
     4141 tgaaagtaca cgcctggttt aacccctatc gcgtatcggt taatacgaag cccggtacta
     4201 tcagggaact gaatagcact ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg
     4261 actggatcag aacgtctggc gatcgctttg tcctcgaccc gggcatccct gaggttcagg
     4321 actggatcac atcaatagtc gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt
     4381 ttgacgacta tttctatact gaatcaccgg gttcacggct aaatgataac gaaacgtacc
     4441 gtaaatacgg aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat actcagcagt
     4501 taattgcaaa ggtatcacac accattaaaa gcattaagcc gggagtcgaa tttggcgtta
     4561 gcccggcagg cgtgtggcgt aaccgatcac acgatccgct cggttccgat acccgaggcg
     4621 cggcagccta tgacgaatcc tacgcagaca cccgtcgatg ggtggaacaa ggattgctgg
     4681 attacattgc tccccaaatt tactggccgt tctcacggag tgccgcgcgt tatgacgtgt
     4741 tggcaaaatg gtgggcggat gtcgttaaac caaccaggac ccgcctgtat atcggtatcg
     4801 ccttctataa agtgggtgaa ccttcaaaga tagagccaga ctggatgatt aacggcggcg
     4861 taccggaact gaaaaagcag ctcgatctta acgatgcggt accagaaatt agcggcacca
     4921 tcttgttccg tgaggactat ctgaataaac cgcagactca acaagcggtc agctatctgc
     4981 aaagtcgttg gggcagttaa actaccatgc ttactttgta acaagccggt gctttaccca
     5041 ccggcttgtt acaccttgtg aaatatccca ctagtaatca tgcttacata agtcaaatta
     5101 accacgcaat taaccacact tagttaaaca gctataaatc cctaataaaa atgtatgaat
     5161 atgaaaaacc atacgatatt cgctgtcacc gaaaaataat tacgatgggc tatactcttc
     5221 tgaagaacag tagcgtagat tgaaactgtc ttaagtcgct tatttctttt cagtatatct
     5281 tcatatttca ggagaatatg gtgcagttag tcgatctcgc acgttgcgtt tcgttttgat
     5341 tccgtctgat ttcccagcct tataagggtg tgaattatgg agatgtattt taaaagaatg
     5401 aaagatgagt ggaccggact tgtcgaacag gcagatcctc tcattcgtgc taaagccgca
     5461 gaaattgccc ttgcgcatgc tcattatctg agtatcgagt tttatcgaat tgtccgcatc
     5521 gacccgcatg ccgaagaatt cttgagtaat gaacaagttg agcggcagtt gaaaagtgcg
     5581 atggaacgct ggattattaa cgtgctttct gcccaggttg acgatgtcga aaggctaata
     5641 caaatccagc ataccgtcgc ggaagtgcat gcccgcatag gaattccggt agaaattgtc
     5701 gagatggggt ttcgggtgct gaaaaaaatc ctctatccgg taatcttctc ttcggattat
     5761 tccgccgcag aaaaacttca ggtctaccat ttctcgatta acagtattga tatcgcgatg
     5821 gaagtgatga cccgcgcgtt tacctttagt gacagtagtg cctcaaagga agatgaaaac
     5881 tatcgtatct tctcgttact ggaaaacgcc gaagaagaaa aagaacggca aatagcctca
     5941 atactttcat gggaaataga tattatctat aaagtcctgc tggattctga tttaggcagc
     6001 agtttgcctt taagccaggc tgattttggc ctgtggttta accataaagg tcgacattat
     6061 tttagcggca ttgccgaagt gggccatatc tcccgtctga ttcaggattt cgacggtatt
     6121 ttcaatcaaa ccatgcgtaa caccaggatt ttgaataaca gaagtttgcg ggtgaaattt
     6181 ctattacaga taagaaatac cgtatcgcag attattacct tgctgcgtga attgtttgaa
     6241 gaagtatcgc gccacgaagt cggtatggat gtactgacga aattacttaa ccgccgtttc
     6301 ctgccgacta tcttcaaacg cgaaattgcc catgccaacc ggaccggtac accgctgtca
     6361 gtgctgatta ttgacgttga taaattcaaa gagatcaacg atacgtgggg ccataacact
     6421 ggcgatgaaa ttctgcgtaa agtctcattc ctctcgcaga agagattggt gaaatcgaaa
     6481 atattgggcg ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg cgtagccaga
     6541 atattcatat cccggcgtta tccgtgaact tgtcggcgct gcactttcgc agtaatcagc
     6601 tgcctaatca ggtgtctgat gcaatgcaag cctggggtat tgacggccac cagctgacgg
     6661 tggaaatcac ggaaagcatg atgatggaac acgataccga aatctttaag cgcattcaga
     6721 tcctgcgtga tatgggcgtg ggtttatcgg tagatgattt tggcacgggc ttttccggat
     6781 tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagc tttgtcgatc
     6841 gttgtctgac ggaaaaacgc atcctggcct tacttgaagc cattaccagc attgggcaaa
     6901 gcctcaattt aaccgtcgtg gcggaagggg tcgaaaccaa agagcaattt gagatgctac
     6961 gtaagatcca ctgtcgcgtt attcagggat atttcttttc ccgccccctt cccgccgaag
     7021 aaattccagg ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt cctctcgccc
     7081 gcactcgcgg gttttccttt aaggtgacac tgtcacttaa atactgtgat ttcagctacg
     7141 attccgggag attccttcct taactttccc tcttgcacca aaactgcact acgctgcaca
     7201 aatgttgatc aaaagttaaa aaatataact ttaagccagc attctggcgc ttatcccggc
     7261 atggcatgag atctgcataa gcggaaagcg cagcaatttt tgtcttatac aggaaccgtt
     7321 tatgtcggat accaccgaac tggttgattt agccgtgatc ttccctgatc tggagatcga
     7381 attgaaatat gcctgcgttg ataacatcac aggtaaagct atttatcagc aagcgcgttg
     7441 tctgctacat aaagatgcga ttaccgcgct ggcgaaaagt atcagtatcg cccagctgtc
     7501 aggcttacaa ctggtgattt acgatgcgta tcgcccacaa caagcacagg cgatgttgtg
     7561 gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg
     7621 tggcacggcg ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg atgctaagcg
     7681 aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aatctggtat
     7741 ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgcgac
     7801 tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt gatttaaccg
     7861 cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc
     7921 tgtttagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta gtggtggtcg
     7981 ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg
     8041 cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga
     8101 caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta
     8161 ttgtgcgaat tcccttttat gtgcgcttgg cgcgggggca agcattagtt gtacgccagt
     8221 atacctatgt tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg atcaactggc
     8281 atattttacg taactcccta ccgccgctga tcgtgcaggc atcgctggat atcggtagcg
     8341 cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg
     8401 aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg tggtattgcg
     8461 catttccggg ggcagcgatt ttgcttacct ccgtcgggtt taatctcttt ggtgatggta
     8521 ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga
     8581 cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg cgctcaacaa
     8641 tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat ccggctcagg
     8701 taaatcagtc accgcaatgc tgattatgcg tctgctaccg acgggcagtt attgcgtaca
     8761 tcgggggcag atttcactgc tgggagaaga tgttcttaac gctcgggaaa aacagcttcg
     8821 tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg cccttaatcc
     8881 gacacgtcga ataggtcttc agatgatgga cgtaatccgc catcatcaac caataagtcg
     8941 tcgggaagcc agagctaaag cgattgcact gctggaagag atgcaaatcc cggatgccgt
     9001 ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat
     9061 tgcgccggca ttctcctgcg agccgcaatt gattattgcc ggcgaaccga cgacggcgct
     9121 ggacgtcaca gtacagttgc aggtactgcg tttgcttaaa cataaagcac gcgccagtgg
     9181 aactgcggta ctgttcatca gccatgatat ggccgtggtg tcgcaactgt gcgatagcgt
     9241 ttacgtgatg tatgccggaa gcgtaattga aagcggcggg acggcagacg ttatccatca
     9301 tccccggcat ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg
     9361 ccagccatta cccgccattc cagggacggt accaaacctc acccatttgc ctgacggctg
     9421 cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gcgctgacag cgtgtggtga
     9481 caacaaccag cactgcacct gttggtatcc gcagcaggag gtcataagtg tctgacacgt
     9541 tattaacgtt acgcgacgtc catatcaatt ttccggcccg taaaaactgg cttggcaaat
     9601 ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct
     9661 tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca
     9721 tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg
     9781 tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag
     9841 aatcgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt
     9901 tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg
     9961 gcgggcagcg gcaacgcatc gccattgcca gagcactctc ttcgcagcct gacgtgattg
    10021 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttgc
    10081 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    10141 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    10201 gcgacgcgca acaggtgttg actgcttcgg cacatccata cacccgatta ttgctggatt
    10261 ccctccccgc cattgataaa ccgctggagg aagaatgagc attacgtaaa acggatctgc
    10321 cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgccct ctggcaaccc
    10381 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgac ctccggtgct
    10441 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    10501 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    10561 gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    10621 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt
    10681 atccagcgac acatcggcgg tggtatcaat tgatgttggc gtgaatcccg cttcccccag
    10741 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    10801 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    10861 gccgctctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    10921 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    10981 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    11041 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaag
//
LOCUS       DBBBSB010000126        11021 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.126, whole genome shotgun sequence.
ACCESSION   DBBBSB010000126 DBBBSB010000000
VERSION     DBBBSB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.126"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            111..1010
                     /gene="lysO"
                     /locus_tag="ACIUS0_003379"
     CDS             111..1010
                     /gene="lysO"
                     /locus_tag="ACIUS0_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGB0220995.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1154..2806
                     /gene="hcp"
                     /locus_tag="ACIUS0_003380"
                     /gene_synonym="priS"
     CDS             1154..2806
                     /gene="hcp"
                     /locus_tag="ACIUS0_003380"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGB0220996.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2818..3786
                     /gene="hcr"
                     /locus_tag="ACIUS0_003381"
     CDS             2818..3786
                     /gene="hcr"
                     /locus_tag="ACIUS0_003381"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGB0220997.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3919..5637
                     /gene="poxB"
                     /locus_tag="ACIUS0_003382"
     CDS             3919..5637
                     /gene="poxB"
                     /locus_tag="ACIUS0_003382"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGB0220998.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5674..6675
                     /gene="ltaE"
                     /locus_tag="ACIUS0_003383"
     CDS             5674..6675
                     /gene="ltaE"
                     /locus_tag="ACIUS0_003383"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGB0220999.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6686..8116
                     /locus_tag="ACIUS0_003384"
     CDS             6686..8116
                     /locus_tag="ACIUS0_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGB0221000.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8215..9228
                     /locus_tag="ACIUS0_003385"
     CDS             8215..9228
                     /locus_tag="ACIUS0_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGB0221001.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACIUS0_003386"
     CDS             complement(9225..10055)
                     /gene="amiD"
                     /locus_tag="ACIUS0_003386"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGB0221002.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10052..10375)
                     /locus_tag="ACIUS0_003387"
     CDS             complement(10052..10375)
                     /locus_tag="ACIUS0_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGB0221003.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10586..>11021)
                     /locus_tag="ACIUS0_003388"
     CDS             complement(10586..>11021)
                     /locus_tag="ACIUS0_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGB0221004.1"
                     /translation="KCLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICAR
                     DIYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSI
                     EILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2529 a         2915 c         2894 g         2683 t
ORIGIN      
        1 ccccctctga tgcataacat ttccttttaa ttgcaacttt actcgccctt ccgctttcag
       61 tagtgtgtca tcgcgcattt cgacttcttc tttgcaagga ctggattttc atgttttctg
      121 ggctgttaat cattctggtt cccctgattg tgggttacct cattccgctt cgccaacaag
      181 ctgcgttaaa agctattaat cagctattaa gctggatggt ttaccttatt ctctttttta
      241 tgggtatcag tctggcgttt ctcgataacc tcgccagtaa cctgttggcg attctgcatt
      301 attctgccgt cagtattacc gttattttac tgtgtaatat tgccgccctg atgtggctgg
      361 agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa actcccgtcg cgtattgcga
      421 tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat tggttttgcc attggtctga
      481 gtggactggc tttcttacaa cacgcgaccg aagccagtga atacacgtta attttgctac
      541 ttttcctcgt tggtattcag ttgcgcaata atggcatgac cttaaagcag attgtcctta
      601 atcgccgggg aatgattgtc gccgtggtgg tggttgtcag ttcattaatt ggtggtttaa
      661 ttaacgcctt tattcttgat ctccccatca ataccgcgct ggcaatggcc tccggtttcg
      721 gctggtattc tctttccggt attttattga ccgaatcttt tggtccggta atcgggagcg
      781 cggcgttttt taatgatctg gcccgtgaac tgattgctat tatgttgatc cctgggctga
      841 ttcgccgcag ccgctctact gcactgggct tatgcggtgc cacatcaatg gatttcaccc
      901 tgcccgttct tcaacgtact ggcgggctgg atatggtccc ggcggcaatt gttcacggtt
      961 ttattcttag cctgttagtg ccgatcctca tcgccttttt ctctgcgtaa tacctctctg
     1021 gcggtagttc cctgccgcca aaattgcgct aaatcaatct cccttaaagt tgcatgaaaa
     1081 atccctttta tccccgcgtt aagcgtctta cccttaaaca tgtatattaa atataacttt
     1141 aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact atccgtactc cggcagaaaa
     1201 cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg gaaacttctg accttcagga
     1261 tttactcatc gcggcgctgc aagggctttc tgcctgggcg gtaaaagcgc gtgaatacgg
     1321 catcatcaac cacgatgttg acagcttcgc gccacgtgcc tttttctcaa ccctgaccaa
     1381 cgttaacttc gattctccgc gtattgtcgg ctacgctcgt gaagcgattg ccttgcgcga
     1441 ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg cgcgtcgata acccgatggc
     1501 tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa cgtcaggcag cagaatttac
     1561 ccctaacaaa gataaagcgg cgattggcga aaacattctc ggcctgcgtc tgctgtgcct
     1621 gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg catgttctcg gtcaatacga
     1681 caacgatatt tatgcccagt accataaaat catggcgtgg ctggggacct ggcctgccga
     1741 tatgaacgcg cttcttgagt gttcaatgga aatcggccag atgaacttca aagtgatgag
     1801 cattctggat gcaggcgaaa ccggtaaata cggtcacccg acgccaaccc aggtcaacgt
     1861 caaagcgacg gcgggtaaat gcattctgat ttccggtcac gatctcaaag atctctacaa
     1921 cctgctggaa cagaccgaag gcacgggcgt taatgtctac acccacggcg aaatgctgcc
     1981 tgcgcatggc tacccggagc tgcgtaaatt caagcatctg gtcggtaact acggcagcgg
     2041 ctggcagaat cagcaagtgg agttcgctcg tttccctggc cccatcgtga tgacctcgaa
     2101 ctgcatcatc gacccaaccg taggcgctta tgacgatcgt atctggaccc gcagcatcgt
     2161 tggctggcct ggcgtgcgtc atctggatgg cgaagatttc tctgcggtca tcgcccaggc
     2221 gcaacaaatg gcgggcttcc cgtacagcga aattcctcac ctgatcaccg tgggctttgg
     2281 tcgccagacg ctgcttggtg cagccgatac gctgattgat ctggttagcc gtgaaaaact
     2341 gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc ggcgagcgcc actacttcac
     2401 cgatttcgcc accagcgtgc cggatgactg cctgatcctg accctcgcct gtggtaaata
     2461 tcgctttaac aaacttgagt ttggcgatat cgaaggtctg ccgcgtctgg tagatgcagg
     2521 tcaatgtaac gatgcttact cagcaattat tctggctgtc actctggcag agaaactggg
     2581 ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc tggtttgaac agaaagcgat
     2641 tgtcattctg ctgacgctgc tttctctggg cgtgaaaaat atcgtcaccg gcccgactgc
     2701 acctggtttc ctgacaccag acctgctggc ggtgctgaac gagaaattcg gcctgcgttc
     2761 tatcaccact gttgaagaag acatgaagca actgttgaac gcgtaaggag tttgtcgatg
     2821 acgatgccaa cgaatcaatg cccgtggcgg atacaggttc atcacattac gcaagaaacg
     2881 ccggatgtgt ggacgatttc cctgatttgc cacgatcact acccatatcg cgccgggcaa
     2941 tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc gtgcttacac catttcctcc
     3001 acgccaggcg tgagtgaata tatcaccctg accgtgcggc ggattgatga cggtgtcggc
     3061 tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc tctggctttc ggacgcgatg
     3121 ggggaattta cctgcgacga taaagcagaa gataaattcc tgttgctggc ggcaggctgc
     3181 ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga agaaccgtcc acaggccgat
     3241 gtgcgggtga tctacaacgt gcgtacgccg caggatgtta ttttcgccga tgagtggcgt
     3301 aactatccgg taacgctggt ggcagaaaat aacgttaccg aaggctttat cgctggtcgc
     3361 ctcactcgcg aactgctggc agatgtacct gacttagctt cacgtaccgt gatgacctgc
     3421 ggcccggctc cgtatatgga ttgggtagag caggaagtga aagcgctcgg cgtgacgcgt
     3481 ttctttaaag agaaattctt caccccagta gcagaagcgg cgaccagcgg tctgaaattc
     3541 accaaactgc aaccggcacg agaattttac gccccggttg gcaccacgct actggaggcg
     3601 ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg cgggtgtttg cggctgctgt
     3661 aagacaaaag tggtttccgg tgaatatacg gtgagcagta caatgacgct gaccgacgcc
     3721 gaaatcgctg aaggttacgt actggcctgc tcctgccatc cgcaggggga tttggttctc
     3781 gcataatcgc cttatgcccg atgatattcc tttcatcggg ctatttaacc gttagtgcct
     3841 cctttctctc ccatcccttc cccctccgtc agatgaacta aacttgttac cgttatcaca
     3901 ttcaggagat ggagaaccat gaaacaaacg gttgcagctt atatcgccaa aacactcgaa
     3961 tcggcaggcg tgaaacgcat ctggggagtc acgggcgact ctctgaacgg tcttagcgac
     4021 agtcttaatc gcatgggcac catcgagtgg atgtccaccc gccatgaaga agtggcggcg
     4081 tttgccgctg gcgctgaagc acaactttca ggagaactgg cggtctgtgc cggatcgtgc
     4141 ggcccaggca acctgcactt gatcaacggc ctattcgatt gccaccgcaa taacgttccg
     4201 gtactggcga ttgccgctca tattccctcc agcgaaattg gcagcggcta tttccaggaa
     4261 acccacccac aagagctatt ccgcgaatgt agtcactatt gcgagctggt ttccagcccg
     4321 gagcagatcc cacaagtgct ggcaattgct atgcgcaaag cggtgcttaa ccgtggcgtt
     4381 tccgttgttg tattaccggg cgacgtggcg ttaaaacctg cgccagaagg ggcaaccacg
     4441 cactggtatc atgcgccaca gccagtcgtg atgccggaag aagaagagtt acgcaaactg
     4501 gcgcaactgc tgcgttattc cagcaatatc gccctgatgt gtggcagcgg ctgcgcgggg
     4561 gcgcataaag agttagttga gtttgccggg aaaattaaag cacctattgt tcatgcccta
     4621 cgcggtaaag agcatgtcga atacgataat ccgtatgatg ttggaatgac cgggttaatc
     4681 ggattctcgt caggtttcca taccatgatg aacgccgaca cgttagtgct actcggtact
     4741 cagttcccct accgtgcgtt ctacccgacc gatgccaaaa ttattcagat tgatattaat
     4801 ccagccagca tcggcgcgca tagcaaggta gatatggcgc tggtcggcga tatcaaatca
     4861 accctgcgcg cattgctgcc actggtggaa gaaaaaaccg atcgcaagtt tctggataaa
     4921 gcgctggaag attaccgcga tgcccgcaaa gggctggacg atttagctaa accgagcgag
     4981 aaagccattc acccgcaata tctggcgcag caaattagtc attttgccgc cgatgacgcc
     5041 atctttacct gtgacgtcgg cacgccaacg gtatgggcgg cacgttatct gaaaatgaac
     5101 ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga tggctaacgc catgccgcag
     5161 gcgttgggtg cgcaggcaac cgagccggaa cgtcaggtgg tcgccatgtg cggcgatggc
     5221 ggttttagca tgttgatggg cgatttcctc tcagtagtgc agatgaaact gccggtgaaa
     5281 attatcgtct ttaataacag cgtgctgggc tttgtggcga tggagatgaa ggccggaggt
     5341 tacctgacag acggtactga gctgcacgac accaactttg cccgcattgc cgaagcgtgc
     5401 ggcattacgg gtatccgtgt agaaaaagcc tctgaagttg atgaagccct gcaacgcgcc
     5461 ttctctatcg acggtccggt gttggtggat gtggtggtcg ccaaagaaga attagccatt
     5521 ccaccgcaga tcaaactcga acaggccaaa ggttttagcc tgtatatgtt gcgcgcaatt
     5581 atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa cgaactggct aaggtaaaaa
     5641 gggtggcatt tcccgtcata ataaggacat gccatgattg atttacgcag tgataccgtt
     5701 acccgaccga gccgcgccat gctcgaagcg atgatggccg ccccggttgg ggacgacgtt
     5761 tacggagacg accctaccgt taatgctctg caggactatg cagcagagct ttccggtaaa
     5821 gaagccgcca tttttctgcc taccggcact caggccaacc tggtcgctct gctcagtcac
     5881 tgcgaacgtg gcgaagagta tattgtcggt caggccgcgc ataactatct gtttgaagcc
     5941 ggtggcgcag cggtgctggg cagtattcag ccgcaaccca tcgacgcggc tgccgacggc
     6001 acgctaccgc tggataaagt ggcgatgaaa atcaaacccg acgatatcca tttcgcccgc
     6061 accaaattac tcagtctgga aaacacccac aacggcaaag tgctgccgcg tgaatacctg
     6121 aaagaagcat gggaatttac ccgcgagcgc aatctggcgc tgcatgttga cggtgcgcgc
     6181 atctttaatg ccgtggtggc ttacggctgc gaactgaaag agatcacgca atattgtgat
     6241 tcgttcacca tttgcctgtc gaaaggtctt gggacgccag tcggttcatt actcgtcggt
     6301 aatcgtgatt acattaaacg tgccatccgc tggcggaaaa tggccggtgg cgggatgcgt
     6361 cagtccggga ttctggctgc cgccgggatg tatgccctgg aaaataacgt cgcacggttg
     6421 caggaagatc acgacaacgc cgcctggatg gcggagcaac tgcgtgaagc aggcgcggat
     6481 gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg gggaagaaaa tgctgccgcg
     6541 ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg cctcgccgat tgtccgcctg
     6601 gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag tcgccgccca ctggcgcgca
     6661 ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt ttagttctcg gtgccagtgg
     6721 ctacattggt cagcatctgg tgcgcacact cagccagcaa gggcatcaga tcctagcggc
     6781 ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca aacgtcagtt gccataaagt
     6841 cgatctcagc tggccggata accttccggc tctgttgcag gatatcgata ccgtctattt
     6901 tctggtgcac agcatgggcg aaggcggcga ttttatcgct caggagcgcc agctggctct
     6961 caacgtccgc gatgcgctac gtgaagtacc agttaagcaa ttaatctttc tcagttcgtt
     7021 gcaggccccg ccacatgagc agtcggatca tctgcgagct cgtcaggcta cggcggacat
     7081 tcttcgtgaa gcgaatgtac cagtgaccga acttcgggcc ggaattatcg ttggcgcagg
     7141 ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac ctgccagtgt taacgccgcc
     7201 acgctgggta cgttcacgca ccacgcccat cgcgctggaa aacttgctgc actatctggt
     7261 ggcgctgtta gaccatccag ccaacgaaca ccgcatcttc gaagccgccg gaccagaggt
     7321 gctcagttat cagcaacagt ttgaacattt tatggcggtg agcggtaagc gccgctggtt
     7381 gatccccatc cccctcccca cccgctggat ttcggtgtgg tttctcaatg tgattacttc
     7441 cgtaccgccc accaccgcca gggcgttgat tcaggggctg aaacacgatc tgctggcaga
     7501 tgataccgcg ctacgtgcac tcatcccaca acggctgatc gctttcgatg acgcggtacg
     7561 tagcacgttg aaagaggaag aaaaactggt caactccagc gactggggct acgacgctca
     7621 ggcctttgcc cgctggcgac cggagtacgg ttatttcgcc aaacaggcgg ggtttaccgt
     7681 taaaacgtcc gccagccttg cggctttatg gctggtggtg aaccaaatcg gcggtaaaga
     7741 gcgttatttc tttggcaata ttttgtggca gacacgggcg ttgatggacc gcgcgatcgg
     7801 tcataatctg gcgaaaggcc gcccggagcg cgaatattta cagaccggcg atgcggtgga
     7861 tagctggaaa gtgattgtcg ttgaaccgaa aaagcaactt acgttgttat ttggcatgaa
     7921 agcgccgggg ctgggacgac tgtgttttac cctggaagat aaaggtgact atcgtactat
     7981 cgatgtccgc gctttctggc atccgcacgg tatgccgggg ctgttttact ggttattgat
     8041 gatccccgcg catctgttta tttttcgcgg aatggcaaaa caaatcgcca gactggcaga
     8101 acaaagcaca gattaatcga caaaacctgc gttattcttt catgtttccc attgcatcat
     8161 gggggaaatc acggaagaat gcgcacgtca ttttctcaac aacagtggat tgatatgaag
     8221 gtactggtta ccggcgccac cagcggctta ggtcgaaacg cggtagagtt tttatgccag
     8281 aaaggcatca gcgtgcgtgc gagcggtcgc aacgaagcaa tgggtaaatt gctggaaaaa
     8341 atgggcgcag agtttgttcc gacggatctg accgagctgg tttcatcaca agctaaagtg
     8401 atgctcgcgg gcattgatac gctgtggcac tgctccagct ttacctcacc ctgggggaca
     8461 caacaggctt tcgatctggc taacgttcgc gccactcgcc gcctgggtga atgggctgtc
     8521 gcctggggtg tgcgtaactt tattcatatc tcttccccct ccctgtactt cgattatcac
     8581 caccatcgca atattaaaga agattttcgc cctcaccgct tcgccaacga gtttgcccgc
     8641 agcaaagcag ccagcgaaga agtgatcaat atgctttcgc aggcgaatcc acaaacgcgc
     8701 tttactattc tgcgcccaca aagtctgttc ggaccgcacg ataaagtctt tattccccgt
     8761 ctggcgcata tgatgcacca ctacggcagt attctgttac cgcatggcgg cagtgcgctg
     8821 gtcgatatga cctactatga aaatgccgtg cacgcaatgt ggctggcaag ccaggaagcc
     8881 tgcgataagc taccttccgg gcgtgtgtac aacatcacca acggcgagca tcgcacactg
     8941 cgcagcatcg tgcagaagct gatcgacgag ttgaatattg actgtcgtat tcgttccgtc
     9001 ccttacccga tgctggatat gatcgcccgc agcatggagc gtttaggccg caagtcagca
     9061 aaagagccgc cgctgaccca ctacggcgcc tccaagttga attttgactt tacgctggat
     9121 attacgcgtg cgcaggaaga gttaggttat cagccggtca tcaccctgga tgaaggcatc
     9181 gagaaaactg ccgcctggct acgcgaccac ggaaaactgc cgcgctaatc ctgcccgtat
     9241 ttctccagca atgcttcggc aatcgcctga gtttctgcat ccgcttcgcc gttatataac
     9301 gtcgggcgga aatgcatctg gaatgccata atcacgcgcc gctgctcgcg cggtgtcata
     9361 tcaggtttta cgtcataacc gtagcgcgcc aaaagctcca gcaatgacgc ggtatctacc
     9421 ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttcaccc gctgcgcatc cggccaggca
     9481 ccaatcccct gctgcgccag ttgctgccag ggaaataatg gccccggatc gtctttgcgc
     9541 tgcggtgcga tatccgcatg tgccactacg ttttccggct tgatgtgata acgggcaata
     9601 atatctttcg ccagcggaat aagcgcctga atctgtgcct gttcaaacgg ggcaaaatat
     9661 ttcactccgg atgatttttg ccagccacgg ttttccagct caatgccaat agaggtgtcg
     9721 ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat gccaggccag ttcttgttcc
     9781 ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg gtggcaccgc agggatcaga
     9841 taatgcgagc tgacctgctt atcagtcagt gtcgccagcg agctatcaaa atcatctgcg
     9901 gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg cctgcgcctg gtgtcgggta
     9961 tcaagctgat atccctcttt ctcgacaatg cctttttcgc ctgcacaccc tgccagtaac
    10021 agagcggcag cgaccagcca gaagactctt ctcatcgacg cgttttcacc gccgtaccgc
    10081 tgacgctaac catcagcata ctgccgtttt gcccgaccgt ttcgtagtcg atatcaatac
    10141 caactacggc atcggccccc agcgcccgcg cctgggagcc taattcctca aaggcgatct
    10201 cccgtgcttt acgcagttct ttttcatacg caccggaacg tccgccaacg atatcgcgga
    10261 taccggcaaa gaaatcacgg aaaatattgg cacctaaaat cgcttcgccg gtcaccacac
    10321 cgcaatattc aacgatggtc aggccttcca gagttggggt tgtagaaaat tgcatgatcg
    10381 atctccttgt ttgttaagcc ttcgatctct aaagcattat cggactgata tgccatgttg
    10441 ggaagaaggt tttgatcagt ttaggaaatc atctcattgt gtcaaaaaca cccacttaag
    10501 tctttacgat tatccgttgc ataacccgat attggtttca ttatagggta ccgcttacct
    10561 gtcttattat gttgcaccaa caacattaat gagcggaaag aggcaactta tattgtaaat
    10621 gactcctaaa gtcttcccca tcttgtgact gaggtctata ccgctggatt gctttttggt
    10681 cagccgccgc aaaacaagaa agtaataaga tctcaatgct tttgtcatta atataagcga
    10741 agatcattct tgcaaactcg tatataaacg cccgatcctg agcgcttaat agtttaatac
    10801 aatatttatt tttaagagag aaaataattt caaggaaagc atccgcagca accttcacgt
    10861 tattcccggc gttcatatta tatatatccc ttgcacagat agagtacaga gatagtaaac
    10921 agccatacaa ataatcactt attaattctt ttgtccattt tacaggcaac cagggtttta
    10981 tcttgacacc atgcatacta cattcatgaa aaagacattt g
//
LOCUS       DBBBSB010000127        10937 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.127, whole genome shotgun sequence.
ACCESSION   DBBBSB010000127 DBBBSB010000000
VERSION     DBBBSB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10937
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.127"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            88..849
                     /gene="adiY"
                     /locus_tag="ACIUS0_003389"
     CDS             88..849
                     /gene="adiY"
                     /locus_tag="ACIUS0_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGB0221005.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            986..2323
                     /gene="adiC"
                     /locus_tag="ACIUS0_003390"
     CDS             986..2323
                     /gene="adiC"
                     /locus_tag="ACIUS0_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGB0221006.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2427..4070
                     /gene="eptA"
                     /locus_tag="ACIUS0_003391"
     CDS             2427..4070
                     /gene="eptA"
                     /locus_tag="ACIUS0_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGB0221007.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            4067..4735
                     /gene="basR"
                     /locus_tag="ACIUS0_003392"
     CDS             4067..4735
                     /gene="basR"
                     /locus_tag="ACIUS0_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGB0221008.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4736..5836
                     /gene="pmrB"
                     /locus_tag="ACIUS0_003393"
     CDS             4736..5836
                     /gene="pmrB"
                     /locus_tag="ACIUS0_003393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGB0221009.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACIUS0_003394"
     CDS             complement(5812..5901)
                     /gene="pmrR"
                     /locus_tag="ACIUS0_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGB0221010.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACIUS0_003395"
     CDS             complement(6013..7515)
                     /gene="proP"
                     /locus_tag="ACIUS0_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGB0221011.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7779..8657)
                     /locus_tag="ACIUS0_003396"
     CDS             complement(7779..8657)
                     /locus_tag="ACIUS0_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGB0221012.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="ACIUS0_003397"
     CDS             complement(8654..10882)
                     /gene="crfC"
                     /locus_tag="ACIUS0_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGB0221013.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2735 a         2863 c         2684 g         2655 t
ORIGIN      
        1 tattcatcat gcaattatat aaccatttcc cggtatcgct ttgctttagc gagaactggt
       61 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
      121 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      181 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaac
      241 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      301 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact caccctgcca
      361 tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact
      421 gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg ttttctcgac
      481 agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      541 tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt agccagttgt
      601 ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac cagttatagc
      661 caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat ggacggtaaa
      721 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      781 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      841 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      901 gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga
      961 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
     1021 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1081 tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga cgattatcgg
     1141 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg
     1201 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1261 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1321 aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt
     1381 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gcgtgcaggc
     1441 cgttgccact gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1501 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc
     1561 gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1621 tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca ttggtggggt
     1681 attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga tgatccctaa
     1741 tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1801 caccgctggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1861 ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1921 gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta tcgtcggtat
     1981 tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag agttcggtct
     2041 ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2101 actgctgctc ggccacggtc actttggtaa agcacgcccg gcatatctgg ctgttactac
     2161 cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag aggttatgtg
     2221 gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca accggctaca
     2281 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca
     2341 aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2401 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga
     2461 atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct
     2521 tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta ctggttttct
     2581 tgtcgatgcc ggttgtcgct ttcagcgtga ttaatattgt cctgacacta agctctttct
     2641 tatggcttaa tcgaccactg gcctgcctgt ttattctgat tggcgcggct gcacaatatt
     2701 tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca
     2761 ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg ggattcagcg
     2821 gcgtgcttgc tgagctgatt gcctgctggc taaaaatcaa acctgccacc tcgcgtctgc
     2881 gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt ttgctggtcg
     2941 ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag ctggtgaaat
     3001 ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg
     3061 caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta atgcagaacg
     3121 aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct
     3181 ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac gtggtctatt
     3241 tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg
     3301 atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3361 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg
     3421 cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat cagtgcatca
     3481 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3541 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3601 gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct
     3661 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3721 ttgataaagc gattaatctg cttaaagaac atcaggataa atttaccacc agcctggttt
     3781 atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt
     3841 atgccatcgc cccggatagc caaaaacagg tgccaatgct gctgtggctg tcggaggatt
     3901 atcaaaagcg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact
     3961 attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     4021 accaggccgc ggatgatatt ctgcaaactt gcaggagagt gagtaaatga aaattctgat
     4081 tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta
     4141 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg acggtcatta
     4201 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4261 tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg atacgctgac
     4321 cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct
     4381 ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4441 tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg
     4501 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4561 aggcagtccg gtacatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4621 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4681 tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa actaattgaa
     4741 tctgatgcgt tttctgcgcc gacctatatc gctgcgccaa cggctgatat tgaccatcgg
     4801 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4861 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4921 gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct
     4981 gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca
     5041 aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc acagcgccac
     5101 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5161 gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5221 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5281 agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc
     5341 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5401 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc
     5461 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5521 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5581 gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta ttgatgagag
     5641 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5701 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5761 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5821 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5881 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5941 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     6001 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc
     6061 ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat tatcgtaatg
     6121 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6181 cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca ccgccactac
     6241 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6301 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6361 acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgccca taaagcagtt
     6421 aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat
     6481 cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta gcacaaacgg
     6541 acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6601 cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa
     6661 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6721 aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg
     6781 gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc
     6841 cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat
     6901 aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat
     6961 taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     7021 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt
     7081 atattcacca ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7141 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7201 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7261 gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7321 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7381 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7441 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg gttttacttt
     7501 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7561 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7621 tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7681 attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac
     7741 atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt tacagagaag
     7801 atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg catccccagc
     7861 gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc
     7921 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag
     7981 gcaaaccact gatggtaata gtgacgcgta gtcggtgccg cattgatact ctctgccaga
     8041 tgttgtgtcg ctttctcgcg caaatcaccc agcaacaaca ccaccgcttg cgggcttccc
     8101 agacggcagg catcgccaaa tgcaccccag cgcaactcct ccagaacgat aaaacagcgc
     8161 ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat ttgctcgctc
     8221 tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8281 cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag
     8341 tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc
     8401 agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg caggctaagc
     8461 ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga aagaagttcc
     8521 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgag gatggcgggc atcatcgccc
     8581 tgcgccagat ccactaaaaa gcgcggatga accgactcca gcacgcgacc ggggccgtcc
     8641 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8701 gctgagcgtc ggtgtaaatc cagctgtgag tcatcacagt ctgctgcaat tgctgctgca
     8761 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8821 gtaattctgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8881 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaatagaga tcgacttctg
     8941 cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat ttctcatttc
     9001 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9061 gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcgag
     9121 tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga cgaaaccctg
     9181 actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg gttaacaccc
     9241 ggcaaagggt ggattccatc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct
     9301 gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc tccgaatcat
     9361 cctgaaacaa ggcggctaac tgcgcattca ccgcatcttg ctgttgacgc agaaagtggt
     9421 tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat
     9481 tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc aattgttcac
     9541 aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat
     9601 tcaacagttt atgcgcagca gatcgcagag catacaatga ggcgttagcg taagcggcat
     9661 gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat
     9721 gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat
     9781 gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg ttgttggcta
     9841 actcatagcg cgcccgattc gccaggtagc cccacatcga cgacaccggg aatatctgct
     9901 gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt
     9961 cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt
    10021 gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat
    10081 aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca
    10141 gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat
    10201 aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg
    10261 cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc actaaatcat
    10321 tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt tcgaaagcga
    10381 cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg
    10441 tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt aaacaatcga
    10501 ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta tggcgaataa
    10561 gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta ccaacaatgg
    10621 cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc accatttcca
    10681 gtcgggaaat tttacacaac tcattattca gcatcgcgtg acgttcggcg atattaggct
    10741 gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga ggcatttttt
    10801 ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc tgacttaact
    10861 catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg ttacttttat
    10921 ttagccagat tgttttt
//
LOCUS       DBBBSB010000128        10857 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.128, whole genome shotgun sequence.
ACCESSION   DBBBSB010000128 DBBBSB010000000
VERSION     DBBBSB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.128"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            3..914
                     /locus_tag="ACIUS0_003398"
     CDS             3..914
                     /locus_tag="ACIUS0_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGB0221014.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            1049..2269
                     /gene="mlc"
                     /locus_tag="ACIUS0_003399"
                     /gene_synonym="dgsA"
     CDS             1049..2269
                     /gene="mlc"
                     /locus_tag="ACIUS0_003399"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGB0221015.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2394..3089
                     /gene="bioD"
                     /locus_tag="ACIUS0_003400"
     CDS             2394..3089
                     /gene="bioD"
                     /locus_tag="ACIUS0_003400"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGB0221016.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3042..4334)
                     /gene="clcB"
                     /locus_tag="ACIUS0_003401"
     CDS             complement(3042..4334)
                     /gene="clcB"
                     /locus_tag="ACIUS0_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGB0221017.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4493..5107)
                     /gene="dmsD"
                     /locus_tag="ACIUS0_003402"
     CDS             complement(4493..5107)
                     /gene="dmsD"
                     /locus_tag="ACIUS0_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGB0221018.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5150..6004)
                     /gene="ynfH"
                     /locus_tag="ACIUS0_003403"
     CDS             complement(5150..6004)
                     /gene="ynfH"
                     /locus_tag="ACIUS0_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGB0221019.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(6006..6623)
                     /gene="ynfG"
                     /locus_tag="ACIUS0_003404"
     CDS             complement(6006..6623)
                     /gene="ynfG"
                     /locus_tag="ACIUS0_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGB0221020.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6634..8672)
                     /locus_tag="ACIUS0_003405"
                     /pseudo
     CDS             complement(6634..8672)
                     /locus_tag="ACIUS0_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(8733..9767)
                     /locus_tag="ACIUS0_003406"
                     /pseudo
     CDS             complement(8733..9767)
                     /locus_tag="ACIUS0_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            9825..>10857
                     /locus_tag="ACIUS0_003407"
     CDS             9825..>10857
                     /locus_tag="ACIUS0_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGB0221021.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT         2680 a         2769 c         2815 g         2593 t
ORIGIN      
        1 atatgaatat tgaacttcgt catctgcgtt actttgttgc tgttgcggaa gagctgcatt
       61 ttgggcgcgc ggctgcccgc ctgaatattt cgcaaccgcc gctaagtcag cagattcagg
      121 cactggagca acaaatcggt gcccgactgc tggcacgaac taatcgcagt gtattgctga
      181 cggcagcagg aaaacagttt cttgcagata gtcggcaaat cctgtctatg gtggatgacg
      241 ctgccgctcg cgctgaaaga ctgcatcagg gtgaagcggg ggagttgcgt attggtttta
      301 cttcgtccgc tccttttatt cgggcggtgt ccgatacgtt atcgctgttt cgccgtgatt
      361 atcctgatgt ccatttacaa acccgcgaaa tgaacactcg cgagcaaatc gctccgctca
      421 ttgaaggaac gctggatatg ggattgctgc gtaacacagc gttaccggag acgcttgaac
      481 acgcagtcat cgtccatgaa ccgcttatgg cgatgatccc gtacgatcat cccctggcaa
      541 ataatccgag tgtaacactg gctgaactgg ctgaactggc tgaactggcg aaagaaccct
      601 ttgtcttttt tgatccgcac gtcgggactg ggctgtatga cgatattctc ggactgatgc
      661 gacgttacca tttgacgccc gtcatcactc aggaggtggg cgaggcaatg accatcatcg
      721 gtctggtttc cgccggtctg ggtgtttcaa ttttgcctgc gtcatttaaa cgtgttcagc
      781 tcaacgaaat gcgctgggtg ccgattgctg aagaggatgc ggtttctgaa atgtggttgg
      841 tctggccgaa acatcatgaa caaagtccag ctgcgcgtaa ctttcgtatt catctgctga
      901 atgctctcag gtgagggaaa tttcagcgaa aaagcccgaa aaatgtgctg ttaatcacat
      961 gcctaagtaa aaatttgacg acacgtattg aagtgcttca ccatagccta cagattattt
     1021 cggagcgcga aaatataggg agtatgcggt ggttgctgaa aaccagcctg ggcacattga
     1081 tcaaataaag cagaccaacg cgggcgcggt ttatcgcctg attgatcagc ttggtccagt
     1141 ctcgcgtatc gatctttccc gtctggcgca gctggctcct gccagtatca ctaaaattgt
     1201 ccgtgagatg ctcgaagcac acctggtgcg agagctggaa atcaaagaag cggggaaccg
     1261 tggccgtccg gcggtggggc tggtggttga aactgaagcc tggcactatc tttctctgcg
     1321 cattagtcgc ggggagattt tccttgctct gcgcgatctg agcagcaaac tggtggtgga
     1381 agagtcgcag gaactggcgt taaaagatga cttgccattg ctggatcgta tcatttccca
     1441 tatcgatcag ttttttatcc gccaccagaa aaaacttgag cgtctaactt cgattgccat
     1501 aaccctgccg ggaattattg atacggaaaa tggtattgta catcgcatgc cgttctacga
     1561 ggatgtaaaa gagatgccgc tcggcgaggc gctggagcag cataccggcg taccggttta
     1621 tattcagcat gatatcagcg catggacgat ggcagaggcc ttgtttggtg cctcacgcgg
     1681 ggcgcgcgat gtgattcagg tggttatcga tcacaacgtg ggggtgggcg tcattaccga
     1741 tggtcatctg ctacacgcag gcagcagtag tctcgtggaa ataggccaca cacaggtcga
     1801 cccatatggt aaacgctgtt attgcgggaa ccacggctgc ctcgaaacca tcgccagtgt
     1861 ggacagtatt cttgagctgg cacagctgcg tctcaatcaa tccatgagct cgatgttaca
     1921 tgggcaaccg ttaaccgtgg actcattgtg tcaggcggca ttgcgcggcg atctactggc
     1981 aaaagacatc attatcgggg tgggcgcgca tgtagggcgc attcttgcca tcatggtgaa
     2041 tttatttaac ccacaaaaaa tactgattgg ctcaccgtta agtaaagcgg cagatatcct
     2101 cttcccggtc atctcagaca gcatccgtca gcaggccctt cctgcgtata gtcagcacat
     2161 tagcgttgag agtactcagt tttctaacca gggcacgatg gcaggcgctg cactggtaaa
     2221 agacgcgatg tataacggtt ctttgttgat tcgtctgttg cagggttaac attttttaac
     2281 tgttctacca aaatttgcgc tatctcaatt tgggccagga aagcataact tagactttca
     2341 aggttaatta ttttcctagt ttatatttgt gaagcataac ggtggagtta gtgatgctga
     2401 agcgtttctt tattaccggt acagacactt ctgtagggaa aacggtggtt tcccgcgcat
     2461 tgctacaagc gttagcctcc cagggaaaaa gggttgcggg atataaaccc gtagcgaagg
     2521 ggagcaaaga gacacccgaa gggctgcgta ataaagatgc cttggtgttg cagagtgttt
     2581 caaccatcga actgccttat gaagcagtta atcctatcgc gttaagcgaa gaagaaagta
     2641 gcgtggcgca cagttgccca atcaattaca ccctcatttc aaacggcctg gcaaacctga
     2701 ccgataaagt cgatcatgtc gtggtagaag ggactggcgg ctggcgcagt ctgatgaatg
     2761 atttgcgtcc actctctgaa tgggtagtgc aggaacaact gccggtgttg atggttgtcg
     2821 gtattcagga aggttgcatt aaccatgcac tgctaacagc tcaggcgatc gccaacgacg
     2881 ggctgccgct cattggctgg gtggctaacc gaatcaaccc aggactggcg cattatgcgg
     2941 aaatcattga tgtgctgggt aaaaaacttc cggcaccgct cattggtgaa ctgccttatc
     3001 tgccgcgcgc cgaacagcgt gaactggggc aatacatccg cttagctatg ctgcgcagtg
     3061 tgctggcggt agatagagtc acggtgtaac gtccgcgaaa ttaccgacgc aattacgcag
     3121 gcaatcaata aaccggggag tagctgatac tccccggtca tttcacatat catcaacgtc
     3181 gacataatcg gcgcgtgcgt ggttgccgcc aacagtgtcg ccatcccggt caatccgagt
     3241 aaaagcgtaa tttcttcgcc atcagggaac cataatccca ggctacgacc atacaacatg
     3301 ccaatggcaa gaccgataaa tagcgtcggt gtaaagacac caccgggtgc gccggaaccg
     3361 ctactcgcca gcacggcaca cagtttacaa aggaagatcc cggcaatgat cattaacagt
     3421 ggtggggcgg ttaaaaagga ttgtacggtg ctatagccgt tgccccacac tgcaggtgtg
     3481 aacagggaaa gcagacccac gatcaacccg cctagtgcca gttgccaggg cggcgcaagt
     3541 ttgagactca caaatccacg atgacaggcg ttcattaacg ttaacaacag tggtccgcac
     3601 agacctgcca gcacacctgt actgataatc agcgcatagt cacgagcctg aaccgtcact
     3661 gagagttgta cgttgtagag taacgcgtcg ctatgattaa tcagattgct aaccagccat
     3721 gccacgatgg cggaaataat caccgggccg agagaggcca acatcatagt gccaaacagc
     3781 acttcggcta taaataaact gccagcaagc ggggcacgat aggccgcagc cattcccgcc
     3841 gcggccccac aggcgatcca taatttccat tcctggcgtg gcgtaaaacg ttgggcaaaa
     3901 caggaggcgg caagggcagc taaaagaatc atcgcacctt cgcgaccaat tgcactgccg
     3961 ctggttacta ccagcagcga ggcaagcgat ttaaccaggc ttgctgcgta atcgaactgt
     4021 ccatcggttt gcaacgcttc catgtaatcg gtcggcgcat gagggcgttg ttgggtaaat
     4081 ttctgccagc ccatcagcaa caaacccgcc gccagtccgc cgagcgccgg agttagcagc
     4141 cgtcgccagg aggaaaggtt tgtcgctgca ttgaccagac tgccggagtc attattgagg
     4201 aacaaccact ccagtagcag catcgcatga cgaaacccgg caacggcaaa gacagcgaga
     4261 ataccgacga ctgttgcgat aagcagacgg cggaacatag tgcgcagatc cggatagatg
     4321 tgtagatgat gcatgagcag aaatgggcaa tgaaacacgg gcgactattt tgcggcaaaa
     4381 gaggccaacc gcaaaagcag tgcgtgcttt aagagataaa aaaacgtgac attgtaacgt
     4441 attattgccg aatcgtgcgt aatcgtcacc atccggcaat attacggtga tcctatcgaa
     4501 agagtggttt aaccgcgaca ggaatcaaca gttgtgattg ccattgcgcc agcgttaagc
     4561 gagccagttc acccagtgca cggtagaaag ggtgttctgc tttttcgata aaaacatcaa
     4621 gaaaacgtgt tgaccacgga aaaaggtgcc atgccagcaa ttcttcgcat tctgtctggc
     4681 gaccattctc cgccaaccac gcagccatca acagcagtga accaaaatga tcttccggtt
     4741 cgttttgctt catttcaaac tgaatgcctt tctcgcgcat ccactgacga agtgccaatg
     4801 ttgaatcgcc aaacagcaca gattcgcgat ccagccagac cgacccccac ggcggagacg
     4861 gcagtgccca cgggccgaca aacaaacgct gccaggcctg ggcgtgagtc tcttcacact
     4921 gtgtctgaaa agccgtcacc agcggcgcta atgacgcttc tggtagaggc cactgagttt
     4981 cccagccgtc actggtgagt accgcaacca gaggtgcggc ttccgcactc tctggagcgt
     5041 aataaaacag cgcgcccaac acgcgcgccg cgacagaaaa attatcttgc tgtgaaaaat
     5101 gggtcattcc taacatcctg aaatgtgcgg gttgccccgc accaatttgt taacctgcaa
     5161 tagccattcc tactgtcatg tgcaggccat aaaagagcac acgcccgatc atctcaccaa
     5221 caagtatgag gattagcccc agaacaagtc cggcaacgtg aggttcacgg cgacgaatga
     5281 gcggacaaag ccagcatcca agacctgcgc ataacaatac cacacgccag acctgtaagg
     5341 aggcgtaatc tggtaccagt gcgctggctt gttgcacgga tgagtgaata gacgccagag
     5401 acaaaccttg cagcacaatc acctcggcac aagcgatcag cgccagaacg ctaatgatgg
     5461 caaatggtgt ggtattaaat gtaacgcggg ctgcccgcag aattgcggca gcaagtattg
     5521 gaccgctcaa caataccgtc aggaagaaag ccagcgtagt gtaaccgtta tgccaggttg
     5581 gcacggtgtc gatttgatac acgcaggtca tcatccagac gaaaatgacg ccgagcgcca
     5641 tgctgacaag taaccagagt ttccccaacg cttgcggcat tttaccgatg accgccacca
     5701 gccaccatag tccgccaacg gcaaaaaata tggaaccggc agcaatctcg ttactcagac
     5761 cagaagctcc gattcgattc agcgagttaa acgctcgcag tggcgatccc aggtgcatga
     5821 cagaggcaat aaacccgatg cccatcaaca gccagaggaa aaacatgcca cgaacaatgt
     5881 gctgacggtc tgcgtcattt ttggcggcga accagccaat tccgctaaca atcagcgcgc
     5941 ctactacgca ttgacccaat acggtaaaga taaccagtgg ccattcatgc catccatttc
     6001 ccattttaca cttcctccgg attagccaga taaccagtgg tatcgccggt cgggcggctg
     6061 ttggcgttag gtttgataac gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg
     6121 acggcagcca gagtgccgtg cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca
     6181 cgcagcgggc aggattcgac gcatatgggt tgtttcccct cggcgacgcg cgaataacaa
     6241 ccatcgcact tcgtcatgtg ccctttttca gcattgtact gtggtgcgcc gtacggacaa
     6301 gccatgtggc agtagcggca gccgatacaa acatcttcat caaccaccac aaagccatct
     6361 tcacgcttat gcattgcgcc gctggggcag acttttgtac atgcggggtc atcgcaatgg
     6421 ttacaggaga tggagagata ataagcgaac acgttctggt gccagatgcc attatcttcc
     6481 tgccagtcgc cgccagcata ttcataaata cggcggaaac tgacttccgg gccaagatct
     6541 ttaaaatctt tgcacgccag ttcgcaggtt ttacagccag tacagcggct ggaatcgata
     6601 aaaaatccat attgtgtggt catgggctac tccttaaacc ttttcgatct ggacaagatt
     6661 gctgtgcgac gggtttccct ttgccagcgg tgaagggcgg tgagaggtca gaatattgat
     6721 actgccgccg tgatcgaccc ggtcaccaaa catatccgct ttaagccacg caccttgccc
     6781 gatggcggta acgccaggca gaatacgcgg agtcactttt gcggcaatca gcatttctcc
     6841 attattgtta aatacccgca cggtatcgcc atgacggata ccgcgtgcct gagcatcaat
     6901 ggggttgatc cacacctctt gtgggcaggc ctgctgtaac acatcaatat tgccgtagct
     6961 ggagtgggta cgcgctttgg tttggcgact tcactcagca tccagtagat gggtttgcgt
     7021 tcaaattttg ctgaggttgc gggttgggcg aggatcacat agcccatatt ccctgcagat
     7081 tcatgagaga taaggtcttc ttgctctgtt ggcatcaggt caggcaacag gatatcgcaa
     7141 tacttagccg aggccgtcat gaagtggtca atgccaacaa tcatctcgca cttgctgtca
     7201 tcctgaagca cctcatgggt gtgattgatg tcgccatgtt gattgatcaa tgtgttactg
     7261 gcgtagcacc ataaaaactt gatggggaca tccagttttt cttttccacg aacaccatca
     7321 cgggtcgcgg tcattttcgt accatggtcg atggcatctg tccatgtaaa aacggaaatc
     7381 tgcgttttaa caggattctc gagcatcggg aaccattcta cccccagatc ccagctacct
     7441 tcgcgtacac ctgagttgcc gccgtttatg ccgacgttac cggtaagaac ggaaagcatg
     7501 gcaatagcgc gggacgtttg ttcgccgttg gagtgtcgtt gtggccccca accctgacaa
     7561 atataagcag gttttgctga accgatctct cgtgccaact ggataatttt ttctgccggg
     7621 atgctggtga tttttgctgc ccattccggc gttttagcta tgccgtcagg cccttcgccc
     7681 agaatatagg ctttataatg cgcgttacgt ggtgcgttgg cgggcagcgt tttttcatcg
     7741 taaccaacac aatatttgtc gagaaatggc tgatcgacca tgttttcagt aatcagtacc
     7801 caggcaatcg cacaggccag tgcgccatcg gtgccagggc gaatgggcag ccattcatct
     7861 tcacgcccgg cagctgtgtc gttatagcgt ggatcgatga cgatcatgcg tgcgtttgaa
     7921 cgttcgcggg cttgctcgac gtagtaagtg acaccaccgc cgctcatccg cgtttctgcc
     7981 gggttatttc cgaacataac gaccagtttc gtattggcga tatcatccgg gctgttgcca
     8041 tcattggcac cgaacatata actcattgcg gcactgatct gtgcggtact gtagctgcca
     8101 tagcgactga gaaaaccccc gcaagagttc atcagacggt acgggacgtt cgagttggtg
     8161 atatttccgc catctacgcc tgttccgtac aggacatgta cagcctcatt gccgtaatct
     8221 ttcaggatcc gccgaagatt atcactgatg gtatccaggg cttcgtccca acttatccgt
     8281 tcaaatttac cttcaccgcg ctcgccgacg cgcttcatgg gatatttcaa cctgtcagga
     8341 tgattcatcc gtcggcggat agagcgcccg cgtaaacatg cccgaacctg atgattaccg
     8401 taaacgtcgt cacctgtcgt atcagactcc acccagtaca cggtgtcatc tttcacatgc
     8461 aaacgtagca gacagcggct cccgcagtta acggtgcagg aactccagac cgttttctct
     8521 tctaccggag cctctgccgc ccggaccatt tgggaaaatg gcagagtgaa agcactgctt
     8581 gccagcgcga gactgccaag tgcggaggtt ttcatcagac ttctatggct gatttcagcc
     8641 ttcatgagcg cctctgtggt atggattttc atcattactc acttattgct tttcaaacaa
     8701 aatgtcatgc cagaatttat ggttgtagtg ggttatattt tttcgatctc gaccagatta
     8761 gtgtgctgcg ggtttccctt cgccagtggt gaagggcgca gagtggttag cgtattcaca
     8821 catccgccat ggtcgatttt atcgccagac atattgggct cgtgccaggc tccctggccc
     8881 atagcgctaa ctccagggag aatgcgtggt gttactttgg ctggtagccg aacttcgcca
     8941 cgatggttaa acacccgcac catatcgccg ttggcaatcc cacgtttctg tgcatctata
     9001 gggttgatcc acacctcctg acggcaggct gccttcagga catcaatgtt gccgtaggtc
     9061 gagtgagtac gggatttgta atggaaacca aacagttgca gtgggaaggt tctacgttca
     9121 ggggagtccc agccttcaaa ggttgaggca taaactggca gtgggcttat cacttcatct
     9181 ttttccagtt cccaggtacg ggcaatttcc gccagcttgc tggaataaat ttcaatctta
     9241 ccggaaggcg ttttaagtgg atttgcctcg gggtcgtcac gaaatgcttt gtaggcgaca
     9301 aaatggccat tgggatcttt acgcttatag atacccattt ttttcagttc gtcgtaagac
     9361 ggtaacgccg gatctttggc aagcattttg gcgtacagat gttgtaacca ttgttcctgc
     9421 gtgcgacctt ctgtgaactt ttgatagacg tcaggtccaa gacgtttcgc gacttcactc
     9481 aggatccagt aaatcggttt acgttcgaat ttttcgctgg tgactggctg gaggaaaatg
     9541 agatatccca tgttaccggc gtagtcgtta ggaataatat cttcctgctc aacggtcatc
     9601 aggtcttcca gcagaatgtc ggcatatttt gccgatgagg tcataaagtt ttcgatgacc
     9661 acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa
     9721 tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccattgc cagacggatg
     9781 gatggacttg aagtagttga ctcaatgcta cttaaggtcc tgatatgact gacattaatc
     9841 atgataacac tgtatatcca tccagttctt tctctgggtc tccctatcgg tatcagcaag
     9901 tctacacctc aagccctgac acaaggctga cacaaatcaa tctactcagc gtagctaagc
     9961 ctcagttagt ccgcagagcc aatggtcgat atactatccg cttcagactc aagggacaga
    10021 ccacaccgtt tctttcagta tcgacccgca gcacagacag gagagtagcc acaatgcgtc
    10081 agagagagct tgcagccacc gctaaggcct tcatgttgga taggcctgaa gtctcactac
    10141 aggagcttac agagcatctc cggtctatgg ctgagcagtt cctgacagac gctagtgacg
    10201 attactggaa tggacttgag gtcgctactc tggtcgacga gaagtccaac ctgaaggagc
    10261 tagccgctac gcaagccttg agtctggacc agcagaaggg cataaggtta gcgctggaag
    10321 tcctgaccgc agcacagcag agagtcgaca ctggtgatac ctctggtctg attaagctga
    10381 ttgatgataa taaccttact gatgactcaa ctataggcga ctctacgtca attctgaata
    10441 atgagcaggg ggatagaccg gcagttttca cacaggagcg ccagtcatct gtagtttttt
    10501 caagcctagt gtcctcgctg ctggctgaga aggtccagac cctgaagaca tccagctaca
    10561 aggacctctc gtcctcactc aatactgtct cccggttcct gcctgaagac atggacctga
    10621 tgtcccgctc tgggtggctg gctgtcaggg actctatgct ggcttctgag gtgagaccat
    10681 cgacaatcaa caagctgctg accaaagcga agatgtgtct cgattatggc ctgatgaatg
    10741 ggcaacttga gggccgcaac cctattgaac ggatgaagct gaccaaggat atcgactcca
    10801 agcgcagagc gttcacagac gaggagctgg agagacttct ggtccgtgtt gagtctg
//
LOCUS       DBBBSB010000129        10770 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.129, whole genome shotgun sequence.
ACCESSION   DBBBSB010000129 DBBBSB010000000
VERSION     DBBBSB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.129"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(65..1909)
                     /gene="selB"
                     /locus_tag="ACIUS0_003408"
     CDS             complement(65..1909)
                     /gene="selB"
                     /locus_tag="ACIUS0_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGB0221022.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1906..3297)
                     /gene="selA"
                     /locus_tag="ACIUS0_003409"
     CDS             complement(1906..3297)
                     /gene="selA"
                     /locus_tag="ACIUS0_003409"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGB0221023.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3395..4003)
                     /locus_tag="ACIUS0_003410"
     CDS             complement(3395..4003)
                     /locus_tag="ACIUS0_003410"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGB0221024.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4074..5159)
                     /locus_tag="ACIUS0_003411"
     CDS             complement(4074..5159)
                     /locus_tag="ACIUS0_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGB0221025.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5162..5524)
                     /locus_tag="ACIUS0_003412"
     CDS             complement(5162..5524)
                     /locus_tag="ACIUS0_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGB0221026.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6061..7974
                     /gene="mtlA"
                     /locus_tag="ACIUS0_003413"
     CDS             6061..7974
                     /gene="mtlA"
                     /locus_tag="ACIUS0_003413"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGB0221027.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8069..9217
                     /gene="mtlD"
                     /locus_tag="ACIUS0_003414"
     CDS             8069..9217
                     /gene="mtlD"
                     /locus_tag="ACIUS0_003414"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGB0221028.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9217..9804
                     /gene="mtlR"
                     /locus_tag="ACIUS0_003415"
     CDS             9217..9804
                     /gene="mtlR"
                     /locus_tag="ACIUS0_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGB0221029.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9816..10025)
                     /gene="yibT"
                     /locus_tag="ACIUS0_003416"
     CDS             complement(9816..10025)
                     /gene="yibT"
                     /locus_tag="ACIUS0_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGB0221030.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10309..10671
                     /locus_tag="ACIUS0_003417"
     CDS             10309..10671
                     /locus_tag="ACIUS0_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGB0221031.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2600 a         2894 c         2762 g         2514 t
ORIGIN      
        1 gtgctttatt taaaatcata agaaatttgt attaatttat tttaaaaatt aattaatcat
       61 ttccttattt ttccggaaat aataatgcgt cgcgcaataa atgatcattt ccacgacgac
      121 gcgtaaagcc aatgcggtca aaatattcca gaatctgaat tgccagcttt cggcctacgc
      181 ctaagcgatc gcggaaatcc gccgcgcagg ttgaaccaca ctcctgatcg agatcgcgga
      241 tcatattggc aaattcgaca atccgatcgt tacggtaata acgatctttg acgatcgcgg
      301 taattattcc ttgctgcgcc gcctggcgta gcgtcaggcg cattgcctgc tcgtcggtac
      361 ccgtctcttt tgccagatca cgcacccacc acggctcgtc accaaacagc ggctctgctt
      421 tttgccagat ggcctgctgc tcttcgctga agcccgcttt gtgatccggc agatgcagcc
      481 aaccgtgatg actgaggatg tcgccgcttt cgcgcatctt ttcaatcagc aacagtacta
      541 gcgcttcatc ttccattggc aacgccatac gtcgcagacg ttcgcgccca ggcccaggtt
      601 catcgcgatg ttgctcatga taagtcgcta atgtgtcgag aattttccgt tgccagcgag
      661 cggcaaccgg cgcattcaac aagctataac cagcctgaat atagccaggc tgttgcagca
      721 gttcgcgcat cccttcgccg ttgagctggc gcgcccaagc gaaatcagga agattaaccg
      781 cgccgcgttg caggtgaacg gctaacgctt cgctatcatt ctgtgccctg gcgagggaag
      841 ccagccattg cagatactct ggtttacgtt taccgcgacg cggcgggtta agcatcacga
      901 cgcgcgctcc ggccagcgtg ttgcgggcag agatatcgcg caataccagg cggtcgttat
      961 ctgccagcca taacggggtg tcgaagacca gttcagcaag gttatcttcc agcagtgaaa
     1021 cgcgtcccgt gacgtggctg gcggcgtggt gaatatgcag cggctgccac tgggtcagcg
     1081 gtgcatgggt ttgaagctcg acaatcaccc gtgtgaacgg ctctggcggc acatcggcaa
     1141 gcagccagtc gccacggtta atctgctctt tttccgcatc acccgcgatg tcaagcgcga
     1201 tacgctgccc ggcattggcg gtttctgttg gctggttttg cgcatgcagc gcacgtacac
     1261 gcatcggttt atttacacca gtcagccaga gtgaatcgcc taccttcact tccccgctta
     1321 acgccgtacc ggtgacgacc agcccggcac cttttacggt aaatgcgcgg tcaatcgcga
     1381 ggcggaaact atgttggctg gcgtgctcgc gttccggcaa ctgaagcaga tgctcgcgca
     1441 gggcatcaat tccccgacct tcggttgctg cggtgataaa cagttttgcc tcagcaaaac
     1501 cgtattcccg cagaacctcc tttacctggc gttcaacctc atcaacacgc gcttcgtcca
     1561 cgcgatcggc tttggtcagc gccactgtca gcatcgggtt accggtcagc tgcaaaatcg
     1621 ccagatgctc acgggtctgt gccatcacgc cgtcatcgca cgccaccacc aacagcgcgt
     1681 gatcgatacc accaacgccc gccagcatgt tggaaagaaa cttttcatga ccgggaacgt
     1741 cgataaaacc aggcacgcga ccatccggct gcggccagta ggcatagccg agatcgatgg
     1801 tcatgccgcg ctttttttct tccggcagac ggtcagcatt tacgccagta atcgcctgca
     1861 ataaggttgt tttgccgtgg tcaacgtgtc cggcagtcgc aataatcatt tcaacaacat
     1921 ctccaaaaac cgttgctcat cttcaaggca gcgtaaatcc agccacaatc gtccgtcata
     1981 aatacgacca atcaccggca ctggcaattc acgccagcgg gcggctaatg actcaaggtg
     2041 gctaccgcgt ccatcatggg gtgtaaacgt taatgccgcg ctcggcaggc gatcaaccgg
     2101 cagcgaacca ctgccaatct gcgaaagaca tggcataacc tgtaccgcaa actccgcgcc
     2161 gtaatgtgcg acaaggggag cctgtaaacg ttgtgcctgg atttgaatga cctctgcgct
     2221 gcgggtaagc aggcgcaggg tcggtaattt ttcactcaga gcttcagggt gtaaataaag
     2281 acgcaacgtg gcttccagcg ccgcgagggt cattttatcc gcgcgtaatg cacgcttcag
     2341 cgggtggctt tgcaggcggg cgatcatctc ttttttacca acgataatcc cggcctgcgg
     2401 cccgcctaac aacttgtcgc cggagaagct caccagactg acgcccgccg caatcaactc
     2461 ctgcggcatt ggctctttcg gcaaaccgta ctggctaaga tcgaccagcg agccactgcc
     2521 taaatcagtc accactggaa catccagctc tttgccgagc gccaccagtt cagcttcgtc
     2581 tatcgctttg gtgaacccct gaatgctgta gttactggta tgtactttca tcaacagtgc
     2641 ggtattttca ttcaccgcct gacgataatc attcgcgtgc gtgcggttgg tggtccctac
     2701 ttcgtgtagg gtgcagcctg cctgacgcat aacatcggga atacgaaacg cgccgccaat
     2761 ctccaccagt tcgccgcgag ataccaccac ctctttcccg ctggcagtgg ccgccaacat
     2821 caataacacc gccgccgcat tgttattgac gatacaggca tcttccgccc ccgtaatacg
     2881 gcacagcagc tgcgccagcg cccgatcgcg atgtccgcgt ccggcgtcgt ccagatcgta
     2941 ctcgagggtc actggcgaac gcatagcctg cgcaacggct tccaccgcgg cttccgcctg
     3001 taaagctcgc ccaaggttgg tatgcagcac ggttcccgtc aggttgatca ccggacgcag
     3061 cgcgctctgc gcctctttcg tcaaccgggc atcgacttct tgcgcccagt tttcacacca
     3121 cgcaggcagc gtctggctgc cacgaatcac ttctcgcgct tcgtcgagca tctgacgcaa
     3181 caattccacc acgcgggtgt gaccataagt atcacgcaaa gaaaggaagg agctatcgcg
     3241 caataagcga tcaatagccg gaagttgact atagagggaa cgcgtttcgg ttgtcatagg
     3301 aaacctggct gatcaaggcc ctctcacacg gagaagggcg tttaacataa ccacggattg
     3361 taacgtgaga tgggtcagga ggacatatcg cgcatcaagc ctttggcggt tcggtgcgag
     3421 caaaactttc gcggctaaac aggttttcga ccagtttgac taagtgtggg cggtcgacac
     3481 accaacctgg cgcaacgcgg cggaaattga gatatccgac agcacaggca atggcgatag
     3541 tcgccagatt gaccgtatcg gttttgagtg tgccatcgac cagatatcct tccagcacat
     3601 ccagactgcg gttgattttc tcccgctggc gtaacaattc atcttcagac tgctgcgccg
     3661 ccggacgcgc ctgttcacgc accgatacca gcccggcatc cataatgcca tccgccagcg
     3721 cctcaatttt gcgcacccgc aacgactcca gcggatcgcg cggcaacatc gccggagcga
     3781 cattcattaa ctcaatatat tcagcgatga tcggcgaatc aaaccagcat tcgccctctt
     3841 cggtcaccag caccggcact ttccctaacg ggttaaattg cgccacgccg ttatccgcgt
     3901 tatagggcag ttcattaata aattcgaaag ttatgccctt ttctaacaac agaataaaaa
     3961 gtttgcgtac aaacgggctg gtgtagctac cgacgagttt catgccgagt cctttgtgcg
     4021 aggaaaaata tcagtatggc tcaggctcac gcaaaacaca ggtgatataa gtctcaatga
     4081 tccaaataaa gataatgcat ccaactggtc attcttagca gcactttccg catcaccgaa
     4141 acatggctga aatggtcgga gtaaaccgcc acctgggcgt agatgccgtc gggtaaggca
     4201 tcgatatcat cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggaacg
     4261 accgttaatg attgcaatac cccctgcgcc tgataagaac cgcctggcac gacaggtaaa
     4321 atactagtca atttgccgtg gaacacctgc ccaggtagcg cgttaaacac cacttccgca
     4381 tcatcgccag gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt
     4441 ttttgctcgg ggatgaagac catcaccgga cgcagcggca aggcagctgc gtatgtacct
     4501 gggcggatca gtacctgagt gacgtagcca ttgcttggtg cgcgaatgac agtctgctca
     4561 agattatatt ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg
     4621 ttgaccatac tgtcgagctg actctggatc tgcgcctgct ccgccaccga gcctttcact
     4681 aacgcatcct gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc cgagaacgga
     4741 ttcaccgccg cctggctgcc tttcaggtaa cgttgataat ttttaaacag acggtcgcgc
     4801 tccgctgaaa cctgggtggt gttggcctgc gcttcagtga gctgcgcacg cagcgtcttt
     4861 atattatgcg tcgccgtcat caggtcagcc tgaagtctgt caactcgcgc ctggtaacga
     4921 accgggtcga gcttaaaaag cacctcgccc ttttgaataa gctgattatt cttgtcagtg
     4981 acttcagtaa caattcccgt cacctgtggc gtgataggga tcgctatcac tgccttttgc
     5041 gcggtaaaag tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac
     5101 acgcctccca gcgccgccgt cgccagcgcc acgtaagtta aaacaatcaa tagatccata
     5161 attagatctc cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca
     5221 gccgggaaat tcggtcagaa agtgcggcga tttccggttc agtcgctttc tcctgcgacg
     5281 caacatgtga ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca
     5341 gaaacggcca gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt
     5401 cggcatgggg gtgattgcgc tttttggcaa tcagataagg gatgtcatgt atcgcgatga
     5461 ttccataaaa aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa
     5521 acatatctgc ctcggattca cgtttatcag tgttgttttt tggctggcag ccagaaggga
     5581 gtcaggctga tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat
     5641 aatttttaga ggtgatgagt tgcttagtta cataacgatt gtatgacgaa ggcataacat
     5701 gctgtagatc acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga
     5761 ttcaacaaac catcaaaaca aaaatgtgac attactcaca tttaaatgcc atttttagcg
     5821 aaaatcaccg ccttgtttct tttttataca ggcgttttgt gatgagcgtc acgtcaatta
     5881 cctctctacc ccctatattt atgtgactga tatcacacaa aagcccgtcg actggacagt
     5941 taaccgattc agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca
     6001 ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
     6061 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg
     6121 atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca
     6181 gggtggttac cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctccta
     6241 ccgctgctga tcggttatac cggtggtaag ctggtaggcg gcaaacgtgg tggcgtagtc
     6301 ggtgccatta ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct
     6361 atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt
     6421 aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
     6481 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg
     6541 ctggctgcgg gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt
     6601 gttgaaccgg cgaaaatcct gttcctcaac aacgccatta accacggtat cttctcgccg
     6661 ctgggtattc agcagtccca tgaactgggt aaatcaatct tcttcctgat tgaagctaac
     6721 ccaggtccag gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg tagcgctaaa
     6781 cagtctgcgg gcggtgcggc aatcatccac ttcctgggtg gtatccacga aatctacttc
     6841 ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc
     6901 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc
     6961 cttgctgtac tggcgatgac accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt
     7021 gcggcgatgg ctgtctcctt cgttgtctct gctattttgc tgaaaaccag caaagtgaaa
     7081 gaagaagatg atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa
     7141 ggcacatctc cgctatctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc
     7201 atcgttgcct gtgacgccgg tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag
     7261 aaaattcagg atgcaggtct gtcgcagatt tcggtcacta acagcgcgat caacaacctg
     7321 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag
     7381 gttccgcagg cacagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc
     7441 ctaaccgaac gtctcgttgc tgcccagcgc catactgaaa acgaagtgaa agtgaaagac
     7501 agtctgaaag acagctttga cgattccagt gctaacctgt tcaaactggg cgcggagaac
     7561 atcttcctcg gtcgcaaagc agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag
     7621 ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa
     7681 ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa
     7741 gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt
     7801 gaagaagaag atgacattgc ccgtctggtg attggtattg cagcccgtaa caacgagcac
     7861 attcaggtta tcaccagcct gaccaatgca ctggatgatg agtccgtcat cgagcgtctg
     7921 gcacacacca ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat
     7981 cctaccctct cctcacggag agggtttggg tgagggaaaa gcctcacccc agccctctcg
     8041 ggtaaaaaca ttgatgaagg ttaatactat gaaagcatta cattttggcg caggtaatat
     8101 cggtcgtggc tttatcggta aactgctggc agacgcgggt atccaactga cgtttgccga
     8161 tgtcaatcag gtggtacttg atgccctgaa tgcccgtcat agctatcagg ttcatgtggt
     8221 cggtgaaacc gagcaggtag ataccgtttc cggcgtcaat gctgtcagca gcattggtga
     8281 tgatgtcgtt gatctgattg ctcaggtcga tttagtcact accgccgttg gcccggttgt
     8341 gctggaacgt attgctccgg ctatcgccaa agggctggtg aaacgcaaag aacaaggtaa
     8401 tgaatccccg ctgaacatca tcgcctgtga aaacatggta cgcggcacca cgcagctgaa
     8461 aggtcatgtg atgaacgccc tgccggaaga cgccaaagcg tgggtagaag agcacgttgg
     8521 ctttgtcgat tccgccgttg accgcatcgt accgccttcg gcttcggcaa ctaacgatcc
     8581 gctggaagtg acggtagaaa ctttcagcga atggattgtc gataaaacgc agttcaaagg
     8641 cgcactgccg aacatcccag gcatggagtt aaccgacaac ctgatggcat ttgtcgaacg
     8701 taaactcttc accctgaaca cgggtcatgc tataaccgcg tacctcggaa aactggccgg
     8761 tcatcagacc attcgtgacg ctattctcga cgagaaaatc cgcgcggtgg taaaaggtgc
     8821 gatggaagaa agtggtgcgg tactgatcaa gcgctacggc tttgacgcag acaaacatgc
     8881 ggcgtacatc cagaaaatcc tcggtcgttt tgagaacccg tatctgaaag atgatgtaga
     8941 gcgcgtaggc cgtcagccgc tgcgtaaact gagtgctggc gaccgtctga tcaagccact
     9001 gctcggtacg ctggaataca gccttccgca caagaatctg attcagggga ttgctggtgc
     9061 aatgcacttc cgcagtgaag atgatccgca ggctcaggaa ctggcagcac tgatcgctga
     9121 caaaggtccg caggcggcgc tggcacagat ttccggtctt gatgccaaca gcgaggttgt
     9181 atccgaggcg gtaaccgctt ataaagcaat gcaataatgg tggaccaggc gcaggacacc
     9241 ctgcgcccga ataacagatt gtcagatatg caggcaacaa tggaacaaac ccaggccgtt
     9301 gaaaaccgtg tgcttgagcg tctgaatgct ggcaaaaccg tgcgaagctt tctgatcacc
     9361 gccgtcgagc ttttgaccga ggcggtaaat cttctggtgc ttcaggtatt ccgcaaagac
     9421 gattacgcgg tgaagtatgc tgtagaaccg ttactcgacg gcgacggtcc gctgggcgat
     9481 ctttctgtgc gtttaaaact catttacggg ttgggcgtca ttaaccgcca ggaatacgaa
     9541 gatgcggaac tgctgatggc attgcgtgaa gagctaaatc acgacggcaa cgagtacgcc
     9601 tttaccgacg acgaaatcct tggacccttt ggtgaactgc actgcgtggc ggcgttacca
     9661 ccgccgccac agtttgaacc agcagactcc agtttgtatg caatgcaaat tcagcgctat
     9721 caacaggctg tgcgatcaac aatggtcctt tcactgactg agctgatttc caaaatcagc
     9781 ttaaaaaaag cctttcaaaa gtaagcaacg tctgcttact gcccctctac ctgcttcggc
     9841 cgataaagcc gacgataata ctccagacgt tgtagatata acggcacgct ttcatcgggt
     9901 attccggacg gaatcgcgtt acggggagga agttttttca gatactcccg gaacgcctgg
     9961 cttgatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
    10021 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt
    10081 aggttcccgg taaagatgac attaaggaaa acgtgctgaa tcctcaataa tcatccgctg
    10141 ctgtttatta tctttttcta tagatttctt aattaatcaa cgaattgcgt agtttttgag
    10201 catgttaatg attacgttgc agaactatta caatgtcatc agttgtgaac aaagcacctg
    10261 gtcgcgcata ctaggggcta taaatttatc tttatcagaa gccatcacat gaaagaagtc
    10321 gaaaaaaacg aaatcaagcg tctcagcgat cgcctggacg ccatccgcca ccagcaggcc
    10381 gatctgtcgc tggttgaagc cgcagacaaa tatgccgagc tggaaaaaga gaaagccacg
    10441 ctggaagcag aaattgctcg cctgcgtgag gttcatagcc aaaaactgag taaagaagca
    10501 cagaagctga tgaagatgcc gttccagcgc gcaattacca aaaaagagca ggctgatatg
    10561 ggcaagctga agaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggg
    10621 cgcgaaatgg gcctggaaga gatgactgga ttttctaaga ctacgtttta agcccctcag
    10681 acgactggtt cgatcgtatc aacccgcatc tggcttgcct tagctggtca atgcggggac
    10741 tctcctgtct actcgcctaa ttaccaaaca
//
LOCUS       DBBBSB010000130        10760 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.130, whole genome shotgun sequence.
ACCESSION   DBBBSB010000130 DBBBSB010000000
VERSION     DBBBSB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.130"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            186..836
                     /gene="csgD"
                     /locus_tag="ACIUS0_003418"
     CDS             186..836
                     /gene="csgD"
                     /locus_tag="ACIUS0_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGB0221032.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            841..1230
                     /gene="csgE"
                     /locus_tag="ACIUS0_003419"
     CDS             841..1230
                     /gene="csgE"
                     /locus_tag="ACIUS0_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGB0221033.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1255..1671
                     /gene="csgF"
                     /locus_tag="ACIUS0_003420"
     CDS             1255..1671
                     /gene="csgF"
                     /locus_tag="ACIUS0_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGB0221034.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1698..2473
                     /locus_tag="ACIUS0_003421"
                     /pseudo
     CDS             1698..2473
                     /locus_tag="ACIUS0_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2537..3028)
                     /locus_tag="ACIUS0_003422"
     CDS             complement(2537..3028)
                     /locus_tag="ACIUS0_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGB0221035.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACIUS0_003423"
     CDS             complement(3130..3684)
                     /gene="ycdY"
                     /locus_tag="ACIUS0_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGB0221036.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACIUS0_003424"
     CDS             complement(3708..4445)
                     /gene="ycdX"
                     /locus_tag="ACIUS0_003424"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGB0221037.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACIUS0_003425"
                     /pseudo
     CDS             complement(4500..5438)
                     /gene="ghrA"
                     /locus_tag="ACIUS0_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5673..5760
                     /locus_tag="ACIUS0_003426"
     tRNA            5673..5760
                     /locus_tag="ACIUS0_003426"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5707..5709,aa:Ser,seq:gga)
     gene            complement(5910..6191)
                     /locus_tag="ACIUS0_003427"
                     /pseudo
     CDS             complement(5910..6191)
                     /locus_tag="ACIUS0_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(6473..7477)
                     /locus_tag="ACIUS0_003428"
     CDS             complement(6473..7477)
                     /locus_tag="ACIUS0_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HGB0221038.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(7549..8109)
                     /locus_tag="ACIUS0_003429"
                     /pseudo
     CDS             complement(7549..8109)
                     /locus_tag="ACIUS0_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8194..8391)
                     /locus_tag="ACIUS0_003430"
     CDS             complement(8194..8391)
                     /locus_tag="ACIUS0_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGB0221039.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(8403..8894)
                     /locus_tag="ACIUS0_003431"
     CDS             complement(8403..8894)
                     /locus_tag="ACIUS0_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGB0221040.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(8891..9265)
                     /locus_tag="ACIUS0_003432"
     CDS             complement(8891..9265)
                     /locus_tag="ACIUS0_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGB0221041.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(9312..9686)
                     /locus_tag="ACIUS0_003433"
     CDS             complement(9312..9686)
                     /locus_tag="ACIUS0_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGB0221042.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(9849..10070)
                     /locus_tag="ACIUS0_003434"
     CDS             complement(9849..10070)
                     /locus_tag="ACIUS0_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGB0221043.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10133..10609)
                     /locus_tag="ACIUS0_003435"
     CDS             complement(10133..10609)
                     /locus_tag="ACIUS0_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGB0221044.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(10625..>10760)
                     /locus_tag="ACIUS0_003436"
     CDS             complement(10625..>10760)
                     /locus_tag="ACIUS0_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGB0221045.1"
                     /translation="AACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID
                     "
BASE COUNT         2769 a         2636 c         2604 g         2751 t
ORIGIN      
        1 aacacttgat ttaagatttg taatggctag attgaaatca gatgtaatcc attagtttta
       61 tattttaccc atttagggct gatttattac tacacacagc agtgcaacat ctgtcagtac
      121 ttctggtgct tctattttag aggcagctgt caggtgtgcg atcaataaaa aagtggggtt
      181 tcatcatgtt taatgaagtc catagtattc atggtcatac attattgttg atcactaaac
      241 cttctttgca ggcgacagct ctcttgcagc accttaaaca atcgctagca attacaggaa
      301 aattacataa tattcaacgt tctctggacg atatctcttc aggctctatt attcttctgg
      361 atatgatgga agcggataaa aagcttatcc attattggca ggatactttg agcaggaaaa
      421 acaacaatat caaaatattg ttgctaaata cgcctgaaga ttacccgtac cgcgacattg
      481 aaaactggcc tcatatcaac ggcgtttttt atgccatgga ggatcaagaa cgtgttgtca
      541 atgggttgca aggcgtcctg cgtggcgaat gctactttac gcaaaagctt gccagttacc
      601 tgattacaca ttctggtaac tatcgttata acagcacgga atcagccctc cttactcatc
      661 gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc taataacgag atcgctcgtt
      721 cgttgttcat cagcgaaaat acggttaaaa cgcatcttta taatcttttc aagaagatag
      781 ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga taacctcagg cgataaagcc
      841 atgaaacgtt atttacgctg gattgtggcg gcagaatttc tgttcgccgc agggaatctt
      901 cacgccgttg aggtagaagt cccgggattg ttaactgacc atactgtttc atctattggc
      961 catgattttt accgagcctt tagtgataaa tgggaaagtg actatacggg taacttaacg
     1021 attaatgaaa ggcccagtgc acgatgggga agctggatca caataacggt caatcaggat
     1081 gttattttcc agactttttt attcccgttg aaaagagact ccgagaaaac tgtcgtcttt
     1141 gcactgattc aaactgaaga agcactaaat cgtcgccaga taaatcaggc gttattaagt
     1201 acggacgatt tggcgcatga tgaattctaa ataaaaaatt gttcggaggc tgcaatgcgt
     1261 gtcaaacatg cagtagttct actcatgctt atttcgccat taagttgggc tggaaccatg
     1321 actttccagt tccgtaatcc aaactttggt ggtaacccaa ataatggcgc ttttttatta
     1381 aatagcgctc aggcccaaaa ctcttataaa gatccgagct ataacgatga ctttggtatt
     1441 gaaacaccct cagcgttaga taactttact caggccatcc agtcacaaat tttaggtggg
     1501 ctactgtcga atattaatac cggtaaaccg ggccgcatgg tgaccaacga ttatattgtt
     1561 gatattgcta accgcgatgg tcaattgcag ttgaacgtga cagatcgtaa aaccggacaa
     1621 acctcgacca tccaggtttc gggtttacaa aataactcaa ccgattttta agccccagct
     1681 tcataaggaa aataatcatg cagcgcttat ttcttttggt tgccgtcatg ttactgagcg
     1741 gatgcttaac cgccccgcct aaagaagccg ccagaccgac attaatgcct cgtgctcaga
     1801 gctacaaaga tttgacccat ctgccagcgc cgacgggtaa gatctttgtt tcggtataca
     1861 acattcagga cgaaaccggg caatttaaac cctacccggc aagtaacttc tccactgctg
     1921 ttccgcaaag cgccagggct tacaaaacct gcttaacgag cgcaaggtta ttcgtgcggc
     1981 acaagaaaac ggcacggttg ccattaataa ccgaatcccg ctgcaatctt taacggcggc
     2041 aaatattatg gttgaaggtt cgattatcgg ttatgaaagc aacgttaaat cgggcggtgt
     2101 tggggcaaga tattttggga tcggtgcaga cacgcaatac cagctcgatc agattgccgt
     2161 gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc tcctcggtga acaccagtaa
     2221 gacgatactt tcctatgaag ttcaggccgg ggttttccgc tttattgact accagcgctt
     2281 gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt atgctgtgtc tgatgtcggc
     2341 tatcgaaaca ggggtcattt tcctgattaa tgatggtatc gaccgtggtc tgtgggattt
     2401 gtaaaataaa gcagaacggc agaatgacat tctggtgaaa taccgccata tgtcggttcc
     2461 accggaatcc tgacagataa aaatgccgga attaataatc cggcatttac tgtttaagcg
     2521 aaagcgtgag tgcccctcac gctttatttt tgatttcctg ctcacggtga gccgtctttg
     2581 cactacgcgc cgccagccac aggccgctgt ttttcattgc gtaaccaaat accagcccaa
     2641 gcgccagcga aggtaacacc agtttccagt caccctgccc ggcaaacgtc gcgcatgcgc
     2701 ctataaacgt ccccggaaca aatgaaagca gcagctgttt ggcctgaata cacatcagaa
     2761 acgcgacaat accggttatg acataaccga gaatttccag atgtggtgcc agtgcactac
     2821 cgtaaataat gaccatcgcc cacaccacgc cactaagcag ggttgcagcg gagatcgcca
     2881 gccctttcag cccaccttgc gggcaagcaa agtaggccgt acagcccagg aagcccgccc
     2941 agcttagtaa gcccagggaa acagccaccc atccccagat accggagaga atgcctgttg
     3001 tgattgcaat agagagaagt atgttcatgc cgcgcatgat agcagaaaac gcggccatga
     3061 atgagatcga gcacacattt tatgcaacaa caaattatct gttgcagtta tgagtgacga
     3121 tgatcacatt tactcttcag aatcttcttc cagctcgtcc cacattgcac taatggcatc
     3181 acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt gcatgggcct ccactttgcc
     3241 gaggaacgca ccacaccagg gtaacagata ctcactgaac agtgtttcca gtgcttcgct
     3301 ctcatcttcc gttgactgat cttccagcca ggaagccgca agcagcaatg tgccgatgtg
     3361 atcggctggc gtatccgcca gtagcatccc tcgttcggaa agaaaagcgc gcacttccgc
     3421 ttccgttgcg tcctcaaccc atgcgctacg atatggcggc acggcacatt catcgccgat
     3481 aaacaacgca ttgtaatcgg cagagacttg cgtcatatca cagcttttct gtaaacgcgt
     3541 cagtaactca tcctgctcca gtggccagtt cgcagccagt ttcccctcac gaatcagagt
     3601 aaacagcggc accagtaaag gatcttgcgg ttggcggtaa tagagcgaac ccagcacacg
     3661 acagaggata gaaaactcgt tcattaatat atttccatta ctagtaatta aaggtctgca
     3721 aattccgcaa tcggtgccat accgcgagat tcaaggaagt tcagtaagcg gcgcggagaa
     3781 acattcaaaa tgcgctctag cggaaaatct accgcgtcga ggattttaag acactcttca
     3841 aattccccca tggtaaacgc ggtgtgagaa tccgagccta atgccaccca acctccagca
     3901 tcacgtaccg ctgcagctac cgcacgacag ttgtcttcac tgcccttacg tgagtgtaaa
     3961 aatgaggaat tattgatttc cagcgccacc tgatgtttcg cggctgcttc agcaacggct
     4021 ttaacatcta tttcatattt cggatttccg ggatggctta ttatatgcac attgccgctg
     4081 gcgatagtgg agatcatcgc ttgtgtattg gtcgctttgt catgtggcgc aaaaaccggc
     4141 tcatgaaaac cggcaataat cagatccagc gagtcaaaca ttttaccgct gcagtcaatt
     4201 tcaccatcaa catttttaat gttagcttca atgccgcgca ggatccctac cccatcaacc
     4261 actcgcggcc agatacgcat gttaatgaag tgccagtgat gcggcgcatc ttccatatcc
     4321 gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg ggcaatgtaa
     4381 tcactcaatg tgctatatgc atgtgtgctg gcaacggtat gcatatgaag gtcgacggga
     4441 tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct gatgctttat
     4501 tagtagccac gtgcgcggtc gacttgcccg cagaccctct cccctttttc gagctgggca
     4561 atagtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc gacatgtggt
     4621 gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg ttcacgatta
     4681 aaaacatcca gcattgcgcc tttaactttc ccgctatcca gcgccgcgag caggtcatct
     4741 tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatcagg taatttttcg
     4801 agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa attaatcaat
     4861 acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct ttgcacgcca
     4921 ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgctaggt ttgcagactc
     4981 tgagcaactt tacagcccag tacgcctgcg cccaaaatgc cgatggtaaa atcttcccga
     5041 tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata atcgtcaaaa
     5101 cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc gcccataccg
     5161 gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg tgcctgtagc
     5221 ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag atcgcgccct
     5281 gccagcattt caacaggagg atgccagact aaaacataat cagcagagtc attatctccg
     5341 cttttccatg ccctgactct tgcctgagga atagctttgc gcagtgcctc aatccaccat
     5401 tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga aaagcgttgt
     5461 gcgaaattta ttcgcactta tcgttatgat ctacaaaggc caccagcata acaaatccgt
     5521 ggtcggtggc aaaaaaagca gatttcgctt attaaaacca cacattgatt gaatgtttga
     5581 ataaacgcgc gattttttca aaaagtttgt tgacctcagg tcatgatttc cctaaattag
     5641 cgcccgttcc agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc
     5701 acgcctggaa agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca
     5761 gattataagt gcatgttatt gtttaacatg cgctttttta tttttccttc cagacagtat
     5821 gccatcgggt atgccatctg aaaccggttt atatgccccg cgatcatatc ttatcggaca
     5881 atttccgatc tttgagcata ctggcaaagc tatactgcca gcatatcagg atagtgtttt
     5941 gcgataatat cattcagtct gtcaaccagt tctggttttc tgaacgtgat atgcgcggag
     6001 cccttcttaa agtatctgat gctgaacatc tcatcttcat agctctcttt accctgaaca
     6061 gactgaatgt gatcatccag acgaagggtg atgtttcccc ggttgtcggg gattggttta
     6121 ccgctgaaca ggtgcaacat tctttccaga tcggcgagtc ggtcagcctg ttgtccggtg
     6181 ataagatgaa atctatggtc acccccgttt ttgcaacact gatttttgat taatggttgg
     6241 cttgcttaaa tctatccggc gtctctgtgg gcaaggatgc ccgcgccccg atgagttccg
     6301 cacctgataa tcctaaaaat cccctcggct tcagtgagcc atttcatctg ttaggttttt
     6361 aatcaggtcg atttgccgtt catgtcatca ataatcagtc ttcgcaaaac cagaaaggta
     6421 acttgtatct tattgtttaa aataaactaa ttgagttata tcagcatcta tatcaggcct
     6481 gatattcttt tttctgagta gtgattgccc aggcaattcg agccagttta tttgccagag
     6541 cgcaggtaac cacattcgaa tgcttcttgt tgagttgctc ccgaacccac tcagcaagtt
     6601 ttccatgctt gcgttcaagt cgcattatga atgagcgggc acactgaacc agtaaccggc
     6661 gtaaattttt atcacctcgc ttactgatac ccaacagagt cgattttcct ccagtgctat
     6721 actgccgggg aacaagtcct gtggaagctg caaaatccct gctgcatgag aactgtttcc
     6781 cgtcaccaag ttgcgacgat agtaagctgg cagtaatcgg accaacacca ggaattgtca
     6841 taatgcgctg agcggtatca tcagcgttga ttgactgact taattctgat tccagctctg
     6901 ctatttgctc aacaaggtaa aagtagtggg tatgcaacct caccaaaagg cggctcagat
     6961 attcggggat ttcgtgttca gccaacacga gagacaaccg ttttattaca gcatcaccta
     7021 tcggcaggct gattccaaat tccagcaaga aaccgtggat ctggttggtt gttttcacct
     7081 tatctctgat gagcgattct ctgacccggt gtaacgccct catggcctgc tgggtctcag
     7141 ttcttggctg gacaaaacgc attgatggtc gtgaagccgc ttcgcatatg gcttctgcat
     7201 caacaaaatc attcttatta cttttgacaa acgggcgaac gaactgtgga gaaatcagct
     7261 tagtttcatg cccaaaatca gagatacagc gagccataaa gtgtgcaccc gcacaggctt
     7321 ccattacaac aactgagggc ggacatgtcg ccagaaactg catcagttta gtacgggtaa
     7381 attttttacg cagaagagcc ttaccagact tgtcttgaca atgaatgtgg aaggagtgct
     7441 ttccgagatc gataccaata agtgtgatgt tctgcatgat ggtctctccg ttaaagggat
     7501 ttaccttaac agcgtactgc tgttaaggtg ggggtgacca tatcattatc cccaccggtc
     7561 ccatctcacc agattgttga cgataatttt actgccaaat ttacaggggc aattggtttt
     7621 gtaattccag ttcagccctc tgaagacgtt gatcacccct cgctcaaaca cctcatcctt
     7681 attctggtgc agttgttcaa atgtgctcag aatgttcgct tcgctgattt ccggaaaatt
     7741 atcgtactcc agtgaccggt accatgtatc acgggcctgc gtatccatta atgacagcat
     7801 cccggaacgt tgcatcaggt cacgccagat ttcgcgatca agattccggg taatggcttt
     7861 cattgctgtt tcgggtttct ccatcagcca gcagccgcag cgaaaatcct gtttcatggc
     7921 ccagtccggg gcagttttac ctccgatgct ttccgtcagc accgagattt cagccagttg
     7981 tcggataagt gtgtcgattt gcaacagtgt actctttcgt acagccagaa tgtggcgaat
     8041 gctggttgag cagataacgt ccgtatgccc gttgaccact tccggacggg agattgtgat
     8101 atctgacata atcgatattc ctttttgcag ataaatgaaa acgccagccc tgtgcaggct
     8161 ggcgtcagag gaatgaagtc tggtgagtga gcttcaccgg ttctggtgca gaagctgcag
     8221 atgacgcagg gtacggacat cctcccgggc ctgttcgata cagggcaaca gtgctgctga
     8281 atccgtttta tcctcatcgt tgtcaaagat aattcccgat ccgcagtcga tattgtcctg
     8341 cagccacgca atcagaatat ccagcgctgt ttccgtggtt aatgatttca tgttgttcct
     8401 ggttaccagt cgaaagtggg taaacccggc aggcattcag cgctggcatc cagatgaatg
     8461 agactgacgc cataacgccg gatgagtgtg acgaccagac gacggaacgt ttttgacagc
     8521 cccaggcgtt taagccgcag aaccgggtat gaccacgcat cggtacgtaa cagatatccg
     8581 ctacctgtaa aatgaatcca ttctgattca ccgaagtcac tggtctggtg tgacagcgag
     8641 tacaaccagg cgttgtccgt ttccgtgata tgtgcggtac tgcagcgtat gccggtaagg
     8701 gtagcaaatg gtggcgtgca ttctgctgag gtctggtcag attcatcaac aacacgaagg
     8761 gagtagccgt tatcgcagac catgttaatc agttcagcga gctcaatacc atcgacgtca
     8821 acgacaatgc gccctatgtt cagtgcctgt acgttaacgc tgtcggcttc cagtgtcagg
     8881 gccagtttca tcatttcgcc tccggatact tacccagggt aatgttattt accgttctgt
     8941 aattgtcgcg ggtcatcagg ccggtcgccc ggcgagcccg gaggatatca atactgttta
     9001 ttaactgaga gcggggacag gtgctgaatc ccggctggtc ggtacgcacc agcgcgtatt
     9061 tttccacgag aaaattaact gcatcacaca gtgaaatgcc tgcctcaata tgctgctcaa
     9121 tcacacgttc atcgacaaac ggtgtgtcat tcagtgtgag gccataatgc tggtccagca
     9181 gtcggatgag cagtgtctgc cagatggtga cgggagacgg gcagcgagat acctcccgta
     9241 catgtgtgtc ggataatgtt ttcatcttaa gttctctgta actggtagtg ctaaaggtga
     9301 gagcttcggg gttatgaggt ggttgcgggt gctggtgtcg gataaacagc cagataaacg
     9361 taaccacagg agccgagggt gtcggcttcg caggtcagcc cttttgcata cagcgtgacg
     9421 gtatgctgat atcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc
     9481 agcagcggaa aggcctggtc cagatggtat gcatctgcgt cgctgaagcg gcctctgata
     9541 ccggcgcggt cggcaaggta atacaggcgg ttaccctctt gcaccagacg agcgccaaaa
     9601 cagggcctca ctgtgcaggg aagcccccac caggggcggt cgtgattgtc atcgggatga
     9661 gttatcccgg agagtttgtc tgacacgata aaatcctctc tgaaaagtgg ttaagaatgg
     9721 tttaacgatg acaggtcagc cggcgcgctg accggtttcg gtgaagatgt atgcattgat
     9781 gagaatggcc tcatccacag cggcgtctga ggtcatctgg tcgtactcgt ttcccgggtg
     9841 acggaacatc agttcagggt gacactcatc agccggacat acgggccaaa actgtcctta
     9901 cggcgttctg caaacacggc aagaacgccg ggaatatcct gcacttcacg accagtatac
     9961 gcttcagcac tgccgtgcca gcgatactta ccggtacaga acggaaaaag acgggatgca
    10021 ggatgctgtt ggtgaatacg catggcttca ccacgggtga tgattctcat gatgtgatac
    10081 ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt ggttgtgacg ggttaaagca
    10141 gaccatgttc ggcaaaggag aaaacctggt tgccaccgac tatcagatgg tccggcaccc
    10201 ggatatccac caggcccagt gcctgtacca gacgttcggt gataagccgg tctgccttac
    10261 tgggtgtgac ttcaccggac gggtgattgt gcgccagcac cacggcagcg gcattgtggt
    10321 acagggcgcg tttaatcact tcccggggat gaacttccgt acggttgatg gtgccggtga
    10381 agagggtttc accggcaatc agctgattct ggttgttcag atacagcacc cggaactctt
    10441 cccgctccag tcccgccatg ttcagaatca gccattcccg tgccgcacgg gtggaggtga
    10501 aggccacgcc gggttcatga agatggcggt ccagggtttg cagggcccgc agaatgagac
    10561 tgcgctcgcc gggcgtcatg tttccgggca gaaaggaaag ctgctgcatt gtccctgcct
    10621 ccattcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc
    10681 gcagccggta ataatggacc gtcatggcat aacactccgt acgacaggca tgatgactgt
    10741 acgtcatcag acaggcagca
//
LOCUS       DBBBSB010000131        10650 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.131, whole genome shotgun sequence.
ACCESSION   DBBBSB010000131 DBBBSB010000000
VERSION     DBBBSB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.131"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            9..839
                     /locus_tag="ACIUS0_003437"
     CDS             9..839
                     /locus_tag="ACIUS0_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGB0221046.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(980..1753)
                     /gene="uxuR"
                     /locus_tag="ACIUS0_003438"
     CDS             complement(980..1753)
                     /gene="uxuR"
                     /locus_tag="ACIUS0_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGB0221047.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1968..3428)
                     /locus_tag="ACIUS0_003439"
     CDS             complement(1968..3428)
                     /locus_tag="ACIUS0_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGB0221048.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3509..4693)
                     /gene="uxuA"
                     /locus_tag="ACIUS0_003440"
     CDS             complement(3509..4693)
                     /gene="uxuA"
                     /locus_tag="ACIUS0_003440"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGB0221049.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5033..6376
                     /gene="gntP"
                     /locus_tag="ACIUS0_003441"
     CDS             5033..6376
                     /gene="gntP"
                     /locus_tag="ACIUS0_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGB0221050.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6627..7529)
                     /gene="fimH"
                     /locus_tag="ACIUS0_003442"
     CDS             complement(6627..7529)
                     /gene="fimH"
                     /locus_tag="ACIUS0_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGB0221051.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7549..7992)
                     /locus_tag="ACIUS0_003443"
     CDS             complement(7549..7992)
                     /locus_tag="ACIUS0_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0221052.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8065..8595)
                     /gene="fimF"
                     /locus_tag="ACIUS0_003444"
     CDS             complement(8065..8595)
                     /gene="fimF"
                     /locus_tag="ACIUS0_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGB0221053.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8605..>10650)
                     /gene="fimD"
                     /locus_tag="ACIUS0_003445"
     CDS             complement(8605..>10650)
                     /gene="fimD"
                     /locus_tag="ACIUS0_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGB0221054.1"
                     /translation="NRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKN
                     KWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAP
                     VIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQI
                     FTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQ
                     LADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTN
                     IQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQL
                     TVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKG
                     RDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNL
                     SYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHA
                     NGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTN
                     TLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQ
                     SSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2540 a         2765 c         2636 g         2709 t
ORIGIN      
        1 taactattat gtctatgcca ttaagcaatg ccgctaataa ccaattcaat acaaataatc
       61 acttccttca tcatccaaaa gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc
      121 tggatacgga aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg
      181 gaaaaagcgt tgtcgaaatt agaaaactta atattggcaa acagtcctgg ccatttaatt
      241 atgtgacaga tacatgcaga gaattcaatg gtattacaac aacaggacgt atgctctatc
      301 gaaacttgaa aatcacctca gctttagacg aaatatatgg tggtatatgt aaaaaggcgc
      361 atgctgctac agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg
      421 atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc aatgttcctg
      481 gttatgccgg aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat
      541 ggaccaatga gcagccaaga attgatatct atattgatca gataataact gtctcagggg
      601 tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa
      661 atgacccaat cattggtata gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg
      721 gatataacgt tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt
      781 catctcttcc agaactgttt gaattaaaaa atggtgaatg gaattatatt ggtaaatagc
      841 cactgagaag aatgagtact aatattcagg ttatacagat atttaaacaa aaccctgcaa
      901 tttacaaagt aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc
      961 ttgcgcttat tataataagt caggcgtcga ctttatccag cggccatgaa tcaaagagat
     1021 agccgtcaaa ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt
     1081 ccagatgctg ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga
     1141 ggatctgttt gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt
     1201 gcaactggat ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca
     1261 ccagcatgct gttatgcgtt gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt
     1321 cgctgctacc cggcgcgctg gaagccagtt cacgctcttc cagttgcaat gcctgacgca
     1381 ttttgacgat atcttcgcgg gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca
     1441 gtaactgacg cgcctgtaac agctcaaaag gacccgcatc attgcagacg ttggcatccg
     1501 gactgtctgt gttgtggctg cctgagctat caagcacata gataccggca ccccggcgca
     1561 cttccaccag ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga
     1621 catcaagcat ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg
     1681 tctttatgat cagatcgtgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg
     1741 tgacagattt catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg
     1801 gcgtgaacgc cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact
     1861 ataccattag cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac
     1921 caaatatcag tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc
     1981 acacactcgc gcgcaccgcg ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca
     2041 aagtcggcat tctgcggcag atcatcggca aaaataccgc tcaggcccag cagcgctttc
     2101 acgcggtctg cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg
     2161 accacgtcaa tggcattacc ctgctcatcc acgccctggg tgtaacccat ccagccagcc
     2221 acgcccagcg ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc
     2281 agtagacgct gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc
     2341 agagacgggt tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct
     2401 tccggcatcg acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga
     2461 taatccgggt tagtcatggt gtcggcaatg gtttcatagc cgccgaggta accgaggtac
     2521 gccaggaaag agtggctgcc gttcagcata cgcagcttca tcatttcgaa cggcacaacg
     2581 tctgcaacga actgtgtgcc cactttatcc cagtccgggc gaccattaac gaagttatct
     2641 tcaatcaccc actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc
     2701 tggtcagcaa tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg
     2761 gtgcacggga aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc acgcgcctga
     2821 gccagcccca gtaccgcgac cttcgccaca tggccgtttt cacgcacgtt atcgcaggac
     2881 atcaccgtaa atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa
     2941 ccaatcgcgg acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg
     3001 agatccagtt gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgttagagag
     3061 acaatcgccg tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc
     3121 ggatgcagcg cttctttcat cgaaccgata attttcagct cggtgctttc tgcgcctttt
     3181 tccgctacgg tgtacagcag ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg
     3241 cctggcatca ggttaacttc gcagatgccc cagtcgctgt cggtgctttc cagcagatga
     3301 tgggtataca gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt
     3361 gattccagac gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata
     3421 gtagtcatcg taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg
     3481 gcacggcgcg cagccatgcc ggtgggtatt aacggataaa gaaagcgcgc tggatcgcca
     3541 gttcgacacc gcgaacttcg gccaggcctt tcagacgacc aattgcggag taaccagggt
     3601 tggttttctt cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca
     3661 ggtcttcttt gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt
     3721 tgtacatatc aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt
     3781 cacgcatggt ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat
     3841 caaccagatc gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg
     3901 ccatgctgtt tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg
     3961 gcaagccgag gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc acgccaactt
     4021 cttcagcaac tggaataatc gctttcagga agacggcaaa gttttcgcgc agtttggctt
     4081 tgtcgatatc tttgtacagc tccaggtgct tacggaactg gtcgagggta tacccttctt
     4141 ccgcgcccgg cagaccggca atgatgttac gcgtcagacg cgctttgtct tcgtcgctca
     4201 tagtggcgaa gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc
     4261 ctggacgttt caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag
     4321 ctttggagcc gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca
     4381 tgaagttgta gcacacggtg cgaatgccac actgcgccag gttgcgcaga gtctgctgat
     4441 agttagcgat ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta
     4501 cgctttctac gacagaccac accaggcctg cgtcttcaac gatcgccttg cgtttgagga
     4561 tctcttctac ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag
     4621 ttgcgcccgc ctgacggaca tcagctaaag aaaccggatc gtttgggccg taccagcgcc
     4681 aggtctgttc catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga
     4741 accttggagt tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg
     4801 gatgagatgg cgtataagtt ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt
     4861 ggttaaccgc gtcgcaagaa tttcactctc atggttgttg ctgcaaattg cagcaaaggg
     4921 taatcgatgg ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta
     4981 cataacatgt cgcccaaaat aacaacggtt caaccaagta aggtaaaacg ttatgcatgt
     5041 gcttaacatt ctctgggtgg tattcggcat tggcctgatg ctgatactga atttgaagtt
     5101 caaaatcaat tcaatggtgg ctttgttggt ggcggcgctg tccgtcggga tgctggcagg
     5161 catggatttg atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga
     5221 actggctatc atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc
     5281 ggctcaccag atagcacata cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc
     5341 ggtgattatc atcggcctta ttttcggtct ggcgatgttc tatgaagtgg cctttatcat
     5401 gttagcgccg ctggttattg ttattgccgc cgaagctaaa attccgttcc tgaaactggc
     5461 gatcccggca gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc
     5521 ggtggcgctg gtgaatgctt atggcgcgga tatggggatg gtttatatct atggcgtact
     5581 ggtgacgatc ccaagtgtaa tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct
     5641 tgagcgccca acgccatcat tcctgaaagc agatcaaccg gtagatatga ataatctgcc
     5701 ctctttcggc gtttcgattc tggtgccgct gatcccggcg atcattatga tctctaccac
     5761 catcgccaat atctggctgg taaaagatac ccctgcctgg gaagtggtta actttattgg
     5821 ttcctcgccg attgcgatgt ttattgcgat ggtggttgca ttcgtactct ttggcaccgc
     5881 gcgtggtcat gacatgcagt gggtgatgaa cgcttttgaa agcgcggtga agagtattgc
     5941 aatggtgatt ctgatcatcg gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg
     6001 cattggcgac accatcggca tgttgatgtc ccacggcaat atctcgccct acattatggc
     6061 atggctgatc actgtgctaa ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac
     6121 cgccgccggg attatcagtg ctgcaatcct tgatccagca accggtcagc tggttggcgt
     6181 gaatccggcg ctgctggtac tggcgacggc tgcgggttcc aacaccctca cccacattaa
     6241 tgatgcatct ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa
     6301 aacctggggt ctgctggagc tggtcaactc cgtggttggg ctgattattg tgctgattat
     6361 tagcatggta gcgtaaaaga acaccagagc ctgccaatgg caggctcaga ctgatgacta
     6421 aagtctaaaa atgtgcccgg acagtcccct cgcccctccg gggagagggt tagggtgagg
     6481 ggaacagacc agtaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct
     6541 ggcctacaaa gggctaacgt gcaggttttg taggtctgat agcgtagcgc ctcaggtacc
     6601 agcattagca atgcgctgta atttttttat tgataaacaa aagtcacgcc aataatcgat
     6661 tgcacattcc ctgcagtcac ctgccctccg gtacgtgcgt aatttgccgt taatcccaga
     6721 cttaccgccg aagttcctac tgctcctaac gataccgtgt tattcgctgg aataatcgta
     6781 ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg
     6841 aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt aataccccag gttttggctt
     6901 ttcgcacaat aaacggtaag aggaattggc actgaaccag ggtagtccgg cagagtaacg
     6961 gtgacatcac gagcagaaac atcacaaccg ccagtgggca ccaccacatc attattggcg
     7021 taaatattcc acacaaactg aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata
     7081 agcacggcaa ttaatgaacc agctttaata gccaccccgc ccgcactgct cacaggcgtc
     7141 aaataaagcg ccaccggcca cggcttatcc gttctcgaat tataaacaac ccgcggcgtt
     7201 tcgctggtgg tcggaaatgg atagctactg ccactatatt ttacggtccc ggaaaaatta
     7261 gataacacgc cgccgtaagc cgagcctcgt tgcagtgtga catagtctgt aatggtttcc
     7321 ggataatcgt tatggcaaaa gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca
     7381 ttcacgacag gcgcaaggtt tacataaaca ttagcgctgc caccgccaat agggatagcg
     7441 gtaccattgg cggttttaca ggcgaatgac caggcattta ccgaccagcc catcagcagt
     7501 acagcaaaca gggtaatagc tcgtttcatt acaattatct cttcgggttc agctgtaggt
     7561 ataggtgatg ctaatcactg cctgaatggt tccctgagtg gctccgccat ttaccgtcaa
     7621 tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa tcatccacct gaactgtttt
     7681 ggttgcgcca gtattcaatg tgttgccact gtcatcctgt agctctaact ggatgttttg
     7741 cgcggtcccc tggtttttat aatatccggt gctgtcggct gccccgctga agctggcagt
     7801 gaccctcgac gttcccaccg gacaattagt caactcaagc gcaacatcat gccaggccga
     7861 tgccgccccg gcagacataa gactgaaaga ataaagatcg ccgagatcta ccgtggcatt
     7921 ggtggtggaa accgtacacg gtttggcgac gaccttaccg ttcaccgtga tggtgacatc
     7981 ggctgcctgt atcgtcgcac ttgcgagcgc caacattgcc gccaatacat acccacgttt
     8041 gcaccatttc atgagcatct ccagttactg atattcaaga gtgaaggtag ccgtagcatt
     8101 gatatgcccc gcagtgacag gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt
     8161 atttggttta cccggcgtca gggtcgtcca cgaaatcgca gacgatggag cattaagggg
     8221 tatttgattt tgctgctcat tcagaagctg tattcccagt cccgcagccg ctgacaccgt
     8281 attttcaagt gcaagcaggt tggcattgtg gctatctgca acgccggtaa acccaacttt
     8341 tacggcagaa acggcattac cacagggtga cagcaaaata cgaaatggaa cgacaggagt
     8401 cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt
     8461 ggttgattca gcggccacac tacagccgtt atctctgaca tagccgcgga tagtaatcgt
     8521 gctatccgca gccaaaacgc gacttaccgc cagccacaaa aaagcgcaca gaagataaaa
     8581 aggtttgttt ctcatcacgc ccccttaacg acattcagct gatagctggg ttaataactg
     8641 ctgctgactc tctggtggca gttgataatt ggcgacacag tgagcatttt cctcttctcc
     8701 ccatttcacc tgaacttttc ccgctaaagg cattccgctg aggtaaacct gaccattatc
     8761 cgcaacaatg ccgctactct ggctactctc tgatgtcacc atcgccccaa acggcagcgg
     8821 cttattattg tgagttagcg tcatgagcag ttttatccca acgcgcgctt taaactctgc
     8881 tcgcacgatc gccccacgag tgggaacaac gttagcgacc gcgttatcta aatcgacgtt
     8941 atcagccaag gtattggtat ccagcgccac tctattttcc cgatattcag tggcataagg
     9001 cagcacggca taaccacgcc agtcggtacg cacccccgtc tggttttcga cttttgcatc
     9061 ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt aacggctgcc ccagcgttac
     9121 gccattggca tgagccaata ccccaccgct gactccgtaa tagagctgct taatatcatc
     9181 gctatggctg taaccgatat tggcattgcc gtaaccaccg cgataattca gcgtggcgta
     9241 gcctgtactt ccgctattac catcgcctcc cccggcatag ccggtttgca cgctataact
     9301 gaggttgttg tcttccagca acgtaccgta tacaccagcc agattggtca tccgaccgtt
     9361 gagatcgtgt gacatgctgt agctggcact ggcatgtcgc cactgagatt tactgtcaga
     9421 acgcagccag tggctgaaag gaatattgac gttaagcgct aacatctgat cccgtccttt
     9481 ttgccaggcg tttttcgtca ggctatagct gagcgtccag ttgatatctt cgaacgcagt
     9541 atttaatcca gcctggaatt gctcatcgac attactcgtt ccccaataag tttgatggct
     9601 accactcaaa tacagtgttg atgtgcgccc gagttgctga gtaacggtga gctgtaattt
     9661 cccgcgtttg ttataagcga ggttgtaata gtcggtgaat ttcggcttaa cctgaataac
     9721 tccatcctgt gtttcgatgt tgtagccatt cattcgactg tatgttgtat cagcgaaatt
     9781 aaaatatccg ctggtcgaat aacggtaacc cactaactga atattcgtgc ctgattcatt
     9841 gagcgatttg ttatagagaa aacgcaccga ttgtccgtca tgctgactgt catcgggaag
     9901 tgtggaatta gcctgcgtca tatccacaga cagagcgccc agtgcctcca tgtttttccc
     9961 gataccgaaa ttaaaagcac gataacgatc cgccagttgc gttccaccat atattgtcca
    10021 gccagccgga aggccgtgga gtaatgtact ctggaaaaag cggggttttt cctgctgcgc
    10081 atttccacta cggtattctc ctgccgtaat ggaataacga gtatgccctt cacgttgcaa
    10141 aagcgggact gacgaatagg gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat
    10201 cgttacctgc aagtcaccac tattacctgc ggcatagata tcgttgatgg taaaaggccc
    10261 cggtggcacc gtactattat aaatgccata tccattttgt ttaatagtga cctgtgcagt
    10321 accacgagca ataccgtgga tcaccggggc aaatcctctt tggctatcgg gtaacatatt
    10381 gtcatctgag gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt
    10441 ataaccatca cccaacgtca gccgggaacg taaaggaatt atgtctcgct caatccaggt
    10501 aatgatatgc tgccatttat ttttgctacc tgatgaactg tcgctactgt tataactcca
    10561 ggtggtattg tcgcgtaaac gccacgcacc aatatttaac ccactctgta ggtttaaata
    10621 tgcataatgg ctgttacccc caatccgatt
//
LOCUS       DBBBSB010000132        10570 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.132, whole genome shotgun sequence.
ACCESSION   DBBBSB010000132 DBBBSB010000000
VERSION     DBBBSB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.132"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..2152
                     /locus_tag="ACIUS0_003446"
     CDS             <1..2152
                     /locus_tag="ACIUS0_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGB0221055.1"
                     /translation="LEYLNCITNIINACGVYPQDLKDREIISTFHAEKVINDLLKNDY
                     KISLSPDTTYRELNKAAQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHF
                     THIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTL
                     ERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTT
                     FRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTF
                     NFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHP
                     EDIHLTAKDFEASKDNISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMR
                     KYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLV
                     TCQLNEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSER
                     RILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEI
                     KNYDGPTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRG
                     DSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPC
                     KVFTLRGGSLNINIPRRSALLLGKTGEPPNYLYL"
     gene            2488..3420
                     /gene="glaH"
                     /locus_tag="ACIUS0_003447"
     CDS             2488..3420
                     /gene="glaH"
                     /locus_tag="ACIUS0_003447"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGB0221056.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3440..4708
                     /gene="lhgO"
                     /locus_tag="ACIUS0_003448"
     CDS             3440..4708
                     /gene="lhgO"
                     /locus_tag="ACIUS0_003448"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGB0221057.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4731..6179
                     /gene="gabD"
                     /locus_tag="ACIUS0_003449"
                     /pseudo
     CDS             4731..6179
                     /gene="gabD"
                     /locus_tag="ACIUS0_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6187..7473
                     /gene="gabT"
                     /locus_tag="ACIUS0_003450"
                     /pseudo
     CDS             6187..7473
                     /gene="gabT"
                     /locus_tag="ACIUS0_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7711..9111
                     /gene="gabP"
                     /locus_tag="ACIUS0_003451"
     CDS             7711..9111
                     /gene="gabP"
                     /locus_tag="ACIUS0_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGB0221058.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9132..9794
                     /gene="csiR"
                     /locus_tag="ACIUS0_003452"
     CDS             9132..9794
                     /gene="csiR"
                     /locus_tag="ACIUS0_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGB0221059.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9795..10244)
                     /gene="kbp"
                     /locus_tag="ACIUS0_003453"
     CDS             complement(9795..10244)
                     /gene="kbp"
                     /locus_tag="ACIUS0_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGB0221060.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10328..10486)
                     /locus_tag="ACIUS0_003454"
     CDS             complement(10328..10486)
                     /locus_tag="ACIUS0_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGB0221061.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2663 a         2624 c         2732 g         2551 t
ORIGIN      
        1 attagaatat ctaaactgta taacaaatat tattaatgct tgtggagttt atcctcagga
       61 tttaaaagac agagaaatta tatcaacttt tcacgcagaa aaagtcatta atgatctgtt
      121 aaaaaacgat tataaaattt ccctttctcc agatacaact taccgagagc tgaataaagc
      181 tgcacagagt agcattacag cgccagacag gataggagaa gggaaaacat gggtttatca
      241 acgagataca atggttgaaa gaggtgataa cagtggtgtt catcagtatg gtccagctga
      301 acatttcact cacattatat ctgacaaacc gtccccaaaa gataagtatg ttgcatatgc
      361 tattaacatt cctgactatg agctagcagc cgatgtatat aatattaacg tgacgtcacc
      421 ttccggacag caagaaacat ttaaaatact aatcaatcca gaacatctac ggcaaacact
      481 tgagcgtaaa tctcttactg ctgttcagaa atcacaatgt gaaatcatca cccccaaaaa
      541 acctggcgaa gcgattcttc atgcttttaa tgccacctac cagcaaatca gggaaaatat
      601 gtctgagttt gcacgttccc attatgggta tatacaaatc cctccagtga caactttccg
      661 cgccgacgga ccagaaactc ccgaagaaga aaaaggttac tggtttcacg cttatcaacc
      721 cgaagatctt tgtaccatcc ataatccaat gggggatttg caggatttta tcgcattggt
      781 taaagatgct aaaaaatttg gtatcgatat cattcctgat tataccttta actttatggg
      841 aatcggggga agtggtaaaa atgacctgga ttatccctct gctgatatac gagcgaagat
      901 cagtaaagat atagaaagtg gtatccctgg ctattggcaa ggtcaggttt tgattccatt
      961 cattaaagat ccagtaacaa aagaacgtaa acaaatccat ccagaagata tacatctcac
     1021 tgcaaaagac ttcgaagcaa gtaaagataa catctctaag gatgaatgga aaaacctcca
     1081 tgcattaaaa gaaaagagtt taaatggaat gcctaaaaca acccccaaaa gtgaccaggt
     1141 tattatgttg caaaatcaat acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg
     1201 ttatgatgca gcaaaacact caaaacatga acaaatagaa agatcaataa ccccaccgct
     1261 taaaaattat aatgagcgat tacacaatac taacttattt aacccaaaat atcatgaaaa
     1321 aaccgttatg aattacatgg aatatctggt aacttgtcag ttgaatgaag aacaaatgtc
     1381 atcgctgctt tatgaaagag atgatttaag cgccattgat ttttcattgc tcatgaagac
     1441 gataaaagcc ttttcattcg ggggagatct cctaaccctt gcatcaaaac cgggttccac
     1501 aatctcaagc atcccatcag aaagacggat attgattaac attaaccacg attttcctaa
     1561 caatggcaat cttttcaatg actttctatt taaccatcaa caagatgaac aattagcaat
     1621 ggcatatatg gccgctctcc cgttcagcag gcctttagtt tactgggatg gccaggtatt
     1681 aaaatcaacg actgaaatta aaaattatga tgggcccacg cgtgtcggtg gtgaggcgtg
     1741 gcttaataaa ggttgctcta cctatcagca gctctacaat gaattccacg cattatatat
     1801 agataaagca ggaatatgga gcgcatttga gggtgtattt gcaactaaaa acgttctggc
     1861 ctttagtcgt ggggattctg tgaacattaa tcactctcct catgatggac tagttataat
     1921 aaataaagga aacgaagaag ttgaaggtac ctggcctaac aaattgcaac ctggaatata
     1981 taaaaacatg gggagtaata gcgttaacat tattattaat aatacccgaa aaattatccc
     2041 cccttgtaaa gtatttacgc ttagaggcgg atctctaaat atcaatattc ctaggcgtag
     2101 cgctcttctt ttagggaaaa caggagaacc gccgaactat ctctatttat aatttaatgt
     2161 tatatctgcc ccgataaaac ggggcagata atatgtttag tttactaacg gtcattttgt
     2221 agtgaagcca tttactgttt tttatcgacc agataatctg ttctctaatg ttaactcccc
     2281 ctaacctgtt gctttagtta ttcatttcct gtctcacttt gccttaatac cctacgttaa
     2341 atgttactaa tttgttgctt ttgatcacaa taagaaagca atatgtcgct tttgtgcgca
     2401 tttttcagaa atgtagatat ttttagatta tggctacgaa atgagcatcg ccatgtcacc
     2461 ctacatctca taagaggatc gcttctgatg aatgcactga ccgccgtaca aaataacgct
     2521 gtcgattcag gccaggacta tagcggattc actctcatcc cgtcggcgca atccccgcgt
     2581 ctgctggaac tcaccttcac cgaacagacg accaaacagt ttctcgaaca ggttgccgaa
     2641 tggcccgtgc aggcgctgga gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt
     2701 gacgatctgt gtgcgaatca gctgcaacca ttgctgttga agaccctgct aaaccgtgct
     2761 gaaggtgcgc tgttgatcaa tgcggtaggt atcgatgatg tggcgcaggc agatgagatg
     2821 gtgaagctgg caacggcggt agcgcatctg attggtcgct caaatttcga tgcgatgagc
     2881 ggtcagtatt acgcgcgttt cgtggtgaaa aatgtcgata actcagacag ctatctgcgt
     2941 cagccgcacc gcgtgatgga actgcacaac gacggcacgt atgtcgaaga gatcaccgat
     3001 tacgtgctga tgatgaaaat cgatgactgg gaacatctgg accactattt ccgccatcca
     3061 ctggcgcgtc gcccgatgcg ctttgccgcg ccgccgagca aaaacgtcag caaagatgtt
     3121 ttccatccag tgttcgacgt tgatcaacag ggtcgcccgg tgatgcgcta tatcgaccag
     3181 ttcgtccagc caaaagattt tgaagaaggc gtgtggttga gcgagctttc cgatgccatt
     3241 gaaaccagca aaggcattct ttctgtaccc gttcccgttg gcaaattcct gttgataaac
     3301 aacctgttct ggctgcacgg tcgcgaccgc tttactccgc acccggatct gcgccgtgaa
     3361 ctgatgcgtc agcgtggcta tttcgcttac gccactcacc actaccagac gcatcagtaa
     3421 gcgcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat
     3481 gtcgaccgcc atgcaactga ttgatgttta tccggatgcc cgcattgcgt tgctggaaaa
     3541 agagtccggc ccggcctgtc accagacggg ccacaacagc ggcgtgatcc atgccggggt
     3601 ctattacacg cccggcagcc tgaaggcgca gttttgcctg gcggggaacc gtgccaccaa
     3661 agccttttgc gatcaaaacg gcattcgcta tgacaactgc ggcaagatgc tggtcgccac
     3721 gtctcagctc gaaatggaac ggatgcgcgc cttatgggaa cgcacagcgg cgaacggtat
     3781 cgagcgcgag tggataaacg ccgtggaact gcgcgagcgc gaaccgaata tcaccgggct
     3841 tggtggcatt tttgtgccgt ccagcggcat tgtcagctac cgcgaagtga cggcggcaat
     3901 ggcaaaaatc ttccaggcca gaggcggcga gattatttat aacgccgaag tcagcgccct
     3961 cagtgagcat aaaaacggcg tggtgatacg cacccgtcag ggcggtgaat atgaagcatc
     4021 gacgctgatt agctgttccg ggctgatggc tgaccggctg gtgaaaatgc tcggcctcga
     4081 accgggcttt attatctgcc cgttccgtgg cgagtatttc cgccttgcgc cggagcataa
     4141 ccagattgtt aaccacctga tttaccccat tcccgacccc gcgatgccat ttttgggcgt
     4201 gcatctcacc cgcatgatcg acggcagcgt gaccgtcggg ccaaacgcgg tgctggcttt
     4261 caaacgcgaa ggctatcgca agcgcgactt ctcatttagt gacacgctgg agattttgag
     4321 ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa
     4381 aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc cccggctttc
     4441 gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg caggcagtat cgccggacgg
     4501 caagctgatt gacgattttc tgtttgtcac caccccacgc acgatccaca cctgcaatgc
     4561 gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca
     4621 aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga
     4681 tgccttacac gccgcattta atcaataacc tttgaaaaca ggacgtaaca atgaaactta
     4741 acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca
     4801 atggtgaagt catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga
     4861 aaatgggcgc agatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcct
     4921 ggcgtgcgct caccgccaaa gaacgcgcca ccattctgcg caactggttc aatttgatga
     4981 tgcagcatca ggacgattta gcgcgcctga tgaccctcga acagggtaaa ccgctggctg
     5041 aagcgaaagg cgaaatcagc tacgccgcct ccttcattga gtggtttgct gaagaaggca
     5101 aacgtattta tggcgacacc attcctggtc atcaggccga taagcgcctg attgttatca
     5161 agcagccgat tggcgtcacc gccgccatca cgccgtggaa ctttccggcg gcgatgatta
     5221 cccgtaaagc cggtccggcg ctggcggcag gctgcaccat ggtgctgaag cccgccagtc
     5281 agacgccgtt ctctgcgctg gcgctggcgg aactggcgat tcgcgcgggc attccggctg
     5341 gcgtgtttaa cgtggtcacc ggttcggcag gcgtggtcgg taatgaactg accagcaacc
     5401 cgctggtacg caaactgtcg tttaccggtt agaccgagat tggccgccag ttaatggaac
     5461 agtgcgcgaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg
     5521 tctttgacga tgccgatctc gataaagccg tggaaggcgc gctggcctcg aaattccgca
     5581 acgccgggca aacctgcgtc tgcgccaacc gtttatacgt gcaggacggc gtgtatgacc
     5641 gctttgccga aaaattacag caggcgggga gcaaactgca catcggcgac gggctggaga
     5701 aaggcgtcac catcgggccg ctgatcgatg aaaaagcggt agcaaaagtg gaagagcata
     5761 ttgccgatgc gctggagaaa ggtgcgcgtg tggtttgcgg cggtaaagcg cacgaacgcg
     5821 gcggcaactt cttccagcca accattctgg tggacgttcc ggccaacgcc aaagtgtcga
     5881 aagaagagac gttcggcccc ctcgccccac tgttccgttt taaagatgaa gccgatgtga
     5941 ttgcacaagc caatgacacc gagtttggtc ttgccgccta tttctacgcc cgtgatttaa
     6001 gccgcgtctt ccgcgtcggc gaggcgctgg agtacggcat cgtcggcatc aataccggca
     6061 ttatttccaa tgaagtggcc ccattcggcg gcatcaaagc ctcgggtctg ggtcgtgaag
     6121 gttcgaagta tggcatcgaa gattacttag aaatcaaata tatgtgcatc ggtctttaac
     6181 tggagaatgc gaaggaacag caataaagag ttaatgcagc gccgcagtca ggcgattccc
     6241 cgtggcgttg ggcaaattca cccgattttc gctgaccgcg cggaaaactg ccgggtgtgg
     6301 gacgtttaag gccgtgagta tcttgatttc gcgggcggga ttgcggtgct caataccggg
     6361 cacctgcatc cgaaggtggt ggccgcggtg gaagcgcagt tgaaaaaact gtcgcacacc
     6421 tgcttccagg tgctggctta cgagccgtat ctggagctgt gcgagattat gaatcagaag
     6481 gtgccgggcg atttcgccaa gaaaacgctg ctggttacga ccggttccga agcggtggaa
     6541 aacgcggtaa aaatcgcccg cgccgccacc aaacgtagcg gcaccatcgc ttttagcggc
     6601 gagtatcacg ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa tccgtactct
     6661 gcgggcatgg ggctgatgcc gggtcatgtt tatcgcgcgc tttatccttg cccgctgcac
     6721 ggcataagcg aggatgacgc tatcgccagc atccaccgga tcttcaaaaa tgatgccgcg
     6781 ccggaagata tcgccgccat catgattgag ccggttcagg gcgaaggcgg tttctacgcc
     6841 gcgtcgccag cctttatgca gcgtttacgc gctctgtgtg acgagcacgg gatcatgctg
     6901 attgccgatg aagtgcagag cggcgcgggg cgtaccggca cgctgtttgc gatggagcag
     6961 atgggcgtgg cgccggatct taccaccttt gcgaaatcga tcgcaggcgg tttcccgctg
     7021 gcgggcgtca ccgggcgcgc ggaagtgatg gacgctgttg caccgggcgg tctgggcggc
     7081 acctatgccg gtaacccgat tgcctgcgtg gctgcgctgg aagtgttgaa ggtgttcgag
     7141 caggaaaatc tgctgcaaaa agccaacgat ctggggcaga agctaaaaga cggattgttg
     7201 gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg ggctgggatc gatgattgcc
     7261 atcgagctgt ttgaagatgg cgatcacagc aaaccggacg caaaactcac cgccgagatc
     7321 gtggcacgcg cccgcgataa gggcctgatt cttctctcct gcggcccgta ttacaacgtg
     7381 ctgcgcatcc ttgtaccact caccattgaa gacgctcaga tccgtcaggg tctggagatt
     7441 atcggtcagt gttttgatga ggcgaagcag tagcaccgtc cccatgcagg aggcgacgct
     7501 gcgcgtcttg tccggcctac gagaatccaa gtcggataag gcgtttacgc cgcatccgac
     7561 aatctgtacg tgaacaggaa taaatctatg tcggccggat aaggcggagc cgctctccgg
     7621 caaaaagaat caataacaat taaacgcgtg acccgacgcg ggaaatgtcg gggcgctctc
     7681 ccaagtgaca cactttcgag aggattcagg atggggcaat catcgcaacc acacgagtta
     7741 ggcggcgggc tgaaatcacg ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca
     7801 agtctgtttg tcggttccag cgtcgccatc gccgaagcgg acccggcggt attactggcc
     7861 tatctgttcg ccggattact ggtggttatg attatgcgga tgctggcgga aatggcagtt
     7921 gccacgcccg ataccggttc gttttccacc tatgccgata aagccattgg ccgctgggcg
     7981 ggctatacca tcggctggtt gtactggtgg ttttgggtac tggttatccc actggaagcc
     8041 aacatcgccg ccatgatcct gcactcgtgg gttccaggca ttcccatctg gttattttcc
     8101 ctcgtcatta ccctcgcctt aactggcagt aatttattaa gcgttaaaaa ctacggcgaa
     8161 tttgagttct ggctggcgct gtgcaaagtc atcgctatcc tggcctttat tttccttggt
     8221 gcagtcgcaa ttagcggttt ttacccttat gccgaagtga gcgggatctc aagattgtgg
     8281 gatagcggcg gctttatgcc caacggtttc ggtgcggtat taagcgcgat gttgatcacc
     8341 atgttctcgt ttatgggcgc agaaattgtc accattgccg ccgcggaatc cgacatgccg
     8401 gaaaaacata ttgtccgcgc caccaactcg gttatctggc gtatttctat cttctatttg
     8461 tgctctattt ttgtcgtcgt ggcattaata ccgtggaata tgccggggct gaaagccgtt
     8521 ggatcttatc gctcggttct ggaattgctc aatattcccc atgcgaaatt aatcatggac
     8581 tgtgtgatat tactttccgt aaccagttgc ctgaactcgg ccctgtatac cgcctcaagg
     8641 atgctctact ccttaagccg tcgcggtgat gctcccgcgg taatgggtaa aatcaaccgc
     8701 agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg cagcattctt aacggtggtg
     8761 gtgaactatt acgcgcctgc gaaggtattt aaatttctga tcgacagctc cggcgctatc
     8821 gccctgctgg tttatttagt catcgccgtt tcacagttgc ggatgcgcaa aattctgcga
     8881 gcagaaggaa gcgaaattcg cttgcgaatg tggcttgatc cctggctcac ctggctggtc
     8941 atcggcttta ttacctttgt gttggtagtg atgctattcc gtccggcgca acagttagaa
     9001 gtgatctcca ccggcttatt agcgataggg attatctgta ccgtgccaat tatggctcgc
     9061 tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata atacgcgctg ataatgatca
     9121 ggagtcacac catgaccatt acgtctctgg atggctatcg ctggctgaag aacgatatta
     9181 ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag tttactgaca tcgcgttatg
     9241 cacttggcgt tgggccgttg caggaagcac tttctcaact ggtggcggaa cggctggtca
     9301 cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag ctgctcgata
     9361 ttttcgacgc ccgcgccaat atggaagcga tgctggtcag tctggcgatt gcccgcggtg
     9421 gcgatgaatg ggaggcagac gttctcgcaa aagcgcatct gctcagtaag ctggaagcct
     9481 gtgacgccag cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg tttcatacag
     9541 caattgtggc gggctgtggt tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc
     9601 tggcggcgcg ttatcgattt atctggctgc ggcgaacggt gctttcggtg gaaatgctgg
     9661 aggataaaca cgatcagcac cagaccctga ctgcggcggt actgccgcga gatacagcgc
     9721 gcgccagtga gttaatgcgc cagcatttac tgacgccaat tcccattatc cagcaggcga
     9781 tggctggcaa ttaactactc ttccggaata cgcaacactt gccccggata aattttatcc
     9841 gggcttttta gcatcggttt attcgcttcg aagattttat tgtacagatt agcgttaccg
     9901 tagacctgtt tggaaatggc actcagagtg tcgccagact taacggtata aaactggctg
     9961 gcagtggctg gtgtcgccgt tttcacctga tcatcaacac tggcaatacc ggaaatattc
    10021 cccaccgcaa caaggatttt ctccttcgcc tcctgactca ggccgtcacc agtgaccgtc
    10081 gctttgccgt cggcaatttg aatgttcact ttatcggcat caggtatacc ggttttgctc
    10141 agatgctcct gcaccttctt cgcctgatcg tctttatcgt gctgacctgt aaccgcatcc
    10201 cagagttttt ctccggcatc tttaacaaaa ttgaacagac ccatagctac ctcattcgtt
    10261 aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc gttaacgccg cgggggtgag
    10321 cacaggttta atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg atatacccaa
    10381 gcagtgtcag cagaatatta ataatgaacg cccaaccgaa ccctttaccg agcagcacgc
    10441 cgatcggcgg cagaatgatg gtgatgacga ttctccagaa acccatatgt actccctata
    10501 agaaaattac tcattgttta aaaagagatt ttatctctta agcgtagtaa ttttagcgga
    10561 gcctgccagt
//
LOCUS       DBBBSB010000133        10446 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.133, whole genome shotgun sequence.
ACCESSION   DBBBSB010000133 DBBBSB010000000
VERSION     DBBBSB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.133"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACIUS0_003455"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACIUS0_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGB0221062.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACIUS0_003456"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACIUS0_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGB0221063.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACIUS0_003457"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACIUS0_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGB0221064.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACIUS0_003458"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACIUS0_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGB0221065.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACIUS0_003459"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACIUS0_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGB0221066.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACIUS0_003460"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACIUS0_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGB0221067.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACIUS0_003461"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACIUS0_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGB0221068.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACIUS0_003462"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACIUS0_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACIUS0_003463"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACIUS0_003463"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGB0221069.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACIUS0_003464"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACIUS0_003464"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGB0221070.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACIUS0_003465"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACIUS0_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGB0221071.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2561 a         2658 c         2613 g         2614 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacggtaa aaaaagcaca ttatgcaaaa
    10441 ttcatt
//
LOCUS       DBBBSB010000134        10372 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.134, whole genome shotgun sequence.
ACCESSION   DBBBSB010000134 DBBBSB010000000
VERSION     DBBBSB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.134"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..338)
                     /locus_tag="ACIUS0_003466"
     CDS             complement(<1..338)
                     /locus_tag="ACIUS0_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGB0221072.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACIUS0_003467"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACIUS0_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACIUS0_003468"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACIUS0_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACIUS0_003469"
     CDS             complement(1445..2008)
                     /locus_tag="ACIUS0_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGB0221073.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="ACIUS0_003470"
     CDS             complement(3383..5218)
                     /locus_tag="ACIUS0_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221074.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="ACIUS0_003471"
     CDS             5319..5606
                     /locus_tag="ACIUS0_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0221075.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="ACIUS0_003472"
     CDS             5578..7107
                     /locus_tag="ACIUS0_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HGB0221076.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="ACIUS0_003473"
     CDS             complement(7277..8494)
                     /locus_tag="ACIUS0_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221077.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACIUS0_003474"
     CDS             complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACIUS0_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGB0221078.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="ACIUS0_003475"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="ACIUS0_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3232 a         2371 c         1948 g         2821 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttactttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gc
//
LOCUS       DBBBSB010000135        10215 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.135, whole genome shotgun sequence.
ACCESSION   DBBBSB010000135 DBBBSB010000000
VERSION     DBBBSB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.135"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            84..629
                     /locus_tag="ACIUS0_003476"
     CDS             84..629
                     /locus_tag="ACIUS0_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HGB0221079.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGI
                     SYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFC
                     ISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQL
                     SRLDLASPVLEPEVIWLGRRR"
     gene            766..1338
                     /locus_tag="ACIUS0_003477"
     CDS             766..1338
                     /locus_tag="ACIUS0_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGB0221080.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            1375..2766
                     /locus_tag="ACIUS0_003478"
     CDS             1375..2766
                     /locus_tag="ACIUS0_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HGB0221081.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            2799..3227
                     /locus_tag="ACIUS0_003479"
                     /pseudo
     CDS             2799..3227
                     /locus_tag="ACIUS0_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3546..4202)
                     /locus_tag="ACIUS0_003480"
     CDS             complement(3546..4202)
                     /locus_tag="ACIUS0_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HGB0221082.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            4597..5612
                     /locus_tag="ACIUS0_003481"
                     /pseudo
     CDS             4597..5612
                     /locus_tag="ACIUS0_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5688..8438)
                     /locus_tag="ACIUS0_003482"
                     /pseudo
     CDS             complement(5688..8438)
                     /locus_tag="ACIUS0_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            8452..8796
                     /locus_tag="ACIUS0_003483"
                     /pseudo
     CDS             8452..8796
                     /locus_tag="ACIUS0_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
     gene            complement(8843..9718)
                     /locus_tag="ACIUS0_003484"
     CDS             complement(8843..9718)
                     /locus_tag="ACIUS0_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /protein_id="HGB0221083.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(9907..>10215)
                     /locus_tag="ACIUS0_003485"
     CDS             complement(9907..>10215)
                     /locus_tag="ACIUS0_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HGB0221084.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         2512 a         2579 c         2643 g         2481 t
ORIGIN      
        1 aaataaccac catcatttca taacaatacc tgctgactat gaagatctac gtcatcaaaa
       61 tagcagttca tggagtcagt ccgatggtct ggcgtcggct gagaattgct gccgacacgt
      121 cactggccgc gcttcacttc attttccaga tagtgcaggg ctggggggac gaccatctcc
      181 atcaatttca catttatggc aaagattacg gtatctccta cgaaggaggc atcggttttg
      241 tcgataatcc gttccgggtc gtgattgatg attttgcttt tgatgctggc gatcgcttta
      301 cctatgaata caatttcttt gagcactggc ttcatgacat tcgtgttgag gccatttatg
      361 aaaactctac gctgaaagcg ccgttttgta taagcggcca tggtatgccc ggagccacag
      421 ctgcggatga gttcgataaa accctggcgt ttcttgaagc tatcgttaat gcggatgatg
      481 aaacaacggt cggcgagatc cgcccttttg ctgacgacct ggatgctgtc aggttcaacc
      541 gccacaaaat caaccggcaa ctgagcaggc tcgaccttgc atctccggta ctggagcctg
      601 aagttatctg gctgggccgt cgtcgctgac tgatgtctat aaaggtatac gttttcagca
      661 tgcctctcag atccccgaaa aaatgtctat aaacctccct aaatcagtgt ttatggatta
      721 ttctttaaat ctctatgatg tttaaagacc ttccggaggt tttgcgtgca atatgcctac
      781 atcagggtaa gctcggccga tcagaatacg gcccgtcagg aagaggcatt gtcaaaggcg
      841 ggttttcagc ctgataaaat ttgtgttgag catgccagcg cgaaggacac aaaccgcccg
      901 gggttacagg aactcctggg gcaattacgc cctggtgata cgcttctggt tcattccatc
      961 gatcgccttt gccggaatat gtcggatatg tgcgctgtca ccacccggct tcgtgaccag
     1021 ggcgttaccc ttatttttct gaaagagcag ctgacgttca gcgccggcac aaacaatccg
     1081 atgcaggaac tgcagctgca catgatgtcg gcattcagcc agtttgaacg agccttactg
     1141 aaggaaaggc aggccgacgg catcgccgcg aaaaaagcac ggggtgaaaa aacagggcgc
     1201 cccggcgccg atatcaaaaa gatccaggaa attgacgcac tcagaagcag aggggtcagg
     1261 ctcaggatcg cctgcgacca tgcgggcctt ggcgtttcca catattataa gcttcgtcac
     1321 cagatgaaca accagtagcc gggagatagt catccaccac agcaggaggc aatgatgcaa
     1381 ctgacgctcc agatagtcat caccgatgaa tcaggctcca gtcgaacgga ggaactgatg
     1441 acaatccaga aatcagggga gacccggaac gacatcggat tatcggtgtc agaatccaag
     1501 ctgttgctga atacggtcca gcagtcggtg gtccagctgc aggcagacga atatactcaa
     1561 caccatatcc ggtgccctca ctgccttgct gcgcgcagaa tcaaaggcaa acagaaaata
     1621 cggtaccgga cgctgtttgg cgttattccg gtatccgggc ttcgggtgta ccggtgccgt
     1681 tgtgaagaga gtgatacaaa gacagtcagt ttgctcagcg actgggccgg cgattattct
     1741 cacccggcac tgaaatatat tgaaacccgc tgggcctcga tgatctccta tgaaatgacg
     1801 actcgtctgc tgaaagatat tctgccggtg ggccacagcc tgaatgcctc aacggtgagg
     1861 aatcatttat gccaggtggc gcagcgtctt gacgctgagg ctgaagctca ttcaggtttt
     1921 ctttccggct gcccccgcga ctgggggaac ctgcccaggc caggaaaacc gcttgtggtg
     1981 ggtatagatg gtggttatgt tcgcgatcgg gatgacaaaa agcgtaattt tgaaattatt
     2041 gccgggaagt ctttttccgt tggtgcgcct gctgacaccc gccgcttcgg ttttgttcag
     2101 aaggatgact gtcatccgga acgcaggctc atgacccacc tttcagcgca gggaatgcag
     2161 gccaaccagc agatattttt tctgtccgac ggcgcggata atctcaggga cctccagttc
     2221 ggtatgtacc ctgagtcaac gcatgtgctg gactggtttc atatcaccat gaggctgaag
     2281 gtgctcatgc aatatgcccg gggactgctg gtatcagatc cagaggcggg gagtaaagtt
     2341 ctggcactgc tggaaagcat taaacgatat ctctggcatg gcaatgttgt cgctgcgctg
     2401 gaacatattg ataattgcgt tatgtattgc gatgaccctg aactcagcta tcctagcctg
     2461 aaatccctgc aaaaacatct ggatgaaatg tatacctaca tccggaataa taagatgatg
     2521 atcccaaact acggcgaaat gcgtcggtac ggagagccgg tatcaaccgc gtttgtggaa
     2581 tccacgatca atgaagtgat cgccagacga atggccaaaa agcagcagat gcagtggagc
     2641 agaaaagggg ctcattattt gttacagacc cggaccgccg ttttgaataa cgaactgcag
     2701 gataaatttg tctgctggta tccgggtttt caaagtgacg ggaagggacc agcgatggca
     2761 gcatagctca cgctgcccca atatttttta tgctctcggt cgtatggaag ctggaccgcc
     2821 tcggtcgtga tcttcgtcat ctcattaata cagtgcacga cctgaccgcg agggggaccg
     2881 gtctgaaagt gctgaccggt cacggggcga ctatcgacac aacaacggcc gctggcaagc
     2941 ttgtcttcgg tatctttgcc gcactggcgg agtttgagcg cgaactgatt gctgaaagaa
     3001 cgactgcagg tctggcatca gcaagagccc gtgggcggaa cggcggccga ccttataaaa
     3061 tgacgccagt caaactgcga ctggcaatgg catcaatggg acaatcagaa acaaaggtca
     3121 gtacgctatg ccaggaactt gggataaccc ggcaaacttt gtacaggcat atttcccctg
     3181 ttggtcaact gcgggcggac gggatcaagc tgctcaaccg tggataattt gaacggacgg
     3241 aaaaatctgg ggttccggcg tagtacccct atcacctcca catttttgca acagtgcctt
     3301 ataaatggca gccttatgtt tacttgccaa gatgggctac catatgatgt tttatactga
     3361 gcaatattaa tattatcttt aatggaaaaa ttaagcccac taagtgaccc actgctatat
     3421 ggctctattg tgaagacgcc tgagggtaaa aatttataca tggttgtccc tatgtctatt
     3481 attgcaaggt tgacaatatt attcgtttta taaatttgag cgtttaaaat cacacatcaa
     3541 agcaattagt caggataaac aacaataccc agtgcttcga gaatcagttc ttgctgccag
     3601 gctgcaattt tgatacctga tgtatcgact ttgcggggat ctaaaccgtc gagttcggcg
     3661 tggcataaat tggcaccctg taggctaaat tgcccccaga catcttcgga aaaaacacct
     3721 cgacttaagt ctgactcttt cagtgatgca cccgctaggt tcgttcctat ccagcgattt
     3781 tcaaacaact cacatttttc taaacaaacc ctctccatat tggcatagga aagattacat
     3841 ccagaaataa atgctgagca aaagtacata cgattactca cttgatgggc aaagtttgtt
     3901 cgggaaaagt tggcaccttt taaatcacac gcacggaact ctataccgta gcaattggca
     3961 ttactgaagt ttgccatcgc aagttggcat tgttggaaac ttgcatcacg aagatctgcg
     4021 acatcaaagt ggcagccttc gatatcaccc tgttcaatga acttgcagtt gacgaatgtc
     4081 gtatcacgca agttagcacg tcgaaagtcg ctgcgaatga atgtgcaagc ggtgaaggtg
     4141 agatcactta agtctttatg tgaaaagttg tggtgccgat atgtatgatt gtaggtttcc
     4201 atgagtactc cttgttgaaa ggagggctag actaccatta tacatatgcc aagacattaa
     4261 aataaaagcc tgttacatca acacattaca acccctcaat ttacctgttt taagtggtca
     4321 aattggcgcg tagagcgcct atcgctcttg cattggcacg cggccctgtt gaaaaaagtt
     4381 attcgcttta gatagggtta ggagagccgt gcttcgccgg taaaaaatcg gactatccga
     4441 cgcagggcgg actcatcggc ggagaagcaa actttgacta tatctcccta cacttggaag
     4501 agggctagac tggccccata gatcaccaga cacttcgact ctcttaaaat aagaggtagt
     4561 cttggaacta tgtttaatac cagtcagaac gataagatgg ttgaagtgtt atcaggacct
     4621 gagcggcgtc ggcgtcgtac accgcaggaa aaaattgcca ttattcagca gactatggag
     4681 cccggtatga ccgtgtctca tgtcgcgcgc ctgcatggca tcaatgcaaa ccagatattc
     4741 aaatggcgca agcaatatta agacggttca ctgacggctg ttgcatcggg cgaggaagtg
     4801 gtgccggcct ccgaactcgc tgccgccaat aagcaaattc gcgaactcca gcgccttctg
     4861 ggcaaaaaga cgatggaagc cgagatactc aaagaggccg tggagttcgg ccgggcaaaa
     4921 aaatggattg cgcatgcgcc ctcgttgcct gggaacgacg aataagcgac gtttgcagaa
     4981 gtcttggcgt gtcgcgtgcg cagttgagca ttcgggttca ccggccatct gactgacagg
     5041 atcgcagacg gcagccccgt tctgacgata ccaccgtatt gtcccggata aacatggcgg
     5101 tggctgacct gccgacgtat ggttatcgcc gtgtgtgggc gctgctgcga cgggagtcgg
     5161 agcgggacgg cctgcctgtg gttaatgcaa agcgggtgta tcgcattatg agaacgcata
     5221 atctgcttct tgaacgtaaa cccgctgatc cttgccgtaa gcgggcccat aaggggcgcg
     5281 tagcagtagc ggaaagtaac cggcgctggt gctcagatgg ctttgagttc cgctgtgata
     5341 atggcgaaaa gctgcgggtg accttcgcgc aggactgctg cgacagggag atcattgact
     5401 gggcagcgag caccggaggc tatgacaaag aaacggtgca ggatgtcatg ctgggtgcgg
     5461 tggaaaaacg cttcggaagg cagttgccaa cagtgccggc gttcgcccgg ttgctgggac
     5521 tggagccttg tacgacagca gtgcgaagcc cggaaagcaa tggcatagca gaaagcttcg
     5581 tgatgacgat aaaacgggac tacatcagca tcaggatttt aggggtctga cgctcagtgg
     5641 aacgaaaact cacgttaagc aacgttttct gcctctgacg cctcttttaa tggtctcaga
     5701 tgtcctttgg tcaccagttc tgccagcgtg aaggaataat ggccgagcat attgatatgt
     5761 ccgtggcaaa gcggggagag gcgtgcgata tcttcatcat tcagtgtttc accctgcgcc
     5821 cggagatgat ccagggctgc ctgcatataa atagtgttcc ataacacgac ggcgttagtg
     5881 accagcccca gtgtgcccag ttgatcttcc tgaccgtcgg tatatcgttt tcttatctca
     5941 cctttttgac cgtgacagat ggctctggca acggcatggc gactttctcc ccgattaagc
     6001 tgggtcagaa tgcgccggcg gtaatcttca tcatcaatat aattaagcag atacagcgtt
     6061 ttgttgatgc gccccacttc aatgattgcc tgagtcagtc cggaaggacg ttcacttttc
     6121 agcaatgaac ggaccagcac tgaaacctgt actttgccca gcttcaggga gccagcggtc
     6181 cggatcattt cgtcccactg aaggactatt tttcggggat ctgattgccc tctggcaata
     6241 tcattcagca cgccatagtc ggcatcatgg tccattcgcc agaaaaccga agcaccggca
     6301 tcagccaggc gtggagaaaa ctggtatccc agcagccaga aaaggccaaa gacaagttcg
     6361 ctggcacctg ctgtatcggt cataatttcg gttggattca gcccggtctc ctgttccaga
     6421 agaccttcca gcacaaagat agagtccctc agcgtccccg gtataacgat gccatgaaag
     6481 ccggaatact gatcggacac aaagttgtac caggtgatcc ctctgttatt accaaagtat
     6541 ttgcggttcg gtccggcatt gattgttctg actggcgtaa caaagcgcat tccatctgca
     6601 gatgccactt ctcctccacc ccatatctgt gccagtggca gcgttgcctg aaaatcaacc
     6661 agtctggcat tagcgctggt gatagtttca gcccgcagat agttcgcttt tgtccagttc
     6721 agccggtgtc gggtcagtgc aggaacattt gatctgatca gtggttccag accgatattg
     6781 caggcttcag ccatcagcac ggcgctgatg ctgacgggca gatcatcaac tctggcactg
     6841 gcttcactag catggaaaaa ctcatcagca aatccggtat gggcgttaat ttcgagcagc
     6901 aactccgtta aatccaccgg agggagtaga tcactgatca ttttgctcag tcgtttcaga
     6961 ctgtccggct catcaagact ggcgagggga gaaattgtca accggggctt cgggccagaa
     7021 acatcgagtt cgacagcctc attttcgcaa agacgtgcag caacctgtct gtaacgacta
     7081 tcaagctgat gacccagaga ttttattgct tcctgcgggt ctgtcgggtg ccccaaagaa
     7141 cgataaacct taatccggtt tgcctgccag tcagcaccct gtagtaatct tgcacgagga
     7201 tctccccacc ggttactgcc ggtaacgtag acatccctcc gcctcagact atcctgcagt
     7261 ttactgagaa agcagagcgt gtatcccctg cgggtgatat gtttttcctt gttaatcacc
     7321 agccgtttcc atgaccgact gataatttcc gttggtgcgt cgtcaaaaaa ctgccgccgt
     7381 gagctgaact cccggctgag gtagtcacag gcattcagag tggtaacccc ggcaggtgcg
     7441 gatgaaaatt taacggtatt cagcagatgg ggcaggaaac gacgaacgcg cccgtactgc
     7501 tccaccattt cttcatgaaa attatcgtct gagggccggg caatttcacg gacaagcgtg
     7561 atgatttcag ccagcttttg ccttgggatg tagctgaaca cctcagcacg aatcgattcg
     7621 tccggtgttt cttctttcag caggtacgaa catgcgctgg cgagcgccaa tgcagattta
     7681 tccagatcct tcagcgagcg gagccgtttt ttctgcccaa tctttctggc gtcacggatg
     7741 ataacggcca gcatggcgtc cagaacgtcc aatgcatcat ccagcgccag cgtttcccat
     7801 gcaaggacaa aggcaaccag aaccgccatc cttttctgcg gtgacatcct ggcaatattg
     7861 aacaccgaag tcataccagc ataacgtgcg agatttttca ggcgcacagc cgggagtgta
     7921 ctcaggtttt cagcatgcag gccaaaatcg ttcagagttt tccagcgttc aattgcttca
     7981 ttaaacgccg gaccactgat ggtcacaggg ccctttttca gtgattccag taaagacagg
     8041 cggctgcaat cagttggccc cagcagcatc tccagctgtg aacgctgttc ggctgacggt
     8101 atcagtgcca gtttgttcca caggcgcaac gtcgcctttt cccttacctc tgaaatcaac
     8161 cgggtcagcg tagtggctcc ggggagaata atacgatgtt gcataagcca ccctgtcgcc
     8221 agatcgaaaa gcaggccagg acgttcgttg cttatccagc tccgggtata taaaagacgg
     8281 gtaaggcgaa atgtccaggg ccaggcaaat tcacgatact gatagtgctg acgtatcagc
     8341 gctgcatgct cacggcgggt attttccctc tgaccgtatt ctgcaagaac ggtgatatca
     8401 cgaatcccga gctgtctggc ggtaaaatgc cggacgccgg aaggaatatg acctgggacc
     8461 tacgtgcgcc cgcaccgaca ccctcacacc ttcgagctac tgttgccatt aaggggtcgt
     8521 ttcgtggtgc tgaattttga cgatcggggt accgtcaccc atcgggcgat attgggggaa
     8581 acctgtacgg tgctggagat ggccgcagga acctggcatg ccgtgctgtc gctggatacc
     8641 ggtggcataa tttttgaagt aaaacacggt ggctatcaac ccgtggctgc cgatgactat
     8701 gcgcactggg ctccagcgga aggagaacca ggaaccacgg agcttatggc ctggtatgcg
     8761 caagcgcagg tgggcgacag cacttttgcc gtctaaggcg ataaacaaaa acggaatgag
     8821 tttccccatt ccgtttccgc tattacaaac cgtcggtgac gattttagcc gccgacgcta
     8881 atacatcgcg acggctttct gccttaggtt gaggctgggt gaagtaagtg accagaatca
     8941 gcggcgcacg atcttttggc cagatcaccg cgatatcgtt ggtggtgcca tagccaccgc
     9001 tgccggtttt atcccccaca acccaggaag caggcagtcc agcctgaatg ctcgctgcac
     9061 cggtggtatt gcctttcatc catgtcacca gctgcgcccg ttggctgtcg cccaatgctt
     9121 tacccagcgt cagattccgc agagtttgcg ccattgcccg aggtgaagtg gtatcacgcg
     9181 gatcgcccgg aatggcggtg tttaacgtcg gctcggtacg gtcgagacgg aacgtttcgt
     9241 ctcccagctg tcgggcgaac gcggtgacgc tagccgggcc gccaacgtga gcaatcagct
     9301 tattcatcgc cacgttatcg ctgtactgta gcgcggccgc gctaagctca gccagtgaca
     9361 tcgtcccatt gacgtgcttt tccgcaatcg gattatagtt aacaaggtca gattttttga
     9421 tctcaactcg ctgatttaac agattcggtt cgctttcact tttcttcagc accgcggccg
     9481 cggccatcac tttactggtg ctgcacatcg caaagcgctc atcagcacga taaagtattt
     9541 gcgaattatc tgctgtgtta atcaatgcca cacccagtct gcctcccgac tgccgctcta
     9601 attcggcaag tttttgctgt acgtccgccg tttgcgcata cagcggcaca cttcctaaca
     9661 acagcgtgac ggttgccgtc gccatcagcg tgaactggcg cagtgatttt ttaaccatgg
     9721 gattccttat tctggaagat acgaaataac aacaacatga atagtcccta aattccacgt
     9781 gtgtttttta ttagcttcaa aaatcactat ttcacgaaga atttagactg cttctcacac
     9841 attgtaacat tattggcact gttgcaaagt tagcgatgag gcagcctttt gtcttattca
     9901 aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg gatcaccata
     9961 ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac ctttgatggt
    10021 ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ttgattatcc gtttcagttt
    10081 gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt caacgtcaga
    10141 cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg gcgctttatc
    10201 cgtgttgatg aatcg
//
LOCUS       DBBBSB010000136        10149 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.136, whole genome shotgun sequence.
ACCESSION   DBBBSB010000136 DBBBSB010000000
VERSION     DBBBSB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.136"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            386..1252
                     /gene="folD"
                     /locus_tag="ACIUS0_003486"
     CDS             386..1252
                     /gene="folD"
                     /locus_tag="ACIUS0_003486"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGB0221085.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1254..1466
                     /gene="ybcJ"
                     /locus_tag="ACIUS0_003487"
     CDS             1254..1466
                     /gene="ybcJ"
                     /locus_tag="ACIUS0_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGB0221086.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1574..2083
                     /locus_tag="ACIUS0_003488"
     CDS             1574..2083
                     /locus_tag="ACIUS0_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGB0221087.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2119..3504)
                     /gene="cysS"
                     /locus_tag="ACIUS0_003489"
     CDS             complement(2119..3504)
                     /gene="cysS"
                     /locus_tag="ACIUS0_003489"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGB0221088.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3678..4172
                     /gene="ppiB"
                     /locus_tag="ACIUS0_003490"
     CDS             3678..4172
                     /gene="ppiB"
                     /locus_tag="ACIUS0_003490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGB0221089.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            4175..4897
                     /gene="lpxH"
                     /locus_tag="ACIUS0_003491"
     CDS             4175..4897
                     /gene="lpxH"
                     /locus_tag="ACIUS0_003491"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGB0221090.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5015..5524
                     /gene="purE"
                     /locus_tag="ACIUS0_003492"
     CDS             5015..5524
                     /gene="purE"
                     /locus_tag="ACIUS0_003492"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGB0221091.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            5521..6588
                     /gene="purK"
                     /locus_tag="ACIUS0_003493"
     CDS             5521..6588
                     /gene="purK"
                     /locus_tag="ACIUS0_003493"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGB0221092.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6725..7618)
                     /locus_tag="ACIUS0_003494"
     CDS             complement(6725..7618)
                     /locus_tag="ACIUS0_003494"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGB0221093.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7615..8262)
                     /locus_tag="ACIUS0_003495"
                     /pseudo
     CDS             complement(7615..8262)
                     /locus_tag="ACIUS0_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            8265..9497
                     /gene="allC"
                     /locus_tag="ACIUS0_003496"
     CDS             8265..9497
                     /gene="allC"
                     /locus_tag="ACIUS0_003496"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGB0221094.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            9508..>10149
                     /gene="allE"
                     /locus_tag="ACIUS0_003497"
     CDS             9508..>10149
                     /gene="allE"
                     /locus_tag="ACIUS0_003497"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGB0221095.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2361 a         2722 c         2685 g         2381 t
ORIGIN      
        1 ctaagaatgt cttaattgaa aatatgcact ctattctaaa aaatagagag ccccgttaga
       61 tgaatacttc cgcgcaaaat atattcacca caaatataga cctgaagcgg taaattacca
      121 ggctgaaaat tctttttata ttgtcaggta tttcttaaat tatcttaatc cttaaacaag
      181 gaaataaatc agttccagat ttacaacgcc atcatgtacg aaaaatgaag ctttcaatct
      241 cagcgacctc agcgacggtg cgcctaacct tcgcaagagg tcgcttcacg cgataaatct
      301 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata gacgcatccc cttcgaccta
      361 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      421 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggct cgtattgcag ccggactgcg
      481 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      541 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      601 aaccaccagc gaagcggagc tgctggagct tatcgatgcg ctgaatgccg acaacaccat
      661 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      721 acgtattcat ccggacaaag acgtggacgg tttccatcct tacaacgtcg gtcgtctgtg
      781 ccagcgcgcg ccgcgtctgc gtccctgcac cccgcgcggt atcgtcacgc tgcttgagcg
      841 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      901 tcgcccaatg agcatggaac tgttgctcgc aggttgcacc accaccgtga ctcaccgctt
      961 cactaaaaat ctgcgtcatc acgtagaaaa tgccgatctg ttgatcgttg ccgttggcaa
     1021 gccaggcttt attcccggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
     1081 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
     1141 cgcctcatac attacgcctg ttcccggcgg cgttggcccg atgacggttg ccacgctgat
     1201 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1261 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1321 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagtcgacg
     1381 gtgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1441 gacacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1501 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1561 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttccccttt gcattggttt
     1621 agggttgggt tcgaaaggtc tgttatttgc cggaatcatc ctggcgatgc tgcccgatgc
     1681 cgacgtattg tcgtttaaat ttggcgttgc ttacggcaat gtttttggtc atcgcggttt
     1741 tacccattcg ctggtgtttg cgtttgttgt cccgctctta tgtgtgctga ttggacgtcg
     1801 atggttcagg gctgggctga ttcgctgctg gctgttttta accgtctcgt tgctatcgca
     1861 cagcttactg gattcggtaa ccactggcgg taagggcgtt ggctggctgt ggccgtggtc
     1921 agatgaacgc tttttcgctc cctggcaggt gattaaagtc gcgccgtttg cattgtctcg
     1981 ttacaccaca gcgtacgggc atcaggtgat tatttccgaa ttgatgtggg tgtggttgcc
     2041 ggggatgcta ctgatgggaa tgttgtggtg gcgcagacga taaccggatg cgaaaactcg
     2101 catccggcaa ttgcgcaatt acttacgacg ccaggtggtc ccttgcgggc catcttccag
     2161 cacgatcccc atctcgttaa gacggtcacg tgccgcatct gccgccgccc agtctttcgc
     2221 tttacgggca tccagacgct gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc
     2281 tgcctgcgcg ccgctttgca ggaacgcttc cggttcttgc tccagcaggc ccaatacagc
     2341 ggaaagttta cgcaggtgag atgccattgc attcgccgct gccatatctt ctgctttcag
     2401 acggttcact tcacgcgcca tatcaaacag cacggaatag gcttccgggg tgttgaaatc
     2461 gtcgtccatc gcttcaataa agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt
     2521 tttatctgtg ccgcgcagcg cagtgtagag gcgctccagc gccgcacgcg cctgcttcag
     2581 gttctcttcg ctgtagttca gctggctgcg atagtggccc gacatcagga agtaacgcac
     2641 ggtttccgcg tcgtagtatt tcagcacatc gcgcacggta aagaagttac ccagcgattt
     2701 ggacatcttc tcgcggtcaa ccatcaccat tccggagtgc atccagtagt tcacatactg
     2761 accatcatgg gcacaggtgg actgcgcgat ttcgttctcg tggtgcggga acatcaggtc
     2821 tgaaccgccg ccgtggatat caaagtggtt acccagctgc ttgcagttca tggccgaaca
     2881 ttcaatgtgc cagccaggac gacccgcgcc ccacggagac ggccagctcg gttcgccctc
     2941 tttcgacatc ttccacagaa cgaagtccat ccggttgcgt ttgtcatcaa ccacgtcaac
     3001 gcgcgcgcca gcctgcagct ggtcgagatc ctgacgcgac agcacgccat aagttggatc
     3061 ggtcgggacg tcgaacatca cgtcgctgtt gtccgccaca taagcgtgac ctttggcgat
     3121 cagttgttca gtgagttcaa taatttctgc gatatggtgc gtcgcgcgcg gctccatatc
     3181 cgggcgcaga atgttcaaag catcaaaatc tttgtgcatt tcggcgatca tgcgatccac
     3241 cagcgccaca aagctttcgc cattttcatt ggcgcgtttg atgattttgt cgtcgatatc
     3301 ggtaatgttg cgcacatact tcagtttata gccgaggaaa cgcagatagc gcgcaaccac
     3361 gtcaaaagca acaaaggtac gcccgtgacc gatatgacag agatcgtaaa cggtgattcc
     3421 acacacgtac atgccgactt ccccggcgtg aataggctta aattcctctt tttggcgtgt
     3481 cagagtattg aagattttta gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt
     3541 tgcgtataat acccatattt cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt
     3601 gcggttatgc tataacacca ccctatatat gacccgaact gggttgaagc accaatcaaa
     3661 cggaacagga tgcaaaaatg gttactttcc acaccaatca cggcgatatt gtcatcaaaa
     3721 cttttgacga taaagcacct gaaacagtta aaaacttcct ggactactgc cgcgaaggtt
     3781 tttacaacaa caccattttc caccgtgtta tcaacggctt tatgattcag ggcggcggtt
     3841 ttgaaccggg catgaaacaa aaagccacca aagaaccgat caaaaacgaa gccaacaacg
     3901 gcctgaaaaa tacccgtggt acgctggcaa tggcacgtac tcaggctccg cactctgcaa
     3961 ctgcacagtt cttcatcaac gtggttgata acgacttcct gaacttctct ggcgaaagcc
     4021 tgcaaggttg gggctactgc gtgtttgctg aagtggttga cggcatggac gtggtagaca
     4081 aaatcaaagg tgttgcaacc ggtcgtaacg gtatgcacca ggacgtgcca aaagaagacg
     4141 ttatcattga aagcgtgacc gttagcgagt aatcgtggcg acactcttta ttgcagatct
     4201 tcatctctgc gtggaagaac cggcgatcac cgccggtttt ctgcgttttt tagcggggga
     4261 agcccgcaag gccgacgcgc tgtatattct tggcgatctg tttgaagcat ggattggcga
     4321 cgacgatccc aacccactcc atcgccagat ggcggcagcg atcaaagcgg tgtccgattc
     4381 cggcgttccc tgttatttca ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc
     4441 ccgtgaaagc ggcatgacat tattgccgga agaaaaggtg ctcgaacttt atggtcgccg
     4501 ggtgttgatt atgcatggcg acacgctgtg caccgatgac gcgggttatc aggcttttcg
     4561 cgccaaagtc cacaaaccct ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg
     4621 caaacgcatt gccgcgcgaa tgcgcgcgaa cagcaaagaa gccaacagca gtaaatcgct
     4681 ggcgatcatg gacgttaacc aaaacgctgt ggtcagtgcg atggaaaaac atcaggtgca
     4741 atggctaatc cacgggcata cccatcgccc ggcggtgcat gaacttatcg ccaaccagca
     4801 acctgctttt cgcgtggtac tgggtgcctg gcatacggaa ggttcgatgg tgaaagtcac
     4861 ggctgatgac gttgagctga ttcattttcc gttttaaaaa acccacaact ttgctgattt
     4921 cacagccacg caaccgtttt ccttgctctc tttccgtgct attctctgtg ccctctaaag
     4981 ccgagagttg tgcaccacag gagttttaag acgcatgtct tcccgcaata atccggcgcg
     5041 tgtcgccatc gtgatggggt ccaaaagcga ctgggctacc atgcagttcg ccgtcgaaat
     5101 cttcgaaatc ctgaatgtcc cgcaccacgt tgaagtggtt tctgctcacc gcacccccga
     5161 taaactgttc agcttcgccg aaagcgccga agagaacggt tatcaggtga ttattgcggg
     5221 cgcaggcggc gcagcgcacc tgccaggcat gattgccgcc aaaacgctgg tgccggtgct
     5281 gggcgtgcca gtacagagcg ccgcactgag cggtgtcgat agcctctact ccatcgtaca
     5341 aatgccgcgc ggcattccgg tgggtacgct ggcgattggt aaagctggcg cggcaaacgc
     5401 ggcattactg gcagcacaaa ttctcgcgac tcacgataaa gagctacacc agcgtctgaa
     5461 tggctggcgc aaagcccaga ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc
     5521 atgaaacagg tttgcgtcct cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc
     5581 gaaccgttag gcattgctgt ctggccggtc gggctggacg ctgaaccggc ggcggtgcct
     5641 tttcaacaaa gcgtgattac cgctgagatc gaacgctggc cggaaaccgc attaacccgc
     5701 gagctggcgc gtcatccggc ctttgtgaac cgcgatgtgt tcccgattat tgctgaccgt
     5761 ctgactcaga aacagctttt cgataagctc cacctgccga ccgcgccgtg gcagttactt
     5821 gccgatcgca gcgagtggcc tgcggtgttt gatcgtttag gtgaactggc gattgttaag
     5881 cgtcgcactg gtggctatga cggtcgcggt caatggagtt tacgcgccaa tgaaaccgaa
     5941 cagttaccgg cagagtgtta cggtgaatgt attgtcgagc agggcattaa cttctctggt
     6001 gaagtatcgc tggttggcgc gcgcggcttt gatggcagca ccgtgtttta tccgctgacg
     6061 cataacctgc atcaggacgg tattttgcgc accagcgtcg cttttccgca ggccaacgca
     6121 cagcagcagg cgcaagccga agagatgctg tcggcgatta tgcaggagct gggctatgtg
     6181 ggcgtgatgg cgatggagtg ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggct
     6241 ccgcgtgtgc ataacagcgg tcactggaca caaaacggtg ccagcatcag ccagtttgag
     6301 ctgcatctgc gggcgattac cgatctgccg ttaccgcaac cagtagtgaa taatccgtcg
     6361 gtgatgatca atctgattgg tagcgatgtg aattatgact ggctgaaatt gccgctggtg
     6421 catctgcact ggtacgacaa agaagtccgt ccggggcgta aagtggggca tctgaatttg
     6481 accgacagcg acacatcgcg tctgactgcg acgctggaag ccttaatccc gctgctgccg
     6541 ccagagtatg ccagcggcgt gatttgggcg cagagtaagt tcagttaagc aggtgctcat
     6601 ttgattgccg gatgcggcgt aaacgcctta tccggcttac cgctccggta cccattgtag
     6661 gcctgataag acgcgtcaag cgtcgcatca ggcatggtgc accgattgcc ggatgctctc
     6721 atcactacag cgaaatacag gtccccgctt cgcccgccag cgtctcttca attcgcgata
     6781 acgccccaat ccacgcgggt ttactacggc ttctgacata accactcacc gccgttacct
     6841 tcggccccat cgaaccatcg gctttggcaa atggcgctaa ctcatccggt gtggcatggc
     6901 gaatggcacg ttgctgcggc gttccccagt tttcatatac cgcatcagca tcggtgagga
     6961 tcaccagtcc atcggcatta atctgctcgg cgagcaacgc agcggcgaga tctttatcaa
     7021 tcaccgcttc actccctgcc ccatcttccg tcacaggcac accgccgccg ccactgcaaa
     7081 tcaccacatg cccctctttg agcaacaact cgatggcttc gctgtcgaga attttacgcg
     7141 gttgcggaga cgccaccacc cggcgcaaat atttaccatc acgtttcatc tgccagccgt
     7201 aagccgcttc cagtgcctct tgttcttctg gctgataaac cggaccaata aatttctctg
     7261 gctgcaaaaa cgccggatca tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtaacag
     7321 gcggcatctg cggctgtgcg ctcaagctct gcgccagcat atagccaatc atcccctggc
     7381 tttccgccac cagtacatcc agcggatacg gttctacctc tttccacgcc agattctgta
     7441 acgccagcag ccccacctgc ggcccgttgc cgtgaacaat cgccagccga taagaacggg
     7501 ccaggcgtgc cagcgcgggt acagcactgg cgatattgcg atattgattt tccgccgtca
     7561 gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc tacaaccagt gttttcatgg
     7621 ttttccttgt aataattgtt ggccaagcca gaaccccagc agcgtatctg cccctgacgt
     7681 atgccccagc gccagcagcg aatcaatcgc aacagcggta cgtttcgggc aactcagagc
     7741 atggacaaag tgcaggagtg gcgaggcgaa atatccttgc gcggcataac gtaaataact
     7801 gacgctcacc gctgtggtaa cgagttgaag attgtcggaa caggcaaaaa acgggcgacc
     7861 ggcgcgcgca tctaaagcgc cataatacca ggccgccagc agcattccgc tcaacgtgtc
     7921 atcatgactt ggcgttaatc cggggccttt acccagccag tgccgccagt cggtcttaac
     7981 gccattgagc gcggcctgaa aacagtgacg aaactggcgt aattcagcgg gcagcggatc
     8041 gcttgccgcc atcgccagtg gcccgaaaag tccggtttcc tccgcgcgtt gcatccatgc
     8101 agctgcaagc ggttgaggat gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg
     8161 ctgtttaacc gtgaaacgcc cgaggcgaat cccttgagcc acaacctgtg gtcgttcatt
     8221 gccgcataat ccaccgcata acgcatcgaa ttgcgtgcgg cgaaattaca cattcccgtc
     8281 aagctataga agaaacgctg ccctggcttt cctcttttgg cgctgaccca acgggtggga
     8341 tgacccgttt actttattcg ccggaatggc tggaaaccca gcagcaattt aaaaaaagaa
     8401 tggcagcaag tgggctggaa acacgtttcg atgaagtggg gaatttatac ggtcgcctga
     8461 gtggcaccga atatccacag gaagtggttc tgagcggttc gcatatcgat accgtggtta
     8521 acggcggtaa cctcgacggg caattcggcg cgctggcggc gtggctggca attgactggt
     8581 tgaaaacgca atacggcgca ccgttacgta cggtcgaagt ggtggcgatg gcagaagaag
     8641 aaggcagccg cttcccgtat gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc
     8701 ctgacgacgt gcggaatatc tgtgatgcca aaggaaatag ttttgccgat gcgatgaagg
     8761 cttgcggatt tactctgccg aacgccccac taactccgcg tcaggctata aaagcctttg
     8821 ttgaactgca tattgaacag ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg
     8881 tgaatgcaat tgtcgggcag cgtcgctata cggtgacgct gaacggcgaa tcaaaccatg
     8941 caggcaccac gccgatgggt tatcgtcgtg atacggttta cgctttcagt cgcatttgcc
     9001 atcagtcgat cgaaaaagcg aaaaagatgg gcgatccgct ggttctgacc tttggcaaag
     9061 tagagccgcg cccgaatacg gtaaatgtgg tgccgggtaa aaccacgttc accattgatt
     9121 gtcgtcatac cgacgctgcc gtgctgcgcg atttcaccca acagttagaa aacgacatgc
     9181 gggcgatttg cgatgaaatg gacattggta ttgatatcga tttatggatg gacgaagaac
     9241 ccgtgccgat gaataaggag ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac
     9301 tgaattaccg ggtgatgcac agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg
     9361 taccaacctg catgattttt atccccagca tcaacgggat cagccataac ccggcggaac
     9421 gcaccaatat tactgacctt gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac
     9481 ttgcctggca ggaataagga gtcataaatg ggatatttaa ataacgtcac cggttaccgc
     9541 gaagatttac tggctaaccg tgcgattgtt aaacacggta atttcgcact gttaaccccg
     9601 gatggtctga taaaaaatat tattccggac tttgaaaatt gtgacgcgac aatcctctcc
     9661 acgccaaagc tgggtgcctc ttttgttgat tatctggtca cactgcatca aaacggtggc
     9721 aaccaacagg gcttcggtgg cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat
     9781 atcactgcca aagccgaagg caaaacattt gccttaagcg aaggtggcta tctttattgc
     9841 ccaccaggct ccttaatgac gtttgttaac gcccaggccg aagacagcca aatcttttta
     9901 tataagcgcc gctatgttcc ggtagaaggc catgcaccgt ggctgttttc tggcaatgcc
     9961 agcgaactgg aacgcattca ctatgaaggt atggatgatg tgatcctgct ggattttctg
    10021 cccaaagagt taggttttga tatgaacatg catatcctct cttttgcacc aggtgccagc
    10081 cacggttata tcgaaacaca cgttcaggaa catggtgcct atattctttc cggtcagggg
    10141 gtttataac
//
LOCUS       DBBBSB010000137        10139 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.137, whole genome shotgun sequence.
ACCESSION   DBBBSB010000137 DBBBSB010000000
VERSION     DBBBSB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.137"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            156..245
                     /locus_tag="ACIUS0_003498"
                     /pseudo
     CDS             156..245
                     /locus_tag="ACIUS0_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            547..1542
                     /gene="pgl"
                     /locus_tag="ACIUS0_003499"
     CDS             547..1542
                     /gene="pgl"
                     /locus_tag="ACIUS0_003499"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGB0221096.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1583..2536)
                     /locus_tag="ACIUS0_003500"
                     /pseudo
     CDS             complement(1583..2536)
                     /locus_tag="ACIUS0_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2720..3772
                     /locus_tag="ACIUS0_003501"
     CDS             2720..3772
                     /locus_tag="ACIUS0_003501"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGB0221097.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3848..5281
                     /locus_tag="ACIUS0_003502"
     CDS             3848..5281
                     /locus_tag="ACIUS0_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGB0221098.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5464..7644
                     /locus_tag="ACIUS0_003503"
     CDS             5464..7644
                     /locus_tag="ACIUS0_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGB0221099.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7785..9068)
                     /locus_tag="ACIUS0_003504"
     CDS             complement(7785..9068)
                     /locus_tag="ACIUS0_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGB0221100.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9203..>10139)
                     /locus_tag="ACIUS0_003505"
     CDS             complement(9203..>10139)
                     /locus_tag="ACIUS0_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221101.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2465 a         2364 c         2557 g         2753 t
ORIGIN      
        1 tagtaatgat caaaatcata tctcaatgta ctgttttttt ctttaaaaaa acagtacatt
       61 ctattttata atgactgtgt attcccatgg aaggagatca cccaattctc ccccccgact
      121 tttgacgcag tgtcttgtta acagggggat tgaccatgct tattggctat gtacgcgtat
      181 caacaaatga ccagaatacc gatctacaac gtaatgcgtt tatttgtgcc ggaagtgagt
      241 tgattgttgc gcgaatggga agttggccag aagttgttat ggcatagtgt ttacggtcgt
      301 tgatacaggt aaacaaaagc ggggagagtc atactccctg aatagtcgcg aaaatatctg
      361 tggcattgtc aaattcgtgt cggtgactaa tattttagta atatgccatt cgaaatggag
      421 tgctaactta ttgatttaat tgatgtgcga tattgtattt tatcgtgttt tgttgtctgt
      481 tttttatcca gtaagtgttg ttatatgtat gaaagataag ggatttatca tttttgaggt
      541 gtgttcatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
      601 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
      661 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
      721 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
      781 gtgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
      841 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
      901 gtcgtcgatg tagtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
      961 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     1021 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccgttgaagg ggccggcccg
     1081 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     1141 tcagtggatg tctgggaact gaaagatccg cacggcaata tcgaatgtgt ccagacgctg
     1201 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     1261 gatggtcgcc atttatacgc ctgcgaccgt accgccagtc tgattaccgt tttcagcgtt
     1321 tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     1381 ggcttcaatg ttgattacag cggcaagtat ctgattgccg ctgggcaaaa atctcaccac
     1441 atctcggtat acgaaattgt tggcgagcag gggctactgc atgaaaaagg ccgctatgcg
     1501 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     1561 cagtctctcc tgcgccggtg tattaactta tctcctgtaa cgcatgtctc tggcgttcga
     1621 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggaaaaaa
     1681 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     1741 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     1801 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     1861 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggctagc tgttgttcta
     1921 tcaacaatcg gttgccgctg gaggaacgta cgccaatcat tttgtaaccc actaactcct
     1981 gccattctac aagctacttt ttggccaatg gatggtcacg ccgacaggcc agcacgaatg
     2041 gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc atgtttatgc
     2101 caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct tgttccagaa
     2161 tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc ggtaaaaaat
     2221 aaaatactgc ggtcggaata cacgctaatg tcaccatacc acgatgataa gcattcatat
     2281 cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa ttcccgcgcc tccgggagca
     2341 acctttttcc tgcttttgtt aacgtaacct tgcgagttgt acgctcaaat agttgaatat
     2401 gtaaatcttc ctccatcttt tttattcttc gcgttaatgc aggttgcgta atattgagta
     2461 atttagcggc attgttaaat gaactggact ctgccagtat gacaaatgcc ttcatacttg
     2521 ataattcatg cttcattatt actccggaaa atggaagcga cgattttggg gggctggtcg
     2581 ttaaaaattt taactgcatt tagccaactt aaattaatga aaaaatgtta ttaatcgttg
     2641 agctaaagtc attagagatg ctttgtcctt aatgtaacca tatcgcaata agttatgttt
     2701 ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag gtggaacctc
     2761 gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc gcgataaaat
     2821 attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg gcggcggtaa
     2881 tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg ctgatgtcga
     2941 ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc ctaactgcgg
     3001 caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg cagcgacttc
     3061 gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg aagctgatgt
     3121 gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg gcgtaccggg
     3181 tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa ctggaaaagt
     3241 tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct gtatcgatat
     3301 ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt atgaattacc
     3361 ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc ggctacaagc
     3421 gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac ctgtgcttat
     3481 ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc attcttgcca
     3541 tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat tggaaggcac
     3601 cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg aacaccccag
     3661 tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt tacgcgcatc
     3721 tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct gaaaaaattc
     3781 gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag aaagaacagg
     3841 attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga tcccatgtat
     3901 tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc tttttggtat
     3961 ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg tcgtactgtt
     4021 aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg cgtttaagac
     4081 caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct cggcgtttac
     4141 cttaagcgcc gcatttgtga ccaccggttt aggtaaactt attgcctatc tgctgattgg
     4201 taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg atctggtact
     4261 ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac cgatcatcaa
     4321 cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg tcggacatta
     4381 cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct ttaccgcaat
     4441 ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc aaattagctg
     4501 gggtggatgg gcgctggccg ccggattgcc gggcatcatt atgctgctgg tcaccccgct
     4561 ggtaatttac accatgtatc caccagaaat taaaaaggtg gataacaaaa ccatcgctaa
     4621 agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa aaaatgctgc tcggtgtctt
     4681 tgtgctggcg ctactgggct ggattttcag taagtctctg ggggttgatg aatccactgt
     4741 ggcaatcgtt gttatggcga ccatgctgct gctgggtatc gttacctggg aagacgtggt
     4801 taaaaataaa ggcggctgga ataccttaat ctggtacggc ggtattatcg gcttaagctc
     4861 cttattatcg aaagttaaat ttttcgaatg gttagctgaa gtctttaaaa ataacctggc
     4921 atttgatggt cacggtaacg ttgctttctt cgttattatt ttcctcagca tcatcgtgcg
     4981 ttatttcttc gcttccggta gtgcctatat cgttgctatg ttacctgtat ttgccatgct
     5041 ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg gcactgttgt tctccaactc
     5101 ctatggcggc atggttactc actatggcgg cgcggcaggt ccggtcatct ttggcgtggg
     5161 ttacaacgat attaaatcct ggtggttggt cggtgcggta ctgacgatat taaccttctt
     5221 ggtgcatatc accctcggcg tgtggtggtg gaatatgctg atcggctgga acatgctgta
     5281 aatatactcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca ccccagtcac
     5341 ttacttatgt aagctcctgg ggattcactc acttgtcgcc ttcctgcaac tcgaattatt
     5401 tagagtatat ccatttatta tctttctgcg cacttcacgg tgcgcagata tctggagcat
     5461 ttgatgatca agttatctga aaaaggcgtg tttctcgcca gtaataacga aataattgcc
     5521 gaagaacatt tcaccggcga aattaaaaaa gaagaagcaa aaaaaggcac tattgcctgg
     5581 tctattctct cttctcataa tacgtccgga aatatggata aacttaaaat taagtttgat
     5641 tcattagcct ctcacgatat tacctttgtt ggtattgtac agaccgctaa agcgtccgga
     5701 atggaacgtt tcccgctgcc gtatgtgctg accaactgcc ataactcact ctgcgccgtc
     5761 ggcggcacca ttaacggtga tgaccatgtt tttggtttat cggcagctca gcgttatggc
     5821 ggtatttttg tgcctccgca tattgcggtc atccatcaat atatgcgtga gatgatggct
     5881 ggcgggggca aaatgatcct cgggtcagac agccacaccc gttacggtgc attagggaca
     5941 atggcagtcg gtgagggtgg cggtgagttg gtaaaacagc tgcttaatga cacctgggat
     6001 atcgactatc cgggcgtggt tgcggtgcat ctgaccggaa aacctgcgcc gtatgtgggg
     6061 ccacaggatg tggcgctggc tatcattggc gcggtgttca aaaacggtta cgtcaaaaac
     6121 aaagtcatgg agttcgttgg accgggcgtt agcgcgctct ctaccgattt ccgtaacagc
     6181 gttgacgtga tgaccacgga aaccacctgt ttaagctccg tctggcaaac tgatgaagaa
     6241 gtgcataact ggctggcgct gcacggtcgc ggtcaggatt actgccagct taaccctcaa
     6301 ccgatggcgt actacgatgg ctgcatcagc gttgatttaa gcgccatcaa accaatgatt
     6361 gcgctgccgt tccacccgag caacgtgtat gaaatcgaca cactgaacca gaacctgacc
     6421 gacattctgc gtgagattga aattgagtcc gaacgcgtgg cgcacggtaa agccaaactc
     6481 tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc agcaggggat tatcgcgggc
     6541 tgttctggcg gtaactacga aaacgtcatc gcggcggcga atgcactgcg cggtcaatcc
     6601 tgtggcaatg acaccttctc gctggcggtt tacccgtcat cacagccggt gtttatggat
     6661 ctcgccaaaa aaggtgtggt agcagatttg attggcgcag gcgcaatcat cagaaccgca
     6721 ttctgtggcc catgctttgg cgcgggcgat acgccaatca acaacggttt gagtattcgc
     6781 cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc cagccaatgg gcagatgtca
     6841 gcggtggcgt tgatggacgc tcgttctatc gctgcgactg cggcaaacgg tggctattta
     6901 acctctgcca gcgaacttga ttgctgggac aacgtgccgg agtacgcctt cgatgtaacg
     6961 ccgtataaaa accgtgttta tcagggcttt gtgaaagggg caactcagca accgctgatt
     7021 tacggaccga acattaaaga ctggccggaa ttgggtgcgc tgactgacaa tatcgtcctg
     7081 aaagtgtgct cgaagatcct cgacgaagtc accaccaccg acgaactgat tccttccggt
     7141 gaaacctctt cttatcgttc aaatccgatt ggtctggcgg ggaatgtcag cgagctgaca
     7201 gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact
     7261 gaaattggca gcatggtcta tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg
     7321 gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg ccgaggagta cgcgactaaa
     7381 cgctaccgtt ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca
     7441 acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg
     7501 ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctt
     7561 tacatggaaa gcctgacggc tgaagagcgc gagattatca aggcgggtag tttgattaac
     7621 ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt
     7681 attgtcggat gcgatgctgg cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc
     7741 cggataaggc gtttacgccg catccggcaa atagttaata gctcttactt cttctcctct
     7801 gcaaccactt tgctgcccac gccgcggtta ttgtattccc acatgcggtt gtagttagtg
     7861 tcattcagat tgcgctgtat ttcgtcgtta tcatctacgc tgccggtatt acccgcaaac
     7921 ggacgattag agatcaccgc atcggcccac ggtttagccg tgttaaaacc ttcgttgatg
     7981 gcgctatcac ggatcaccac ctgaccgttg gtattagcat caacatccag cgagcggccc
     8041 agttgcgcca cgccatcacc ggaagcattg aaacggctgt ttacggcgag gaaaccgtaa
     8101 tagatgttag acagcgtagc cggtgcaaac acatatgctt cttgctgggt acgtgagttc
     8161 accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac gatatccaca
     8221 tccccttcaa tatagctgtt ggtcaccagc gtacgcggct gacggttggt ttccagacgg
     8281 ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat gttaacgttg
     8341 ttaatctgta cttggtcacc gtcagtacgc agtgctaccg ccggatggtt acctgcatct
     8401 acgctatcgc ccagcgtgtt ttcgatggtc agattttgca gttgcagtcc attgttttgt
     8461 gaccagaaga ccgcagaaca cagaacaccg atactgtcgc tgcgtttgct ctggcagcta
     8521 tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt gacgtcgtga
     8581 cgccagtcgg cagggctcat gcccccatca agggaaagcc caatcttcac atcaatcggt
     8641 ttttcacctg taccgtacag agtgattcca cctggagcag cagggacata caccgttccc
     8701 tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtgcgctt gataattgcc
     8761 gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtacccgc cgggccgaca
     8821 acaaagtcag gttgcgcagg cagggttatc ggggacggat tccacgctgc agcacctggt
     8881 gtcagggatg caaaatagtg ttgcgcatcg aaattcagcg cttcttttgc cgacagaatc
     8941 gggcgagaag aggtaccagg cgcagtttga tcagaaggac gttgatcggg tggtgttgag
     9001 ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga aactgaaaat
     9061 gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa gcctgctttt
     9121 ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     9181 attatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc ctgcctctgt
     9241 catcacgata ctgtgatgcc atggtgtccg acttgtgccc gagaagatgt tgagcaaact
     9301 tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg tgaaaggtag
     9361 gtggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa taccttgata
     9421 ctgtgccgga tgaaagcggt tcacgacgag tagatgcaat tatggtttct ccgccaagaa
     9481 tctctttgca tttatcaagt gtttccttca ttgatatccc gagagcatca acatgcaatg
     9541 ttgtagggat ggcaattttt acgcctgttt tgctttgctc gacataaaga tatccatcta
     9601 cgatatcaga ccacttcatt tcgcataaat caccaactcg ttgcccggta acaacagcca
     9661 gttccattgc aagtctgagc caacatggtg atgattctgc tgcttgataa attttcaggt
     9721 attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga gtggcagcga
     9781 ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg
     9841 atctgattaa cttggctgat gccgccttgc cctcgtctat gtatccattg agcattgccg
     9901 caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagccccctc cttattgctt
     9961 taattttgct catgtaattt atgagtgtct tctgcttgat tcctctgctg gcgaggattt
    10021 tttcgtagcg atcaagccat gaatgtaacg taacagaatt atcactgttg attctcgctg
    10081 tcagaggctt gtgtttgtgt cctgaaaata actcaatgtt ggcctgtata gcttcagtg
//
LOCUS       DBBBSB010000138        10009 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.138, whole genome shotgun sequence.
ACCESSION   DBBBSB010000138 DBBBSB010000000
VERSION     DBBBSB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.138"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..570
                     /locus_tag="ACIUS0_003506"
     CDS             <1..570
                     /locus_tag="ACIUS0_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HGB0221102.1"
                     /translation="KRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRK
                     LYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMR
                     RYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYI
                     EYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            667..819
                     /gene="hokE"
                     /locus_tag="ACIUS0_003507"
     CDS             667..819
                     /gene="hokE"
                     /locus_tag="ACIUS0_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGB0221103.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            1170..1322
                     /gene="hokE"
                     /locus_tag="ACIUS0_003508"
     CDS             1170..1322
                     /gene="hokE"
                     /locus_tag="ACIUS0_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGB0221104.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1444..2064)
                     /gene="entD"
                     /locus_tag="ACIUS0_003509"
     CDS             complement(1444..2064)
                     /gene="entD"
                     /locus_tag="ACIUS0_003509"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGB0221105.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(2239..4479)
                     /gene="fepA"
                     /locus_tag="ACIUS0_003510"
     CDS             complement(2239..4479)
                     /gene="fepA"
                     /locus_tag="ACIUS0_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGB0221106.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4722..5924
                     /gene="fes"
                     /locus_tag="ACIUS0_003511"
     CDS             4722..5924
                     /gene="fes"
                     /locus_tag="ACIUS0_003511"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGB0221107.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5927..6145
                     /gene="ybdZ"
                     /locus_tag="ACIUS0_003512"
     CDS             5927..6145
                     /gene="ybdZ"
                     /locus_tag="ACIUS0_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGB0221108.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            6142..9987
                     /gene="entF"
                     /locus_tag="ACIUS0_003513"
     CDS             6142..9987
                     /gene="entF"
                     /locus_tag="ACIUS0_003513"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGB0221109.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2235 a         2589 c         2734 g         2451 t
ORIGIN      
        1 aagcgatacc gctcttacag aggagaggta gggcaaaccg cccctaatgt tctccaaaga
       61 gatttcaagg ctacgcggcc aaacgagaag tgggttaccg atgttactga atttgcagtc
      121 aatgggcgca agctgtattt gtctccagta atagatctct tcaacaacga agttatttct
      181 tacagccttt cggaaagacc agtgatgaac atggttgaga atatgctcga tcaggcattc
      241 aaaaagctta atcctcacga gcatcctgtt ctgcactctg accagggatg gcagtatcgt
      301 atgagaagat atcaaaatat ccttaaagaa catggtatta aacaaagcat gtccagaaaa
      361 ggcaattgtc tggataatgc tgtggtggag tgtttctttg gaaccttaaa gtcggagtgt
      421 ttttatcttg atgagttcag taatataagc gaactgaagg atgctgttac ggaatatatt
      481 gaatactata acagcagaag aattagcctg aaattaaaag gtctgactcc aattgaatat
      541 cggaatcaga cctatatgcc tcgtgtataa ctgtccaact ttttggggtc agtacaccct
      601 ggggctgcca ctccaaacca gacaatttgg atggtagtcc cttcttcgca tggaggtaat
      661 ataaacatgc tgacgaaata tgcccttgtg gcagtcatag tgctgtgttt gacggtactg
      721 ggatttacgc ttctggccgg agactcgctg tgtgagttta cggtgaagga acgtaatatt
      781 gagttcaggg ctgttctcgc ttacgaaccg aagaagtagc cgttgtgcgg ggagtaatcc
      841 ccgcatatcc ggttgtcagg tcaggatggt aaggcacctg ctttacactt tcgcccgcgg
      901 tcagtgatgg ctgcgggcga tttcgtttta agagcaatga tgagttttgc cgacagaagt
      961 ccgaaacagg actcactgta taaataaaca gctattttgc taaggaaggg taagataacg
     1021 gcgggtgcct gaagctttcc ggtttcaggt taactctgag gtttggaaag atgaagcccc
     1081 aagagatatt tctatcaatc ctggggctgc cactccgaac ctcaacaact cggatggtag
     1141 tcccttcttc gcatggaggc aatataaaca tgctgacgaa atatgccctt gtggcagtca
     1201 tagtgctgtg tttgacggtg ctgggattta cgcttctggt cggagactcg ctgtgtgagt
     1261 tcaccgtaaa ggaacgtaat attgagttta aggctgttct tgcttacgaa ccgaagaagt
     1321 agccgttgtg cggggagtaa tccccgcata tccggttgtc aggtcagagt ggtaaggcac
     1381 ctgctttaca ctttcgcccg cagtcagtga tggctgcggg cgaatcgtac cagatgttgt
     1441 caattaatcg tgttggcaca gagttatgac tattttttct tttatctgcc agtgcacagc
     1501 aaacatctca ttctcacgat gaatgatgac ctgctgttta ttccagctaa ttatctgata
     1561 gtccagaaaa cctgcatcag tttggatctc acttgcctta aatgcgctct ctttggcgga
     1621 aaatgcaagt gtcagcgcca gagaaaaggc taaaccgcag tctgcgagtc gctcgtgttc
     1681 tgctggtgta ataatgttgt ctgtcaattc ggtcgctgtt tgcgcagaaa aaatttcttc
     1741 gatatcaacg ccaatcggtt gacgggaaac cacggccaat gccgtagccc cgcagtggct
     1801 aatactgccg tatacctccg caggccacac cggttggcgt agctcgccga ttgcgggcac
     1861 acatttatag ccatattccc gcaaggcata aacagcagcg atccgtccgg ctaaatgctc
     1921 tgttttacgt ttacgtccag cgtgttgcag ttgtgcgtag tgcggcagcc agagtaaatc
     1981 ctgctcacaa aaattcgccg gatcgaactc aacaaaatgc agcgtatgtc cggcaaaggg
     2041 gagggaggta tgcgtagttt tcatatcgac catattcgag actgatgaca aacgcaaaac
     2101 tgcctgatgc gctacgctta tcaggcctac gtgatcctgc aatatattga attggcaaga
     2161 tctttgtagg ccggataagg cgttcacgcc gcatccggcg tgaacgacgc gcactttgtc
     2221 aacaatctga cgttagcatc agaagtgagt attaatgctc atataccacg tacgtcccgg
     2281 ctcgttatag gtatacgcgc cggcaccggc gatatagttg gctcctgcca gatcgcccgt
     2341 ggtctgggca ttacctgcac gccacaaacg tttgtcgaac aggttgtcca cgccgccggt
     2401 cagactgaca ttcttcgtca catcccaggt cgcgctcaga cccacaatgc tgtaaggact
     2461 gatttctttg gtttccgggc caaccgctgg ctgaccttta tagttgtact tcttcggctg
     2521 ctgcttgccg taccaggtga aggtcgtttg catcgacaaa tcttcccgtg cctgccagct
     2581 cagcgttgag ttcaacgtgt actccgggat gatcgacaaa cggtcgcccg tggttttgtt
     2641 ttcactcttc agcatataag tgatgttatt ggtccacatc accgtttcgc taactggtac
     2701 gtttaacgat ccttccagac cttcaaccac tgctttcggc acgttatccc actgatagag
     2761 atcagtgccg actgcgtttt gccctacagc cacatagccc gcttcaatct tattgcgata
     2821 atcgttacgg aaccaggtga cgcccgccag ccagccgtcg cgtttgaact ccagaccaat
     2881 ctctttgttg atgctggttt ctactttcag gtcatcgtta ccttgcagat agcagccgcc
     2941 cgcgctggca tagcagccct gacctttact gtagagaatg tagttcgggt tagtctggta
     3001 taggctcggc gctttatagg cgcgggcaat gcctattttc agcgtgaagt catcgcctaa
     3061 accttgcgat atgttcagcg ccgggctcca gttattgccg acaatactgt gatgatcgaa
     3121 acgcagcccc ggcgttacga tggtgctgtc agtcagctcc atgttgtttt cggcaaacag
     3181 cgagaaaatt tctgcttttg aatacgggct acggtcggtg gcactcacgc catcaatagc
     3241 gccaccggta ttcgttccgg tcagtgcttg ggtgttggaa cttaagtcct tcatccgttg
     3301 ctgattccac tccgtaccca gcgtcagcgt ctggttaacg aggaaatcaa tcggcaggtt
     3361 aacttcgctg tgcagcatca cgtcatcgag atcgatatcg acgaaatcct gtgtcgcttt
     3421 ttcgttaaat ttcccttcgg taccgcccgc cagaccttcc ggaatacgcg agttacgggt
     3481 gtgttcatac tgcacccagt tgctggtggt cacgccgtta tcccagccac cgttccaggt
     3541 cagcgcgtag ttctggcgat acaggcggtt ggtttcatcg ccatatttcg agcgagtgta
     3601 agcgtcagag ttggtgttct gggtatcgcc cgcatacagg ttaccctggc ggctgtaacc
     3661 cgcttccagt tccagcgatt gcaatggtgc gaagtcccag cgcaccacgc cgttaatatc
     3721 tttattgatc accccttcgc gcccggctgg taacgtcgtg gcatacgttc cggcacgcgc
     3781 ggactgatgg ccctggttga tatcccacgc gtcagcctga gttttgtcga ggttgccata
     3841 caagcggaag ctgaattcgt cgcccagcgg accggtcagg ctaaagttag tgcgtttggt
     3901 ggcaccttcc tctttatgtt ctggcgcatt gaaatatgcg tcccaggagc cgtgccactc
     3961 gccgctgcct tttttggtaa tgatgttaac cacgccgccc gccgcgccgt tgccataacg
     4021 cgcagctgcc ggaccacgca gaacttcaat acgttcaatc atttcaggtg gcacccagga
     4081 ggtatcacca cgggtatcgc gctcgccacg ccagccctga cgcaccgagt tacggctgct
     4141 taccggcttg ccgtcaatca aaatcagcgt gttttccgga cccataccgc gaatatcaat
     4201 ctgtcggtta ttcccacgct gaccactggt ggagttaccg gtcaggttaa cgcctggcat
     4261 ggtacggatg atctccgaca catcgcgggc aaccgggttt ttgcggattt catctgcggt
     4321 gatggtcgaa acgccaggag cctgcaagtt ctgctcggcg gcggtaacga caatggtatc
     4381 gtcatgtgaa acaggtgtat cggtcggctc ttgtgcctgc gctaccccat aaatccccag
     4441 attgaccaac aaggccaggg aatgaatctt cttgttcatt gttttattcc tgcatttttg
     4501 ccacgaattg caactgtcgg gcatggtcgt catcaacacg acgcatcccg ttaccgcgaa
     4561 aacctttgat cctgaaagac acgcagtgca gttggttaat taatgtccgc gcttcccacg
     4621 gcgcgccatt acgctattgc aaatgcaaat agttattaat aacattatca atatatttct
     4681 gcaatcaatg aaaaattgca cagtaaacat ggggttatgg tgtgacggcg ttaaaagtag
     4741 gaagtgagag ctggtggcag tcgaaacatg gcccggaatg gcagcgtctg aatgacgaaa
     4801 tgtttgaggt cactttctgg tggcgtgatc cccaaggttc tgaagaatac tcgacgataa
     4861 agcgcgtatg ggtctacatc actggtgtga ccgatcatca tcagaacagc cagccccagt
     4921 cgatgcagcg aattgcaggt actgatgtct ggcagtggac gacacaactc aatgccaact
     4981 ggcgcggcag ctactgcttt attcccaccg aacgcgatga cattttttct gcaccatccc
     5041 ccgatcgcct cgaattacgt gaaggctggc gaaaactatt accccaggca atagccgatc
     5101 cgctgaatcc acaaagctgg aaaggcgggc tggggcacgc tgtttctgca ctcgaaatgc
     5161 cgcaagcgcc tctgcaaccg ggatgggatt gtccgcaagc gccagaaata ccagccaaag
     5221 aaattatctg gaaaagtgaa cggttgaaaa attcacgtcg tgtatggatt tttaccaccg
     5281 gcgatgtaac agcagaagaa cgcccgctgg cagttttgct cgatggcgaa ttttgggcgc
     5341 aaagtatgcc cgtctggcct gtgctgactt cgctgaccca tcgtcagcaa cttcctcccg
     5401 ccgtgtatgt gttgatcgac gctatcgaca ccacgcaccg cgcccacgaa ctgccgtgta
     5461 atgcggattt ctggctggca gtacagcaag agttattacc cctggtgaaa gttattgccc
     5521 cttttagcga tcgcgccgat cgcaccgtgg tcgccgggca gagttttggt gggctgtccg
     5581 cgctgtatgc cggactgcac tggcctgaac gctttggctg tgtattaagc cagtcaggat
     5641 cgtactggtg gccgcatcgg ggcgggcagc aagagggcgt gttacttgaa aagctaaaag
     5701 ctggtgaagt tagcgccgaa ggtctgcgca ttgtgctgga agcgggtatt cgcgagccga
     5761 tgatcatgcg ggccaatcag gcgctgtatg cgcaattaca ccctataaaa gaatccattt
     5821 tctggcgtca ggttgacggc ggacatgatg cgctttgttg gcgcggtggc ttgatgcagg
     5881 ggttaatcga cctctggcaa ccacttttcc atgacaggag ttgaatatgg cattcagtaa
     5941 tcccttcgat gatccacagg gagcgtttta cattttgcgc aatgcgcagg ggcaattcag
     6001 tctgtggccg caacaatgcg ccttaccggc aggctgggac attgtgtgtc agccgcagtc
     6061 acaagagtcc tgccagcagt ggcttgaagc ccactggcgt actctgacac cggcgaattt
     6121 cacccagttg caggaggcac aatgagccag catttacctt tggtcgccgc acagcccggc
     6181 atctggatgg cagaaaaact gtcggattta ccctccgcct ggagcgtggc gcattacgtt
     6241 gagttaaccg gagaggttga tgcgccatta ctggcccgcg cggtggttgc cggattagcg
     6301 caagcagata ctttgcggat gcgttttacg gaagataacg gcgaagtctg gcaatgggtc
     6361 gatgatgcgc tgatattcga actgccagaa attatcgacc tgcgaaccaa tattgatccg
     6421 cacggtactg cgcaggcatt aatgctggcg gatttgcaac aagatttgcg cgtcgatagc
     6481 ggtaaaccac tggtctttca tcaactgata caggtggcag ataaccgctg gtactggtat
     6541 cagcgttatc accatttact ggtcgatggc ttcagttttc cggccattac ccgccagatc
     6601 gccaacattt actgcgcatt gctgcgtggc gaaccaacgc ctgcttcgcc gtttacgcct
     6661 ttcgctgatg tagtggaaga gtaccagcaa taccgcgaaa gcgaagcctg gcagcgtgat
     6721 gcggcattct gggtggaaca gcgtcgccaa ctgccaccgc ccgcgtcact ttctccggca
     6781 cctttagcgg ggcgcagcgc ctcggcagat attttgcgtc tgaaaatgga atttaccgac
     6841 ggggaattcc gccagctggc tacgcaactt tcaggtgtgc agcgtaccga tttagccctt
     6901 gcgctgacgg ctttgtggct ggggcgattg tgcaatcgca tggactacgc cgccggattt
     6961 atctttatgc gtcgactggg ctcggcggcg ctgacggcta ccggacccgt gctcaacgtt
     7021 ttgccgctgg gtattcatat tgcggcgcaa gaaacgctgc cggaactggc aacccgactg
     7081 gcaacccgac tggcagcaca actgaaaaaa atgcgtcgtc atcaacgtta cgatgccgaa
     7141 caaattgtcc gtgacagcgg gcgagcggca ggtgatgaac cgctgtttgg tccggtactc
     7201 aatattaagg tatttgatta ccaactggat attcctggtg ttcaggcgca aacccatacc
     7261 ctggcaaccg gtccggttaa tgaccttgaa ctggccctgt tcccggatga acacggtgat
     7321 ttgagtattg agatcctcgc caataaacag cgttacgatg agccaacgtt aatccagcat
     7381 gctgaacgcc tgaaaatgct gattgcccag ttcgccgcgg atccggcgct gttgtgcggc
     7441 gatgtcgata ttatgctgcc aggtgagtat gcgcagctgg cgcagatcaa cgccactcag
     7501 gttgagattc cagaaaccac gctgagcgcg ctggtggcag aacaagcggc aaaaacaccg
     7561 gatgctccgg cgctggcaga tgcgcgttac ctgttcagct atcgggaaat gcacgagcag
     7621 gtggtggcgc tggcgaatct gctgcgtgag cgcggcgtta aaccagggga cagcgtggcg
     7681 gtggcactac cgcgctcggt ctttttgacc ctggcactcc atgcaatagt tgaagctggc
     7741 gcggcctggc taccgctgga taccggctat ccggacgatc gcctgaaaat gatgctggaa
     7801 gatgcgcgtc cgtcgctgtt aattaccacc gacgatcaac tgccgcgctt tagcgatgtt
     7861 cccaatttaa ccaacctttg ctataacgcc ccgcttaccc cccagggcag tgcgccgctg
     7921 caactttcac aaccgcacca cacggcttat atcatcttta cctctggctc caccggcagg
     7981 ccgaaagggg taatggtcgg gcagacggct atcgtcaacc gcctgttgtg gattcaaaat
     8041 cattatccgc tcacaggcga agatgtcgtt gcccaaaaaa cgccgtgcag ttttgatgtc
     8101 tcggtgtggg agtttttctg gccgtttatt gccggggcta aactggtgat ggctgaaccg
     8161 gaagcgcacc gcgacccgct cgctatgcag caattctttg ccgaatatgg cgtaacgacc
     8221 acgcactttg tgccgtcgat gctggcagca tttgttgcct cgctgactcc gcaaaccgct
     8281 cgccagagtt gcgtgacgtt gaaacaggtt ttctgtagtg gtgaggcctt accggctgat
     8341 ttatgccgcg aatggcaaca gttaaccggc gcgccgttgc ataacctgta tggcccgacg
     8401 gaagcggcgg tagatgtcag ctggtatccg gcttttggcg aggaactggc acaggtgcgc
     8461 ggcagcagtg tgccgattgg ttatccggta tggaatacgg gtctgcgtat tcttgatgcg
     8521 atgatgcatc cggtgccgcc gggtgtggcg ggtgatctct atctcaccgg tattcaactg
     8581 gcgcaggggt atcttggacg acccgatctg accgccagcc gctttattgc cgatcctttt
     8641 gctcctggtg aacggatgta tcgtaccgga gacgttgccc gctggctgga taacggcgcg
     8701 gtggagtacc tcgggcgcag tgacgatcag ctaaaaattc gcgggcagcg tatcgaactg
     8761 ggcgaaatcg atcgcgtgat gcaggcgctg ccggatgtca aacaagccgt tacccacgcc
     8821 tgtgtgatta accaggcggc agccactggt ggtgatgcgc gtcagttggt gggctatctg
     8881 gtatcgcaat cgggcctgcc gttggatacc agcgcattgc aggcgcagct tcgcgaaaca
     8941 ttgccgccgc atatggtgcc agtcgttctg ctgcaactgc cgcagttgcc acttagcgcc
     9001 aacggcaagc tggatcgcaa agccttaccg ttgcctgaac tgaaggcaca agcgccaggg
     9061 cgtgcgccga aagcgggcag tgaaacgatt atcgccgcgg cattcgcgtc gttgctgggt
     9121 tgtgacgtgc aggatgccga tgctgatttc tttgcgctgg gcggtcattc gctactggca
     9181 atgaaactgg cagcgcagtt aagtcgacag tttgctcgtc aggtgacgcc ggggcaggtg
     9241 atggtcgcgt caaccgtcgc caaactggca acgattattg atggtgaaga ggacagctcc
     9301 cggcgcatgg gattcgaaac cattctgccg ttgcgtgaag gtaatggccc gacgctgttt
     9361 tgtttccatc cggcgtccgg ttttgcctgg cagtttagcg tgctctcgcg ttatctcgat
     9421 ccacaatggt cgattattgg cattcagtcg ccgcgccctc atggacccat gcagacagcg
     9481 acaaacctgg atgaagtctg cgaagcgcat ctggcaacgc tacttgagca acaaccgcac
     9541 ggcattgcgg cgcggctgcg tgcccgtggc gaacaggtgg catttcttgg tttgctggat
     9601 acctggccgc cagagacgca aaactggcag gaaaaagagg ctaatggtct ggacccggaa
     9661 gtgctggcgg agattaaccg cgaacgcgag gccttcctgg cggcacagca gggaagtact
     9721 tcaacggagt tgtttaccac cattgaaggc aactacgctg atgctgtgcg cctgctgacg
     9781 actgctcata gcgtaccgtt tgatggtaaa gcgacgctgt ttgttgctga acgcacgctt
     9841 caggaaggta tgagccccga acgcgcctgg tcgccgtgga ttgcggagct ggatatctat
     9901 cgtcaggatt gtgcgcatgt ggatattatc tctccagggg cgtttgtaaa aattgggccg
     9961 attattcgcg caacgctaaa caggtaaatt aatattatct ataaaccca
//
LOCUS       DBBBSB010000139         9936 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.139, whole genome shotgun sequence.
ACCESSION   DBBBSB010000139 DBBBSB010000000
VERSION     DBBBSB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.139"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACIUS0_003514"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="ACIUS0_003514"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGB0221110.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACIUS0_003515"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="ACIUS0_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGB0221111.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACIUS0_003516"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="ACIUS0_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGB0221112.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="ACIUS0_003517"
     CDS             complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="ACIUS0_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGB0221113.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="ACIUS0_003518"
     CDS             complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="ACIUS0_003518"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGB0221114.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="ACIUS0_003519"
     CDS             complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="ACIUS0_003519"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGB0221115.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="ACIUS0_003520"
     CDS             complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="ACIUS0_003520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGB0221116.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="ACIUS0_003521"
     CDS             complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="ACIUS0_003521"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGB0221117.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8989..>9936)
                     /gene="lpfD-O113"
                     /locus_tag="ACIUS0_003522"
     CDS             complement(8989..>9936)
                     /gene="lpfD-O113"
                     /locus_tag="ACIUS0_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGB0221118.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2562 a         2586 c         2295 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
     3961 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
     4021 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
     4081 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
     4141 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
     4201 catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata aactcacaaa
     4261 tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc gcggtaagta
     4321 cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
     4381 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
     4441 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
     4501 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
     4561 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
     4621 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
     4681 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
     4741 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
     4801 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
     4861 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
     4921 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
     4981 aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag cctgctgcat
     5041 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
     5101 acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
     5161 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
     5221 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
     5281 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaggc
     5341 gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca tgcccacttt
     5401 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
     5461 ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
     5521 cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt tggcgatatc
     5581 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
     5641 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtacctgat
     5701 gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg catccggaaa
     5761 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga
     5821 cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc gcaaacgggc
     5881 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
     5941 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
     6001 caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc gtaatcgcgg
     6061 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
     6121 ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac agcgccagcg
     6181 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
     6241 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
     6301 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
     6361 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
     6421 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
     6481 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
     6541 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
     6601 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
     6661 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
     6721 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
     6781 cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca tgtacagcga
     6841 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
     6901 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
     6961 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
     7021 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
     7081 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
     7141 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
     7201 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
     7261 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
     7321 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
     7381 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
     7441 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
     7501 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
     7561 aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag ccataagtta
     7621 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
     7681 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
     7741 ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta atccaggtcg
     7801 ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac ctctgtgcct
     7861 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
     7921 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
     7981 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
     8041 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
     8101 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
     8161 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
     8221 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
     8281 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
     8341 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
     8401 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
     8461 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
     8521 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
     8581 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
     8641 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat cgataaggtc
     8701 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
     8761 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
     8821 ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac tgattttaaa
     8881 tgtgaagatg aaaattccac atgccatctt tttgtcatga tgcatttaaa agtgataaag
     8941 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
     9001 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag
     9061 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
     9121 atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
     9181 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
     9241 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
     9301 aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa
     9361 atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat ttatttcgca
     9421 actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct gtgagtaatc
     9481 caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt caggaataat
     9541 caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag ccccactgga
     9601 tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg ggttgttagc
     9661 atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag caatcccaac
     9721 atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag cataagttaa
     9781 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt taccagtatc
     9841 acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa atgtcgttcc
     9901 aaccttattt tcgttcgcgg taataagata tcccga
//
LOCUS       DBBBSB010000140         9914 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.141, whole genome shotgun sequence.
ACCESSION   DBBBSB010000140 DBBBSB010000000
VERSION     DBBBSB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.141"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACIUS0_003523"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACIUS0_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGB0221119.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACIUS0_003524"
     CDS             213..401
                     /locus_tag="ACIUS0_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGB0221120.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACIUS0_003525"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACIUS0_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGB0221121.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACIUS0_003526"
     CDS             596..769
                     /locus_tag="ACIUS0_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGB0221122.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACIUS0_003527"
     CDS             complement(818..1891)
                     /locus_tag="ACIUS0_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGB0221123.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACIUS0_003528"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACIUS0_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACIUS0_003529"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACIUS0_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGB0221124.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACIUS0_003530"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACIUS0_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGB0221125.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACIUS0_003531"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACIUS0_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGB0221126.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..9914
                     /gene="torA"
                     /locus_tag="ACIUS0_003532"
                     /pseudo
     CDS             7739..9914
                     /gene="torA"
                     /locus_tag="ACIUS0_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
BASE COUNT         2398 a         2547 c         2612 g         2357 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagtttatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgc
//
LOCUS       DBBBSB010000141         9857 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.142, whole genome shotgun sequence.
ACCESSION   DBBBSB010000141 DBBBSB010000000
VERSION     DBBBSB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.142"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            383..1501
                     /locus_tag="ACIUS0_003533"
     CDS             383..1501
                     /locus_tag="ACIUS0_003533"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGB0221127.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACIUS0_003534"
     CDS             1567..1815
                     /locus_tag="ACIUS0_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGB0221128.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACIUS0_003535"
     CDS             1880..2248
                     /locus_tag="ACIUS0_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGB0221129.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACIUS0_003536"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACIUS0_003536"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGB0221130.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACIUS0_003537"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACIUS0_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGB0221131.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACIUS0_003538"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACIUS0_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGB0221132.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACIUS0_003539"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACIUS0_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGB0221133.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACIUS0_003540"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACIUS0_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2417 a         2724 c         2385 g         2331 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccac ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccagtatc acttatttaa gtgatagtct taatactagt ttttagactt
     9841 gtcattggag aacagat
//
LOCUS       DBBBSB010000142         9850 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.143, whole genome shotgun sequence.
ACCESSION   DBBBSB010000142 DBBBSB010000000
VERSION     DBBBSB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.143"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..630)
                     /gene="intI1"
                     /locus_tag="ACIUS0_003541"
     CDS             complement(<1..630)
                     /gene="intI1"
                     /locus_tag="ACIUS0_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HGB0221134.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGV"
     gene            788..1261
                     /gene="dfrA17"
                     /locus_tag="ACIUS0_003542"
     CDS             788..1261
                     /gene="dfrA17"
                     /locus_tag="ACIUS0_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HGB0221135.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            1392..2180
                     /locus_tag="ACIUS0_003543"
     CDS             1392..2180
                     /locus_tag="ACIUS0_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HGB0221136.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            2386..2733
                     /locus_tag="ACIUS0_003544"
     CDS             2386..2733
                     /locus_tag="ACIUS0_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HGB0221137.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            2727..3566
                     /gene="sul1"
                     /locus_tag="ACIUS0_003545"
     CDS             2727..3566
                     /gene="sul1"
                     /locus_tag="ACIUS0_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HGB0221138.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            3694..3897
                     /locus_tag="ACIUS0_003546"
     CDS             3694..3897
                     /locus_tag="ACIUS0_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221139.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            4053..5258
                     /gene="chrA"
                     /locus_tag="ACIUS0_003547"
     CDS             4053..5258
                     /gene="chrA"
                     /locus_tag="ACIUS0_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HGB0221140.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            5269..5574
                     /locus_tag="ACIUS0_003548"
     CDS             5269..5574
                     /locus_tag="ACIUS0_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGB0221141.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            5801..6565
                     /locus_tag="ACIUS0_003549"
     CDS             5801..6565
                     /locus_tag="ACIUS0_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HGB0221142.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(7058..7642)
                     /gene="mphR(A)"
                     /locus_tag="ACIUS0_003550"
     CDS             complement(7058..7642)
                     /gene="mphR(A)"
                     /locus_tag="ACIUS0_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HGB0221143.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(7642..8880)
                     /gene="mrx(A)"
                     /locus_tag="ACIUS0_003551"
                     /gene_synonym="mrx"
     CDS             complement(7642..8880)
                     /gene="mrx(A)"
                     /locus_tag="ACIUS0_003551"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HGB0221144.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(8877..9782)
                     /locus_tag="ACIUS0_003552"
     CDS             complement(8877..9782)
                     /locus_tag="ACIUS0_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HGB0221145.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2046 a         2843 c         2900 g         2061 t
ORIGIN      
        1 aacgccgctg cggccctcgg cctggtcctt cagccaccat gcccgtgcac gcgacagctg
       61 ctcgcgcagg ctgggtgcca agctctcggg taacatcaag gcccgatcct tggagccctt
      121 gccctcccgc acgatgatcg tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa
      181 accctcactg atccgcatgc ccgttccata cagaagctgg gcgaacaaac gatgctcgcc
      241 ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg
      301 cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc
      361 gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc
      421 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
      481 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
      541 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      601 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
      661 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
      721 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag ccattaaggg
      781 agttaaattg aaaatatcat tgatttctgc agtgtcagaa aatggcgtaa tcggtagtgg
      841 tcctgatatc ccgtggtcag taaaaggtga gcaactactc tttaaagcgc tcacatataa
      901 tcaatggctc cttgtcggaa gaaaaacatt tgactctatg ggtgttcttc caaatcgcaa
      961 atatgcagta gtgtcaaaga acggaatttc aagctcaaat gaaaacgtcc tagtttttcc
     1021 ttcaatagaa aatgctttga aagagctatc aaaagttaca gatcatgtat atgtctctgg
     1081 cgggggtcaa atctataata gccttattga aaaagcagat ataattcatt tgtctactgt
     1141 tcacgttgaa gtcgaaggtg atatcaaatt ccctataatg cctgagaatt tcaatttggt
     1201 ttttgaacag ttttttatgt ctaatataaa ttatacatac cagatttgga aaaaaggcta
     1261 acaatgcgtt gcagcaccag tcgcttcgct ccttggacag cttttaagtc gcgtctttgt
     1321 ggttttgctg cgcaaaagta ttccacaaag ccgcaactta aaagctgccg ctgaacttaa
     1381 cgttaggcat catgggtgaa tttttccctg cacaagtttt caagcagctg tcccacgctc
     1441 gcgcggtgat cgagcgccat ctggctgcga cactggacac aatccacctg ttcggatctg
     1501 cgatcgatgg agggctgaag ccggacagcg acatagactt gctcgtgacc gtcagcgccg
     1561 cacctaacga ttcgctccgg caggcgctaa tgctcgattt gctgaaagtc tcatcaccgc
     1621 caggcgatgg cggaacatgg cgaccgctgg agctaactgt tgtcgctcga agcgaagtag
     1681 tgccttggcg ctatccggcg cggcgtgagc ttcagttcgg tgagtggctc cgccacgaca
     1741 tcctttccgg aacgttcgag cctgccgttc tggatcacga tcttgcgatt ttgctgacca
     1801 aggcgaggca acacagcctt gcgcttctag gcccatccgc agccacgttt ttcgagccgg
     1861 tgccgaagga gcatttctcc aaggcgcttt tcgacactat tgcccagtgg aatgcagagt
     1921 cggattggaa gggtgacgag cggaacgtcg ttcttgctct tgctcgcatt tggtacagcg
     1981 cttcaactgg tctcattgct cctaaggacg ttgctgccgc atgggtatcg gagcgtttgc
     2041 ctgccgagca tcggcccctc atctgcaagg cacgcgcggc gtacctgggt agcgaggacg
     2101 acgacctagc aatgcgcgtc gaagagacgg ccgcgttcgt tcgatatgcc aaagcaacga
     2161 ttgagagaat cttgcgttga gcggcatgtg cgaaaagtgc atcgacccgc gccgagggca
     2221 tctgatgcct aactcggcgt tcaagcggac gggctgcgcc cgccgctcaa ctatgcgtta
     2281 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     2341 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt
     2401 tttcttgtta tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag
     2461 ggctttacta agcttgcccc ttccgccgtt gtcataatcg gttatggcat cgcattttat
     2521 tttctttctc tggttctgaa atccatccct gtcggtgttg cttatgcagt ctggtcggga
     2581 ctcggcgtcg tcataattac agccattgcc tggttgcttc atgggcaaaa gcttgatgcg
     2641 tggggctttg taggtatggg gctcataatt gctgcctttt tgctcgcccg atccccatcg
     2701 tggaagtcgc tgcggaggcc gacgccatgg tgacggtgtt cggcattctg aatctcaccg
     2761 aggactcctt cttcgatgag agccggcggc tagaccccgc cggcgctgtc accgcggcga
     2821 tcgaaatgct gcgagtcgga tcagacgtcg tggatgtcgg accggccgcc agccatccgg
     2881 acgcgaggcc tgtatcgccg gccgatgaga tcagacgtat tgcgccgctc ttagacgccc
     2941 tgtccgatca gatgcaccgt gtttcaatcg acagcttcca accggaaacc cagcgctatg
     3001 cgctcaagcg cggcgtgggc tacctgaacg atatccaagg atttcctgac cctgcgctct
     3061 atcccgatat tgctgaggcg gactgcaggc tggtggttat gcactcagcg cagcgggatg
     3121 gcatcgccac ccgcaccggt caccttcgac ccgaagacgc gctcgacgag attgtgcggt
     3181 tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt cgctgccgac cggctcatcc
     3241 tcgatccggg gatgggattt ttcttgagcc ccgcaccgga aacatcgctg cacgtgctgt
     3301 cgaaccttca aaagctgaag tcggcgttgg ggcttccgct attggtctcg gtgtcgcgga
     3361 aatccttctt gggcgccacc gttggccttc ctgtaaagga tctgggtcca gcgagccttg
     3421 cggcggaact tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac gcgcctggag
     3481 atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt cgcgacgcca
     3541 gagaccgagg gttagatcat gcctagcatt caccttccgg ccgcccgcta gcggaccctg
     3601 gtcaggttcc gcgaaggtgg gcgcagacat gctgggctcg tcaggatcaa actgcactat
     3661 gaggcggcgg ttcataccgc gccaggggag cgaatggaca gcgaggagcc tccgaacgtt
     3721 cgggtcgcct gctcgggtga tatcgacgag gttgtgcggc tgatgcacga cgctgcggcg
     3781 tggatgtccg ccaagggaac gcccgcctgg gacgtcgcgc ggatcgaccg gacattcgcg
     3841 gagaccttcg tcctgagatc cgagctccta gggatcgcct cagaaaacgg aaaataaagc
     3901 acgctaagcc gtaagtaagc gtgctcctgt gaaagccaca gctaaaactg cgtagtacac
     3961 atagaggtat ttttttctag atttatatcg aatttcgtta tcgaatttcg cttattattg
     4021 atcaactcgt tgcagatcat gaaggcgaaa gtatgacgaa taaccctacc gatgacagca
     4081 gaccatggtc ggtctttctt atttttctgc ggcttggatt gacatctttt ggcggcccca
     4141 ttgcgcactt gggctacttc cgcgccgaat ttgtcacacg gcggcgctgg ctctccgaac
     4201 ggagctatgc tgacttggtc gcgctttgtc agttcttgcc agggcctgca agcagccagg
     4261 tcggcatagc ggtaggactg tctcgggctg gatacagcgg ggcgctggct gcttgggctg
     4321 gcttcacgct gccgtctgcc atagccttga tcctttttgc gctcggcatc tccagctatg
     4381 gcgattacgt ctcgcagggc gcgttgcatg gcttaaaagt ggtggctgtg gccgtggtcg
     4441 ctcaagcagt atggggcatg gcgcgtaacc tatgcacgga tgggctgcga gtcaccatca
     4501 tggcaattgc tacctgcgtc gttttacttg tgccgtccgc gtggggacag gttggcgtga
     4561 ttgctatcgc aggcatcgca ggccggttat tgttcaagcc agcgaaagtt gttgagcatg
     4621 accccctacc tatcacggtc agtcaccggg ccggcgtgct ttggctctcg ctgttctttg
     4681 tcttgctgat tggcctgccg gtgttggccg aactgatgcc aagtcaaacc atggcaatgg
     4741 tggattcctt ctatcgtgtc ggatcactgg tgttcggcgg tggtcacgtt gtgctgccat
     4801 tactgcaagc cgaagtggtg ccctccggct gggtcaacaa tgaatccttt ctcgcggggt
     4861 acggggcagc tcaagcggtg cccggccctt tgttcacgtt cgccgcgttt cttggtgcct
     4921 cgatgaacac cgccccgtcg ggctggatcg gcggcattgt gtgtctgctg gctatcttcg
     4981 cgccctcgtt cttgctggtc gtcggatcaa tgccattttg ggagcgtttg cgccgcaata
     5041 caggcatcca agctgcgctg gccgggatca atgccgctgt agtcggcttg ctgctggccg
     5101 cgctgtatca gcctgtatgg actagcgcca tctttcagcc gcaagacttc ggcttggcat
     5161 tagttgccct tgtcgcactt atgttctgga agctcccgcc gtggctggtt gtcctcggta
     5221 gcggtgcagc tgggtggctg ttgagcgtcg ctctgtgagg cacaaaaaat gactgacaaa
     5281 gacctctacg gcggtttgat ccgcctgcac atccttcacc atgcagccga ggaacctgtc
     5341 tttgggctgg ggatcatcga agagctacgc cgacacggct acgagatgag cgctggcacc
     5401 gtgtacccga tgctgcacgg cctggaaaaa aaaggctatc tgacctcacg ccacgaacgc
     5461 accgggcgac gtgaacggcg tgtttatgac atcactgaac aaggcagaac tgcacttgct
     5521 gatgcgaaaa caaaggtcaa ggagctgttc ggagagttgg tagaaggtgg ttgagttttt
     5581 gcttctatgt caggttgagc tataccctaa ctggattgtc attttcagaa gacgactgca
     5641 ccagttgatt gggcgtaatg gctgttgtgc agccagctcc tgacagttca atatcagaag
     5701 tgatctgcac caatctcgac tatgctcaat actcgtgtgg gctctgttgc aaaaatcgtg
     5761 aagcttgagc atgcttggcg gagattggac ggacggaacg atgacggatt tcaagtggcg
     5821 ccatttccag ggtgatgtga tcctgtgggc ggtgcgctgg tattgtcgct atccgatcag
     5881 ctatcgcgac cttgaggaaa tgctggcgga acgcggcatt tcggtcgacc atacgacgat
     5941 ctatcgctgg gtccagtgct acgccccgga gatggagaag cggctgcgct ggttctggcg
     6001 gcgtggcttt gatccgagct ggcgcctgga tgaaacctac gtcaaggtgc ggggcaagtg
     6061 gacctacctg taccgggcag tcgacaagcg gggcgacacg atcgatttct acctgtcgcc
     6121 gacccgcagc gccaaggcag cgaagcggtt cctgggcaag gccctgcgag gcctgaagca
     6181 ctgggaaaag cctgccacgc tcaataccga caaagcgccg agctatggtg cagcgatcac
     6241 cgaattgaag cgcgaaggaa agctggaccg ggagacggcc caccggcagg tgaagtatct
     6301 caataacgtg atcgaggccg atcacggaaa gctcaagata ctgatcaagc cggtgcgcgg
     6361 tttcaaatcg atccccacgg cctatgccac gatcaaggga ttcgaagtca tgcgagccct
     6421 gcgcaaagga caggctcgcc cctggtgcct gcagcccggc atcaggggcg aggtgcgcct
     6481 tgtggagaga gcttttggca ttgggccctc ggcgctgacg gaggccatgg gcatgctcaa
     6541 ccaccatttc gcagcagccg cctgatcggc gcagagcgac agcctacctc tgactgccgc
     6601 caatctttgc aacagagccc gccgtgctag tctgctcggt gatggtggag tgaagccaac
     6661 ccgcaatcgg gttatgaatc tgcatcgcga ttcgcaatca gctgtctctt gagcatgtcg
     6721 aactcctgcg atgttatctc gccgtgctcg tgctgctaca ccaacttgtc caactcatcc
     6781 gccacaccgc tccccaccac ctgcgatggc caggttgttt gctgggcgca ttggcggagt
     6841 atcgtctcga cccgggacac ccacaccggc acaaccttac gggagtgatt cactgtcaaa
     6901 gaatcggccc ggtgctctga cgcaagtatc gggatggtca ccatttgtaa gccgtagacc
     6961 tgagtggtga tcaagacttc gataccaccg accgtaccgg tactaatcga cgacggtcgt
     7021 gttcgtcgcc tgccgcaggg actctgcaca cctccgttta cgcatgtgcc tggaggagtt
     7081 ggaaatcgtc gtgttcggga aacattaaac acaggatggc agcgatctga gccagcacat
     7141 gatcagctag ctcaccatcc ggatcgacgg cccactgcat cgtcgcgcca gcgatgaccg
     7201 agtgcaggag caactcagct gccgcaggag cacctggggg cagtcgcttg cggatcccct
     7261 ccaccaccgc gcggttccgc tggatcgcaa gcgtgcgtag ctccggcacc tggagctcgt
     7321 accaggagat gagatagttc accgagaagt cgttgcgagt gttcatgctc cgaacgagca
     7381 cctgcaaaaa ttcccagagc ccttgcggcc ctgcgcctat cggtatcgca ttcaggtaat
     7441 gccgcacctg ctcgacgccg cgctccatca tcctcaccag cagcgtatcg cggttggtga
     7501 agcgctggat taacgctgcg cgggagagcc ccacctcctt tgctactccg ctgagcgtga
     7561 actctatggg accgcaacgc ttcagcacta cggtggcggc ctcgagtacc tcgtcatcgg
     7621 acttgagctt ggggcggggc atcagtgttc accttctgta tgggttgggg cggaggctgt
     7681 ggctgccgcc gccattgtag caaattgaag acggagcgag agtagagcca cgagccccgc
     7741 aaacacggcc gatacaacga ggccagggag cggaccagca aggtcgacaa acgcgccggc
     7801 cgcaagcata accatgggcg aggctgacag catcaccgcc gagaccgtgc cgagtacccg
     7861 gccgagaagt tctggcggcg tgcggttgta gatggcagcg ttgagaatgg gagagactga
     7921 gccggtcagc agtcccacga gcgcgcccaa caacatcagc accggcacgc ctggcaactg
     7981 tgaaagcaga agcgagccca ccgcagagcc acaaaatgcc accgccagcc agttctgcgc
     8041 tgatatccgg gcgccgaccg acgcatgaat ggcaatgcca aggagaccac cagcccccat
     8101 cattgaggag aacagcccga gctctgctac ttggcgtcct gcatctacaa acagcgcagg
     8161 catgatgacg ctgccgttgg cgccaacgat gcccacgaag atcatcacta taccaaagag
     8221 agggcgcagc aggggttcgc tccagagaaa agcgacgccg gcgcgcatgg agagagtcgc
     8281 cgtcgtggtc atcgtccgag cggcacgcgc gggaagcacc cacgcgccga gcagacctgc
     8341 aaggacggag cagaacgccg tcagcccgag cgttggcgca gcgccaagca ggccgattgc
     8401 ggccccccca agggccgggc cacctagaat cgcgacgttc ccgatcaccg ctttcagtga
     8461 cgagacgcgc tcaacggaga gcccggcgac gtggccgagt ttgggcagct cactgtcctg
     8521 cgcggccata ccgggtgcgt cgaacgcggc accgagcacc acgcaagcga tcagcccagt
     8581 gttcgagagg gcgccaacgg catcgagcag tgggatgctc gccatggcca cgccgcccac
     8641 cacacccgag atcaatgcga cgggcgcgcg cccgaaccga tcgacgaggc caccaccaac
     8701 ccacgcgccg atgatggtcg cgatgacgct gctagcggcc gtggcgcccg cccaggccgc
     8761 gctctttgta tgagacagga cgaaccatgg aagcgcgagg gccgccaccg cgttgccgat
     8821 ccggaagaga aaggtcgccg cgaacagcgt cgcgagcggg ctatatcgac gttcgctcat
     8881 tccgctgcgg cgagctgcgc cttcgccgca gcgaggtact cttcgttacc cgagtcgagg
     8941 gcgaagagtg cgtaggtgac cgccccgaac gcaaggcgct ccgcgatgtg gtgggcgagc
     9001 cgcggccaca cccggccacc ggccgcttca tacgtgagga ggagcttcgc gagcccctct
     9061 tcaccaaaga ccataaggtg cgcggccatg tcgatggcag ggtcatcaac gcgggcctcg
     9121 ctccagtcga tcatcccgct gacgcgctcc gtgttgtcga tgagcacatg gcccacgtag
     9181 agatcgccat gcaccaccac ggagaaatct ggccacgacg aatcgtcgtc gagccagcgc
     9241 tgccaccggt ggaggcgctt gtcgttcacc acgaactcgc gtcggacgcg gtcaacgtcg
     9301 tcggccacct tctgacgggc ctgcgtcggt gtacggatga gcatccccgc atccacggcg
     9361 gcggaaatgg ggacggcatg cagggcggcg agcgcggtcg cgaagctctc cgcgaagacc
     9421 tccgagtcct gcggcacgac ccagtcgggc gtggacgaac caggctggat gaccatcgca
     9481 gtcgagtctt cgagcatggg ataggcaacg agctcggcgt tggccacgcg ccagtccggc
     9541 accgcgaacg gcaggcgatt cttgagcatt gccagcaccc gcgcctctgg ttcgaccttc
     9601 gcgcttacct cggctcggcg cgggatgcgc agcacccacc gacgtccatc gtcgacggtg
     9661 gcgatcacga tcctatagtc gagcccaagc tcattgacag tcagcgggcc atggagcttg
     9721 agcccatgtc gggctgcaag tgcgtacagt tgggaggtat cggcggtcgt gactacggtc
     9781 atgattcact cctgagggct tgacgggttt agccacctaa atgtaacagt cacgtcggtt
     9841 atattcaatc
//
LOCUS       DBBBSB010000143         9774 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.144, whole genome shotgun sequence.
ACCESSION   DBBBSB010000143 DBBBSB010000000
VERSION     DBBBSB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.144"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACIUS0_003553"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACIUS0_003553"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGB0221146.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACIUS0_003554"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACIUS0_003554"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGB0221147.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACIUS0_003555"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACIUS0_003555"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGB0221148.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACIUS0_003556"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACIUS0_003556"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGB0221149.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACIUS0_003557"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACIUS0_003557"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGB0221150.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACIUS0_003558"
     CDS             complement(6031..6360)
                     /locus_tag="ACIUS0_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGB0221151.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACIUS0_003559"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACIUS0_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGB0221152.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACIUS0_003560"
     CDS             7998..8171
                     /locus_tag="ACIUS0_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGB0221153.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACIUS0_003561"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACIUS0_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGB0221154.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DBBBSB010000144         9681 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.146, whole genome shotgun sequence.
ACCESSION   DBBBSB010000144 DBBBSB010000000
VERSION     DBBBSB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.146"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(44..214)
                     /locus_tag="ACIUS0_003562"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACIUS0_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACIUS0_003563"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACIUS0_003563"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGB0221155.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACIUS0_003564"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACIUS0_003564"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGB0221156.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACIUS0_003565"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACIUS0_003565"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGB0221157.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACIUS0_003566"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACIUS0_003566"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGB0221158.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACIUS0_003567"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACIUS0_003567"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGB0221159.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="ACIUS0_003568"
     CDS             5109..6242
                     /locus_tag="ACIUS0_003568"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGB0221160.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="ACIUS0_003569"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="ACIUS0_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGB0221161.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACIUS0_003570"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="ACIUS0_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGB0221162.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACIUS0_003571"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="ACIUS0_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGB0221163.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACIUS0_003572"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="ACIUS0_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGB0221164.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACIUS0_003573"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="ACIUS0_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGB0221165.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="ACIUS0_003574"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="ACIUS0_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGB0221166.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DBBBSB010000145         9595 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.147, whole genome shotgun sequence.
ACCESSION   DBBBSB010000145 DBBBSB010000000
VERSION     DBBBSB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.147"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACIUS0_003575"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACIUS0_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGB0221167.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACIUS0_003576"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACIUS0_003576"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGB0221168.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACIUS0_003577"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACIUS0_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGB0221169.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="ACIUS0_003578"
     CDS             complement(2404..2607)
                     /locus_tag="ACIUS0_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221170.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="ACIUS0_003579"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="ACIUS0_003579"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGB0221171.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="ACIUS0_003580"
     CDS             complement(3803..4015)
                     /locus_tag="ACIUS0_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGB0221172.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="ACIUS0_003581"
     CDS             4275..4583
                     /locus_tag="ACIUS0_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGB0221173.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="ACIUS0_003582"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="ACIUS0_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGB0221174.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="ACIUS0_003583"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="ACIUS0_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGB0221175.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="ACIUS0_003584"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="ACIUS0_003584"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGB0221176.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="ACIUS0_003585"
     CDS             complement(8479..8982)
                     /locus_tag="ACIUS0_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221177.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9595)
                     /locus_tag="ACIUS0_003586"
     CDS             complement(9070..>9595)
                     /locus_tag="ACIUS0_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGB0221178.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DBBBSB010000146         9537 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.148, whole genome shotgun sequence.
ACCESSION   DBBBSB010000146 DBBBSB010000000
VERSION     DBBBSB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.148"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACIUS0_003587"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACIUS0_003587"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGB0221179.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACIUS0_003588"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACIUS0_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGB0221180.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACIUS0_003589"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACIUS0_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACIUS0_003590"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACIUS0_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGB0221181.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACIUS0_003591"
     tRNA            4965..5038
                     /locus_tag="ACIUS0_003591"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACIUS0_003592"
     CDS             5173..6783
                     /locus_tag="ACIUS0_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221182.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACIUS0_003593"
     CDS             6815..7234
                     /locus_tag="ACIUS0_003593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221183.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACIUS0_003594"
     CDS             8353..9099
                     /locus_tag="ACIUS0_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGB0221184.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACIUS0_003595"
     CDS             9397..>9537
                     /locus_tag="ACIUS0_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221185.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DBBBSB010000147         9496 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.149, whole genome shotgun sequence.
ACCESSION   DBBBSB010000147 DBBBSB010000000
VERSION     DBBBSB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.149"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="ACIUS0_003596"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="ACIUS0_003596"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGB0221186.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="ACIUS0_003597"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="ACIUS0_003597"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGB0221187.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="ACIUS0_003598"
     CDS             3116..3400
                     /locus_tag="ACIUS0_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGB0221188.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACIUS0_003599"
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="ACIUS0_003599"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGB0221189.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACIUS0_003600"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="ACIUS0_003600"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGB0221190.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACIUS0_003601"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="ACIUS0_003601"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGB0221191.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="ACIUS0_003602"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="ACIUS0_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGB0221192.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2277 a         2500 c         2394 g         2325 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttgttacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gaggaagagt gcatcgtggt cgccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatggatgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cctataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacca gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaaggg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg taactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaat gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcaccgc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tatttcgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tatctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttata ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcacta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcattt cgacttcgat cacatatcga taaataatat tattctattt ttctcattgt
     9481 ttttaatcac cattct
//
LOCUS       DBBBSB010000148         9494 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.150, whole genome shotgun sequence.
ACCESSION   DBBBSB010000148 DBBBSB010000000
VERSION     DBBBSB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.150"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..190)
                     /locus_tag="ACIUS0_003603"
     CDS             complement(<1..190)
                     /locus_tag="ACIUS0_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGB0221193.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACIUS0_003604"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACIUS0_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGB0221194.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACIUS0_003605"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACIUS0_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGB0221195.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACIUS0_003606"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACIUS0_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGB0221196.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACIUS0_003607"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACIUS0_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACIUS0_003608"
     CDS             complement(3262..3381)
                     /locus_tag="ACIUS0_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGB0221197.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACIUS0_003609"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACIUS0_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGB0221198.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACIUS0_003610"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACIUS0_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGB0221199.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACIUS0_003611"
     CDS             3803..4147
                     /locus_tag="ACIUS0_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGB0221200.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACIUS0_003612"
     CDS             4151..4486
                     /locus_tag="ACIUS0_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGB0221201.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACIUS0_003613"
     CDS             complement(4543..6864)
                     /locus_tag="ACIUS0_003613"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGB0221202.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACIUS0_003614"
     CDS             complement(6981..7190)
                     /locus_tag="ACIUS0_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221203.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACIUS0_003615"
                     /pseudo
     CDS             complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACIUS0_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7941..9464)
                     /locus_tag="ACIUS0_003616"
     CDS             complement(7941..9464)
                     /locus_tag="ACIUS0_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGB0221204.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2660 a         2207 c         2079 g         2548 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaattcaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DBBBSB010000149         9448 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.151, whole genome shotgun sequence.
ACCESSION   DBBBSB010000149 DBBBSB010000000
VERSION     DBBBSB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.151"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACIUS0_003617"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACIUS0_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGB0221205.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACIUS0_003618"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACIUS0_003618"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGB0221206.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACIUS0_003619"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACIUS0_003619"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGB0221207.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACIUS0_003620"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACIUS0_003620"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGB0221208.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACIUS0_003621"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACIUS0_003621"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGB0221209.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACIUS0_003622"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACIUS0_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGB0221210.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACIUS0_003623"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="ACIUS0_003623"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGB0221211.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACIUS0_003624"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="ACIUS0_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGB0221212.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACIUS0_003625"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="ACIUS0_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGB0221213.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACIUS0_003626"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="ACIUS0_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGB0221214.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..>9448)
                     /locus_tag="ACIUS0_003627"
     CDS             complement(9181..>9448)
                     /locus_tag="ACIUS0_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGB0221215.1"
                     /translation="AAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKA
                     RKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         2400 a         2371 c         2409 g         2268 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcggct
//
LOCUS       DBBBSB010000150         9440 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.152, whole genome shotgun sequence.
ACCESSION   DBBBSB010000150 DBBBSB010000000
VERSION     DBBBSB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.152"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1154
                     /locus_tag="ACIUS0_003628"
     CDS             <1..1154
                     /locus_tag="ACIUS0_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221216.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACIUS0_003629"
     CDS             1833..2051
                     /locus_tag="ACIUS0_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGB0221217.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACIUS0_003630"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACIUS0_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGB0221218.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACIUS0_003631"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACIUS0_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGB0221219.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACIUS0_003632"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACIUS0_003632"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGB0221220.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACIUS0_003633"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACIUS0_003633"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGB0221221.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACIUS0_003634"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACIUS0_003634"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGB0221222.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACIUS0_003635"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACIUS0_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGB0221223.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DBBBSB010000151         9302 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.153, whole genome shotgun sequence.
ACCESSION   DBBBSB010000151 DBBBSB010000000
VERSION     DBBBSB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.153"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACIUS0_003636"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACIUS0_003636"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGB0221224.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1649..3049
                     /locus_tag="ACIUS0_003637"
     CDS             1649..3049
                     /locus_tag="ACIUS0_003637"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGB0221225.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3419..4243
                     /gene="gadX"
                     /locus_tag="ACIUS0_003638"
     CDS             3419..4243
                     /gene="gadX"
                     /locus_tag="ACIUS0_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGB0221226.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4611..5339
                     /gene="gadW"
                     /locus_tag="ACIUS0_003639"
     CDS             4611..5339
                     /gene="gadW"
                     /locus_tag="ACIUS0_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGB0221227.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5702..8815)
                     /gene="mdtF"
                     /locus_tag="ACIUS0_003640"
                     /pseudo
     CDS             complement(5702..8815)
                     /gene="mdtF"
                     /locus_tag="ACIUS0_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8840..9097)
                     /locus_tag="ACIUS0_003641"
                     /pseudo
     CDS             complement(8840..9097)
                     /locus_tag="ACIUS0_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2436 a         2160 c         2274 g         2432 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agttcgaaat ggaccagaag ctgttaacgg atttccgctc
     1681 agaactactc gattcacgtt ttggcgcaaa ggccatttct actatcgcgg agtcaaaacg
     1741 atttccgctg cacgaaatgc gcgatgatgt cgcattccag attatcaatg atgaattata
     1801 tcttgatggc aacgctcgtc agaacctagc cactttctgc cagacctggg acgacgaaaa
     1861 cgtccataaa ttgatggatt tgtcgatcaa taaaaactgg atcgacaaag aagaatatcc
     1921 gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt gccgatctgt ggcatgcgcc
     1981 tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
     2041 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggcaaacc
     2101 aacggataaa ccaaacctag tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
     2161 ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
     2221 gaaacgcatg attgaagcct gtgacgaaaa caccatcggc gtggtgccga ctttcggcgt
     2281 gacctacacc ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca
     2341 ggccgacacc ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc
     2401 accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag
     2461 tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga
     2521 cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg
     2581 tacttttgcc atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt
     2641 cctgcgcctc ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc
     2701 ttatctggcg gatgaaatcg ccaaacaggg gccgtatgag ttcatctgta cgggtcgccc
     2761 ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac
     2821 cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct
     2881 cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat
     2941 ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca
     3001 cccgaaactg cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt
     3061 tgtaaaaacc gaatgcccag cctttaaaaa aacagctggg cattcggttg cttattaatc
     3121 gcaataatat attggctgtc tattcatcgt gttgataaga tatatgttat atgaatgttt
     3181 attaaatgta gcgattacct ttaatgtcac gaaaagggat tatttgcttg ctattaattt
     3241 cactgcgttc ttaaacacgt ttttgtatta taaatccagt catcctgccc gtagaacatc
     3301 acacattatc atcctgttct cccgcttcgt ttaaatttat ttatcaatca atttgactta
     3361 agagggcggc gtgctacatt aattaacagt aatatgttta tgtaatatta agtcaactat
     3421 gcaatcatta catgggaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
     3481 ggttaatggt gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca
     3541 aattcgagta gataagtgtg ttgaaaattt tgtattagta tcaagggata cgctttcatt
     3601 atttctgccg atgctcaagg aggaggcatt aaatcttcat gcacataaaa aaatttcttc
     3661 attactcgtt catcactgta gcagagatat tcctgttttt caggaagttg cgcaactatc
     3721 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt
     3781 gttatctgtt tttcttgagg atgagcactt tataccgctg cttttgaacg ttttgcaacc
     3841 gaacatgcga acacgagttt gtacggttat caataataat atcgcccatg agtggacact
     3901 agcccgaatc gccagcgagc tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
     3961 agaagagaca tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact
     4021 tattgttata catggctttt caattaagcg agtcgcagtg tcctgtggat atcacagcgt
     4081 gtcgtatttc atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga
     4141 gcgatcggcg cagggattgc cgaaccgtga ctcggcggca agtattgttg cgcaagggaa
     4201 tttttacggc actaaccgtt ctgcggaagg aataagatta tagagtttta ctcagacata
     4261 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
     4321 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
     4381 tttacaggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgcgaaaat
     4441 atattcgtgc tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     4501 aattttggtc ctaccaaatc tggcagtttt tgcgctaaga aacagtctgt catcatttca
     4561 ttagtatact gaaattgaaa taatcgcagt atgaaatata agggataatc atgactcatg
     4621 tctgctcggt gatcctcatt cgtcgttcat tcgatattta tcatgaacag caaaaaatat
     4681 cgctgcataa cgagagtatc ctgctgctgg agaaaaattt ggcagacgat tttgcgtttt
     4741 gttcaccgga tacgcgacga ctggatatcg atgagctgac agtttgccat tacttacaaa
     4801 atattcgtca gctaccacgc aatttagggt tacatagcaa agaccgtctg ttaattaacc
     4861 agtcaccccc catgccgctg gtgacggcga tttttgatag cttcaatgaa tccggggtaa
     4921 attcaccgat actgagcaat atgctctacc tttcctgttt atcgatgttt tctcataaga
     4981 aagaactgat ccccttactt ttcaatagca tcagcactgt ttcaggaaaa gttgaacgcc
     5041 ttattagctt tgatatcgcc aaacgttggt atctgcgcga tatcgcggaa agaatgtata
     5101 ccagcgagag tctcatcaaa aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat
     5161 tactcgcttc caggatgtcg atggccagac gattactcga gttacgtcaa attcctctgc
     5221 atactattgc ggaaaaatgt ggctatagca gtacgtcgta ctttataaac acatttcgac
     5281 aatattatgg tgtaacgcca catcagtttg cgcaacattc gccaggtacc ttttcctgac
     5341 atattttgca tttgaatatt ggtcaggatc tcacacctgc ttcatgtgaa actcttccct
     5401 gatgatttct gccgggctac cggctagttc tctttcgcag tttgccaggc ggtataggta
     5461 tttttaggag gaaaagactc ggcatgtttg ggattattaa gctgacaatt catacgatta
     5521 cgggaatgtg ggtcagtatt gtgttgttca aattgatgac gaatggctgg agtggtttct
     5581 actttcagtg ctgtgtacta agcctggtat tcctgacggt tagctggttg ttgagtggtg
     5641 aatggttagc aggaaagagt aaggctgaac cttcacgttc aaccttactc tcatttacac
     5701 gttacgcttt tttaaagcgg gcaaagagat gttccactac aacgaaaaag accggaacga
     5761 agtaaattgc cagcactgtt gcggcaaaca tcccgcccat cacgccggta cctaccgcgt
     5821 tttgcgcgcc agaaccggca ccatgactga taaccagcgg cagcacgccg agaataaagg
     5881 ccagagaggt catcaggatt gggcgtaaac gcatccgcgc cgcttcgatg attgcctcta
     5941 tcggcgtttt cccttctttc tgcatcatct caacggcaaa ttcgacaatc aggatggcgt
     6001 tcttggcgga aagcccgatg gtggtcagca aaccaacctg gaagtagacg tcattactta
     6061 agccgcgcag atcggtggcc agtaatgcgc caacgacgcc taacggaaca accaacatca
     6121 ccgagaacgg aattgaccag ctctcataga gtgcggcgag ggcgaggaac accacgacca
     6181 gtgaaatggc atacagtgca ggagcctggt ttgaggataa cgcttcctga tacgacagtc
     6241 cggtccatga gtagccgacg cctgccggaa gtttagcgac caggtctgcc ataaatttca
     6301 tagcgtcacc ggtacttttc ccggccgccg cttcacctaa aatctccatt gacgggatgc
     6361 cgttgtagcg ttccaatcgc ggtgaaccat aggtccattc ggtagacgag taggcagaaa
     6421 gcggtgccat cgtgccagag gcgttgcgta cataccattg gttgatgtta tccggcaaca
     6481 tacggaacgg cgtgcctgcc tggacataca cttttttcac ccgcccctgg ttgaggaagt
     6541 cgttcacgta gctgctgccg aaggcggtgg aaattgtctg gttgatatca gacagcgcca
     6601 cacccattgc ctcagctttc gcggcgttga cgttcacttt gaacatcggc gtatcttcca
     6661 ggccgttcgg gcgtaccccg gtgacctggt caggcgattg cgctgccagt gataacagct
     6721 cgtttcgtgc ctaggttagt ttttcgtgcc ccaggttacc gttgtccagc agttccatat
     6781 caaaacctga cgcggtaccc agttcagcca ccgcgggtaa gttgaacggg aagacgacgg
     6841 ctttattgat actgcttaac gcaatcattg cccgctgaat gatcgcggta accgagtttt
     6901 cctcaccgac acgttcagac cacggcttga gactgataaa cgccaggccg ttgttttgcc
     6961 cctggccgct gaagccaaag ccgccaacgg taaacaccga ctgaacatta tctttctctt
     7021 ttgtaagata ataatccgtc acctgttgca gtactttcgt ggtgttaacc atggtggcac
     7081 cggaaggtaa ctgcgcggtg gtcataaata ccccctgatc ctcttctggt aagaaagagg
     7141 tcggtgtacg caggaacagc accgccatcc cggcgcaaat cagcaggtag accaccatgt
     7201 agcgaccggt acaacgcaac agcgagcggg tgctatcggt atagtgctga gttgattttt
     7261 caaacagcgt gttgaagcgt gcgaacaggg cgttaggttt gtgaccgcct tccggcgtgg
     7321 ctttcagaat ggtggcgcac agggcagggg tcaggctcat tgccacaaat actgaaagcc
     7381 gcatggagga gatcagcgtg atggagaact ggcggtagat ctccccggtt gcaccgctca
     7441 taaaggccat cggcataaac actgcagaaa gaacaacggc aatcccgacc agcgcacgtt
     7501 ggatctgccc catcgattta tgcgtcgctt ccttcggcgg tagcttatct tccgcaatga
     7561 cacgctcgac gttctccacc accacgatgg cgtcatccac cagtaaccct atcgccagca
     7621 ccatcccgaa catagtcaac gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg
     7681 tcccgagaat aaccaccggt acggcaattg tcgggatgat ggtggcacgg aaattctgca
     7741 aaaacagata aatgaccagg aagacgagga tgatagcctc aaccagtgtt ttgaaaactt
     7801 cctgaataga aatttcgata aacggcgtgg tgtcgtaagg ataaaccgtc ttcagacttg
     7861 cggggaaata ggctgataag cggttcagct cctctttgac tgcccgcgag gtatccaggg
     7921 cgtttgctcc ggcagccagt ttgatggcga tcccggcaac aggtttgccg ttatagcgcg
     7981 ccacggtgga ataatcttcc gccccaagtt cgacgcgggc gacatcacgc agcagcactt
     8041 gcgaaccatc ttgctgaact ttcaacagga ttttgccaaa ttcttccggc gtttgcagac
     8101 gcgtctgcac aatgatcgag gcgtttagtt gctggtctgc cgcctgtggc atgccaccca
     8161 gttgaccacc agaaatctgg ttgttttgca ccttaatctg ggaaataaca tcggaaggta
     8221 ccaggttata tttattgagt ttttgcgggt ccagccagat acgcatggcg tactcggaac
     8281 caaagagctg tacgctacca acgcccgcgg tacggcttag cgggtctttg atattagacg
     8341 ctacatagtc cgcgatatcg tactggttga ggctgccgtt atcagaaata aacgccgcta
     8401 ccatcaggat attactgctc gacttatcga cgctaatccc ctgttgctgc accgcttcag
     8461 gtaatgaagg catagcgagt tgcagtttat tttgcacctg aacctgtgcg atatcaggag
     8521 atgccccagt ctcgaaggtc agagtgatag aggcattacc cgccgcatca ctggttgaag
     8581 acatgtacat caggccatca agcccattca tattttgctc aatcacctga gtgaccgagt
     8641 cttctaccgt ttgcgcatcg gcacccggat aggtagcgct gacggtaatg gtcggtggcg
     8701 caatctgcgg atactgcgca accggtaagt tcatgatcgc cagaccacct gcaagcatca
     8761 taataatggc aagtacccag gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag
     8821 tccccttaag cctgcaacgt tattgtttcg attcggtgct ggcgttttcc tggctggagg
     8881 aaattgctcg tgctttgata cccggacgaa tgcgttgcaa accggaaacg atcacccgat
     8941 cgccagcctg caagccagag gtgacaaccc actggtcgcc gatggctttg ctggcttcaa
     9001 tttcgcgtag ctgcacgaca tcgtctttat ccagaatgag cgccgttgct ttaccctggg
     9061 cgttgtgggt gacgccttcc tgcggcacca gtaatataaa gcgcgcaaaa ttattcaaga
     9121 tatttttgaa aatcattcaa gataggctat cgcgtgtgat agcaaaaaca ttcaagataa
     9181 aaatgcttgt taattaacaa atgatatctt ctatatattt ttattgttta tagatggcta
     9241 tttcggtaaa aatgcatttt cagaatatca accagcggta aatcgaagta aagggagtga
     9301 at
//
LOCUS       DBBBSB010000152         9154 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.154, whole genome shotgun sequence.
ACCESSION   DBBBSB010000152 DBBBSB010000000
VERSION     DBBBSB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.154"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..924
                     /locus_tag="ACIUS0_003642"
     CDS             <1..924
                     /locus_tag="ACIUS0_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0221228.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="ACIUS0_003643"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="ACIUS0_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGB0221229.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="ACIUS0_003644"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="ACIUS0_003644"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGB0221230.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="ACIUS0_003645"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="ACIUS0_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGB0221231.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="ACIUS0_003646"
     CDS             4447..4806
                     /locus_tag="ACIUS0_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGB0221232.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="ACIUS0_003647"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="ACIUS0_003647"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGB0221233.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="ACIUS0_003648"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="ACIUS0_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGB0221234.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="ACIUS0_003649"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="ACIUS0_003649"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGB0221235.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2091 a         2212 c         2474 g         2377 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgtc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tttacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccatgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaagtc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgctgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctttactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 taaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggtgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatg
//
LOCUS       DBBBSB010000153         9152 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.155, whole genome shotgun sequence.
ACCESSION   DBBBSB010000153 DBBBSB010000000
VERSION     DBBBSB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.155"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACIUS0_003650"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACIUS0_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGB0221236.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACIUS0_003651"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACIUS0_003651"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGB0221237.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACIUS0_003652"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACIUS0_003652"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGB0221238.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACIUS0_003653"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACIUS0_003653"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGB0221239.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACIUS0_003654"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACIUS0_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGB0221240.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACIUS0_003655"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACIUS0_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGB0221241.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACIUS0_003656"
     CDS             6064..6606
                     /locus_tag="ACIUS0_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGB0221242.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACIUS0_003657"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACIUS0_003657"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGB0221243.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACIUS0_003658"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACIUS0_003658"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGB0221244.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACIUS0_003659"
     CDS             8003..8260
                     /locus_tag="ACIUS0_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGB0221245.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACIUS0_003660"
     CDS             complement(8236..8790)
                     /locus_tag="ACIUS0_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGB0221246.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2298 a         2220 c         2280 g         2354 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgcaactgct
     9121 ctttaacaat ttatcagaca atctgtgtgg gc
//
LOCUS       DBBBSB010000154         8767 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.156, whole genome shotgun sequence.
ACCESSION   DBBBSB010000154 DBBBSB010000000
VERSION     DBBBSB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.156"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..439
                     /locus_tag="ACIUS0_003661"
     CDS             <1..439
                     /locus_tag="ACIUS0_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGB0221247.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACIUS0_003662"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACIUS0_003662"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGB0221248.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACIUS0_003663"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACIUS0_003663"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGB0221249.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACIUS0_003664"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACIUS0_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGB0221250.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACIUS0_003665"
     CDS             complement(4441..5148)
                     /locus_tag="ACIUS0_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGB0221251.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACIUS0_003666"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACIUS0_003666"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGB0221252.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACIUS0_003667"
     CDS             complement(6006..6209)
                     /locus_tag="ACIUS0_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGB0221253.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACIUS0_003668"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACIUS0_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8767
                     /locus_tag="ACIUS0_003669"
     CDS             7873..>8767
                     /locus_tag="ACIUS0_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGB0221254.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2036 a         2389 c         2252 g         2090 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
     8761 gaacaat
//
LOCUS       DBBBSB010000155         8697 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.157, whole genome shotgun sequence.
ACCESSION   DBBBSB010000155 DBBBSB010000000
VERSION     DBBBSB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.157"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(42..134)
                     /locus_tag="ACIUS0_003670"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACIUS0_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACIUS0_003671"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="ACIUS0_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGB0221255.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3235)
                     /gene="hemX"
                     /locus_tag="ACIUS0_003672"
     CDS             complement(2012..3235)
                     /gene="hemX"
                     /locus_tag="ACIUS0_003672"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGB0221256.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(3257..3997)
                     /gene="hemD"
                     /locus_tag="ACIUS0_003673"
     CDS             complement(3257..3997)
                     /gene="hemD"
                     /locus_tag="ACIUS0_003673"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGB0221257.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3994..4935)
                     /gene="hemC"
                     /locus_tag="ACIUS0_003674"
     CDS             complement(3994..4935)
                     /gene="hemC"
                     /locus_tag="ACIUS0_003674"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGB0221258.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5322..7868
                     /gene="cyaA"
                     /locus_tag="ACIUS0_003675"
     CDS             5322..7868
                     /gene="cyaA"
                     /locus_tag="ACIUS0_003675"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGB0221259.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7908..8228)
                     /gene="cyaY"
                     /locus_tag="ACIUS0_003676"
     CDS             complement(7908..8228)
                     /gene="cyaY"
                     /locus_tag="ACIUS0_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGB0221260.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8276..>8697)
                     /locus_tag="ACIUS0_003677"
     CDS             complement(8276..>8697)
                     /locus_tag="ACIUS0_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HGB0221261.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1978 a         2243 c         2244 g         2232 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     2101 gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta cgcacgcgag
     2161 tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga
     2221 tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag
     2281 tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga taagtctctt
     2341 cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca
     2401 gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg
     2461 taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga
     2521 tggaactgga aagctcttca ccgtctgaat ccatcggcga accatcgctg tcattatcgg
     2581 ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat
     2641 aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg
     2701 taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg
     2761 cagaggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat
     2821 cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg atcttttgtt
     2881 ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc ttaagctgcg
     2941 cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg gctttttgca
     3001 atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt
     3061 taccccagcc atacaaaccg acacccgccg ccagagcaat agcgatagcc accgcgctga
     3121 gaatcaatgc agtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt
     3181 ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg
     3241 cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg
     3301 cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga
     3361 ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga
     3421 gttgctgcaa catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg gattgccagc
     3481 gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag
     3541 tgacctcagc accgcgcacc gtcagggtat ccccaattag ctcacgaccg ccattgccac
     3601 gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt
     3661 cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta tgtagcgcca
     3721 gtgcggtggt gcgtccaatg gcgaaataag taggtagttg gggccattta cgatcttgct
     3781 gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc
     3841 tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact
     3901 caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt
     3961 ctccagcggg agacgggcgg gtgaccagga tactcatgcc ggggcctctc cgttatagac
     4021 ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat
     4081 ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg
     4141 cgcgccgacc agcgcacata gccagatttc gccatcaata agctcggcgt agctaccaat
     4201 tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag
     4261 tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat catcaaggcg
     4321 gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc
     4381 ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc
     4441 atcgtattcg ccgttatcta gtttgctcag gcgagtgccg acgttgccgc gcagggagcg
     4501 gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc
     4561 gacgatactg cctgctggta acgcatccag actgtcatag ttattggaca caaaggcatc
     4621 gcgaggatct tcacgctcgc aaatagtgac cagccccaga ccttgcggga attcaaccgg
     4681 cacatctttc attgaatgta cggcgatatc agcgcgattc tccaggagcg cgacttccag
     4741 ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc
     4801 gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt
     4861 gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa
     4921 aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg
     4981 ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt
     5041 ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta cagagaataa
     5101 acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat
     5161 tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg
     5221 taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc
     5281 ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga
     5341 ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta
     5401 tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc
     5461 cgcgaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgcctg
     5521 atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg
     5581 atccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg
     5641 tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata
     5701 gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc
     5761 tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat aatgaaagcg
     5821 gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt
     5881 atcgtactgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg
     5941 aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa
     6001 atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac tttggtgcca
     6061 gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg aaaacactgc
     6121 tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac
     6181 agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc atgatgctgg
     6241 agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat ttagtacgtc
     6301 gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc
     6361 gtcgcgcagt gttgagccag ttagtgagcg agtggggttg ggacgaagct cgtctggcaa
     6421 tgctcgataa ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagctgc
     6481 tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta
     6541 gcgtctccgc cagtccgcag gatatcggcg tactgacgcg taaactgtat gccgcgtttg
     6601 aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac
     6661 cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt
     6721 ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt
     6781 acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg cgcacccgtt
     6841 tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg
     6901 tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt
     6961 gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca gctgcgttcc
     7021 gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc
     7081 aaaattgcct ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta
     7141 cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa
     7201 tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc
     7261 gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt
     7321 ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg
     7381 gcttgttctt cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc atcgagtttt
     7441 atggcgctat ttcgcataac aaactgcacg gcttgtcagt gcaggttgaa accaatcacg
     7501 tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag ttctttttcg
     7561 aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc aaccgggttg
     7621 aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc agtcgcttct
     7681 actcgtcatc gcacgaccgc ttcacctacg gctcaagctt catcaacttc aacctgccgc
     7741 agttctatca gattgtgaag gttgatggtc gcgaacaggt gattccgttc cgaacaaaat
     7801 cgattggcaa cctgccgccc gccaatcagg atcacgatac gccgctatta cagcagtatt
     7861 tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg
     7921 actgtttcac ccgcctgttg cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg
     7981 ctgcgatcgc aaatccattc atcgcctttc aggtcaaaat ggtagccgcc ctgtttggtt
     8041 gccagccaca cctggtgcag cggctcctgg cggttaataa tgattttgct gccgttttca
     8101 aaggtaatgg tcagtacgcc gccgttgatt tcgcagtcga tatcgctgtc gccatccctg
     8161 tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg aaattcactg
     8221 tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg
     8281 gcttcaaagt ttgcacctct gctgctgcgt tccggcatga ttcatccgtc accggaataa
     8341 tgatgtctct gttttgaact tcacgtacgt tctcctctct gacacgagtt ttcgccgggc
     8401 agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg cgatcgtcat
     8461 aagcgaagcg atcgttttta gttagcgaac cgttaactgg aataatttgc aagctctttg
     8521 accgtaattg gcggataaag taagcatgct gtttgccgct attaaatgag cgatcaatga
     8581 ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat gtcgataatt
     8641 ttgttacata cagccgatct ttatattgcg caatattgcc agtaatcaga tggaatt
//
LOCUS       DBBBSB010000156         8594 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.158, whole genome shotgun sequence.
ACCESSION   DBBBSB010000156 DBBBSB010000000
VERSION     DBBBSB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.158"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACIUS0_003678"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACIUS0_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGB0221262.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACIUS0_003679"
     CDS             complement(1433..1759)
                     /locus_tag="ACIUS0_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGB0221263.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACIUS0_003680"
     CDS             1894..2235
                     /locus_tag="ACIUS0_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGB0221264.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACIUS0_003681"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACIUS0_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACIUS0_003682"
     CDS             complement(2833..3543)
                     /locus_tag="ACIUS0_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGB0221265.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACIUS0_003683"
     CDS             3651..3956
                     /locus_tag="ACIUS0_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGB0221266.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACIUS0_003684"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACIUS0_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6299..7288)
                     /locus_tag="ACIUS0_003685"
     CDS             complement(6299..7288)
                     /locus_tag="ACIUS0_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221267.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="ACIUS0_003686"
     CDS             complement(7373..7864)
                     /locus_tag="ACIUS0_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221268.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..>8594)
                     /locus_tag="ACIUS0_003687"
     CDS             complement(8185..>8594)
                     /locus_tag="ACIUS0_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221269.1"
                     /translation="RVGIQIGKVEDNRSTEVARFMRTKAGRSLTNPENGFVKKINMGH
                     LGTSWLADPVAAIEFGRWLDLGYGLAVTRAFVSMTSSATVQSAVASAEDIKPETKEFI
                     QRHGTGLYIAGREVSQAEYSDYQAVTAGRCEWR"
BASE COUNT         2214 a         2208 c         2088 g         2084 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga gggcatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggc
//
LOCUS       DBBBSB010000157         8527 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.159, whole genome shotgun sequence.
ACCESSION   DBBBSB010000157 DBBBSB010000000
VERSION     DBBBSB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.159"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..188
                     /gene="csgC"
                     /locus_tag="ACIUS0_003688"
     CDS             <1..188
                     /gene="csgC"
                     /locus_tag="ACIUS0_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGB0221270.1"
                     /translation="SSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSD
                     GQALHLSQQWTPSSEKS"
     gene            309..620
                     /gene="ymdA"
                     /locus_tag="ACIUS0_003689"
     CDS             309..620
                     /gene="ymdA"
                     /locus_tag="ACIUS0_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGB0221271.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            715..1248
                     /gene="ymdB"
                     /locus_tag="ACIUS0_003690"
     CDS             715..1248
                     /gene="ymdB"
                     /locus_tag="ACIUS0_003690"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGB0221272.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1190..2671
                     /gene="clsC"
                     /locus_tag="ACIUS0_003691"
     CDS             1190..2671
                     /gene="clsC"
                     /locus_tag="ACIUS0_003691"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGB0221273.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="ACIUS0_003692"
     CDS             complement(2679..3836)
                     /gene="mdoC"
                     /locus_tag="ACIUS0_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGB0221274.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4230..5765
                     /gene="mdoG"
                     /locus_tag="ACIUS0_003693"
     CDS             4230..5765
                     /gene="mdoG"
                     /locus_tag="ACIUS0_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGB0221275.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5758..8301
                     /gene="mdoH"
                     /locus_tag="ACIUS0_003694"
     CDS             5758..8301
                     /gene="mdoH"
                     /locus_tag="ACIUS0_003694"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGB0221276.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2221 g         2131 t
ORIGIN      
        1 gcagttcagg gcaaagtcag acgaagcaag aaaagaccct ctcattgcct gctaatcaac
       61 ccattgcttt gacgaagttg agtttaaata tttccccgga agatcgggtg aaaatagttg
      121 ttactgtttc tgatggacag gcacttcatt tatcacaaca atggacgccc tcttcagaaa
      181 agtcttaatt tgttgaaata tcgagcataa gatgaatctg gagagaatgg tctgctgcga
      241 atcagccaac ctgaaagtat ggataacaca accttcaagg atgactaatc attgaggaaa
      301 tagaataaat gttcagacct tttttagact ctcttatgct cggcagtttg ttttttcctt
      361 ttattgccat tgcaggaagc accgcgcaag ggggcgtgat ccatttttat ggccaaattg
      421 tggaaccggc atgtgacgtc agcacccagt catcacccgt agaaatgaac tgcccacaga
      481 atggttctat tccgggcaaa acctactcca gcaaagcgtt aatgagcggc aatgtcaaaa
      541 acgcgcaaat agcctcagta aaagtgcagt atcttgataa acagaaaaag ctggctgtaa
      601 tgaacatcga atataactaa gtttctgaac aactcacgtt gctgagcaga aaaatgcgat
      661 ttaaccaaaa agcctgctgt acacttaaga aacaagaagg tgtaaggagg cgttatgaaa
      721 acgcgtattc atgttgtgca gggtgatatt accaaactgg ccgttgatgt gattgtgaat
      781 gcggctaatc cgtcattaat gggaggcggc ggcgtcgatg gggccattca tcgcgcagcg
      841 ggtccggccc tgctggatgc ctgtttaaaa gtcaggcaac agcagggcga ttgccctacg
      901 gggcatgccg ttattacgct tgcaggcgat cttcccgcta aagccgtagt acacaccgtc
      961 gggccagtct ggcgtggtgg ggaacaaaac gaagaccagc ttttgcagga tgcctatctg
     1021 aatagcctac gactggtggc ggcaaacagc tatacgtcag tggcttttcc tgcaatcagt
     1081 actggggttt atagttaccc tcgtgcggca gcggctgaaa tcgcagttaa aaccgtttca
     1141 gaatttatta cccgtcacgc tttacccgaa caggtatact ttgtctgtta tgatgaagaa
     1201 aacgcccacc tctacgaaag actccttacc caacaaggag atgaatgatt tgccccggct
     1261 ggcgagcgcg gtgctgccac tgtgttcgca acatcccggt cagtgtggtc ttttccctct
     1321 ggagaaaagt ctggatgcgt ttgccgcccg gtatcgtctg gccgaaatgg cagagcatac
     1381 gctggatgtt cagtattaca tctggcagga cgatatgtcg gggcggttac tgttttccgc
     1441 cctgttagcc gcagcaaagc gtggcgttcg cgtccgtttg ttgctggacg acaacaatac
     1501 gcccggactt gacgacattt tgcgcttgct tgacagtcat ccacgcattg aagtccggct
     1561 ttttaatcct ttctcgtttc gcttgctacg tccgcttgga tatatcaccg acttttcccg
     1621 tctcaatcgc cggatgcaca ataaaagttt cactgtcgat ggcgtggtta ccctagtggg
     1681 aggacgaaat attggtgatg cctattttgg tgcaggggaa gagccacttt tttcggattt
     1741 agatgtcatg gcaataggcc ccgtggtaga ggatgttgcc gatgatttcg cccgctactg
     1801 gtattgcaaa tcggtttcac ccttacagca ggtgctggat gtcccggaag gtgaaatggc
     1861 ggatcgcatc gagttacccg cctcctggca taacgatgcc atgacgcatc gttatttacg
     1921 caaaatggaa tccagtccat ttataaatca tctggttgat ggaacgttgc cgcttatctg
     1981 ggcgaagaca cgtttattaa gtgatgatcc ggcgaaaggg gagggcaagg caaaacggca
     2041 ttcactgtta ccgcagcgcc tgttcgatat catgggctca cccagtgaac gcatcgatat
     2101 tatctcttcc tattttgtac cgacacgcgc aggtgtggcg caactcttac ggatggtgag
     2161 aaaaggggta aagattgcga tcctaaccaa ttctcttgcc gctaacgatg ttgctgtcgt
     2221 ccatgccgga tacgcgcgct ggcgcaaaaa attgctccgc tatggcgtgg aattatatga
     2281 actcaagccg acgcgtgaac aaagcagtac gttacacgat cgcggcataa ccggtaattc
     2341 cggtgccagc ctgcatgcta aaacttttag catcgatggt aaaacggtgt ttatcggctc
     2401 tttcaatttc gatccgcgtt caacattgct caatactgaa atgggcttcg tgatagagag
     2461 cgaaacgctg gcacagttaa ttgataaacg ctttattcag agccagtacg atgcggcctg
     2521 gcagctccgt ctggacaggt ggggacggat caactgggtt gatcgtcatg caaagaaaga
     2581 ggttattctc aaaaaagaac ccgccaccag tttctggaag cgggttatgg tcagactggc
     2641 gtcgatattg cccgtggaat ggttattgta aatgtggctt aacgggctgg tgctttatcg
     2701 ttctcacgct taacaaccgg tttaccagag aacaaaaact tcagtaacgg gatgcgtaaa
     2761 tgaatttcat acagaattat cgcaatccct actacgaata tcaggccaca gagaaaacca
     2821 agccagttgg aggtgatgtg cggtgtaatg tatgcgccga aaaacagcgt taacgggtgg
     2881 tgaaccagat agataaacag cgatgcgtta acaaaataag tcacccgcgc tgactggaag
     2941 ttaagcaaac ggtggccaaa ggagaagacc acattcacca tccacaggcc gaggaccatg
     3001 gtgatcaccg actcggtttc gtacatccag gcatcgccac tgccatagcg ctggttgagt
     3061 aaataagcga caaacgccaa tgctgcagcg agggtacagc cacgagacgg cgtggtaaac
     3121 aaggctttaa gatgagggaa aatgaaagcc agtgcgccga ggataaagaa cggtaaataa
     3181 aatagcgttt gcatgacaat aaaattgaac atgccattac tcagaatggg tggatacaca
     3241 ataaaaatcg ttcttcttat taccgcatag ccgatgccga ggcataaaaa aatcaccgat
     3301 agttttacca tcgagaattt tttattcgtt ttatcagaat tttctaaatt atttctgatg
     3361 cgcttaaata tccatacaca cagcgtcgtc atgactacta acaccagtaa aaaccacagg
     3421 tgtgatatta attcccaggc caacgtatta tatttgtcat acaatgatag cccaggccaa
     3481 ctttccgctt tccctttgac atattgcagc ataataaatt gcggtaatgt cagtaggggg
     3541 atggctgtta acatcgggat acctacacgt tcgacacgta ctttccacca ttttttcaag
     3601 ggatagcgta aaaaaagcat gtaggaaaag tatccggata taacgaaaaa tacctgcatg
     3661 cggaacgagt ggatgaagtc attaaaaagg gttagccaca atgacggttc ggcgctattc
     3721 acatgccatg tatggctcga atagattaaa gaaatatgaa aagggatccc taacaacatc
     3781 agccaggcgc ggatggagtc gaggaaatat tcacgttgcg cgggtactgg attcatatat
     3841 ggttaactaa tctcggattt ttcgtcttat ccctgtcggg ttatgccttt aggcttgttg
     3901 ccatagcgac accgacctga ccgcgccagg cgcaggcttc aaggttttta tgcatagcat
     3961 catcgctacc actaaccaga atggaagcgt ctgtaagacg gttgataaat aaatttgctg
     4021 gcaaacccta cacgaagtcg atgcttctgt ctttaggaga agcacggaaa gtgaaaacgg
     4081 ttgcaatcag gtgcttaatc catgagccag cgtgctgaac gataccggga ttcagttgtc
     4141 ggaatggctg gttatccatt aaaatagatc ggatcgatat aagcacacaa agggggaagt
     4201 gcttactaat tatgaaacat aaactacaaa tgatgaaaat gcgttggttg agtgctgcag
     4261 taatgttaac cctgtataca tcttcaagct gggctttcag tattgatgat gtcgcaaagc
     4321 aagctcaatc cttagccggg aaaggctatg aggcgcctaa aagcaacttg ccctccgttt
     4381 tccgcgacat gaaatacgcg gactatcagc agatccagtt taatcatgac aaagcgtact
     4441 ggaacaatct gaagacccca ttcaaactcg agttctacca tcagggtatg tacttcgata
     4501 ccccggtcaa aataaatgaa gtgactgcca ccgcagtcaa acgaatcaaa tacagcccgg
     4561 attatttcac tttcggcgat gttcagcatg acaaagacac ggtaaaagac cttggttttg
     4621 ccggttttaa agtgctttac ccgatcaaca gcaaagataa aaacgatgaa atcgtcagca
     4681 tgctcggggc cagctatttc cgcgtgattg gtgcaggtca ggtttatggc ctttctgccc
     4741 gcggcctagc aattgatacc gctttgccat cgggtgaaga atttccacgc ttcaaagagt
     4801 tctggatcga gcgtccaaaa ccgactgata aacgtttaac catttatgca ttgcttgact
     4861 cgccgcgtgc gacaggtgct tacaaattcg tggttatgcc agggcgtgac acggttgtgg
     4921 atgtgcagtc gaaaatctat ctgcgcgata aagtcggcaa actgggggtt gcaccgttaa
     4981 ccagtatgtt cctgtttggg ccgaaccaac cgtcgcctgc aaataactat cgtccggagt
     5041 tgcacgactc taacggtctg tctatccatg ctggtaatgg cgaatggatc tggcgtccgt
     5101 tgaataaccc gaaacattta gcggtcagca gcttctccat ggaaaacccg cgaggctttg
     5161 gtctgttgca acgaggtcgt gatttctccc gctttgaaga tctcgatgat cgttacgatc
     5221 tccgtccaag cgcatgggtg actccgaaag gggagtgggg caaaggcagc gttgagctgg
     5281 tggaaattcc aaccaacgat gaaaccaacg ataacatcgt cgcttactgg acgccggatc
     5341 agctgccgga gccgggtaaa gagatgaact ttaaatacac catcaccttc agccgtgatg
     5401 aagacaaact gcatgcgcca gataacgcat gggtgcaaca aacgcgtcgt tcaacggggg
     5461 atgtgaagca gtcgaacctg attcgccagc ctgacggtac tatcgccttt gtggtcgatt
     5521 ttaccggcgc agagatgaaa aaactgccag aggatacccc ggtcacagcg caaaccagca
     5581 ttggtgataa tggtgagata gttgaaagca cggtgcgcta taacccggtt accaaaggct
     5641 ggcgtctggt gatgcgtgtg aaagtgaaag atgccaagaa aatcactgaa atgcgtgctg
     5701 cgctggtgaa tgccgatcag acgttgagtg aaacctggag ctaccagtta cctgccaatg
     5761 aataagacaa ctgagtacat tgacgcaatg cccatcgccg caagcgagaa agcagcattg
     5821 ccgaagactg atatccgcgc cgttcatcag gcgctggatg ccgaacaccg cacctgggcg
     5881 cgggaggatg attccccgca aggctcggta aaggcgcgtc tggaacaagc ctggccagat
     5941 tcacttgctg atggacagtt aattaaagac gacgaagggc gcgatcagct taaggcgatg
     6001 ccagaagcaa aacgctcctc gatgtttccc gacccgtggc gtaccaaccc ggtaggccgt
     6061 ttctgggatc gcctgcgtgg acgcgatgtc acgccgcgct atctggctcg tttgaccaaa
     6121 gaagagcagg agagcgagca aaagtggcgt accgttggta ccatccgccg ttacattctg
     6181 ttgatcctga cgctcgcgca aactgttgtc gcgacctggt atatgaagac cattcttcct
     6241 tatcagggtt gggcgctgat taatcctatg gatatggttg gtcaggattt gtgggtttcc
     6301 tttatgcagc ttctgcctta tatgctgcaa accggtatcc tgatcctttt tgcggtactg
     6361 ttctgttggg tgtccgccgg attctggacg gcgttgatgg gcttcctgca actgcttatt
     6421 ggtcgcgata aatacagtat atctgcgtca acagttggcg atgaaccatt aaacccggag
     6481 catcgcacgg cgttgatcat gcctatctgt aacgaagacg tgaaccgtgt ttttgctggc
     6541 ttgcgtgcaa cgtgggaatc agtaaaagcc accgggaatg ccaaacactt tgatgtctac
     6601 attctgagtg acagttataa cccggatatc tgcgtcgcag agcaaaaagc ctggatggag
     6661 cttatcgctg aagtcggtgg cgaaggtcag attttctatc gccgccgccg tcgccgcgtg
     6721 aagcgtaaaa gcggtaatat cgatgacttc tgccgtcgct ggggcagcca gtacagctac
     6781 atggtggtgc tggatgctga ctcggtaatg accggtgatt gtttatgcgg cctggtgcgc
     6841 ttgatggaag ccaacccgaa cgccgggatc attcagtcgt cgccgaaagc gtccggtatg
     6901 gatacgctgt atgcgcgctg tcagcagttc gcgacccgcg tgtatgggcc actgtttaca
     6961 gccggtttgc acttctggca acttggcgag tcgcactact ggggacataa cgcgattatc
     7021 cgcgtgaaac catttatcga gcactgcgca ctggctccgc tgccgggcga aggttccttt
     7081 gccggttcaa tcctgtcaca tgacttcgta gaagcggcgt tgatgcgccg tgcaggttgg
     7141 ggggtctgga ttgcttacga tctcccgggt tcttatgaag aattgccgcc taacttgctt
     7201 gatgagctaa aacgtgaccg ccgctggtgc cacggtaacc tgatgaactt ccgtctgttc
     7261 ctggttaagg gtatgcaccc ggttcaccgt gcggtgttcc tgacgggcgt gatgtcttat
     7321 ctctccgctc cgctgtggtt tatgttcctc gcgctctcta ctgcattgca ggtagtgcat
     7381 gcgttgaccg aaccgcaata cttcctgcaa ccacggcagt tgttcccggt gtggccgcag
     7441 tggcgtcctg agctggcgat tgcacttttt gcttcgacca tggtgctgtt gttcctgccg
     7501 aagttattga gcattttgct tatctggtgc aaaggaacga aagaatacgg cggcttctgg
     7561 cgcgttacat tatcgttgct gctggaagtg ctgttttccg tgctgctggc tccggtacgc
     7621 atgctgttcc atacggtctt cgttgtcagc gcgttccttg gctgggaagt ggtgtggaat
     7681 tcaccgcagc gtgatgatga ctccacttca tggggcgaag cgttcaaacg ccacggctca
     7741 cagctgctgt tggggttagt gtgggctgtt gggatggcgt ggctggatct gcgtttcctg
     7801 ttctggctgg caccgattgt cttctcgttg atcctgtcac cgtttgtttc ggtgatttcc
     7861 agccgtgcca ccgttggtct gcgcaccaaa cgctggaaac tgttcctgat cccggaagag
     7921 tattcaccgc cgcaggtgct ggttgatacc gatcggttcc ttgagatgaa tcgccaacgc
     7981 tcccttgacg atggctttat gcacgcagtg tttaacccgt catttaacgc tctggcaacc
     8041 gcaatggcga cagcgcgtca ccgcgccagt aaggtgctgg aaatcgcccg tgaccgccac
     8101 gttgaacagg cgcttaacga gacgccagag aagctgaatc gcgatcgtcg cctggtgctg
     8161 ctaagcgatc cggtgacgat ggcccgtctg catttccgcg tctggaattc ccctgaaaga
     8221 tattcttcat gggtgagtta ttacgaaggg ataaagctca atccactggc attgcgtaaa
     8281 ccggatgcgg cttcgcaata aaaacgtagt tgcctgatgc gctacgctta tcaggcctac
     8341 atcgttcctg caatttattg attttgcaag attttgtagg tcggataagg cgttcacgcc
     8401 acatccggca aaaacaacga gccaataaaa ataccggcgt tatgccgtaa tgccagtcag
     8461 ttaagcaact gactggctct ttttcggggc tgtggggtat ttccagggcc tctcctttac
     8521 cactctc
//
LOCUS       DBBBSB010000158         8489 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.160, whole genome shotgun sequence.
ACCESSION   DBBBSB010000158 DBBBSB010000000
VERSION     DBBBSB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.160"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            44..397
                     /locus_tag="ACIUS0_003695"
     CDS             44..397
                     /locus_tag="ACIUS0_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGB0221277.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACIUS0_003696"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACIUS0_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGB0221278.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACIUS0_003697"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACIUS0_003697"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGB0221279.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACIUS0_003698"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACIUS0_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGB0221280.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACIUS0_003699"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACIUS0_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGB0221281.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACIUS0_003700"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACIUS0_003700"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGB0221282.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACIUS0_003701"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACIUS0_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGB0221283.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACIUS0_003702"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACIUS0_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGB0221284.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACIUS0_003703"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACIUS0_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGB0221285.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8489
                     /locus_tag="ACIUS0_003704"
                     /pseudo
     CDS             7609..8489
                     /locus_tag="ACIUS0_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1903 a         2305 c         2285 g         1996 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaata
//
LOCUS       DBBBSB010000159         8376 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.161, whole genome shotgun sequence.
ACCESSION   DBBBSB010000159 DBBBSB010000000
VERSION     DBBBSB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.161"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..958
                     /gene="espL1"
                     /locus_tag="ACIUS0_003705"
                     /pseudo
     CDS             1..958
                     /gene="espL1"
                     /locus_tag="ACIUS0_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1130..1237
                     /gene="yniD"
                     /locus_tag="ACIUS0_003706"
     CDS             1130..1237
                     /gene="yniD"
                     /locus_tag="ACIUS0_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGB0221286.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1290..2048)
                     /locus_tag="ACIUS0_003707"
     CDS             complement(1290..2048)
                     /locus_tag="ACIUS0_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGB0221287.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2335..3264
                     /gene="pfkB"
                     /locus_tag="ACIUS0_003708"
     CDS             2335..3264
                     /gene="pfkB"
                     /locus_tag="ACIUS0_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGB0221288.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3365..3655
                     /gene="ghoS"
                     /locus_tag="ACIUS0_003709"
     CDS             3365..3655
                     /gene="ghoS"
                     /locus_tag="ACIUS0_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGB0221289.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3761..4621
                     /locus_tag="ACIUS0_003710"
     CDS             3761..4621
                     /locus_tag="ACIUS0_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGB0221290.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4662..5198)
                     /locus_tag="ACIUS0_003711"
     CDS             complement(4662..5198)
                     /locus_tag="ACIUS0_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGB0221291.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5345..6013
                     /gene="hxpB"
                     /locus_tag="ACIUS0_003712"
     CDS             5345..6013
                     /gene="hxpB"
                     /locus_tag="ACIUS0_003712"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGB0221292.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6176..6766
                     /locus_tag="ACIUS0_003713"
     CDS             6176..6766
                     /locus_tag="ACIUS0_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGB0221293.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6899..8289
                     /gene="tcyP"
                     /locus_tag="ACIUS0_003714"
                     /pseudo
     CDS             6899..8289
                     /gene="tcyP"
                     /locus_tag="ACIUS0_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8293..>8376)
                     /locus_tag="ACIUS0_003715"
     CDS             complement(8293..>8376)
                     /locus_tag="ACIUS0_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221294.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         2173 a         2004 c         2009 g         2190 t
ORIGIN      
        1 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
       61 ttacctgtgg gagttctagg ggctattttc gcgggtattg catcacgaat gccatttgcc
      121 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
      181 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
      241 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
      301 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
      361 aatctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ttatatctcc
      421 atgttgtatg gattcgatga aataatcgat atctttctga atacattagc cactccaata
      481 gcactaagag cttttaaaca aaaaactggt gatgagtatt ttagccatga aaatacatga
      541 tggtgagcca ggattatacg ccgcaatgga aaataatcac cctttgtgtg tcacacggtt
      601 cctctctaaa attaatggca tcgcctttaa atacaagttg agcaaagcta acatcatgga
      661 tttattaaaa ggagctacag cacagggaac ccctgcatta tacatcgcca tgagcaaggg
      721 taatgaagac gtcgtgttat cttatatatc gacgctgggt gcttttgcaa aaaaacattc
      781 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
      841 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
      901 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
      961 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
     1021 gaccatgtgc acaacgccac ccgacggctg taataacaaa aaatcccttg atgcctgtcc
     1081 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
     1141 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
     1201 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
     1261 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
     1321 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
     1381 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
     1441 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
     1501 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
     1561 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
     1621 aaaaactgac gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
     1681 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaagcggcta
     1741 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
     1801 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
     1861 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
     1921 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
     1981 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
     2041 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
     2101 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     2161 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     2221 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     2281 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     2341 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     2401 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     2461 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     2521 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2581 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2641 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2701 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2761 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2821 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2881 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga atcgcgaact cacccagcca
     2941 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     3001 gtttcccttg gtccacaagg agcgctgggt gtcgatagtg aaaactgcat tcaggtggtg
     3061 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     3121 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     3181 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     3241 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     3301 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     3361 aactatggcc agtggcgatc ttgtccatta tgtcataacc gtcatgttgc atgaggatac
     3421 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     3481 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3541 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3601 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3661 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3721 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3781 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3841 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3901 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3961 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     4021 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     4081 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     4141 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     4201 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     4261 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     4321 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     4381 cattttcgac cctgcctgcg actggggtga ccgagagtgc gacctagcga tgttaccgct
     4441 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     4501 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4561 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4621 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4681 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4741 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4801 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4861 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4921 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4981 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     5041 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     5101 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     5161 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     5221 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     5281 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     5341 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     5401 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     5461 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     5521 tctttggtac gcccggcaac cgtggaatgg gccaagccgt caggaagtag tagaacgggt
     5581 tattgcccgt gccatttcac tggttgaaga gacacgtcca ttattaccag gcgtgcgcga
     5641 agctgttgcg ttatgcaaag aacaaggttt attggtggga ctggcctccg cgtcaccact
     5701 acatatgctg gaaaaagtgt tgaccatgtt tgacttacgc gacagtttcg atgccctcgc
     5761 ctcggctgaa aaactgcctt acagcaagcc gcatccgcaa gtatatctcg actgcgcagc
     5821 aaaactgggc gttgacccac tgacctgcgt ggcgctggaa gactcggtaa atggcatgat
     5881 cgcttctaaa gcagcccgca tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga
     5941 tccacgtttt gtattagcaa acgtcaaact ctcatcgctg acagaactca ccgcaaaaga
     6001 ccttctcggt taatgaccag gggcagtgat cacgctgccc ctggttcttt atctgaattt
     6061 gcattcaact gacggattaa tcgtcaattt aagagaaaga gttacaccgt caccacttcc
     6121 gtgcactgta taaaaatcct atactgtacg tatcgacagt ttagtgagtt ttatcatgac
     6181 ggcggaaggt caccttctct tttctattgc ttgtgcggta tttgccaaaa atgccgagct
     6241 gacgcccgtg ctggcacagg gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg
     6301 tttgttaccg gacatcgatc acccaaagtc gtttcttggg cagcgattaa aatggatatc
     6361 aaaaccgatc gcccgcgctt ttgggcatcg tggttttacc cacagtctgc tggcggtatt
     6421 tgcgctgctg gcaacctttt accttaaggt tccggaaggc tggttcattc cggctgatgc
     6481 gctacaagga atggtgctgg gttatttgag ccacatactt gccgatatgc tgacacccgc
     6541 cggtgttccc ctgctctggc catgccgctg gcgtttccgc ttgcctatcc tggttcccca
     6601 aaagggcaac caactggaac gttttatctg catggcatta tttgtctggt cggtatggat
     6661 gccccattca ttacccgaga acagcgctgt tcgttggtca tcgcaaatga tcaatacctt
     6721 gcagatccag tttcatcggc ttattaagca tcaggttgaa tactaaaaag gcaaaaatca
     6781 cctttctgga ataagcaatt ccatttgaat ataagagcca gctcacagtt ctgttaatct
     6841 tgcgccaaca ctatgactgc tacgcagtga tagaaataat aagatcagga gaacggggat
     6901 gaactttcca ttaattgcga acatcgtggt gttcgttgta ctgctgtttg cgctggctca
     6961 gacccgccat aaacagtgga gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt
     7021 ggtttttggc cttgccctgc ataccattta tggttctgac agccaggtac ttaaagattc
     7081 tgtacagtgg tttaacatcg ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat
     7141 gccgttagtc ttcgcctcta ttctgagcgc ggttgcccgt ctgcataacg catctcagtt
     7201 aggcaaaatc agttttctga ccatcggtac gcttttgttt accacgctga ttgcggcgct
     7261 ggtcggtgtg ctggtcacca acctgtttgg tttgacggct gaaggtctgg ttcagggtgg
     7321 tgcagaaact gcacgtctga acgccattga aagtaactat gttggtaaag tctctgatca
     7381 gagcgttccg cagctggtct tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg
     7441 agccaatccg acgtcaatta tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc
     7501 attgaaactg ctgaaggatg atgcgccgaa aggggaacgc gtcttaaccg ctatcgatac
     7561 cctacaaagc tgggtgatga aactggttcg ccttgtcatg cagttgaccc cttacggcgt
     7621 tctggctcta atgaccaaag tggttgcagg ttctaacttg caagacatca tcaaactggg
     7681 aagtttcgtt gtcgcgtcct acctcggtct gctgattatg tttgctgtgc atggcattct
     7741 gctgggcatt aatggcgtga gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac
     7801 gtttgccttt accagccgtt ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac
     7861 gcgtcgtctg ggcgttcctg aatccatcgc cagtttcgcc gcctctttcg gtgcaaccat
     7921 tggtcagaac ggctgcgccg gtttgtatcc ggcaatgctg cggtgatggt tgcgcctacg
     7981 gttggcatta acccgctgga cccgatgtgg attgcgacgc tggtcggtat tgttaccgtt
     8041 agttccgcag gcgttgccgg tgtcggtggt ggtgcaactt tcgccgcact gattgtgctg
     8101 cctgcgatgg gcctgccagt aaccctggtg gcgctgttaa tctccgttga accgcttatc
     8161 gacatgggcc gtacggcgct aaacgttagt ggctcgatga cagctggcac gctgaccagc
     8221 cagtggctga agcaaaccga taaagccatt ctggatagcg aagacgacgc cgaactggca
     8281 caccgttaat tcttatgctg gcaacggtcc gttttgtata ggggccgttg ctttactttt
     8341 taaatgttcc atcgcggcga tgcatactga ctcata
//
LOCUS       DBBBSB010000160         8095 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.162, whole genome shotgun sequence.
ACCESSION   DBBBSB010000160 DBBBSB010000000
VERSION     DBBBSB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.162"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..248)
                     /locus_tag="ACIUS0_003716"
     CDS             complement(<1..248)
                     /locus_tag="ACIUS0_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221295.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACIUS0_003717"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACIUS0_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACIUS0_003718"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACIUS0_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACIUS0_003719"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACIUS0_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACIUS0_003720"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACIUS0_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HGB0221296.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACIUS0_003721"
     CDS             complement(2964..3278)
                     /locus_tag="ACIUS0_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221297.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3375..3485)
                     /locus_tag="ACIUS0_003722"
                     /pseudo
     CDS             complement(3375..3485)
                     /locus_tag="ACIUS0_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3809..4486)
                     /gene="ospD1"
                     /locus_tag="ACIUS0_003723"
     CDS             complement(3809..4486)
                     /gene="ospD1"
                     /locus_tag="ACIUS0_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HGB0221298.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4858..4995)
                     /locus_tag="ACIUS0_003724"
                     /pseudo
     CDS             complement(4858..4995)
                     /locus_tag="ACIUS0_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5077..5375)
                     /locus_tag="ACIUS0_003725"
                     /pseudo
     CDS             complement(5077..5375)
                     /locus_tag="ACIUS0_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5964..6123)
                     /locus_tag="ACIUS0_003726"
                     /pseudo
     CDS             complement(5964..6123)
                     /locus_tag="ACIUS0_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6127..7356)
                     /locus_tag="ACIUS0_003727"
                     /pseudo
     CDS             complement(6127..7356)
                     /locus_tag="ACIUS0_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7376..7723)
                     /gene="tnpB"
                     /locus_tag="ACIUS0_003728"
     CDS             complement(7376..7723)
                     /gene="tnpB"
                     /locus_tag="ACIUS0_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGB0221299.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7723..>8095)
                     /gene="tnpA"
                     /locus_tag="ACIUS0_003729"
     CDS             complement(7723..>8095)
                     /gene="tnpA"
                     /locus_tag="ACIUS0_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HGB0221300.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         2074 a         1888 c         1776 g         2357 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gtggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgatcc tacccacgta
     3361 atatgaacac aggcttaagt gaggttctgg ttttcaaatt gttcaggact gagacctcca
     3421 caccaactgt gccgtcgtca gcgattgtaa tcgcacttga tataattaaa cactgtatat
     3481 ttcatttgtg gagtctccag tctgagaggg ctttatgcat tccgtattgg gcactatgat
     3541 gccagaaatc tgcaaggctc cacaccttcc tgtatttccc ttcctctatc acagtgcact
     3601 ttacttaata gggtgggatg tgttcattac atttattgtg ttttttatga ttgtttttgc
     3661 gtccagtgtc gttcgtcatg ggaaactggt tctatgggta gtatcgctca gtctagttaa
     3721 ctgtttgttt ctttttagaa attaagcaga tcacacaatt cggggagaga tccttctaag
     3781 tgcaattctt tgcagctatt tagcacactt aaaacaactc ttgtattttg tcagaaataa
     3841 tattaaacat gtcttgttca ttattgttaa gtctgtctgc tatttctgta agttttatcc
     3901 cactatgatg gcatatattt atatatgcca tcatagcctc gatattccct ttccctgcgg
     3961 ccagaaataa tcctggtaac ccctcattat ctcttacatc caatagttta atcgtttctt
     4021 ttggtgataa atcaaaagtt ttaataaaat ctccataagc ttgaatcgca gcggaatgac
     4081 cttcttgaag agctatgaac aaagcagatt ccccagcatt atttcgtgct gcaagaactt
     4141 ctagtttttc ctctttgggt atttcggctg ccaacagcga ttcaccgtat ttacgcgctg
     4201 catcggcatt tccatgccaa atcaatgaaa acaaattatg gcggttatac tccttattat
     4261 tctttgtcgg aatggatatg ttctgtattt tggccaattt tctgaaagaa acaccactat
     4321 atcccccccc tgatttttct tcattgatgg cgtgggatat agcggtattt gtcacgttaa
     4381 tgatattatt tttcattcct ttattttcat tagcagtatt gctgcctatt attaagtgca
     4441 tatttttgtt gtttgctgga tgtaatccat agttatttat tgacattttt gttcatcctt
     4501 tttgctaaaa aaaaacaata acctattttt gtaatgtaat agatattagt ctattgctaa
     4561 tctaaaaaaa ccattgccac attgaatggc tatattcacc aataaattct ttcgatttcg
     4621 tatattgaca tatcgtagat atatcttatc cctaagattc aataatcgag tccgtttacc
     4681 attctgtgca agcgccttca ctcagaagtg accgtcaagg cgatcaccaa acttgataat
     4741 aaagcagctc attgccatac gccagtcctt aaggggcgtt gtccatttct gtgatgctgc
     4801 ctagatttaa ctgccagctc cggcttatct acatctaaat atgtgagcct aataccgcta
     4861 cactttgtcc agactatgtt tttctccggg aagtgggcta accgtctcca taaagtgacg
     4921 aacttttttc aacagtggcc atattgaacg gcactgatga ttacgggtta ttgtctcatg
     4981 aagagccggc cacatcactg acagcagtga cttcaggcct tcagcaccag acagcctgaa
     5041 ccagttaatc catcgcccga caaatttagt aatctagggc tggtttttct tcagctgttg
     5101 cggttcgaaa gtgccattac gatcacgcag agtacggatt tccagaggtt catctccagt
     5161 agtgataatc ttcgttaagc aaccattgcg ggaattgctg ccagatctaa gctgattttt
     5221 ctcgcaccca agatgatgag tcatattagc attgagtacg gtttcatttt cagcatccaa
     5281 tcaaactgac tgagatcttc aggatttttg agacttttgg ctagttcatt agctaaagcc
     5341 cgcaacttat tagatgtcaa ctgtttttca ttcataaatt caccttcatt tgatatcaaa
     5401 ttaaatagtt caacatcaga gaattataca aacttatgta caggctccta tgatcttcga
     5461 atcctgaaga ttatagtgaa aataagtttt cacgttgaaa tcccacatag ttagtatatg
     5521 ataaataatc atggatgacc tgattaacct cttggggtag tgcttttata gaagaataaa
     5581 tattctacta atttaataca tcacctaaaa tagtattgtt ttttatggtt ggttgaaggt
     5641 cgtggtgatg atgttagtcc ttttgagaat attccagtgc cagatcttac ttataataaa
     5701 ggggcttggc agtttggata aactattgtt cttattccac taatacaaac tttttgtaaa
     5761 gtatattaaa tatacttcat ttcatactga aatccccgga agtattcaat caaacaatta
     5821 caccataaaa ggaagagggg gccctaatta agttgtcaag catgtttatg atacctgcag
     5881 ggtataaatt ttctggctgc aagcagacac atataaaacg tctttattga tcctacccac
     5941 ataatatgga cacagtcgta agtgaggctc tggttttcaa attgttccgg actgagacca
     6001 ccacaccaac ggtgccgtcg ccagcgattg taatcgcact cgatataact aaacactgtt
     6061 gcccgcacta tttcccgatg gataatagtg ttctccgtgg agacattcca ttttcagcga
     6121 atgaattttc atctgttgct gtatccagtc atacagtgac tgcatcagtg gcgcggctct
     6181 ggcttttctt gccgccagac gctgttctgc tgaacatccc cggacttctg cctcgatggc
     6241 atacagttca ccgatacgct gcagggcttc cgtggtgatg tcggttggca ctcttgcatg
     6301 cacatcgtgg atttttctcc ggacatgagc catacatgcg gcttccgtta ttctgccgga
     6361 ttcgtacaac gcccggtaac caccgtaagc atcggcctga agcactccgc tgtaaccggc
     6421 cagatgattc tgtggatgga tacctttccg gtccggtgag tacgcgaacc agaccgccgg
     6481 aggcatttcc gaaccggcgt tacggtcatc acgaacgtag acccacagcc gggcagtcct
     6541 tgttttaccg ctgcccggct cctggaccgg aacggggata tcatcggcat ggactttacc
     6601 gggcatcagc acatactggc gcaggatgtc atacagcggt tccagcagtt cagcaacagc
     6661 acctgtccag cgccccagtg tggcacggct cagctccacg ccctgacgac ggtatatttc
     6721 tgactggcgg tataacggca gatagtctgc atattttcca gcaacaacat gggccagaag
     6781 ccccgctccg gcataactgc gtgcaatggg ttttgaaggc accggtgcct gcacgatatg
     6841 gtcacaccgg caacaggcca gtttcggacg ttgtgtttcg ataaccttaa aggcgctgct
     6901 gataagctcc agctgctctg acacatcaca tcccagagaa ctgagttcgc caccacaggc
     6961 cgggcagcat tcctcttccg gacggatgac ccgggtttca cggggaagtg aggctggtaa
     7021 cggtttacgg gctgaagact ggcgcagggg agatggcagt accgggtcat attgctcacc
     7081 cagcgtttcc gccatttctt cctgaagtgc gctgattcgc tcctgtgctt ccagtatctg
     7141 ccgttcggtt tttgcgcgaa gtttttctga gcttttaccg aactgcatgc gctgcagttt
     7201 cgcaaccagc gccttcagcc ggttgatttc ggaagcataa gccgccaccc gctgtgagag
     7261 caggcggttg tatttcgcca tctggcggat ggtgtcctgt tgcgtctgca acagtgcccg
     7321 caggcgggca ttctcatgag caagtgaggt gtccatatcc tcactttaca acgggttata
     7381 tgcggatgcc agcgcgttcc gttcgtttcg ggtgtttcca gtcgatgccc tcaagaagca
     7441 tggataactg ggccggagta aggtgcacct tgccgtcacg ggtgaccggc cagacgaagc
     7501 ggccccgctc caggcgtctg gtaaagaggc acagtccgtc actgtcagcc cacaacactt
     7561 ttatctggtc accccggcgt ccgcggaaga tgaacagatg cccggagaac gggtcatcct
     7621 tcaggacgtt ctgaactttt gagaccaggc cgttaaagcc atttcgcata tcggtgatac
     7681 ctgcaaccag ccagatacgc gaacctgcag ggaaagatat catcagtggc tgctcccttt
     7741 tatttcgcgg ataagtatct gtaataacgc cggcgtcagt ttacctttaa gcctgagagt
     7801 tccggccggc agaaccagct cacagcacag actgtcggac ggtgtattta tctgctctgg
     7861 ttcctgtgcc ggagccggga ttttattatc cggctccggc gttaacgtca cgggaagcag
     7921 tgcaggcata ttttttccgg aaggcagcag gccacctttc cggtattgat ggcgccagtt
     7981 gaagagcagg ttatcgttga ttccgttttc acgggcgatc tgcgccacac aggctccggg
     8041 ctgcagtgac tgctccacta aagcgatttt aaattcataa ggaaaattag gccgc
//
LOCUS       DBBBSB010000161         8092 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.163, whole genome shotgun sequence.
ACCESSION   DBBBSB010000161 DBBBSB010000000
VERSION     DBBBSB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.163"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACIUS0_003730"
     CDS             complement(437..922)
                     /gene="sixA"
                     /locus_tag="ACIUS0_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGB0221301.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACIUS0_003731"
     CDS             complement(1125..3269)
                     /gene="fadJ"
                     /locus_tag="ACIUS0_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGB0221302.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACIUS0_003732"
     CDS             complement(3269..4579)
                     /gene="fadI"
                     /locus_tag="ACIUS0_003732"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGB0221303.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4759..5043)
                     /locus_tag="ACIUS0_003733"
     CDS             complement(4759..5043)
                     /locus_tag="ACIUS0_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGB0221304.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5415..6755
                     /gene="fadL"
                     /locus_tag="ACIUS0_003734"
     CDS             5415..6755
                     /gene="fadL"
                     /locus_tag="ACIUS0_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGB0221305.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7121..>8092
                     /gene="yfdF"
                     /locus_tag="ACIUS0_003735"
     CDS             7121..>8092
                     /gene="yfdF"
                     /locus_tag="ACIUS0_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGB0221306.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKV"
BASE COUNT         2199 a         2035 c         1823 g         2035 t
ORIGIN      
        1 acatccttaa aaaatacgga ataagtattt agaaaaaata gaaagtatat caataatgaa
       61 tatcaacagc ttaaaaaaca aacaatgaac atataatgcg attggcattt atccactttt
      121 gacaatataa tatataaaca ctatagccag aatttaatca agtttcaatt acatcctgcc
      181 tgattattca actctatttt ctctattatc tgtattaata aatgacaatg agtgtcaaac
      241 agatattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgt agaagaataa
      301 tttccgaatg aatgtagaaa gtgttttatt tatagataag gagagtcttc gatagtagtg
      361 aggttgacag atatccgctg cctaatgagc aattatgccc aatgaatcta cctcattggg
      421 cataatttgg aacacatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      481 agttgaatgt accgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      541 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      601 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      661 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      721 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      781 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
      841 aaccattagt ggtcagagga cgaaccgaat cactggcgga atcgagggct gcgtcgccgt
      901 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg cccgttttct
      961 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
     1021 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
     1081 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg caggtcagtt
     1141 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
     1201 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1261 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1321 accgcaccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1381 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1441 tgcccttgtg cgccaatgag cgggtaaatg gcaggatcga cctgtttttt gcttttacgc
     1501 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1561 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1621 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1681 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1741 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1801 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     1861 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccacc
     1921 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     1981 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     2041 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     2101 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     2161 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     2221 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2281 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2341 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2401 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2461 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2521 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2581 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2641 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2701 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2761 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     2821 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     2881 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     2941 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     3001 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     3061 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     3121 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     3181 gtattcattt tctcatccgg tacgtcgatg gtgatgatag caatgttgtc cagacgaaca
     3241 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3301 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3361 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgtccg taagcaatcg
     3421 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3481 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3541 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3601 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3661 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3721 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3781 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     3841 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     3901 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     3961 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     4021 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     4081 cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     4141 catcaaccag cacgtacgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     4201 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4261 caaccgcttg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4321 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4381 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4441 cgcctaccac catcttccct aaatcaaccg tgggaatgcc atgaaaagcc gtcgcctggc
     4501 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4561 gtggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4621 atcatagtct taaccatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4681 atgacacagt gaagacagaa gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4741 gtacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4801 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     4861 gattttgcac ggttcggatt tcacgctacg ggatttttca gtcagcgccg ccagcgtttg
     4921 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     4981 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     5041 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     5101 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     5161 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     5221 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5281 aacattctag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5341 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5401 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5461 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5521 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5581 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5641 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5701 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5761 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     5821 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     5881 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccatctacg
     5941 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     6001 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     6061 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     6121 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     6181 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6241 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6301 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6361 atagcctggc ttacaccagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6421 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6481 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6541 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6601 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6661 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6721 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6781 cctgctatgc aggtgacttt aactccccca cttcaccgaa agtagtttcc tcattgcttg
     6841 cctcctgagt tttgcaaaca gcctgatggc agcttgccct ttcaaatcag taagtggtat
     6901 ccacagacaa acctgaaggg aaatgcatat tttcaagcgt tctgctcgtc cttctcaaag
     6961 agtttacttt tctgcatttc caggatactc aacccccctg gctattgtgc gcttatacac
     7021 tcaaattaaa gataggttct aaataaatga gcgttttttg atagtctatt tcattaggta
     7081 atatatattt gtaacaaatc aatcaacatg gaataaaatc atgctaccat ctatttcaat
     7141 caacaatacc agcgcagctt acccagaatc catcaatgaa aataacaatg atgaaattaa
     7201 tggattagta caagagttca aaaacctttt taatggtaag gaaggaataa gcacctgtat
     7261 taaacatcta cttgagctta taaaaaacgc catacgagta aacgacgatc cttatagatc
     7321 taatattaat aattcctcag ttacttatat tgatattggc tccaatgata cagaccatat
     7381 tactattggt atcgacaacc aagaaccaat agaattacct gcgaactata aagacaaaga
     7441 actcgtccgt actatcatta atgacaacat agttgagaag actcatgata tcaataacaa
     7501 ggaaatgatc ttcagcgcat taaaagaaat atatgatgga gatcctggtt ttatcttcga
     7561 taagatatca cacaaactca gacatacggt aacggaattt gatgagagcg ggaaaagcga
     7621 accaactgac ttatttacct ggtacggtaa agataaaaaa ggcgactctc tcgctattgt
     7681 aattaaaaat aaaaacggaa atgattactt atctctcggt tactacgatc aggacgacta
     7741 ccacattcaa agaggaattc gtattaatgg tgatagtctc acccaatatt gtagtgaaaa
     7801 cgccaggagt gcttcagcgt ggtttgaaag cagtaaagct atcatggcag aatcatttgc
     7861 aactggttcc gatcatcagg ttgtaaacga gctcaacggg gaaagactga gagaacctaa
     7921 cgaagttttt aaacgtttag gtcgagcaat aagatataat tttcaagtgg acgatgcaaa
     7981 atttagacgc gataatgtaa aagaaataat ttctacttta ttcgctaaca aagttgacgt
     8041 tgaccatcct gaaaataaat acaaagactt taagaatctc gaagataaag tt
//
LOCUS       DBBBSB010000162         8070 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.164, whole genome shotgun sequence.
ACCESSION   DBBBSB010000162 DBBBSB010000000
VERSION     DBBBSB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.164"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..92
                     /locus_tag="ACIUS0_003736"
     CDS             <1..92
                     /locus_tag="ACIUS0_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221307.1"
                     /translation="GAVQRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(106..297)
                     /locus_tag="ACIUS0_003737"
                     /pseudo
     CDS             complement(106..297)
                     /locus_tag="ACIUS0_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            295..619
                     /locus_tag="ACIUS0_003738"
                     /pseudo
     CDS             295..619
                     /locus_tag="ACIUS0_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="ACIUS0_003739"
     CDS             complement(649..3561)
                     /locus_tag="ACIUS0_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGB0221308.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4044..4886
                     /locus_tag="ACIUS0_003740"
     CDS             4044..4886
                     /locus_tag="ACIUS0_003740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGB0221309.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="ACIUS0_003741"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="ACIUS0_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HGB0221310.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGAGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="ACIUS0_003742"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="ACIUS0_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HGB0221311.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..7941
                     /gene="lpxM"
                     /locus_tag="ACIUS0_003743"
                     /gene_synonym="msbB"
     CDS             6997..7941
                     /gene="lpxM"
                     /locus_tag="ACIUS0_003743"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGB0221312.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2170 a         1810 c         1926 g         2164 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 acccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtgggggct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7321 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaattcg gcacaataac
     7381 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7441 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7501 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7561 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7621 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7681 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7741 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7801 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7861 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7921 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7981 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     8041 gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DBBBSB010000163         7743 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.165, whole genome shotgun sequence.
ACCESSION   DBBBSB010000163 DBBBSB010000000
VERSION     DBBBSB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.165"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACIUS0_003744"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="ACIUS0_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HGB0221313.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="ACIUS0_003745"
     CDS             1102..2562
                     /locus_tag="ACIUS0_003745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGB0221314.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACIUS0_003746"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="ACIUS0_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGB0221315.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACIUS0_003747"
                     /gene_synonym="ydfH"
     CDS             complement(2979..3665)
                     /gene="rspR"
                     /locus_tag="ACIUS0_003747"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGB0221316.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACIUS0_003748"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="ACIUS0_003748"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGB0221317.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="ACIUS0_003749"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="ACIUS0_003749"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGB0221318.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="ACIUS0_003750"
     CDS             complement(6727..7245)
                     /locus_tag="ACIUS0_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGB0221319.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACIUS0_003751"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="ACIUS0_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGB0221320.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1940 a         1812 c         1956 g         2035 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DBBBSB010000164         7721 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.166, whole genome shotgun sequence.
ACCESSION   DBBBSB010000164 DBBBSB010000000
VERSION     DBBBSB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.166"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..832)
                     /locus_tag="ACIUS0_003752"
     CDS             complement(<1..832)
                     /locus_tag="ACIUS0_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221321.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACIUS0_003753"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACIUS0_003753"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGB0221322.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARVKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACIUS0_003754"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACIUS0_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGB0221323.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACIUS0_003755"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACIUS0_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGB0221324.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACIUS0_003756"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACIUS0_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGB0221325.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACIUS0_003757"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACIUS0_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGB0221326.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACIUS0_003758"
     CDS             complement(5530..6513)
                     /locus_tag="ACIUS0_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGB0221327.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACIUS0_003759"
     CDS             complement(6559..7383)
                     /locus_tag="ACIUS0_003759"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGB0221328.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACIUS0_003760"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACIUS0_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGB0221329.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2011 c         1812 g         1831 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggtgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DBBBSB010000165         7680 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.167, whole genome shotgun sequence.
ACCESSION   DBBBSB010000165 DBBBSB010000000
VERSION     DBBBSB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.167"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..182)
                     /locus_tag="ACIUS0_003761"
     CDS             complement(<1..182)
                     /locus_tag="ACIUS0_003761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221330.1"
                     /translation="MTKNINFSRFALSPYITENSGDLLASIFMPHLPSGVASDSQYVR
                     VEKSDYSHWVIYRHKQ"
     gene            complement(336..1295)
                     /gene="nrdF"
                     /locus_tag="ACIUS0_003762"
     CDS             complement(336..1295)
                     /gene="nrdF"
                     /locus_tag="ACIUS0_003762"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGB0221331.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1305..3449)
                     /gene="nrdE"
                     /locus_tag="ACIUS0_003763"
     CDS             complement(1305..3449)
                     /gene="nrdE"
                     /locus_tag="ACIUS0_003763"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGB0221332.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3422..3832)
                     /gene="nrdI"
                     /locus_tag="ACIUS0_003764"
     CDS             complement(3422..3832)
                     /gene="nrdI"
                     /locus_tag="ACIUS0_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGB0221333.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3829..4074)
                     /gene="nrdH"
                     /locus_tag="ACIUS0_003765"
     CDS             complement(3829..4074)
                     /gene="nrdH"
                     /locus_tag="ACIUS0_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGB0221334.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4322..4651)
                     /locus_tag="ACIUS0_003766"
                     /pseudo
     CDS             complement(4322..4651)
                     /locus_tag="ACIUS0_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            4803..5147
                     /locus_tag="ACIUS0_003767"
     CDS             4803..5147
                     /locus_tag="ACIUS0_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGB0221335.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5184..5633)
                     /gene="alaE"
                     /locus_tag="ACIUS0_003768"
     CDS             complement(5184..5633)
                     /gene="alaE"
                     /locus_tag="ACIUS0_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGB0221336.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            6301..6705
                     /gene="stpA"
                     /locus_tag="ACIUS0_003769"
     CDS             6301..6705
                     /gene="stpA"
                     /locus_tag="ACIUS0_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGB0221337.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(6752..7276)
                     /gene="ygaP"
                     /locus_tag="ACIUS0_003770"
     CDS             complement(6752..7276)
                     /gene="ygaP"
                     /locus_tag="ACIUS0_003770"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGB0221338.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(7286..7585)
                     /locus_tag="ACIUS0_003771"
     CDS             complement(7286..7585)
                     /locus_tag="ACIUS0_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGB0221339.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         2009 a         1828 c         1864 g         1979 t
ORIGIN      
        1 atttgtttat gtcgatatat tacccaatgg gaatagtcac ttttttctac cctaacatac
       61 tgagaatctg aggcaacccc tgagggcaaa tgtggcatga atattgatgc gagcaaatcg
      121 ccggaatttt ccgtgatata agggctgaga gcaaatcgag aaaagttgat attttttgtc
      181 atagatagcg aaaaaacgcc tgaatattgt agtttcaggc gaataatgca acagagtatc
      241 agtgtagatc actacagaat atttgcgtga taaaaagcta tttggcggga attatttccc
      301 tgctgcgggt agtgatattt ttgaaaataa caccctcaga aattccagtc ttcatcttct
      361 gtttcaaccg ctttccccat cacataagag gagcctgaac cggaaaagaa atcgtgattt
      421 tcatcggcat tcggcgaaag cgcggcgagg attgccggat tcacttccgc catttctgcg
      481 ggaaataacg gttcgtagcc cagattcatc aaagccttat tggtgttgta acagagaaac
      541 gctttcacat cgtcagccca cggggtttcg gcgtacagct catcggtgta ttgcaactcg
      601 ttgtcgtaga gttccagcaa caaatcgaag gcgaaactct tcaactcttc acgttgtccc
      661 agagatatct tttccatgtt tttctgatat ttatagccta tgtagtaacc gtggactgct
      721 tcatcgcgga taatcagacg gatcaggtcc gcggtattgg tcagctttcc gcggctggaa
      781 aaatacatcg gcagccagaa accggaatag aacaaaaaag attcaagaaa cacactggcg
      841 attttctttt tcagcggatc atcaccgcga taatgttgct gaataatctg agcttttcgc
      901 tgcaacggtg cgttttcttc actccaggcg taggcggcat cgacatcttt ggtctggcac
      961 agcgtcgaga aaatcgaact gtaagagcgg gcatgaaccg cttccataaa gctgatattc
     1021 gataataccg cctcttcatg aggcgtgagc gcatcgggca tcagcgaagg cgcgccgata
     1081 acattttgca gcgtgtcgag cagcgtcagg ccagtaaaaa cgcgcatcgt cagttgttgt
     1141 tctacgacag ttaatgtctg ccaggcggga atatcgttcg acagcggcac cttttctggt
     1201 agccagaaat tgctggtcag gcgattccac acctccagat ctttatcgtc agatatcttg
     1261 ttccagttga tggcgctgat acgtgagagt ttcatagata ttccttaaag tgcacaggag
     1321 acgcagcctt caatttcagt gccttccagc gccatctgac gcaggcggat gtaatagagc
     1381 gttttgatac ctttgcgcca ggcgtaaatc tgcgctttgt tgatatcgcg agtggtggcg
     1441 gtatcgggga aaaacagcgt cagcgacagc ccctgatcga catggcgagt cgcttccgcg
     1501 taggtgtcga tgatcttttc tgcgccaatt tcgtaagcgt cctgatacag cgccagattt
     1561 tcgttagtca taaacggggc agggtagtaa acgcgtcctg ttttgccctc tttgcgtatc
     1621 tctactttcg ccacaatcgg atgaatactc gacgtagcat ggttgatata agagatagac
     1681 ccggttggcg gcaccgcctg gagattctgg ttgtatatgc cgtagcgcat cacgtcgtcg
     1741 cgcagttgcg cccacatctc acgggtaggt aacgtaatac agctgcgggc aaacagttcg
     1801 ccaactttcg ccgttttcgg ctgccagtta ccttgcagat attggctgaa atattcacca
     1861 ctggcatagc gtgactgttt gaacccggcg aaggtttcac cgcgttcgcg cgccagcaac
     1921 atcgaggtac gcagtgcgtg ccaggtgatg gtatagaaat agagattggt gaaatccagg
     1981 gcttccggcg aaccataagc gatgccttcc ctcgccagat aaccatgtaa attcatctgc
     2041 cccagtccga tggcgtgcga ggcggcattt ccggcttcga tggacggcac gctgcggata
     2101 tgactcatat ctgacaccgc cgttaaaccg cgcacggcag tctctaccgt gcgggaaaaa
     2161 tcgggggaat ccatggtgtg cgcaatattc aacgaaccta aattgcagga aatatcatgg
     2221 cctgtgcggg catagtcgag attctcgtca tactctgagg cgctgttaac ctgcaaaatt
     2281 tctgagcaga gattactcat atttatgcgc ccggcgatag ggttagcacg gtttactgtg
     2341 tcttcataca tgatgtaggg atagccggac tcaaactgga tttctgccag tcgctggaag
     2401 aaatcacggg cgttgaggta ttttttgcgg atgcgttcat cggcaaccag ttcgtcatag
     2461 tgttcgctaa tggcgatatc ggcaaacggc ttgccataaa ctcgctcaac gtcatagggc
     2521 gaaaacagcg ccatctgcgc attctctttt gccagatgga aagtgatatc cgggatcacc
     2581 acgcccagcg acagtgtttt aatgcggatt ttttcgtcgg cattttcccg tttcgtgtcg
     2641 agaaaacgca gaatatcggg atgatgagca tgtaaataga ctgcacctgc cccctgacgc
     2701 gcgccgagtt ggttggcata ggaaaatgcg tcttccaaca ttttcatcac cggaattacg
     2761 ccagaagatt gattttcaat acgtttaatt ggcgcgcccg cttcccgcag attcgacagc
     2821 aaaaatgcta cgccgccgcc gcgtttcgac agctgcagtg cggaatttac cgcccgacca
     2881 atcgactcca tattgtcttc aatacgcagc aaaaaacagg aaaccagttc gccgcgctgc
     2941 tgcttaccgc agttgaggaa tgttggcgtg gctggctgaa agcgtcctga cagcatttca
     3001 tcggtcagtt gcaacgccag cgtctcatcg ccttgtgcca gcgtcagcgc caccatcgtg
     3061 actcgatcgg caaaatcttc cagataacgt ttaccgtcga atgtcttcaa cgtatagctg
     3121 gtgtaaaact tccatgcccc gaggaatgtc tggaaacgaa aaccgctggt gtgtgcgtgg
     3181 gcaaacagcg taatgacaaa gtcgcgagag tagcgattaa gaacgctttc atcatagtaa
     3241 ccttcgttga ccagccagtt caggcgctgc tgctggctac tgaaggtcac actgttcgga
     3301 cgcacatgcg tcgcaataaa ggcgtcaacg gcctggcggt ctttatcgaa ctgaatgcga
     3361 cctgcgctat cgtaaagatt aagcatcgca ttcagcgcgt ggtaatccat cgtttcctgc
     3421 gtcaggcatt ctgcggttgt cgttgccaaa attcggttac tcctttacga acgttttcga
     3481 tatcgctttg cgtacccatg agttcaaaac ggtacagcca cggcacgccg catttccggg
     3541 caatcacatc tccggcgcgg ccatacgcct caccaaagtt gcgattacca gaagcaataa
     3601 cgccgcgaag caacgcccgg ttgtgctcgt cgtttaaaaa gcgaattacc tgtcgtggca
     3661 ccgcgccagc cgtaccgccg ccgccgtaag agggcacgat caggatgtaa ggctcgtcta
     3721 cctgaatccg ttcccgctca ttgagcggga tgcgcaccgc gggcagacct aaacgttcga
     3781 taaaacgctg cgtgttttcg gagctgctgg agaagtagac gagctggctc atgcactggc
     3841 cgcgtgtggc gctggatgca gacggttaat catgtccgga cggaaaccag accagctaag
     3901 atcgccagca atcactaccg gcaactgacg aaagccctga gcacgcaacg cttccgctgc
     3961 ttcaggaacg cgatcgacat taatcatttc aaaatcaaag ccccggtttt ccatcgcccg
     4021 tttggtggcg tggcactgaa cgcaatcgtt acgagtataa atagttatgc gcatgattcg
     4081 tatttccgtt taaaatgaag atacggcgcg atgatacgcg tcgggttgtc tctctgttga
     4141 tacagagata ctagatgtag ttgaaaaaag attcaactac acaatatata gcaaatagtg
     4201 atcgaaatta ccctggatat gagcgtgacg ggggaggggg atttttgtga ttcaccaggc
     4261 aaaaagaaac cccgaagaca ggcttcgggg tcaaagacgc gtatttatta tcatttttgc
     4321 actacgattt gcgcatgctt aacagtgcgc cgataaaaat acctaccgca gctgctgtac
     4381 ccacgctaca ccagggtctt tcacgaacaa aagaatccgc tcagccaacg gcatcgcgcg
     4441 cggcttgctg gacgcgagta cgaccatgca ttcgcgccct ggtttctttc agcaacgcct
     4501 gagctttact gcgtgcggct tcagcttccc ctttggcgtc gctgccccag gacttcaata
     4561 cagattcgag gctgtcagct aattgattga catcattctg gatatcctgc acgccatcat
     4621 caacgtcgtt gcggttcggt cggttaaaca tatgatcccc cattagaatt cgatgtaatt
     4681 ttcagtgtag accataattt tcttaactga ggttcatcgc ggctttatgg acttttctga
     4741 aagcgttgtg aatgcgcaat caggtaaggt aggcccgcgg ttgaaaataa cgaggatcaa
     4801 atatgtattt acgaccagac gaggtggcgc gcgtacttga aaaagtcggt tttactgtcg
     4861 atgtggtaac ccagaaagcg tatggttacc gccgtgggga aaattatgtc tatgttaatc
     4921 gcgaagcgcg aatgggacgt accgctctgg taattcatcc aacattaaaa gaacgcagtt
     4981 caacactggc tgaacccgct tccgatatta aaacttgcga tcattaccag caatttccgc
     5041 tctatttagc aggcgagcga cacgagcatt acggtatccc gcatggcttt agttcgcgtg
     5101 ttgcgcttga acgttatttg aatggtttat ttggcgaagc cagttaaaga cgcgactggc
     5161 gatgccagtc gcgaaaagaa gagtcaggct tttacctgct ggtaacggct gactttaaac
     5221 agtcggcggc aataatcgag gaagtagcca taaaccgccc ccatcaacat cgaaacaacg
     5281 atgtttgaac tgaccgccgc cataatctga tgccagtctg cgcccaccac taacaagatc
     5341 gccacataca ccggtgactg gaacgtcaca taagccagga tatcagccag attttttatc
     5401 cagcccgacg ggctaacttt gcgtgccgcg cgcataaaca gatcgcggta cataccgtat
     5461 ggccatgcaa ttaagatgtt caccggaatc gctaccaatc tggaataaaa agactgttcg
     5521 aagctcattc cggagaggaa aacttcaata cacatgttca cgacagaaca gtaaacaacc
     5581 atcgcgaacg tatctgcaac tgcatgacgc aagcgtgact gcggtgagaa catgctaatg
     5641 ctccttaata aaagcgtaat gggatgttaa tggagatgaa agcagtggat ctcattgcca
     5701 tctgcatttt tgcatagcgt atatcgctgt ctatgactaa tcaattagtg aaaaattttt
     5761 atttagttag tttttgtaga taaaatattc cgtaacggct cgttttttgt acattgtgga
     5821 tttttacatg ccaatatgat ttattttcaa ttaaatcaat tagatatgat cttgccttta
     5881 atttcactaa tgaaagtttg ggggggaggt aacgctataa gcgtagagaa ttcggtgttt
     5941 gtcatgaccg ggtcatttac agcatttttg atgccagaaa atgtacatta acagcagaaa
     6001 gcaccagtga ggggtgtttt cttttattct gttatattgc atttttattc acttcggttt
     6061 tgtcaggctt gcggagttag cgagcagaga gcgccactct gttcaccaat aacctaaatt
     6121 actcatcaaa atggctaata tataatcttg aaattattct gagagtttca gaaaataaca
     6181 gctgaaataa tctcgcgcag gactgtaaat agattaaatt ttgtggaaat ataataagtg
     6241 atcgcttaca ctacgcgacg aaatactttt ttgttttggc gttaaaaggt tttctttatt
     6301 atgtccgtaa tgttacaaag tttaaataac attcgcaccc tccgtgcgat ggctcgcgaa
     6361 ttctccattg acgttcttga agaaatgctc gaaaaattca gggttgtcac taaagaaaga
     6421 cgtgaagaag aagaacagca gcagcgtgaa ctggctgagc gtcaggaaaa aattagcacc
     6481 tggctggagc tgatgaaagc tgacggaatt aacccggaag agttattggg taatagctct
     6541 gctgctgcac cacgtgctgg taaaaaacgc cagccgcgtc cggcgaaata taaattcact
     6601 gatgttaacg gtgaaactaa aacctggacc ggtcagggcc gtacaccgaa gccaattgct
     6661 caggcgctgg cagaaggtaa atctctcgac gatttcctga tctaattact gaggccggat
     6721 gtcgctgcta gggcgcgtcc ggcgtcgctt ctcaagctcg ttggttccac ggcatcttat
     6781 ctaacaacct tgccattcca caaaaaccgc tgattcctgc aaacagtaac ccggcaccaa
     6841 caaagccact taataagaag aaaccgctat ttacggtata acccagtaca acgccgttta
     6901 atattaaacc acccgcagcg atctgcacct ggcgcattaa cggcaagggt tgagatttat
     6961 ttaccgctac tggcaatcca gctcttttcc agccatcaat cccatcttcg agtaaaaata
     7021 tttctgcggg ggcggcaatc gctgctaatt tatcggcgtt attactggtg cgtttacctg
     7081 cctggcagtg gaatataatt tgctcgcgac gtaatttagg tggaagacct gactgttcca
     7141 gcacggataa tggagccaga tctgcttcag gaatatgttc acgaagatat tcatcagcgt
     7201 cacgaatatc gattaacttt gcgccgcgtg cgattaattc ttgtgcatca tgcggcgaaa
     7261 tggttgtcaa agccatagtg actccttacg gacaatagac atttttcagg gtggcgataa
     7321 tggcatttac cgcctcattt ttaatggaat atagaatgcg ttgggcatcc cgttggctgt
     7381 tgataagccc ttcgtcccgc atacgagcga gatgctgtga tgtcgcagag gcgctcagtc
     7441 cggtaatgcg cgtcagctct cccgcgctgg tgccggggga accgctaagc atgcacagaa
     7501 tcagcaaccg tttagggtgg ctcattgctt tcaataaggc cgctgcctgt tcggtactgg
     7561 cctgtaattg cgcgagttca gtcatattta tttaagtatt ttctaaatta agtaaactct
     7621 aaactaaaaa tgcaacatat accagcctca gcagcgtaaa tgagagtaaa agcgtaagct
//
LOCUS       DBBBSB010000166         7633 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.168, whole genome shotgun sequence.
ACCESSION   DBBBSB010000166 DBBBSB010000000
VERSION     DBBBSB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.168"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(121..1592)
                     /gene="yeeO"
                     /locus_tag="ACIUS0_003772"
                     /pseudo
     CDS             complement(121..1592)
                     /gene="yeeO"
                     /locus_tag="ACIUS0_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1753..1828
                     /locus_tag="ACIUS0_003773"
     tRNA            1753..1828
                     /locus_tag="ACIUS0_003773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Asn,seq:gtt)
     gene            complement(1866..2816)
                     /gene="cbl"
                     /locus_tag="ACIUS0_003774"
     CDS             complement(1866..2816)
                     /gene="cbl"
                     /locus_tag="ACIUS0_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGB0221340.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2918..3835)
                     /gene="nac"
                     /locus_tag="ACIUS0_003775"
     CDS             complement(2918..3835)
                     /gene="nac"
                     /locus_tag="ACIUS0_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGB0221341.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4163..4238
                     /locus_tag="ACIUS0_003776"
     tRNA            4163..4238
                     /locus_tag="ACIUS0_003776"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4196..4198,aa:Asn,seq:gtt)
     gene            complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="ACIUS0_003777"
     CDS             complement(4294..5229)
                     /gene="ldtA"
                     /locus_tag="ACIUS0_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGB0221342.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="ACIUS0_003778"
     CDS             complement(5291..6370)
                     /gene="cobT"
                     /locus_tag="ACIUS0_003778"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGB0221343.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="ACIUS0_003779"
     CDS             complement(6382..7125)
                     /gene="cobS"
                     /locus_tag="ACIUS0_003779"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGB0221344.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFIFGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7122..7631)
                     /gene="cobU"
                     /locus_tag="ACIUS0_003780"
     CDS             complement(7122..7631)
                     /gene="cobU"
                     /locus_tag="ACIUS0_003780"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGB0221345.1"
                     /translation="MVVSAIASTPHSSQVLYIATSQILDDEMAARIEHHRQSRPEHWR
                     TVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINA
                     EIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLV
                     VSGIGVKIK"
BASE COUNT         2109 a         2061 c         1658 g         1805 t
ORIGIN      
        1 accgactgaa ctacagagga atcgtgagaa cgaggcgaat attagcgatg cccacccaca
       61 atgtcaaagc ctgtttttta aatttgaaat cgtttgctga aataatctgc attttgtcgt
      121 ttattccgac acaactggct ttttttcaca cttttgcggc tcggatcgag ggtatttcca
      181 cagccaacgt ccagtaacca ttcgccagta aaacagcacg gcccgcacag cccagtcggc
      241 aaacattccc atccagacac caaccacacc ccagccaagc atgattccct gcacataacc
      301 gactacaacc cggcaacccc acatgctcaa catagaaacc cacatggcgt aacgggcatc
      361 acgagcaccc ttaaatccag cgggtagcac ccatgaggcg gaccaaatag gcataaataa
      421 agcatttagc taaatcagaa tcacaacgac gtgtttaacc tgtggatcct gggtgtaaaa
      481 cgatgccata accccggcaa agggagccgt tagccaggcg atggccgtta atccaagagt
      541 ggacagccag aacacatgcc gcaactgaat ctctgcttgc gctatctgcc ctacccccaa
      601 ccttcggcct gtaatgatcg tagaagcaga accgagcgca cttccgggta agttgataag
      661 agccgcaatt gagaacgcga taaaatttcc ggcaataacg ctggtcccca tcccggcaac
      721 gaacatttgg gttaataacc gaccactggt aaataacact gattcgacac tcgctggaat
      781 accaatcccc atgacttccc agataatgct aaaattcagc ggtttaaaat agctctttaa
      841 cgaaatcctt agcgcaggat taaaaccaat cgccagcacc cacaaaattg caactgcgcc
      901 aatataacga gaaatggtta aacccaaccc agccccgacc aggagaaaag gccgtaaatc
      961 aatatgccgc taataataat attaagaata ttcaggctac cgttaatcag tagcggtatt
     1021 ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc agcggctgga
     1081 taactgagta cggtcagctc cagataagtc aacgccagtg ctttaacttc tgtcgtggca
     1141 tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaattagcgt tgcgaacagt
     1201 acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga
     1261 tcccgcttac cgagactaaa tgctacaacg acagtagtac caagatcgat agctgcaaaa
     1321 aaagccataa tgaccatatt gaagctgtcc gccaacccca cgccggccat cgcatctttt
     1381 cccagccaac tgaccagaaa agtgctcagg acccccatca acaggacaca ggcgttctcc
     1441 atgaagatag gaacagcaag cggggttatc tcgcgctaga acaacacttt gtagctcttg
     1501 cgtttagagt gccagcgagt gccgtgaaca acctggcgta aagcagagga gatattcaaa
     1561 gccgacctta attgcagaaa gtgaaaccac atttcaaatg atgagggaga atcagcaaag
     1621 ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga tagaaacggc
     1681 aaacagttgg gaaatttaaa aatcgggttt gacaaaatat ttttcgccgt taagatgtgc
     1741 ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     1801 actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt atagcgggct
     1861 ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg gcgcttgata
     1921 tcctcaactg acagtcctgc attacaaagt tccagaaagc gccaaacata gttacgctga
     1981 agttgtcctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg ccgcgtatcc
     2041 aggcggatta aattctcttc ctcttgttcg ccgctggatt gttcggcaac taatccgatc
     2101 ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgtgcact taatacaata
     2161 tctgccggca aacctttgcg ggtaaatgcg tcatcagtgc gtgagcgccc cgtaattccc
     2221 tgtcggtaag taattaacgg ccacttcgct attgattcca gcgtcaatgg tgtaatttgc
     2281 gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc aacaaaacca tgggaaggcg
     2341 acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc ttcgccattt
     2401 tgcaacaacg tcgcaatttc ctgtggcgaa ccctggatta gctcgagccg aacctccggg
     2461 aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg agtatgcgtc
     2521 gttgcaatag tgagaacacc agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca
     2581 ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc cggttcagtc
     2641 atccccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaaattc atcctccagt
     2701 tcccgaatat gacggctaac gcctgactgt gaggtaaaaa gcatattcgc aacctctgtc
     2761 aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa attcacggta
     2821 aactccgggc agttcagata tcccgttatt gttaaagtct aatgcccggc ataacaaata
     2881 ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat tgccactgcc
     2941 ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact gcctgtgcct
     3001 gtggtgacaa gtttgctctg gcgggtaaat ttaaggacaa agagagactc attgaaggag
     3061 tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgca gccgattcag
     3121 gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca atagactcaa
     3181 tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa agcctcatca acacgaagtc
     3241 taacagcgct gtaatcactg gggagaaaaa ggttcatttg cgcaatagca ttaacatcaa
     3301 cgttttgccc cgggcaatct tgagttccta ccaggaaaag atcttctttc agcaaagcct
     3361 gactggatac cccagccaca ggggaatgct cataaatcac cgccatatcg agttggtgat
     3421 ttatcagttt ttcgttaagc actgcaccac tattttcatg aagatagata acgacctccg
     3481 ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca gcggttcctg
     3541 gcgcaaagcc aatcgagact tgccccgata atgactgacc aacgttatgc accgccagtt
     3601 gggcctgctc acactgacgt aaaatggccc gtgcatgggt atagagaatt tttccggcgt
     3661 ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagctg ttgatttaac tcaccttcca
     3721 gtatggcaac ctgttggctg agcgctggtt gtgcgatatg caatacttca gcagcctggg
     3781 tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag ttcatgttgc
     3841 ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagta tgcaagatgc
     3901 ctgccagttc tattttggcg ttgatgcgca agctaaccaa ctgtcaaata agagattatg
     3961 atagattcgt cattggccct ttttatcagc agtcgcattc acctgcccta taaaaggagg
     4021 gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt tgttgaaatt
     4081 ggcagcaaac aaacaaaaaa aatgcatttc accctttgac atcaccatgc actgccatta
     4141 atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     4201 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcaaaaaag cctgctttcg
     4261 agcaggcttt ttactttcta attaccaacg ctcttaaaac atctgtcttg aaccagaact
     4321 aatttgcaca ggcattcccg atcgatgttg caacgcagca tttgcgcgat ttacatcaac
     4381 ttcttgcccg ttgataaacg cccgcaaaga tggggttacc ggcaatggca cttttcggtc
     4441 agactcatat tctgcacgat tgcgcgacag cggctcatga acttccagcc actttgaacc
     4501 atccggttcc gttgtgtatt tcactggctg atcaataatt tgtacacgag tgcctacagg
     4561 aacattatca aacagatatt tgatatcgtc attacgcaga cgaatacagc cctgacttac
     4621 acggagccca ataccaaaat tggcattggt accgtggatg gcatacaacc tgccaatata
     4681 aatcgcgtac agccccatgg ggttatcggg ccccgcagga acaaatgcgg gcaaactctc
     4741 ccctcgtttc gcatattcgc gccggatgtt cggcgttggc gtccaggttg gtgcttcttg
     4801 tttacgttca acggtagtca cccagttacg cggggtttct cgcccagcct ggccgatacc
     4861 aataggaaag acttccactg tattactgtc tggcggatag taataaagac gcatttcagc
     4921 gacgttaacc acaatccctt tacgaacagt ggcgggcaaa atcagttgct gcggaatggt
     4981 gagttgtgag ccaggcttcg gcaaaaaaac atcagcgccc ggattcgctt ccagcatatt
     5041 acttaaccct tgcccgtatt gtgcggcaaa agtctccagc ggctgagtat tgtgatcagg
     5101 aacagttaca gtaagcgact gccccactaa acggctaccc tctggaggta atggataagt
     5161 taccgccagg ctattttggc tggcaaaaag cagagcaaat gagcaaagaa tatttacacg
     5221 acgcatcatg tccctttcct aagtcgcgaa tactatccga taagtatagc ttttatcaga
     5281 cttttcgttt ttagctgttc aaatcagaag tcgtattccc cggtagaaca atattactgg
     5341 cagcaagttc gcccatgttg ttgtatatcg cacaagcagc ttcgatgatg gacatcgcca
     5401 gagctgcacc actcccctca cctaaacgca tatccatatt gagataaggc tccagcccca
     5461 aatgcgagag cgctatacgc gcgccttttt ctgccgacaa gtgagaagga atgagatacg
     5521 gtttgattgc aggagacatc tggcaggctg cgagcgcagc agcataagaa agaaatccat
     5581 ccagcaacac gggtaaaccg caggaagcag cacctaacat cactccagct attccgacca
     5641 aatcaaatcc acccactttt gccaggacat caacaccatc ctgaggattt ggttgattca
     5701 acgtaatcgc ccgacgcaca acatcaattt tattagccag tttatctgtc ggcaggtttg
     5761 cgccaatccc aaccacttct tcaggatccc ggccagtgat tgtgctgact attgccgctg
     5821 ccggtgtcgt gtttgccatc cccagttcac ctacaccaaa cagcgtgaca ccgtttttcg
     5881 ccagctcccg cgtataacat atgacgtcca aaagcaactt ttcagcctga cggcgactca
     5941 ttgccggagc tgaagcaata ttgccgctac ctcgtgcgac acgcatgttg ataagcccgg
     6001 ggataggctc agcagtatca ataccaacat caattacgtg gacgttagcg cccgcttgtg
     6061 ctgccagcac acacacgccg gttgttccac gggtcatatt ttcagcctgt atggctgtca
     6121 cttcttttgg agaaatagcg accccttcct cccagacgcc gtgatcggca cacataacca
     6181 gtaccgcttt tttgcccaca tgcggtatgc cattcacccc cggcattcct gccagttgta
     6241 tggcaagcgc ctccagcttt cctaggctac caacaggttt gaataacccg tcaacatgcc
     6301 gttgtgcacg cgacatagcg gcagaattga tggctgggat cgtattcagt aaatcggcaa
     6361 ttatttgcat gtcacgtttc tttatagcag tgccagcagg aagaccagtt caccaagctc
     6421 tatggctgct cccagcgtat cgcccgtttg cccgcccagc gtacgtttga gaagctgacc
     6481 gaagatgaaa atcgccacca tcgtcaccac catagcagcc acaccatgca tacccagcaa
     6541 taatacggca gcaaaaatag cggctaagcc gagtgtgacg caggtttgtc gcccatcaat
     6601 tttgccaatg aacacattgc caagcccttc ttcccgcgca taacgatgac gatacatcaa
     6661 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     6721 gccacgcaga gccaactcgc ttaacaccag aatctttgcc agtaccacaa aaatcaatgc
     6781 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcgact
     6841 acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga attccccggt
     6901 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg
     6961 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt
     7021 aataataccg cgagaataat gttcgaaatc cagtccctgg gaccagcgac gcggtacagg
     7081 cagacgcgta ataaatgaga gcatcgccca aaataatttg ctcatttgat ttttactcca
     7141 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc
     7201 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     7261 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     7321 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     7381 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagcc
     7441 tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     7501 cgccagtgct ccgggcggct ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     7561 aggatttgcg aggtagcgat atacagaacc tgtgaagagt gaggtgtact ggcaatagcg
     7621 gacactacca ttt
//
LOCUS       DBBBSB010000167         7441 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.169, whole genome shotgun sequence.
ACCESSION   DBBBSB010000167 DBBBSB010000000
VERSION     DBBBSB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.169"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="ACIUS0_003781"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="ACIUS0_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="ACIUS0_003782"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="ACIUS0_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGB0221346.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="ACIUS0_003783"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="ACIUS0_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="ACIUS0_003784"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="ACIUS0_003784"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGB0221347.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="ACIUS0_003785"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="ACIUS0_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGB0221348.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACIUS0_003786"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="ACIUS0_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGB0221349.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="ACIUS0_003787"
     CDS             complement(6510..7202)
                     /locus_tag="ACIUS0_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGB0221350.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1880 a         1805 c         1963 g         1793 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 t
//
LOCUS       DBBBSB010000168         7432 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.170, whole genome shotgun sequence.
ACCESSION   DBBBSB010000168 DBBBSB010000000
VERSION     DBBBSB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.170"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="ACIUS0_003788"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="ACIUS0_003788"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGB0221351.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACIUS0_003789"
     CDS             complement(604..2001)
                     /gene="pcnB"
                     /locus_tag="ACIUS0_003789"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGB0221352.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACIUS0_003790"
     CDS             complement(2061..2957)
                     /gene="gluQRS"
                     /locus_tag="ACIUS0_003790"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGB0221353.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACIUS0_003791"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="ACIUS0_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGB0221354.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACIUS0_003792"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="ACIUS0_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGB0221355.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACIUS0_003793"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="ACIUS0_003793"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGB0221356.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="ACIUS0_003794"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="ACIUS0_003794"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGB0221357.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1699 a         1913 c         2078 g         1742 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tg
//
LOCUS       DBBBSB010000169         7379 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.171, whole genome shotgun sequence.
ACCESSION   DBBBSB010000169 DBBBSB010000000
VERSION     DBBBSB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.171"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1545
                     /locus_tag="ACIUS0_003795"
     CDS             <1..1545
                     /locus_tag="ACIUS0_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGB0221358.1"
                     /translation="FTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREV
                     VDTYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARF
                     GVYSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDN
                     FTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIG
                     VVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGV
                     KRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWW
                     KGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLT
                     WITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTV
                     ENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKK
                     ALSEAK"
     gene            1597..2754
                     /locus_tag="ACIUS0_003796"
     CDS             1597..2754
                     /locus_tag="ACIUS0_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGB0221359.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2720..2842)
                     /locus_tag="ACIUS0_003797"
     CDS             complement(2720..2842)
                     /locus_tag="ACIUS0_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221360.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3045..4730
                     /locus_tag="ACIUS0_003798"
                     /pseudo
     CDS             3045..4730
                     /locus_tag="ACIUS0_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4768..7128
                     /locus_tag="ACIUS0_003799"
     CDS             4768..7128
                     /locus_tag="ACIUS0_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGB0221361.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7185..>7379
                     /locus_tag="ACIUS0_003800"
     CDS             7185..>7379
                     /locus_tag="ACIUS0_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221362.1"
                     /translation="MRNLGFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2164 a         1615 c         1569 g         2031 t
ORIGIN      
        1 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact aaccatggat
       61 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
      121 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa
      181 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
      241 gtggcacacg gtgtttccgg cccctcccgt gccgcaataa tgaccggtcg agctcccgcc
      301 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
      361 ttgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
      421 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
      481 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
      541 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
      601 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
      661 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
      721 ctaccaaatg ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa
      781 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
      841 gaacaactga agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat
      901 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
      961 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     1021 ggtaattatg acaagctgat ttccgcaatg gatttctacc caacagctct tgatgcagcc
     1081 gatatcagca ttccaaaaga ccttaagctg gatggtgttc ccttgctgcc ctggttgcaa
     1141 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     1201 tttgatgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     1261 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     1321 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg
     1381 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     1441 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     1501 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     1561 gcggattttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     1621 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     1681 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgaa
     1741 gcgtctggca atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg
     1801 gattttttcc gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt
     1861 aatacattac aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa
     1921 catgaatttc tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga
     1981 cgcagtaatt caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa
     2041 tcatatcaag tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattatcct
     2101 cttgaggttt atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg
     2161 ctagaaaccg ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc
     2221 attgattttt ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa
     2281 tgggttaaaa acgatgtggg tgaaattttc attcgtcagt ttgaaagctt tgtcagccgt
     2341 tttttgggga atgggcatac aagttgtatt ttccaggagt cctgcaagga taatctggtt
     2401 gttgaaagta atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt
     2461 ggaaacatta ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa
     2521 aaacggattt cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggcggttgt
     2581 cctaagcatc gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat
     2641 aaaatccttt tttcaaccat ggtaccttat atgaacgcca tggttgagtt agctaagaac
     2701 agagtaccgc tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc
     2761 tctcttttaa ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa
     2821 ttcctgtcgg gtgtttcgtc atcatcatct tcccttgcga aaatcctggc ttacgtggta
     2881 tgcgtcaggg cattagcaat acccttgatt atcaatagca taaccataac cactgaaaat
     2941 taaaaatttc tatatatttc atattgttaa atgatgctta aataaatttc atcagtaaat
     3001 cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata accattccca
     3061 ttacgttttg tatgttgata gcaaagtatc tctgtctgct gaagcccttt tggctgcgta
     3121 agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg
     3181 ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga
     3241 aagaaaccga taagctttgg cagctcgttc tttggttccc tgccctgctg gggatctttg
     3301 tattaatatc cgcaaacaaa acatggttaa ttaaactact gactattcgc tggcgcgaat
     3361 ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac ttcacgcaga
     3421 tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct
     3481 tactgatcaa caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga
     3541 tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa
     3601 cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa
     3661 ctttatttac ccataaggtg ggtaaacgta ttcgtccgct taatgtggaa aaacaacgta
     3721 gcgaagcgac atttcgaact aatcttgtgc agcataataa gcaggcagaa ctgattgcgc
     3781 taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat accattaaag
     3841 agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa aatatttatt
     3901 cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga
     3961 tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg aacaatttaa
     4021 gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt
     4081 atgagttcca tcaactcact gaatagcgcc ctacgaataa gcctaaaaat tgccaacatg
     4141 cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata ttagagaact
     4201 tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa
     4261 aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttctt
     4321 ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaagcggc ttactgaaag
     4381 agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc
     4441 atcaggttgg tctgggaaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata
     4501 ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga
     4561 aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct atacgcttac
     4621 tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg
     4681 gtgtctggaa cctggctgat gatatttgtg acattagcac ggttttataa caacaataaa
     4741 agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta
     4801 tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgta tttttttgaa
     4861 gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt
     4921 gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac
     4981 cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct
     5041 gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt
     5101 agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt
     5161 agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat
     5221 gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc
     5281 aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgtttggac
     5341 tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg ttcttcagga
     5401 agtacctatt tctctccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggag
     5461 ctggctgata actttggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc
     5521 gcggattatg tttcgaatga cggcattgtc gccggtcggg cacagtataa aaacgttatc
     5581 gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc
     5641 ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc
     5701 gaaatgggta ataaatccta tggtctggca tgggatatgg atacgcaact cgcatgggcc
     5761 aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac
     5821 atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg tacccgtggc
     5881 ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg
     5941 caaaaattcg ccgtgggtga tgtttcgcat caaccctact tcggcgcgga atacatctat
     6001 tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgcagga
     6061 aagaaaacta accataccat ttaccataaa ggtaaaggca gcctgggaat tgacaactac
     6121 acgctgtata tggcggatca cattagctgg cggaatgtgt cgttaatgcc cggtgtgcgt
     6181 tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg
     6241 gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta ctatggcggg
     6301 aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt
     6361 aataaaaccc tgacgcgtta tcagaatttg aaaacgcctt ataacgatga actggcaatg
     6421 ggattgcagc aaaaaatcga taagaacgtt attgcacgcg caagtgaagc gcatgatcaa
     6481 atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca ccattactga atataacaac
     6541 gatggcaaaa ccaaaacgca ttcgtttaac ctcagttttg aactggccga acccctgcat
     6601 atccgccagg tagatattaa cccgcaaatt gtctttagct atatcaagag caagggcaac
     6661 ttgtcgttaa acaatggtta tgaggagagc aataccggtg ataaccaggt ggtttataac
     6721 ggtaatctgg tctcttacga tagcgttcca gtggcagatt ttaataaccc attaaagatc
     6781 tccttaaaca tggatttcac gcatcaaccg agcgggttag tgtgggcgaa tacgctggcc
     6841 tggcaagaag cgcgtaaagc tcgcattatc cttggtaaga caaatgcgca atacatcagc
     6901 gaatattcag attacaagca gtatgttgac gaaaaactgg atagcagcct gacctgggac
     6961 acccgcttgt cctggacgcc acaatttctg aaacaacaaa acctgacgat cagtgccgat
     7021 attctcaatg tactggatag caaaaccgct gttgatacaa cgaacaccgg tgtggcgacc
     7081 tacgccagtg gccgtacttt ctggcttgat gtcagcatga aattttaata gttcggattt
     7141 tacttaaccg ggtaaattcg cccggttttc gcatggagat aattatgaga aacctcggtt
     7201 tcttactgac gttagtggca actctgttgc tccccgggcg gctgattgcc gccgccttac
     7261 cgcaggatga aaagttaatt accgggcaac tggacaatga cttgcgatat atgatttatc
     7321 cgcatgctca tccaaaggat caggtaaatt tatggctgca aattcatacc ggttcattg
//
LOCUS       DBBBSB010000170         7191 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.172, whole genome shotgun sequence.
ACCESSION   DBBBSB010000170 DBBBSB010000000
VERSION     DBBBSB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.172"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACIUS0_003801"
                     /pseudo
     CDS             complement(12..705)
                     /gene="ospZ"
                     /locus_tag="ACIUS0_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            761..913
                     /locus_tag="ACIUS0_003802"
                     /pseudo
     CDS             761..913
                     /locus_tag="ACIUS0_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2294..3241
                     /gene="icsP"
                     /locus_tag="ACIUS0_003803"
     CDS             2294..3241
                     /gene="icsP"
                     /locus_tag="ACIUS0_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HGB0221363.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3803..4175)
                     /locus_tag="ACIUS0_003804"
                     /pseudo
     CDS             complement(3803..4175)
                     /locus_tag="ACIUS0_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4419..4538
                     /locus_tag="ACIUS0_003805"
                     /pseudo
     CDS             4419..4538
                     /locus_tag="ACIUS0_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4788..5654
                     /gene="ospB"
                     /locus_tag="ACIUS0_003806"
     CDS             4788..5654
                     /gene="ospB"
                     /locus_tag="ACIUS0_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HGB0221364.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5983..6723
                     /gene="phoN2"
                     /locus_tag="ACIUS0_003807"
     CDS             5983..6723
                     /gene="phoN2"
                     /locus_tag="ACIUS0_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HGB0221365.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            6796..6918
                     /locus_tag="ACIUS0_003808"
     CDS             6796..6918
                     /locus_tag="ACIUS0_003808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221366.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(7076..7191)
                     /locus_tag="ACIUS0_003809"
                     /pseudo
     CDS             complement(7076..7191)
                     /locus_tag="ACIUS0_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT         2250 a         1356 c         1386 g         2199 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aatctctggc gactcatctc ttaacacttg
      181 cataatctct ctttttatat catatagttc tataaaccga tttgcccgta gttctccttg
      241 ggaagaatca tttctgaata caacaattat atacaaaaaa tcactatttt ctttagagaa
      301 gatcttacaa ctaatctctg ggagctcaca taatgcatgt aagtcaggat ggtacaaact
      361 atccacagaa aattgcccca taggacgttt tccagatata actgtcaacc agcttccttc
      421 cggtgtactg gcttcggctc tggaaatagg ctgaaaatct tccggacatg gagaatacat
      481 tgtcttcata taattaagtc gtctggattt ctcattaata aactcctcta tttttaattg
      541 tgccatactt ctggttatac cagagatata cttcttgtca tatatgtttg gtgattcatt
      601 aaagtaacca tgttcaactc taggataaat atttctcact atatattcag tatttgcagt
      661 atttattgga aatacgttct taatattctt gatgggacta atcattttaa tctctatact
      721 caaatataaa cattaccatg aacaatataa ttgttcctaa gtggaatgtc tccacggaga
      781 acactttatc tatcgggaaa tagtgcgggc aacagtgttt aattatatcg agtgcaatta
      841 caatcgctgg cgacggcacc gttggtgtgg cggcctcagt ccggaacaat ttgaaaacca
      901 gaacctcgct taggcctgtg tccatattac gtgggtaggc tcacagagcc tcacttacga
      961 ctgcgtccat attatgtggg taggatcaat aaagacgttt tatatgtgtc tgcttgcagc
     1021 cagaaaattt ataccctgca ggtatcataa acatgcttga taacttaatt ggggctccct
     1081 cttcctttta tggtgtaatt gtttgattga atacttccgg ggatttcagt atgaaatgaa
     1141 gtatatttaa tatactttac aaaaagtttg tattagtgga ataaggacaa tagcttatcc
     1201 aaactgccaa gcctctttat tataagtaag atctggcact ggaatattct caaaaggact
     1261 aacatcatca ccacgacctt caaccaacca ttttattgca tttaaatccc agaatagttc
     1321 agtagtggtc aatttgggaa acgcacaata ttgtacggtt acgttatcgc aatagagtga
     1381 actctcgccc tatacggcgt tttcaaggat tagtccttaa tcggacaacc acattaaagc
     1441 ataataaata attaagcttt aataatatac tatagataca gttctattat aaaaaatact
     1501 attttaggtg atgcattaaa ttagtagaat atttattctt ttataaaagc accaatgaat
     1561 tgtccaagta aacatgagat aaaatgtgta gaggataact gagtttggtc actttaactg
     1621 tattagtcgc agtctgactc tcgactttaa aaggatggga attacctgta ctgatagtga
     1681 gaaagctttc cattgaaata agtcaaataa aagttgatgg cttcagtgct aaagtgaggt
     1741 gcccgctagc cgtattcact cgaatcggaa ttcaatgtat tgcttctgcc atttccataa
     1801 tatttcacgg gaaaaacaaa atctgaaaaa ctatacttaa gtgttgtatt gagtcagttg
     1861 agatacaaat acatatttat cagcacacgt tacgtcaaat acaggaggtt gtcatgttgg
     1921 tactgaaagg cacgttggct attgcagccg tgatgacaat ttattgtctt atcgttgttc
     1981 ttatggaacg cttttctgat tgatttcata ttggcgagag aacaagaagg tgagtagaat
     2041 tgtcgcggaa tcctgaaact atcagcctca ggtatgaaca ccaaaaggta ggtagagaaa
     2101 tctatggccc ccgttacttt attcgcacaa tgaggtattt ttttattttt acaaaagttc
     2161 aataaatatt ataataaaag atatgttctt ggtaacataa acaaatattt aattaaaata
     2221 atgcttggca ttgacgttta tcttataaag taagaagatc atggacattt caaccaaaaa
     2281 agtagagttc tcgatgaaat taaaattctt tgtacttgca ctttgtgtac ctgcgatctt
     2341 tactacacat gctaccacta actatccact tttcataccg gacaacatca gtacggatat
     2401 tagtctagga tctctgagtg gcaaaacaaa agaacgcgtt tatcatccca aggaaggggg
     2461 gagaaaaatt agtcaactgg actggaaata cagtaatgcg actattgtta gaggtggcat
     2521 cgattggaag ctaattccaa aagtgtcttt cggagtttcc ggttggacta ctttaggtaa
     2581 ccagaaagca agcatggttg ataaagactg gaacaattcc aatacccctc aggtatggac
     2641 cgaccagagc tggcatccca atacgcatct ccgtgatgct aacgaattcg agctgaatct
     2701 taaaggttgg ttattaaata atttggatta tcgacttgga ctaatagcag gttaccaaga
     2761 gagtcgttac agttttaatg caatgggagg gagttatatt tatagtgaaa acggggggag
     2821 caggaataaa aaaggggcac atcctagtgg tgaaagaaca ataggttaca aacagctctt
     2881 caaaatacct tatattggat taactgctaa ttaccgccat gagaattttg agtttggagc
     2941 agaactgaaa tatagtggtt gggttctttc atctgataca gataaacact atcagactga
     3001 gacaattttt aaagatgaaa taaaaaacca aaattactgc tctgttgctg cgaatattgg
     3061 atactatgtc acccccagtg caaaatttta tatagaaggc tccagaaatt acatttctaa
     3121 taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca ccattaaaaa
     3181 tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt atattttttg
     3241 aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca ttgtgagtca
     3301 tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc gggggaaaaa
     3361 cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc caggatttcc
     3421 ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca ttaccgaccc
     3481 tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg tatcaagtta
     3541 cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg atggaacagt
     3601 gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct atctttccaa
     3661 atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa ggaaacgcct
     3721 cttggtgtac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt atatgctgga
     3781 tcgttcgtgg gtggctttgc ccctatattt ccagacacct gttatcattg gcccgctgcc
     3841 gcagatattc ccgtggcgag cgataatcca gcgcactatg cggatgccat tcgttataat
     3901 gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtcaggtttg ggcatgatac
     3961 tgatgtagtc acactttatt gttttcacga agctctttgc tattctgtta ctctccggac
     4021 tccgcaccgc cgtgttcttc ggttcaacaa agtcccaaca tccgggtgaa ctggcgtgtt
     4081 tcattagctc ggtagcatga accattatcc gtcagtcact tcactggaga cgccggaagc
     4141 tcgttgccga agcggtgttc caccgctccc agcatgtcgc caacgggaga gcatctacgg
     4201 tctgattgaa ctgtttattc aaattgtcca ccgtctgtca gtacgctctg aacgtcggct
     4261 ggttaaaaca ctgctcgctg attttcagag ggttcacggt aaaacagcac ttttattccg
     4321 gattgcggaa gcggctctga ataatccgga tgggctggtt aaagaggttg tgtaccatct
     4381 gcatattgtt gaaccagatc gcagcggtaa gagaagttca tcatacttgg cgcagttacg
     4441 cgatgtttct gctagaggtg atgccgtaaa gaacggcaga acattaccgg aacaagatag
     4501 cggtctacct gcactggtat cagacccggg gcttccccgg atgatctcca cggtacttta
     4561 ggtactgtgt ctatattatg taggtaggat caattaactt tgtagttgaa attctttcat
     4621 tgaaataatg aataaaaaag acaaaccgca ttaaacaatg ccagaatata attgcatttt
     4681 tgttccgttt ttttaaatca tagtatacaa agaagcttgc atactatatg aaaatataac
     4741 accctttact atgatgttta taaaacaata tatggagtca tgtaggtatg aatttagatg
     4801 gtgttagacc atactgtaga atagtcaata aaaagaatga aagcatatca gatattgcat
     4861 ttgcacatat aataaaaagg gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg
     4921 tttttttagg agagaaaggt ttttgtgata gcaatgatgt tctatctatt atgggacaac
     4981 aaataccaag agtatttaag aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa
     5041 gtaaaaatga ttttctaaaa atggctgaat catggctacc acagagtgaa ccaatagtaa
     5101 taaataatga tgatgacgca ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa
     5161 taaaaacagt aaacgatact gattttaaag agtataataa ggtttatatt cttgggcacg
     5221 gtagtcctgg ttctcatcaa ttaggccttg gttcggaact tattgatgta caaacaatca
     5281 tttcaagaat gaaagactgt ggtattctaa atgtgaaaga tatccgtttt acttcatgcg
     5341 gctccgctga taaagtggct cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt
     5401 gtatccttaa ctctctgcct ttttttaagg aaaaagaatc tttgctagag cagataaaaa
     5461 aacaccttga aaacgatgag tcattgagtg atggtctaaa aatatccggc tatcatggat
     5521 atggagttca ctatggtcaa gagctttttc cctactcaca ttatcgttca acttcaattc
     5581 ctgctgatcc ggagcataca gtaaaaagaa gctctcagaa aaagactttt attattaata
     5641 aagaactgga ttagtataaa atttttaacc tatagttaat tgtttgcatt taagatgaag
     5701 ttattttatc tttagtagaa aagtgcttcc ctatgaggga aaatcacaaa tcatcataat
     5761 acagagacaa aacgatacga aaaaatagat aaaaaacatc attcttttac cacattattt
     5821 tccgtgaata tgaaaaataa tgttattact ttaatataag actatttttt gtttttccat
     5881 cactctgttc aaatttttcc gcatgacttg tgttttttgt aatacagctc gttttttaca
     5941 gctgaccaaa atcatcaatt aattatgcta aggaaataaa ttatgaaaac caaaaacttt
     6001 cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc tctgaaggca
     6061 gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc gccgcctccg
     6121 gcagagaatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg ccgctcgctc
     6181 cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa ttttggtctt
     6241 gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat cttatatcag
     6301 ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc caaagaatat
     6361 tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc tgataaagat
     6421 gagaaaatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg ttgggcagta
     6481 gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg acgtggatat
     6541 gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt agaggctggg
     6601 cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt taccaaaagc
     6661 cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga actgacccca
     6721 taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta ccctatatga
     6781 gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg attactggaa
     6841 aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc catcggaaac
     6901 gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg gggcggttcc
     6961 gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc tatgtcgatc
     7021 aatataacag aaatgacaac ttcagattca gggcacaacc taaatatatt ttaggtcacc
     7081 tctcaaatcg tttgcctgat accgctccat tttttaataa aaaaatcaat catttttgaa
     7141 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt t
//
LOCUS       DBBBSB010000171         7188 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.173, whole genome shotgun sequence.
ACCESSION   DBBBSB010000171 DBBBSB010000000
VERSION     DBBBSB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.173"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="ACIUS0_003810"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="ACIUS0_003810"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGB0221367.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACIUS0_003811"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="ACIUS0_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGB0221368.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACIUS0_003812"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="ACIUS0_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGB0221369.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="ACIUS0_003813"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="ACIUS0_003813"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGB0221370.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="ACIUS0_003814"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="ACIUS0_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="ACIUS0_003815"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="ACIUS0_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGB0221371.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAAFLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="ACIUS0_003816"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="ACIUS0_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGB0221372.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2032 a         1630 c         1506 g         2020 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tcttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttat
//
LOCUS       DBBBSB010000172         7183 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.174, whole genome shotgun sequence.
ACCESSION   DBBBSB010000172 DBBBSB010000000
VERSION     DBBBSB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.174"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(371..1198)
                     /locus_tag="ACIUS0_003817"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACIUS0_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACIUS0_003818"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACIUS0_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGB0221373.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACIUS0_003819"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACIUS0_003819"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGB0221374.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACIUS0_003820"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACIUS0_003820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGB0221375.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACIUS0_003821"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACIUS0_003821"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGB0221376.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACIUS0_003822"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACIUS0_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACIUS0_003823"
     CDS             7090..>7183
                     /locus_tag="ACIUS0_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HGB0221377.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DBBBSB010000173         7027 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.176, whole genome shotgun sequence.
ACCESSION   DBBBSB010000173 DBBBSB010000000
VERSION     DBBBSB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.176"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..4823
                     /gene="traI"
                     /locus_tag="ACIUS0_003824"
                     /pseudo
     CDS             1..4823
                     /gene="traI"
                     /locus_tag="ACIUS0_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            4843..5589
                     /gene="traX"
                     /locus_tag="ACIUS0_003825"
     CDS             4843..5589
                     /gene="traX"
                     /locus_tag="ACIUS0_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGB0221378.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5644..6204
                     /locus_tag="ACIUS0_003826"
     CDS             5644..6204
                     /locus_tag="ACIUS0_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGB0221379.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6335..6547
                     /locus_tag="ACIUS0_003827"
     CDS             6335..6547
                     /locus_tag="ACIUS0_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGB0221380.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            6791..>7027
                     /locus_tag="ACIUS0_003828"
     CDS             6791..>7027
                     /locus_tag="ACIUS0_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGB0221381.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1622 a         1763 c         2304 g         1338 t
ORIGIN      
        1 gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtgac taatgttacg
       61 cagtataacg gcgaatggaa gacactgagc agtgacaaag tggggaaaac ggggttcatt
      121 gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa actgaaagag
      181 caggttgagg cgctgggcta tgaaactgaa gtggtcggta agcacggtat gtgggaaatg
      241 ccgggtgtac cggtggaggc cttttccgga cgctcacaga ctatccggga ggccgtcggg
      301 gaagacgcct cgctgaaatc ccgggatgtg gcggcgctgg atacgcgtaa atccaaacag
      361 cacgtcgatc ctgaagtcag aatggccgag tggatgcaga cgctgaagga aaccgggttc
      421 gacatccggg catatcgtga cgcagcggaa caacgggcat atacccgcac gcagacaccc
      481 ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc cattgccgga
      541 ttaagtgaac gcaaagtgca gtttatgtat acggacctgc tggccaggac ggtcggcata
      601 ctgccgccgg aaaatggtgt gattgaacgg gcacgcgccg gtatcgatga ggccatcagc
      661 cgtgagcagc ttatccccct cgatcgtgag aaggggctgt tcacgttcgg gattcatatg
      721 ctcgatgagc tgtcagtccg ggcactcagt cgtgacatca tgaaacagaa ccgggtgacc
      781 gtacatccgg agaaaagtgt cccccggacg gccggttaca gcgatgccgt cagtgttctg
      841 gcacaggacc gtccgtcgct ggccattgtg tccgggcagg gcggtgcagc cgggcagcgt
      901 gagcgggtgg ctgaactggt catgatggcc cgggagcagg ggcgggaggt gcagattatc
      961 gctgctgacc gtcgctcgca gatgaacctg aagcaggatg aacggctgtc cggtgaactg
     1021 ataaccggac gtcgtcagtt gctggaagga atggccttca cgccgggcag taccgttatc
     1081 gtcgaccagg gcgaaaaact ctccctgaaa gagacgttaa ccctgctgga tggtgccgca
     1141 cgtcataacg tacaggtcct gataaccgac agcgggcagc gaaccggtac aggcagtgca
     1201 ctgatggcca tgaaggatgc cggggtgaac acatatcgct ggcagggggg agaacagcga
     1261 ccggccacca tcatcagtga accggaccgt aatgtcggct ggccggagat tttgcggcca
     1321 gcgtgaaagc cggagaagag agcgtggcac aggtcagcgg agtacgggaa caggccatac
     1381 tgacacaggc cattcgcagt gagctgaaaa cacagggcgt gctcggacac ccggaggtaa
     1441 ccatgaccgc cctgtcaccg gtctggctgg acagccggag ccgttatctg cgggatatgt
     1501 accgcccggg aatggtgatg gagcagtgga acccggagac acgcagtcat gaccgctatg
     1561 tgacagaacg tgtgacggcg cagagtcaca gcctgaccct gcggaatgcg cagggcgaaa
     1621 cgcaggtggt gcgtatttct tccctggaca gcagctggtc gctgttccgg ccggaaaaaa
     1681 tgccggtggc agacggtgag cgtctgaggg tgacagggaa aattcccgga ctccgcgtct
     1741 ccggcggtga ccgcctgcag gtggcatccg tcagtgaaga tgcgatgacg gttgttgtgc
     1801 cgggacgggc tgaaccggcc accctgcctg tgagcgattc accgttcact gcactgaagc
     1861 tggagaacgg ctgggtggaa acgcccgggc attccgtcag cgacagtgcg acggtttttg
     1921 cctccgtcac acagatggca atggacaatg ccaccctgaa tggtctggcc cgcagtggtc
     1981 gtgatgtccg gctgtattcc tcactggatg aaacccgtac tgcggaaaaa cttgcccgcc
     2041 atccctcctt tacggtggtt tctgagcaga taaaggcgcg tgccggtgag acattgctgg
     2101 aaaccgctat cagtctgcag aaagccgggc ttcacacgcc ggcacagcag gccattcatc
     2161 tggcccttcc tgtgctggaa agtaaaaacc tggccttcag catggtggac ctgctgacag
     2221 aggcgaagtc gtttgctgca gaaggaaccg gttttgctga cctgggaggg gaaatcaatg
     2281 cgcagataaa acggggtgat ttactgtatg tggatgtggc aaaaggctat ggcacaggcc
     2341 tgctggtttc ccgtgcgtcg tatgaggcag aaaagagcat tcttcgccat attctcgaag
     2401 gtaaggaggc ggtcacgccg ctgatggaga gagtacctgg cgaactcatg gagaaactga
     2461 catcaggaca gcgcgccgcc acccgtatga tactggaaac gtctgaccgt ttcacggtgg
     2521 tgcagggcta tgccggtgtg ggtaagacca cacagttccg ggcggtgatg tcagccgtga
     2581 acatgctgcc ggagagtgaa cgtccccgtg tcgtggggct ggggcccaca caccgtgcgg
     2641 tcggggagat gcgcagcgcc ggcgtggatg cacagacact ggcgtccttt ctgcatgaca
     2701 cgcagctgca gcagcgcagc ggagaaactc cggatttcag caacacgctg ttccttctcg
     2761 atgagagctc tatggtgggc aataccgaca tggcacgggc atacgccctg attgcggccg
     2821 gtggcggtcg ggctgtggcc agcggtgaca cggaccagct gcaggccatc gcgcccggtc
     2881 agcctttccg tctccagcag acgcgcagtg ctgccgatgt ggtcatcatg aaggagattg
     2941 tgcgtcagac gccggaactg cgggaggcgg tatacagcct gattaaccgg gatgtggaaa
     3001 gggcgctgtc cgggcttgag cgtgtgaaac cgtctcaggt gccacgtctg gagggcgcat
     3061 gggcaccgga gcactccgtg acggagttca gtcacagcca ggaagcgaaa ctggcagaag
     3121 cgcagcagaa ggcgatgctg aaaggcgagg cttttccgga tgtccccatg acactgtatg
     3181 aagccattgt ccgcgactat accggcagga caccggaagc acgggagcag acgctgattg
     3241 tcacgcacct gaatgaggac cggcgcgtac tgaacagcat gattcatgat gcacgggaaa
     3301 aggccggaga actggggaag gtgcaggtca tggtgcctgt cctgaacaca gcgaatatac
     3361 gcgacgggga gctgcgtcgt ctctccacct gggagaataa cccggatgcc cttgccctgg
     3421 tggataatgt gtatcaccgg attgccggta tcagtaagga tgacgggctg ataaccctgc
     3481 aggatgcgga aggtaacacg cggctgattt cgccccggga ggcggtggct gaaggcgtca
     3541 cgctgtacac cccggacacc atccgggtgg ggaccggtga caggatacgc ttcacgaaga
     3601 gtgaccggga gcgcggttat gtggccaaca gcgtctggac ggtgacagcg gtttccggtg
     3661 acagtgtcac actttctgac ggtcagcaga cccgtgtgat tcgccccggt caggagcggg
     3721 cagagcaaca tattgacctg gcctatgcca tcaccgccca cggtgcacag ggggcgagtg
     3781 aaaccttttc catcgcgctt gaaggcacgg aaggtaaccg gaaactgatg gccggctttg
     3841 agtcagccta cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc
     3901 gtcagggctg gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt
     3961 ttgagccgaa accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg
     4021 agctgcggga cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag
     4081 acagtcctgc acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc
     4141 cggcgtttga ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg
     4201 acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc
     4261 agttcgtggc cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg
     4321 atggcgtccg gattgcccgt gataatcctg acagtggtgt ggtggtaaga atcgccggtg
     4381 aaggccgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg gatatcccgg
     4441 acaacagcgt ccagccggga gccggaaatg gtgaaccggt cacggcagag gtactggcac
     4501 agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc
     4561 gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagcag gctgtcaggg
     4621 aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg
     4681 agagtgtact gcgtgaacca caacgggtgc gggaagcggt ccgggaagtt gcccgggaaa
     4741 atttgctgca ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag
     4801 agaaaacccc gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg
     4861 aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg
     4921 gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg
     4981 gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga
     5041 gcctttccgc tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg
     5101 caaccggcca ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg
     5161 gcaggctttc cttggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg
     5221 ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag cggccatact cctgatggcc
     5281 ctgtgggggc ctttgtccgg caccagttac ggtattgccg ggttgctgat gctggcagtc
     5341 agcaaccggc tgtaccgggc ggaagacaga gcggaacgtc tggcgctggt cgcctgcctg
     5401 ctggctgtta ttccggcgct taaccttgcc accagtgatg cagcggcggt ggccggtctt
     5461 gtgatgacgg tgctgaccgt tggtctggtg ttgtgtgccg gaaaatcatt accccgtttc
     5521 tggcccggag actttttccc gacgttctat gcctgtcatc tggctgtgct gggcgttctg
     5581 gcgctgtgac gggtgtggta tctttggccg caagaggatg attcgtcaga ggcagaacac
     5641 agcatgacag agcagaagcg accggtactg acactgaagc ggaagacaga gggaacagcg
     5701 cctgtccgca gccggaaaac catcatcaat gtcaccacgc caccaaagtg gaaggtgaaa
     5761 aagcagaagc tggccgagaa agcggcccgt gaagcagagc tggcggcaaa aaaagcgcag
     5821 gccagacagg cgctgtccat ttatctgaac ctgcccacgc tggatgaggc tgtgaatacc
     5881 ctgaagccct ggtggccggg attatttgac ggtgacacgc cccggcttct ggcctgcggt
     5941 atccgggacg tgttactgga agacgtggcg cagcggaata tcccgctctc gcataaaaaa
     6001 ctgcgcaggg cgctgaaggc catcacccgt tcagaaagct atctgtgtgc catgaaagcc
     6061 ggtgcctgcc ggtatgacac ggaagggtat gtgacggagc atatttctca ggaggaggaa
     6121 gcgtatgcgg gagcgcgtct ggcgaaaatt cgtcgccaga accggataaa ggcagaactt
     6181 caggccgtgc ttgatgagaa ataaaaaaag cctcccccag agagaggagg cagggaaata
     6241 aggcttaaaa ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc
     6301 acaataatac tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg
     6361 gaacggccag gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag
     6421 tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcctaggac
     6481 agactggcag cagtttgccc ggaaaagggc tgaacactgc caccggcgct gccggggaag
     6541 ggtgtaacga aaaaagcagc cttcattact ctaggagtca gaaggctgaa aggagtttat
     6601 gagatttgct tcttaatatc cggtcgggca gtatgaccgg acagttctga gtagcacaga
     6661 gtcttttccg cattaagtcg attttttata ttttgtgatt agtttcacaa aataaggtgt
     6721 tattcaatgt gtgctgcact attcaggggg gctgaaatac aggtggcatt tatctgatat
     6781 ggagaataac atgaggaaat atattccact ggcattattt atcttttcat ggccggtatt
     6841 gtctgcagat attcatggac gggttgttcg tgtactggac ggtgatacca tcgaggtcat
     6901 ggactcactg aaggcggtgc ggatcaggct ggtaaatatt gatgcaccgg aaaagaaaca
     6961 ggattatggc cggtggtcaa cagacatgat gaaatcactg gtcgcaggaa aaacggttac
     7021 cgtcaca
//
LOCUS       DBBBSB010000174         6912 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.177, whole genome shotgun sequence.
ACCESSION   DBBBSB010000174 DBBBSB010000000
VERSION     DBBBSB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.177"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..694
                     /locus_tag="ACIUS0_003829"
     CDS             <1..694
                     /locus_tag="ACIUS0_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGB0221382.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACIUS0_003830"
     CDS             complement(1033..1803)
                     /locus_tag="ACIUS0_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221383.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACIUS0_003831"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACIUS0_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACIUS0_003832"
     CDS             complement(2294..2515)
                     /locus_tag="ACIUS0_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221384.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACIUS0_003833"
     CDS             complement(2516..3199)
                     /locus_tag="ACIUS0_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGB0221385.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACIUS0_003834"
     CDS             complement(3263..3574)
                     /locus_tag="ACIUS0_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221386.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACIUS0_003835"
     CDS             complement(3571..4473)
                     /locus_tag="ACIUS0_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGB0221387.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="ACIUS0_003836"
     CDS             4913..5872
                     /locus_tag="ACIUS0_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGB0221388.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="ACIUS0_003837"
     CDS             5872..6267
                     /locus_tag="ACIUS0_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGB0221389.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="ACIUS0_003838"
     CDS             6450..6830
                     /locus_tag="ACIUS0_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGB0221390.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1889 a         1787 c         1675 g         1561 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac ca
//
LOCUS       DBBBSB010000175         6848 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.178, whole genome shotgun sequence.
ACCESSION   DBBBSB010000175 DBBBSB010000000
VERSION     DBBBSB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.178"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..236)
                     /locus_tag="ACIUS0_003839"
     CDS             complement(<1..236)
                     /locus_tag="ACIUS0_003839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221391.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACIUS0_003840"
                     /pseudo
     CDS             complement(325..1203)
                     /gene="narH"
                     /locus_tag="ACIUS0_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACIUS0_003841"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="ACIUS0_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACIUS0_003842"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="ACIUS0_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGB0221392.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(6734..>6848)
                     /locus_tag="ACIUS0_003843"
     CDS             complement(6734..>6848)
                     /locus_tag="ACIUS0_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221393.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
BASE COUNT         1652 a         1773 c         1783 g         1640 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgaaccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgt
     2761 atatactcgt ggcctttgcc ggaggagtca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcatt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcagtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 aatctaaagg gttacatatt aactatattc tcaacaagac cagacactta tagaattagt
     6721 gatgatttat tcactaaaaa ggccatgaat tggaggaaaa cgatctgtcc agaaagtcta
     6781 tcggttcctc tcttattgcc tgattgtaat aatcgttatt atagtaagta ttgtttattt
     6841 catctaag
//
LOCUS       DBBBSB010000176         6605 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.179, whole genome shotgun sequence.
ACCESSION   DBBBSB010000176 DBBBSB010000000
VERSION     DBBBSB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.179"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1077
                     /locus_tag="ACIUS0_003844"
     CDS             <1..1077
                     /locus_tag="ACIUS0_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HGB0221394.1"
                     /translation="AAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLHDAYKASDERLYVNKQNKNSRS"
     gene            1120..1623
                     /gene="yeaK"
                     /locus_tag="ACIUS0_003845"
     CDS             1120..1623
                     /gene="yeaK"
                     /locus_tag="ACIUS0_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGB0221395.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACIUS0_003846"
     CDS             complement(1624..1728)
                     /gene="yoaI"
                     /locus_tag="ACIUS0_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGB0221396.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1898..2344
                     /locus_tag="ACIUS0_003847"
     CDS             1898..2344
                     /locus_tag="ACIUS0_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGB0221397.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACIUS0_003848"
                     /pseudo
     CDS             complement(2301..3119)
                     /gene="nimR"
                     /locus_tag="ACIUS0_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3216..4396
                     /gene="nimT"
                     /locus_tag="ACIUS0_003849"
                     /pseudo
     CDS             3216..4396
                     /gene="nimT"
                     /locus_tag="ACIUS0_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="ACIUS0_003850"
     CDS             4451..4798
                     /locus_tag="ACIUS0_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGB0221398.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACIUS0_003851"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="ACIUS0_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGB0221399.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="ACIUS0_003852"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="ACIUS0_003852"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGB0221400.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /locus_tag="ACIUS0_003853"
     CDS             6315..6413
                     /locus_tag="ACIUS0_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGB0221401.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACIUS0_003854"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="ACIUS0_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGB0221402.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1601 a         1601 c         1618 g         1785 t
ORIGIN      
        1 gctgctgagc agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag
       61 tctgccaata atacccatgg cttaaacctg actgcccata aatacccggg cttacgcggc
      121 acactacaaa ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc
      181 gcttttgatc aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg
      241 atggccgaag agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat
      301 gagccggtta atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt
      361 attggcatcg atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc
      421 atttaccagg atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc
      481 gcagggcagc taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc
      541 ttttacactc atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc
      601 gattcggggc gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt
      661 tacgtccatg acttacctgg cggcattcgt gtctcgttat ccattgatat tctttacttt
      721 atcacgtctt cgaggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat
      781 atggtccgga tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg
      841 atgactggac tgtataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa
      901 ctggtacaat ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa
      961 ataaatgaca ccctcggtca tcaggagggg gatttagcga ttacgttaca tgatgcgtat
     1021 aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg ttcatgataa
     1081 ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat ggctaaagga
     1141 agcgtgacgc atcagcagtt aatcgcttta ttatcacaag aaggtgctaa cttccgcgtc
     1201 gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg taccgctcta
     1261 ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa tcaacatgtt
     1321 ctggcgatcc tcgcagccga tcaacaggcc gatctgagcc aactcgccag ccatataggc
     1381 ggattacgcg cttcgttggc cagcccggcg gaagttgatg aactcacagg ctgtgtcttc
     1441 ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga ccctctactc
     1501 ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc cgttattttg
     1561 aaaaccgcgg attatttgcg cattgcgcaa ccagaactgg tcaatttccg ccgcactgcg
     1621 tagctagcct atccgttcaa taagcagaac ggacaaaacc agcacaaccg caatagcaaa
     1681 aaacgatgat gtgataatca gtgtctcgac gaacatttga tcgttcatag catgcccccg
     1741 ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga tgacagttcg
     1801 cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag caagcataaa
     1861 gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca ctttgctgat
     1921 cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg ccgtttcaat
     1981 tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt ggattgaaaa
     2041 acaggggcta agtatcggga taatcattct gacgattggc gttatggccc ccatcgccag
     2101 cgggacgcta ccgccctcaa ccttgattca ctcgtttctg aactggaaat cgctggtggc
     2161 aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacgt taatgggcag
     2221 ccagccgcaa ctggttgctg ggctgctggt gggcactgtt ttaggcgtag cgctgtttcg
     2281 cggcgtaccg gttggcccgc ttattgccgc gggtctggtt tcgctgattg tggggaaaca
     2341 gtagttaatc ccgcaatata tcggcctggc gtttgcccca agcctttctt aaacatagta
     2401 ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac agtatcgcct
     2461 ttcactaatc cctgaagcgc cataatcagc tgtaactgct gacgccactg gcgaaaactc
     2521 aaccccgtct ctttcactat caggcgggca agattgcgtt cactcatcgc aaaaaagcca
     2581 gcccattgcc ccaatgcccc ccactcgacg ggccctttcg ccatcatctc caccatcgtg
     2641 cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg ctgagggagt
     2701 tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg agtcgtcgtc
     2761 ctgtttgcca gggttaaaat caattcccgg cataagggcg atattttcaa tgtgcaacat
     2821 ggtcggcatt gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt
     2881 cacttgattg ctatgcttca ccccgcccgg tatccaaacc gcatattgtg gcggcaccat
     2941 ccacaaagca ttttccaccg tacaggtaat tgcaccatgt aatgccagta tcagctgtcc
     3001 tttgcggtgc tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc
     3061 gaccgcggct tcatggtgtc ggtgggttca tagccattga gattcaacct gtgcatcatt
     3121 ttgtccgaac ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg
     3181 taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg
     3241 gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca ttacgcgtca
     3301 cctttaccgg cgcagcgccg ttactggata cgattcgttc cgcttactcg ctgacgacag
     3361 cgcaaaccgg cttattaacc accctgccat tattggcctt tgcgctaatc tcacctctgg
     3421 ctgccccggt ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga
     3481 tctgtgcagg tatcgcaatt cgttctctcc cttcgcctta cttattattt ggcggtacag
     3541 cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta attaaacgcg
     3601 atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag
     3661 cggcactggg atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg
     3721 cgttgttcat gctgatgtgt tttcctctgc tggctctttt tttatggctg ccacagtggc
     3781 gaagtcagca acatgcaaat ttgagtacct cacgcgcctt acatactcgg ggtatctggc
     3841 gttcgccact tgcctggcag gtaacattgt ttcttgggat caactcactg gtctattacg
     3901 tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg
     3961 gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg atcgcacttt
     4021 tcttacatca tgtgaaagac cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag
     4081 tgggcgcggt tgggctctgc tttatgccag cgcacgcgat cacctggact ctgcttttcg
     4141 gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt cggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtcttgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggct gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctga
//
LOCUS       DBBBSB010000177         6549 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.180, whole genome shotgun sequence.
ACCESSION   DBBBSB010000177 DBBBSB010000000
VERSION     DBBBSB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.180"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            158..505
                     /locus_tag="ACIUS0_003855"
                     /pseudo
     CDS             158..505
                     /locus_tag="ACIUS0_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            558..953
                     /locus_tag="ACIUS0_003856"
     CDS             558..953
                     /locus_tag="ACIUS0_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGB0221403.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            994..1737
                     /gene="cmoA"
                     /locus_tag="ACIUS0_003857"
     CDS             994..1737
                     /gene="cmoA"
                     /locus_tag="ACIUS0_003857"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGB0221404.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1734..2705
                     /gene="cmoB"
                     /locus_tag="ACIUS0_003858"
     CDS             1734..2705
                     /gene="cmoB"
                     /locus_tag="ACIUS0_003858"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGB0221405.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="ACIUS0_003859"
     CDS             complement(2870..5299)
                     /gene="torZ"
                     /locus_tag="ACIUS0_003859"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGB0221406.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5324..6424)
                     /locus_tag="ACIUS0_003860"
     CDS             complement(5324..6424)
                     /locus_tag="ACIUS0_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGB0221407.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1501 a         1715 c         1553 g         1780 t
ORIGIN      
        1 ataatgggct tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac
       61 tcatagctaa ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt
      121 aggaaccatc tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt
      181 tcatgcatca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
      241 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
      301 tatgacgcaa aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
      361 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
      421 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
      481 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca
      541 ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa
      601 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
      661 gtggttttag cgaactgcaa agcgctattt gcattcatgg taacgcggtg gaatatattc
      721 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
      781 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
      841 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
      901 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
      961 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     1021 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg
     1081 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     1141 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     1201 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     1261 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     1321 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     1381 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa
     1441 atttatcaag gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     1501 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     1561 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     1621 tccgtggaaa cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg
     1681 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     1741 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     1801 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt
     1861 ccaacacggt ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg
     1921 taaccgccga aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta
     1981 tgcggaacct gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     2041 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     2101 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     2161 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     2221 cggtgcgtaa actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac
     2281 agcttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     2341 gttcgccgct ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg
     2401 tgctggaaac gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt
     2461 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     2521 agaagtgtgg ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc
     2581 agcgacgcac tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc
     2641 cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc
     2701 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc
     2761 ttgctaattc aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag
     2821 ttttacgttt gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa
     2881 cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca
     2941 ccagcgcaga gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta
     3001 acacgttcgc actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat
     3061 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     3121 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt
     3181 cggtgtgaat agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat
     3241 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct
     3301 cgtcggcggt acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct
     3361 tatagccaaa tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca
     3421 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     3481 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     3541 tgacgcgttg cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca
     3601 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     3661 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     3721 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     3781 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     3841 cgacgatcat ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg
     3901 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     3961 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     4021 tcgccgtcat tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca
     4081 tttccggcag cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga
     4141 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     4201 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     4261 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     4321 tttccgccgt ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     4381 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     4441 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     4501 tccaggtggc attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa
     4561 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
     4621 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
     4681 tgttttccag aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca
     4741 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
     4801 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
     4861 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct
     4921 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
     4981 cgccacgacc tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat
     5041 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta
     5101 tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca
     5161 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
     5221 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
     5281 cacgtcttgt taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
     5341 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc
     5401 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
     5461 cggcaataga ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
     5521 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
     5581 gccattgatt gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac
     5641 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
     5701 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
     5761 cgacggtgag atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga
     5821 aaggataaat atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg
     5881 ccgcgttgtc atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga
     5941 tacaggtttc gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg
     6001 aggcaatatc catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt
     6061 ttaattcttt ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac
     6121 tgtctatttt gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa
     6181 ataaataatc catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt
     6241 tctggttcga aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc
     6301 aggaaaggca aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc
     6361 cgccgccacc aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc
     6421 gcatagattc ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa
     6481 cgaattaacc gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg
     6541 atttattac
//
LOCUS       DBBBSB010000178         6532 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.181, whole genome shotgun sequence.
ACCESSION   DBBBSB010000178 DBBBSB010000000
VERSION     DBBBSB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.181"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..353)
                     /locus_tag="ACIUS0_003861"
     CDS             complement(<1..353)
                     /locus_tag="ACIUS0_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGB0221408.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..934)
                     /locus_tag="ACIUS0_003862"
     CDS             complement(350..934)
                     /locus_tag="ACIUS0_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221409.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(921..1298)
                     /locus_tag="ACIUS0_003863"
     CDS             complement(921..1298)
                     /locus_tag="ACIUS0_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGB0221410.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACIUS0_003864"
     CDS             complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACIUS0_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGB0221411.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1748..2776)
                     /locus_tag="ACIUS0_003865"
     CDS             complement(1748..2776)
                     /locus_tag="ACIUS0_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGB0221412.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2834..3181)
                     /locus_tag="ACIUS0_003866"
     CDS             complement(2834..3181)
                     /locus_tag="ACIUS0_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGB0221413.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(3218..4723)
                     /locus_tag="ACIUS0_003867"
     CDS             complement(3218..4723)
                     /locus_tag="ACIUS0_003867"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGB0221414.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQSEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(4713..6306)
                     /locus_tag="ACIUS0_003868"
                     /pseudo
     CDS             complement(4713..6306)
                     /locus_tag="ACIUS0_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6303..6506)
                     /locus_tag="ACIUS0_003869"
     CDS             complement(6303..6506)
                     /locus_tag="ACIUS0_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGB0221415.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
BASE COUNT         1420 a         2069 c         1574 g         1469 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacatc gggattttca
      481 ccacatcaat ggggtaacga tttttgccgt caatacgccg catcacatgc cagcgaccat
      541 tcgccagttg ctgaataaac gcatcccgga aaaggtattt ccccactttc agtacgctgc
      601 cacgatgacg tgagttgctg ccatgccggg agcgggccag tctgacctgt gcggtaccca
      661 gcttaatggc gggcaggtta ccccggttaa cacgcatgcg ggcatacatt tttcctgagg
      721 ggctggcttt aagcagcctg acacgctccc tgaccgtttt caggggaata cctttcacct
      781 ggttatcccc ggcaacggta ttctgcgcaa cctgccgtgt ggcgacagaa acaatctttg
      841 ccgccacacg gtttaccgcc catgcgctgg tctgtggcac catgcgggta tcgaggctgt
      901 tcagattgcg gatggcattc tcaagtccct tcatcatatc gcctcattct ggcattattc
      961 tgtggggcag gactgccccg ccttaaccgg atataacagc atcccccgtc atccggagta
     1021 atacgatcca caaagaaaga atcatccccg atagttaacg tatcctgacg gcgtaactgc
     1081 gatatatctg ccgttttcac aaacaggcac ggtgaggaat cctcaagccg gatccccccg
     1141 gcaacaaaag aaatactttc gggatcatca aaaacccctg tcaggtgtgt cccctcaagt
     1201 tctccggacg taatgacggc gctgatcccc atatcacgga gaatgacttc attgacaccg
     1261 gcaagcgcag catcaaacag attatcgaaa ccgtccacga catctcctgt tactgccttt
     1321 ttgccagacc acctgccgcc atgctggcag ccacctcagc agaaacacga aacgacgttc
     1381 ccggtgaaac aaatatcact gaaccattac attttgtatg cagtgtcgcc agtgccacca
     1441 ccgtaacctg ccccgtttcg tctccggtaa caatgcctgg ctgttcagga actgactcgc
     1501 ctttatgcgc ggtcggatca tctttcgatg gtgagtacgt atccggctct gcgacctccc
     1561 ccgcatctcc aagctcttcc tccagttccg ccactcgctg cgcaatatct gccgcactac
     1621 cggagacatc cggttcacgc cccagcctct cagccagctc acaaagacgt ttcaggtttt
     1681 cttctttcgt agccataccc gctcctgatt gaatgaaaaa cgggagcatt tagctcccgc
     1741 tcacggatta tttcacctgc accaccacaa actcatccgg atccggcagc accatcagcg
     1801 gcgcggactg cgtcatggtg aattcacgcg ccggatcacc cacggtcagc cagtgtttcg
     1861 gataacggga agacgccacc acgccttcag acaatgcctg cgcatcctga atggcaccgt
     1921 agcaacgaat accctctgcg gccgtatttc ccagtaccag tgtgccgtcc ggcagataac
     1981 gtttttcggt accgtcctct gccacatagg acgtttttgc caccacgatt gccagatcac
     2041 cataataccc tttgaaggac accaccgccc ccaggtcttt cactgccgtt tccagctgtg
     2101 aatttgagcc acgacgggta tccagttttt cgcggaacag cttaaagcca ttcagcagac
     2161 gccagacctt accatccata atggcaatat tgatggtgcc ggaagcaaaa tcacagtacg
     2221 catccagatc atgcgtcgga tcaaaggtat cgcggtcctg agcgctccac tccgtccctc
     2281 ctgcctgcgt aatgttattt cccaccgacc ggccaaaatc gacctcaaca gtttcaaact
     2341 gctctccttc catggtgtat ttgccataca gcacggcatt caccgcctgc atctcttcca
     2401 cctggacaat ggcgtgctct tcctgtttga ggttatcggt gatgatacgc aggcgacggt
     2461 aagccgggcc gttcagctgg gccggatctt caccgggaag acgctccacc gcctgctggt
     2521 aattaaattc gtgtttcggc ttaacgtagc ccgggcgcag tacacgagtt tcaccgccac
     2581 ggtgacgcaa cacctttcct tcaacaaccg gggagacata ggccgccacc ggcgtttttc
     2641 cggtaatttt gtccagcatt acctcttcgg tatggaaatt caccgtacgg cggaaaaaca
     2701 gctccagaaa cagcgcacga aatttaactt tttgttcggt ataaccgagt aactggcggg
     2761 tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc taccgcagtg
     2821 acctgataaa atgttacggc agcgctgcgt gactcagggc actgccggca aaggcattcg
     2881 cctttttgtg tgtatccact ctctcaggcc agtggatggc ctccgtcgca aaggtccccg
     2941 acttgtaata cgtcagcacc gtctctgtac cttcaagcgg cagtaccagt atgccaacca
     3001 catggcctgc cttctgtccg tcccagacca ccagtttccc ggtatcctca tccagcatca
     3061 ggggcgtcag agccggtgtt gccgaggaaa tcccgctgca gcctgtggcg gtatgggccg
     3121 gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatggtt ttcgttacca
     3181 tattgtaaaa acctcatatt gatggtcagc actgacttca tggcatggcc atgagcattt
     3241 tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc gctgccggtg
     3301 aatggttcgc catgaaatgt tcaaacatgg cggttgtgga tgcagaaacc ggttctgccc
     3361 tgcctgaggc tgccgccagt acagcccggg cattctccac ggtcatcccc ggacaggccg
     3421 ccagtttttc agactgcgcc tctgcccctt ttgcctcatc cagggccata atctgatcac
     3481 gaagtgaggg tccggcattc gcctgcggtg aagcagccag aatcggacgg gctttttcca
     3541 ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgtcct ttcgcctctt
     3601 cacatgccat aatgcgatcg gcttcactct gcgcagatac accagattgt tgcggtgccg
     3661 ccacggccag aatcgcccgg gcctgctcaa cgctcatacc ctgttgccct gccagcatcg
     3721 tggccagttg ttcacgtcct ttcgcttcct gacacgtcag gatccccatc actcgctggt
     3781 tctcctgcgc ggcagcctcc gttgcagtta attgcggcat agtgcctcct gtatcgtgtg
     3841 tgttcagcgt cgcagccatc acgctgatgg cgtccgacgc attgattaat tcatccgcca
     3901 gttcggcctc aataccggac tgaccttcaa aaacggcggc ctctgttccc ataaccgcat
     3961 ccacagataa accggtatac atcgccactt tttcagcaaa catccggcgc gccgcatcaa
     4021 tgcgctgctg catgtcctgg cgaacctttg ctggcaggga ttcaaactga ttgccatcca
     4081 ccttgtgcga tccggcgtaa atcagcgtga tatccacacc ggcctgtgcc agatgaccgg
     4141 catagctgac atggcccatc atcaccccaa tggagccgat acgggatgtc tgggtaacca
     4201 gccgtcggga gcaggccgat gccagtaaca tggcggctga acaggccgtg tcattgcaca
     4261 gtgcccagac aggcttcagc tgccggagac ggtaaatcat gtcagcgcag tcaaacgcgc
     4321 cggtggcctg cccgccagaa ctgtcaatgt ccagcagtac gccccgtacc tgagtgtccg
     4381 ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc agtcattccg gaaaatggcc
     4441 gcatgccacc cagccggtgt accagcgtgc cggtcaccgg cagtacagca ataccgttca
     4501 ccacccggta aacacgggca ggtcgtttgc ctccggccat gtactcgtcc gtttcagcca
     4561 gcatcccggg agcgtcaagc tgtacctgct gttgcggtac cgaaaggctt gctgcgccca
     4621 tctcacgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggct
     4681 cattgaatgc cgcagcgata atatgtgaaa gattacgtct cacgtgatgt tgtctcctct
     4741 tccggcctgc gactctccgc tatctgctgc tgatacgcct gggctatcca caccggacgt
     4801 gagagtccgg ctttttcgcg ctctgcggat tccctgacct gctggcggaa aatgtcctga
     4861 taatcttcgc ccatcagtgc cagttctttc tcatacgtgc tcagtccggc ctcaatgcgc
     4921 atcaccgatt cctggacctc cttgagcccg tcaatggcca ttcttccgga gctaatccac
     4981 tctgcccgtg accaggctga acgcgcctgg taaaaatcaa aacgcgcccg tggcggacga
     5041 ataatccccc gaagaagcgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg
     5101 gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc ggaggcgcgg
     5161 gcacttgagt aactgacctt cgagtaatca cgggacaact gttcgtagga aacgccaaga
     5221 ccggctgcaa tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc
     5281 tgcgctgtct gcaggttcag cgcatcgcca ggaaaaaagg tgcggaattt taacaccgcc
     5341 cagcgtcacg ttattcgtgt cataccagcg ggtgaacttc tccagaatat taataagcgg
     5401 attatcctgc tgaccttgcg gtgcccccgc gatatattca aatgcctttt cggtatcaag
     5461 ttcactttcg attgtggctg catacatcgc cttcacaatg gccgactgaa gctgcgtggc
     5521 ctgcagggag tccagcatct tcatccgctc cattacgctg taaaactgat tagccccacg
     5581 ggtctgcccg tcctccaccg gctcaaaaat atgcagcatg gccggacgcc cggtgggaag
     5641 ctcacgcggg atccgctccc agcgtccggc accagagaac ggaaaatcat cctcacagat
     5701 atggtacgca atcgcccgcc cataccgatc gacctcaacc ccggcccgca gaaagtggtt
     5761 ccccataccg tgtcctggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     5821 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaaggc
     5881 atgcacgccc accccttcac ggataaattc cgtaaacgtg cgtttccctt ccacgtcgat
     5941 ctcgccaaac attccttctg cgtattccga ccaggctgct tccacctccc cgacaaaact
     6001 ttttgccgtg gtctcccgca tccccagcca acgccagttc agacggtagc tgataagaaa
     6061 catgtgcccg acgatatgat ccttatgcag ggccaccgca ttagccgcga taccgttatt
     6121 gcgcaccaga tcatctgccc gggcattccc cagacgcaat gcaggcaaca gggccgcatc
     6181 ggcactctgc gccggtggta accactctgc catttgcccg ccaaatcctg cgccgcctcc
     6241 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     6301 tttcataacc tcactcccag cggtcggcgg cgtcgggtcg tacccagaac cgactccgca
     6361 tcgttgatcg cacggttaag ctcattcagg gacgctgccg tatattcgat cctgcgaccg
     6421 tctttctgta ccgacacaac ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc
     6481 gcctgcagtt cagcgactgt aaccattcac tcctccggac agcttcgctg cc
//
LOCUS       DBBBSB010000179         6466 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.182, whole genome shotgun sequence.
ACCESSION   DBBBSB010000179 DBBBSB010000000
VERSION     DBBBSB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.182"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            38..778
                     /locus_tag="ACIUS0_003870"
     CDS             38..778
                     /locus_tag="ACIUS0_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGB0221416.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACIUS0_003871"
     CDS             837..1223
                     /locus_tag="ACIUS0_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGB0221417.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACIUS0_003872"
     CDS             1232..1561
                     /locus_tag="ACIUS0_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGB0221418.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACIUS0_003873"
     CDS             1533..4574
                     /locus_tag="ACIUS0_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGB0221419.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACIUS0_003874"
     CDS             4574..4903
                     /locus_tag="ACIUS0_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221420.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACIUS0_003875"
     CDS             4903..5601
                     /locus_tag="ACIUS0_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGB0221421.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACIUS0_003876"
     CDS             5606..6349
                     /locus_tag="ACIUS0_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGB0221422.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6286..>6466
                     /locus_tag="ACIUS0_003877"
     CDS             6286..>6466
                     /locus_tag="ACIUS0_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGB0221423.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETE"
BASE COUNT         1531 a         1504 c         2111 g         1320 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaat
//
LOCUS       DBBBSB010000180         6451 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.183, whole genome shotgun sequence.
ACCESSION   DBBBSB010000180 DBBBSB010000000
VERSION     DBBBSB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.183"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(16..870)
                     /locus_tag="ACIUS0_003878"
                     /pseudo
     CDS             complement(16..870)
                     /locus_tag="ACIUS0_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            978..1256
                     /locus_tag="ACIUS0_003879"
     CDS             978..1256
                     /locus_tag="ACIUS0_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221424.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1257..1784)
                     /gene="gadE"
                     /locus_tag="ACIUS0_003880"
     CDS             complement(1257..1784)
                     /gene="gadE"
                     /locus_tag="ACIUS0_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGB0221425.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2583..3155)
                     /gene="hdeD"
                     /locus_tag="ACIUS0_003881"
     CDS             complement(2583..3155)
                     /gene="hdeD"
                     /locus_tag="ACIUS0_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGB0221426.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3410..3742
                     /gene="hdeA"
                     /locus_tag="ACIUS0_003882"
     CDS             3410..3742
                     /gene="hdeA"
                     /locus_tag="ACIUS0_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGB0221427.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3858..4184
                     /gene="hdeB"
                     /locus_tag="ACIUS0_003883"
     CDS             3858..4184
                     /gene="hdeB"
                     /locus_tag="ACIUS0_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGB0221428.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4248..4894
                     /locus_tag="ACIUS0_003884"
                     /pseudo
     CDS             4248..4894
                     /locus_tag="ACIUS0_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4936..5466)
                     /locus_tag="ACIUS0_003885"
     CDS             complement(4936..5466)
                     /locus_tag="ACIUS0_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGB0221429.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5622..6188)
                     /gene="slp"
                     /locus_tag="ACIUS0_003886"
     CDS             complement(5622..6188)
                     /gene="slp"
                     /locus_tag="ACIUS0_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGB0221430.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1880 a         1338 c         1380 g         1853 t
ORIGIN      
        1 ttacctagtc ataaatacat tctggcggct accttcatcc actaatgccg tgacatacat
       61 gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt
      121 ggtttcatca accgtcgggt cggagaattt cagcgtgccg gtctggctgt aacgtttacc
      181 attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc
      241 gacctcttct ttcatgcgta agaaatcttg caccgactgc gtgagatcga cataaatcgg
      301 gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac
      361 ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag
      421 attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt
      481 cagctgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct tcagcaacga
      541 tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga
      601 gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat acagcgaatc
      661 gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg
      721 gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt
      781 gaccgaacca ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc
      841 cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag
      901 ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat
      961 tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca
     1021 gtaaggtggg atacaggcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg
     1081 ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact ggtggataca
     1141 cataccaggg gaatcaagtc gttttagcct ctcctttagt aatcaccggg ggtaatgaaa
     1201 tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg tcctgactaa
     1261 aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt cagcttgtac
     1321 ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata
     1381 ttgctttcat ttttattttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa
     1441 cataattgtg aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg
     1501 atactttctt tgcggctaac aaaataaata ggcgctttag cttttagttt actgatgtga
     1561 taaggtgcca aaacaataat tctctcggca tctaatttct ccagaaattt aatcgcttct
     1621 tcatcaagga tatgattgtg ataggtatca atgataacct tgaagggttt attgcctact
     1681 tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa
     1741 aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc
     1801 gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc
     1861 aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta
     1921 atcaatactt cagatttatc catcacgcac tccatatatt gatcctaagt aagattcctg
     1981 gttgttatca gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta ctgcccaaga
     2041 ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat
     2101 ccgatttaaa tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt
     2161 cttttaacgc attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat
     2221 agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac
     2281 ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc
     2341 ccacagcact tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg
     2401 aaatggctaa tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaataat
     2461 cactagcagg taattatttc aatgatagtg cgcaattgat ctacaacact gcgtagcgga
     2521 gagagtatta atcggatcat agtcacatca agtgactatg atccgggtga caaccggggt
     2581 aattattgct gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc
     2641 agttcaattc ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg
     2701 aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc
     2761 attgaacgct gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg
     2821 gcaataaatg ccgcaatggc aaaaatgccc agctccggtg cacggatgaa gaaatagccg
     2881 atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg
     2941 cggttgctga ataacccgac aataagcgca atacccgagc agattaataa tgcacccact
     3001 actgtgctta aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata
     3061 aacagcagca cggcaataaa ctggattgct ctgcgatgct ttttcagcat ctccagatca
     3121 aacttcaaaa ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag
     3181 aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa
     3241 aaatttccta attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc
     3301 tgattttgat attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt
     3361 cgaaattgat tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt
     3421 attaggcgtt attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc
     3481 gcaaaaagca gctgataaca aaaaaccggt caactcatgg acctgtgaag atttcctggc
     3541 tgtggacgaa tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga
     3601 taaaccagaa gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt
     3661 tcaggcttgt actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga
     3721 caaaattaag aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg
     3781 aacaggccgg aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat
     3841 attgaatggg ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg
     3901 ctgtagcggc tttgtcactg gtgaacgcac aatctgcgtt ggcagccaat gaatctgcta
     3961 aagatatgac ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat
     4021 ggtggatgct gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa
     4081 ccgatctcac tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt
     4141 tgtatacctt caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa
     4201 aggagtagca agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat
     4261 ttattatacg tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa
     4321 tgcgcggcaa aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt
     4381 ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg
     4441 accccagccg tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca
     4501 ttctcgttcg taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg
     4561 ccgcataggt atggttattg gcagcggtat gtacgaactg ggtatttatg gttcagtgat
     4621 gaccttgttg gtgctggaag tcttccatca attaaccttc cgcctgatga acaaaaatta
     4681 ccatttacag ctgacattag tgaacggtaa tacggtatca atgctcgact ggtttaagca
     4741 gcaaaaaata aaaactgatt tagtttcgtt gcaggaaaat gaagatcatg aagtggtcgc
     4801 cattgatatc cagcttcatg ccacaacgtt gatagaagac ctgttgcgat tattaaaagg
     4861 tatggcaggg gtgaaaggcg tttcaattag ctaatctgtg tggcagcgta gccagactca
     4921 ccgtaagcct gaaattcaca ccagataatc aatatgctga tggcgtacca gctcattgat
     4981 ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg cagtagaacg tttttagcga
     5041 tattttcagt ttctccagaa tatcttcagt tgtcatttga ttcgcaatgc agctcagaac
     5101 ctgatgttgc ttgcgcgaaa gtgacacatt cgcttcaggc acagagcagt aactgccatt
     5161 gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga acaaaggtca ccggtacttt
     5221 ccccagacag cgtttaatat taaaggggga gaaaacaatg acatggacag gttttaatcg
     5281 ttcaatttga gtgagaaaat tagaatgaaa tacattattc attaatgtat caataatgac
     5341 gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt
     5401 acctttactc agaatgtttt tcatcgcggt gaagaacatc gtatccctgg taattataag
     5461 aaacataacc gactcctggt gcggacatta actttataat cagtttgcca aaggtaatta
     5521 acaggacagg gcttataata ataatagtta gcttactgta tattaatttt atgtaataca
     5581 tgatgcagat gctgagaaag catctgcatc tttcgatggt gttatttgac cagctcaggt
     5641 gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc cggccagaat
     5701 gcaccatagc cgtaatccca caggttatag gtggtattaa ccacttctct caaatgccac
     5761 acctggatgc cctgcatatt cacttccagg aagttatacg ggactttatt gataaagcct
     5821 ggttgttcac cctgaatggt accgaggatg gtaacaaagt gattacgata gttcactgga
     5881 tcaaggaagc cgctttgtct ggcgagcagt cggccctgat agttggcttc aatatcaggc
     5941 ttcgcatagc tatccaacgg taatacagcg atttctaaca acgtatccgt tttgccattg
     6001 ataacgttga taaccttccc accaaagcgc gcttgttgac caacatataa ccccggctgg
     6061 ttatgaacag caacaaaact tttttgaata tcaggttggt tattgccttt gatattttgc
     6121 ggaattgaac tacatgcggc aagcaaaaat gaaaggctga ggatgagtgc accttttgtc
     6181 atgttcatgt tactatcctt atcaacaaat attaaaacca taatagatat ttctttatag
     6241 ttttcatctg attctgaggt gatatgcctg atagtaacgt aaacctgtct gatgtttata
     6301 tcccttctgt ttagttcaat tccgttattt tggggcgtgg tatgagtatc catcgcccaa
     6361 taatataacc ctttgtttta aaattacata aaatctattt ttattcaatg attataaagt
     6421 gctaataatc aatattatgg tattatattc t
//
LOCUS       DBBBSB010000181         6374 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.184, whole genome shotgun sequence.
ACCESSION   DBBBSB010000181 DBBBSB010000000
VERSION     DBBBSB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.184"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..378
                     /locus_tag="ACIUS0_003887"
     CDS             <1..378
                     /locus_tag="ACIUS0_003887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221431.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACIUS0_003888"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACIUS0_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGB0221432.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACIUS0_003889"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACIUS0_003889"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGB0221433.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACIUS0_003890"
     CDS             complement(1787..2476)
                     /locus_tag="ACIUS0_003890"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGB0221434.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACIUS0_003891"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACIUS0_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGB0221435.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACIUS0_003892"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACIUS0_003892"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGB0221436.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACIUS0_003893"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACIUS0_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGB0221437.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1620 a         1716 c         1578 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DBBBSB010000182         6328 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.185, whole genome shotgun sequence.
ACCESSION   DBBBSB010000182 DBBBSB010000000
VERSION     DBBBSB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.185"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..289)
                     /locus_tag="ACIUS0_003894"
     CDS             complement(<1..289)
                     /locus_tag="ACIUS0_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221438.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWGRQHERDTGGGDGGLTTAERQRLKEPERENRELRRSNDILRQASAY"
     gene            complement(462..780)
                     /locus_tag="ACIUS0_003895"
                     /pseudo
     CDS             complement(462..780)
                     /locus_tag="ACIUS0_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(924..4232)
                     /gene="icsA"
                     /locus_tag="ACIUS0_003896"
                     /gene_synonym="virG"
     CDS             complement(924..4232)
                     /gene="icsA"
                     /locus_tag="ACIUS0_003896"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HGB0221439.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4761..5963
                     /gene="virA"
                     /locus_tag="ACIUS0_003897"
     CDS             4761..5963
                     /gene="virA"
                     /locus_tag="ACIUS0_003897"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HGB0221440.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            6142..>6328
                     /locus_tag="ACIUS0_003898"
     CDS             6142..>6328
                     /locus_tag="ACIUS0_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221441.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1912 a         1453 c         1107 g         1856 t
ORIGIN      
        1 aataagcgga agcctggcga aggatatcgt tactgcggcg cagttcacga ttttcacgtt
       61 ccggctcttt cagacgctga cgttcagcgg tggtgagccc tccatcaccg cccccggtat
      121 cccgctcatg ctggcgaccc cagacacgca gagtctccgg cgtacagcca atctttggag
      181 caatggaaca aattgtcgcc cattgtgagt catattcgcc ctgactttcc agaaccatac
      241 ggactgcccg ttgacggact tcgggggaaa aacgagtatt tttagtcatc ctgtttacct
      301 ctttctcagg aagtttagtc tccaggattc ccggggcggt tcagttcagg ctgtacgctt
      361 ccccatgtga tttgcctcgc cacttcaccg ggctcaggac cccgtaggat cttcaccgaa
      421 aaaaatgcat caccagttgc tttattattt gttttaggta gacctgttac taatttatac
      481 ctactctgac caagtctgga taagaaaggc acacctgcac atttctactt ggaaatgttt
      541 tcttttacca ttttgctcaa aaatttcaac atccaatatt cctcgtacag aactactcaa
      601 gtcctgaata gaaaaagttc cagctggtac agtagagtca tatattactc gaccttgttg
      661 agatacaaca acacgtgcat tagtttctgc aataccagta atctgaggcg cataaccacg
      721 aatatttggc ggtagcattc gttcatcgct ttctaatgca attcctgtgt aacgccaagc
      781 aacagcgcat tctggcgcat gatctggtaa acacgtttgg cattgatcgc aggcatacca
      841 tcatgtgcac aaaaacccac cgagctttta cacggtgggt cccagagaaa tgcaggacat
      901 caacacgccc tgcattttta ttatcagaag gtatatttca cacccaaaat accttgggtg
      961 tctctgtaac tgttattacc caattgctga gatacgtttc cccagatgct taggttatct
     1021 gcaagacgcc cccccacacc agttttgagt tcaatcacat tacggttgcc tatctggtga
     1081 tttgacatgc cattcataat tacaccatat tgctttgagc tccatttcca attcacttcc
     1141 aaaaatggtt cgaacttacg aagagtctcc ttatcgatac aactctgact aattaaggat
     1201 gtacgcaatc ccaattttgc ttgaatatta ttgcccccag aggatgtgac catagtgcca
     1261 ttgtgttcaa tatagtcgtt tgcttttacc ccatgcctag ttatctggac ttgtggattg
     1321 agatacaatg cgttgtcaat attattatga gcagtccagc gtatggttgg gtagatatag
     1381 ccagcttcca aagctcctgt tattcctttg ctggaatatt tctcaccagt taacccatct
     1441 cctttgactg tattattaaa ccaactgtac tgcaaccaag catccatata tagacctgtt
     1501 cgttcttttt catcctgaaa ccaagaactg tacaaaccgg cagtataacc gctcacagtt
     1561 cctcgtgaac tataactagt atacttatta tgagttttgc tatcctgata tccataagct
     1621 cccataatac ccacatgcaa gccatcatga tcagtgaagt ttgtcttcaa aatatctcca
     1681 ccaatctgat aaaccatcct gttagctgtg gtttttaatt gtctatcagc taaattatgc
     1741 tcattatgtc caccaacagt acgaatccac atggtggttt cgctgagctg acctgtagta
     1801 ggatcaacaa tagtgctacc gtgtcggtca tagagagaag gactaaacat cgtgttcatt
     1861 gctatcaggt tagcaatata actgccagct tcagggcgcc atgtgtgaat actaccttca
     1921 cccaaattaa gtgaagatac aagctgtgaa ctagctcggc gactactcat ttgagtagac
     1981 tcttgattgc tcatctgttt tgattcttga ttatccatct gactagttag ataccactta
     2041 ttggtattca gtccagatac agtgccctgt ttcaggcgat agtcataact accagcaaca
     2101 atacggcctt tctgaataaa agcattctta tcagaagaat ctgtcgaaat aatttgaaca
     2161 ccttcaagag ttttatctcc agagcctccc tcatttgtca ctgcaacata ggtaatacca
     2221 gacgcatggc ctgcaatttc cagccggtca gttttactgt tgtcattgcc gagaaccgcg
     2281 cccaaagata atattccgcc ctttccatgc caattacctt tctgaacata ggtctgaccg
     2341 acatttgacg aactattatt gagaataaca tgaccgtttt tctccagagt catgttacct
     2401 gtgacaatga cggggtcagg caaaaaaggg gcattaatat tattgattag cacagtccca
     2461 ctatttaata aagtgttaac agtttggttc atgcctgaaa aattcaaggt agcaccttta
     2521 ttaacaataa caccagcggt acgtgtcata gcttcaactg tccccatttt tagaatgcct
     2581 gcattgatgt tggtgaaacc agagtaggta tttttttccg ccaaaatgag agttccctcc
     2641 cctgattttg ttagggattt tccgtcccag ctatttatat tatgatggct gagattatct
     2701 gccagaatag tactaacagt aagttcagcg ttttctttca gattgaactc tccatgactg
     2761 tcgccatctg tatcattcca gctcaaaccg tagatcaatt tattctggtc actgatatag
     2821 ctttttgtat agtcaatagc accagtagtg agtaacggat tgatgataga tatattagaa
     2881 acactgaaac ctgttatccc tttatctgca cttagcagtt tatagtcctt ctgcgcccag
     2941 ttatttataa tagcctccac ttttacattg ttgccaaatg acatatttcc agaagcttcc
     3001 agactactga ttccagctaa atttaaagaa ctgttctcac caaaagaaac gtcacctgaa
     3061 acagtgaatt tgttacctga tagtgaaaca gttgtacaat caccagcaca taaatcacct
     3121 tcaatagttg gtgtggtccc agtgtaatca ttgttgataa ttttcagaat ggatgaattg
     3181 ttaacctgta catcaccagt aatgacagaa ccttcatgga gtattaatga attgtttcca
     3241 ccagtgatgt ggatagcatt acctgctaca ttttgtgttc catcatcttc tttacctgaa
     3301 attatatatc cgctattagc aatggtcata ttgctaccat ttatagcatc cccccaatgt
     3361 ccaccattac cgcctaaaat agctccattg ttgattacag acaggttatc tcctgtgata
     3421 gcattaccac cgtagccatc ataaccataa ccgtaagcag cacctccgtt gcctccagcg
     3481 aacgtgccct tattgattac agagaggctg cttcctgtga tggcatcacc tccattaccg
     3541 ccatcaccct caccataatt gttacctcca ttaccaccgg aaatagtccc tttgttgatt
     3601 atgggtaaat tgactcctgt gacggcatca cctccattcc ccccatcact atcactataa
     3661 ctatcgccac catgtccacc tgaaataatt tctccattta ttataaacag attgtctcct
     3721 gtaacagcct caccaccatc accgttatgg tcagcaccgc taccgccgct accaccaaga
     3781 atcatgcctt gattgattat agacaagtca gatccggtaa tagagtcacc accattaccg
     3841 ccacaactat taccatcatt attatcacca gcaccaccat gacctgcgct aatcataata
     3901 tcagagttaa tccgtagctt agttctagat gcatgagagg ggttggaaga agttatatac
     3961 cacgcatcca ttccatcttc accagctccc aagggagaaa gtccatcaac aggtgttaat
     4021 aatttttcat aattgtcctc tgaaaaatga agttcttgag tacccgaaag aggagtagca
     4081 aaagctattg gccccccgag caacagggat gcaccaacaa caaaaggaga aaaagacaat
     4141 ttgcatgttt tttcctttac cgtaggtaat tctccggccc ccccctgtga acattgggtc
     4201 atattacaaa aaaatttgtg aatttgattc atgcactata ttatcagtaa gtggttgata
     4261 aacccctgaa aagattccgt tttatccgga ataaagggac gatatatgca aaaacatatt
     4321 aaacaaagcc tcaaccaaaa catgagagct gtgttatata tcaatgcaac gggagaaatt
     4381 tacctgtgtc ttgctttgaa aaaaagaggg ggaaagctat atgttcgata ataaattaat
     4441 aattttacta tgatagtctg cttcagaccg caacttttca gttctttctg tacgcttgcc
     4501 caaagtttaa ccactttgtt tttctataca cccaatggtt ctatctgccc cttgatgtta
     4561 atccccaaag ccaataacat gaatgtattc atatcacaac tttcctacca gactctccaa
     4621 tgcaataact gcataattgt ttttagtgga aatgtatcgt tttcttaaag agaagaataa
     4681 cattccattt tattatgttc ccaatttaat tatattaata tatattggca ttaataggaa
     4741 aatacatcag gagaaatcaa atgcagacat caaacataac taaccatgaa agaaatgact
     4801 catcatggat gtcaactgtc aaatcaacta cagaagtaag ttggaataaa ttgagttttt
     4861 gtgacatttt actgaaaata ataactttcg gcatatattc tccacacgaa actctagccg
     4921 aaaaacatag cgaaaaaaaa ctaatggata gtttctcacc atcactatca caagataaaa
     4981 tggacggaga gtttgcccac gcaaacatag atggcatttc aatacggcta tgccttaaca
     5041 aaggcatatg ttcggttttt tatcttgatg gagataaaat acaatccact caactttcat
     5101 caaaagaata taataacttg ttaagttcac tcccccccaa acaattcaac ttaggaaaag
     5161 tacatactat tacagctccg gtatcaggga attttaaaac tcacaagcct gcaccagaag
     5221 ttattgaaac agccattaat tgctgtacat cgataatacc aaacgatgat tacttccacg
     5281 ttaaagacac tgattttaat agtgtttggc atgatattta cagagatatt cgagcatctg
     5341 acagcaatag tacaaaaata tatttcaaca atattgaaat accactaaaa ttaattgcag
     5401 atctaattaa tgagttagga attaatgaat tcattgatag caaaaaagag cttcaaatgc
     5461 taagttacaa ccaagtaaat aaaataatca attcaaactt cccccaacaa gacctatgtt
     5521 ttcaaacaga aaaacttttg tttacttcac tctttcaaga ccctgcattt atatctgcac
     5581 taacatctgc attctggcaa tctcttcaca tcacgtcttc ctctgtggaa cacatatatg
     5641 cccaaattat gtccgaaaac atagaaaaca ggcttaattt catgcctgaa caacgagtta
     5701 ttaacaattg tggacatatt ataaaaatca acgcagttgt ccctaaaaac gacactgcaa
     5761 tctctgcctc tggggggagg gcttacgaag ttagctcatc aatattacca tcgcacataa
     5821 catgtaatgg tgttggtatt aataaaatag aaacaagcta tctcgttcat gctggaacac
     5881 ttccaagcag tgaagggctt cgcaatgcta tccctccaga aagtcgtcaa gtatcatttg
     5941 ccatcatatc tcctgatgtt taagccaata ttatattggc tgccagactg taaaaaaacg
     6001 ttcttcagaa cagctctata aactattaaa ccaaagctaa aaggatgggg agcaatagaa
     6061 tctcagccca tcctttttat atcattatga accgccccgg aaatcctgga gactaaactc
     6121 cctgagaaag aggtaaacag gatgactaaa aatacacgtt tttcccctga agtccgtcaa
     6181 cgggcagttc gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcagcgatt
     6241 tgttccattg ccccaaagat tggctgtata ctggaaactc tgcgtgtctg ggtacgccag
     6301 catgagcggg ataccggggg cggtgagg
//
LOCUS       DBBBSB010000183         6058 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.186, whole genome shotgun sequence.
ACCESSION   DBBBSB010000183 DBBBSB010000000
VERSION     DBBBSB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.186"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..143)
                     /locus_tag="ACIUS0_003899"
     CDS             complement(<1..143)
                     /locus_tag="ACIUS0_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HGB0221442.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACIUS0_003900"
     CDS             379..651
                     /locus_tag="ACIUS0_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGB0221443.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACIUS0_003901"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACIUS0_003901"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGB0221444.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="ACIUS0_003902"
     CDS             1111..1731
                     /locus_tag="ACIUS0_003902"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGB0221445.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACIUS0_003903"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="ACIUS0_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="ACIUS0_003904"
     CDS             complement(3114..3767)
                     /locus_tag="ACIUS0_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGB0221446.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="ACIUS0_003905"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="ACIUS0_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5241..6058)
                     /locus_tag="ACIUS0_003906"
                     /pseudo
     CDS             complement(5241..6058)
                     /locus_tag="ACIUS0_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1428 a         1621 c         1598 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttattccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DBBBSB010000184         6053 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.187, whole genome shotgun sequence.
ACCESSION   DBBBSB010000184 DBBBSB010000000
VERSION     DBBBSB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.187"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            430..1338
                     /locus_tag="ACIUS0_003907"
     CDS             430..1338
                     /locus_tag="ACIUS0_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HGB0221447.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACIUS0_003908"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="ACIUS0_003908"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGB0221448.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="ACIUS0_003909"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="ACIUS0_003909"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGB0221449.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACIUS0_003910"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="ACIUS0_003910"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGB0221450.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6053)
                     /locus_tag="ACIUS0_003911"
     CDS             complement(5673..>6053)
                     /locus_tag="ACIUS0_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221451.1"
                     /translation="VGMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIV
                     NAGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQN
                     ALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1577 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc gac
//
LOCUS       DBBBSB010000185         5808 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.188, whole genome shotgun sequence.
ACCESSION   DBBBSB010000185 DBBBSB010000000
VERSION     DBBBSB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.188"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            66..584
                     /gene="mddA"
                     /locus_tag="ACIUS0_003912"
     CDS             66..584
                     /gene="mddA"
                     /locus_tag="ACIUS0_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGB0221452.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            581..1030
                     /locus_tag="ACIUS0_003913"
     CDS             581..1030
                     /locus_tag="ACIUS0_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGB0221453.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1031..1264)
                     /gene="ydcY"
                     /locus_tag="ACIUS0_003914"
     CDS             complement(1031..1264)
                     /gene="ydcY"
                     /locus_tag="ACIUS0_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGB0221454.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="ACIUS0_003915"
     CDS             complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="ACIUS0_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGB0221455.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="ACIUS0_003916"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="ACIUS0_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGB0221456.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="ACIUS0_003917"
     CDS             complement(2215..3024)
                     /locus_tag="ACIUS0_003917"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGB0221457.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4658)
                     /gene="patD"
                     /locus_tag="ACIUS0_003918"
     CDS             complement(3234..4658)
                     /gene="patD"
                     /locus_tag="ACIUS0_003918"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGB0221458.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4680..5474)
                     /locus_tag="ACIUS0_003919"
     CDS             complement(4680..5474)
                     /locus_tag="ACIUS0_003919"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0221459.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5464..>5808)
                     /locus_tag="ACIUS0_003920"
     CDS             complement(5464..>5808)
                     /locus_tag="ACIUS0_003920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGB0221460.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1475 c         1288 g         1523 t
ORIGIN      
        1 cgttgcttcg ggctgttttc gatagtattg ataacatcat catcattatt ttattgaggc
       61 cagacatgtc catccgtttt gcccgcaaag ccgactgtgc tgccattgcg gaaatttata
      121 accacgccgt gttgtatacg gcggctatct ggaatgacca aacggtggat gcagataacc
      181 gcattgcctg gtttgaagcg cggaccatag caggttatcc ggtgctggta agtgaagaag
      241 atggcgtagt tacgggatat gcctcgtttg gcgactggcg tagtttcgat ggttttcgcc
      301 ataccgtgga acattcggtt tatgtccatc ccgatcatca gggcaaaggt ctggggcgta
      361 aattgttaag tcgattgatt gatgaagcgc gggattgcgg gaagcatgtc atggtcgccg
      421 ggatcgaatc gcaaaatcag gcttcgctgc atctgcacca gtcgctggga tttgtcgtca
      481 ccgcacaaat gccgcaggta ggcactaaat ttggtcattg gctggatctg acatttatgc
      541 agttgcaact cgacgagcgc actgaaccgg acgcgattgg atgaatcagt cgctcaccct
      601 tgcttttttg attgccgccg gaattggtct ggtagtccag aacacgctga tggcgcgcat
      661 cacccagacc tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt
      721 gctctttgtt tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc
      781 cagcgtgcgc tggtggacac ttatacctgg cttgctgggt tcgttttttg tcttcgccag
      841 tatcagcgga tatcagaatg tgggagcggc gacaactatt gcggtactgg tcgccagcca
      901 gctgattggc ggtctggtgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt
      961 gttcggccca atatgtggcg caattttact tgtggttggc gcctggttgg tggccagacg
     1021 ctcattttaa tcagaggatg gtgccgcctt tggttaactg ttcgtggcgc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaaa catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tattattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaattcctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaagtat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcacgatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcagcaccca gtttttccac cagcgtatcg
     3781 taaatgcctt tttgcgcgta gatccgacaa gccgcagtac aatcctgtcc agcattgtaa
     3841 tagccaaatg tacgtacacc ttcgaccact gcttcaatat ccgcatcatc aaaaacaatc
     3901 actggcgctt tgccaccaag ttccatatga gtacgcttaa tggacgacgc ggtatggctg
     3961 atgatgtgct cgccggtggc gatagagccc gtcagcgaca ccatccgcac tttgggatga
     4021 ccggtcagcg gatcacccac cgttttgcct ctgccaaaca gtatgttaat cacgcctgcc
     4081 gggaagatat ctttcgccag ctctgccaac ttcaacgcgg tcagcggggt aatttctgat
     4141 ggtttaagca ctacgcagtt ccctgccgcc agcgccggag caagtttcca cgcggccatc
     4201 atcagcggat aattccacgg tgcgatagaa gccacgaccc ccaacggatc gcgacggatc
     4261 atcgaagtat gaccttcaag atattcacct gccgccagac cattcagaca gcgcgccgca
     4321 cccgcgaaaa agcgaaaaac atcgacaatc gccgggattt catcattgaa cgcactatgc
     4381 agcggtttgc cacaattacg ggattccagt tcggcaaaaa cctgaccatt ttcttcgata
     4441 acatccgcca gtttcagcag gcattccgca cgcgctttcg gtgtggtttg cccccattca
     4501 gcaaatgctg catctgccgc acgcacagca gcatcgacct gctctgcgga tacctcggca
     4561 atctccagta aaacgtcccc cgttgccgga ttatagacag gctgtttttc cccttcgccg
     4621 ctaacccgtt ctccgttaat cagtaactta tgttgcatag cttattcctg tatcagtggt
     4681 tattgaccat tgtcgccttc gcgggttagc caccaggccc ccaggatcgg caaggttgtt
     4741 accaacataa ccagcagtgc caccacgttg gttaccggta catcacgcgg tcgcccaagc
     4801 tgattgagca accacaacgg taacgttcgt tcatgacctg ccgtaaaggt cgtaacgatg
     4861 atttcatcga acgacaaggc aaacgccagc attcctcctg ccagtaacgc cgaactgaga
     4921 ttcggcaaca ctacgtagcg gaaggtttgc cagccattgg ccccaagatc cattgacgcc
     4981 tcaaccagac tccacgaggt gcggcgaaaa cgggcgatga cattgttaaa caccaccact
     5041 acgcaaaaag tcgcatgacc gaccacgatg gtgaaaaatc ccggctccag attgatggtt
     5101 ttaaaggcgg ttaatagcgc cagaccagtg acaatgcccg gcagcgcaat gggcagcagc
     5161 aataacagcg aaatggcgtt tttgccgaaa aagtctcgtc tccacagcgc ggcagctgcc
     5221 agcgtcccta acaccagcgc aattaatgtc gccagcgccg ccactttaag tgacagcgtc
     5281 acggcatcaa gaatatcact acgctgtgct gccacgctaa accagcgcag cgtcaggccc
     5341 tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg cataggcggc gattatcagg
     5401 atgggaaaat gtaggaaaac aacgccgccc caggccgcca gtttgaggaa aaacggtgcg
     5461 cgttcagagt gcatcgaacg ctcccagacg tttcacgaac gccaggtaca gtgcgatgag
     5521 aataatcggc accagggtga atgccgccgc catcggcata ttgccaatcg ccccctgctg
     5581 ggaataaacc atattgccga taaaatatcc tggaggacca accagctgcg ggacgataaa
     5641 atcgcccagt gtgagtgaga aggtaaagat agagccagcg gcaatacccg ggattgccag
     5701 cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg ccgagatcag ccgacgcctg
     5761 caacaatgac ggcggcaaac gctcaagcgc cgcctgaacg ggcaggat
//
LOCUS       DBBBSB010000186         5803 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.189, whole genome shotgun sequence.
ACCESSION   DBBBSB010000186 DBBBSB010000000
VERSION     DBBBSB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.189"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            231..1163
                     /gene="rfaD"
                     /locus_tag="ACIUS0_003921"
     CDS             231..1163
                     /gene="rfaD"
                     /locus_tag="ACIUS0_003921"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGB0221461.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1173..2219
                     /gene="rfaF"
                     /locus_tag="ACIUS0_003922"
     CDS             1173..2219
                     /gene="rfaF"
                     /locus_tag="ACIUS0_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGB0221462.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2223..3215
                     /gene="rfaC"
                     /locus_tag="ACIUS0_003923"
     CDS             2223..3215
                     /gene="rfaC"
                     /locus_tag="ACIUS0_003923"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGB0221463.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3212..4420
                     /gene="rfaL"
                     /locus_tag="ACIUS0_003924"
     CDS             3212..4420
                     /gene="rfaL"
                     /locus_tag="ACIUS0_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGB0221464.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4456..5598)
                     /locus_tag="ACIUS0_003925"
     CDS             complement(4456..5598)
                     /locus_tag="ACIUS0_003925"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGB0221465.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5607..>5803)
                     /locus_tag="ACIUS0_003926"
     CDS             complement(5607..>5803)
                     /locus_tag="ACIUS0_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HGB0221466.1"
                     /translation="WHDWANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLL
                     YQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1609 a         1258 c         1293 g         1643 t
ORIGIN      
        1 attcaaatga ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca
       61 tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta
      121 gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag
      181 acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg
      241 ttaccggcgg cgcgggcttt atcggcagca acatcgttaa ggccctgaat gataaaggca
      301 tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg
      361 atctggatat cgcggactat atggataagg aagacttcct gatccagatt atggctggcg
      421 aagagttcgg tgatgtcgaa gcgattttcc acgaaggcgc atgctcttcc accaccgagt
      481 gggatggcaa gtatatgatg gataacaact atcaatactc caaagagctg ctgcactact
      541 gtctggagcg cgaaatcccg ttcctgtatg cctcttccgc agccacctac ggcggacgca
      601 cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaatgtctac ggttactcaa
      661 aattcctgtt tgatgaatat gttcgtcaaa tcctgccaga agcgaactcg cagattgttg
      721 gcttccgcta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg
      781 tcgctttcca tctcaacacc cagcttaata acggtgaatc accgaagctg ttcgaaggta
      841 gcgagaactt caaacgtgac ttcgtctatg tgggcgacgt ggcagatgta aacctgtggt
      901 tcctggaaaa tggcgtttcc ggtatcttca acctcggcac cggtcgtgca gaatccttcc
      961 aggcagtagc agatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt
     1021 tcccggataa gctgaaaggc cgctaccagg cgttcactca ggcagatctg acaaatctgc
     1081 gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg
     1141 cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt
     1201 gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc
     1261 aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg
     1321 aagttaacga agctatccct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc
     1381 gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc ttaccaaact
     1441 ccttcaaatc tgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg
     1501 gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct cgataaagaa gcctggccgc
     1561 taatggtgga acgctatgtc gcgctggcct atgacaaagg cattatgcgt accgcacaag
     1621 atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata
     1681 cctgtaatca attttcgctt tcatcagaac gtccgatgat tggcttttgc ccgggtgcgg
     1741 agtttggtcc ggcaaaacgc tggccacact accactatgc ggaactggca aagcagctga
     1801 ttgatgaagg ttatcaggtg gttctgtttg gctctgcgaa agatcatgaa gcgggcaatg
     1861 agattcttgc cgctttgaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa
     1921 cacagcttga tcaagcggtt atcttgattg cagcctgtaa agccattgtc actaacgatt
     1981 ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccaa
     2041 gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg
     2101 gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca
     2161 ttactcccca gcgcgtactg gaagaactca acgcgctatt gttacaagag gaagcctgac
     2221 ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg
     2281 cactcactga tgcccagcag gcaatcccag ggattaagtt tgactgggtg gtggaagaag
     2341 ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac
     2401 gtcgctggcg taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg
     2461 aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg
     2521 cggcactggt gacacgtctg gcgcatggcg taaagcatgg attggactgg caaaccgctc
     2581 gcgaaccttt agccagcctg ttttacaatt gtaagcatca tattgcaaaa cagcagcacg
     2641 ccgtagaacg cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc
     2701 agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat
     2761 atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc
     2821 gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc
     2881 cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc
     2941 cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg
     3001 tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg
     3061 gaccaaccga tccgggatta attggtgggt atgggaagaa tcaagtagag tgtcgttcta
     3121 caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa
     3181 taataaccaa taagttgaca tcggagataa gatgacctca acattatttt tctctctcga
     3241 gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta
     3301 ttttttgggt gggattacaa ggtataaaca tcttattgtt attcttatga caataacgac
     3361 aatcgtctat ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa catttctttt
     3421 tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat cccctgatgc
     3481 aggtgctagc atgaaggaag ttttcaaagc tattattgag aatactttac tatgcacaat
     3541 agcaataccg gtcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt
     3601 ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga
     3661 ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat
     3721 ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat
     3781 ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg
     3841 ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa
     3901 gttactatta gtaggagtaa tggtttccat cattgcattg tcggttattt tcacacataa
     3961 ggagatgacc gcaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc
     4021 aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg
     4081 ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg
     4141 gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg
     4201 tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag agtgtataaa
     4261 aaatggcgtc aagaataaat atcgctcacc atataatgca tattatataa tcttactatc
     4321 ttttataggt tattttgtta tccgtggaaa cgtagaacaa attgaaccaa atttattagg
     4381 cgtttacgcc ggcttattat tagcgatgaa aaacaagtaa aaaataaaaa aggctgcata
     4441 atgcggcctt tttatttatt caaaccaatt atgaataacc tcttcgaatc tttgagttac
     4501 gccatcccaa ctgtagtgat caaacacaaa gtcctgacct tgctttgcaa ctgctgttaa
     4561 ttcaggattc gctaacgttt ttaaaatatc gctgcttatg gagtctgcag tcataggttc
     4621 ttttaaatgg aatcccgtag tattttcttt gacaaattcc gtcatccccc cacgcgtact
     4681 cactaaaaca ggcttaccag cacccatagc ttcaattgcc accatgcaaa atggttcctg
     4741 gaactgggaa ggaatgacga ctaaatctgc cagtgggtaa taacaataca tcttttcagg
     4801 cggtatgctt cctaacataa tgcaacgatc cttcaaacgt tttgcaattt ccctgacatt
     4861 acgttgatat gcacctttgt cacttttact catttctgta taatcaccaa tcacaactaa
     4921 ttttaaattt ttgtgagcag cggccaattt ttcgaatgat tgcatcaaca gcaatatgcc
     4981 tttgtcaggg acgatacgcc cagcataaaa aataattttc tcttcaggag agatattaag
     5041 ttcgcttcta gtgatcggct gaaaattaga ttgataggtt tctaaatcaa ttccatttgg
     5101 tacaatctca atatctgcat ttgctaaata agattggtaa tactttttca agtataaact
     5161 agggactatc attttaacat tttgctcaag caattttggt tcaaatgcat tatgcatatg
     5221 tataaccact cttgcctgag gagctagctt acgaatttga gtgtatagct tcatgctatt
     5281 atgaactaca ataacacttt ctttcgttat aggaaaatca tgtgcaatat tcaatattct
     5341 ctgtgaatat ggaagaggat caagtcttgt ccatttttga aataaccgct tatatatgcg
     5401 actgaatcca accctatgaa tactacaatt atcattaaca aaggtgtaat ttgagtatcc
     5461 tggatttttg atacaaacaa tacgatttgg aaaattggta cgctgagcaa cctgatacat
     5521 ccacgtttca acagcagccg ctccgcgagg aggaattgaa aaaataggag taaccgtaaa
     5581 tattatttta tcaaccatct cgtatattaa cctttcataa cattatattt aattgctgta
     5641 aaaacaccat cgagaaattt tttctggtaa agcaagtgtt tatatttttc tttgtgctca
     5701 tgttttttaa cagctttttt ataaggtata tttctccatg gtgatatatt ataaatatta
     5761 cgaaaataat ctgcagaggc gtagttcgcc caatcatgcc atg
//
LOCUS       DBBBSB010000187         5743 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.190, whole genome shotgun sequence.
ACCESSION   DBBBSB010000187 DBBBSB010000000
VERSION     DBBBSB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.190"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..172
                     /locus_tag="ACIUS0_003927"
                     /pseudo
     CDS             1..172
                     /locus_tag="ACIUS0_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(488..826)
                     /locus_tag="ACIUS0_003928"
     CDS             complement(488..826)
                     /locus_tag="ACIUS0_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221467.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            748..1875
                     /gene="ltrA"
                     /locus_tag="ACIUS0_003929"
     CDS             748..1875
                     /gene="ltrA"
                     /locus_tag="ACIUS0_003929"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGB0221468.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            1958..2137
                     /locus_tag="ACIUS0_003930"
                     /pseudo
     CDS             1958..2137
                     /locus_tag="ACIUS0_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2164..2649)
                     /locus_tag="ACIUS0_003931"
     CDS             complement(2164..2649)
                     /locus_tag="ACIUS0_003931"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGB0221469.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2905..3885)
                     /locus_tag="ACIUS0_003932"
     CDS             complement(2905..3885)
                     /locus_tag="ACIUS0_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HGB0221470.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3885..5084)
                     /locus_tag="ACIUS0_003933"
     CDS             complement(3885..5084)
                     /locus_tag="ACIUS0_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGB0221471.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5258..5392)
                     /locus_tag="ACIUS0_003934"
                     /pseudo
     CDS             complement(5258..5392)
                     /locus_tag="ACIUS0_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5406..>5743)
                     /locus_tag="ACIUS0_003935"
     CDS             complement(5406..>5743)
                     /locus_tag="ACIUS0_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221472.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1484 t
ORIGIN      
        1 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
       61 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
      121 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa ttgcagcata
      181 agttatccat gctctgaggt gggtagaggt gggccctccg gtcagggttt acaggagcac
      241 agaccaaacg acccggtgct gcttcattct aaggaatgag gtggtgagcg actcgggaaa
      301 aggcagagat ctactgtcac gtgggttcgg gaagctgaac gaaagtaaac tgtccgatga
      361 agcatcgaaa tcttagatga tgccaaaatg gggccgtaca gccgtcccct gataagcgca
      421 gaagacacct gcttactgac tgcgcggcat ccggtgtgaa ggcagcagga tcccgggcca
      481 gaccatctca gggaacgtga gaacctgttt ccggcacgta tcgggaaaca ggggcggacc
      541 cattcgtagt agtgatgaaa gatctgtaat gggtctggag cgaagggatg gggatcacat
      601 tcattgcaga agacacaacc caaaagggga tgacgtaact gatgcaacga aaatcatttg
      661 aaataccaaa agcactggtc tgggcatcat atctggatgt gcgtcgcaac aagggagccc
      721 cccggatgcg acgggcagac tttgaaaatg ttcgaccagc aacgtgatgg caatctatac
      781 aagatctgga accggctatg ttcgggaaca tggtttccgc caccagtgct ggaaaaacgg
      841 atcccgaagt cgaatggcaa ggaacgcatt ctggggatcc caacagtatc cgatcgaatt
      901 gctcagggag cgataaaact tttcatggaa gaaaagcttg acccgatttt ccatgctgat
      961 tcatatggtt accgaccagg caaatcagct catgacgctc tgaaacaatg tgccatccgg
     1021 tgctggcgtt atagctggat actggaagtg gacatcagtg cgtttttcga ccatgtgaga
     1081 catgatctgg tgctcaaagc tctggaacac cacgggatgc ctaaatgggt catcctgtac
     1141 tgtcgacgct ggatggaagc gccaatgcag agttgtgaaa atggagaatt aataacccga
     1201 acacgaggca ctccgcaggg cggggtcatt agtccgctac ttgctaactt gttccttcac
     1261 tatgcatttg atttgtggat ggaaagagaa tatcgggggg taccgtttga gaggtacgct
     1321 gacgatattg tagtacattg ttcacgaatg agtgatgcaa caaggctaaa gaacaggtta
     1381 tcggagcgct tctcggaagt cgggctggtc ctgaatgcag ggaaaacgaa tatcgcctac
     1441 attgacacgt ttaaaaggag aaacgtcgca acgagtttca cctttctcgg atatgacttc
     1501 aaagtgcgta cgctgaagaa tttcaacggc gaacggtacc gaaaatgcat gccgggtgcg
     1561 tcaaatgcag caatgcgcaa aataacagaa acaatcaaga agtggcgtat acatcgctca
     1621 acagctgaga gtttgctgga ttttgcgagg cgctacaatg cgatagtgag aggctggatc
     1681 gagtactacg gaaagttctg gtccagaaat ttcaactatc gactgtggag tgcaatgcag
     1741 tcacgtctgc tcaagtggat gcagtctaaa tacagacttt cgaaccggaa ggctcagcga
     1801 aagctgacgc tggtaaggaa ggagtatccg aagctatttg ttcactggta tttactacgt
     1861 gcatcaaatg agtgatcaag agccgtatga cgcgagagtg tcacgtacgg ttccgtgagc
     1921 agccggggag tgaaattctc tccggctgac tcgactcgcg ggttccgaca gcgatgacga
     1981 acatgcggcg gtgttgtact cgctgatcgg cacatgccgt ctgaacaatg tggaaccaga
     2041 aaaatggctg cgttacgtca ttgagcatat ccaggactgg ccggcaaacc gggtacgtga
     2101 tctgttgccc tggaaagttg atctgagctc tcagtaaata tcaatacggt tctgacgagc
     2161 cgcttacttt tggcgtaatt ctgctgcgcc tttactgaac acaatgccgt attcagcaag
     2221 aagtccacga atgtgattta tcagttgtgt cctttccatt gaaaccaacc gacgcatatt
     2281 caaaaaagtc tggacgtcct gttgctccag agtttttatt ggtacgggtc gtatagttcc
     2341 atgttggcag gcctcagcta ttgcctgtgc gtcgttataa tcatttttct ggcaccgctg
     2401 ataagcttta acatgttgtg ccggaagcaa tattacatca tggtcgagtt tcctgatttc
     2461 ccgggcccag taatgagatg ccccgccagc ctccatcgca acagtcattt taggctgttg
     2521 caccagccag ttaagcagtt gattgcgaga aagtttggtc tttcctgcgg gtttgccatg
     2581 agcattgata ctgaaaagat aaaagttcgt tttagccaga tcaatgccgg tgattttctg
     2641 ttcagacatg gtatcccgct tacttttgtt gttaaagttt atccatcatg gcgcaattga
     2701 aggctgccgt gaaagagggg gaagactatc tcatcggtta ctgttaaacg gctaagagaa
     2761 actatctttt tcaagcaaag aagatacggt gaaacaggcg atttcaccgt gaaatactgt
     2821 tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac agccaattaa aaactggcga
     2881 gttgctggcg aaaaacttga attttcacag cgaattcaga ttatctttta gtacaagagc
     2941 aatcatacga tctaacttat cctgaacttc caacggtaat ctgccgaatt catatgagaa
     3001 cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta ctatcaaatt tccataacaa
     3061 agttacaacc gctttatcct ttactcctga atcaagcaat gaagacattt ccttggttat
     3121 aagccgcata atactgtttt taacctcgtc ttcagaaaga ttgtcatcag cagtaataag
     3181 ctcaatactc ggcgagatat tcttcacaac ttcatcaata gatatatcac ccagcctcag
     3241 tcgttcactt aattcagcca gttgcctgta atcggcgtat gtcagctcag actgaacagg
     3301 aaaaagagac acaaaatctt taggtacgga cgctgcttgg agggcacgag tcaccttagc
     3361 tgccgacaat ccctctcttt ccgcaatttg cttctggttc ataccggcct ctttgagacg
     3421 tactaaacgc aacccaattt cacgaatatt atgttcaagt gacgtctgca aatctttcgc
     3481 cagatgctga gcttcagaaa cagtaagctc atcatccgtt acaagtacac gtaatccaac
     3541 cttgcatagc aaagctgctg cccgacgacg ggaaccatcc aaaatctcaa taagatcgcc
     3601 agtccgaatc ccaatacatg gatagaattg ttgtaaacgt atagttctgg tgatgtcttt
     3661 tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc acctcttcaa ccacaaaagt
     3721 ttgagtatcc actttgtcat gatcaacctt gatctcagta aattttgcct ttcggccagt
     3781 attgagggta aatacatgaa ctggagccga tatttcttca gtattattca ggatatttgt
     3841 attcagcgta cggccaatag tcggtcggtg ctttctgttt tccatcagta gttggccctt
     3901 ataaattcaa tacgatcaaa aactgcttta gcgaaatctt ctgctgccat acgtgcattt
     3961 ttcaaagctt caccactgcc aacataagta acaggattcg cagatatcac ggtatcaaac
     4021 gactcgccgc ttctctcaaa accatcaaga cgtggcattg aaacatcaag catgtcacca
     4081 ccgaaaatct cttttgtcag gctatggcag tatttatgat ctgacttatt cgccagtttc
     4141 gacatgaagc caatatttgc ctgcaaacgg catgaacatc catcctgttc aataatctgt
     4201 acaagttcag gtaaacgagc caaatattta agcgtggaat ggaaatcaac ttgtgctggc
     4261 ggcaccggtg taaacatgat atctgcggca gcaatggcat ttttcaaaaa tgcgtccaga
     4321 tgcgggcctg tatcaataag gatgaagtcg aaatcatgct tcagcttatc aataatattc
     4381 tcacgcaata tcgcgtgttt gttttgtcct aatagatgct cctcgcacag agtatcccag
     4441 tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa cataaacgcc agggataact
     4501 gacggaacga taaaatcttc cagtaattcc tcacgactca cgttctgcaa catagcctgc
     4561 ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt tcagaaacat agtggctgat
     4621 gactggggat ctaaatcaag gagcagaata cgtaaatctt ctgacagcag gtgaggatga
     4681 gcacgcaggg cgtgagctac ggaaacacta ctcacggttt tagacacacc gccttttaga
     4741 ctaccgatga atatggaata tgcctcacta taacgatcgc gatatttagg aatgccacga
     4801 tgtgcataga tatcgataat gttctggatg gttaatgcat attttgtcgc ggttcctgct
     4861 gggcgttttt caaactgata gccctgagcc tccatctcac cgactgcacc atccacgctt
     4921 gcgcgcgtta attttggaag cttagctaca gcagactttg aatagacttg ataaaaaaca
     4981 tcagcctgaa actcctgctt ttgctcctga atctgttttg ttagggcgag caacatttta
     5041 tctgcacgct gcgcaacctt acttaactgc tcaaaactag tcattccccc tccgtgctat
     5101 gtaggatttt aacttattac aaaaaaaact gcattagcag ggtaatgcat gcaagtacat
     5161 attgtacatg cttatattaa aaatgcgcgc attgctgatt tattaatcat cggaatgaat
     5221 attactgtaa gatagtatgc ctttccggga gaaagacgct gatttccccc gaataatcca
     5281 gttcaccacc acactccgga caacagcttt cttcaggctc cagatggtgt atttcgcggg
     5341 aaacatgttc cggtaacgga cggcgatgac gtgactgtcg caacaggcgg gggtagtgct
     5401 tccaatcagt agaacatgtg cttttcaatg aaggagccga tgactttttc gtggatttcc
     5461 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggt gcggagggtc
     5521 cggttgttac gtgcaatacg ttgcgtgaag attttgccgg tcaggtgttt ctccttcggc
     5581 acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca gaacggagtc
     5641 aacagggcta acagttcccg acaggtctca tcattccgtg gcccgaaagt gtaagccaac
     5701 acaccgccag ttttagtgtt gtacgcatac cagagccagt gtt
//
LOCUS       DBBBSB010000188         5738 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.191, whole genome shotgun sequence.
ACCESSION   DBBBSB010000188 DBBBSB010000000
VERSION     DBBBSB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.191"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..241
                     /locus_tag="ACIUS0_003936"
     CDS             <1..241
                     /locus_tag="ACIUS0_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221473.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACIUS0_003937"
     CDS             complement(238..1179)
                     /locus_tag="ACIUS0_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HGB0221474.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACIUS0_003938"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACIUS0_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACIUS0_003939"
     CDS             complement(1684..2904)
                     /locus_tag="ACIUS0_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HGB0221475.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACIUS0_003940"
     CDS             complement(2908..3651)
                     /locus_tag="ACIUS0_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGB0221476.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACIUS0_003941"
     CDS             complement(3542..5008)
                     /locus_tag="ACIUS0_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HGB0221477.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACIUS0_003942"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACIUS0_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DBBBSB010000189         5702 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.192, whole genome shotgun sequence.
ACCESSION   DBBBSB010000189 DBBBSB010000000
VERSION     DBBBSB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.192"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..170)
                     /locus_tag="ACIUS0_003943"
     CDS             complement(<1..170)
                     /locus_tag="ACIUS0_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221478.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACIUS0_003944"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACIUS0_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGB0221479.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACIUS0_003945"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACIUS0_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACIUS0_003946"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACIUS0_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGB0221480.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACIUS0_003947"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACIUS0_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACIUS0_003948"
     CDS             5484..>5702
                     /locus_tag="ACIUS0_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HGB0221481.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DBBBSB010000190         5601 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.193, whole genome shotgun sequence.
ACCESSION   DBBBSB010000190 DBBBSB010000000
VERSION     DBBBSB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.193"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..411
                     /locus_tag="ACIUS0_003949"
     CDS             <1..411
                     /locus_tag="ACIUS0_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221482.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACIUS0_003950"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACIUS0_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACIUS0_003951"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACIUS0_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HGB0221483.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DBBBSB010000191         5590 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.194, whole genome shotgun sequence.
ACCESSION   DBBBSB010000191 DBBBSB010000000
VERSION     DBBBSB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.194"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..279)
                     /locus_tag="ACIUS0_003952"
     CDS             complement(1..279)
                     /locus_tag="ACIUS0_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221484.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACIUS0_003953"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACIUS0_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGB0221485.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACIUS0_003954"
     CDS             complement(1254..1670)
                     /locus_tag="ACIUS0_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221486.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACIUS0_003955"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACIUS0_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACIUS0_003956"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACIUS0_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACIUS0_003957"
     tRNA            complement(2915..3004)
                     /locus_tag="ACIUS0_003957"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACIUS0_003958"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACIUS0_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGB0221487.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACIUS0_003959"
     tRNA            3996..4071
                     /locus_tag="ACIUS0_003959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACIUS0_003960"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACIUS0_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1507 a         1326 c         1293 g         1464 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg tgagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DBBBSB010000192         5560 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.195, whole genome shotgun sequence.
ACCESSION   DBBBSB010000192 DBBBSB010000000
VERSION     DBBBSB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.195"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            268..591
                     /locus_tag="ACIUS0_003961"
     CDS             268..591
                     /locus_tag="ACIUS0_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221488.1"
                     /translation="MENYAKFVATEILKQLGGNRFIAMTGATKFAYFDENGECGLFFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFFRIRGTTKKEISKFDNIYCDQLESLFSEETG
                     LATRF"
     gene            945..1289
                     /locus_tag="ACIUS0_003962"
     CDS             945..1289
                     /locus_tag="ACIUS0_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGB0221489.1"
                     /translation="MPYSAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMS
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTVDLDKAVSYL
                     QDFKKRSKNDSH"
     gene            1276..1626
                     /locus_tag="ACIUS0_003963"
     CDS             1276..1626
                     /locus_tag="ACIUS0_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGB0221490.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
     gene            2460..2975
                     /locus_tag="ACIUS0_003964"
     CDS             2460..2975
                     /locus_tag="ACIUS0_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGB0221491.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSADEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
     gene            3079..3621
                     /locus_tag="ACIUS0_003965"
     CDS             3079..3621
                     /locus_tag="ACIUS0_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221492.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCPSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWSSPYHVCTLSRKRNPVDIAANIERKILLNASQEVL
                     QAIEYEKRQAAKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGERYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            3618..3872
                     /locus_tag="ACIUS0_003966"
     CDS             3618..3872
                     /locus_tag="ACIUS0_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221493.1"
                     /translation="MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTL
                     SELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYKESDA"
     gene            3869..4111
                     /locus_tag="ACIUS0_003967"
     CDS             3869..4111
                     /locus_tag="ACIUS0_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221494.1"
                     /translation="MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHN
                     QLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP"
     gene            4658..4951
                     /locus_tag="ACIUS0_003968"
     CDS             4658..4951
                     /locus_tag="ACIUS0_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221495.1"
                     /translation="MKFTSTTNHVFTFERVTLCTIVLIHKDTGQQYVVIFTDNNKIRD
                     YKTGIVSQFGELKQSDIDLILFYRDEYEKYFDSLNNGEEYLSFKEYIGCIRGK"
     gene            5258..5545
                     /locus_tag="ACIUS0_003969"
     CDS             5258..5545
                     /locus_tag="ACIUS0_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221496.1"
                     /translation="MINEAKKIAVEMMFWTACPVDDDNCFDAIINASKIDREFEDKHA
                     IYLHPIYRDLLSTDQIKSALKKRAIKIISFKCDINEDSARKILMQTILRAV"
BASE COUNT         1697 a         1039 c         1312 g         1512 t
ORIGIN      
        1 tagcaggggt ggaaggtggc ccaggttccg gttgataaca gtagcggcgt agccgcgctt
       61 cggggcgatg tgactcctgt gctcccctgg tattttcgtt cgccccttac atccgctggc
      121 gcggataaaa agcgtgacaa tcatcaaata atttaccgat tttcaggcca gaaaccttgc
      181 taatacatct aaatagatgt attgttattt catcgaaagg ggattggctc ctttccttct
      241 taagtccaaa cggacaggag attaaaaatg gaaaactacg ctaaatttgt tgctactgaa
      301 atactgaaac aactcggtgg aaatcgtttc atagcgatga caggggcaac aaaatttgct
      361 tatttcgatg aaaatggtga gtgtggcctt ttctttcgtt tgccttcaaa ctttgcaatg
      421 aagggcatta atttagttaa aatcaaatta acattttctg atacatactt ggtgacattt
      481 ttccgtatcc gtggaaccac aaaaaaagaa atatcaaaat ttgataacat ttattgtgat
      541 cagcttgaaa gtttgtttag cgaagaaacc ggacttgcaa ccagatttta attttaaata
      601 tggtctgatt gagtaacgga acaattaata caggtgggca accagcccac ctatcagtaa
      661 aaattaatat gaggataata atatgaaact taatattaat tttacagaga acggagtacg
      721 tggtaacagg ggcttaattg tcccttttta tccggtgcgc tatttgataa aatattaagt
      781 gtgtattaat acacattttt agggcgtgat ttctggttgt ttttgtaaaa gccaagttct
      841 atcacgccag actcacattt tgtcacttta aagagacatt cttcttgatt tgatgtcact
      901 tataagagac aatgtgcaca ggttaaggga atgggggcgg caagatgcct tactcggcga
      961 aacgatatca gacagaaaac ggtgaggttc catacaccga ctggatgaaa aaattaagac
     1021 gtaaagacca gaccgcagcg ttaaaggtgg attccagaat tgcccgcgct atgagcggaa
     1081 attttggaga tcataaattc gaaagggacg gtgtctggga attacgagtt gattacggtc
     1141 ctggttacag ggtttattat tcaatcgaag atggcgaaat aattcttctg ctcattggag
     1201 ggaataaaaa aactcagaca gtcgatttag ataaagccgt tagttatctg caagatttta
     1261 aaaagaggtc aaaaaatgac agccactaaa aatgtaaagt cgcagaaaaa tgattatccg
     1321 gcatccgttg atcacaatgc tgccatgatt gaagaattgc gcgcagatcc tgattatgca
     1381 aacgcttatc ttgctaatgc cctggaagaa ataaacgagc ctggtggttt aggcggtttt
     1441 cttgttgcgc tacgtcaggt aatcgaggcg cgagggggga tttccgaagc cgccaggaaa
     1501 tcggggcttg cccgtcagag tatttaccgc gcattatccc cgaacgggaa cccgaccatc
     1561 acaacacttg cacaacttac atctgtagcc ggattacagt tcactctcag taaatccagc
     1621 cactaacctc tccagttccc cctaagcggg ggaatttcct catgtcagca aaaaatcatc
     1681 aaacccgcag ttgtcccgac cgaagttcac tggtatttat cgcggggaat aatgccaccg
     1741 tacgagtgga aacatgtgta tttctgtgtg cccctgtata atccggtgat ttgtctgtga
     1801 ctggaacctt aaagctaatg tatgcataca ttagctttaa gcctgtattc ccgtcgttat
     1861 cgaggccgcc tggaaaacgg aataaaccac gttaagagta ttttttagcc acatggcttt
     1921 tgggaatcgc cagaatggcg ggataactgt ggttttggtg tctgaaatcg tacaattgta
     1981 tactggcaat agtttattgg tgtgtattaa tacatacctt aagtcgatgt gatttctggc
     2041 gcgttgcccc cgtagctcgc tggcgctcac acgcgcagta cagcgccccg tccagggaaa
     2101 ccctggacgg cgtaaactcg ctggcgcgag cgctaactgc ggcattctgc ggcgcaaatc
     2161 agcgttatcg ttgtctgtga gtcagtcggg cttagcaggg gtggaaggtg gccctggttc
     2221 cggttgataa cagtagcggc gtagccgcgc ttcggggcga tgtggctcct gtgctaccct
     2281 ggaattttcg ttcgcccctt acatccgctg gcgcggataa aaagtgtgac aatcatcaaa
     2341 taatttaccg tttttcaggg cagaagcctt gccaatacat ctaaatggat gtattattgt
     2401 ttcatcgaaa ggagataggc ttctttcctt cttaagtcca aacggacagg agatttaaaa
     2461 tgaatatcca ggaagcatta aacgtttttg gattatccgg cgaattaact gaaaaagata
     2521 tcaaagcagc atacagaaaa gccgctttaa aatatcatcc agatcgtaac ccgttagggg
     2581 ctgaactgat gaaagcggta aatgcagctt ttgatgtctt gatggcaaat attgataaaa
     2641 taaatcagtt ccagagcgct gatgaacatg cacgatataa ttacggtgat gacctggaaa
     2701 aagtattaaa cgttctttct ggtttatctg gtctggtatt tgaagtgata ggtaactggg
     2761 tatggattag tggagaaact attacacata aggaaacttt aaaagaaatc gggtgtaaat
     2821 gggcggcaaa gaaaaaacaa tggttttatc gtccagacga acataaaagt tactggaatc
     2881 gtgaagaaca cacgatagaa gaaatccgcg caaaatacgg tacaaccgga cagcgcaggg
     2941 cgacagggtg gcaacgcgtg gaaaccagag cgtaaccaga acgggggcga gaagcccccg
     3001 atatcatacc agcccgtctt ttggggcatc gaacccaaaa agacgggaac agcagccacg
     3061 tagcaaaaag ggaaacagat gaactctttt tttgaacaat atcaccctgt ctttgaagtt
     3121 gtctgccgta tcctggggaa cggctggcgc gtgaacaaac ttgatgattg tccgtctcgt
     3181 ataaaactga cgtcaccgca gtttaaaaat tactctgtgc atattcggat ggaaaaagac
     3241 cgattttctg ttgtggggag tgtggatagt cgttcctgga gtagtccgta tcatgtttgc
     3301 acgttatcca ggaagcggaa tcccgttgat atagcagcca atatcgagcg aaaaattttg
     3361 ctaaatgctt cgcaggaggt gttacaggcg attgagtatg agaaacgcca ggcggcaaaa
     3421 aaggacgaaa ttctgatcct gaaaggtatg ttatcgcaac ttgtccagct tgaaagctgg
     3481 tatggggcat tgacaggttt taaagctgaa aatggattga acggtaaagt caccgagcag
     3541 ggcgagcgtt atgatttgca gattaggggt ttgagtatag atcaacttgt taaaattaca
     3601 ggatatttga aacagttatg agaaaaaaca aagggcgatt gacttattat cttgaagtga
     3661 ttgataaaaa ataccatttt gtaaaaaaaa taagcagtta ttcaaaggag ttcactgacg
     3721 gaaaaacaaa aagaacaaag agaacgttaa gtgagctggt ttttaatgaa agtgaggtcg
     3781 aggcaataga ctttacaaaa aatggtttaa gacctgttga taagaatatt ctcttaacta
     3841 tggtgaaaga atataaggag agtgatgcat gactatcgaa aatacaccgg aaaacataaa
     3901 gaaattacgt aaaaaaattg gccttaccca gacagagtgc ggtgagattt ttggcgtagg
     3961 cttaagtaca tggcagaaaa aagaagctaa aacccacaac cagcttaatt tatcgaaagg
     4021 tgaatttgag tacctgttat tacttgcagg ggagcaccct gaatacgtcc tgcataaaaa
     4081 agctgaatcc cgctcaccgg aggaaccgtg agcgagaaga aaaacaccac gacgcagaaa
     4141 tctaagatat acagtgtaaa ggtttatttg tctttacaca gtgtgtgttt gtaacagggc
     4201 gactttacat aattgcgatt ttgcctggtg tgttgttgcg cccgtagctc gctgacgctc
     4261 acacgcgcag tacagcgccc cgccctgctg caatgcacca ggacggcgtg aactcgctgg
     4321 cgctcgcgct aactgcggca ttctgcggcg caaatcagca tgatcatagc ctgttggtct
     4381 gttctggctt tgcagggcga agggatggcc ctgtgcatcc tggtatgttc gtcagcggct
     4441 gcgccgcgct tcggggcgat gtggccccgc tgctccctgg tatttttttt cgccccttct
     4501 atccgctgtc gcggattaaa aaatgagcaa gccatcaaat aacttgctgt tttcaggcaa
     4561 taaatcttga taatacatct aaatagatgt attattattt catcgaaagg ggattggctc
     4621 ctttccttct taagtccaaa cggacaggag atttaaaatg aaattcactt caaccacgaa
     4681 tcatgttttc acattcgaac gagtaacgct ttgcacgatt gttcttattc ataaggatac
     4741 gggacaacag tatgttgtca tatttacgga taacaataaa attcgtgact ataagaccgg
     4801 aattgtttct cagtttggtg aacttaagca gagtgatatt gatttaattc ttttctatcg
     4861 ggacgaatat gaaaaatatt ttgactcgtt aaataatggc gaggagtatt tgagttttaa
     4921 agaatatatc ggatgtataa ggggaaaata aattatgaaa ggtgtgttca ggttgctgga
     4981 acaacctgaa cacgttatcg acaatcaaaa ctgaatgagg tttttaaatg tcaacacgac
     5041 agatcgtaca gcttgctgat tctcttgtca aaggctggga aaccagaatc ccggcaatga
     5101 cgtttagtga gtgggatcaa ttccggtggt ggttaaacta cctgcaaggg tatgaaatgt
     5161 tttaaataaa aaggggcatt tctgcccctt catatattta gacaaaaata ctatatgtaa
     5221 ctatagtcgt ctgcaagtaa taaagggggg gcaggggatg attaatgaag ctaaaaaaat
     5281 tgctgtggaa atgatgtttt ggacagcttg tcctgtggat gatgataatt gttttgatgc
     5341 tattattaat gcatcaaaaa ttgacagaga atttgaagat aaacatgcga tttatcttca
     5401 tccgatttac agggatttat tatcaactga tcaaataaaa agtgctctga aaaagagagc
     5461 aataaaaata atttccttca agtgtgatat taatgaggat tcggcaagaa aaattttaat
     5521 gcaaaccata ttaagagcgg tttaacttct ggtgtgttgc
//
LOCUS       DBBBSB010000193         5558 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.196, whole genome shotgun sequence.
ACCESSION   DBBBSB010000193 DBBBSB010000000
VERSION     DBBBSB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.196"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..642
                     /locus_tag="ACIUS0_003970"
     CDS             <1..642
                     /locus_tag="ACIUS0_003970"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HGB0221497.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACIUS0_003971"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="ACIUS0_003971"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGB0221498.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="ACIUS0_003972"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="ACIUS0_003972"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGB0221499.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagatc
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagatat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaa
//
LOCUS       DBBBSB010000194         5492 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.197, whole genome shotgun sequence.
ACCESSION   DBBBSB010000194 DBBBSB010000000
VERSION     DBBBSB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.197"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            31..1050
                     /locus_tag="ACIUS0_003973"
     CDS             31..1050
                     /locus_tag="ACIUS0_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0221500.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            1160..2032
                     /locus_tag="ACIUS0_003974"
     CDS             1160..2032
                     /locus_tag="ACIUS0_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGB0221501.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2366..5488
                     /gene="agn43"
                     /locus_tag="ACIUS0_003975"
                     /gene_synonym="flu"
     CDS             2366..5488
                     /gene="agn43"
                     /locus_tag="ACIUS0_003975"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGB0221502.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
BASE COUNT         1414 a         1414 c         1495 g         1169 t
ORIGIN      
        1 gttataagag aggcaccatg gagtgagata atgaataacg aacagatgct tatcaaatgc
       61 agagatcccc gtaaatgtcg gttttatgcc gaatatctga atcacaaata ccctaatgaa
      121 ttccctcact ttgcgcctga caacccacaa aattgtatca ggaatgttaa tttattttta
      181 aaggaaaagt attgtaatgt ttcacctccc ccctttggta cttttggtac tactgaagaa
      241 gaagcactca ataacgaaat taatgagctt gaaattattg ccgggcaggc aatactgcct
      301 gaagagagtt ttctctggtt gaaaactgac gaacttgcga ctttctttac ctggactact
      361 atttatctgt cgaaggaaga ccatacaaaa ttcccgaaaa taaaccataa ttcatcagga
      421 ataaaaaacg gaattgaagt caggaaaata cgaacagtta ccgatgaaac gttaaacact
      481 tacaaaaaaa gtaaccttcc ggataatcca tcatcccata acgaacgaat aaaagtaatc
      541 atttcatatt ttgactcctg ccattcttta attaataaaa atgcaaaaag catatacctc
      601 gaggaactca ggcaggcgtg gcttaaaaag aaaagcgctt gtcaaaagtt taagtggctc
      661 cttcctgacg atgatgaaat gtgtcagtgg ttctggacaa gactgaaaca aaaacaggat
      721 gaaaaacatc cagtacataa cccagtgacc cggtggttca taccatcttc aacgaaagaa
      781 tattatcttg cctctcttgt cgcctttgct ttatggaaag aagcgcctga taccattgaa
      841 ctgttcagaa cacgaataaa tcatgcctgg catcagaaaa aacaaaagga caaatatcgg
      901 agtgatggta aaaaggccat taatatacat ataagaagca atgtgaaaga aatgcttgat
      961 gagctcagaa ctgtttacgg tctgagaaca ggagagtttt ttgaaatgct gattacagag
     1021 aaatatcttg aacacaagcg aaggaagtaa ttcattacct gttacattct aaataatctc
     1081 agacagatat tatctccgtg aatcccccgc cacctttccg gtgcgcgggg ttttgtcttt
     1141 tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg aaaaaccgct
     1201 ctcctccctg ccttactcac tttcccgtca catcctggaa catctgcgca aactcaccag
     1261 tcacgaaccc gtgattggca ttatgggtaa aagcggggcc ggtaaatctt cactctgtaa
     1321 tgcactgttt cagggggagg tcacaccggt cagtgatgtt cacgccggca cccgggaagt
     1381 gcagcgcttc cgtctgagtg gtcacggtca cagcatggtt atcactgacc tgcccggggt
     1441 gggcgagagc cgggacaggg atgcagagta tgaagccctg taccgtgaca ttctgcctga
     1501 actggacctg gtgctgtggc tgattaaagc cgatgaccgg gccctgtctg tggatgagta
     1561 tttctggcga cacatcctgc agtgcggaca tcagcaggtg ctgtttgtgg tgacgcaggc
     1621 tgacaaaacg gaaccctgcc atgaatggga tatggcaggt attcagcctt ctcccgcaca
     1681 ggaacagaac attcgtgaga aaacggaggc ggtattccgt ctgttccggc ccgtacatcc
     1741 ggttgttgcc gtatcggccc gcaccggctg ggaactggat acgctggtca gtgcgctcat
     1801 gacagcgctt cccgaccatg ccgccagccc cctgatgacc cgactgcagg acgagctgcg
     1861 cacggagtct gtccgctctc aggcccgtga acagtttacc ggtgcggtgg accggatatt
     1921 tgacacagcg gagagcgtct gtgttgcctc tgttgtccgt acggccctgc gcgctgttcg
     1981 tgacaccgtg gtctctgttg cccgcgcggt atggaactgg atcttcttct gaacctgttg
     2041 tggatgatgt cctccctgcc tctgagtctg ctcacaaaag cgctgttttc gttactgtct
     2101 ctcttgtccg tgcaatagac tgataataga ataaaacgat cgatacctat tttaccgatc
     2161 gttaatatcg atcgttatgc taataataac tcctgttagc aacgtgcgca gatacacaca
     2221 gacatgagat tcagggaaca acagagccac acgtcagaaa cttccgtcag ctggacctcc
     2281 ggcactgtaa ccctttacct gccggtatcc acatctgtgg ataccggctt ttttattcac
     2341 cctcactctg attaaggaaa tgctgatgaa acgacatctg aatacctgct acaggctggt
     2401 atggaatcac attacgggcg ctttcgtggt tgcctccgaa ctggcccgcg cacagggtaa
     2461 acgtggcggt gtggcggttg cactgtctct tgccgcggtc acgtcactcc cggtgctggc
     2521 tgctgacatc gttgtgcacc cgggtgaaac agtgaatggc ggaacactgg taaaccatga
     2581 caaccagttt gtatccggaa cagctgatgg cgtgactgtc agtaccgggc ttgagctggg
     2641 gccggacagt gacgaaaaca ccggcgggca atggataaaa gcgggtggca caggcagaaa
     2701 caccactgtc accgcaaatg gtcgtcagat tgtgcaggca ggaggaactg ccagtgatac
     2761 ggttattcgt gatggcggag ggcagagcct taacggactg gcggtgaaca ccacgctgga
     2821 taacagaggt gagcagtggg tacacggggg agggaaagct gccggtacaa ttattaacca
     2881 ggatggttac cagaccataa aacatggcgg actggcaacc ggaaccatcg tcaacaccgg
     2941 tgcagaaggt ggtccggagt ctgaaaatgt gtccagcggt cagatggtcg gagggacggc
     3001 tgaatccacc accatcaata aaaatggccg gcaggttatc tggtcttcgg ggatggcacg
     3061 ggacaccctc atttacgccg gtggtgacca gacggtacac ggagaggcac ataacacccg
     3121 actggagggg ggtaaccagt atgtacacaa cggtggcacg gcaacagaga cgctgataaa
     3181 ccgtgatggc tggcaggtga ttaaggaagg aggaactgcc gcgcatacca ccatcaacca
     3241 gaaaggaaag ctgcaggtga atgccggcgg taaagcgtct gatgtcaccc agaacacggg
     3301 cggtgcactg gttaccagta cagctgcaac cgtcaccggc acaaaccgcc tgggagcatt
     3361 ctctgttgtg gagggtaaag ctgataatgt cgtactggaa aatggcggac gcctggatgt
     3421 gctgaccgga cacacagcca ctaatacccg cgtggatgat ggcggaacgc tggatgtccg
     3481 caacggtggc accgccacca ccgtatccat gggaaatggc ggtgtactgc tggccgattc
     3541 cggtgccgct gtcagtggta cccggagcga cggaaaggca ttcagtatcg gaggcggtca
     3601 ggcggatgcc ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc
     3661 cacggatacc acggtaaatg gcggactgtt caccgccagg ggcggcacac tggcgggcac
     3721 caccacgctg aataacggcg ccatacttac cctttccggg aagacggtga acaacgatac
     3781 cctgaccatc cgtgaaggcg atgcactcct gcagggaggc gctctcaccg gtaacggcag
     3841 cgtggaaaaa tcaggaagtg gcacactcac tgtcagcaac accacactca cccagaaagc
     3901 cgtcaacctg aatgaaggca cgctgacgct gaacgacagt accgtcacca cggatgtcat
     3961 tgctcagcgc ggtacagccc tgaagctgac cggcagcact gtgctgaacg gtgccattga
     4021 ccccacgaat gtcactctcg cctctggtgc cacctggaat attcccgata acgccacggt
     4081 gcagtcggtg gtggatgacc tcagccatgc cggacagatt catttcacct ccacccgcac
     4141 agggaagttc gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga atggcaccat
     4201 cagcctgcgt gtacgcccgg atatggcaca gaacaatgct gacagactgg tcattgacgg
     4261 tggcagggca accggaaaaa ccatcctgaa cctggtgaac gccggcaaca gtgcgtcggg
     4321 gctggcgacc agcggtaagg gtattcaggt ggtggaagcc attaacggtg ccaccacgga
     4381 ggaaggggcc tttatccagg ggaataagct gcaggccggt gcctttaact actccctcaa
     4441 ccgggacagt gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg cagaagtccc
     4501 cctgtatgcc tccatgctga cacaggcaat ggactatgac cggattctgg caggctcccg
     4561 cagccatcag accggtgtaa gcggtgaaaa taacagcgtc cgtctcagca ttcagggcgg
     4621 tcatctcggt cacgataaca acggcggtat tgcccgtgga gccacgccgg aaagcagcgg
     4681 cagctatggc ttcgtccgtc tggagggtga cctgctcaga acagaggttg ccggtatgtc
     4741 tgtgaccgcg ggggtatatg gtgctgctgg ccattcttcc gttgatgtta aggatgatga
     4801 cggctcccgt gccggcacgg tccgggatga tgccggcagc ctgggcggat acctgaatct
     4861 gatacacaac gcctccggcc tgtgggctga cattgtggcc cagggaaccc gccacagcat
     4921 gaaagcctca tcggacaata acgacttccg cgtccggggc tggggctggc tgggttcgct
     4981 ggaaaccggt ctgcccttca gtatcactga caatctgatg ctggagccgc aactgcagta
     5041 cacctggcag ggactctccc tggatgacgg ccaggataac gccagttatg tgaagttcgg
     5101 gcatggcagt gcacaacatg tgcgtgccgg cttccgtctg ggcagccacc acgatatgaa
     5161 ctttggtaaa ggcacctcat cccgtgacac cctgcgcggc agtgccaaac acagtgtgcg
     5221 tgaactgccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca gctcccgggg
     5281 ggacatgagc atgggtacag ccgcagccgg cagtaacatg acgttctcac cgtcacagaa
     5341 tggtacgtca ctggacctgc aggccggact ggaagcccgt gtccgggaaa atatcaccct
     5401 gggcgttcag gccagttatg cccacagcat caacggcagc agcgctgaag gttataacag
     5461 tcaggccaca ctgaatgtaa ccttctgata at
//
LOCUS       DBBBSB010000195         5485 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.198, whole genome shotgun sequence.
ACCESSION   DBBBSB010000195 DBBBSB010000000
VERSION     DBBBSB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.198"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1630)
                     /locus_tag="ACIUS0_003976"
     CDS             complement(<1..1630)
                     /locus_tag="ACIUS0_003976"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGB0221503.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACIUS0_003977"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACIUS0_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACIUS0_003978"
     CDS             complement(2541..3146)
                     /locus_tag="ACIUS0_003978"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGB0221504.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACIUS0_003979"
     CDS             complement(3317..>5485)
                     /locus_tag="ACIUS0_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGB0221505.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DBBBSB010000196         5398 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.199, whole genome shotgun sequence.
ACCESSION   DBBBSB010000196 DBBBSB010000000
VERSION     DBBBSB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.199"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..497
                     /locus_tag="ACIUS0_003980"
     CDS             <1..497
                     /locus_tag="ACIUS0_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HGB0221506.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACIUS0_003981"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACIUS0_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACIUS0_003982"
     CDS             2500..3114
                     /locus_tag="ACIUS0_003982"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGB0221507.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACIUS0_003983"
     CDS             3114..3443
                     /locus_tag="ACIUS0_003983"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGB0221508.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACIUS0_003984"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACIUS0_003984"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGB0221509.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACIUS0_003985"
     CDS             4529..4840
                     /locus_tag="ACIUS0_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGB0221510.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACIUS0_003986"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACIUS0_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DBBBSB010000197         5350 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.201, whole genome shotgun sequence.
ACCESSION   DBBBSB010000197 DBBBSB010000000
VERSION     DBBBSB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.201"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(75..557)
                     /locus_tag="ACIUS0_003987"
     CDS             complement(75..557)
                     /locus_tag="ACIUS0_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221511.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="ACIUS0_003988"
     CDS             complement(625..2574)
                     /locus_tag="ACIUS0_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGB0221512.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="ACIUS0_003989"
     CDS             complement(2659..2994)
                     /locus_tag="ACIUS0_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGB0221513.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="ACIUS0_003990"
     CDS             complement(2994..3350)
                     /locus_tag="ACIUS0_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGB0221514.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="ACIUS0_003991"
     CDS             complement(3350..4846)
                     /locus_tag="ACIUS0_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGB0221515.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="ACIUS0_003992"
     CDS             complement(4843..5004)
                     /locus_tag="ACIUS0_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGB0221516.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="ACIUS0_003993"
     CDS             complement(5014..>5350)
                     /locus_tag="ACIUS0_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221517.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1275 a         1561 c         1150 g         1364 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg tatttttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DBBBSB010000198         5340 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.202, whole genome shotgun sequence.
ACCESSION   DBBBSB010000198 DBBBSB010000000
VERSION     DBBBSB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.202"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACIUS0_003994"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACIUS0_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGB0221518.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACIUS0_003995"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACIUS0_003995"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGB0221519.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACIUS0_003996"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACIUS0_003996"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGB0221520.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACIUS0_003997"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACIUS0_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGB0221521.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DBBBSB010000199         5311 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.203, whole genome shotgun sequence.
ACCESSION   DBBBSB010000199 DBBBSB010000000
VERSION     DBBBSB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.203"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACIUS0_003998"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACIUS0_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGB0221522.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACIUS0_003999"
     CDS             2739..4208
                     /locus_tag="ACIUS0_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGB0221523.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>5311
                     /locus_tag="ACIUS0_004000"
     CDS             4208..>5311
                     /locus_tag="ACIUS0_004000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGB0221524.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPE"
BASE COUNT         1551 a         1281 c         1270 g         1209 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggt aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga a
//
LOCUS       DBBBSB010000200         5290 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.204, whole genome shotgun sequence.
ACCESSION   DBBBSB010000200 DBBBSB010000000
VERSION     DBBBSB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.204"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..171
                     /locus_tag="ACIUS0_004001"
     CDS             <1..171
                     /locus_tag="ACIUS0_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221525.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACIUS0_004002"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACIUS0_004002"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGB0221526.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACIUS0_004003"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACIUS0_004003"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGB0221527.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACIUS0_004004"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACIUS0_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGB0221528.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACIUS0_004005"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACIUS0_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGB0221529.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1302 a         1186 c         1393 g         1409 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt
//
LOCUS       DBBBSB010000201         5281 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.205, whole genome shotgun sequence.
ACCESSION   DBBBSB010000201 DBBBSB010000000
VERSION     DBBBSB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.205"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACIUS0_004006"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACIUS0_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGB0221530.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2583
                     /locus_tag="ACIUS0_004007"
                     /pseudo
     CDS             1957..2583
                     /locus_tag="ACIUS0_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            2782..3870
                     /gene="serC"
                     /locus_tag="ACIUS0_004008"
     CDS             2782..3870
                     /gene="serC"
                     /locus_tag="ACIUS0_004008"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGB0221531.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3941..5224
                     /gene="aroA"
                     /locus_tag="ACIUS0_004009"
     CDS             3941..5224
                     /gene="aroA"
                     /locus_tag="ACIUS0_004009"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGB0221532.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1313 a         1263 c         1358 g         1347 t
ORIGIN      
        1 taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca ggccgtaaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca gtaataacaa
      301 cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg tcgccagacc
      481 caacagctca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt ccgtggtgcc
      781 ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt gcagcagctc
      961 ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag aggcaaagca
     1021 ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc cgccaaggga
     1081 accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg cttcaaccac
     1141 cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga tattcatcgg
     1321 aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt cgctcagtgg
     1501 gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt cgcccagcca
     1561 gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga gtgcagaagc
     1621 cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa ctgttgacta
     1921 tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga tctccaccgt
     1981 atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg cgacagatgt
     2101 cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat gtggcgtttt
     2161 atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg ttgttatacc
     2221 gcctggtaat gtgatggcga gctggcctgg tcgaaactca ataactccaa catgacggaa
     2281 tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca ggtacgtctg
     2341 gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga caaggtgaaa
     2401 ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt gccggagtcg
     2461 gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg ggaaaaacaa
     2521 ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa acgggtgacc
     2581 tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt gtgatgcaag
     2641 ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa tgataaaacc
     2701 gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg ggttaatagc
     2761 aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg tccggcaatg
     2821 ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa cggtcttggt
     2881 acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc agaggaagcc
     2941 gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt attctgccat
     3001 ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga taaaaccacc
     3061 gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc gaaaaaatac
     3121 tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc ggttaagcca
     3181 atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc gaatgaaacc
     3241 atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt ggtcgccgcc
     3301 gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg tgtaatttac
     3361 gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt tcgtgaagat
     3421 ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat cctcaacgat
     3481 aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg tctggtcttt
     3541 aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca gcaaaaagca
     3601 gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt ggcgaaagct
     3661 aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga caaattgttc
     3721 cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt ggtcggcgga
     3781 atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct gacagacttc
     3841 atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc cccacagcca
     3901 gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc tgacgttaca
     3961 acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg tttctaaccg
     4021 cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc tgctggatag
     4081 cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct atacgctttc
     4141 agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg cagaaggtgc
     4201 cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg cagctctttg
     4261 tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc gcccgattgg
     4321 tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg aacaagaaaa
     4381 ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg ttgatggctc
     4441 cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc cggaagatac
     4501 ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca cactcaatct
     4561 gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg tcgtaaaagg
     4621 cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat cttcggcttc
     4681 ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg gtattggacg
     4741 taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg gcgcgaccat
     4801 ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta ttgatatgga
     4861 tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat ttgcaaaagg
     4921 caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc gcctgtttgc
     4981 gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg attacattcg
     5041 tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg atcaccggat
     5101 ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc ttgatcccaa
     5161 atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta gccaggcagc
     5221 ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt cggcacattg
     5281 t
//
LOCUS       DBBBSB010000202         5213 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.206, whole genome shotgun sequence.
ACCESSION   DBBBSB010000202 DBBBSB010000000
VERSION     DBBBSB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.206"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACIUS0_004010"
     CDS             complement(66..827)
                     /gene="kduD"
                     /locus_tag="ACIUS0_004010"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGB0221533.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACIUS0_004011"
     CDS             complement(857..1693)
                     /gene="kduI"
                     /locus_tag="ACIUS0_004011"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGB0221534.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(1980..3161)
                     /locus_tag="ACIUS0_004012"
     CDS             complement(1980..3161)
                     /locus_tag="ACIUS0_004012"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGB0221535.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3416..4645
                     /locus_tag="ACIUS0_004013"
                     /pseudo
     CDS             3416..4645
                     /locus_tag="ACIUS0_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5072..>5213
                     /locus_tag="ACIUS0_004014"
     CDS             5072..>5213
                     /locus_tag="ACIUS0_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0221536.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NSC"
BASE COUNT         1405 a         1291 c         1110 g         1407 t
ORIGIN      
        1 tatgatgatc tagagatata aaaaaccctg ccatgcggca gggtcataaa agtaagagga
       61 atgaattaac gcgccagcca accgccatcc acagcaatgg tataaccatt tacataatct
      121 gaagcgctgg aggcaagaaa cactaccggc cccatcaggt cactcggcag tccccaacgg
      181 ccagctggaa tgcgatcgag aatttccgcg ctacgttgtt catctgcccg cagttgttgg
      241 gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc
      301 cattcgtttg ccatcaatcg cgtcacaccc atcacgccgc tttttgatgc ggtataagaa
      361 ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat cttgccgcca
      421 ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata
      481 ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcgtc ttcgcggcga
      541 atcaatccgg cgttattcac caggatatca atatgaccaa attcagctac cgcgcgatcc
      601 agcagtccag gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc
      661 cccagcgccg tgacctgctc gatggtttca gtcggttcaa cgatgttaat gccaacaatg
      721 tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagcccagt atcacaacca
      781 gtgacgaccg caactttacc ttggagagaa aatgcactta aaatcataac aacaccttat
      841 tcgtttatgc ccacaactag cgcaaatctt taacgcccac atggtccata tcattaaaga
      901 cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg acgccggaat
      961 ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
     1021 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
     1081 aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag ttacctggag
     1141 ccagctccgt cagccccata ctcaattgac aggtttccag tacatccggg acaaaatatt
     1201 tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
     1261 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1321 tcgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
     1381 acagggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc gtaccggcac
     1441 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     1501 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
     1561 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
     1621 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcaccg tggatgctct
     1681 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     1741 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
     1801 aaaaataaaa caatgtttta tttttaattt aaggattaaa aaaagtgcag tgtcgatcac
     1861 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
     1921 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     1981 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
     2041 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2101 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
     2161 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2221 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     2281 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     2341 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
     2401 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
     2461 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
     2521 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
     2581 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2641 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2701 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
     2761 agctgttcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2821 ccacctgcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     2881 gctttaagac cggaaccgca aacgtcatta atcgtgattg cagaaacgct gttaggaaga
     2941 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     3001 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     3061 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
     3121 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     3181 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
     3241 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
     3301 gcaaccacta acccaccaat agatgggtag atagggcgtt aatctcccat acttaacttg
     3361 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     3421 taatatttgg tctaaagaag aaactctgtg aagtttcgcg ctctatggca cagccgttga
     3481 tgcaggcacg ctattccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
     3541 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
     3601 cctctcatca aaaacatcag caggtgaagg gataacgggc gcggtaacac actactatgg
     3661 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3721 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
     3781 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
     3841 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
     3901 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacaac
     3961 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4021 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4081 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4141 cataagtgta ctgttctttg tctttagctg cctgctttct attccatctt cgtatattga
     4201 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4261 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4321 gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa cactacagca
     4381 ggttggtgta aagaaaagtc gtgcatttaa ccacgcacta tcaattatgt tggtatcgct
     4441 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
     4501 cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt atctcatccc
     4561 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     4621 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
     4681 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
     4741 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
     4801 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
     4861 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
     4921 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atatcttaat
     4981 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaagg
     5041 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
     5101 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
     5161 ccaaataatg ccagaagagt caccgcgttt gcagtactgc gcaaactcgt gtt
//
LOCUS       DBBBSB010000203         5183 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.207, whole genome shotgun sequence.
ACCESSION   DBBBSB010000203 DBBBSB010000000
VERSION     DBBBSB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.207"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..746
                     /locus_tag="ACIUS0_004015"
     CDS             <1..746
                     /locus_tag="ACIUS0_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0221537.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACIUS0_004016"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACIUS0_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGB0221538.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="ACIUS0_004017"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="ACIUS0_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGB0221539.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="ACIUS0_004018"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="ACIUS0_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGB0221540.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5183
                     /locus_tag="ACIUS0_004019"
     CDS             5041..>5183
                     /locus_tag="ACIUS0_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGB0221541.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1481 a         1103 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tac
//
LOCUS       DBBBSB010000204         5170 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.208, whole genome shotgun sequence.
ACCESSION   DBBBSB010000204 DBBBSB010000000
VERSION     DBBBSB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.208"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..265
                     /locus_tag="ACIUS0_004020"
     CDS             <1..265
                     /locus_tag="ACIUS0_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGB0221542.1"
                     /translation="NLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLD
                     NMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            326..3805
                     /locus_tag="ACIUS0_004021"
     CDS             326..3805
                     /locus_tag="ACIUS0_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221543.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            3873..4472
                     /locus_tag="ACIUS0_004022"
     CDS             3873..4472
                     /locus_tag="ACIUS0_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGB0221544.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4536..>5170
                     /locus_tag="ACIUS0_004023"
     CDS             4536..>5170
                     /locus_tag="ACIUS0_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGB0221545.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         1214 a         1261 c         1620 g         1075 t
ORIGIN      
        1 taatctgctg ttttctctcg gtgccagtat gacgcttggc ggtgttgcac agatgctggc
       61 accgaaagcc agaactcccc gtacacagac aacggataac ggcaaacaga acacctgttt
      121 ctcctcactg gataacatgg ttgcccaggg caatgttctg cctgttctgt acggtgaaat
      181 gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg gcagacgaag gggatggtgg
      241 tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat gtgaaaccgc ctgcgggcgg
      301 ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc agtaaggggc ataccccgcg
      361 cgaagcgaag gacaacctga aatccacgca gttgctgagt gtgattgatg ccatcagcga
      421 agggccggtt gacggtccgg tggatggatt aaaaagcgtg ctgctgaacg gtacgccggt
      481 cctggatagc gaggggaaga caaacttttc cggtgttacg gtggtgttcc gcgccggcga
      541 gcaggagcag acaccgccgg aggggtttga atcttccggc tcagagactg tgctgggtac
      601 ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg tcggcaaaca ttgatcgtct
      661 gcgcctgacc ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg accggaatcc
      721 gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt ggctgggtga cggaaaaaga
      781 catcaccatt aagggcaaaa ccacttcgca gtatctggcc tcggtggtgg tgggtaacct
      841 gccgccgcgc ccgtttaata tccggatgcg caggatgaca ccggacagca ccacagacca
      901 gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc atcgatgtga aacagtgcta
      961 cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag cagttcggta accagcaggt
     1021 gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg ccgtcgaact ataacccgca
     1081 gacgcggcaa tacagcggta tctgggacgg aacgcttaag ccagcataca gcaacaacat
     1141 ggcctggtgt ctgtgggaca tgctcactca tccgcgctac ggcatgggga aacgtcttgg
     1201 tgcggcagat gtggacaaat gggcgctgta tgtcatcggc cagtactgcg accagtcagt
     1261 gccggacgga tttggcggca cggagccgcg catcacctgt aacgcttacc tgaccacaca
     1321 gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg
     1381 gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg gataaagtgt ggacctataa
     1441 ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc cgctacagct tcagcgccct
     1501 gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat ccggacaacg gtcatgagac
     1561 ggcgacagag cttgtggagg acacgcaggc cattgtccgt tacggtcgta acgtcacgaa
     1621 gatggatgcc tttggctgta ccagccgggg acaggcgcac cgcgccgggc tgtggctgat
     1681 taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc gtcggcgcag aagggcttcg
     1741 ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat tatgccggta tcagcaccgg
     1801 tggtcgcgta ctggcggtga acagccagac ccggacgctg acgctcgacc gtgaaatcac
     1861 gctgccgtcc tccggtacca cgctgataag cctggttgac ggaaatggca atccggtcag
     1921 cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa gtgagccggg ttcctgacgg
     1981 cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg ctgcgccagc gcctgttccg
     2041 ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt
     2101 gccggaaaaa gaggccatcg tggataacgg ggcacacttt gacggtgacc ggcgcggcac
     2161 ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc gccgaagtca ccgcagacag
     2221 cggggaatat caggtgctgg cgcgatggga cacgccgaag gtggtgaagg gtgtgagctt
     2281 cctgcttcgc ctgaccgtgg cagcggacga cggcagtgag cggctggtca gcacagccag
     2341 gacgacggaa accacatacc gcttcaggca gctggcgctg gggaattaca gtctgacagt
     2401 ccgggcggta aatgcccggg ggcagcaggg cgatccggcg tcggtatcgt tccggattgc
     2461 ggcaccggca gcgcctgtca ctattgaact gataccgggg tattttcaga taacggtggt
     2521 cccgaaactg gctgtatatg acccgacggt gcagtttgag ttctggtttt cggaaaagcg
     2581 gattgcggat atcaggcagg ttgaaaccag cgcccgctat cttggcacgg cgctgtactg
     2641 gatagccgcc agtatcaata tcaggccggg ccatgattat tatttttacg ttcgcagtgt
     2701 gaacaccgtt ggcaaatcgg catttgtgga ggccgtcggt cgggcgagcg atgatgcgga
     2761 aggttatctg agtttttata aagggttgat caataaaacg catctcggca aggagttgtg
     2821 gacgcagatt gataacggtc agcttgcgcc ggacctgact gaaatcagga cgtccattac
     2881 gaatgtcagc aatgaaatca cgcaaaccgt caataaaaaa ctggaaaatc agagtgctgc
     2941 aatccagcag atacagaaag ttcaggttga tacaaataat aacctgaaca gcatgtgggc
     3001 tgtgaagctg caacagatga aggacggacg cctttatatt gcgggtatcg gtgccggtat
     3061 tgagaatacg ccagcaggaa tgcagagtca ggtgctgctg gcggcagaca ggattgcgat
     3121 gattaatcct gcgaatggca acacaaagcc gatgtttgtt ggtcagggcg atcagatatt
     3181 catgaacgac gtgttcctga aacgcctgac ggctccgacc attaccagcg gcggtaatcc
     3241 tccggcattt tccctgacac ctggcggacg gctgacggcg aaaaatgccg atatcagcgg
     3301 taacgtgaac gcgaactccg ggacgctcaa caacgtcacg attaataaga actgtcgggt
     3361 tctgggaaaa ttgtccgcga accagattga aggcgatctc gttaaaacag tgggcaaacc
     3421 tttcccacgg gactcccggg caccggagag gtggccatca gggaccatta ccgtcagggt
     3481 ttatgacgat cagccgtttg atcggcaaat tgttattccc gcggtggcgt ttcgcggtgc
     3541 taaacatgag cggaagaata acaatattta ttcgtcatgc cgcctgatag tgaagaaaaa
     3601 cggtgctgaa atttataacc gaacgaccct ggataatacg ctgatatata cgggtgttat
     3661 tgatatgcct gccggtcacg gtcacatgac gctggagttt tctgtatcgg catggctggt
     3721 aaatggctgg tatcccacag caagtatcag cgatttgctg gttgttgtga tgaagaaatc
     3781 cacagcaggt atcagtatca gctgaatttt ataacccaga tacgggcacc agaaatggtg
     3841 ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa actttatgct gccattttgt
     3901 ccgcagccat ctgtctggct gtatccggtg cgcctgcatg ggcgtctgaa catcggtcca
     3961 cgctgagcgc ggggtatctt catgcctcga cgaacgctcc cggcagtgat gatctgaacg
     4021 ggattaacgt gaaataccgt tatgagttta cggacacgct ggggctggtg acgtcattca
     4081 gctatgccaa cgctgaagat gagcaaaaaa cgcattacag tgatacccgc tggcatgagg
     4141 attccgtgcg taaccgctgg ttcagcgtga tggcggggct gtctgtgcgc gtgaatgaat
     4201 ggttcagcgc gtatgcgatg gcgggcgtgg cttacagccg tgtgtcgacc ttctccgggg
     4261 attatctccg cgtaactgac aacaagggga aaacgcacga tgtgctgacc gggagtgatg
     4321 acaaccgcca cagcaacacg tctctggcgt ggggggctgg cgtgcagttt aacccgaccg
     4381 aatccgtggc cattgacctt gcttatgaag gttccggcag tggcgactgg cgcactgacg
     4441 gttttatcgt gggtgtcggt tataagttct gattagccag gtaacacagt gttatgacag
     4501 cccgctggtt caggcgggct ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac
     4561 tgaaagatgg ggcgggtaaa cctgtcgtaa attgtgcgat tgaactgcgg gccagaagaa
     4621 ccagtccgac cgttgtggca cacgttgttg ccacttgcgt gacggacaat aacggtgctt
     4681 atgtgattga ggctgagccg gggtattacg aggttgcgct tcactgtaac ggctggcagc
     4741 caacccgtgt cggggatatt gatgtggcac cgactgatgc accggggaca ctgaacgcgt
     4801 ttctgaatgc accaaaggat ggtgatttac gtccggaggt gatgaagcgc tttgaggaaa
     4861 tggtggcgca ggcgcagcag agtgccgggg ctgcagccgg aaacgcacag cagacggcgc
     4921 aggatgtggc ggcagccgca acggcccgtg atgatgcaca acgttttgcg gagaaagccc
     4981 gacaggatgc aaccgtcaca gctgaggaca gaaaggccac tgcggaagat gtgacaagca
     5041 caggagcaaa tgcagccgca gccggacaga gcgcacagga tgccgcaggt tatgcccgcg
     5101 cagcagaaca ggccaaaaat gacattgatg ctgcgctgac cggcactctg aaaatggcta
     5161 accatctgtc
//
LOCUS       DBBBSB010000205         5164 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.209, whole genome shotgun sequence.
ACCESSION   DBBBSB010000205 DBBBSB010000000
VERSION     DBBBSB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.209"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..221
                     /locus_tag="ACIUS0_004024"
     CDS             <1..221
                     /locus_tag="ACIUS0_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221546.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="ACIUS0_004025"
     CDS             complement(393..722)
                     /locus_tag="ACIUS0_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGB0221547.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="ACIUS0_004026"
     CDS             complement(727..912)
                     /locus_tag="ACIUS0_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGB0221548.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="ACIUS0_004027"
     CDS             complement(909..3548)
                     /locus_tag="ACIUS0_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGB0221549.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="ACIUS0_004028"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="ACIUS0_004028"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGB0221550.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5164
                     /gene="hslJ"
                     /locus_tag="ACIUS0_004029"
     CDS             4856..>5164
                     /gene="hslJ"
                     /locus_tag="ACIUS0_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGB0221551.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1308 a         1323 c         1203 g         1330 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattagcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaaccagcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcaaaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taac
//
LOCUS       DBBBSB010000206         5151 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.210, whole genome shotgun sequence.
ACCESSION   DBBBSB010000206 DBBBSB010000000
VERSION     DBBBSB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.210"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            190..447
                     /locus_tag="ACIUS0_004030"
                     /pseudo
     CDS             190..447
                     /locus_tag="ACIUS0_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACIUS0_004031"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACIUS0_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGB0221552.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACIUS0_004032"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACIUS0_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGB0221553.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACIUS0_004033"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACIUS0_004033"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGB0221554.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="ACIUS0_004034"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="ACIUS0_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3215..3431
                     /locus_tag="ACIUS0_004035"
                     /pseudo
     CDS             3215..3431
                     /locus_tag="ACIUS0_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3605..4420)
                     /locus_tag="ACIUS0_004036"
     CDS             complement(3605..4420)
                     /locus_tag="ACIUS0_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0221555.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5151
                     /locus_tag="ACIUS0_004037"
     CDS             4517..>5151
                     /locus_tag="ACIUS0_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGB0221556.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1176 c         1226 g         1375 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaaca
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DBBBSB010000207         5128 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.211, whole genome shotgun sequence.
ACCESSION   DBBBSB010000207 DBBBSB010000000
VERSION     DBBBSB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.211"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..244
                     /locus_tag="ACIUS0_004038"
     CDS             <1..244
                     /locus_tag="ACIUS0_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221557.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACIUS0_004039"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACIUS0_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACIUS0_004040"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACIUS0_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGB0221558.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACIUS0_004041"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACIUS0_004041"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGB0221559.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACIUS0_004042"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACIUS0_004042"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGB0221560.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACIUS0_004043"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACIUS0_004043"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGB0221561.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1174 a         1301 c         1466 g         1187 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaata
//
LOCUS       DBBBSB010000208         5101 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.212, whole genome shotgun sequence.
ACCESSION   DBBBSB010000208 DBBBSB010000000
VERSION     DBBBSB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.212"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(12..323)
                     /locus_tag="ACIUS0_004044"
     CDS             complement(12..323)
                     /locus_tag="ACIUS0_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGB0221562.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="ACIUS0_004045"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACIUS0_004045"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="ACIUS0_004046"
     CDS             702..1055
                     /locus_tag="ACIUS0_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGB0221563.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="ACIUS0_004047"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="ACIUS0_004047"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGB0221564.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="ACIUS0_004048"
     CDS             complement(2811..3281)
                     /locus_tag="ACIUS0_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGB0221565.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="ACIUS0_004049"
                     /pseudo
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="ACIUS0_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4120..4338)
                     /locus_tag="ACIUS0_004050"
     CDS             complement(4120..4338)
                     /locus_tag="ACIUS0_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGB0221566.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="ACIUS0_004051"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="ACIUS0_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGB0221567.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1377 a         1177 c         1221 g         1326 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtgc
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgaactatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttactg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 t
//
LOCUS       DBBBSB010000209         5023 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.213, whole genome shotgun sequence.
ACCESSION   DBBBSB010000209 DBBBSB010000000
VERSION     DBBBSB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.213"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACIUS0_004052"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACIUS0_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGB0221568.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACIUS0_004053"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACIUS0_004053"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGB0221569.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="ACIUS0_004054"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="ACIUS0_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGB0221570.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="ACIUS0_004055"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="ACIUS0_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGB0221571.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5023
                     /gene="yidZ"
                     /locus_tag="ACIUS0_004056"
     CDS             4378..>5023
                     /gene="yidZ"
                     /locus_tag="ACIUS0_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGB0221572.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1255 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cct
//
LOCUS       DBBBSB010000210         4999 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.214, whole genome shotgun sequence.
ACCESSION   DBBBSB010000210 DBBBSB010000000
VERSION     DBBBSB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.214"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(212..796)
                     /gene="kptA"
                     /locus_tag="ACIUS0_004057"
     CDS             complement(212..796)
                     /gene="kptA"
                     /locus_tag="ACIUS0_004057"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGB0221573.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1046..1729
                     /locus_tag="ACIUS0_004058"
     CDS             1046..1729
                     /locus_tag="ACIUS0_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGB0221574.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1726..2187
                     /locus_tag="ACIUS0_004059"
     CDS             1726..2187
                     /locus_tag="ACIUS0_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGB0221575.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2200..3372
                     /gene="iadA"
                     /locus_tag="ACIUS0_004060"
     CDS             2200..3372
                     /gene="iadA"
                     /locus_tag="ACIUS0_004060"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGB0221576.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3437..4348
                     /gene="hypT"
                     /locus_tag="ACIUS0_004061"
                     /gene_synonym="qseD"
     CDS             3437..4348
                     /gene="hypT"
                     /locus_tag="ACIUS0_004061"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGB0221577.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4341..4733)
                     /gene="iraD"
                     /locus_tag="ACIUS0_004062"
     CDS             complement(4341..4733)
                     /gene="iraD"
                     /locus_tag="ACIUS0_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGB0221578.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4730..4813)
                     /gene="idlP"
                     /locus_tag="ACIUS0_004063"
     CDS             complement(4730..4813)
                     /gene="idlP"
                     /locus_tag="ACIUS0_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGB0221579.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4929..>4999)
                     /locus_tag="ACIUS0_004064"
     CDS             complement(4929..>4999)
                     /locus_tag="ACIUS0_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221580.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1185 a         1310 c         1246 g         1258 t
ORIGIN      
        1 tctggaaata taggggcaaa tccacggtat gaccggaagc gacgctgatt ccagtcccca
       61 actgaccgcc tgccgtaaat cactttaatt tcagcagttg ttattaaagg ataccctttt
      121 acgagggtat cccttcagac tgataacaaa cgctacattg ccggatgtgc tgcgcttacc
      181 aggcctatat aacccctgca atatattgaa tttattgttt ttgtacccca gtcggcataa
      241 acgaccactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg ttttccgctt
      301 gccagaaagg gatgcctcgt ttcgccattt cctgtgcttt gacggttaag acaaccggcg
      361 atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga tgcacgtaat
      421 gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac
      481 cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg
      541 aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa cgctttttat
      601 cgctggttgc gaccacggta tccagcaatg cacgggttaa tcgcttgccc gctttttggg
      661 cacaaagtat gagcttattg atatccgccc atccttcacg gtccagtacg ataccaatcg
      721 cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc agctcttttt
      781 cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt actaatgtca
      841 ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gcagaacctt ttccacacag
      901 cgtaattttt cccctatgca aaaaacgcat agccagaaac atacttgcat tttccccata
      961 cccacatggc tgataaggtt tttcagttcc aggaattttc ctggctcaag caacacacaa
     1021 acacaaaaaa acaatgggga ttgttatgac acaacaaggg gatgctgtag ccggggagct
     1081 ggcgacagaa aaagtgggca ttaaagggta tctggctttt tttctcacca taatcttttt
     1141 tcccggtgtt ttttccggaa cagacagctg gtggcgcgtt tttgattttt ccgttctgaa
     1201 cggatcgttt gggcaactgc ctggtgcgaa tggagccacc acctcatttc gcggggtggg
     1261 tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt cagtcattct
     1321 ctctctgggg attatttcaa ttaccgatgg tcttggcgga ttacgcgccg cgcagcagtt
     1381 aatgacacct gtgctaaaac cgttgttggg cattccaggg atctgctcgc tggcactgat
     1441 cgctaacctg caaaacacgg atgccgccgc cggtatgacc aaagagctgg cgcaggaagg
     1501 tgaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg gtagcgccat
     1561 catcaccaac tacttctctt ccggcgtcgc ggtgtttgcc tttctgggca cgtccgtaat
     1621 cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt tgcgcgtctg
     1681 gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac aactcaggtc
     1741 cgcaaaaacg tcatggatat gtttatcgat ggcgcgcgcc gtggctttac tatcgccacg
     1801 accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct gaaaattacc
     1861 ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg gggattaccc
     1921 ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc agtcggtgtt
     1981 gccgccagtc tggcaaccgc tgacgcatta accggccacg atgtcaccgt tttactgccc
     2041 gcaatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg gacagccgaa
     2101 gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc attgctgtcg
     2161 atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta taccgcagcc
     2221 ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg aatttgcgat
     2281 gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca atatcccttc tgacattgta
     2341 ccgaactgca cggttgtcga tctcagcggg cagatcctct gcccaggttt tattgatcaa
     2401 cacgtccatt tgattggcgg tggcggcgaa gcaggtccca cgacgcgcac gccggaagtg
     2461 gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct gggcaccgac
     2521 tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa tgaagaaggc
     2581 atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcactat tacgggttcc
     2641 gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgcgc catctctgat
     2701 caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcaga atcccgcgtt
     2761 ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga cagtaaaaag
     2821 gcgttaaagc ctatttatga cctgctggaa aactgcgatg tgccgatcag caagctgctg
     2881 ccgacccacg ttaaccgcaa cgtaccgttg tttgagcagg cgctggagtt cgcgcgcaaa
     2941 ggcggcacca tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc cgaaggtatt
     3001 gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc cgacggcaac
     3061 ggtagccagc cgttcttcga tgacgaaggg aatttaaccc atatcggtgt tgcaggtttt
     3121 gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagact atgatttcag tatcagcgat
     3181 gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acatgagcgg gaaaggcgaa
     3241 attctgccag gcaatgacgc agacttactg gtcatgacgc cagaattgcg cattgagcag
     3301 gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa aggaacgttt
     3361 gaaacggctt aaacggttgt cagccgccct aatggacgta gaatgccccc aagggcggct
     3421 gacagagtaa aacgtaatgg atgactgtgg tgcggttttg cataatattg aaaccaaatg
     3481 gctttatgat tttctgactc tggaaaaatg ccgcaatttt tcccaggcgg cagtcagtcg
     3541 caacgtctcg caaccggcat tcagccgccg catccgtgca ctggaacagg cgattggcgt
     3601 tgaattgttt aaccgccagg tgacgccact gcaactctca gaacaaggga aaatcttcca
     3661 ttcgcagatc cgccatctgt tgcaacagtt agaaagcaac cttgcagagc tgcgtggcgg
     3721 cagcgattac gcgcaacgta aaatcaagat agccgctgca cactctcttt ccctcggtct
     3781 gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg aagccattga
     3841 tgtcgatgaa gcggtcgata aactgcgtga agggcaaagt gactgtattt tttcctttca
     3901 cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat ctcaattgtt
     3961 ccctgtctgc gccagtgacg aacacggaga agcacttttt aacctcgcgc agccacactt
     4021 tccgttactg aattacagcc gcaactccta catggtgcga ttgattaatc gcaccctgac
     4081 gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg agcttttaaa
     4141 gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtatgcca tccaacaaga
     4201 aattcgcagc gggcagctcg ttgtgctgaa tcgggacgaa caggtgatcc cgattcaggc
     4261 ctacgcatac cggatgaata cccgcatgaa tccagttgcc gaacgcttct ggcgtgaact
     4321 gcgcgagctg gagattgtgc ttagctgaca ttctcaagcg tcgcactgcg ccaaccgcca
     4381 ttatcccaga ccagatcaaa cgttagcggt tcatcgtcgt ggtacagcat tatctcaagg
     4441 ctgatcgcca gagtgtggca gtacgcttct ttcaaattaa cctgcaacgc cgcaatccgt
     4501 ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg gagatgtttg
     4561 tcgctcgggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga cggtaatgcc
     4621 gaatgaggag aattaaaaag agtgaggaga tcgctgcata ccgatttacg cagcaatgac
     4681 gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat cattttgcgc
     4741 actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca ctgtattgat
     4801 gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct gagacaggcc
     4861 tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact gacttttcag
     4921 gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaacgct agtatattct
     4981 gaatattccg aatagttac
//
LOCUS       DBBBSB010000211         4888 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.215, whole genome shotgun sequence.
ACCESSION   DBBBSB010000211 DBBBSB010000000
VERSION     DBBBSB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.215"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            17..544
                     /locus_tag="ACIUS0_004065"
     CDS             17..544
                     /locus_tag="ACIUS0_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGB0221581.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            602..835
                     /locus_tag="ACIUS0_004066"
     CDS             602..835
                     /locus_tag="ACIUS0_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGB0221582.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            896..2919
                     /locus_tag="ACIUS0_004067"
                     /pseudo
     CDS             896..2919
                     /locus_tag="ACIUS0_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            2988..3422
                     /gene="psiB"
                     /locus_tag="ACIUS0_004068"
     CDS             2988..3422
                     /gene="psiB"
                     /locus_tag="ACIUS0_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGB0221583.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3419..4138
                     /locus_tag="ACIUS0_004069"
     CDS             3419..4138
                     /locus_tag="ACIUS0_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGB0221584.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4360..4509
                     /gene="mok"
                     /locus_tag="ACIUS0_004070"
                     /pseudo
     CDS             4360..4509
                     /gene="mok"
                     /locus_tag="ACIUS0_004070"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4451..4576
                     /locus_tag="ACIUS0_004071"
     CDS             4451..4576
                     /locus_tag="ACIUS0_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HGB0221585.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT         1099 a         1289 c         1519 g          981 t
ORIGIN      
        1 cagaaggaga cacatcatgg cagttcgtgg cattaacaag gtcattcttg tcggtcgtct
       61 gggaaaagat ccggaagtcc gttacatccc caacgggggc gcagtggcaa acctgcaggt
      121 ggccacgtca gaaacctggc gcgacaaaca gacgggggaa atgaaggagc agacggaatg
      181 gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc tgcgtaaggg
      241 ggcgcaggtc tacattgaag gccagcttcg cacccgtagc tgggaagata acggtattac
      301 ccgttacgtc accgaaatcc ttgttaaaac cacgggcacc atgcagatgc tggggagtgc
      361 accacagcag aacgctcagg tgcaaccgca gcctcagcag aatggtcagt cacagagtgc
      421 tgacgcgacg aaaaaaggga gcgcgaaaac gaaaggccgt ggacgtaagg ctgcgcagcc
      481 ggagcctcag ccgcaaccac cggaggggga ggattacggg ttttcagacg atatcccgtt
      541 ctgactggcc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagatatgag
      601 gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta cccgcaaaca
      661 ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgacc acggcgaaca
      721 gtttcgcctg gttgtccgta ataacggaac aatggtctgg cgtacctgga attttgagga
      781 cggtgccggg tactggatga accatgtcat ccgtgatttc gggatcctta agtaagagag
      841 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
      901 agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacctg caaaaacgca
      961 ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc
     1021 gctgattaag tcaccactga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagcga
     1081 actggctgat tccattaagg gggtcggcct gctgcagaat ctggttgttc atgccctgcc
     1141 aggtgaccgc cacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc
     1201 agagcgtggc atcattccgg ctgactggcc tgtccgcgtg aaaattattt cgcaggaact
     1261 ggcgacggcc gcatcgatga ccgagaacgg tcatcgtcgg gatatgcacc cagccgaaca
     1321 gattgccgga ttctgtgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt
     1381 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggctgacc ttgcgcctgt
     1441 catcctcgat gcgctggcag aagacctcat caccaccgag cactgtcagg cgctggcgct
     1501 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
     1561 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
     1621 agggaacagt aaattccgct tcgtgggggc tgatgccttc tcgccagatg aactgcgcac
     1681 tgacctgttc agcgacgacg agggtggcta tgtggactgc gtggcgctcg atgccgccct
     1741 gctggaaaaa ctccaggctg ttgctgaaca ccttcgggaa gccgaaggct ggggatggtg
     1801 cgccggacgc atggagcctg tcggtgagtg ccgtgaggat gccggaacat accgctgtct
     1861 gccggagccg gaagcggtgc tgacggaggc ggaagaagaa cgtctgaacg aactgatgac
     1921 gcgttacgac gcgctggaaa accagtgtga ggaatctgac ctgctggaag cagaaatgaa
     1981 gctgatgcgc tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagcgg
     2041 tgtggtggtg tcctggcgtt atggcaacgt gtgtgtccag cgtggtgtgc agttgcgcag
     2101 cgaggatgat gcggctgacg acgctgaccg cacggaacag gtgcaggaga aagcgcccgt
     2161 ggaggaaatc agtctgccac tgctgacgaa aatgtcttcc gaacgcacgc tggcagtcca
     2221 ggcagcactg atgcagcaac cggacaaatc tctggcactg ctggcatgga cgctctgccc
     2281 tgatgaatcc cctaatgatt tttaccaaaa tcattagggg attcatcagc gctctgcctg
     2341 aatgtgtttg gcagcggagc gtacagtaaa ccagcacaaa tcagcctgga atgtaaacat
     2401 tattcgctga ccagcgatgc gccatcgggg aaggacggtg ccgcattcct ggcgctgatg
     2461 gcagaaaaag cccgtcttgc agccctgtta ccggagggat ggtcacggga tatgacgaca
     2521 ttcctgtcac tcagccagga ggtactgtta tccctgctca gtttctgcac cgcgtgcagc
     2581 atccacggcg ttcagacccg cgagcatggt cacacgtcac gcagtccgct ggactcgctg
     2641 gagagcgcta tcggattcca catgcgcgac tggtggcagc cgacgaaagc aaacttcttc
     2701 ggacacctgc aaaagccgca gattatcgat gccctgaatg atgccggact gtccggtgct
     2761 gcgcgggacg cggagaaaat gaagaaaggc gatgccgctg aacatgcaga gttccacatg
     2821 aaagacaacc gctgggtgcc tggctggatg tgtacaccac gtccacaggc agagacggaa
     2881 accacggagt accgtgacga ccaggcagaa gccgcctgac cgccagtcat aaaccattta
     2941 tcacgccgcc ctgccggaga cggggcggca gcaagggaga taccgtgatg aaaactgaac
     3001 tgaccctgaa tgtattacag accatgagcg cacaggaata tgaagatatc cgggctgccg
     3061 gaagtgatga acgtcgtgaa ctcacacatg ccgtgatgcg ggagctggat gcgccggata
     3121 actggacgat gaacggcgag tatggcagcg agttcggcgg ctttttcccc gtccaggtcc
     3181 gtttcacgcc tgcccacgaa cgttttcacc tggcgttatg ttcaccgggc gatgtctctc
     3241 aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg gttcaggtac
     3301 agcggcggtt tgcaccggaa gccgtcagcc attcactggc actggcggcg tcactggacg
     3361 cgcaggggta cagcgtcagt gacatcatcc atatcctgat ggcggaagga ggtcaggcat
     3421 gagcgcacgt tcacaggcac tggttcccct cagcgcagag caacaggccg catggcgggc
     3481 ggtggcggag acagaaaaac gccgccacca gggaaacacg ctggcggaat atccgtatgc
     3541 gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg acctgcgatt
     3601 tttcatgccg tccctgaccg cagaagaact gcgcggcaac cgttctcagt ggctgtatgc
     3661 cgtcgatgtg ctgattgaaa ctcagggcga ggtctgcctt cttccccttc ccggcgatgc
     3721 agcagagcgg ctgttcccgt cggtgcggtt ccgtgtcagg gagcgcagtc gccacaaatc
     3781 cgcactggtc atgcaaaagt acagccgcca gcaggcgagg gaggcagagc agaaagcccg
     3841 tgcgtaccag gcgctggtcg ctcaggctga aatcgaactg gcattccatt cgccggaaac
     3901 agtcggaagc tggcatgcac gctggagtga ccgggtggca gagcatgacc tggaaaccct
     3961 gttctggcag tggggtgagc gttttccgtc actggccgga atggaacgct ggcagtggca
     4021 ggatatgccg ttctggcagg ttattgcgga agccagtctt gcagcaagag aggccggtca
     4081 cgctgtccgt gagatggagc ggtggatggt gccgaacaaa ctccgggagg cggcgtgatg
     4141 cggcaacaat cacacagatt acccgtaaac agcctgaatg agcgggttat tttcaggaaa
     4201 agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct tgaggctttc
     4261 tgcctcatga cgtgaaggtg gtttgttacc gtgttgtgtg gcagaaagaa gatagccccg
     4321 tagtaagtta attttcatta accaccacga ggcatcccta tgtctagtcc acatcaggat
     4381 agcctcttac cgcgctttgc gcaaggagaa gaaggccatg aaactaccac gaagttccct
     4441 tgtctggtgt gtgttgatcg tgtgtctcac actgttgata ttcacttatc tgacacgaaa
     4501 atcgctgtgc gagattcgtt acagagacgg acacagggag gtggcggctt tcatggctta
     4561 cgaatccggt aagtagcaac ctggaggcgg gcgcaggccc gccttttcag ggctgatgct
     4621 ggtctgactg cactgaagcg cctttataaa ggggctgctg gttcgccggt ggcccctttc
     4681 tccttgctgg cattgtacgg gcatgaacaa ccagactgcc aggaaggccg tttccctccg
     4741 ggaacgggga tgaacgtgcg cctgcggcgc ccgttctttt cccccgcctt ctctgtttat
     4801 gacttaccag aaatcacatc accttccctt tgattattct ctttttcctc ctgctctgac
     4861 tctgattact gctctgactc tgattact
//
LOCUS       DBBBSB010000212         4868 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.216, whole genome shotgun sequence.
ACCESSION   DBBBSB010000212 DBBBSB010000000
VERSION     DBBBSB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.216"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..525
                     /gene="ydcF"
                     /locus_tag="ACIUS0_004072"
     CDS             <1..525
                     /gene="ydcF"
                     /locus_tag="ACIUS0_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGB0221586.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            722..2161
                     /gene="aldA"
                     /locus_tag="ACIUS0_004073"
     CDS             722..2161
                     /gene="aldA"
                     /locus_tag="ACIUS0_004073"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGB0221587.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2203..3203)
                     /gene="gap"
                     /locus_tag="ACIUS0_004074"
                     /pseudo
     CDS             complement(2203..3203)
                     /gene="gap"
                     /locus_tag="ACIUS0_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3392..3922
                     /gene="cybB"
                     /locus_tag="ACIUS0_004075"
     CDS             3392..3922
                     /gene="cybB"
                     /locus_tag="ACIUS0_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGB0221588.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4167..4340
                     /locus_tag="ACIUS0_004076"
     CDS             4167..4340
                     /locus_tag="ACIUS0_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221589.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4366..4434
                     /gene="yncP"
                     /locus_tag="ACIUS0_004077"
     CDS             4366..4434
                     /gene="yncP"
                     /locus_tag="ACIUS0_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGB0221590.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4412..4561)
                     /gene="hokB"
                     /locus_tag="ACIUS0_004078"
     CDS             complement(4412..4561)
                     /gene="hokB"
                     /locus_tag="ACIUS0_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGB0221591.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4662..4802)
                     /locus_tag="ACIUS0_004079"
                     /pseudo
     CDS             complement(4662..4802)
                     /locus_tag="ACIUS0_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1119 c         1207 g         1295 t
ORIGIN      
        1 accactggca gagcagaagc gaccatcctg gtggatatcg ctcatcagtt ctggcatatt
       61 ccgcatgaaa aaatctggat tgaagaccag tcaacaaact gcggtgaaaa cgcacgcttt
      121 agcatcacgc tattgaatca ggccgtagaa cgagttcata cggctatcgt tgtacaggac
      181 cccaccatgc agcggcgcac gatggcaacg ttccgccgta tgaccgggga caatcccgat
      241 gcaccacgct ggttaagtta tcccggattc gttcctcagt taggaaataa cgcagacagt
      301 gtaatcttta ttaatcagtt acaaggatta tggccggttg agcgttatct ctcactactc
      361 actggcgagc tgccgcgttt acacgatgat agcgatggct acggtccccg cgggcgagat
      421 tttatcgttc acgttgattt tccggcagaa gtcatccatg catggcaaac gctgaaacat
      481 gatgcggtgc tcatcgaggc gatggaaagt cgctcgttac gttaaaaatt gcccgtttgt
      541 gaaccacttg tttgcaaacg ggcatgactc ctgactttta tttctgcctt ttattccttt
      601 tacacttgtt tttatgaagc cctccacaga cttgtccttt cccgatgccg tctctctgat
      661 gattgatgtt aattaacaat atattcaccg aaaacaaaca tataaatcac aagagtcgcc
      721 catgtcagta cccgttcaac atcctatgta tatcgatgga cagtttgtta cctggcgtgg
      781 agacgcatgg attgatgtgg taaaccctgc tacagaggct gtcatttccc gcatacccga
      841 tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca gaacgtgcac aaccagaatg
      901 ggaagcgttg cctgctattg aacgcgccag ttggttgtgc aaaatctccg ccgggatccg
      961 cgaacgcgcc agtgaaatca gtgcgctgat tgttgaagaa gggggcaaga tccagcagct
     1021 agctgaagtc gaagtggctt ttactgccga ctatatcgat tacatggcgg agtgggcacg
     1081 gcgttacgag ggcgagatta ttcaaagcga tcgtccggga gaaaatattc ttctgtttaa
     1141 acgtgcgctt ggtgtgacta ccggcattct gccgtggaac tttccgttct tcctcattgc
     1201 ccgcaaaatg gctcccgctc ttttgaccgg taataccatc gtcattaaac ccagtgaatt
     1261 tacgccaaac aatgcgattg cattcgccaa aatcgtcgat gaaataggcc ttccgcgcgg
     1321 cgtgtttaac cttgtactgg ggcgtggtga aaccgttggg caagaactgg cgggtaaccc
     1381 aaaggtcgca atggtcagta tgacaggcag cgtctctgca ggtgagaaga tcatggcgac
     1441 tgcggcgaaa aacatcacca aagtgtgtct ggaattgggt ggtaaagcac cggctatcgt
     1501 aatggacgat gccgatcttg aactggcagt caaagccatc gttgattcac gcgtcattaa
     1561 tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta cagaaaggca tttatgatca
     1621 gttcgtcaat ctgctgggtg aagcgttgca ggcggttcaa tttggtaacc ccgctgaacg
     1681 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
     1741 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
     1801 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggaaa tgtcgattat
     1861 gcatgaggaa acctttggcc cggtgctgcc agttgtcgca tttgacacgc tggaagatgc
     1921 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
     1981 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
     2041 aaacttcgaa gctatgcaag gtttccacgc cggatggcgt aaatccggta ttggcggcgc
     2101 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtctta
     2161 atgagtgaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     2221 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     2281 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacactga
     2341 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     2401 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     2461 ccgagaatgg acaccagctc agtgaccgaa cctgttttca ccggcacgcg ttgcgcatga
     2521 cctttcagtt tgccgctcag ttccgggatc accagaccaa tggcttttgc cgcccccgta
     2581 gtgtggggaa tgatattttc tgccgctgcg cgtgaagcac gtaaatcttt accacgcggg
     2641 ccatccacca gtgactgggt gccagtatag gcatgaatgg tcgtcatcgt gccgacttct
     2701 atcccgaaac tgtcatgcaa ggctttggcc atcggcgcaa ggcagttagt ggtgcatgac
     2761 gccacggaaa caatggtgtc gttgccatcc agagtgtcgt cattaacgtt ataaacgatg
     2821 gttttcattt caccggcagg agcggaaatc aacaccttct tcgcaccagc atcaagatgc
     2881 gcctgcgatt tatcggcgga ggtataaaag ccagtacatt cgacaatgat ttctgcccct
     2941 ttcgctttcc acggaatatt tttagcctct ttttcggcgt aaaccgcgat acttttccca
     3001 tcaacgataa gtgaatcttc cgtaaaatca acgctccagg ggaacggacc atagtttgaa
     3061 tcatgtttca gcagataggc gagaattttt ggggaagtaa gatcattaat agcgacaacg
     3121 tctatgttgc ttttgacttc aagtaaacga cgcaacacca gtcgaccgat acgaccaaaa
     3181 ccgttaatac caactttact catggttttc tcctgtcagg aacgttcgga tgaaaattga
     3241 tcctttccag gcttagacca ggattgtggg atgggcaatc tccattctta cagtgaaacg
     3301 taacgtactg aaaacgggtg aacaatattt aatgaaattt tgagaaaagc ccgttatgtt
     3361 aacggaaaat tatgttaaag caggaaatgt tatggaaaat aaatactcaa ggttacaaat
     3421 cagcattcac tggctggtct ttttgctggt tatcgcagcg tattgcgcaa tggagtttcg
     3481 tggtttcttc ccacgtagcg atcggccgct catcaacatg attcatgttt cctgtggcat
     3541 ctcaattctc gtgctgatgg tggttcgtct tttgttaaga ctgaaatacc caaccccgcc
     3601 gattatacct aagccgaaac cgatgatgac gggactggcg catttgggac atttggtgat
     3661 ttatctcctg tttattgcgc tgccagtgat tggtttggtg atgatgtata accggggcaa
     3721 cccgtggttt gcgtttggtt tgacgatgcc ttacgcttca gaggccaatt tcgaacgggt
     3781 tgatagctta aagtcgtggc atgagacgct ggcgaatctg ggatattttg tcatcgggtt
     3841 gcacgctgcg gctgcactgg cacaccacta tttctggaag gacaacacac ttctacgcat
     3901 gatgccgcgt aaacgttcct gaaggatatt taaagaaaac gcctgtacta aaaccgaccc
     3961 gtggtacagg cgaagaatac gggtctacat cggaagcgcc tattatattt atttgtatga
     4021 caaataaaac cataatcctt gcccatacgt ccatctggct tatttttaat caattcaccc
     4081 gatctttgat ctcatcaacg gtatcaaaat aaaccagcgt ataacgttca aatcgcacat
     4141 aagattcgat aaaccatgga ggttatatga aaaaactggc acttattttg tttatgggaa
     4201 cgcttgtttc cttttatgcc gatgccgggc gcaaaccctg ttctggttcg aaagggggga
     4261 tctcacactg tacggcaggc ggcaaatttg tctgtaatga tggttctatt agtgcatcga
     4321 aaaaaacatg cactaactga agtgtaaaag gggtgccatg agaaaatgaa tctgctagtc
     4381 agatgcgcgg ggaaaatccc cgcgcttgcc cttacctgga cgtgcaggcc atgagcgcag
     4441 caacctcctt atcaccgtcc cggaaccgca gttcgtagag cgtttgtcgg gtcaggagtg
     4501 tgaatgccag aatcgtaatg cagataatga gcagacacac caccagaggg ttgcgcttca
     4561 tagcctttct ccttgccgga tggcgggtaa gaggctaaca atctgaattg cttggttcat
     4621 tcgttggcct cggtttgtac tgaccccaaa aagttggaca gttaaacacg aggcatatag
     4681 gtctgattcc gatattcatt tggagtcaga ccttttaatt tcaggctaat tcttctgctg
     4741 ttgtagtatt caatatattc cgtaacagca tccttcagtt cgcttatatt actgaactca
     4801 ttagactggc cccctgaatc tccagacaac cagtatcact tatttaagtg atagtcttaa
     4861 tactagtt
//
LOCUS       DBBBSB010000213         4821 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.217, whole genome shotgun sequence.
ACCESSION   DBBBSB010000213 DBBBSB010000000
VERSION     DBBBSB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.217"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(337..582)
                     /locus_tag="ACIUS0_004080"
     CDS             complement(337..582)
                     /locus_tag="ACIUS0_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221592.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="ACIUS0_004081"
     CDS             complement(1359..2201)
                     /locus_tag="ACIUS0_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGB0221593.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="ACIUS0_004082"
     CDS             complement(2286..2483)
                     /locus_tag="ACIUS0_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGB0221594.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="ACIUS0_004083"
     CDS             complement(2503..2991)
                     /locus_tag="ACIUS0_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGB0221595.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="ACIUS0_004084"
     CDS             complement(2988..3365)
                     /locus_tag="ACIUS0_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGB0221596.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbeA"
                     /locus_tag="ACIUS0_004085"
     CDS             complement(3412..3789)
                     /gene="cbeA"
                     /locus_tag="ACIUS0_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGB0221597.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="ACIUS0_004086"
     CDS             complement(3869..4090)
                     /locus_tag="ACIUS0_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGB0221598.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /gene="radC"
                     /locus_tag="ACIUS0_004087"
     CDS             complement(4177..4653)
                     /gene="radC"
                     /locus_tag="ACIUS0_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGB0221599.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4669..>4821)
                     /locus_tag="ACIUS0_004088"
     CDS             complement(4669..>4821)
                     /locus_tag="ACIUS0_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGB0221600.1"
                     /translation="PEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSA
                     IMRIID"
BASE COUNT         1127 a         1162 c         1277 g         1255 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtccg gggcagtttt acctccgatg
     2041 ctttccgtca gcaccgagat ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat
     4681 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     4741 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     4801 agcaatgccg gcagcttccg g
//
LOCUS       DBBBSB010000214         4783 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.218, whole genome shotgun sequence.
ACCESSION   DBBBSB010000214 DBBBSB010000000
VERSION     DBBBSB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.218"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACIUS0_004089"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="ACIUS0_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HGB0221601.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACIUS0_004090"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="ACIUS0_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HGB0221602.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACIUS0_004091"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="ACIUS0_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HGB0221603.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACIUS0_004092"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="ACIUS0_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HGB0221604.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4783)
                     /locus_tag="ACIUS0_004093"
     CDS             complement(4148..>4783)
                     /locus_tag="ACIUS0_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGB0221605.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1268 a         1169 c         1129 g         1217 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttttagataa cgg
//
LOCUS       DBBBSB010000215         4603 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.219, whole genome shotgun sequence.
ACCESSION   DBBBSB010000215 DBBBSB010000000
VERSION     DBBBSB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.219"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACIUS0_004094"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="ACIUS0_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGB0221606.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACIUS0_004095"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="ACIUS0_004095"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGB0221607.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACIUS0_004096"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="ACIUS0_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGB0221608.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACIUS0_004097"
     CDS             complement(3930..>4603)
                     /gene="efeU"
                     /locus_tag="ACIUS0_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGB0221609.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DBBBSB010000216         4532 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.220, whole genome shotgun sequence.
ACCESSION   DBBBSB010000216 DBBBSB010000000
VERSION     DBBBSB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.220"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1224
                     /locus_tag="ACIUS0_004098"
     CDS             <1..1224
                     /locus_tag="ACIUS0_004098"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGB0221610.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACIUS0_004099"
     CDS             1224..1958
                     /locus_tag="ACIUS0_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0221611.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..3186
                     /locus_tag="ACIUS0_004100"
     CDS             2425..3186
                     /locus_tag="ACIUS0_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGB0221612.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3260..4532
                     /locus_tag="ACIUS0_004101"
                     /pseudo
     CDS             3260..4532
                     /locus_tag="ACIUS0_004101"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1243 a          862 c         1117 g         1310 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa
     3181 tcataatgta ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg
     3241 actgtcatta attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc
     3301 atttgattct tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca
     3361 gggagagcaa ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga
     3421 tgttggggag tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg
     3481 tttaaccgtt gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac
     3541 tgacaataaa aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta
     3601 tgattttgat agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa
     3661 aagtagttta gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta
     3721 tcagacaaat attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg
     3781 ttatggcagg ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac
     3841 atggagcaag gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg
     3901 aggcataaat gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt
     3961 gtttgacagc ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc
     4021 ggaggacagc tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat
     4081 tattaaacaa aatgggcaaa ttatttttac ccgttctgtg ccgccagggc cttttgcgct
     4141 ggataattta ccaacgctgg ccgttggcgg ggagcttgat gttacagtga gagagagtaa
     4201 tggcgaggag cagtattttt ccgtgccatt tcaaacgccg gccatcgctc tgcatgaagg
     4261 atatttcaaa tatagtgtga tgggggggaa tataaagaag aaggtataaa aagctcccct
     4321 gtctccgtgg gcgaaatcac ggcgatgtac gggcttcctt atggcgtcac ggtgtatgga
     4381 gggattcaaa acgccactca ttttaatgca atatctacgg gtattggcat ttctctgggg
     4441 ctgttaggga gtttatcgac agatattacg cgttcgattg cgaatttata ttatgggaat
     4501 aaatatcgta ttcgttatag taaatccatt ag
//
LOCUS       DBBBSB010000217         4518 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.221, whole genome shotgun sequence.
ACCESSION   DBBBSB010000217 DBBBSB010000000
VERSION     DBBBSB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.221"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..761)
                     /locus_tag="ACIUS0_004102"
     CDS             complement(<1..761)
                     /locus_tag="ACIUS0_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGB0221613.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHE"
     gene            1179..2177
                     /locus_tag="ACIUS0_004103"
                     /pseudo
     CDS             1179..2177
                     /locus_tag="ACIUS0_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2264..2740)
                     /locus_tag="ACIUS0_004104"
     CDS             complement(2264..2740)
                     /locus_tag="ACIUS0_004104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221614.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(2733..3497)
                     /locus_tag="ACIUS0_004105"
     CDS             complement(2733..3497)
                     /locus_tag="ACIUS0_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0221615.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            3743..4504
                     /locus_tag="ACIUS0_004106"
                     /pseudo
     CDS             3743..4504
                     /locus_tag="ACIUS0_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         1425 a          885 c          792 g         1416 t
ORIGIN      
        1 tcttcgtgtt ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc
       61 cctgaaacga gcaatgaata aagtacagcg cctactcttc catctgtatc gccaaaataa
      121 gccgtgttgg cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa
      181 aacacagatt taacatagct atcaaactta tccttaggaa acgtctcttt agagacatcc
      241 caacgccagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
      301 gaaagcccat taactttata aattatgtct gatttatctc cggagaaatt aataaaataa
      361 taaccatcct tacactcaat cttaactgta atgggagacg ataattttat cacatggtta
      421 ttgtctggta taattgtaaa gataaacgtg ttagtagtat tatcggcaaa actattatat
      481 tcttgaatat ctatagatgg ctcactattg taaaaccata ttttctccat ctgaagacta
      541 tgttcattta ccctccagtg accatgttgc aggaaataga tgatatgcat gccaaaatcg
      601 ttatctattt gcgaatcatt atccagctta tcaatttcaa ctatctttga acgatgtgtt
      661 ttatcaacct tgctatggtg ttgatgatta ttaactttat ctgaatgctt atctttatgg
      721 tttgaaggga tgtacttaaa tatattgtca attgctttca taaataaccc gcgatatttt
      781 tgacctgtta gtaaaaaata aatgatacat gtctgaaaga catatttacg acgatataaa
      841 acaataatac ttatatgttc gctgatttta agcaccgaga ctatccggtc ttttactaaa
      901 taatggaaaa caatccatcc ctggaagaca attgtaaaaa aaaacaacag aataagaatt
      961 aatcttagga taaatattta tttatcatgg ccttttgacc gctaaaaaat ggcactcggc
     1021 acagtaaacc ccgctatagt tatgctatgc atttgtcttt aatgatatct cgtcgttata
     1081 ttaacaggct gaaattatca ttaatgcaca tttcaatatt agcagggata cctgaggagt
     1141 ttaaagtgaa catatatatc gggtagcttt tcaaattaat ccctttgatt ataggcttaa
     1201 tttgcatcgc gttaggtggc tttgtgctgg aaagttcagg gcaacgtcac gggtcatgtg
     1261 ctgatttctc tggcggccat atgcctggca ttattcacta ccgcatttat tatcatttcg
     1321 caactcacgc gcggcgttaa tacgttttac aatacattgt tccccattat tggctatgcg
     1381 gggtcaatta tcaccatgat atggggttgg gcactgttag caggtaatga tgtgatggca
     1441 gacgagtttg ttgccggcca tgttattttt ggcgttggta tgattgccgc atgtgtatcg
     1501 acggtggcag cgtcgtccgg tcactttctg ctcattccca aaaatgcagc ggggagcaag
     1561 agcgacggaa caccggtaca ggcttattct tcattaatcg gtaattgcct cattgccgtt
     1621 cccgttttac tcaccctgct cggtttcatt tggtctatta cgctgttaca tagtgccgac
     1681 ataactccgc attatgtcgc cttgggttaa ccgcaatctg tgcctgtcta attggcctag
     1741 tggccacaat cgtccatcaa acacgtaata cgttctcatc caaagagcac tggctgtggt
     1801 gttattgggt tatttttctc ggctcagtca cggtgctgca ggggatatac gtcttagtca
     1861 gttccgatgc aagcgcccga ctggctcccg gcattattct catttgcctc ggaatgatct
     1921 gttacagcat attctcaaaa gtctggctac tggcactggt ctggagacgt acctgttcgt
     1981 tagccaacag aataccgatg ataccggtat ttacctgtct gttttgcttg tttttgtcat
     2041 cattcctcgc ggaaatcgca caaactgagc tggactactt tattccatct cgggttctgg
     2101 ttggattagg cgcagtatgt tttacattgt tctctattgt ttcaatatta gaggccggtt
     2161 ctgcgaaaaa aaattaaaat ttacataatc ccagcgttat tcatatctta acgctggttt
     2221 tttacgaact tagtgaaata atgtagaata taaaattata attttatatt ggagcacatg
     2281 tgttaatgat caaccccggg atactaaaaa tatagacatt attaaacttg cttagcctcg
     2341 cattgccttt acctgttaaa gatgcatcga gaaaattgag tctgaagttc tcatccttta
     2401 tctgattttc cagtcccgaa actttggcct tagcattacc tgagatccgt ccgttactat
     2461 ctattttgta ggtaaaaaaa tacgaatggg aaaatttcct cggtgtatct tcatcagtta
     2521 agccaacaat cgtaaaacta ccgctaccat ctttttttaa aatgactgaa aggttagcag
     2581 agtagtataa agaatgatct gttttagcat ttcttgttaa cgtcgacgta cattcaagag
     2641 acgttgtgct accatcacga gccttccaga gagcaaaaaa acacacctgt agcaatgtga
     2701 aaacaagtat taatataaaa tacattttct tattagtcat ataatagcac ctctgaacaa
     2761 attgcataag tttcgtcatt agaggatttt atacatcttg ataaataccg ctgcccttcc
     2821 ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg cacacagggt
     2881 aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgatgcc aatctcgtta
     2941 agattctctg aaagatataa atcacaggtg tcaatcttac ctgatttaac caatggataa
     3001 tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat aacaattact
     3061 accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc tactttttca
     3121 acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt tatgttttca
     3181 ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac accaatattt
     3241 ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga cagatcaaat
     3301 gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc accattactt
     3361 tccagtaaca ctgccaaaat acaccccgcc gtagctggaa aatttatctc atcctgggta
     3421 tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt gataatgaaa
     3481 aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt ttagcctatt
     3541 cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa gtcatataaa
     3601 acttatatcc acagaataaa aaatcaaaca tgtcagtttt atcaatataa agtatcgcaa
     3661 ggcgaaaagt gatgcaagaa tactcctaag taaatgaaag cgcaccacat gaaattatca
     3721 acacatgtaa tttcatcatt aggtgctatc gaaaataact caggagctac ttaagatgaa
     3781 taatgtctta ctcatgagac ccgatgactc agaatttatg atacctgatg actggtcagg
     3841 gctaaacgca aaaggcatta catacgaaca agataataga gtgacagagt cttttctgcc
     3901 ttttaaatct ttttattgtt caccggtgtg cacgacagtt gaactcatct cataaaataa
     3961 ttgttttgct atgggtatcc gctaccttct ggaaaagaaa agagacttaa ggctaaatca
     4021 ctttaaagcc ttctctcata aatgcgagac aaaacctgac gtgttaatta tgaccgggtg
     4081 caccgtcgct gatacttata gtatattctc acgcattgtt gaagaaaata taatgaaaac
     4141 gatgacacct atcgtcatca ttactgggca tcactcagaa gatctttgtt ctctggcatc
     4201 gaaactggga ggagttaaaa taatatcacc gacagaaaag attgcaaaaa tagaaaacgc
     4261 tatcaatgat gcttttaaca gcaagcctaa aaaaataaga tttttaacag atatactctc
     4321 tcctcaacaa attgagacca tgaaaatggt tgcgcagggt agatccgtta atgagatttc
     4381 agccgcaatg aatattagca tcaagaccgt gtatgcacat aaagttcgtg ctatcaataa
     4441 gctgggaatt aagaaaaaag tttatgaagc gcttttttat aaagcgctaa ttcagttcaa
     4501 ctgatgtaaa aatcaccc
//
LOCUS       DBBBSB010000218         4502 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.222, whole genome shotgun sequence.
ACCESSION   DBBBSB010000218 DBBBSB010000000
VERSION     DBBBSB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.222"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            217..1074
                     /gene="focA"
                     /locus_tag="ACIUS0_004107"
     CDS             217..1074
                     /gene="focA"
                     /locus_tag="ACIUS0_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGB0221616.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1129..3411
                     /gene="pflB"
                     /locus_tag="ACIUS0_004108"
     CDS             1129..3411
                     /gene="pflB"
                     /locus_tag="ACIUS0_004108"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGB0221617.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3603..4343
                     /gene="pflA"
                     /locus_tag="ACIUS0_004109"
     CDS             3603..4343
                     /gene="pflA"
                     /locus_tag="ACIUS0_004109"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGB0221618.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4425..>4502)
                     /locus_tag="ACIUS0_004110"
     CDS             complement(4425..>4502)
                     /locus_tag="ACIUS0_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221619.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1149 a         1107 c         1090 g         1156 t
ORIGIN      
        1 attaactgtt ttagcggagg atgcggaaaa aattcaactc atttgttaat ttttaaaatt
       61 tatttttatt tggataatca aatagttact ccgtatttgc ataaaaacca tgcgagttac
      121 gggcctataa gccaggcgag atatgatcta tatcaatttc tcatctataa tgctttgtta
      181 gtatctcgtc gccgacttaa taaagagaga gttagtgtga aagctgacaa cccttttgat
      241 cttttacttc ctgctgcaat ggccaaagtg gccgaagagg cgggtgtcta taaagcaacg
      301 aaacatccgc ttaagacttt ctatctggcg attaccgccg gtgttttcat ctcaatcgca
      361 ttcgtcttct atatcacagc aaccactggc acaggcacaa tgcccttcgg catggcaaaa
      421 ctggttggcg gcatttgctt ctctctgggg ctgattcttt gtgttgtctg cggagccgat
      481 ctctttactt ccacagtgtt gattgttgtt gctaaggcga gtgggcgcat cacctggtgt
      541 cagttggcta aaaactggct aaatgtctat tttggcaacc tggtcggcgc actgctgttt
      601 gtacttttaa tgtggctttc cggcgagtat atgaccgcaa atggtcaatg gggactaaac
      661 gtcctacaaa ccgccgacca caaagtgcac catactttta ttgaggccgt ctgtcttggt
      721 atcctggcaa acctgatggt atgtctggca gtatggatga gttattctgg ccgcagcctg
      781 atggacaaag cgttcattat ggtgctgccg gtcgcgatgt ttgttgccag cggttttgag
      841 cacagtatcg caaacatgtt tatgatcccg atgggtattg taatccgcga cttcgcatct
      901 ccggaattct ggaccgctgt cggttctgca ccggaaaatt tttctcacct gaccgtgatg
      961 aacttcatca ctgataacct gatcccggtt acgatcggta atattatcgg cggtggtttg
     1021 ttggttgggt tgacatactg ggtcatttac ctgcgtgaaa acgatcacca ttaatggttg
     1081 tcgaagtacg cagtaaataa aaaatccact taagaaggta ggtgttacat gtccgagctt
     1141 aatgaaaagt tagccacagc ctgggaaggt tttaccaaag gtgactggca gaatgaagta
     1201 aacgtccgtg acttcattca gaaaaactac actccgtacg agggtgacga gtccttcctg
     1261 gctggcgcta ctgaagcgac caccaccctg tgggacaaag taatggaagg tgttaaactg
     1321 gaaaaccgca ctcacgcgcc agttgatttt gacaccgctg ttgcttccac catcacctct
     1381 cacgacgctg gctacatcaa taaagcgttg gaaaaagttg ttggtctgca gactgaagct
     1441 ccgctgaaac gtgctcttat cccgttcggt ggtatcaaaa tgatcgaagg ttcctgcaaa
     1501 gcgtacaacc gcgaactgga cccgatgatc aaaaaaatct tcactgaata ccgtaaaact
     1561 cacaaccagg gcgtgttcga cgtttacact ccggacatcc tgcgttgccg taaatccggt
     1621 gttctgaccg gtctgccaga tgcttatggc cgtggccgta tcatcggtga ctaccgtcgc
     1681 gttgcgctgt acggtatcga ctacctgatg aaagacaaat acgctcagtt cacctctcta
     1741 caggctgatc tggaaaacgg cgtaaacctg gaacagacta tccgtctgcg cgaagaaatc
     1801 gctgaacagc accgcgctct gggtcagatg aaagaaatgg ctgcgaaata cggctacgac
     1861 atctctggtc cggctaccaa cgctcaggaa gctatccagt ggacttactt cggctacctg
     1921 gctgctgtta agtctcagaa cggtgctgca atgtccttcg gtcgtacctc caccttcctg
     1981 gatgtgtaca tcgaacgtga cctgaaagct ggcaagatca ccgaacaaga agcgcaggaa
     2041 atggttgacc acctggtcat gaaactgcgt atggttcgct tcctgcgtac tccggaatac
     2101 gatgaactgt tctctggcga cccaatctgg gcaaccgaat ctatcggtgg tatgggcctc
     2161 gatggtcgta ccctggttac caaaaacagc ttccgtttcc tgaacaccct gtacactatg
     2221 gggccgtctc cggaaccgaa catgaccatt ctgtggtctg aaaaactgcc gctgaacttc
     2281 aagaaattcg ccgctaaagt gtccatcgac acctcttctc tgcagtatga gaacgatgac
     2341 ctgatgcgtc cggacttcaa caacgatgac tacgctatcg cttgctgcgt aagcccgatg
     2401 atcgttggta aacaaatgca gttcttcggt gcgcgtgcaa acctggcgaa aaccatgctg
     2461 tacgcaatca acggcggcgt tgacgaaaaa ctgaaaatgc aggttggtcc gaagtctgaa
     2521 ccgatcaaag gcgatctcct gagctacgat gaagtgatgg agcgcatgga tcacttcatg
     2581 gactggctgg ctaaacagta catcactgca ctgaacatca tccactacat gcacgacaag
     2641 tacagctacg aagcctctct gatggcgctg cacgaccgtg acgttatccg caccatggcg
     2701 tgtggtatcg ctggtctgtc cgttgctgct gactccctgt ctgcaatcaa atatgcgaaa
     2761 gttaaaccga ttcgtgacga agacggtctg gctatcgact tcgaaatcga aggcgaatac
     2821 ccgcagtttg gtaacaatga tccgcgtgta gatgacctgg ctgttgacct ggtagaacgt
     2881 ttcatgaaga aaattcagaa actgcacacc taccgtgacg ctatcccgac tcagtctgtt
     2941 ctgaccatca cttctaacgt tgtgtatggt aagaaaactg gtaacacccc agacggtcgt
     3001 cgtgctggcg cgccgttcgg accgggtgct aacccgatgc acggtcgtga ccagaaaggt
     3061 gctgtagcgt ctctgacttc cgttgctaaa ctgccgtttg cttacgctaa agatggtatc
     3121 tcctacacct tctctatcgt tccgaacgca ctgggtaaag acgacgaagt tcgtaagacc
     3181 aacctggctg gtctgatgga tggttacttc caccacgaag catccatcga aggtggtcag
     3241 cacctgaacg ttaacgtgat gaaccgtgaa atgctgctcg acgcgatgga aaacccggaa
     3301 aaatatccgc agctgaccat ccgtgtatct ggctacgcag tacgtttcaa ctcgctgact
     3361 aaagaacagc agcaggacgt tattactcgt accttcactc aatctatgta attagatttg
     3421 actgaaatcg tacagtaaaa agcgtacaat aaaggctcca cgaaagtggg gcctttttta
     3481 gcacgagagc cttttttgtc agctatctat actttaaggt gactgccaaa acagactcga
     3541 cgtagccttc gagctgcgca ccaacacggc ctcagatggg ccacatctgg agaaacaccg
     3601 caatgtcagt tattggtcgc attcactcct ttgaatcctg tggaaccgta gacggcccag
     3661 gtattcgctt tatcaccttt ttccagggct gcctgatgcg ctgcctgtat tgtcataacc
     3721 gcgacacctg ggacacgcat ggcggtaaag aagttaccgt tgaagatttg atgaaggaag
     3781 tggtgaccta tcgccacttt atgaacgctt ccggcggcgg cgttaccgca tccggcggtg
     3841 aagcaatcct gcaagctgag tttgttcgtg actggttccg cgcctgtaaa aaagaaggca
     3901 ttcatacctg tctggatacc aacggttttg ttcgtcgtta cgatccggtg attgatgaac
     3961 tgctggaagt aaccgacctg gtaatgctcg atctcaaaca gatgaacgac gagatccacc
     4021 aaaatctggt tggagtttcc aaccaccgca cgctggagtt cgctaaatat ctggcgaaca
     4081 aaaatgtgaa ggtgtggatc cgctacgttg ttgtcccagg ctggtctgac gatgacgatt
     4141 cggcacatcg cctcggtgaa tttacccgtg atatgggtaa cgttgagaaa atcgaacttc
     4201 tcccctacca cgaactgggc aaacacaaat gggtggcgat gggtgaagaa tacaaactcg
     4261 atggtgttaa accaccgaag aaagagacca tggaacgcgt gaaaggcatt cttgagcagt
     4321 acggtcataa ggtaatgttc taatttcctc gtaaagcggc aactttattg agttgccgct
     4381 tttttattcc gcgcttcatt ctgctacagc gtttttaaca catcttatag tgcatctacc
     4441 atatcccgta cctgagataa taacgactga taatagctcg cgtgaagttc ttccccatcg
     4501 aa
//
LOCUS       DBBBSB010000219         4461 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.223, whole genome shotgun sequence.
ACCESSION   DBBBSB010000219 DBBBSB010000000
VERSION     DBBBSB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.223"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..143
                     /locus_tag="ACIUS0_004111"
     CDS             <1..143
                     /locus_tag="ACIUS0_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGB0221620.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACIUS0_004112"
     CDS             127..486
                     /locus_tag="ACIUS0_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221621.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACIUS0_004113"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACIUS0_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HGB0221622.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACIUS0_004114"
     CDS             707..1141
                     /locus_tag="ACIUS0_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221623.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..2181
                     /locus_tag="ACIUS0_004115"
     CDS             1063..2181
                     /locus_tag="ACIUS0_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221624.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            2530..3702
                     /locus_tag="ACIUS0_004116"
     CDS             2530..3702
                     /locus_tag="ACIUS0_004116"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGB0221625.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(3756..>4461)
                     /locus_tag="ACIUS0_004117"
     CDS             complement(3756..>4461)
                     /locus_tag="ACIUS0_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221626.1"
                     /translation="CEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADL
                     AIDNTGTRPVTVVINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQ
                     PSAERPYWITGANCEVVDIGCVFTETTSKVTSASALSVTRSGKINANGSIDVATGVSS
                     VNVVSTGVYDVSFSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLS
                     GAGISSSFAFSITRLI"
BASE COUNT         1408 a          944 c          957 g         1152 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatgcaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggagactc
     1381 agggcagaag catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataga tatgcagcgc cgggtggcat ggataaaccc ggaagagagt
     1741 aaatcaaacc gcgcaattgg cgttgcgctg aatgatactg catgtcgcgt attgaaaaaa
     1801 caaatcggga atcatcaccg ttgggtattt gtgtacaagg aaagctgtac caaaccagac
     1861 ggaacgaaag cgccaacggt caggaagatg cggtatgacg caaacacagc ctggaaagcg
     1921 gcgctgagac gagcaggtat tgatgatttc agatttcacg acttgagaca cacctgggca
     1981 agttggctgg ttcaagccgg agtcccgttg tcagtgttac aggaaatggg aggctgggag
     2041 tctatcgaaa tggttcgtcg atatgctcac cttgcaccta atcaccttac cgaacacgca
     2101 cggcaaatag actcgatcct gaacccatcg gtcccaaatt cgtcccagtc aaaaaataag
     2161 gaaggtacta atgatgtgta acttattgat ttaaatggtg ccgataatag gagtcgaacc
     2221 tacgaccttc gcattatgaa tctgtagcac caatcataac tatctgtttt agcaagcatt
     2281 aaccgcattc actaagctat agttgatgac acaaacagaa agttgatgca tgatgttgcc
     2341 atatatatgt cacaaatacg gcacaacgat cttcaaacat atagccacac attcacagaa
     2401 gagcacaaag ccttgcaatc cagtgcaaag cttggtgtgt ctccgttttg tttcatcagt
     2461 tacacataac tatatgatca ttaactaaaa gtcagtatac ttggagaaat tttaattgcg
     2521 taatagagca tgcatatgat tgaaattaac agtttattat taataacatc cgtgatattg
     2581 atgtcgttat tagctgtggg gttatttgat aaaatttccc caataaatct tgttgagcac
     2641 ggcaggaata atcaaataga tggaatgcgg gggttcttag caattttcgt gcttattcat
     2701 cacgcagcaa tttggaatgg atacttgtca tctggagtat gggaagcacc ttcatcaaat
     2761 ctgttagcaa atttaggcca agttggtgtg tcattctttt ttatgattac tggttatctg
     2821 ttcttttcaa agattatctc tggagatcag gactggacaa ggctttatgt atcaagatta
     2881 ctacgattaa ccccaatgtt catagttagc ctatgtctca tttttatcat tgtaggtttt
     2941 aagtctggat ggagaatgca ggtatccaca gaagagcttt ttgtgtcaat aatgaagtgg
     3001 ttgccattca ctgcgctagg tatgccgaac attaatgacg taaaagattc atttactatc
     3061 aatgccgctg taacatggac acttgtatat gaatggttct tttatttttc tcttccggta
     3121 atttccgcgc tcataaaaag aaaagtcagt atttacatgg ttatgattag cgcaatatcg
     3181 ctatttgttt tcattttatt tttcagtaaa atacacatag catcatttct ttttgggctc
     3241 ctggcatttt tgctaaataa atcaaagata gtcaatggaa ttgccaaagc aaaagtaaca
     3301 ccaattataa taactgcgat aatggttttt gaaatgacgt atttcaaaac aacttacgca
     3361 ccgctaccac tgattctttg tggaataaca tttatcatta tcgcatcagg ttgtgatttg
     3421 tatggaatat taagattaaa tataaccaga aagctaggag aaactaccta cagcgtttac
     3481 cttctgcatg ggatattcct ttattgccta atgacgtgga ttattcctaa taattacact
     3541 gaaaatacat ttatcatact ggtaagcaca actgcatttc ttattacgtt cacatcatgc
     3601 ctaactttta aattaataga gacgccattc atcaaattaa caaaacaaac cacaacttta
     3661 gtaaaagaat taatacccac attaacaaac aacaatcaat aaacaataaa cataagccgc
     3721 cctacatata tgtccaaatg gggcggcgaa tgtatttata ttagtctggt aatgctaaat
     3781 gcaaacgaac tggagattcc tgccccgcta agcgtattgc gcagggtcac cctaaatgta
     3841 tctacgccaa tataagtaac atcacaacta actgaatcag gcgcagatat aggtgttatc
     3901 tgcacaatat aaccgctagc aactgctgcg agcggatgag aaaagctaac atcatacaca
     3961 cctgtagaaa ccacgttaac cgatgaaaca ccagtagcaa cgtcgatact accattggcg
     4021 ttaatttttc ccgagcgtgt gactgacaat gcagaagccg atgttacctt actggttgtt
     4081 tctgtaaaaa cacacccaat gtcaactacc tcgcaattag ctcctgttat ccagtatggt
     4141 cgttctgcac ttggctgata atcccctgcg ctctggaacg ttgtgccgtt gagtatgact
     4201 gtaacttttc ccccacctga gcttgttact tgaatattcg tgtgcgtgta tcttgcactg
     4261 ctcacgcgat gaaaatttcc accgtttata accacagtta cagggcgcgt cccagtatta
     4321 tctatagcaa ggtctgcccc cccggcatta gcctcaaagt atgggttgtt taataccaaa
     4381 gcgcacgaac cgttaaggcc atttatagct aattgcgcac cacctgtact ggaatccccc
     4441 tgagtaccat ttccttcaca a
//
LOCUS       DBBBSB010000220         4332 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.224, whole genome shotgun sequence.
ACCESSION   DBBBSB010000220 DBBBSB010000000
VERSION     DBBBSB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.224"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            56..2572
                     /locus_tag="ACIUS0_004118"
     CDS             56..2572
                     /locus_tag="ACIUS0_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221627.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            2648..3103
                     /locus_tag="ACIUS0_004119"
     CDS             2648..3103
                     /locus_tag="ACIUS0_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGB0221628.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            3246..3335
                     /locus_tag="ACIUS0_004120"
     CDS             3246..3335
                     /locus_tag="ACIUS0_004120"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGB0221629.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            3514..4332
                     /locus_tag="ACIUS0_004121"
     CDS             3514..4332
                     /locus_tag="ACIUS0_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGB0221630.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         1056 a         1101 c         1164 g         1011 t
ORIGIN      
        1 tcagtgccac attcactggc attcacaata acatgatatt catcacggag tgactatgtt
       61 acagatagtc ggcgcgctga ttctgctgat cgcaggattt gccattcttc gccttttgtt
      121 cagagcatta accagcacag cgtctgcgct ggcagggttc atattgctgt gtctgttcgg
      181 tccggcttta ctggctggct atatcactga acgcataacc cggttattcc atattcgctg
      241 gctggcaggc gtatttctga cgattgccgg aatgatcatc agcttcatgt ggggacttga
      301 tggtaaacat atcgcactgg aggctcatac ttttgactct gtaaaattta ttctgaccac
      361 cgctctcgcc gctggtctgc tggctcttcc cgtgcagata agaaccattc agcagaacgg
      421 gctcacacct gaagatatca gcaaggaaat taacgggtat tactgctgtt tttatactgc
      481 ttttttcctt atggcgtgtt ctgcatacgc accattgatc gcattgcagt tcgatatttc
      541 accctcactg atgtggtggg gcgggttgtt gtactggctg gctgcattag tgacgctgct
      601 atgggcggcc agccagatcc aggcgctgaa aagactgacc agtgccatca gccagacact
      661 ggaagaacaa ccggtgctca acagtaaatc gtggctgagc agtttgcaaa acgattacag
      721 ccttcctgaa acgctgacgg agcgcatctg gctgacactc atttcacaac ggatttcccg
      781 gggagaactg agggaatttg aactggcaga cggaaactgg ttactgaaca atgcctggta
      841 tgaaagaaac atggcagggt ttaacgaaca gttgaaagag aacctgtcat tcacacctga
      901 tgaactgaaa acgctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt
      961 tctcgatcgt tgcctggacg gcggtgactg gtaccccttt tcagaaggcc gccgttttgt
     1021 atcattccac cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca
     1081 tcatgccccg gaaaatcata agccggctcc ggaatggtac tgctcctctc tttgtcacga
     1141 aacagaaaca ctgtgtcagg acatttatga acgttcttac accggtttta tttccgatgc
     1201 aacggcgaat ggtctgattc tcatgaaact gccggaaacc tggagtacaa atgagaaaat
     1261 gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga
     1321 cagagtccgt ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga
     1381 cagactggtc agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt
     1441 cggtgcggca ttcgacggac aaaacggaca gtatcgttac atgggaaatc atggtcccat
     1501 gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga agaataagat
     1561 ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc tgattcgtcg
     1621 gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt
     1681 cacttatgat attgccgagg ggtcggttgt cagtctggcg gccggaggga tcagttttgc
     1741 cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc
     1801 tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca
     1861 acttcaccgg ctcactgttg ttcagggtat gctgaagcat attgattttt cgacggccag
     1921 cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa
     1981 caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccagccc
     2041 tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca ggaatcttgc
     2101 cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg ggatattgtt
     2161 cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg
     2221 aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt
     2281 caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac
     2341 cggacatgag attgaaaatc tgaacgcgaa tctggcccgt gttatcgatc agaatgctct
     2401 ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc
     2461 actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg
     2521 caagatggtt aaccggctgg aagaagcatt ccccccggag ctcccggcat aacgtgtgtt
     2581 ctgctccttt taggttttat cacctgattt gccagtgtca tcgtcatgac tcagagaggt
     2641 aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac
     2701 gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca atacagatac
     2761 gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg gcggaaacgg
     2821 tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg gtcagacgct
     2881 ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt aatgccacgc gaaccacaga
     2941 cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca ttaaagcaac
     3001 tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcgatc cccaggacta
     3061 ccatccactg ctgattaccg tttccggaaa aaatgttaac tgatccctga agtcagtttc
     3121 atgcttcagc ctctgccaca acgtatggca gaggctattt atcaggagaa accagatatg
     3181 ccgggttaca cacagagtat gtcctggctg aaggatcttt ctcatacggg caggcggttg
     3241 ctgtcataac cgcttaccgc aatgtgttta tccaggatga cccgggaatg catttccgtc
     3301 gggttatccg caacgcggaa ggtcagcgac gctgacgcag ccggaactct gaagctgatg
     3361 ccggaaaaca gcttaatgcg tggctcgcca gtggagggct tctcagacaa taaacttcat
     3421 tgatttcatc catcaggccg cgtcttctcc gggagacgcg gcttttttca tttgttgcac
     3481 cagtaaatct taaccaccga taaggagcaa agtatgcaat tagtcagtcg ttttggttat
     3541 gcgaaccaga tacgccgtga ccgtccgctg acacatgaag agctgatgca ccatgtgccg
     3601 gggattttcg gggaagagaa acacacgtcc cgaagccaga actatacgta catccccacc
     3661 atcaccgtac tggaaagtct gcagcgggaa ggcttccagc cctttttcgc ctgccagacc
     3721 cgtgtgcgcg acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc
     3781 ggagagataa acggagaaca tgtccctgaa attattctgc tcaactccca tgacggtacc
     3841 tccagctacc agatgctgcc gggttacttc agattcgtct gccagaacgg gtgtgtctgt
     3901 ggtcagtctc tgggggaagt gcgtgttccg caccggggaa acgtggtgga gaaagttatt
     3961 gaaggggctt acgaagtggt gggcgtgttt gaccggattg aggagaaacg tgatgccatg
     4021 cagtcgctgg tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac
     4081 cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc acgacgccgg
     4141 gaggattacg gtaaggacct gtggagtact tatcagacca tccaggagaa tatgctgaaa
     4201 ggcgggattt ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac
     4261 atcgacaccg atattaagct caaccgcgca ttgtgggtga tggcagaaac gctgctggag
     4321 agcctgcgct ga
//
LOCUS       DBBBSB010000221         4325 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.225, whole genome shotgun sequence.
ACCESSION   DBBBSB010000221 DBBBSB010000000
VERSION     DBBBSB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.225"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(210..956)
                     /gene="cutC"
                     /locus_tag="ACIUS0_004122"
     CDS             complement(210..956)
                     /gene="cutC"
                     /locus_tag="ACIUS0_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGB0221631.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(970..1536)
                     /locus_tag="ACIUS0_004123"
     CDS             complement(970..1536)
                     /locus_tag="ACIUS0_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGB0221632.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1752..3485
                     /gene="argS"
                     /locus_tag="ACIUS0_004124"
     CDS             1752..3485
                     /gene="argS"
                     /locus_tag="ACIUS0_004124"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGB0221633.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3527..3571)
                     /locus_tag="ACIUS0_004125"
     CDS             complement(3527..3571)
                     /locus_tag="ACIUS0_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221634.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3662..4150
                     /locus_tag="ACIUS0_004126"
     CDS             3662..4150
                     /locus_tag="ACIUS0_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGB0221635.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1148 a         1067 c         1045 g         1065 t
ORIGIN      
        1 ttatcagttt tagcaattta aatcccataa tcaatgtgaa tataaaaaat atttttagca
       61 caacgctatg cgcactgtca cccgctcaac agatcacggt tatcgttcgt tttttatact
      121 gctcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca
      181 tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc aatgattcct
      241 ttcatttcag caaccgccgc cccgtctacg atataacgcg aatactcgtc cgcgtgttca
      301 tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc
      361 gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg
      421 actcctgctc cagccataat gattggagca tcacgatggg caataagttc cataattttt
      481 gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc aatgcccaat
      541 tccgcaagat tattgagtgt atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa
      601 gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc
      661 ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc
      721 acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg
      781 cgaattatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag tacccccaac
      841 gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc tgcgccgttt
      901 tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa tttccagtaa tgccattttt
      961 gctccttaat tacgctgact gctcgctggc aacgatctct tcaatggacc acggatgaaa
     1021 cttaatagtt gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc
     1081 accttttggt gaactggttt taactgagat ccccggtagg ctgagtcctt catcagaaag
     1141 caatgccagc gcacgggcat tcagcgtttc tggatcaccc ggcagaacga tttcaatatg
     1201 ttcccaacct tcgtgtgggt aacgtttttc cccgggccac ggtagctcca cattggaaaa
     1261 ctgccagtgc gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt
     1321 gatcattttt tctgacaaaa gctcgccaca ctgttcaaaa ccgcgacgcc agcgctcagc
     1381 ggtgacgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatatccaa
     1441 ccccagacgg cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat
     1501 atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcagtaaa
     1561 agcgttaatt taccctgttg ccctgtgcca accaaccgct gatttcacgc cgcttctgat
     1621 gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag
     1681 tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt
     1741 aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg
     1801 cggcaggcgc gcctgcggat tgcgaaccgc agattcgtca gtcagcaaaa gttcagttcg
     1861 gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac
     1921 aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga
     1981 tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc
     2041 agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg
     2101 ttgactactc tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg cgctctacca
     2161 ttattggtga cacagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa
     2221 accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc
     2281 agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga
     2341 aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac
     2401 tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga
     2461 cgcagaacca gatcacttac gatcgtctca acgtgacgct gacccgtgat gacgtgatgg
     2521 gcgaaagcct ctacaaccca atgctgccag gaattgtggc ggatctcaaa gccaaaggtc
     2581 tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgtgtttaaa aacaaggaag
     2641 gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac accaccactg
     2701 atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca
     2761 tcgactcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcaggct
     2821 atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg ggtaaagacg
     2881 gcaaaccgtt caaaacccgc gcgggtggta ccgtgaaact ggcagacctg ctggatgaag
     2941 ccctggagcg tgcacgtcgt ctggtggcag aaaagaaccc ggatatgcca gctgacgagc
     3001 tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa
     3061 accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg
     3121 cgccatacat gcagtatgct tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta
     3181 acgaagagca actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg
     3241 cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa ggcacgccgc
     3301 atgtaatgtg tgcttacctg tacgatctgg ccggtctgtt ctctggcttc tacgagcact
     3361 gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac
     3421 tgacagcgaa gacgctgaag ctgggtctgg atacgctggg tattgagact gtagagcgta
     3481 tgtaatcgat ttttcgtgag agtgaagcct gatcagggtt agccgatcag gcttttttat
     3541 tgccatctaa atgtattctg aaaacggaca tgccattgtt ttctcactgt tgagtaagag
     3601 gccagaagcg taatatccag gcccaggaaa acgataacgg ttcaatttaa ggaataccga
     3661 agtgtttaaa tttcttgtat taacattagg cattatctct tgccaggctt acgcagaaga
     3721 tacggttata gtaaacgacc atgacacttc agccatcaaa gattgttggc aaaaaaattc
     3781 agatgatgat actgacatta acgtgatcaa atcatgcctg cgacaagaat acaatctcgt
     3841 cgatgcgcaa ttaaataaag cctatggtga agcttatcgt tatatagaac aagtgccacg
     3901 cacaggtgca aaaaaacttg atactgaaca acttaacttg cttaaaaaat cacagcgagc
     3961 ctggctggat tttagggaca aagaatgtga attaatcctt tcaaatgagg atgttcagga
     4021 tttaagtgac ccctattctg aatcagaatg gctatcatgt atgatcatac agaccaatac
     4081 gcgaactcgc cagttgcagc tataccgtaa ctctgaagat ttttatccaa gccctttgac
     4141 aagaggataa ttcacatctt tttggcatgt tttgttgcaa gctattcctg ataaatgatt
     4201 gcaacaaaac atccagcctt ttgactgagt tattaaacat actcgcgagc gcgtaatttt
     4261 ttgtctttca gcgataattc acaatcactt cattccgttg tacctttagc ggtggaatta
     4321 accgc
//
LOCUS       DBBBSB010000222         4209 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.228, whole genome shotgun sequence.
ACCESSION   DBBBSB010000222 DBBBSB010000000
VERSION     DBBBSB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.228"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..209)
                     /locus_tag="ACIUS0_004127"
     CDS             complement(<1..209)
                     /locus_tag="ACIUS0_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGB0221636.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACIUS0_004128"
     tRNA            complement(324..399)
                     /locus_tag="ACIUS0_004128"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACIUS0_004129"
     tRNA            complement(406..480)
                     /locus_tag="ACIUS0_004129"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACIUS0_004130"
     tRNA            complement(597..681)
                     /locus_tag="ACIUS0_004130"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACIUS0_004131"
     tRNA            complement(690..765)
                     /locus_tag="ACIUS0_004131"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACIUS0_004132"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACIUS0_004132"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGB0221637.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACIUS0_004133"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACIUS0_004133"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGB0221638.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACIUS0_004134"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACIUS0_004134"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGB0221639.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DBBBSB010000223         4198 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.229, whole genome shotgun sequence.
ACCESSION   DBBBSB010000223 DBBBSB010000000
VERSION     DBBBSB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.229"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACIUS0_004135"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACIUS0_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGB0221640.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACIUS0_004136"
     CDS             complement(995..1690)
                     /locus_tag="ACIUS0_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGB0221641.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACIUS0_004137"
     CDS             complement(1683..3110)
                     /locus_tag="ACIUS0_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGB0221642.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACIUS0_004138"
     CDS             complement(3121..3840)
                     /locus_tag="ACIUS0_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGB0221643.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DBBBSB010000224         4186 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.230, whole genome shotgun sequence.
ACCESSION   DBBBSB010000224 DBBBSB010000000
VERSION     DBBBSB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.230"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..193
                     /locus_tag="ACIUS0_004139"
     CDS             <1..193
                     /locus_tag="ACIUS0_004139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221644.1"
                     /translation="SIQSNRDWLSMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGIT
                     NIISIILQQAQDYDVAKIT"
     gene            complement(351..998)
                     /gene="ychE"
                     /locus_tag="ACIUS0_004140"
     CDS             complement(351..998)
                     /gene="ychE"
                     /locus_tag="ACIUS0_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGB0221645.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(1029..1082)
                     /gene="ychE"
                     /locus_tag="ACIUS0_004141"
     CDS             complement(1029..1082)
                     /gene="ychE"
                     /locus_tag="ACIUS0_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGB0221646.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1475..4150
                     /gene="adhE"
                     /locus_tag="ACIUS0_004142"
                     /gene_synonym="adhC"
     CDS             1475..4150
                     /gene="adhE"
                     /locus_tag="ACIUS0_004142"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGB0221647.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1022 c          959 g         1036 t
ORIGIN      
        1 ttcaattcaa tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag
       61 tagtgaaatg cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg
      121 cagtggaatt actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc
      181 gaaaataaca tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt
      241 gagaagaata tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa
      301 ataatgacga agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc
      361 aggccgggga aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc
      421 aatagcccca taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc
      481 cacggtgcca tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag
      541 aaaccaaaca gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc
      601 gcccctggcc ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc
      661 gcggtttctg atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc
      721 gacatcgcta tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata
      781 ccaaaaagtt gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca
      841 gacaggttgg ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg
      901 ctgataaaga cgggaataat ccctaccggg ttaaccagcg caaataaccc gatgaaaaat
      961 ttgaagtaaa cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc
     1021 ggataatgtt agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc
     1081 atttttcacc tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca
     1141 aaacatgcta atgtagccac caaatcatac tacaatttat taactgttag ctataatggc
     1201 gaaaagcgat gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt
     1261 acccagaagt gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact
     1321 ctaatgttta aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt
     1381 ttcatcggtt aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt
     1441 tactaaaaaa gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact
     1501 taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga
     1561 gcaagtagac aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact
     1621 cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa
     1681 ccactttgct tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct
     1741 gtctgaagac gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg
     1801 tatcgttccg accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa
     1861 gacccgtaac gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc
     1921 ggctgatatc gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg
     1981 gatcgatcaa ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct
     2041 gatcctcgcg actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc
     2101 tatcggtgta ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg
     2161 tgcagttgca tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga
     2221 acagtctgtt gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca
     2281 cggcggctat ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa
     2341 tggtgcgctg aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg
     2401 cttctctgta ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag
     2461 cgaaccgttc gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt
     2521 cgaagacgcg gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc
     2581 ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat
     2641 gaaaacagct cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta
     2701 taacttcaaa ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat
     2761 ctctgaaaac gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga
     2821 aaacatgttg tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat
     2881 cgcgctggat gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt
     2941 cctgttcaac aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga
     3001 aactgaagtc ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc
     3061 agaactggca aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat
     3121 ggacgccgcg aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct
     3181 ggcgctgcgc tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa
     3241 agcgaaaatg atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc
     3301 ggttgtaact gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc
     3361 ggatatggcg attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt
     3421 cggtggtctg gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga
     3481 gttctctgat ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc
     3541 ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat
     3601 cgcgggtatc gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact
     3661 gggttcccag ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat
     3721 tcgctacaat gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc
     3781 gcaggctcgc cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga
     3841 ccgtactgct gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact
     3901 gggtattccg aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt
     3961 ggataaactg tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc
     4021 gctgatctcc gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga
     4081 agatgaaact gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa
     4141 atccgcttaa tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DBBBSB010000225         4182 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.231, whole genome shotgun sequence.
ACCESSION   DBBBSB010000225 DBBBSB010000000
VERSION     DBBBSB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.231"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..186)
                     /locus_tag="ACIUS0_004143"
     CDS             complement(<1..186)
                     /locus_tag="ACIUS0_004143"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGB0221648.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACIUS0_004144"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACIUS0_004144"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGB0221649.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACIUS0_004145"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACIUS0_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGB0221650.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACIUS0_004146"
     CDS             3863..4144
                     /locus_tag="ACIUS0_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGB0221651.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DBBBSB010000226         4169 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.232, whole genome shotgun sequence.
ACCESSION   DBBBSB010000226 DBBBSB010000000
VERSION     DBBBSB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.232"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACIUS0_004147"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACIUS0_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGB0221652.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACIUS0_004148"
                     /pseudo
     CDS             complement(334..2351)
                     /gene="flhA"
                     /locus_tag="ACIUS0_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACIUS0_004149"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="ACIUS0_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3787..>4169
                     /locus_tag="ACIUS0_004150"
     CDS             3787..>4169
                     /locus_tag="ACIUS0_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGB0221653.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1039 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggca
//
LOCUS       DBBBSB010000227         4064 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.233, whole genome shotgun sequence.
ACCESSION   DBBBSB010000227 DBBBSB010000000
VERSION     DBBBSB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.233"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..662)
                     /locus_tag="ACIUS0_004151"
     CDS             complement(<1..662)
                     /locus_tag="ACIUS0_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HGB0221654.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACIUS0_004152"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="ACIUS0_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HGB0221655.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004153"
     CDS             complement(1339..1812)
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HGB0221656.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2144..3121
                     /gene="intI2"
                     /locus_tag="ACIUS0_004154"
                     /pseudo
     CDS             2144..3121
                     /gene="intI2"
                     /locus_tag="ACIUS0_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(3309..3548)
                     /locus_tag="ACIUS0_004155"
     CDS             complement(3309..3548)
                     /locus_tag="ACIUS0_004155"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HGB0221657.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1092 a          941 c          886 g         1145 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
     1981 cacattatct cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact
     2041 ctaccggtaa acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag
     2101 aataggctgt ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt
     2161 tttaaattct atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac
     2221 ttacctgcac tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat
     2281 gggcagtgaa gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat
     2341 aaacacgcag aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca
     2401 gccgttgggc gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat
     2461 ctctgcaaat gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt
     2521 tacgctgctg tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga
     2581 ttttgatttt gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa
     2641 cagcctactg cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct
     2701 tattcagcaa gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca
     2761 caaataccct tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct
     2821 ctgcaaccac ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg
     2881 aaaggcattg aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca
     2941 tacatttcgt cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt
     3001 gcaagaactc ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg
     3061 tcagcatttt gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta
     3121 aaagtagata aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa
     3181 agataaacaa tcatagaggg gataaatatg agccagttct accattgttc agataagttt
     3241 aagcaagata ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca
     3301 gacaatgcct aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt
     3361 tttctaagcg ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt
     3421 tccttataca tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta
     3481 ataatgcgac actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa
     3541 ggggtcattt gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga
     3601 ggctgctttc caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct
     3661 tagcgcatgt taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta
     3721 acacctgtac aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt
     3781 gttggtgagg cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc
     3841 agttggatat aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata
     3901 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3961 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     4021 cacccctccc agttcccaac tattttgtcc gcccacagct gccg
//
LOCUS       DBBBSB010000228         4060 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.234, whole genome shotgun sequence.
ACCESSION   DBBBSB010000228 DBBBSB010000000
VERSION     DBBBSB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.234"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            542..1333
                     /locus_tag="ACIUS0_004156"
     CDS             542..1333
                     /locus_tag="ACIUS0_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221658.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1719..1925
                     /locus_tag="ACIUS0_004157"
     CDS             1719..1925
                     /locus_tag="ACIUS0_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGB0221659.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2011..2583
                     /locus_tag="ACIUS0_004158"
     CDS             2011..2583
                     /locus_tag="ACIUS0_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221660.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2585..2869)
                     /locus_tag="ACIUS0_004159"
     CDS             complement(2585..2869)
                     /locus_tag="ACIUS0_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221661.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2840..2983
                     /locus_tag="ACIUS0_004160"
                     /pseudo
     CDS             2840..2983
                     /locus_tag="ACIUS0_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1254 a          927 c          777 g         1102 t
ORIGIN      
        1 taacaaagaa cattacagtc agtgtgtaca ttacagattt atattcaggt gttgtgggac
       61 tacattacag cagagagggg taaggacaga gttacaagta ctactagaga gaatatcact
      121 gcacagatac ttctatgagt atatctttat gagagatccg tctacaggat tgggttataa
      181 ggtgtattta caaggtataa tcacaggatc atttacatga ccatacttct gtaaataata
      241 ctaattaacc ttaattattt ttttatggta catgaactac ctgtagctgt aacgataaac
      301 aaatcaccca aaatgaaaaa tactggttat cacagttcag agaactcgta tgagtgtccc
      361 cctgagcaat gcttcatttt ctgtgattct gtgtgataga gttatactga gtacagtggc
      421 agtaacactg ctttccatca cttgaggata aaattcatta aaaaattaat tgcatgaatt
      481 aacaggtttt tttatgaaat gttatgcatc tcaaccaata tgattaacag aaaacttcat
      541 catgacaaca cgtataacgt tcagtatgac cagtgatgag acgctgcgaa ttgtggatga
      601 gtactgtcat acccacaaac tttcgcgctc taaggtaatc aacgcattac tcagcgccac
      661 agcacctgtc cttaatgata ttaactgtta ttatcaactt gcaggcgaac tgcagtcacg
      721 cttattaaat ggagtgtatc aacgggatct tcctcgcaaa cgcaatgtcg tcagtgcaga
      781 gaagtattgt ctggaaatct gggaaaacaa acttttcacc agaagaatcc tggagtttga
      841 cagttcaaat ggtgttctgt acgcgctgaa acataaaaga cactatcgcc gggataagat
      901 gattggaagg gttgaaagtc ggtgtatcaa agacatttgc gaataccaga tgcagctatc
      961 cggggagaag acgaaatatg cctgttttat ttatatcgaa agaacaatat acaatcacga
     1021 taatccatct gataaaatac ctgtaaaagc ggctgttggt aatgcagtca tcctgctggc
     1081 aaaggatgtg atatataacg agtacttttt tgatttgcgg cagtcatttt ttgtgagcgt
     1141 tacagatctg atggtctctg gcgcaaaagg tattccggag acacagacat atccggacgt
     1201 ttattgctgg atccctcttt tttccataaa ttccggcgtg ctcattaccc ctgtatataa
     1261 aattgaccct ctgaaacccg taacggtaaa aaaaccggat aagatcactg ttgtttgtaa
     1321 ttacagagaa taatttcagc cttctacctt caccgcgaca gtgtttacct ctgctttatc
     1381 ttccgttcag taaatacaaa ccaccgctct ggcaggtaca gggcgtggtt tatctccgat
     1441 actcccctgt gcagtaataa tcatcactga aaatactgat cccgtcacgg caaaacgcca
     1501 tcccatattc cggcaacaca aaatcatctt ccgtttatac cggattcact gacaaaacag
     1561 acaacctgta ttgcctgtac acaacagttt ctctcctggc aggaatattc ccctactcat
     1621 tttttacgtt tctccccggt tgttatgccg gtgaaggtgg tgcgtcgttt tcatcaccaa
     1681 cgccggttga gattaacaac atccggagga acattctcat gaatacccca gtttcactga
     1741 tggatgacca gatggtcgac atgtcgttta tcactcagct gaccggcctg accgataagt
     1801 ggttttataa actcatcaag catggagcct ttccgccccc catcaaactg ggccgtagct
     1861 cccgctggct gaaaagcgaa gtggaagcct ggctgcaggc gcgtattgca cagtcccgtc
     1921 cgtaattcct gaccgttatc cgttccaccg cagcaaaggc atgatccggc ccccggaggc
     1981 attttgctgc gcgctaaagg agagaatatt atgcaacaac ttgaaaacag gtgtgacctg
     2041 ctgctcatcc agcatcagaa atggatgaca agcgtgacga ggcttatcgt cgctcatgga
     2101 atggggtctc cgcatcttca tggttatcac cgtctgaccc tggcacactt ttttctgccg
     2161 gaaaaaggct ccgtcatttc ggtggccccc cagggactct atcaggtcgt caacccggga
     2221 acaccaccgt tcattcctgc cattcaggaa gggttaatga ccagcattca gacccatgaa
     2281 atcatgctgc ttacacattt taatctgggc ggcgttttac tcagtgagct acatcgtctg
     2341 ggtgaaaacc ggctggcaaa caggctgaac agcctgctcc ggcgtttcga cgatcgtgat
     2401 ctctaccaca cgctgatatg gctgtgctgg tatgatctga tgtgtgccca ctccatgcag
     2461 ccctggactg aagagttaaa gcataaatca catgcagagc ttgaaaactg ggctgtggcg
     2521 cgcaaacggg aaaagcgcga gctggaactg atgattgacg agtacctgtt atacgcctgc
     2581 tgattcataa cgttcccggg gttccttcct catcgttaca cacagccgga cggctatcta
     2641 tggctgtccg ccaaatgacc atcgccgcat ttcgttccgc tgaaaacggt acgtagtcag
     2701 tgataccgga atttccggag agggccgcag gtatattgct tctgtgcaat tgatgcttat
     2761 ttacattgga tgtagttatt tctgcatcca atgctatcgg aaagagatta accagcaaat
     2821 ggttcctcaa atcgtattga tatttacccg gcgtattgag caccaccatc tgacactccg
     2881 ttcccgcatc aaacgactgg accgaaaaac gatttacttt tcacgtgctg tggaaattca
     2941 cgaaaaaatt attgggtcat tcatcgaaaa acatatgttc tgatgactgg agtcatcctc
     3001 cagaaatata cgtcaactga acaatatcga ttttttatgg gattctatac acttttccat
     3061 atccctctga aaatcagtca tcagctcacc aacacctctt ctgaaacttg tattaaactt
     3121 attgcttaaa tagatgatat aacatctctt ccattgcact gaatcggcga aaactgccgt
     3181 ttcaataatc tcaacaatat tatttatcgg ctaactttat cagtaaacaa tggaatgtca
     3241 cactgatgaa atgaacattc agagtaagat gataaaattc atataaacgc cattgctgaa
     3301 tattatttct ttcatacaga taaacgataa aatacaaaag ggtatatttc tcaatgtaac
     3361 agtcaattat tcatctccac tggcgagcac ggtcagcaca caaacaaagt tagtcctcct
     3421 gcttaatcat tgcgtatcag aaactaaaaa agaaaaaatt ctgcagacaa cttaacaatc
     3481 ttcatcacat ttcctgaaga cagacatatc cacaaacggt agtcagcgtt gaattaacaa
     3541 gtcttacaga gctatacgta gcttataaag ccactcaatg ttcatcatca cccgactact
     3601 tatctggaat accaccagta taaactcatc aataaaaaca cctgttgtta aaataagaat
     3661 actgtcagca gtaaattatt aaaacaacat ctgtccacac tatctgttct tttaacgcca
     3721 acggcagcga aaatagtgca tcacagcctt cttttttctt tcgccaccac tcagacaaac
     3781 acaacactca cacccacacc acaaaacaga atcaaaacat aaaattaaca ataacagtca
     3841 aaaggaaaac acagaatatt gaggtggact gtcaccagga cagcgcatac gacgcatgtg
     3901 atcagcacat cccgttcttc gctggcatcc ccaaaatgca gaacaaacaa tcataacatt
     3961 atgaatataa aagaaaatat gatttttcac catgaaaaaa acatcaatgt atctctgtat
     4021 aaaaataatg ataacatgac ataaacattt aaaaacataa
//
LOCUS       DBBBSB010000229         4042 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.235, whole genome shotgun sequence.
ACCESSION   DBBBSB010000229 DBBBSB010000000
VERSION     DBBBSB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.235"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(126..284)
                     /locus_tag="ACIUS0_004161"
     CDS             complement(126..284)
                     /locus_tag="ACIUS0_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGB0221662.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACIUS0_004162"
     CDS             complement(886..1077)
                     /locus_tag="ACIUS0_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGB0221663.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACIUS0_004163"
     CDS             1142..1465
                     /locus_tag="ACIUS0_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGB0221664.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACIUS0_004164"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACIUS0_004164"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACIUS0_004165"
     CDS             2143..2673
                     /locus_tag="ACIUS0_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGB0221665.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACIUS0_004166"
     CDS             2680..2913
                     /locus_tag="ACIUS0_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGB0221666.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACIUS0_004167"
     CDS             complement(3459..3851)
                     /locus_tag="ACIUS0_004167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221667.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DBBBSB010000230         3976 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.236, whole genome shotgun sequence.
ACCESSION   DBBBSB010000230 DBBBSB010000000
VERSION     DBBBSB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.236"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..593)
                     /locus_tag="ACIUS0_004168"
     CDS             complement(<1..593)
                     /locus_tag="ACIUS0_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HGB0221668.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACIUS0_004169"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACIUS0_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGB0221669.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACIUS0_004170"
     CDS             complement(1422..1961)
                     /locus_tag="ACIUS0_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0221670.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACIUS0_004171"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACIUS0_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGB0221671.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="ACIUS0_004172"
     CDS             complement(3055..3651)
                     /gene="fimE"
                     /locus_tag="ACIUS0_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGB0221672.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         1103 a          932 c          830 g         1111 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt tgttggctta
     2761 atattctatt attatcttta tttatagatt tttatattgc atgaggtggt ttttggagag
     2821 aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag cggaggtgat
     2881 gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc tttttgtcgt
     2941 tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat ggacagtttg
     3001 gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt taaatcaaac
     3061 ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc cggcaaaacg
     3121 agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc
     3181 ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa
     3241 catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg
     3301 ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg
     3361 gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc
     3421 aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc
     3481 ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg
     3541 ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc
     3601 ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt
     3661 actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta
     3721 gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta
     3781 aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt
     3841 caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata aagaaaaaag
     3901 tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt ttatttattt
     3961 tgaagaacat aaatca
//
LOCUS       DBBBSB010000231         3966 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.237, whole genome shotgun sequence.
ACCESSION   DBBBSB010000231 DBBBSB010000000
VERSION     DBBBSB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.237"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..224
                     /locus_tag="ACIUS0_004173"
     CDS             <1..224
                     /locus_tag="ACIUS0_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGB0221673.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACIUS0_004174"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACIUS0_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGB0221674.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACIUS0_004175"
     CDS             518..1108
                     /locus_tag="ACIUS0_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HGB0221675.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACIUS0_004176"
     CDS             1348..1608
                     /locus_tag="ACIUS0_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGB0221676.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACIUS0_004177"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACIUS0_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGB0221677.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACIUS0_004178"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACIUS0_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGB0221678.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACIUS0_004179"
     CDS             3457..3648
                     /locus_tag="ACIUS0_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221679.1"
                     /translation="MAENGYGLARLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3878..>3966)
                     /locus_tag="ACIUS0_004180"
     CDS             complement(3878..>3966)
                     /locus_tag="ACIUS0_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221680.1"
                     /translation="RFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1074 a          984 c          986 g          922 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcaaggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctccatat gaacggaacc
     3781 gacgattaaa ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt
     3841 ttccctcaaa atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg
     3901 ccagcggctc atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa
     3961 aacgca
//
LOCUS       DBBBSB010000232         3806 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.238, whole genome shotgun sequence.
ACCESSION   DBBBSB010000232 DBBBSB010000000
VERSION     DBBBSB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.238"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            437..2089
                     /gene="ushA"
                     /locus_tag="ACIUS0_004181"
     CDS             437..2089
                     /gene="ushA"
                     /locus_tag="ACIUS0_004181"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HGB0221681.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="ACIUS0_004182"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="ACIUS0_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="ACIUS0_004183"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="ACIUS0_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3595)
                     /locus_tag="ACIUS0_004184"
                     /pseudo
     CDS             complement(2596..3595)
                     /locus_tag="ACIUS0_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          875 g         1001 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctgtaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DBBBSB010000233         3738 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.239, whole genome shotgun sequence.
ACCESSION   DBBBSB010000233 DBBBSB010000000
VERSION     DBBBSB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.239"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            108..2105
                     /locus_tag="ACIUS0_004185"
     CDS             108..2105
                     /locus_tag="ACIUS0_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HGB0221682.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACIUS0_004186"
     CDS             2168..3445
                     /locus_tag="ACIUS0_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HGB0221683.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DBBBSB010000234         3727 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.240, whole genome shotgun sequence.
ACCESSION   DBBBSB010000234 DBBBSB010000000
VERSION     DBBBSB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.240"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1381
                     /gene="ospC1"
                     /locus_tag="ACIUS0_004187"
     CDS             <1..1381
                     /gene="ospC1"
                     /locus_tag="ACIUS0_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HGB0221684.1"
                     /translation="TQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDEKNLKDSANIIK
                     SFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDRLSKHYISEIRE
                     KLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSEGICTKRNTYAD
                     DIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSPYGYMTLTDHFD
                     NAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFI
                     RKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEA
                     VTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYD
                     IEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKY
                     GATSDNKYI"
     gene            complement(1485..1634)
                     /locus_tag="ACIUS0_004188"
                     /pseudo
     CDS             complement(1485..1634)
                     /locus_tag="ACIUS0_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1710..3407
                     /gene="ospD3"
                     /locus_tag="ACIUS0_004189"
     CDS             1710..3407
                     /gene="ospD3"
                     /locus_tag="ACIUS0_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HGB0221685.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            3524..3643
                     /locus_tag="ACIUS0_004190"
                     /pseudo
     CDS             3524..3643
                     /locus_tag="ACIUS0_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         1292 a          655 c          691 g         1089 t
ORIGIN      
        1 tacccaatgc aatatagatt ctatggataa cagattacat acattgtttc caaaagtgac
       61 atcagtgcga aacgctgcac aacaaactat gccagatgaa aaaaatttaa aagatagtgc
      121 aaatattatt aaaagtttct ttaggaaaac tatagcagca cagagttata gtagaatgtt
      181 ctctcaaggc tctaacttta aatctttaaa tatagcaatt gatgcaccat cagacgctaa
      241 agcctcattt aaggctattg agcaccttga cagattatcg aagcattata tatctgaaat
      301 aagggaaaaa cttcatcctc tttctgcaga ggaactcaat ttgctttcgc taattattaa
      361 ttctgattta atcttcagac atcaaagtaa ttctgatttg tctgataaaa ttttaaacat
      421 taagtcattc aataaaattc agtctgaagg aatatgcaca aaacgaaaca catacgctga
      481 tgatataaaa aaaatagcta atcatgactt tgtgtttttt ggcgttgaaa tctctaacca
      541 tcagaaaaaa caccccctga atacaaaaca tcacactgtt gattttggtg caaatgcgta
      601 tatcattgat catgactctc catatggata tatgacatta accgatcact ttgataatgc
      661 tattccacct gttttttacc atgagcacca atcattttta gataaatttt cagaggttaa
      721 taaagaagtt agtcgatacg tacatggaag taaaggaatt atagatgtac caatattcaa
      781 tactaaagat atgaagttag ggctcggatt atacctgatt gactttatta gaaaaagtga
      841 agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt gcccctgtgg atctggatag
      901 aatcataaac tttgtttttc agccagagta ccatatacct aggatggtaa gtacagaaaa
      961 cttcaaaaaa gttaagatta gagaaatatc cttagaggag gctgttacag catctaatta
     1021 cgaagaaatt aacaagcagg tcactaacaa aaaaattgct ctccaggctc tttttctttc
     1081 gattactaat caaaaagagg atgtcgcctt atatatatta tctaattttg agataactag
     1141 acaagatgtt atttccataa agcatgagtt gtatgatatt gagtatctac ttagcgctca
     1201 taattcaagc tgtaaagtac ttgagtattt tatcaataag ggattggttg atgtaaacac
     1261 aaagttcaaa aaaactaata gtggggattg tatgttggat aacgcaataa aatatgagaa
     1321 tgcagaaatg ataaaactat tattgaaata tggtgcaaca tctgacaata aatatattta
     1381 atcaaaattg aatatcgttt agcaaaaggc agtatttgca caaaatatcg atatattgtt
     1441 tattgtcagt atggctcaat gtgataatag ttggaaagtt tgatgggttt cgccccgttg
     1501 tagcggtagt cgaactggaa ggtcttcagg cactgcttac agcgatagag cagcccccct
     1561 gaactggaat ggccgttccg gatacccccc tgagtttcag agtaacggtg acaaaccaca
     1621 tcaatctttg ccatcaatca tccaaagggc aaagagtaca acaacactaa gtctgcgtca
     1681 caacccatca atgaaaggaa tatatacata tgccatcagt aaatttaatc ccatcaagga
     1741 aaatatgttt gcaaaatatg ataaataaag acaacgtctc tgttgagaca atccagtctc
     1801 tattgcactc aaaacaattg ccatattttt ctgacaagag gagtttttta ttaaatctaa
     1861 attgccaagt taccgatcac tctggaagac ttattgtctg tcgacattta gcttcctact
     1921 ggatagcaca gtttaacaaa agtagtggtc acgtggatta tcatcacttt gcttttccgg
     1981 atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa ggctattaat gtgcctgcta
     2041 ttatttattt tgttgaaaac ggttcatggg gagatattat tttttatatt ttcaatgaaa
     2101 tgatttttca ttccgaaaaa agcagagcac tagaaataag tacatcaaat cacaatatgg
     2161 cattaggctt gaagattaaa gaaactaaaa atggggggga ttttgtcatt cagctttatg
     2221 atcccaacca tacagcaact catttacgag cagagtttaa caaatttaac ttagctaaaa
     2281 taaaaaaact gactgtagat aattttcttg atgaaaaaca tcagaaatgt tatggtctta
     2341 tatccgacgg tatgtctata tttgtggaca gacatactcc aacaagcatg tcctccataa
     2401 tcagatggcc tgataattta cttcacccca aagttattta tcacgcgatg cgtatgggat
     2461 tgactgagct aatccaaaaa gtaacaagag tcgtacaact atctgacctt tcagacaata
     2521 cgttagaatt acttttggca gccaaaaatg acgatggttt gtcaggattg cttttagctt
     2581 tacaaaatgg gcattcagat acaatcttag catacggaga actcttggaa acttctggac
     2641 ttaaccttga taaaacggta gaactactaa ctgcggaagg aatgggagga cgaatatcgg
     2701 gtttatccca agcacttcaa aatgggcatg cagaaactat caaaacatac ggaaggcttc
     2761 tcaagaagag agcaataaat atcgaataca ataagctgaa aaatttgctg accgcttatt
     2821 attatgatga agtacacaga cagatacccg gactaatgtt tgctcttcaa aatggacatg
     2881 cagatgctat acgcgcatac ggtgagctca ttcttagccc ccctctcctc aactcagagg
     2941 atattgtaaa tttgctggcc tcaaggagat atgacaatgt tcccggactt ctgttagcat
     3001 tgaataatgg acaggctgat gcaatcttag cttatggtga tatcttgaat gaggcaaaac
     3061 ttaacttgga taaaaaagca gagctgttag aagcgaaaga ttctaatggt ttatctggat
     3121 tgtttgtagc cttgcataat ggatgtgtag aaacaattat tgcttatggg aaaatacttc
     3181 acactgcaga ccttactcca catcaggcat caaaattact ggcagcagaa ggcccaaatg
     3241 gggtatctgg attaattata gcttttcaaa ataggaattt tgaggcaata aaaacttata
     3301 tggaaataat aaaaaatgaa aatattacac ctgaagaaat agcagaacac ttggacaaaa
     3361 aaaatggaag tgattttcta gaaattatga agaatataaa aagctgaata ttatctcgtg
     3421 gtgatacact gaatttgagg taaccggctc atttaaaccg tctggtctgt tacctccggt
     3481 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accggacctg
     3541 gatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg tggccggacg
     3601 tcgcctgggc gtaccaaaat caactgtctg tggtatgttc gtgtgaaccg cccccggtat
     3661 cccgctcatg ctggcgaacc cagacacgca gtgaaccgcc ccgggtttcc tggagagtgt
     3721 tttatct
//
LOCUS       DBBBSB010000235         3650 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.241, whole genome shotgun sequence.
ACCESSION   DBBBSB010000235 DBBBSB010000000
VERSION     DBBBSB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.241"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..401
                     /locus_tag="ACIUS0_004191"
                     /pseudo
     CDS             1..401
                     /locus_tag="ACIUS0_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            434..834
                     /locus_tag="ACIUS0_004192"
                     /pseudo
     CDS             434..834
                     /locus_tag="ACIUS0_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1418..2134
                     /locus_tag="ACIUS0_004193"
     CDS             1418..2134
                     /locus_tag="ACIUS0_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221686.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2368..2496
                     /locus_tag="ACIUS0_004194"
                     /pseudo
     CDS             2368..2496
                     /locus_tag="ACIUS0_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2686..3090
                     /locus_tag="ACIUS0_004195"
     CDS             2686..3090
                     /locus_tag="ACIUS0_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221687.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            3153..3650
                     /locus_tag="ACIUS0_004196"
                     /pseudo
     CDS             3153..3650
                     /locus_tag="ACIUS0_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1030 a          759 c          871 g          990 t
ORIGIN      
        1 cgcttcctgt ggcagcacag cgataaatgg atgaccgaat ccaggcagga aaagctgata
       61 tggctgcgtg cacagatgaa gctgacgagc ctgtgctggg cgctgaaaga actggcaaag
      121 gatatctgga gcagaccatg gagcgaggaa agacgcaacg actggcagag atggctgcgg
      181 ccaacagtga cgtccccatg atgaagaatg tggctaaaac gatagggaaa aggttgtacg
      241 gtatcctgaa cgccatgcgc catggcgtat caaacggaaa tgcggaagca ctgaacagca
      301 agatcaggct gctgagaata aaagccaagg gctaccgcaa ccgggagcgc tttaagctgg
      361 gagtgatgtt ccactatggg aaactgaata tagcgttcta agccttccca ccatgatcgg
      421 ggaagcccca gatatgaaca ggcacattca gagttacttt aaagagctta ctgaacgtct
      481 gaggtgttag agccatgact gccgcagcat tgctggcgga aatgcctgaa tcgagcagcc
      541 tctcgagacg tgaaatcagc gcacttgtcg gtgttgctca ggtaaaccgg gactcgggaa
      601 ctctgcgcgg tcgacgaacc attttcggtg attgtgctgg ggaagaacag ctctgtacat
      661 ggcggcgctt gaggcccccc ccggtttaat ctggtgataa aggcgtttta catgcgccta
      721 cttgcagccg gaaacgccaa aaaagtggca ctggttgcct gtatgcgtaa gttgctgacc
      781 attatgaacg caatgctcag aaaaaacgaa gaatggaatg aatcgtattt atgaaaactg
      841 gattgtgggc cataaatatt ccaaaaagat agcccgctcc gtttattacc ggaacgggcg
      901 agagcatgac attgtctgtt attataatga gactgctgat tctacttatt tttaatttca
      961 agaaaagcgt tttctacaaa gaattatgag atttaatgtt ttgtttagac aatattatca
     1021 ttctgttcaa taatgaaagt cttcatcaac aacaagtaaa aacaacgtcc ctctcatcga
     1081 accggacttg cagatttccc gcatccggct ctcttgcaac acatcagacc ttcacgttcg
     1141 ggcggttatg gggaaggtcc agcaagctgt atgagcccta actgtttgaa taccagtaga
     1201 ttaccagaaa cttctatcga ttgaagacat taccaactag cgttactaaa tgttgaccaa
     1261 gttttgttca cttgtctggt tttgatagta gcattctgca gagtattttg atatgataaa
     1321 tatgcattaa caatgatatt attgttaatt tcctagcatg aaaattaaaa gatcaacttt
     1381 tatctcgaat attttttaca ttatcagtta gtttcttatg aatgacaatt cattgcttag
     1441 aaattcttca ttattcatcg cctatatggg atgtgtaggg tgggtcagtg cttattctta
     1501 tggctgggga acctcttttt actatggatt tccatggtgg gttgttggtg caggtttgga
     1561 tgatgttgcc agaagtttac tctatgctat catagtaatg ggtatacttt ttaccggttg
     1621 ggggattggt attttatttt ttcttcttat aaagaaaaga agtaaaatac aagacttgag
     1681 tttttttaga ctattttttg ctattaccct actgtttttc cctgtaatat tcgaactttt
     1741 aattctcaaa caatatttca tacttccatt atcactttca ttcattatta gttcattggt
     1801 tatttcaata ataattcgta tatatggacg gatcttttct gtatcatgtt tttcagatat
     1861 cccttttgtt agagaacacc gcattaagtt aattatggct ggttttttgg tttatttttg
     1921 gtttttttcc tttcttgttg gctggtataa acctcaatta aaaaaagaat atcagatgct
     1981 ttgttataat aatagttggt attatgttct tgctcgttat gattcacggc tcgttttatc
     2041 tagttcattt aaagatgaca gtaacagatt cttaatattt aatacagaac agtcgggatt
     2101 ttatgaaatc aatgatgttt atgtcagaaa gtaataatcc tctcccacaa agtgaattaa
     2161 atgaatatat aatgcgttcg aagtatatgt attgagctgt catactgaga aacaaacctt
     2221 agggcagcac aattaaagcc agaaacaact atgcaagaaa gttactaaat aagtgctcgg
     2281 aacagtaaaa cactcagggg ggactcagtc cggattatgt aatcagataa attgccaaac
     2341 atcacaaatc accagccgga ctgagtgatg ccgagcattg caccaatacc ccgtgatgaa
     2401 cgatgcctga tgcagaaagc tccctataaa acaaagaaca aaaactacga ccggcgattc
     2461 accgtcatac tgatgctgca tcgtggtgac actgtctgtc actttcatgt tgactgaaca
     2521 actctgctgc gaagaaggtg taccgccggc gggatttgat tgtccggcgt ctagcttcca
     2581 cttccctgat ggaactgaaa gttgttatgg ccggtcagct tgccctgtcc gcaggacatg
     2641 aggtgctgtg acggacagca ccagtaacgg aaagtgacaa tgaaaatgaa tactgcgctg
     2701 atagttgcac taatgtgtat gtggtatgcg gtacccgctg ctgcaaaaga gactttactg
     2761 gctatgccac gtaattcaac agaacattgc tatgcggaga ttaatgtgca cggaccatat
     2821 ggggtgtatt tccgagtggt cccgcatccc cccgggggaa agagctgggt cgaatgtaac
     2881 agtgactact attactctga taaaccaccg ggagtacaga tactcggaac ccgtgcaggg
     2941 tgtcgggtat acggaatctg cgggaccacc tcgacactgc atgtggctgg aagaggggtt
     3001 gtgtgtatta aaaatatatg cagtccacgt gggatgataa ttcacaggat acggaaacgt
     3061 cctgtagtgg ctgtaagcga tgagatgtga tcttacccag caatagtaga cacgcggcta
     3121 agtgagtaaa ctctcagtca gaggtgactc acatgacaaa aacagtatca accagcaaaa
     3181 aaacacgtaa acagcattcg cctgaatttt gcagtgaagc cctgaagctt gctgaacgca
     3241 tcggtgttgc tgccgcagcc cgtgaactca gcctgtatga atctcagctc tatgcgtggc
     3301 gcagcaaact gcagcaacag atgacctcgt ctgagcgtga aagtgaactg cccgcctgaa
     3361 gcggcaactg gcggagcagg cagaggaact ggctatcctc caaaaggccg cgacatactt
     3421 tgcgcagcgg ctggtatacg tggtgtcagc gtcggacagg aataagccct cgtcagcagt
     3481 tccgtcagca ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt
     3541 acggtgcacc acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa
     3601 ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg
//
LOCUS       DBBBSB010000236         3587 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.242, whole genome shotgun sequence.
ACCESSION   DBBBSB010000236 DBBBSB010000000
VERSION     DBBBSB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.242"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="ACIUS0_004197"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="ACIUS0_004197"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGB0221688.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="ACIUS0_004198"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="ACIUS0_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGB0221689.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3130..3273)
                     /locus_tag="ACIUS0_004199"
     CDS             complement(3130..3273)
                     /locus_tag="ACIUS0_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221690.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3456..>3587
                     /locus_tag="ACIUS0_004200"
     CDS             3456..>3587
                     /locus_tag="ACIUS0_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGB0221691.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          894 a          859 c          941 g          893 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacaatatgt
      181 taacgaaatt aggtatggcg ggtgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agaaaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat aacaaaatgg
      541 cgattgttga taagcacgtg actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc cttgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttctgct gccgcatgtt caggtattca acagcaaagt cgccgctgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct
     1141 gtggctttac taacccgatc caggcaactc acgaagaaat tgtggtgatt tatcgcgccg
     1201 caatgtaatc atcattccaa caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaatcctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccatca agttaaaagc
     1441 ccgctacgac aactttattg gcggtgaatg ggtagcccct gccgatggtg agtattacca
     1501 gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgc gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct
     1681 gttagcgaca gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga
     1741 tgtaccgctg gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg
     1801 tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt
     1861 ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 tggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgcaacgc aaaacattat
     2161 tccggtgacg ctggagttgg gcggtaagtc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggcgaagtt tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg
     2701 caacggtaat ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa
     2761 ctgttatcac gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga accaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat
     3241 ctgtactgtg gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc
     3301 aatatgataa acctcgtcct gttgaaaaac cagacagcac atttcagaaa gtatataaaa
     3361 aacaatatgt cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc
     3421 gttaattgtt cttttttata ttctttgagg gaaatatgtt cctggactat tttgcactgg
     3481 gagtgcttat ttttgtattt ctggtgatat tctatgggat tattatttta catgatattc
     3541 cctacctgat tgctaaaaaa cgtaatcatc ctcatgccga cgccatt
//
LOCUS       DBBBSB010000237         3548 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.243, whole genome shotgun sequence.
ACCESSION   DBBBSB010000237 DBBBSB010000000
VERSION     DBBBSB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.243"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACIUS0_004201"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACIUS0_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGB0221692.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACIUS0_004202"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACIUS0_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGB0221693.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACIUS0_004203"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACIUS0_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGB0221694.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DBBBSB010000238         3496 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.244, whole genome shotgun sequence.
ACCESSION   DBBBSB010000238 DBBBSB010000000
VERSION     DBBBSB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.244"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..311
                     /locus_tag="ACIUS0_004204"
     CDS             <1..311
                     /locus_tag="ACIUS0_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGB0221695.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACIUS0_004205"
     CDS             468..1217
                     /locus_tag="ACIUS0_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGB0221696.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACIUS0_004206"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACIUS0_004206"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGB0221697.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACIUS0_004207"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACIUS0_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGB0221698.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3496
                     /locus_tag="ACIUS0_004208"
     CDS             3361..>3496
                     /locus_tag="ACIUS0_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGB0221699.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          961 a          908 c          864 g          763 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtt
//
LOCUS       DBBBSB010000239         3489 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.245, whole genome shotgun sequence.
ACCESSION   DBBBSB010000239 DBBBSB010000000
VERSION     DBBBSB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.245"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..77
                     /locus_tag="ACIUS0_004209"
     CDS             <1..77
                     /locus_tag="ACIUS0_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221700.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACIUS0_004210"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACIUS0_004210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGB0221701.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACIUS0_004211"
     CDS             1490..2614
                     /locus_tag="ACIUS0_004211"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGB0221702.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACIUS0_004212"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACIUS0_004212"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGB0221703.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          920 a          760 c          850 g          959 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac atcatccttt
     3481 ttgagtata
//
LOCUS       DBBBSB010000240         3476 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.246, whole genome shotgun sequence.
ACCESSION   DBBBSB010000240 DBBBSB010000000
VERSION     DBBBSB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.246"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..799
                     /gene="yibB"
                     /locus_tag="ACIUS0_004213"
     CDS             <1..799
                     /gene="yibB"
                     /locus_tag="ACIUS0_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGB0221704.1"
                     /translation="ANKGFREKLARSVDVYFSYFERLAALENEMIIFTSPDLKPRIEA
                     IRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCN
                     LKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDENKMHLFTIKKGL
                     TVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIF
                     VMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            1074..2270
                     /gene="kbl"
                     /locus_tag="ACIUS0_004214"
     CDS             1074..2270
                     /gene="kbl"
                     /locus_tag="ACIUS0_004214"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGB0221705.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2280..3305
                     /gene="tdh"
                     /locus_tag="ACIUS0_004215"
     CDS             2280..3305
                     /gene="tdh"
                     /locus_tag="ACIUS0_004215"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGB0221706.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          876 a          753 c          901 g          946 t
ORIGIN      
        1 tgctaataaa gggttccgtg aaaaactagc tcgttcagtt gatgtttatt ttagttactt
       61 tgagcgtctg gccgcacttg aaaatgagat gattattttc acttctcccg atttgaaacc
      121 aaggattgaa gcgattcgca acggaaaacc aacaacggtt atcgttatcg atataaaaaa
      181 gaaatttaga tatatcagaa gtcgtatcga aaaaattcaa aaagatgagt catttacaaa
      241 tagacttgag cctcgacaat taaaaaaccc agagtactgg tcaccagagt atgttttggt
      301 atgtaacctt aaggcctatt ttgtaaataa agctatcaac atgggtttag tgaaaacgcc
      361 tcttgttgcg tggatagatt ttggctattg ccgtaagcca aatgtaactc gaggattgaa
      421 aatttgggat ttcccatttg atgaaaataa gatgcatctt ttcactataa aaaaagggct
      481 tactgttaca tctcaacaac aggcttttga tttcatgatt ggcaatcatg tatatattat
      541 tgggggcgca attgttggtt cacagcataa atggaaagaa ttctataaat tagttctgga
      601 aagtcaaaaa ataacactaa acaataacat tgtggacgat gaccagggca tttttgtcat
      661 gtgctattac aaacgttccg atctcttcaa cctaaactat ctggggagag gaaaatggtt
      721 cgatttgttt cgctgcttca ggagtaacac tttaggggca aaaatgcagg cgctgagaat
      781 tttcctttca agaaaatagg tttccctcca ttagtgggta gcgttttgat gccaactgag
      841 ataggaaaaa tgatgctttg gcatcatttt ttctttttat atcaatgcag taaaatcact
      901 catcattatg ctcagccaaa actgtacagt ttttctacta tctgtaggtg ttgagagaca
      961 tcaagataca cgttatttat cctgaatttt gcagaagtgt taacgcatca tctcgtcgcg
     1021 acctataagt ttgggtaata tgtgctggaa tttgccctgt ctggagaatc gcaatgcgtg
     1081 gagaatttta tcagcagtta accaacgatc tggaaaccgc acgggcggaa gggttgttta
     1141 aagaagagcg cattattacg tctgcgcagc aagcagatat caccgtggct gatggaagcc
     1201 acgtcattaa cttttgtgcc aacaactatc tcgggctggc gaatcatccg gatctgattg
     1261 cggcagcaaa ggcgggaatg gattctcacg gtttcggcat ggcttcggtg cgttttattt
     1321 gcggcactca ggacagccat aaagagcttg aacaaaaact ggcggcattc ctggggatgg
     1381 aagatgcgat tctctactct tcctgctttg atgctaacgg tggcctgttt gaaacgctgc
     1441 tgggcgcgga agacgccatt atctccgacg cactgaacca cgcgtctatt attgatggtg
     1501 tgcgtctgtg taaagctaaa cgctatcgct atgccaacaa cgatatgcag gagcttgaag
     1561 ctcgtctgaa agaagcgcgt gaagccggtg cgcgtcatgt gctgattgcc accgatggtg
     1621 tgttctcgat ggacggcgtg attgccaatc tgaagggcgt ttgcgatctg gcagataaat
     1681 acgatgccct ggtgatggta gacgactccc acgcggtcgg ttttgtcggt gaaaatggtc
     1741 gtggttccca tgaatactgc gatgtgatgg ggcgtgtcga catcatcacc ggcacgctcg
     1801 gtaaagcgct gggcggggct tctggtggtt ataccgcggc gcgcaaagaa gtggttgagt
     1861 ggctgcgcca gcgttctcgt ccgtacctgt tctccaactc gctggcacca gcaattgtcg
     1921 ccgcttccat caaagtgctg gagatggttg aagcgggtag cgagttgcgt gaccgtctgt
     1981 gggcgaacgc gcgtcagttc cgtgagcaaa tgtcggcggc gggctttacc ctggcgggag
     2041 ccgatcacgc cattattccg gtcatgcttg gtgatgcggt agtggcgcag aaatttgccc
     2101 gagagctgca aaaagagggc atttacgtca ccggtttctt ctatccggtc gttccgaaag
     2161 gtcaggcgcg tattcgtacc cagatgtctg cggcgcatac ccctgagcaa attacgcgtg
     2221 cagtagaagc attcacgcgt attggtaaac aactgggcgt tatcgcctga ggatgtgaga
     2281 tgaaagcgtt atccaaactg aaagcggaag agggcatctg gatgaccgac gtccctgtac
     2341 cggaactcgg gcataacgat ctgctgatta aaatccgtaa aacagccatc tgcgggactg
     2401 acgttcacat ctataactgg gatgagtggt cgcaaaaaac catcccggtg ccgatggtcg
     2461 tgggccatga atatgtcggt gaagtggtag gtattggtca ggaagtgaaa ggcttcaaaa
     2521 tcggcgatcg cgtttctggc gaaggccata tcacctgcgg tcactgtcgc aactgtcgtg
     2581 gcggtcgtac ccatctgtgc cgcaacacca ttggtgtcgg tgttaaccgt ccgggctgct
     2641 tcgccgaata tctggtgatt ccggcgttca acgctttcaa aatccccgac aatatctccg
     2701 acgacctggc ttccattttt gatcccttcg gtaacgccgt gcataccgcg ctgtcgttcg
     2761 atctggtggg cgaagatgtg ctggtttctg gtgcaggtcc gataggtatt atggctgcgg
     2821 cggtggcgaa acacgttggt gcacgcaatg tggtgatcac cgatgttaac gaataccgcc
     2881 ttgagctggc gcgcaaaatg ggtatcaccc gtgcggttaa cgtcgcgaaa gaaaacctta
     2941 atgatgtgat ggctgaactg ggcatgaccg aaggctttga tgtcggtctg gaaatgtccg
     3001 gcgcgccgcc agcgtttcgt accatgctcg ataccatgaa ccacggcggt cgtattgcga
     3061 tgctgggtat tccaccgtct gatatgtcta tcgactggac caaagtgatc tttaaaggct
     3121 tgttcattaa aggcatttac ggtcgtgaga tgtttgaaac ctggtacaag atggcggcgc
     3181 tgattcagtc tggcctcgat ctctcgccga tcattaccca tcgtttctct atcgatgatt
     3241 tccagaaggg ctttgacgct atgcgttcgg gccagtccgg gaaagttatt ctgagctggg
     3301 attaacacga acaagggctg gtattccagc ccttttatct gaggataatc tgttaattat
     3361 gtaaaatcct gtcagtgtaa taaagagttc gtaattgcgc tgatctttta tatagctgtt
     3421 ctcattatct ctctaccctg aagtgactct ctcacctgta aaaataatat ctcaca
//
LOCUS       DBBBSB010000241         3456 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.247, whole genome shotgun sequence.
ACCESSION   DBBBSB010000241 DBBBSB010000000
VERSION     DBBBSB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.247"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(226..429)
                     /locus_tag="ACIUS0_004216"
     CDS             complement(226..429)
                     /locus_tag="ACIUS0_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221707.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACIUS0_004217"
     CDS             complement(431..586)
                     /locus_tag="ACIUS0_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGB0221708.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACIUS0_004218"
     CDS             complement(789..1196)
                     /locus_tag="ACIUS0_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221709.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACIUS0_004219"
     CDS             1273..1500
                     /locus_tag="ACIUS0_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGB0221710.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACIUS0_004220"
     CDS             1484..1906
                     /locus_tag="ACIUS0_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGB0221711.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACIUS0_004221"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACIUS0_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACIUS0_004222"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACIUS0_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DBBBSB010000242         3430 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.248, whole genome shotgun sequence.
ACCESSION   DBBBSB010000242 DBBBSB010000000
VERSION     DBBBSB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.248"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            277..612
                     /locus_tag="ACIUS0_004223"
     CDS             277..612
                     /locus_tag="ACIUS0_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGB0221712.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            770..1213
                     /gene="yjdN"
                     /locus_tag="ACIUS0_004224"
     CDS             770..1213
                     /gene="yjdN"
                     /locus_tag="ACIUS0_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGB0221713.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1346..2134
                     /gene="phnC"
                     /locus_tag="ACIUS0_004225"
     CDS             1346..2134
                     /gene="phnC"
                     /locus_tag="ACIUS0_004225"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGB0221714.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            2159..3175
                     /gene="phnD"
                     /locus_tag="ACIUS0_004226"
     CDS             2159..3175
                     /gene="phnD"
                     /locus_tag="ACIUS0_004226"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0221715.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3281..>3430
                     /locus_tag="ACIUS0_004227"
     CDS             3281..>3430
                     /locus_tag="ACIUS0_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HGB0221716.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT          891 a          943 c          866 g          730 t
ORIGIN      
        1 aataaataac gtcttctgat gcgtttaaaa cgtcagaaag ataaaaatat catgtgaata
       61 aaaaaaagaa caagtagagc attaacatta tcttaaataa taaatagagg caaaaagatt
      121 attttctttt tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc
      181 ccgacaaact cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt
      241 ggcgcttttg ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa
      301 tgcaactccg aatacactta cgaagataac ggcatgtaca tctgcccgga atgtgcctac
      361 gaatggaacg acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac
      421 ggcaatctgc tggctgacgg cgacagcgtt accatcatta aagatctgaa ggtgaaaggt
      481 agctcttcga tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac
      541 cataacatcg attgcaaaat cgacggtttt ggtccgatga aactgaaatc tgagtttgtg
      601 aaaaagaact gattgtattg tgatcggtaa gccggataag gcgctcgcgc cgcatccggc
      661 aacgatgccg actgcctgat gcgacgcttg cgcgtcttat caggcagcct ctcatctgta
      721 taaatttcga actacactta actggcttct cttaactgag gtcaccatca tgccgttaag
      781 tccctacctc tcttttgccg gtaactgttc cgacgcgatt gcctattatc aacgtacgtt
      841 gggcgcggaa ctgctctata aaatcagctt cggcgaaatg ccgaaatcag cgcaggacag
      901 cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgctc atgccaacgt
      961 gcgcattgcc ggaagcgaca tcatgatgag cgatgctata ccgtcaggaa aagccagcta
     1021 ctccggcttt acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga
     1081 taaccttgcc gctaacggaa aaatcgaaat ggcctggcag gaaactttct gggcgcatgg
     1141 ctttggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca
     1201 accaacgcaa taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa
     1261 ctcttcatct gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga
     1321 gccacttttt tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa
     1381 aaccttcaat cagcatcaag cgctgcatgc ggttgatctg aacattcatc acagtgaaat
     1441 ggtggctctg cttgggccgt cgggttccgg caaatccacc cttttacgtc atttaagcgg
     1501 tttgattacc ggcgataaat ccgccggtag ccatatcgag ctactgggcc gcactgtcca
     1561 gcgcgaaggc cgcctggccc gcgatatccg caaaagccgc gccaacaccg gctacatctt
     1621 tcaacaattc aacctggtga accgcctgag cgtactggag aacgtgctga ttggcgcgct
     1681 cggcagcacg ccgttctggc gcacctgttt tagctggttc accggcgagc agaaacagcg
     1741 cgcgttacag gcgctgaccc gcgttggcat ggtgcatttt gcccatcagc gcgtttccac
     1801 cctctccggc ggccagcagc aacgtgtggc gattgcccgt gcgctgatgc agcaggcgaa
     1861 agtgattctg gccgatgaac ccatcgcctc gctggacccg gaatcagcgc gcatcgtgat
     1921 ggacaccctg cgcgacatca accagaacga cggcatcacc gtggtcgtca cgctgcatca
     1981 ggtggattac gccctgcgct actgcgaacg catcgtcgcc ctgcgtcagg ggcacgtctt
     2041 ctacgacggc agcagccaac tgtttgataa cgaacgtttt gaccatctct accgcagcat
     2101 taaccgcatc gaagagaacg cgaaagctgc ctgatatccc catcattgag gaaaacgaat
     2161 gaacgctaag ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt
     2221 aaccccggca cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga
     2281 atcacagcaa aacctgaaac cgcaatggac gccgttttta caggatatgg agaagaaact
     2341 gggcgtgaag gtcaacgcct tctttgcccc ggactacgcg ggcattatcc aggggatgcg
     2401 cttcaataaa gtggatattg cctggtacgg caacctgtcg gcgatggaag cggtggatcg
     2461 cgccaatggc caggtcttcg cccagacggt cgcggcggat ggatcgccgg gttactggag
     2521 cgtgttgatc gtcaacaaag acagcccgat caacaacctg aacgatctgc tggcgaagcg
     2581 gaaagatctc accttcggca atggcgatcc taactccacc tctggcttcc tcgtccccgg
     2641 ttactacgtc ttcgctaaaa acaatatctc cgccagcgac ttcaagcgca ccgtcaacgc
     2701 cgggcatgaa accaacgcgc tggccgtcgc caacaagcaa gtggatgttg ccaccaacaa
     2761 caccgaaaac ctcgacaagc tgaaaacctc cgcgccggag aagcttaaag agctgaaggt
     2821 gatctggaaa tcgccgctga tcctaggcga tccgatcgtc tggcgcaaga atctgtccga
     2881 aactaccaaa gacaagatct acgacttctt tatgaactac ggaaaaacgc cggaagagaa
     2941 agcggtgctg gaacgcctgg gctgggcgcc gttccgcgct tccagcgacc tgcaactggt
     3001 gccgattcgc cagctcgcac tgtttaaaga gatacaaagc gtgaaaggca ataaagggct
     3061 gaatgagcag gacaagctgg cgaaaaccac cgagattcag gcgcagctgg atgacctgga
     3121 ccgcctgaac aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc agtaaatcgt
     3181 aggtcggata agacgccccg gcgtcgcatc cgacaatgtg taggcgttga tgccggatgc
     3241 ggtgcaagca ccttatccgg cctacagacc ggagccaaac atgcaaacca tcaccatcgc
     3301 cccacccaag cgtagctggt tctcgcttct gagctgggcc gttgttctcg acgtgctggt
     3361 cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg attaaagacg gcggcaacat
     3421 ggcaaccttc
//
LOCUS       DBBBSB010000243         3425 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.249, whole genome shotgun sequence.
ACCESSION   DBBBSB010000243 DBBBSB010000000
VERSION     DBBBSB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.249"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..328
                     /locus_tag="ACIUS0_004228"
     CDS             <1..328
                     /locus_tag="ACIUS0_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0221717.1"
                     /translation="DHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVP
                     SKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAF
                     KTHFHI"
     gene            complement(306..929)
                     /gene="tetR(B)"
                     /locus_tag="ACIUS0_004229"
     CDS             complement(306..929)
                     /gene="tetR(B)"
                     /locus_tag="ACIUS0_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HGB0221718.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1011..2216
                     /gene="tet(B)"
                     /locus_tag="ACIUS0_004230"
     CDS             1011..2216
                     /gene="tet(B)"
                     /locus_tag="ACIUS0_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HGB0221719.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2329..2922)
                     /gene="tetC"
                     /locus_tag="ACIUS0_004231"
     CDS             complement(2329..2922)
                     /gene="tetC"
                     /locus_tag="ACIUS0_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HGB0221720.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3010..>3425
                     /locus_tag="ACIUS0_004232"
     CDS             3010..>3425
                     /locus_tag="ACIUS0_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221721.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
BASE COUNT          929 a          624 c          703 g         1169 t
ORIGIN      
        1 tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc aacagcaatg
       61 gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc atgaaatggg
      121 aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag attggagtga
      181 acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt atgaatctaa
      241 agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa aaggttatgc
      301 tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat gatcaattca
      361 aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtcgt
      421 aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc gacttgatgc
      481 tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag tgcatataat
      541 gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc gagagtttca
      601 tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg atgacttagt
      661 aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct ttccccttct
      721 aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa
      781 gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag cttctgggcg
      841 agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa tgcgctgtta
      901 atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt gacactctat
      961 cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa atgaatagtt
     1021 cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc cttatcatgc
     1081 cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct aaccactttg
     1141 gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg cttggaaaaa
     1201 tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc gcatcgctgg
     1261 attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt
     1321 cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat accacctcag
     1381 cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt ggtttaatag
     1441 cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc ttttttatcg
     1501 ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt gaaaccaaaa
     1561 atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat tcggtataca
     1621 tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg caattgatag
     1681 gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga tggaatagca
     1741 tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc caagcctttg
     1801 tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc ggatttattg
     1861 cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta gttttccctg
     1921 ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga gtgatgtcta
     1981 tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc cttaccaatg
     2041 caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca ctaccaattt
     2101 gggatggctg gatttggatt attggtttag cgttttactg tattattatc ctgctatcga
     2161 tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt gcttagttat
     2221 ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa cccaagaggg
     2281 cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt atttatcttt
     2341 caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat cggcaagctc
     2401 ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta agtttttaag
     2461 cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga caatggcgtt
     2521 taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac caatgatagg
     2581 catcacttct tggataggga taaggttttt attattatca ataatataat caagataatg
     2641 ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg ttgtgatgta
     2701 ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt ttttattttt
     2761 aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct catctattga
     2821 aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca gtgattgata
     2881 ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt taaaataaca
     2941 taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta agaacattta
     3001 ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa taacatccaa
     3061 ttaagatatg atgataagca gtttcataca acggttatca aagatgttct attatggatt
     3121 gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc gggttatacc
     3181 aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc tagctatatt
     3241 cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa aactatcctt
     3301 gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg atttaagtac
     3361 atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt ggaggcaatg
     3421 cactg
//
LOCUS       DBBBSB010000244         3418 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.250, whole genome shotgun sequence.
ACCESSION   DBBBSB010000244 DBBBSB010000000
VERSION     DBBBSB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.250"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..239
                     /locus_tag="ACIUS0_004233"
     CDS             <1..239
                     /locus_tag="ACIUS0_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGB0221722.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            249..887
                     /locus_tag="ACIUS0_004234"
     CDS             249..887
                     /locus_tag="ACIUS0_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGB0221723.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            890..2053
                     /locus_tag="ACIUS0_004235"
     CDS             890..2053
                     /locus_tag="ACIUS0_004235"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGB0221724.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2137..>3418
                     /locus_tag="ACIUS0_004236"
     CDS             2137..>3418
                     /locus_tag="ACIUS0_004236"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGB0221725.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGS"
BASE COUNT          760 a          806 c          988 g          864 t
ORIGIN      
        1 cattgttagg ttacgttatc ccattatcca gtgcggcaat caatgcggtg agtattccag
       61 actatttcgc ctggggcggg attgcactgg tgattcagtt actcgttttt gctggtgtca
      121 gactttatat gcccgcatta agcgaaaaaa ttattaatca caataccgca gcaggaatgt
      181 ttatgggaac cgccgcgctg gctggcggta tttttaacgc agcttgtatg acatggtaat
      241 ggacgatcat ggccagaaaa cgcaaatcaa gaaataacag taaaattggt cacggagcga
      301 tcagtcgcat tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa
      361 aatacttaac gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtagcg
      421 atagtagcga tgtcgataac gacggcgacg gaacatttta cgcgacagtg caggattgta
      481 ttgatgacgg taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaagcggcat
      541 tttatgccga tgttccaaag aatatgactc agcagaactg tcagtctaag tacgaaaatt
      601 gctactacga caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc
      661 gggttattcg taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg
      721 cttcccgccc cgtctggcgc aacacttccg gtgattactc ctggcgcttc ggttctggca
      781 aaaaagagtc ttactcttca ggcggcttta ccaccagaaa agcgtcgacc gtttcgcgcg
      841 gcggctatgg tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca
      901 caacgttcct gtgcggcggg atctggacca gatcgccgct aacaacggtt tcgactttca
      961 tatcatcgac aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca
     1021 gattgaagag cagatcgaaa aaccgactgc ggaactgcat cagatgtgcc ttgaggtggt
     1081 ggatcgcgcg gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg
     1141 ggatgtgatc gccgaaagct ggcgcgcacg cgatccttcg ctgtatggcc ggatggattt
     1201 tgcctggtgt ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacctc
     1261 gttgtatgag tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat
     1321 tattccgcgt gatgccgatc agtacaacgc cattcaggaa cgcctgattt cgcgctttag
     1381 tgagctttac agtcgggaac tgttttattt ttgctgctgt caggacaccg atgaagacag
     1441 gagtaccgtg ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat
     1501 ctacattgaa gatctcggtt tgggcgtcgg cggcgtactg accgatcttg atgataatgt
     1561 catccagcgt gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc
     1621 gctgctgcgc aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa
     1681 taaagggcta atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc
     1741 ctggtttgag ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat
     1801 ctactcgcgc gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca
     1861 cgctgatggt gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg
     1921 atttggcgat agctacacac tcatcggtag ctggattgtc gatgatgaag cgtgcggaat
     1981 ggggatccgt gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta
     2041 cattgctgga taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtgacca
     2101 tactaatggt gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccatt
     2161 tttaatcaac ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc
     2221 gaagcggtaa cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag
     2281 cagttaggaa cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa
     2341 ctgttgcgct acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg
     2401 cacctgctga tgcaggcgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac
     2461 gctcgctccg gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag
     2521 gctgggtcgt tatgtccagt aacgatgacc tttgccgcca cgccattgtt gttacagatg
     2581 ttacccacgc cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac
     2641 ttattgccag gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag
     2701 ggcggttccg atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat
     2761 cggctggtgg ggcataaatg gtttttctcg gtgccgcaaa gcgatgcgca tctggtgctg
     2821 gcgcagaccg cgggcggtct gtcctgcttt tttgtgccgc gctttttgcc tgacgggcaa
     2881 cgcaacgcga ttcgcctcga gcggctgaaa gataagctgg gtaatcgctc taacgccagt
     2941 tgcgaagtgg agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt
     3001 cgtctgatcc tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc
     3061 atgatgcgcc gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt
     3121 aatccattga tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt
     3181 gaagggcaaa cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc
     3241 aaagaagccc tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga
     3301 ggtatgccgt ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag
     3361 agcgagctgc cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccg
//
LOCUS       DBBBSB010000245         3405 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.251, whole genome shotgun sequence.
ACCESSION   DBBBSB010000245 DBBBSB010000000
VERSION     DBBBSB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.251"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..98
                     /locus_tag="ACIUS0_004237"
     CDS             <1..98
                     /locus_tag="ACIUS0_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGB0221726.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACIUS0_004238"
     CDS             156..512
                     /locus_tag="ACIUS0_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221727.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACIUS0_004239"
     CDS             605..823
                     /locus_tag="ACIUS0_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGB0221728.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACIUS0_004240"
     CDS             825..1190
                     /locus_tag="ACIUS0_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGB0221729.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACIUS0_004241"
     CDS             1187..1852
                     /locus_tag="ACIUS0_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221730.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACIUS0_004242"
     CDS             1852..2217
                     /locus_tag="ACIUS0_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGB0221731.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..2827
                     /gene="hokD"
                     /locus_tag="ACIUS0_004243"
     CDS             2672..2827
                     /gene="hokD"
                     /locus_tag="ACIUS0_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGB0221732.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3103..3315
                     /locus_tag="ACIUS0_004244"
     CDS             3103..3315
                     /locus_tag="ACIUS0_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221733.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
     gene            complement(3333..>3405)
                     /locus_tag="ACIUS0_004245"
     CDS             complement(3333..>3405)
                     /locus_tag="ACIUS0_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221734.1"
                     /translation="SFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          983 a          693 c          874 g          855 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga
     2821 ggagtaagag accaggcggg ggagaaatcc ctcgccacct ctcatgtgtc aggcatcctc
     2881 aacgcacccg cactaaaccc gcttcggcgg gttttttgtt gcgtgctgaa tacgcagggt
     2941 gaaaaataac catatatttg attatataca caacaaaaaa taaaagtcat tgtacttgca
     3001 cattaaacaa tcaaatatac ggcgtgaaat aaatatttat cagattaata tttttgtctc
     3061 tatgtggata taaccgtttg tacttataaa cctgggggca tcgtggaaaa aataaagaaa
     3121 ctttttagta gcaaatacgc agtcatacgt cgtgatgacc tgtcagttat agtcgaaatg
     3181 gattacttcc ctgaaacccc aaaatcaata atgtatcgta atggtcgaaa ggcaattttt
     3241 ttaccgatga gggtaagtga cattatggga aatgataaac tgctggatga attgcgagtc
     3301 agagcatcct gttagtaggt aatgactcca acttactgat agtgttttat gttcagataa
     3361 tgcccgatga ccttgtcatg cagctccacc gattttgaga acgac
//
LOCUS       DBBBSB010000246         3348 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.252, whole genome shotgun sequence.
ACCESSION   DBBBSB010000246 DBBBSB010000000
VERSION     DBBBSB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.252"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            19..897
                     /locus_tag="ACIUS0_004246"
     CDS             19..897
                     /locus_tag="ACIUS0_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGB0221735.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACIUS0_004247"
     CDS             894..2285
                     /locus_tag="ACIUS0_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGB0221736.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACIUS0_004248"
     CDS             2282..2539
                     /locus_tag="ACIUS0_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221737.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACIUS0_004249"
     CDS             2634..2834
                     /locus_tag="ACIUS0_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HGB0221738.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACIUS0_004250"
     CDS             2827..3207
                     /locus_tag="ACIUS0_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGB0221739.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DBBBSB010000247         3334 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.253, whole genome shotgun sequence.
ACCESSION   DBBBSB010000247 DBBBSB010000000
VERSION     DBBBSB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.253"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            95..2248
                     /locus_tag="ACIUS0_004251"
     CDS             95..2248
                     /locus_tag="ACIUS0_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HGB0221740.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>3334
                     /locus_tag="ACIUS0_004252"
     CDS             2301..>3334
                     /locus_tag="ACIUS0_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGB0221741.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYA"
BASE COUNT         1210 a          597 c          609 g          918 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttg
//
LOCUS       DBBBSB010000248         3328 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.254, whole genome shotgun sequence.
ACCESSION   DBBBSB010000248 DBBBSB010000000
VERSION     DBBBSB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.254"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..209
                     /locus_tag="ACIUS0_004253"
     CDS             <1..209
                     /locus_tag="ACIUS0_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221742.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            270..458
                     /locus_tag="ACIUS0_004254"
     CDS             270..458
                     /locus_tag="ACIUS0_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221743.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            455..1282
                     /locus_tag="ACIUS0_004255"
     CDS             455..1282
                     /locus_tag="ACIUS0_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGB0221744.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1326..2075
                     /locus_tag="ACIUS0_004256"
     CDS             1326..2075
                     /locus_tag="ACIUS0_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGB0221745.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2072..2641
                     /locus_tag="ACIUS0_004257"
     CDS             2072..2641
                     /locus_tag="ACIUS0_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221746.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2638..2760
                     /locus_tag="ACIUS0_004258"
     CDS             2638..2760
                     /locus_tag="ACIUS0_004258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221747.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2765..3007
                     /locus_tag="ACIUS0_004259"
     CDS             2765..3007
                     /locus_tag="ACIUS0_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGB0221748.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3011..3157
                     /locus_tag="ACIUS0_004260"
     CDS             3011..3157
                     /locus_tag="ACIUS0_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221749.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3166..>3328
                     /gene="xisR"
                     /locus_tag="ACIUS0_004261"
     CDS             3166..>3328
                     /gene="xisR"
                     /locus_tag="ACIUS0_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGB0221750.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          929 a          797 c          854 g          748 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca
      421 tttataaggg tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc
      481 ccttgttctg ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg
      541 catcaaggta aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg
      601 ctactggacg ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac
      661 ctacgatgag ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg
      721 cgtggcctac aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg
      781 cgtggacgac attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact
      841 ggccagcaaa atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc
      901 agctcttaaa gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc
      961 atatgtgggt aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac
     1021 ggcgaaccag aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa
     1081 catgttgcgc gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga
     1141 agccgatgct attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct
     1201 ggaagccatc aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac
     1261 accatttatt caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga
     1321 taaaaatgaa tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc
     1381 agcgtgttgt gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc
     1441 gtgcagcatt caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag
     1501 gtgcggacgt aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg
     1561 ggattgtact ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg
     1621 cctggcaggt tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg
     1681 aaatcgagat gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc
     1741 atgtggaaga acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata
     1801 tgcgcgccgg atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca
     1861 agtgtcggaa tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc
     1921 cggacggtct gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc
     1981 accaaatgat gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc
     2041 ttttccaggt gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc
     2101 cgctctggtg tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac
     2161 gccgaaacat attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg
     2221 gacgaagaaa aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac
     2281 agagctgatt tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact
     2341 gtccattacc ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc
     2401 gaaaaagttt gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga
     2461 gaagatgttt ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg
     2521 gcaagcggtc agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt
     2581 tgttgtgatg ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg
     2641 aaaatcgaat accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta
     2701 actctgggga tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa
     2761 ccacatgttc gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt
     2821 agaaattact cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg
     2881 gcgagtacgc atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca
     2941 caagattctg gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag
     3001 gaagtaaaaa atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta
     3061 cgacctgacg cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga
     3121 ggtctggtgc cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt
     3181 caactggctc ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa
     3241 ccaggcacaa ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac
     3301 gtatcaccag acggtaatcc aaagcctt
//
LOCUS       DBBBSB010000249         3255 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.255, whole genome shotgun sequence.
ACCESSION   DBBBSB010000249 DBBBSB010000000
VERSION     DBBBSB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.255"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            55..390
                     /locus_tag="ACIUS0_004262"
     CDS             55..390
                     /locus_tag="ACIUS0_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGB0221751.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(671..779)
                     /locus_tag="ACIUS0_004263"
                     /pseudo
     CDS             complement(671..779)
                     /locus_tag="ACIUS0_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1302..1378)
                     /locus_tag="ACIUS0_004264"
     tRNA            complement(1302..1378)
                     /locus_tag="ACIUS0_004264"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1342..1344),aa:Arg,seq:tct)
     gene            complement(1480..1555)
                     /locus_tag="ACIUS0_004265"
     tRNA            complement(1480..1555)
                     /locus_tag="ACIUS0_004265"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1520..1522),aa:Ile,seq:cat)
     gene            complement(1700..2521)
                     /locus_tag="ACIUS0_004266"
     CDS             complement(1700..2521)
                     /locus_tag="ACIUS0_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGB0221752.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(2536..2892)
                     /locus_tag="ACIUS0_004267"
     CDS             complement(2536..2892)
                     /locus_tag="ACIUS0_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGB0221753.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(2905..>3255)
                     /locus_tag="ACIUS0_004268"
     CDS             complement(2905..>3255)
                     /locus_tag="ACIUS0_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGB0221754.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          907 a          882 c          635 g          831 t
ORIGIN      
        1 atatcattta tacttttcag aacatcacca gcaaggcata aacaaggaaa ctaaatgaag
       61 tggattgtga ttgatacagt tatccagcca tcatgcggaa tatctttttc agtcatatgg
      121 agtaaaataa aattaataat ctggtatcaa tcggatgctt tcttacctcc tgaaagtata
      181 tttacactga ctcacacagg catcatgctc aataacaaag tgctacctgt aaccatttac
      241 aacgtagtac cattcaataa aacattctgg aatttaatca aaaacagcca ggaatgccct
      301 acaaatacag ataacgtatt gaatgaatgc tttaataacc gttgcactct gcaaatatgc
      361 ccttatggac taaaacaaca aagtccataa ggagtttact cacatctgac aaaatcaata
      421 taaacagccc ctccggagag gggctggaga gtggcgctat gtgccattcc atggtgccgg
      481 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      541 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      601 caaaccagac aacagtcaac cgcctgaatt gtgaagtatt taaaaatttc tcccgctaac
      661 tgatacccgg ctaacagtct ggcgttttct ttttcagcaa cgggaaagca acaaccacca
      721 cacccgccac cagcacaccg tcagccggca ctgacattat ccggctgctg caatgccatt
      781 aacaaaaaca gtaagcaatc actttttacc gtaacaggtg ataatccaga tatgtatcta
      841 ccccagatga gtaatccgaa gttcatccat accacaggtc ctggctattc tgttgtactc
      901 ctgaacaaga gcaaataatt ctgaattagc aaccatgaac tcatcgcaaa ccctctgtat
      961 agcatcacta ttcagaataa taacgtctct tcccgaaaga cgatcaggag tacagaacaa
     1021 aactgtcaaa cggctgaagg cctttgctcg tgctgcattg actatatcaa tacgctgcct
     1081 aaggatgaaa caccccgacg cctcatcaat attcactcta cccacaccat atgaatgata
     1141 aatacttaat gctgaaaaaa ccattagacc gtataacaaa tactcaatca atacttaaca
     1201 gaacttttat ttttgacaaa tatataatat tttcaacaat atcctgagcc aggtatattt
     1261 cagtataagg ctctgccgga aggaatctgg aagaatgaat atggcgcgct gtactggatt
     1321 cgaaccagtg accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgctg
     1381 aataccgata atggaccgcc atcggggacc cgccccccgc accaacaacc ctgttatcgt
     1441 gtcgtctgct cttcctgata agctaatggc ggtttgtgat ggtggccctt gctggatttg
     1501 aaccagcgac ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccgggag
     1561 cagaataata acggtccgta attaatttcg caataaaaaa aaccgctcag cggcgggttg
     1621 tagaaactct tctaacgcca ggcataaaac gcccatcatt atgacgaatt taccacagat
     1681 tccggaaaaa tcaaccttgt tacctagtta ccttttttaa ctgccgctca gcccatgctt
     1741 cttcaatatc aaaccgggtc accagcgcat cataaaattt cttaactgtt ttttcccatg
     1801 acgcgcgtgt tatctggttt gtcacctcgc atatagcatt aaatgcctcc gttgatggta
     1861 gtctttcata gccacgacca ccacaacgct ggcagtctct gataacaggc ataccacgtt
     1921 ttaccgactc ttcacgatga atggcgacac cacgcccacg gcaatcctta caggcggtgg
     1981 aaacctcacc ctttccgcca cactccggac aggcaacttt taccacctcc ctgacttttt
     2041 tccattcttc ccagtaaaac ggatacacac ctttcgtaca ctttgcccat accggcggct
     2101 taccatccgg atactggatc ttgtttgtaa aaactatgct ttcaataaat ttttccccat
     2161 agcaacaagg gcactgcttt ttactcgctg cgctgcgggc ataatcctca aaagcgtacg
     2221 aagccataat gcgcatcact accggtttta tttctgccgg aagttttctc aacgccgcca
     2281 cacgatcgca ccgactgagt gcataatctg ccagtaattc tgttgcccgc gccctgtcat
     2341 tcatactgat gcccattttc cccaggaacg cagaaaaacc catctcagcc cgattctgtg
     2401 tcatgccctg cgcggccatc acatcagtga tactcagcgc atcttttgac gttgaggccg
     2461 atgcatcggt caggccaggg gattttgggg agtagtattt cggtaaatct tccagtttca
     2521 ttttttgacc tgctcttaat gcattatggg gtaaatcttc acccccagac gtccaccaga
     2581 tactggctga ccacgaacga tattgatttc atcaaactgc tcatcgtcca ttaacactcc
     2641 cgcatgcgtc agcgcatcca gcggtgcttt caggatattg tccaggtcgc gacgacgctt
     2701 atccggtggc tctgcaatca cctttatcgc cagccttccg gacaggctta atttcagccg
     2761 ctgctggcga acaataagcg ccacagcccg gcgataacgc tttccctcct ccgagataaa
     2821 atatgtgctg ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gtaaaaccaa
     2881 atctatgagc atcagtcacc tcttttaccc aagcacgcca gttgcaaagg cgtgatcaag
     2941 aaaacgaaaa attaaatcaa cctgagaacc atgcttttct tcgaacgcca gaggatccgc
     3001 atgaagctcg ttgtgatgct cccgacacag cggtagcgtg aaaatatcgt gagatttggt
     3061 ccccattccg ccctgaccat gaccaatcag gtgatgggga tcgtcggctg gcttaccaca
     3121 acacgcacac ggctgtgtct tcacccagcg tttgtatttc tcgttaaccc agcggcgacg
     3181 tttaggtcgt ttcatgaaag attccggaga ctcaggatca acggcaatgc tgaccaccgt
     3241 cttttcctgt tgtgg
//
LOCUS       DBBBSB010000250         3227 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.256, whole genome shotgun sequence.
ACCESSION   DBBBSB010000250 DBBBSB010000000
VERSION     DBBBSB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.256"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACIUS0_004269"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACIUS0_004269"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGB0221755.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACIUS0_004270"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACIUS0_004270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGB0221756.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="ACIUS0_004271"
     tRNA            2652..2727
                     /locus_tag="ACIUS0_004271"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..3200)
                     /locus_tag="ACIUS0_004272"
                     /pseudo
     CDS             complement(2742..3200)
                     /locus_tag="ACIUS0_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          761 a          814 c          888 g          764 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct
     2761 tccttatttc ttgactggga caaatttggg accgatgggt tcagaatcaa gtctatttgc
     2821 cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     2881 gactcccagc ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa
     2941 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caataccagc ccgtctcagc
     3001 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc ttactgttgg cgctttcgtt
     3061 ccgtctggtt tggtacagct ttccttgtac acaaataccc aacggtgatg atttccgatt
     3121 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     3181 ttactctctt tgaggtgtac tggcaatagc ggacactacc atttgtt
//
LOCUS       DBBBSB010000251         3191 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.257, whole genome shotgun sequence.
ACCESSION   DBBBSB010000251 DBBBSB010000000
VERSION     DBBBSB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.257"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(29..655)
                     /gene="dsbA"
                     /locus_tag="ACIUS0_004273"
     CDS             complement(29..655)
                     /gene="dsbA"
                     /locus_tag="ACIUS0_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGB0221757.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(672..1658)
                     /gene="srkA"
                     /locus_tag="ACIUS0_004274"
     CDS             complement(672..1658)
                     /gene="srkA"
                     /locus_tag="ACIUS0_004274"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGB0221758.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1735..2004)
                     /locus_tag="ACIUS0_004275"
     CDS             complement(1735..2004)
                     /locus_tag="ACIUS0_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGB0221759.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2074..2658
                     /gene="mobA"
                     /locus_tag="ACIUS0_004276"
     CDS             2074..2658
                     /gene="mobA"
                     /locus_tag="ACIUS0_004276"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGB0221760.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2640..3167
                     /gene="mobB"
                     /locus_tag="ACIUS0_004277"
     CDS             2640..3167
                     /gene="mobB"
                     /locus_tag="ACIUS0_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGB0221761.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          837 a          774 c          766 g          814 t
ORIGIN      
        1 tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat ttcaccgtat
       61 cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc ggattcagct
      121 gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca gctgcggctt
      181 tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg tcgtactctt
      241 cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa cgaatggtct
      301 gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg cccagcgcca
      361 tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg aagttgacgt
      421 ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca gaaatatgca
      481 gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc agcacttgcg
      541 gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca tactgcgccg
      601 ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt ttcatgatct
      661 actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag aacttttgcc
      721 tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa ccacgggaaa
      781 tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata ataaaccaaa
      841 cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc gctaaattct
      901 tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc gccattgagc
      961 aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc aacaaacatt
     1021 ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg cagaacggtg
     1081 aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc tttcagaaat
     1141 gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg aggctcaatg
     1201 agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg ccccgtctgg
     1261 tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc agcttcgaac
     1321 tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt caataaagtc
     1381 tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag ctgcaacgca
     1441 aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata aaatttgacg
     1501 acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata gctgttaagc
     1561 ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat gatggtatcc
     1621 gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg aaaacattac
     1681 gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa gcttttactc
     1741 tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat aatctttctg
     1801 caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat agatcagaat
     1861 gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa
     1921 aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga gttcaataac
     1981 ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag acacgttagc
     2041 agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac gataacaggc
     2101 gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg attgctggac
     2161 ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca gctctctcac
     2221 gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct gaaagtgatt
     2281 gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt aatgcagcag
     2341 gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc ccatgattta
     2401 gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa
     2461 cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt actggaatat
     2521 ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg tcatgcggtt
     2581 gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga gcttgcccga
     2641 tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt accggaaaaa
     2701 ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt ccggggctga
     2761 ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc tatgagctgc
     2821 gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc ttgatgacgg
     2881 aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg gatacctcaa
     2941 agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag attgtgctgt
     3001 ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat gttattgctg
     3061 tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat gatgttgagg
     3121 ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca agagtaggct
     3181 ggatcagacg c
//
LOCUS       DBBBSB010000252         3146 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.258, whole genome shotgun sequence.
ACCESSION   DBBBSB010000252 DBBBSB010000000
VERSION     DBBBSB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.258"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            799..1908
                     /gene="wzz(fepE)"
                     /locus_tag="ACIUS0_004278"
     CDS             799..1908
                     /gene="wzz(fepE)"
                     /locus_tag="ACIUS0_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGB0221762.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2153..2287)
                     /locus_tag="ACIUS0_004279"
     CDS             complement(2153..2287)
                     /locus_tag="ACIUS0_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGB0221763.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2933..3103)
                     /locus_tag="ACIUS0_004280"
     CDS             complement(2933..3103)
                     /locus_tag="ACIUS0_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGB0221764.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          798 a          652 c          756 g          940 t
ORIGIN      
        1 tggtaattgt aagacgttgc gggcgacggt attacaattg cacatcctgt cctgtttctt
       61 taggacgtat catggcacag catatcaact gataaataat ccctgtgcag tgcagccgct
      121 gttgcataaa atttcgctct gcgtcgagtg tttcttaatc aataattccg ctgatactcc
      181 ggaaaaacgc gagtctgttc tcatgcctga aatgcccaca ccccacgcaa aaacaagttt
      241 ttgctgattt ttctttataa atagagagtt atgacaaatt agttcttctt gctctctttg
      301 tgatatttaa aaaagcggtg tcggcgcggt gttgtagctg cgccaacacc gctttttagg
      361 ggtggtactg actattttca taaaaaaaac attatcttat attaggggtg ctgctagcgg
      421 cgcggtgtgt ttttttacag gacgcccctg ggggcgctgc taggggtgtc tgttcagata
      481 tgtagcaatg agtcagggat tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac
      541 tgtatgcgcc caaaccgatc gtcattcact ttcatgcata gctatgcagt gagctgaaag
      601 cgatcctgac gcatttttcc ggtttacccc ggggaaaaca tctctttttg cggtgtttgc
      661 ggcagaatcg cgttcagcgc gttttagcgg tgcgcgcaat aaaatgttat gttaaatgtc
      721 tgctgaatgg tttttaggtt ttttcctaac atatgataga ataaaaaaac catatgttaa
      781 tatattgtgt ttttttcaat gaaaatctca aaggtctctg acgaacattt ttctaattac
      841 aacatgttgc cgaatggtaa tgaagaaatt gacctttttg gcttgattag cgttttatgg
      901 gcagcaaaaa taaaaattgt tggggtgatt ctattttttg cttgtattgg ctttcttgtt
      961 tcttttgttt tgccacaaaa atggactagt tctgctgtgg ttactcctgc tgagtctgtt
     1021 caatggagtg aattaaataa ggagctttcc aagcttcatg ttctgggagt ggattttgat
     1081 atcaatcgtg atagcgcatt tgctctgttt attaagaaat ttcagtccgt taattcgttg
     1141 aatgagtata tgagatcatc tccttatgtt atggagatta ttaaaaagaa taacattagc
     1201 gctctcgaac tgcatcgtgc aattgttgag ttgagtaaaa atatgaagtc gattgatgat
     1261 aatgccagta agaaaaacga caagtcatca ttgtatgtat catggactct gagttttact
     1321 gctccaacaa gtaaagaagc tcacgatgtg ttgtctgggt atattaatta tgtttcttcc
     1381 cttgttgtaa gggatttgat ggaagatata agaaataaac tagaagttaa aactaatgtt
     1441 gaaaaagaaa ttcttgcact ggatgagata aaaattagaa accagctgaa tgcagatatt
     1501 cgacgcctca attattcact ggaggttgct aatgcggctg gaataaaaaa acctgtatac
     1561 agcaatggtc agattatgaa ggatgaccca gattttcctg tggctctcgg ttctgatggt
     1621 atagcaacta aattgaacat caaaaaatca atcaaggatg tttcggaatt gagtggggag
     1681 ttgcgaaatc gtcaatatgt tgtgaatcaa ttggttgtgg cgaaagtggg ggatgttgat
     1741 tttatgccat ttcagtatca attgagccca acgttaccgg tcagaaagga tgggccaagt
     1801 aatgccataa ttgtaattct ttcttctgtc attggaggga tgcttgcatg tggctatata
     1861 cttttacgtc atgccatgga ggccagaaag atgagtgtga ttgagtaaaa gttgtgctga
     1921 tgcatcattt tataagcaac ttttataaaa agttcaaagg atcttcttga gatcattccc
     1981 ttacagccgt agtcttctac tctagaaaac gaaaaaacca cctggggagg tggtttttcg
     2041 aaggttcagt aagttgggga acttctgaac cgtggtaaca gtcttgtgcg agacatgtca
     2101 ccaaatctgt ccttctagtg tagcctcagt aaggctgcca cttcaagaac tctcaataca
     2161 tctctcgcac atcctgttta ccagtggccg ctgccaatgg cgttaagtcg tgtcttaccg
     2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2281 cgtgcacaca gcccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     2341 tgagacgaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     2401 gagcgcacga gggagccacc agggggaaac gcctggtatc tttaagtcct gtcgggtttc
     2461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2521 aaaacgccac cggcgcggcc ttttttcggg gcctcttttt tgctcacatg ttttttctgg
     2581 tgttatcccc tgattctgtg gataaccgta tatccgtctt tgcgtgagca gacaccgctc
     2641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga
     2701 agctgcattt tctccttacg tatctgtgca gcatttcaca cctcatatgt cgctgttctc
     2761 tcaccatctg ctctgccgcc tcataattaa gccagtatac actccgttat cgctacgtga
     2821 ctgggtcatg gctgcgcccc gacacccgct aaaaccccct gacgcgccct gacgggcttg
     2881 tctgctgacg aaaacgccca cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc
     2941 ctgtttcact ttcggcctca aagcgaatct ggatgctgtt ctggagttct tctgcgagtt
     3001 cgtgcagtcg tttgcacatg gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa
     3061 gcagtgtcag gctctggctt ttgatacatc gcgccatatt cagcgcagtt tgttgttatt
     3121 tgttcacctt ccgttgttct ccgtca
//
LOCUS       DBBBSB010000253         3124 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.259, whole genome shotgun sequence.
ACCESSION   DBBBSB010000253 DBBBSB010000000
VERSION     DBBBSB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.259"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            47..1147
                     /gene="ompC"
                     /locus_tag="ACIUS0_004281"
     CDS             47..1147
                     /gene="ompC"
                     /locus_tag="ACIUS0_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGB0221765.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFSLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1311..1796
                     /locus_tag="ACIUS0_004282"
     CDS             1311..1796
                     /locus_tag="ACIUS0_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0221766.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            1906..3124
                     /locus_tag="ACIUS0_004283"
                     /pseudo
     CDS             1906..3124
                     /locus_tag="ACIUS0_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT         1047 a          624 c          542 g          911 t
ORIGIN      
        1 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
       61 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
      121 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
      181 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
      241 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
      301 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
      361 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
      421 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
      481 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
      541 agggctgaac tttgccgcgc aatatcaagg caaaaataaa cgtactgaca acagtcatct
      601 ttatggtgct gacgacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
      661 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
      721 aagagccgca gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
      781 tataaagtat gatgccaaca acatctactt tgcagctaat tacgatgaaa cattaaacat
      841 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
      901 atatcaattc gacttcagct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
      961 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
     1021 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
     1081 ctttacccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
     1141 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
     1201 gtatgactcc ttaagtaaaa atgagcaatt cttgatattt ctggtcattc acctcaatta
     1261 atattattcc atatcctccg gggacatctt actataatat ttattgatga gtgattatga
     1321 ccaatattaa tacagcttgt gtaaaaaata atgccagtta tcaacttaat aacgcattac
     1381 ctaacaaaga aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataaat
     1441 cggttaataa tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt
     1501 cgaacatggc atctcttgat ttgtcatatc ttgatttgag tgaattaccg cctatcccct
     1561 cgacagttaa tacgttaaat ctggaaaaca attgtctaac ttgtcttgac ttagctgaca
     1621 atgccagtct cgttaatatc aacctcagcg ttaataaaat taacacgata acctttccaa
     1681 aagaaccaaa actggaaaac atttatatta atcataacaa tctggaaagt ttagatttaa
     1741 aaaatcagca ttcattggtt aatctggaag cgcaaaataa tagcctaaaa aaatgatatt
     1801 tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat
     1861 gtctcccggc aagaatctct gattgagtta agtgcccatc acaatatgat cactgacctt
     1921 atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat
     1981 ttaaacgcca aaaacactac aaaactggaa tatttaaact taagcaataa caatttactg
     2041 ccaaccaatg acattgatca actaatatca tcaaaacatc tttggcatgt attagttaac
     2101 ggcatcaaca atgatccact tgcacaaatg cagtactgga ctgcagtaag aaatataatt
     2161 gatgacacta atgaagtgac cattgattta tcaggactta atttaaccac tcaaccacca
     2221 gggctgcaaa acttcacctc tatcaatctt gataataacc aactcacaca ttttgatgca
     2281 accaactacg acagactcgt aaagctaagt ctgaatagta atgctcttga gtcaataaat
     2341 tttcctcaag gcagaaatgt aagtattaca catatatcta tgaataataa tgctctcaga
     2401 aatattgata tagataggct ttcatcagtt acttatttta gtgcggcaca taatcaacta
     2461 gagtttgtgc aattagaatc ttgcgaatgg ctgcaatacc tgaatctcag ccataatcaa
     2521 ttaactgata ttgttgcagg taataaaaat gaactcttac tgctggatct atcccataat
     2581 aaactaacaa gtttacacaa tgtcttattt cccaacttga atacgttact tattaacaac
     2641 aatttgcttt ctgaaattaa aatattctat agcaacttcc gcaatgttca gacattaaac
     2701 gctgctaaca accagttgaa atatataaat cttgatttcc tgacttatct tccatctatc
     2761 aaaagtttaa gactggacaa taataaaata acccacattg atactaataa tacatccgat
     2821 attggaactt tattccccat aataaaacag agcaaaaact taaatttttt aaatgtttct
     2881 gggaagaaca attgacctac tatgcagctc atgttattta atttgttttc cccagcactt
     2941 aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga
     3001 atagatgcgg ataacgaatt gtttcactat actattaaaa aagcatatac cccatataat
     3061 atacacactt acaagacaga ggaagttgta aaccagagga atataaaagt taaaaacatg
     3121 acct
//
LOCUS       DBBBSB010000254         3112 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.260, whole genome shotgun sequence.
ACCESSION   DBBBSB010000254 DBBBSB010000000
VERSION     DBBBSB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.260"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACIUS0_004284"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACIUS0_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HGB0221767.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACIUS0_004285"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACIUS0_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACIUS0_004286"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACIUS0_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGB0221768.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACIUS0_004287"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACIUS0_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGB0221769.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACIUS0_004288"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACIUS0_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DBBBSB010000255         3099 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.261, whole genome shotgun sequence.
ACCESSION   DBBBSB010000255 DBBBSB010000000
VERSION     DBBBSB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.261"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(106..1815)
                     /locus_tag="ACIUS0_004289"
     CDS             complement(106..1815)
                     /locus_tag="ACIUS0_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HGB0221770.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="ACIUS0_004290"
     CDS             complement(2247..2966)
                     /locus_tag="ACIUS0_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HGB0221771.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1039 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DBBBSB010000256         3069 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.262, whole genome shotgun sequence.
ACCESSION   DBBBSB010000256 DBBBSB010000000
VERSION     DBBBSB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.262"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..498
                     /locus_tag="ACIUS0_004291"
     CDS             <1..498
                     /locus_tag="ACIUS0_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0221772.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACIUS0_004292"
     CDS             578..1273
                     /locus_tag="ACIUS0_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGB0221773.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACIUS0_004293"
     CDS             1302..>3069
                     /locus_tag="ACIUS0_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGB0221774.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DBBBSB010000257         3041 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.263, whole genome shotgun sequence.
ACCESSION   DBBBSB010000257 DBBBSB010000000
VERSION     DBBBSB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.263"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            2..2905
                     /locus_tag="ACIUS0_004294"
     rRNA            2..2905
                     /locus_tag="ACIUS0_004294"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          803 a          670 c          949 g          619 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc c
//
LOCUS       DBBBSB010000258         2963 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.264, whole genome shotgun sequence.
ACCESSION   DBBBSB010000258 DBBBSB010000000
VERSION     DBBBSB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.264"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACIUS0_004295"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACIUS0_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGB0221775.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACIUS0_004296"
     tRNA            complement(621..695)
                     /locus_tag="ACIUS0_004296"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACIUS0_004297"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACIUS0_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACIUS0_004298"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACIUS0_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGB0221776.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          732 a          713 c          703 g          815 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cag
//
LOCUS       DBBBSB010000259         2952 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.265, whole genome shotgun sequence.
ACCESSION   DBBBSB010000259 DBBBSB010000000
VERSION     DBBBSB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.265"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACIUS0_004299"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACIUS0_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGB0221777.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACIUS0_004300"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACIUS0_004300"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGB0221778.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..>2952
                     /locus_tag="ACIUS0_004301"
     CDS             2604..>2952
                     /locus_tag="ACIUS0_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGB0221779.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          701 c          779 g          813 t
ORIGIN      
        1 aaaggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg ggggcgaggt gctgtatgct
      841 gatatggggc actttggtaa gttctctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aa
//
LOCUS       DBBBSB010000260         2938 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.266, whole genome shotgun sequence.
ACCESSION   DBBBSB010000260 DBBBSB010000000
VERSION     DBBBSB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.266"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..135)
                     /locus_tag="ACIUS0_004302"
     CDS             complement(<1..135)
                     /locus_tag="ACIUS0_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221780.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     Q"
     gene            229..315
                     /locus_tag="ACIUS0_004303"
                     /pseudo
     CDS             229..315
                     /locus_tag="ACIUS0_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
     gene            335..1441
                     /gene="nanM"
                     /locus_tag="ACIUS0_004304"
     CDS             335..1441
                     /gene="nanM"
                     /locus_tag="ACIUS0_004304"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGB0221781.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1506..2486
                     /locus_tag="ACIUS0_004305"
     CDS             1506..2486
                     /locus_tag="ACIUS0_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGB0221782.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            2596..2801
                     /locus_tag="ACIUS0_004306"
                     /pseudo
     CDS             2596..2801
                     /locus_tag="ACIUS0_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          830 a          642 c          701 g          765 t
ORIGIN      
        1 ctggctggct gctacaccat gttgccgggc aacgagggag accgtcatcc ccggttcaaa
       61 gctctgctga acaattgcga tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc
      121 taagacatca atcatctgtt ctccaatgac tagtctaaaa actagtatta agactatcac
      181 ttaaataagt gatactggtt gtctggagat tcagggggcc agtctaatga ctaccttgac
      241 cgccagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg
      301 tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaactataa tggcgcttgc
      361 tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt
      421 taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg
      481 tacagcatgg tacaagctag atacacaggc caaagataaa aaatggactg cgttagctgc
      541 attccctggt ggaccgagag agcaagcaac ctctgcattt attgatggca atctgtatgt
      601 gtttggcggc attggcaaaa acagcgaggg attgactcag gtatttaatg acgtacacaa
      661 atacaacccc aaaaccaata gctgggttaa attgatgtcg cacgcgccga tgggcatggc
      721 gggtcatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa
      781 tatcttcaat ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctataga
      841 taaaatcaac gcccactatt ttgacaaaaa agcagaagat tatttcttca ataagtttct
      901 gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg
      961 aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc
     1021 caaaccagga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa
     1081 atggaataag cttgatcccg tctcatcacc agatggcgtc gctggcggtt ttgcggggat
     1141 aagcaatgat tctcttatat ttgctggagg ggccggattc aaaggttcac gagaaaatta
     1201 ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca
     1261 tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg
     1321 agtatcattg ccctggaata atagtttatt aattattggc ggtgaaactg caggcggcaa
     1381 agcggtgacg gattcagttt taatttctgt gaaggataat aaagtcacag tacaaaacta
     1441 acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttgta ttacgcgaaa
     1501 attatatgaa cgcaataata tcacccgatt attactatgt tcttaccgtt gctggtcagt
     1561 ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat gcgcctcatt
     1621 ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg ccatgtcact
     1681 ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg cagggttatc
     1741 accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata
     1801 ttgcacggaa attactgccc ttcattcctg ataatgcagg gattcttatc gttccgtgtt
     1861 gccgtggcgg atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag
     1921 ccagccatga tgcttgtcgt tggggaacgg atactccgct gtaccaggat ttagtcagca
     1981 gaacgcgagc cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc
     2041 aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata
     2101 tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg
     2161 acgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc ccgcattcgt
     2221 atgaagctat ttatggcaat tatcaaaata atgttttagc caatattatt ttcgtcgact
     2281 tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa
     2341 gcacgggata ttacggttca gcgtaccggt caccggagaa ctggacgacg gcactgcgaa
     2401 gcagtcattt cagcacggca gcccgtcggg ggattatttc tgacagattt gtagaagcaa
     2461 ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt catgggggat gtccttgttc
     2521 gttctcagag tagacaacaa aaaacaaatg agatcaaggt aatatctctt cacttgcggg
     2581 ctcgaacagc aggcaattgc caatataata atctggcaca agggtgaaaa acgcgcaccg
     2641 cataaaccgt tgttattgct tacagattag ccggatgcct taatagacat ccgcgccttt
     2701 tcgattatgg ctcggaaatc tacgaacatc tacacagttt actggaacgt tttgggccac
     2761 agcgttcgca gcaccgtcct gatatgccgt tctggcgatt ataagtcgac ggattctggc
     2821 aaatagacga tgatgatatg cttaatgatg ttcaaagaaa ttaggccatt attatattat
     2881 tgcttgcatt ttctttcatt cctaaaagca cccgccatta atcccaggcg cattatgc
//
LOCUS       DBBBSB010000261         2932 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.267, whole genome shotgun sequence.
ACCESSION   DBBBSB010000261 DBBBSB010000000
VERSION     DBBBSB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.267"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACIUS0_004307"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACIUS0_004307"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGB0221783.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACIUS0_004308"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACIUS0_004308"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGB0221784.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACIUS0_004309"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACIUS0_004309"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGB0221785.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACIUS0_004310"
     CDS             complement(1895..2809)
                     /locus_tag="ACIUS0_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGB0221786.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          773 a          681 c          781 g          697 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattaccta gaaccacggc agcctgacgg ctgcaaattg aaaactgccc ct
//
LOCUS       DBBBSB010000262         2932 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.268, whole genome shotgun sequence.
ACCESSION   DBBBSB010000262 DBBBSB010000000
VERSION     DBBBSB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.268"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACIUS0_004311"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACIUS0_004311"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGB0221787.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACIUS0_004312"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACIUS0_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGB0221788.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACIUS0_004313"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACIUS0_004313"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGB0221789.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACIUS0_004314"
     CDS             2756..>2932
                     /locus_tag="ACIUS0_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221790.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DBBBSB010000263         2894 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.269, whole genome shotgun sequence.
ACCESSION   DBBBSB010000263 DBBBSB010000000
VERSION     DBBBSB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.269"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..308)
                     /locus_tag="ACIUS0_004315"
     CDS             complement(<1..308)
                     /locus_tag="ACIUS0_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGB0221791.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACIUS0_004316"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACIUS0_004316"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGB0221792.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACIUS0_004317"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACIUS0_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGB0221793.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DBBBSB010000264         2842 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.270, whole genome shotgun sequence.
ACCESSION   DBBBSB010000264 DBBBSB010000000
VERSION     DBBBSB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.270"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..203
                     /locus_tag="ACIUS0_004318"
     CDS             <1..203
                     /locus_tag="ACIUS0_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221794.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACIUS0_004319"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACIUS0_004319"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGB0221795.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="ACIUS0_004320"
     CDS             2102..2818
                     /locus_tag="ACIUS0_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGB0221796.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          788 a          626 c          631 g          797 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tt
//
LOCUS       DBBBSB010000265         2809 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.271, whole genome shotgun sequence.
ACCESSION   DBBBSB010000265 DBBBSB010000000
VERSION     DBBBSB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.271"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(430..1209)
                     /gene="ipaJ"
                     /locus_tag="ACIUS0_004321"
     CDS             complement(430..1209)
                     /gene="ipaJ"
                     /locus_tag="ACIUS0_004321"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HGB0221797.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1406..2215)
                     /locus_tag="ACIUS0_004322"
                     /pseudo
     CDS             complement(1406..2215)
                     /locus_tag="ACIUS0_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2562..>2809
                     /locus_tag="ACIUS0_004323"
     CDS             2562..>2809
                     /locus_tag="ACIUS0_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221798.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          847 a          695 c          450 g          817 t
ORIGIN      
        1 ttttaaaatc atattaatta ttaacaagag atttattttt ctaaccccaa aagttatgct
       61 tggtcaaaga gtaacttata atatccatac ccctcatagg gcttacagtt taaattgcgg
      121 gtataaaata catctatttt caaataaaat aaagcatact ccaattacaa cgagtgacta
      181 aaataaaaat atcatccaag tcaatcactg tttgctctgc aaaatatgtc agccttctca
      241 aacttaccag cacttaaata tttaagcaca caatcattcc ttgagggagt tcacttattt
      301 caagagaaac atttcgtctt cccacacaac taatccgccc aatatagcta aatttgtact
      361 cagtaatatc atttgttatg ccccccaccc caaaatattc aagctccttc acagataaaa
      421 aacgacaatt tacaaagcct cattagttat aactatggaa atacctattt tagtatatcc
      481 tataaagtta gaattacttc ttcttgcacc taattccata gttgaaaaac aagaataatt
      541 ttctcctaca gctggatcca tatagcttcc atcaggtcta cacaacaccc agtggaggcc
      601 gacaggaact ccaataaagg atacagcaac agcttccaaa actctttggt tagaagataa
      661 cacatctcgt tgatgtacta tattacatcc catgccaatt aataagtccc ttgcatcggg
      721 ataaataaaa ctaattactt gcgaaaaaat gttactttct tcaacgacat aagcattcaa
      781 tccaagaagc ctcgtagcca tgactatctt atcaggcata gaataaccgg catccgcgat
      841 attatctttg tgtattctcg gattataatt accactagtt attaagtata aatcccgctc
      901 acaacgatta tccaaatcaa gagaactctt ccttgtcatt tcggacatag accctttgta
      961 ttgtggtatt ttatcaaccc ctaattcctt tgcagcacag agtaaactgg cagctccaca
     1021 agaattgtca tacatctgct tcctcgccaa aaaacaacct tgttcgttta ctctattctc
     1081 atttaccctc acatcagtct gatgagatat catatattct cttggtatag ccccctgcaa
     1141 taaaacccca tataacataa cacctgtatg aatacatccc cgtttgcaag gcttccgttg
     1201 ttccgacatc gacgagtaat tgcagcacag tattgcatcc agcaatgcga actactatga
     1261 gaattagctg accgggtcct tattccccga acgaacttta aatccaaaac ctcatactct
     1321 tttccgtcgt catcacgtcc gtcaccataa caacctttca gcatccaccc ttttattaag
     1381 agttaatgtc catcagcatc agcaattaat accgcctgat actgacatat ttttgtcgta
     1441 tttacgtcca taatcggtta ttgtgataca ggtcacacca gattatttgc tattgttaca
     1501 ataaccacct tggttgagag ctgttttttc aactttgtta tcaactcccc caactccttg
     1561 gtcagtaagg ctgccgaccg agcacagtga atagatagta tccgcaggta ccaccatgct
     1621 tacttaatac tcagaaggcg aagtgctctc caattaactc tgccgcacta tgcttataag
     1681 ctgcttttca atgccgtcta tcaacatgtc caattcattc aatctgttgt actggtaatc
     1741 caacgcagta atgatatatg caggtattct ttcatccact ccagtaacgt ttcatgcaaa
     1801 gcattaattt acgcgctacg gaacttcacc agttgactga gcaaacgatg tgggaccagt
     1861 actgcctgca gttcttctat tgttacagcg atttctttac caaacagctg aaccaccatc
     1921 aagataaaga tagcctgaac attcatcgca ccattttgtt accaatggca aaagatttaa
     1981 cgaaccgtgc ctgcatcagg cggacttttt atccctgctt tgttagctcc ctaaccttgt
     2041 acagagagcc tccataagct cccatatcaa gcagacatgg ttcgcagtta ctgacataca
     2101 ccagaaaact ctgacagcag actcacttat cctccacttc actcatatcc ttattggtga
     2161 gatgaacctg aatgacatgt tttgcgacat aaacgccaac cagtgtatat ttcacctgta
     2221 gagattctac aatctgtagc gttatgcatc taatgttagg ctctatgatg tcagaaatct
     2281 gcgagactct acaccttctg catttacctt tatttatcac catgctcctc agttaacgga
     2341 gaacgtgttc attgcatttt gtcacttaca tttcgccttt ggtatcgggg ggatataggt
     2401 tccaacgttc cgcatgagaa cctgaaccgg cttcacgcca ccttgatacc agatgccgca
     2461 caggtagtca acaagtgtca cgaacggtgc aatagtgatc cacacccaac gcctgaaatc
     2521 agatccaggg ggtaatctgc tctcctgatt caggagagct tatggtcact tttgagacag
     2581 ttatggaaat taaaatcctg cacaagcagg gaatgagtag ccggacgatt gccagagaac
     2641 tggggctctc ccgcaatacc gttaaacgtt atttgcaggc aaaatctgag ccgccaaaat
     2701 atacgccgcg acctgctgtt gcttcactcc tggatgaata ccgggattat attcgtcaac
     2761 gcatcgccga tgctcatcct tacaaaatcc cggcaacggt aatcgctcg
//
LOCUS       DBBBSB010000266         2807 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.272, whole genome shotgun sequence.
ACCESSION   DBBBSB010000266 DBBBSB010000000
VERSION     DBBBSB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.272"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            6..81
                     /locus_tag="ACIUS0_004324"
     tRNA            6..81
                     /locus_tag="ACIUS0_004324"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACIUS0_004325"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACIUS0_004325"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACIUS0_004326"
     CDS             complement(299..1057)
                     /locus_tag="ACIUS0_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGB0221799.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACIUS0_004327"
     CDS             complement(1065..2168)
                     /locus_tag="ACIUS0_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGB0221800.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACIUS0_004328"
     CDS             complement(2178..>2807)
                     /locus_tag="ACIUS0_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGB0221801.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DBBBSB010000267         2802 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.273, whole genome shotgun sequence.
ACCESSION   DBBBSB010000267 DBBBSB010000000
VERSION     DBBBSB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.273"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(118..720)
                     /gene="fimB"
                     /locus_tag="ACIUS0_004329"
                     /pseudo
     CDS             complement(118..720)
                     /gene="fimB"
                     /locus_tag="ACIUS0_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            2174..>2802
                     /gene="nanC"
                     /locus_tag="ACIUS0_004330"
     CDS             2174..>2802
                     /gene="nanC"
                     /locus_tag="ACIUS0_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGB0221802.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIE"
BASE COUNT          900 a          560 c          511 g          831 t
ORIGIN      
        1 attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt taatcatttt
       61 tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca actatctcta
      121 taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa aacgccctgc
      181 attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat aatcctagat
      241 aagtcgcgta tctattccca tattcgccaa agcaaaacca cacgaatagc gtaacatgtg
      301 cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa tatggtaaaa
      361 ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc tctcagcatg
      421 cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt tgttcaatag
      481 cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact ttgctttaag
      541 atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat gaataaaaca
      601 aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg ctttaaggag
      661 tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct tattcctcat
      721 cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat
      781 ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg taagtggacc
      841 agttcttctg agcgaactta aatggagtag cgatagttac ttataataac tttgccatac
      901 aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga tacgaatcta
      961 ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt
     1021 aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttaaa ctagccgtct
     1081 caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca tggaacgcag
     1141 taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta ttcctgcctg
     1201 gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga tatgggggaa
     1261 cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc actacgactt
     1321 aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta agtgaaacat
     1381 catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag tgaccagtta
     1441 caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct gatggcgagt
     1501 aaacccttat tttcaccact ctcactgagt tagcttgcaa agctttcttt gtttagattc
     1561 taaataaatt atttataaaa cgtatagtta aaagaaaatc tttagttttt gaagaccacc
     1621 gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg acatattttg
     1681 tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga tctggtataa
     1741 caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat ctatggcaaa
     1801 catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga aaggaatatg
     1861 aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt gagtaagtga
     1921 tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac tcaggtattt
     1981 aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg agaccttata
     2041 caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg caaaaaaata
     2101 ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa
     2161 atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc
     2221 gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag
     2281 ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag
     2341 catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa
     2401 tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga cggtgcgccc
     2461 gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc
     2521 ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta
     2581 ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata
     2641 tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa
     2701 acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg cctttgttct
     2761 acaataccat atgacgcccg atattacgcc atacatagaa ta
//
LOCUS       DBBBSB010000268         2764 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.274, whole genome shotgun sequence.
ACCESSION   DBBBSB010000268 DBBBSB010000000
VERSION     DBBBSB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.274"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..491
                     /locus_tag="ACIUS0_004331"
     CDS             <1..491
                     /locus_tag="ACIUS0_004331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221803.1"
                     /translation="LGKTMRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSP
                     SNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYYVIA
                     NNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKIMYL
                     SN"
     gene            552..923
                     /locus_tag="ACIUS0_004332"
     CDS             552..923
                     /locus_tag="ACIUS0_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221804.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            1333..1710
                     /locus_tag="ACIUS0_004333"
     CDS             1333..1710
                     /locus_tag="ACIUS0_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221805.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            1733..1885
                     /locus_tag="ACIUS0_004334"
                     /pseudo
     CDS             1733..1885
                     /locus_tag="ACIUS0_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1941..>2764)
                     /locus_tag="ACIUS0_004335"
     CDS             complement(1941..>2764)
                     /locus_tag="ACIUS0_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HGB0221806.1"
                     /translation="SHASFQEKEAGDGSTQHIAVSTESVVDDEHLQLDDEETANIDTD
                     TRVIEAEPTWISFSRPSRIEKSRRARKSSQTILEKEEATTSENSNLVSTDEPHLGGVL
                     AAADVGGKQDATNYNSIFANRFAAFDELLSILKTKFACRVLFEETLVLPKVGRSRLHL
                     CKDGSPRVIKAVGVQRNGSEFVLLEVDASDGVKMLSTKVLSGVDSETWRNDFEKIRRG
                     VVKSSLNWPNSLFDQLYGQDGHRGVNHPKGLGELQVSREDMEGWAERVVREQFTH"
BASE COUNT          726 a          694 c          611 g          733 t
ORIGIN      
        1 agctggggaa gactatgcgc gatctgttga aggtggttct aagcctcgta cttgcgatgg
       61 catcggggca ggcacttgct gacctgccaa ttgttttagt ggatgaagct cgtcttccct
      121 atgactactc cccatccaac tacgacaact ccataagcaa ttacgacaat agtccatcaa
      181 attacgacaa ctctgagagc aactacgata atagttcatc caattacgac aatagtcgca
      241 acggaaatcg taggcttata tatagcgcaa atgggtctcg cactttcgcc ggctactacg
      301 tcattgccaa caatgggaca acgaacttct tttccacatc tggcaaaagg atgttctaca
      361 ccccaaaagg ggggcgcggc gtctatggcg gcaaagatgg gagcttctgc ggggcattgg
      421 tcgtcataaa tggccaattt tcgcttgccc tgacagataa cggcctgaag atcatgtatc
      481 taagcaacta gcctgctctc taataaaatg ttaggcctca acatctagtc gcaagctgag
      541 gggaaccact agtgtcatac gaacctccaa gagacggtta cacaaacggg tacattgttg
      601 atgtcatgta tgacaatcgc ccaagtaagt atccagctgt gttcagaacg tacgtccgaa
      661 ttccagacta tgtcgaacta gataacgcgg aggagctggc cgacgcaatc attcgagagg
      721 ttgagttaaa ggaggcatgg ctcacaaggg tcttggggaa ctcagccgat cctgacgctt
      781 ctgtgggcaa gtgggaccgg cgcctagcgc agtgcggtgg tattgatgag atgtcaagtg
      841 gggtgcaggc aacatacttc agtccaaacg gtcagctggt cggcttagtg gcatacattg
      901 atgccgaatg gatggatgaa tagctctgac gttacgattc aggtgagaca accagatggg
      961 cattgcgtac aagctcagtt gccaccgatg tggctacgag tctgatcttc tttacctagg
     1021 ccaaggtatg gcgatgcttc ctgagcaaat agtcggaacg tgcacatgct gcgacaatct
     1081 aacgacgatt tctataaatg aaagctacgg agtatgctca ctttgtgggg ctaaggatcg
     1141 tgtgatattt acaagctatc agcatgaggc aaggccaaga aatccctgct atctagagag
     1201 aagtttcaaa tatcaatgcc caagatgcca tgccttcagt atggagcctc cggatctacc
     1261 ggagatgctt tgggactaat gctccgcttt gcctcgggta tccgttcgac ctatgtttaa
     1321 gaggaaacgc acgtgtctgc cgccaaagct cttgcctatg cactttgtga caagttggaa
     1381 agcaaaaaag ttgatgaagt cgtacgaaat cttgtttcta gcggctggaa tattagggaa
     1441 gtggcctggg aagcttcgtc ctttgatcat gaaatgccct actttgacac ggggcgggac
     1501 ttcgatattg gatttcctga cacgctgtca tccaagattg agtttttgac cgcgaatgga
     1561 tcaatcgtcc aagcacgtgc tcgcctgcac ttcaagcagg ctctaatttt tgccaaggcg
     1621 tccaaacatg tcgccgcgct caagaactca ttggattact actatggatc gtctgtaatc
     1681 ccccctcaaa accacggagg ctatttgtag aaaattccat taatatgaac tcaaggtgtt
     1741 ccctacggac gaatcagcga tgaaggtggt gtacctggct atccaggcgg cgaccaagaa
     1801 atggacgatg ccgatccgca actggaagcc ggccatgaac cgttttatga tcgaatttgg
     1861 tgaccgttta aatggccacc tttaactgac cggcaaaagc acttacacaa aagggtttac
     1921 aggctctttc agtcattcct ttaatgcgta aattgctctc taaccactct ctcagcccac
     1981 ccttccatat cctctctcga tacttgcagc tcccccaacc cctttggatg attcacccct
     2041 ctatgcccgt cttgtccata taattgatca aacaaactat ttggccaatt caatgagctc
     2101 ttcaccactc cacgccgtat cttttcaaaa tcattccgcc atgtttcgct atcaacgcca
     2161 ctcaacactt tggtagaaag cattttcacc ccatccgatg catccacctc tagcaataca
     2221 aattcactgc cattacgttg caccccaacg gctttaatca ctcttggtga gccatcttta
     2281 cacagatgta atcggctacg cccaactttt ggcaaaacca aggtttcttc aaaaagcacc
     2341 cgacatgcaa atttagtttt tagaattgaa agtagctcat caaaagcagc aaatcgatta
     2401 gcaaaaatag agttgtaatt ggttgcatcc tgcttcccac ccacatctgc cgctgctagg
     2461 acaccaccta agtgtggctc atcagtacta accaaattac tattttcact tgttgttgct
     2521 tcttcttttt ctaaaatagt ttggctactt tttcttgccc tgcgagattt ttcaattcga
     2581 ctaggtctac taaaacttat ccatgtcggc tcagcctcta tgactcgtgt gtctgtgtct
     2641 atattggctg tttcctcatc gtccaactgt agatgctcat catcaacaac tgactctgtt
     2701 gaaaccgcta tgtgctgcgt actaccatca cctgcctcct tttcctgaaa agaggcatgg
     2761 ctaa
//
LOCUS       DBBBSB010000269         2760 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.275, whole genome shotgun sequence.
ACCESSION   DBBBSB010000269 DBBBSB010000000
VERSION     DBBBSB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.275"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..198
                     /locus_tag="ACIUS0_004336"
     CDS             <1..198
                     /locus_tag="ACIUS0_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGB0221807.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="ACIUS0_004337"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="ACIUS0_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGB0221808.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="ACIUS0_004338"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="ACIUS0_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGB0221809.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="ACIUS0_004339"
     CDS             1822..2577
                     /locus_tag="ACIUS0_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGB0221810.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          686 a          640 c          682 g          752 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggagttatgt tcaacaattt
     2701 aattttagat atacatataa cttgttctat taataaatca cctcgtattc tgactcggcc
//
LOCUS       DBBBSB010000270         2744 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.276, whole genome shotgun sequence.
ACCESSION   DBBBSB010000270 DBBBSB010000000
VERSION     DBBBSB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.276"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..523
                     /locus_tag="ACIUS0_004340"
     CDS             <1..523
                     /locus_tag="ACIUS0_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221811.1"
                     /translation="HDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKAR
                     SVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGRVEIDNNIGENA
                     LRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQ
                     VHELLPWNFSSVK"
     gene            complement(777..941)
                     /locus_tag="ACIUS0_004341"
     CDS             complement(777..941)
                     /locus_tag="ACIUS0_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221812.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1843..2127
                     /locus_tag="ACIUS0_004342"
     CDS             1843..2127
                     /locus_tag="ACIUS0_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGB0221813.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            2115..2600
                     /locus_tag="ACIUS0_004343"
     CDS             2115..2600
                     /locus_tag="ACIUS0_004343"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0221814.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          720 a          586 c          787 g          651 t
ORIGIN      
        1 ccatgacgag gatgtgcgcc gtccgacaga aatgactcag gaagcgctca gacggatagc
       61 agagttatac gacatagaag cggagatacg tggcagtccg gcagaggaac ggcttgcagt
      121 cagaaaagcc agaagcgtcc agttgatgca gtcgttgtac gactggatac agttgcagag
      181 gaaaacgctg tcgaaacatg cggagatggc gaaggcgttc gactatatcc tgaatcactg
      241 gaatgcgctg aacgagttct gtcgtgacgg ccgggtggaa atagacaaca acatcggtga
      301 aaacgcgtta cgatcggtgg cggttggaag aaaaaattat ctctttttcg gctcagacaa
      361 gggaggagaa agtgcggcga tcatctacag tctgctggtc acctgcaaac agaacgaagt
      421 ggagccggag gactggttgc gcgaagtgat cgagaagctc aatgactggc cgtcgaacca
      481 agtgcatgaa ctgctgccct ggaacttctc gtctgtaaaa taatccttac gctacgtact
      541 tctcggggcg cttaccttca aaatgatgga cattattttt gtggaaccgg aggtaacaga
      601 ccagacggtt taaatgagcc ggttacttta aacgggtgat ggacagcacc tcaaaagttc
      661 agatggatta tatttgtctg caataccccg ggtattttcg atttgcgaaa tggatggaac
      721 gacttgctca ggggatagct gatggagtta ctgaagtacc gaaggaccac taaaaatcac
      781 aggccgggaa tttcggcgtt tccggcgtag gtgtgccacg aatgcatcca gtggcgtgat
      841 gttggatgca atgctgtatg agagatgagg ggcggcgtcc agacgcagcc ttcaaaccgc
      901 cttgagctgc tgccgttaag agcggtggtg gtgagcgtca aggttaaaag gcgcagaaca
      961 tgtgtcaggt aggtgttgcg gagatgaacg aaagtgaact gtcgatgaag tgtcgaaagg
     1021 aacctctagg cgatgtcgaa aatgtgctat ctacgcagcg catgacgagt ccagcagata
     1081 tctggtactg gctgggcggc atccggcata taggcagcat gaacgcaaca caggctctcg
     1141 tacggaacgt gggaacctgt cgtctcgatg ctaagggagc ggcgcggagc agaggcactg
     1201 caagcgcctg agtaccgatg cgagacacag gggcagatgg cttcgtagta gtgaggaagt
     1261 gcctgtaatg ggcatggagc gaagggggtc agtcgttggg gctggtttat cggtcaaccg
     1321 gatagccggg aggagcccat gagtcagcca aagccgttcg aggtctctaa gtatgcggtt
     1381 tggaaagcat accagcgggt caaagctaat cgaggtgcgg caggtgtcga tgggcagtca
     1441 gtcgaggcgt ttgaggttac caatcgaagc tatacccggt aatggcgcag gttgacctgc
     1501 acttggccaa gtggattgcc cgcaaacaca agcgagcgcg aggtagtctg gtgcgtgcat
     1561 tcgagtggtt actgcgaatt cgtcacgact gcccgacgct gtttgcacac tggtgcttag
     1621 cttacgatac ttgaatggtt caacgacaag agccgtatga cgcgagagtg tcacgtacgg
     1681 ctccgtggga gcgtaggggg gcggtgcccc tgcgccaccc gatatcccct ttgagtgcgg
     1741 ttacgtccac agaggtaaat gccgctgacg cggcatctgt aatctgttac tttgtgtata
     1801 tcatttgcta tatattgtca atatgctatt gttgtaaagc aaatgatata cggaggcttt
     1861 atgaaatcag gtgttcagct caatctcaga gcaagagaat cacagcggat actcattgat
     1921 gctgctgcag aaattcttca taagtcgcgt acagatttca ttctggaaat ggcctgtaaa
     1981 gctgctgaag atgttattct cgaccggcgg gtgtttaatt ttaatgacag gcaatatgaa
     2041 gagttcattg aaatgcttga tgctccggtt gcagatgatc ctgccatcga aaaattgctg
     2101 gcaaggaaac ctcaatggga tgtgtaactg caccagaacc tttatcctca tttcatcagg
     2161 tcgctgaatt tgtcagcagt gaagctgtac ttgatgactg gttaaagcag aaggagctaa
     2221 aaaatcaggc aatcggagcc acccggactt ttgttgtatg cagaaaaggt acgcaacaaa
     2281 tagtcggttt ttactcactg gccactggta gtgtcaatca tacagaagct acaggtaacc
     2341 ttcggcgcaa tatgccagac cccattcctg taattatact tgcacgcctg gctgtagatg
     2401 tgtcatttcg aggaaaaggg cttggcgcgg atttacttca tgatgcagtt cgtcgttgtt
     2461 acagggttgc cgaaaatatc ggtgtgcgtg caattatggt tcatgcgctt acggaaaacg
     2521 caaaacagtt ttatattcat catggtttta agccatcaaa aactcaggtg caaacattgt
     2581 ttcttaaact ccctcaatag ggagtaatgc cgatcagtta aggatcagtt gaccgatcca
     2641 gtggctgtgt aagaatccgg aaacgctcac tcgtttccgg atttttctgt gcacagtaaa
     2701 agaccacctc ctgaggaggt ggcttttcag aatgccccct ataa
//
LOCUS       DBBBSB010000271         2734 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.277, whole genome shotgun sequence.
ACCESSION   DBBBSB010000271 DBBBSB010000000
VERSION     DBBBSB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.277"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            88..153
                     /locus_tag="ACIUS0_004344"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACIUS0_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACIUS0_004345"
     CDS             381..629
                     /locus_tag="ACIUS0_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGB0221815.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="ACIUS0_004346"
     CDS             complement(745..1014)
                     /locus_tag="ACIUS0_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGB0221816.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="ACIUS0_004347"
     CDS             1071..1739
                     /locus_tag="ACIUS0_004347"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGB0221817.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            2055..>2734
                     /locus_tag="ACIUS0_004348"
     CDS             2055..>2734
                     /locus_tag="ACIUS0_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0221818.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          835 a          589 c          538 g          772 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 atccaaccgc agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt
     1801 gcgactttac ctctattgat aatgcattcc ggcagaacgt tcaaatatga acgtacgata
     1861 tttaactaac cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag
     1921 tatcgttttt ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa
     1981 tcctgaaaca ttttatttca gcttattatt ccccagtatc acattttatc aggatatcct
     2041 tctgcgaggt tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt
     2101 tttacagaac tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat
     2161 ggactaaaaa cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat
     2221 gtctggagaa cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac
     2281 gattgcctga caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg
     2341 agctaccaag ggcattgaaa gagctgaatg caagcagtaa tcaattatct gcacttcctg
     2401 aattaccagt gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg
     2461 aattacctgc gtcactagaa tatattaatg taagtgacaa tcacctgtct gtacttccga
     2521 ggttaccaat gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag
     2581 attttccaga aagagatgat catattataa gaatattctg gcttaatcag aaccggatca
     2641 cggcaattcc ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa
     2701 atcaactatc tcccagaata atgcaaactt tgtt
//
LOCUS       DBBBSB010000272         2714 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.278, whole genome shotgun sequence.
ACCESSION   DBBBSB010000272 DBBBSB010000000
VERSION     DBBBSB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.278"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACIUS0_004349"
     CDS             52..2574
                     /gene="lpfC-O113"
                     /locus_tag="ACIUS0_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGB0221819.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2714
                     /locus_tag="ACIUS0_004350"
     CDS             2585..>2714
                     /locus_tag="ACIUS0_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0221820.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          585 c          679 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcgg
//
LOCUS       DBBBSB010000273         2701 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.279, whole genome shotgun sequence.
ACCESSION   DBBBSB010000273 DBBBSB010000000
VERSION     DBBBSB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.279"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            354..497
                     /locus_tag="ACIUS0_004351"
     CDS             354..497
                     /locus_tag="ACIUS0_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGB0221821.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            510..719
                     /gene="cspE"
                     /locus_tag="ACIUS0_004352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             510..719
                     /gene="cspE"
                     /locus_tag="ACIUS0_004352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGB0221822.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            885..1694
                     /gene="rlmA"
                     /locus_tag="ACIUS0_004353"
     CDS             885..1694
                     /gene="rlmA"
                     /locus_tag="ACIUS0_004353"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGB0221823.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1691..2257)
                     /gene="mntP"
                     /locus_tag="ACIUS0_004354"
     CDS             complement(1691..2257)
                     /gene="mntP"
                     /locus_tag="ACIUS0_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGB0221824.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2254..2376)
                     /locus_tag="ACIUS0_004355"
     CDS             complement(2254..2376)
                     /locus_tag="ACIUS0_004355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221825.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          743 a          619 c          637 g          702 t
ORIGIN      
        1 taattgatag ccgttaactg actgttttat gagaaaaagt gatataactt tttattcatt
       61 gcatagcaga aaatgtgata ttgcacgcac tatgtaataa cttctccctc tggcctggaa
      121 caactgaact tattgaacta tgttagaaac tacgccagtt taagtatctg cctgaactgg
      181 caaggttaag cacaatgata tatcggcgcg tattccgttg cataagtgtg caaaaaaagt
      241 ggaagacgta tcgagatttg tgcgtctgat cgagatatgt ttaaaaatgg cttgccataa
      301 ttaacgttgt atgtgataaa agatttcggg ttaaacgagg tacagttctg tttatgtgtg
      361 gcattttcag taaagaagtc ctgagtaaac acgttgacgt tgaataccgc ttctctgccg
      421 agccttatat tggtgcctca tgcagtaatg tgtcagtttt atctatgtta tgcctgcggg
      481 cgaagaaaac aatctaagga atttttcaaa tggcaaagat taaaggtcag gttaagtggt
      541 tcaacgagtc taaaggtttt ggcttcatta ctccggctga tggcagcaaa gatgtgttcg
      601 tacacttctc cgctatccag ggtaatggct tcaaaactct ggctgaaggt cagaacgttg
      661 agttcgaaat tcaggacggc cagaaaggtc cggcagctgt taacgtaaca gctatctgat
      721 cgaatccact gatctgaagt gtgaatacgc ttcaatctcg ctataaagcc tcgtcgaatg
      781 cgaggctttt tactatgctt tatcttcgct cctggcgttc ggatatttgc ccgccgcgtg
      841 attcgcgtta cacttgcggc ctttagtatc ctgccggagt tgtcatgtcg ttttcctgtc
      901 cactttgcca tcagcctctt tcgcgtgaaa aaaacagcta tatctgtccc caacgacatc
      961 agtttgatat ggcgaaagaa gggtatgtca atctgctgcc cgttcagcat aaacggtctc
     1021 gtgatccggg cgacagcgcg gaaatgatgc aagcacgccg cgcattctta gatgccggac
     1081 attatcagcc gctgcgtgat gcaattgtcg cccaactgcg ggaacggctt gatgaaaagg
     1141 ccacggcggt gctggatatt ggctgtggtg aagggtatta cacacacgca tttgccgatg
     1201 cgttgcccga aatcaccacg tttggcctgg atgtctcgaa ggtagcgata aaagcggcgg
     1261 cgaaacgcta tccgcaggtc actctttgtg tcgcttccag ccaccgtttg ccgttttccg
     1321 ataccagtat ggacgccata atacgtattt acgcgccgtg taaagcagaa gaattagcac
     1381 gagtagtgaa gcccggcggc tgggtcatta ctgccacgcc gggaccgcga catttgatgg
     1441 agctgaaggg gctgatttac aatgaagtac atcttcatgc acctcatgca gaacaactgg
     1501 aaggttttac attacagcag agtgcggagt tgtgttatcc gatgcgtctt cgcggtgatg
     1561 aagccgtcgc attattgcag atgacgccgt ttgcctggcg tgcgaagcca gaagtctggc
     1621 aaacactggc agcaaaagaa gtgttcgact gccagacgga ctttaatatt cacctctggc
     1681 agcgttctta ttaaccgtgg aagtgcgtcc agaggatctg gacgccgatg ccgatcagca
     1741 ccagcccgcc gagaatttcc gcttttttcc caataattga gccgataaag cgaccaacca
     1801 tcatccctaa tgtcgacata atcaaggttg cacaaccaat ggccaatgcg gtcgcgataa
     1861 tgttgacctg aaggaaagca agaccaacac ccacagccat ggcatccagg ctggtggcaa
     1921 tcgcggtggt taccagtagc cagaaaccgt gccgacggcg cggctcttca tcttcatcat
     1981 ctgcgccacg aaaaccctca ataatcattc gcccgccgag gaatatcagc agcacaaacg
     2041 caatccagtg attccattca aggacaaacc ggctggctaa catgcccatt ccccagccga
     2101 tcagcggcgt cagggtttcg acggcaccaa aaataaggcc ggttcgcaat gcttcagaaa
     2161 atttcggttt atggaggggg gcacctttac cgattgatgc agcaaatgca tccatcgaca
     2221 taccaaacgc aagaagaaca gtagcagtga tattcatgac aacgtcctga ccggggtatc
     2281 cataaaacac atcatcgccc ccagcaaaca tgcatacatg aacaacaagc cgcgatgggt
     2341 tttcgcgttc atgcaggcat agcgattgat gtgcatatgg tctcgcctga ctgaaagtct
     2401 tcagtccgta cgtgccacgt tttgcaacga gtatgttgac acgtacattt ccggaatctg
     2461 gaaatcggct actccccaaa tgagggcgca accttaacat attttgagaa tataaaacaa
     2521 ctgttggaat atattatttc attgctttat tgataacgat tttcatttat gtttatatat
     2581 caatgaaacg ttaaaataaa taacaaataa gggaagagat aatatagcct aagctatgtc
     2641 tgattattaa aaatacacta aacatagctt tggctaaatt cataagttat tgagttgata
     2701 c
//
LOCUS       DBBBSB010000274         2693 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.280, whole genome shotgun sequence.
ACCESSION   DBBBSB010000274 DBBBSB010000000
VERSION     DBBBSB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.280"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..971
                     /locus_tag="ACIUS0_004356"
     CDS             <1..971
                     /locus_tag="ACIUS0_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGB0221826.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1552..>2693
                     /locus_tag="ACIUS0_004357"
     CDS             1552..>2693
                     /locus_tag="ACIUS0_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221827.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEK"
BASE COUNT          887 a          536 c          482 g          788 t
ORIGIN      
        1 ccgcagagat gtttgtgttt ctcgccgtcg agctttcgtt gctgtttata gtgattagtg
       61 ccggtgtcag cctgataaga caaaaggtgc cagaccataa aatccagcag atgatggggg
      121 ccagaaaagg gagaggctat ctcctggctg ctctgttggg agccgttacc ccgttctgta
      181 gttgctcgac aatacccatg ttacgtggat tgttgtcagc gaaagccggg tttggtccga
      241 ctctcacttt tttatttgtt tcaccattac ttaatcccat tatcgtcgga ctaatgtgga
      301 tgacctttgg ttggaaagtc accttgttgt acgcgattat cgcagccggc gtctccgttc
      361 ttgccagtat tatcctggat tctctggggt ttgaacgtca tatcattgcc agtaaaagct
      421 catcagcaaa ttgttgtgct ccagcaaaaa cttcgccggg aacgacatat acgcccataa
      481 aagtgagttg ttgtagtcca gcggctaaag ccattgagaa ccccgtagtt acctgttgca
      541 gtaccaaagc tgtagtaagt attaatccca taaaactagc caccaaagat gcgttgcaac
      601 agtttaaaga tgtactgcca tatcttttgt taggagtatt aataggatct tttatttatg
      661 gctttattcc ctcagcgtgg attgccgctc atgcaggagc agataatccc ttcgccatcc
      721 cattaagcgc cgttgtcggt attccgctgt atatccgggc tgaggcggtt atccctctgg
      781 catctgttct gatgacaaaa ggaatgggtc tgggagcatt aatggcatta atcatcggca
      841 gtgccggcgc aagcctgacg gaagtgatat tacttaaatc aatgttcaga atgccgatga
      901 tagctgcatt cctgacggtt atattaggta tggctatctt gatgggctat ttgactcaat
      961 tgctatttta aatagatgcc agcagtaaac tataagatgc atagttagtg ccatatcctt
     1021 ctaagtagaa aatcatagac aggataatcc ttaaaaatca caaatgattt aactaatgca
     1081 ttgagaatga tgacggctcg gtcaacaagt catcaacaga caataaccac gtaatcatgc
     1141 ttctctgaca ttttatcgga gaagcaatga ctctttgtct cagtaccctc ctccttttta
     1201 aaaggcagat ttcagtcgct aatacaacac atttttaaat acactttaac ctggttcatt
     1261 attacacatc taaaacatca caaaaatatt acttcacaca ctatcggaga acataataac
     1321 tttttaaaat gagcgttttt tgatagttgt taacgttttc ctttacgata ctatcactgc
     1381 ccttttttta tacacaaata ttaccgggtg gaaatgaaag ggaatttaaa ggtaattata
     1441 tcaggaagat ataacgcatt atatttattg tgtgtagaag aaaatccatt cctgttctaa
     1501 ttatccaatt taaaaacatt agcataaaac agatgatgaa taaggaatgt tatgtcaatt
     1561 gactttaccc caggtataat aaatacatat cacggcgata tttataactg cacaacaaat
     1621 accgataatg ttaaaacgcc agacacaccg aagtggcctt gtgataattg ggaagaacaa
     1681 caacccatta attccacatt ctctggagaa ggatacaacc ctgaacaatt tgatttagcg
     1741 caacaacaac tccaacaaat taatgcatgc cacacgaaca ccacatatac aaatgcagac
     1801 tactcaaaag ttgtggttca acttgtaagt cttattaata acattgaaac gataagttcg
     1861 acaccactta cgcaacagac acaatcaata cttaatcaga ttcataacat tagatacgaa
     1921 aaggataagg attctgtatg tcaaatcatt gttgtcgtta atccagaacc caacaatcca
     1981 atcataacta aaattctcgt agaagaagga atacccgaaa gattcagtgt ccaaaccgtt
     2041 tcttccgata ataaaaattt tgctgggcaa cgagctgact taccaactga tataagagac
     2101 attcaaagct tgtacttaaa aatggcgaag ctgtatattg agcacagtaa aaatgaaaat
     2161 aggatggaag cccttgctgg atgcgacttt attgatttca acatgacagg gcaagatatg
     2221 tccaagctag tcctaacctt atcaaaattt tattttgaag atctacttaa tataaatttc
     2281 actgacgcaa atttatttaa cactattttt tcacacaagg aaaatcccat ccccaaacta
     2341 cataaatatg aacaacatct tgacaaacaa atcaatggtt tgttttctac attgttaact
     2401 attaatgaca actctttacg cgcgaaagcg gaaattgctt cgagaataat tgatttctta
     2461 gaggcaaagg tagtgaatct ttcatttgat gacatattaa aatataagca agagttcaaa
     2521 aaaatatgtt ataaacaact tcaagagttt acaacaccct ctctatacaa taaaattcaa
     2581 aaatgggcaa caatgtctaa aaatgaattc atagaatttc attacgagac acttcaaccc
     2641 gaaaagattt catacccctt ttatttaaag agagatctgc ctaatgaaaa aga
//
LOCUS       DBBBSB010000275         2666 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.281, whole genome shotgun sequence.
ACCESSION   DBBBSB010000275 DBBBSB010000000
VERSION     DBBBSB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.281"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACIUS0_004358"
                     /pseudo
     CDS             complement(38..583)
                     /gene="ygeQ"
                     /locus_tag="ACIUS0_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
     gene            complement(1245..2234)
                     /locus_tag="ACIUS0_004359"
     CDS             complement(1245..2234)
                     /locus_tag="ACIUS0_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221828.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2318..>2666)
                     /locus_tag="ACIUS0_004360"
     CDS             complement(2318..>2666)
                     /locus_tag="ACIUS0_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221829.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          690 a          620 c          644 g          712 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agcctacgac aaatcgaaga
      841 caaccagcta acctattgtt tcttaaagac aggtgaatcc agttggttgt agtgatagtc
      901 acacatttag agatagaagg tcaagacttg aagcacgtaa tattccctct aaaggaatag
      961 aactcacggt ctgactaccc ggcccttttg gtctggctca ggtcaggcta tgggggcttt
     1021 tggctcgctc tttatattag atgaggtctc acatttttca gtcaaaactc atcgtctaat
     1081 agcccatagc cagaccacca gtcccagctt tagcaggaag accacgagat agactttgtg
     1141 taggtccatt tggccctcct actttctgta ttcacaaact agattactag tacaaaataa
     1201 aggccagaca tccagttcgg aagtctgacc aatagtcgtt attgttagtt agcttcttta
     1261 gtgaagctca ggcgtttgac tacaggcagg acagcagtag ccgagccagt acccatcttg
     1321 atggtcgtag cagcttcagc tacaacaaac accttgccga gaatctcaag gtcgtgagac
     1381 tgggagtctc catcacgtga aaggaccaca gtaatgctct cgccatcgtg acggaagcaa
     1441 ccgaagctca gagccgtaaa acgcttagcc atcatgaaca cgccacggtc ttcaccgttg
     1501 taataggcga cgtcagtccc cagcatatcg acgatagcgt cttcaccacc gaagccctgc
     1561 ttagcagcca gacgggagag agccttgagg tgtgactcat gggcgataat gccgtagtca
     1621 gaaacctcag aaccgaaaga agactccgtg ttctcgtaca gggcatccat gatagcgtca
     1681 gccttagcgg tcaggctacc agtcagggcc ggaagttcta cggtcttcag cagctttgag
     1741 gactccagca cgtcaaccat cttagagtta atggtacgaa tcacgttatc gcagatgttc
     1801 agtgcgtcct gctggagcgt cccctgagtg aaaccagtgg tcgacttctg gtcccaaagc
     1861 tttgtttggt gagacacgag gatcgcctgt tcaatcggct caacggtgat ttcagtgcca
     1921 gacggagcgg tcacaacttc aggtcgctta ccaccacgga cagggaccag cacagagcgg
     1981 tcagccagag tgtgagcaat gattggagtc ggtaagctgt tacgggtcgg caggacttct
     2041 acagggaacg ggatgttatc ttgagcgtct ttaagtacac cgttcagcat agccttaaca
     2101 gtcttagcta actggagggc atagacctga gggtcggcgt tagtcggcag accttcattg
     2161 acctgaatgt tgtcggtatc ggtgatgcgt actggctcaa tgccaaactg gaaagcgttt
     2221 ttagtcatat tcattataat taatctcctt gagtcaaata cttgaagtcc ctaacaggca
     2281 gtcagggacc gtgtgttctc agatgaggga cgcttgctta caagctgtcc atccagttac
     2341 cgcctgactc aaggtcagcg acgatagcgg cacgtgagcg accagcggaa tgagtagcgc
     2401 tgcggaggtt ttcaccatag gtccggcgtt cagctttgac ctgttcgcgg cgctgtagct
     2461 ctttttgcca ttcgtcttcg gtcatgaaca tttcgcgggt acgctctttc tgagacgcta
     2521 gctctttagc gatacgctca gcttcagctc gtttgttcat ctggcggcgt tggtaggcgg
     2581 tcatcaggtc attactgaag aagttaccaa tggtcacgcc agcttcagcc agagcttcca
     2641 gtaactccat cgcctcgcgg ctggtg
//
LOCUS       DBBBSB010000276         2618 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.283, whole genome shotgun sequence.
ACCESSION   DBBBSB010000276 DBBBSB010000000
VERSION     DBBBSB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.283"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..357
                     /locus_tag="ACIUS0_004361"
     CDS             <1..357
                     /locus_tag="ACIUS0_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGB0221830.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
     gene            619..981
                     /locus_tag="ACIUS0_004362"
     CDS             619..981
                     /locus_tag="ACIUS0_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGB0221831.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            978..1907
                     /locus_tag="ACIUS0_004363"
     CDS             978..1907
                     /locus_tag="ACIUS0_004363"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGB0221832.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            1904..>2618
                     /gene="gtrII"
                     /locus_tag="ACIUS0_004364"
     CDS             1904..>2618
                     /gene="gtrII"
                     /locus_tag="ACIUS0_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HGB0221833.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDI"
BASE COUNT          754 a          520 c          542 g          802 t
ORIGIN      
        1 gtgtttgtcc acaccaaacc tgcctaccga agcgacggaa caaaaacagc agcggtaagg
       61 aagatgagaa ccgacagcaa caaggcatgg aagggagcgt taaagcgggc aggcattagc
      121 aacttccgct tccatgacct gaggcatacc tgggcaagct ggctggttca gtccggtgtc
      181 tctcttcttg cgcttaaaga gatgggagga tgggaaactc tcgaaatggt tcaaagatac
      241 gcccaccttt cagccgggca tcttaccgag cacgcgagca aaatcgatgc gattataagt
      301 cgcaatggca caaatacggc acaagaggag aacgtagttt acttaaatgc gaggtaactt
      361 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaattat
      421 aagaatccgc ttgtaattca aaggattatc ccatcaacac tgcgctcaca cgtcccacca
      481 catcaaaaca tgtaaagcct tgcaagccat tgtgaggcct tatgtgtctc agttttgtct
      541 catcagacat agcaagtatc gatcaattga gacttggatg atagacttca tgcctttcag
      601 agctcattga ttaaataaat gttaaagtta tttgtaaagt acacatctat aggtgtgctg
      661 aacacactta tacactgggt ggtttttggt gtatgtatct atgccgcaca taccagtcag
      721 gctctggcaa acttcacagg tttcgtagtg gctgtgagct ttagcttctt cgcgaatgca
      781 agattcacat tcaaagcatt gactacagcg atgcgctaca tgttatacgt gggatttatg
      841 ggaatattga gtgtgattgt tggttgggca gctgataaat gctcacttcc tccaatagtc
      901 actcttatca ccttctccgc catcagtctg gtgtgcggtt tcgtctattc aaagttcatt
      961 gtctttaggg atgcgaaatg aaaatatctc ttgtcgttcc tgtcttcaat gaagaagaag
     1021 cgatacctgt tttctataaa acggtacgtg aattccaaga gttgaagcca tatgaagtag
     1081 aaattgtatt cataaatgac ggaagtaaag atgccacaga gtcaattatt aacgcgctgg
     1141 ctgtttcaga cccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag
     1201 ccttatttgc agggttagac catgcaagcg gcgatgctgt aattcctatt gatgtcgacc
     1261 tgcaagaccc aattgaggtt atccctcatc ttattgaaaa gtggcaggca ggtgctgaca
     1321 tggtgcttgc taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg
     1381 agtggttcta taaattacac aacaaaataa gcaccccaaa gatcgaggaa aatgtcggag
     1441 attttcgact catgtctcgt gaggttgtgg agaacattaa actgttgcct gagcgcaatc
     1501 ttttcatgaa aggcatactg agctgggtgg gtggtccgac ggatgtcgtt gaatatgtac
     1561 gcgcagagcg tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac
     1621 tggaaggtat cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt
     1681 ttgttgcaag catttcattt ttatatggtg catggatgat tatagacacc cttgtctttg
     1741 gtaacccagt acgcgggtat ccctccctgc ttgtatcaat acttttcttg ggtggagtgc
     1801 aactgatcgg gattggtgtt ctcggagaat atataggtag aatctatatt gaagtaaaaa
     1861 atagacccaa atacatcata aaaaaatctc atcgaggtaa cccatgatta aaattaattt
     1921 atttaaaaat gcaaatctcc ttgccttcat atcatgtttt gcaatatcaa tttattgtta
     1981 ttgggggtgg ttgtacgatg gaacacttaa tattgatggc gagtttacaa ataattttta
     2041 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
     2101 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
     2161 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
     2221 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
     2281 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
     2341 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
     2401 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
     2461 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
     2521 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
     2581 gtacatctca aattatatac aaaatgcaag cgatatta
//
LOCUS       DBBBSB010000277         2560 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.284, whole genome shotgun sequence.
ACCESSION   DBBBSB010000277 DBBBSB010000000
VERSION     DBBBSB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.284"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            7..657
                     /locus_tag="ACIUS0_004365"
     CDS             7..657
                     /locus_tag="ACIUS0_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221834.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACIUS0_004366"
     CDS             629..1063
                     /locus_tag="ACIUS0_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGB0221835.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..>2560)
                     /locus_tag="ACIUS0_004367"
     CDS             complement(1836..>2560)
                     /locus_tag="ACIUS0_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGB0221836.1"
                     /translation="YDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILI
                     ISLLSFMLSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKI
                     GFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIF
                     IAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCIC
                     VYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT          776 a          481 c          531 g          772 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa
//
LOCUS       DBBBSB010000278         2532 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.285, whole genome shotgun sequence.
ACCESSION   DBBBSB010000278 DBBBSB010000000
VERSION     DBBBSB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.285"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..823)
                     /locus_tag="ACIUS0_004368"
     CDS             complement(<1..823)
                     /locus_tag="ACIUS0_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0221837.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1423..1671)
                     /locus_tag="ACIUS0_004369"
     CDS             complement(1423..1671)
                     /locus_tag="ACIUS0_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGB0221838.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1893..1988)
                     /locus_tag="ACIUS0_004370"
                     /pseudo
     CDS             complement(1893..1988)
                     /locus_tag="ACIUS0_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1985..2348)
                     /locus_tag="ACIUS0_004371"
                     /pseudo
     CDS             complement(1985..2348)
                     /locus_tag="ACIUS0_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            complement(2375..>2532)
                     /locus_tag="ACIUS0_004372"
     CDS             complement(2375..>2532)
                     /locus_tag="ACIUS0_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGB0221839.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          644 a          483 c          620 g          785 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg caatttttcc ttaaggtatg aaaaaaggag
     1081 ctgaagctat ataatagcta ttaatgccac tggtatcagt gaatcatgta tataaaaaac
     1141 tgtgttaagg aggctgtcca ttagcggttt tttattcttc tggaaccgcg gcatctttgc
     1201 gtatcttttt gtcttctcac cgtctggtcg gtgttttgct gagactgcta atttcctgtc
     1261 tttgctagtg ctgtactggt actgcccaat catgattggc gggggcgggc ggagttgaga
     1321 ccgcagacac gtcgtatgca agaacgtgct gcggctggct ggtgaacttc cgatagtgcg
     1381 agtattgaat gatttccagc cgttactgat tttacgtgct aattagtgaa caaaccactc
     1441 gtcagcagac tcccaggtat ctttcagagt ttcctgaaca aaagttttag ctgaatcttt
     1501 atcggcggtg cgcgtaacag aaaggccatc gttgctggtg gcttttacga tcacctctac
     1561 atcgtcataa cgtttactga tgcgtcgggt taattcttcc tttaacgcat ccacagcacc
     1621 ggttggcatt ttagtcattt tctctttggc tatgcagatt tcaaaacgca taaaaatccc
     1681 tctgcactgt gtttttatac agtattattt ttaactgtat ggataaacag tgtcaagagt
     1741 gagggaaagt gattttttgg ggcatgattg ggacactttg ttacatatga actttgccga
     1801 ttttcatatg acatggcaat ccaacgcttt gattattcta aagaaaacac atgctcttgg
     1861 gcgttcttta gtgattttta aaaatcccgt atcacgcagt taaagtggcg ggcatactct
     1921 tcaaggctgg tgatccccaa ccgcacccat ttaggatgtg accattgcgg tagaccgatg
     1981 taaatcataa cgcgtgaagg atctcttcca cgctacgcac acgggcgatg cgcgggtaga
     2041 tatgattgcg ggtagatatg attaatgctg ttattgtgtt gctcggcgct ggcggcacta
     2101 caggcgtctt cggcaatcac cagattaaag ccaagttccc aggcattgcg ggcggtggat
     2161 tcaacaccga tattggtcga gatcccacat aacactattg tatcgatacc tcggcggcgc
     2221 agttgcaact ccagatccgt accgtaaaac gcaccccact gacgtttgat gatttcgata
     2281 tcactgtcag ttgcacctaa tgcagctgga tgttgccacc agttttcggg caacacttta
     2341 gcgggggagt gcccctatta agttgtcaag catgttatga cccctgcggg gtataaaaag
     2401 tcccgtcgcg catcatggcg aacagaacgt cgcagcgtcg tctcgccagg gcgataagca
     2461 cctgattgtg tcgttttccc tgactcattt tgcgggtgta gtaagccctg gagagcggat
     2521 ccctgagcgc gg
//
LOCUS       DBBBSB010000279         2491 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.286, whole genome shotgun sequence.
ACCESSION   DBBBSB010000279 DBBBSB010000000
VERSION     DBBBSB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.286"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>2491
                     /locus_tag="ACIUS0_004373"
     CDS             <1..>2491
                     /locus_tag="ACIUS0_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGB0221840.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          581 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa t
//
LOCUS       DBBBSB010000280         2463 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.287, whole genome shotgun sequence.
ACCESSION   DBBBSB010000280 DBBBSB010000000
VERSION     DBBBSB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.287"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..171)
                     /locus_tag="ACIUS0_004374"
     CDS             complement(<1..171)
                     /locus_tag="ACIUS0_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGB0221841.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAG"
     gene            622..1812
                     /locus_tag="ACIUS0_004375"
     CDS             622..1812
                     /locus_tag="ACIUS0_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HGB0221842.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(2202..>2463)
                     /locus_tag="ACIUS0_004376"
     CDS             complement(2202..>2463)
                     /locus_tag="ACIUS0_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HGB0221843.1"
                     /translation="NMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQ
                     IEKIYPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          711 a          500 c          525 g          727 t
ORIGIN      
        1 accagcagcg aaaaagaata gtgggacgtg ataactaaaa acaaaaggat acaatttgcc
       61 agcccccaac ccaaggtgac ctaaatatat agcaaaaata ccgagaaatt ttaaagcatc
      121 aacccaatca tgtcttattt ttctgttgct gtcgattttg ttattataca tttaaagtag
      181 gctctccagt tttgtgtgat ttttaaacac tcattgcaaa tatgaatgcc tgaaagttta
      241 tgattattaa tcaacgagtt caatattaat ttattatttt tcaaagtcgc actatatagg
      301 catttacttc cgcagtacca ccgatagtat cgatatcaaa tcagcctgcg gccgcgataa
      361 ctttcgtaac attctgccag cggctttgtt gaataaatca ggttcaagat aatcgtctct
      421 gcatcaggtt agagtttcag gcaatacatc ctctttctgg catacctgcc actctcgcac
      481 catggtcata gcctttacga tctgcatact caccagacca gagcccgctc ccttttaggc
      541 tgaggaatcc ccagaaaaga agacaggcat agagttttgt gatctactga ttgcgctaac
      601 aaattcaatg agatcacctc tatgcctgcc cgtaaagtat gccagacttt tttccgtaat
      661 gctttagctt ccacgcatca gtatcgacaa aatgccatta ttgattctgc tgctgcttta
      721 gtcggtggcg catctctttc tcttaccagc attggccggc atttacccgg tcctgctcgc
      781 gtgaaagata aaataaaacg cgttgactgt ctgttgggta ataaacgact tcataatgat
      841 atccctctga tattcaaaaa tattacatcc atgatgacaa ataaactgtc gtggtgtgtt
      901 atcgctgttg actggagcgg ttaccctttt caggaatatc atgtccttcg tgccagcctc
      961 ctctgcgacg gtcgctctat ccccctgatg agtcaggtct tcccgtcaaa aaaaaataac
     1021 gaggcggtgg aaatcgcttt tctggatgcg ctttccgggg ccatttctcc ccggactcgt
     1081 gttgttattg ttactgatgc tggttttcaa agtgcatggt tccgtcatat caaatcacag
     1141 ggatgggatt ttattggtcg tatcaggggg gtagtgaagt tcaggctgga tagtgacaaa
     1201 gataagtggc tggacataaa aatgtgcagg gggagttcag aggcaaaata cctggggacg
     1261 ggaaccctgg cccggaaaaa acgctcgcag tgtgaaggac atttttatct ttataaacat
     1321 tcacctaaag gcagaaaaag caggcgtgcc agagggaggc cgggcttacc aacaacggaa
     1381 aaagaacaaa aagccgcagg cagagaacca tggttaatat tcagcaatac agctgagttt
     1441 aatgccaaaa aaatcatgaa actgtacagt cgaagaatgc agatagagca aaactttcgg
     1501 gatgaaaaaa gtgaacgctt tggttttggc cttcgggcga gcagaagtcg gaggggagag
     1561 cgttttctgg tattaagtct actggttaca ctggccagta tcgtactctg gctacttggt
     1621 tatcattttg aaaataaagg atttcattta aagtatcagg ctaatagtct caaaaaacga
     1681 agagtattgt cttttctgac gctggctgaa aacgtcctga gatttaaccc tgaacttcta
     1741 cgacgagcac aacctgaaaa gatattgtgc cggttggcca gcacttaccg aagtatggtg
     1801 ctggcttact gatgaaaatt ttctggggat ccctcagcct ttcggggagg aatgagggct
     1861 ctgatattct tttgccgtag ttcatcgtga aaccgtcgga tgtcatttgg cctgagtgac
     1921 ccatgcctcc caaaagcaga gtcgctagtg ttatgtaatt aggcggttaa acttcaattc
     1981 tcagtgttaa gtttgggtaa acgcttgtaa atggtattgc taatttttta attgactaat
     2041 attagaaacg gttagtgtga ttgtatatag atatttgagt gtaaggtatt taaaggggca
     2101 ttcagcccct ccgtctattt gtgattgcat ccattgatgt gtgtattatt ttgagcttga
     2161 aaaagggagg cgctctttaa taataaataa atctatcaaa gctaaatatt aaatggaagc
     2221 cacccttttt tctctgatag ttttattatc accaaaccat ttatttcagc tatcgaatct
     2281 ggattcggcc aggaaggtaa actcttcaca attggataaa tcttctctat ttgttcttta
     2341 tttgctggtt tgcaattaca gattccatag taattcataa atgcaaccat cctaaaatag
     2401 ttgccaccat cccaccaaaa aaaagatttt ccaaaagtgt ctgcgctcat ggagagcata
     2461 ttt
//
LOCUS       DBBBSB010000281         2439 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.288, whole genome shotgun sequence.
ACCESSION   DBBBSB010000281 DBBBSB010000000
VERSION     DBBBSB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.288"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..565
                     /locus_tag="ACIUS0_004377"
     CDS             <1..565
                     /locus_tag="ACIUS0_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221844.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="ACIUS0_004378"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="ACIUS0_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGB0221845.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACIUS0_004379"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="ACIUS0_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGB0221846.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="ACIUS0_004380"
     CDS             2167..2403
                     /locus_tag="ACIUS0_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGB0221847.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          627 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DBBBSB010000282         2436 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.289, whole genome shotgun sequence.
ACCESSION   DBBBSB010000282 DBBBSB010000000
VERSION     DBBBSB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.289"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..360
                     /locus_tag="ACIUS0_004381"
     CDS             <1..360
                     /locus_tag="ACIUS0_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGB0221848.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACIUS0_004382"
     CDS             361..726
                     /locus_tag="ACIUS0_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGB0221849.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACIUS0_004383"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACIUS0_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACIUS0_004384"
     tRNA            1605..1680
                     /locus_tag="ACIUS0_004384"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACIUS0_004385"
     tRNA            1682..1758
                     /locus_tag="ACIUS0_004385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACIUS0_004386"
     tRNA            1761..1836
                     /locus_tag="ACIUS0_004386"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACIUS0_004387"
     tRNA            1840..1914
                     /locus_tag="ACIUS0_004387"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACIUS0_004388"
     CDS             2207..2422
                     /locus_tag="ACIUS0_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGB0221850.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          520 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcatta
//
LOCUS       DBBBSB010000283         2419 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.290, whole genome shotgun sequence.
ACCESSION   DBBBSB010000283 DBBBSB010000000
VERSION     DBBBSB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.290"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..244
                     /locus_tag="ACIUS0_004389"
     CDS             <1..244
                     /locus_tag="ACIUS0_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGB0221851.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(333..845)
                     /locus_tag="ACIUS0_004390"
     CDS             complement(333..845)
                     /locus_tag="ACIUS0_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGB0221852.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1040..1804
                     /locus_tag="ACIUS0_004391"
     CDS             1040..1804
                     /locus_tag="ACIUS0_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221853.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1755..>2419
                     /locus_tag="ACIUS0_004392"
     CDS             1755..>2419
                     /locus_tag="ACIUS0_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HGB0221854.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          682 a          539 c          614 g          584 t
ORIGIN      
        1 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
       61 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
      121 caccgtcgaa ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat
      181 cagcgaccag acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg
      241 gtagcggtaa ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga
      301 agctggattc gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta
      361 aaactaccaa cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact
      421 ttcactggat gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg
      481 ataaagcgcg aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt
      541 tctattgtat tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt
      601 ataccaagat ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca
      661 gcttcccatg gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat
      721 gctggtggca atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga
      781 agcccccata ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc
      841 atcaatgtct ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt
      901 aggcttaaac accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg
      961 caggagtgag tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta
     1021 aatcacagga agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg
     1081 ggccggagtc cttagcctcc gtgatatagg cgagaaatac ggcgttacag aaggggctat
     1141 caggaagagg gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg tttgcaaaaa
     1201 tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca
     1261 aaaaagtacg caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg
     1321 taccgatccc ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg
     1381 ttatgcccgt cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct
     1441 cgaggacgaa ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg
     1501 gtggttggtg tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa
     1561 tatcagcgcc gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg
     1621 cacgcttgcg acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga
     1681 taaggtgtct ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg
     1741 agagcgtgac ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac
     1801 gtagtttctg gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa
     1861 aatcatggga tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt
     1921 gttgtgcccg tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc
     1981 agatagacag gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc
     2041 gggttactgg ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt
     2101 cactggaggg gatcgatatg ttgtggttgg aagaagccca cgcactgacg gaataccagt
     2161 ggaaaattct ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc
     2221 ccggacttgt tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc
     2281 tcatccgcaa aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta
     2341 tcgacgcggc gcgacgccgt gatccggatg gttttaaaca tgtgtatgag ggcgttccgg
     2401 agtctgatga tgatgcggc
//
LOCUS       DBBBSB010000284         2325 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.291, whole genome shotgun sequence.
ACCESSION   DBBBSB010000284 DBBBSB010000000
VERSION     DBBBSB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.291"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACIUS0_004393"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACIUS0_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGB0221855.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACIUS0_004394"
     CDS             282..452
                     /locus_tag="ACIUS0_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGB0221856.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACIUS0_004395"
     CDS             527..802
                     /locus_tag="ACIUS0_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221857.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACIUS0_004396"
     CDS             902..>2325
                     /locus_tag="ACIUS0_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HGB0221858.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DBBBSB010000285         2310 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.292, whole genome shotgun sequence.
ACCESSION   DBBBSB010000285 DBBBSB010000000
VERSION     DBBBSB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.292"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(593..2197)
                     /locus_tag="ACIUS0_004397"
     CDS             complement(593..2197)
                     /locus_tag="ACIUS0_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0221859.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          598 a          555 c          509 g          648 t
ORIGIN      
        1 ctataagtta atctttaatt gtgtctttaa gttaacttta agttaagccc ctgtagggac
       61 tagagaccat aattaagatg attgtgtctt attgctattc acttatcgac tgacccagag
      121 cacacaggaa gcattcactt taagtagaac gcttggtgtc tgtaattggt catcccgccc
      181 ctgaaaggat gcgataagtt acggttagtt gtcctagaag agaaaacttt ttgtgtccct
      241 tctccctata gtgagtcgta ttaattatcc ttgacaagta gagaaaacca tgagtcaaaa
      301 acatgaccac acaggtctgt agtaagtgtc atcctagaag agaaaacttt ttgtgtccct
      361 tctccctata gtgagtcgta ttaattatcc cttgacaagc agagaaaact atgagtcaaa
      421 aacatgaccg cacaggtctg tagtaagtgt catcctagaa gagaaaactt tttgtgtccc
      481 ttctccctat agtgagtcgt attaattatc ccttgacaag tagagaaaac tatgagtcaa
      541 aaacatggcc acacaggtct gtagtaagtg tcatcctaga ctatggtctc ctctaggtct
      601 ccttataaac atcagccatc acattgacca gcctatcaac ctcgacctct ttagcataac
      661 ggtcgtacgt aatagaccct gaactatgcc ccatgatcgc ctgtgcatac gccaactcga
      721 ccccatgttg ttttaactgg gccgcaactg tatgtctaaa cgagtgaaac gctaagccgc
      781 tacggtctac gaggacttta ggcaacaacg tccgattaaa ccatttactc acctgctcac
      841 cgtaaccgtt cttcatgaga cgcagaccat cgaacaccat agcgtcatca ccgtcagcgc
      901 ctctacgggt ctctacaagc gccataaagt cagccaatat aaaaccatag gccccatcga
      961 ccagaggcac acagcggtca ctatgagcgt tcttgatgct cttaccgggt agactactat
     1021 cgtcttcatt gatatgaata taaacggttc ccgcctcagt ggtcctgacg tctttgacct
     1081 gtagttgggc tatttcattg agtctcgccc cagtgatagc agccaaggcg gtaacataat
     1141 aatgatacgg cttaccagag gtcttctgag agtatgcctt agctgcgacc agtagttgcc
     1201 ccacctgctc cgtagagaag gcgttacggg cccctgaggc tttacgctga gggactttaa
     1261 gctccagacc ctcagtcatg ttctttttaa tcaagtcatt gcgcacagcc cacctaaaga
     1321 cagcagccat cttaataaga tacttgttgt tgatggtaac gacatccaga caatctgtct
     1381 tatcttcacg gctcaacagg tcagcaagtg ggacatcctt gaagcgctgc ttacggttcc
     1441 taggtaactg ctggagcaca tcacggaccc taagcatgtc agccctgtta gcgtctgcat
     1501 tgagacccag atggtcgaat atttcgatta acgcagcgtg tgacgccttg ttttccctta
     1561 aggttgcagg cttccagttc tgatagttct ccgcttcgta ttgttcaaag aggctcgcta
     1621 tggtaatgct aggcgtaact tctggtttta cttctggagc attaacagat agggacacag
     1681 agtctgcttg gttatcttgc ctatcaatat cattaaaaag atcgacgtaa tctatcagag
     1741 gctgactgtc tcccgcttcg atacgcctca tacaggcttt tagcaccttc agggcctcat
     1801 tgatgtaacg atgctggtca atgcttagcg gctcactagc cagagcatcg gtaaggctct
     1861 caccaagttc tccgtattgg tcccggtaga tatccctcat gtcaggctca aagagatctg
     1921 aacggcccat agacagttca cactcagcga tgtccttaag gtgttccctc atttcctcat
     1981 aggtagcgtt gggtctgtct gcgtgtattg ccttgagtgc tgcctttatg tgccttgagt
     2041 aagccatagc ggtgcgtcta tccttggtcc gtaatgatat tgaggtcatt ctatcattgt
     2101 ttgattgtct gaccctaaga cggaagtagt agacgccgtt acgctggtag agatagtaga
     2161 ctgctggaag tttgtgacta ctggatagct gaatcatatg tctgcatgcc ctgagagggt
     2221 tacacagtgg gttacagtaa gtaactctgc aaggaggctt aaagccgtat cccttaggat
     2281 tcagtaggtt ggagtacttt aagaaaggtc
//
LOCUS       DBBBSB010000286         2250 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.293, whole genome shotgun sequence.
ACCESSION   DBBBSB010000286 DBBBSB010000000
VERSION     DBBBSB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.293"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..242)
                     /locus_tag="ACIUS0_004398"
     CDS             complement(<1..242)
                     /locus_tag="ACIUS0_004398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221860.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACIUS0_004399"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACIUS0_004399"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGB0221861.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DBBBSB010000287         2247 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.294, whole genome shotgun sequence.
ACCESSION   DBBBSB010000287 DBBBSB010000000
VERSION     DBBBSB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.294"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..305
                     /locus_tag="ACIUS0_004400"
     CDS             <1..305
                     /locus_tag="ACIUS0_004400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221862.1"
                     /translation="VAKAVHPQCFCWRVLFSTVLLPGGGHFIIFLIVTGEKAEEKASS
                     SPLNNSMIPVHAKSDFQLTHTERCTKNRQPRQLRVCSRGLLRIFCKLSEEIFTTPE"
     gene            complement(491..565)
                     /locus_tag="ACIUS0_004401"
     tRNA            complement(491..565)
                     /locus_tag="ACIUS0_004401"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(530..532),aa:Gly,seq:tcc)
     gene            complement(569..644)
                     /locus_tag="ACIUS0_004402"
     tRNA            complement(569..644)
                     /locus_tag="ACIUS0_004402"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Thr,seq:tgt)
     gene            complement(647..723)
                     /locus_tag="ACIUS0_004403"
     tRNA            complement(647..723)
                     /locus_tag="ACIUS0_004403"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(687..689),aa:Arg,seq:tct)
     gene            complement(725..800)
                     /locus_tag="ACIUS0_004404"
     tRNA            complement(725..800)
                     /locus_tag="ACIUS0_004404"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Ile,seq:cat)
     gene            complement(977..1666)
                     /locus_tag="ACIUS0_004405"
     CDS             complement(977..1666)
                     /locus_tag="ACIUS0_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGB0221863.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1663..2022)
                     /locus_tag="ACIUS0_004406"
     CDS             complement(1663..2022)
                     /locus_tag="ACIUS0_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGB0221864.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(2022..2210)
                     /locus_tag="ACIUS0_004407"
     CDS             complement(2022..2210)
                     /locus_tag="ACIUS0_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221865.1"
                     /translation="MPFYFRKECPLNSGYLNMVPALRELMQAKHRAQKAVDELVDCVA
                     ELETRVGKFEKLVQEVLR"
BASE COUNT          573 a          609 c          487 g          578 t
ORIGIN      
        1 gagtagcgaa agctgttcat ccccaatgtt tttgctggcg ggtccttttt tctacggtcc
       61 tcctccccgg aggggggcat ttcattattt ttctaatcgt tactggtgag aaggcggaag
      121 aaaaagccag ttcttcgccc ctaaataaca gcatgattcc agtccatgca aaatctgatt
      181 tccagctcac acataccgag cgctgtacaa aaaaccgcca gccaaggcag ttaagagtgt
      241 gttcccgggg tttgcttaga atattttgta agttgtccga agagatattt acaacaccag
      301 aatgatgaat catcagtccc ctgccagagg ttcactacgt aacctgaccg acaatgcacc
      361 attctggtgc tgaaaaaaga gcactggaac tgcaacaaaa caacatcaca gaacaggaga
      421 tggtaaggag tcagacattc ccacacaata ttgtgtcagt gcaaataaca accttcgtct
      481 caaatctcgc tggagcgggc agcgggaatc gaacccgcat catcagcttg gaaggctgag
      541 gtaatagcca ttatacgatg cccgcatatg gtgccgacta ccggaatcga actggtgacc
      601 tactgattac aagtcagttg ctctgcctgc tgagctaagt cggcgctggc ccaccaccga
      661 ggactcgaac ctcggaccgt caacttagaa ggttgatgct ctatccggat gagctagtgg
      721 tggttggtgg cccttgctgg acttgaacca gcgacctggc gattatgagt cgctcgctct
      781 caccactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
      841 tcatctgacc accagcacgt ttaacgtcct gtgccatttt tcaggcataa aaaaaccgct
      901 cggcggcggg ttttgcttac tttaccatcg cgtacaaaat cggcaaagta tcagatttgc
      961 atgagatata tgccttttaa tctacttttg caatactttg ctttgaaaat gccgcttttt
     1021 gttctgaacg tgttctcatc acaaacaata aagcctcact atccagtcgg tgaaaaatgt
     1081 gtttcattac aacccagtga ccagtaaatg ttttggacca gtttttggtt gttactcccg
     1141 ccagtaatgc cagctcctga tattcataac cttccccacc aaaaagttct gcttttaccg
     1201 cctgcgccgc cagccagatt aattttttca ggcgttcctg tgttttctct gcaattttcc
     1261 tagtgccaga atgagcctta aactcttccc acgcccagtg ggttatggtg atctgatact
     1321 cccagcaaat acttccgctg taacaccaca acaaccaggc tttatagtgc tcttcaagag
     1381 acagaacagc ccgccgccac gatgatgtcg aaaacgcaac ctgactgacc agaggaattg
     1441 acgccccctt cgccagtgat tgttttcccg ggattggtgg attatcccgc gttatcattt
     1501 ttccggtcac ctcatcgcgg taccggattt ttttacgcct gtaacgccct gtatcgaaca
     1561 tggcattctc ctgccaggct tcaagctgac cttttgtcgc cccactcaaa tcagcggtgg
     1621 cgataatgag ctgctcacga acaaactgta aatactggtt attcatgcac actccagctc
     1681 tgtgattttt atccccaacc gaccaccagg aacaggcagt ccgcgcacaa tattggtttc
     1741 atcaaactgc tcgtcgtcga taagcaaccc cgcatgtgtc agtgcatcca gtggtgcttt
     1801 caggatattg tccaggtcac gacgccgctt atccggtggc tctgcaataa tctttatcgc
     1861 cagtcttccg gacaggctta atttcagtcg ctgctgacgg acaataagcg ccacatcccg
     1921 gcgataacgc tcaccggctt ttgatacaaa atatgtgctg ccacgacgtc gccagtaagt
     1981 attcaccgtc ggcggataag gtaaaataaa ctcatggcgc atcagcgcag cacctcctgc
     2041 accagctttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     2101 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     2161 ttgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat cagaaggagc
     2221 caataatgag cagaaaaacc caacgtt
//
LOCUS       DBBBSB010000288         2227 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.295, whole genome shotgun sequence.
ACCESSION   DBBBSB010000288 DBBBSB010000000
VERSION     DBBBSB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.295"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(453..536)
                     /gene="tnpB"
                     /locus_tag="ACIUS0_004408"
                     /pseudo
     CDS             complement(453..536)
                     /gene="tnpB"
                     /locus_tag="ACIUS0_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            534..664
                     /locus_tag="ACIUS0_004409"
                     /pseudo
     CDS             534..664
                     /locus_tag="ACIUS0_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_075985013.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            773..1039
                     /gene="ospE1"
                     /locus_tag="ACIUS0_004410"
     CDS             773..1039
                     /gene="ospE1"
                     /locus_tag="ACIUS0_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HGB0221866.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1429..>2227
                     /gene="ipaH1.4"
                     /locus_tag="ACIUS0_004411"
     CDS             1429..>2227
                     /gene="ipaH1.4"
                     /locus_tag="ACIUS0_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HGB0221867.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          735 a          476 c          384 g          632 t
ORIGIN      
        1 tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca gatgactgct
       61 tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaagct tcttaccata
      121 tttgaaataa agctcaatag cgcgaatttt ttgttcttca gtaaacatgt ttcatcttct
      181 gagagtccag gaaatcgtcc gcatccccat taccgtctta tatcactggc gctgacatgt
      241 gctcattgat gaagtgaatc tgaatgacat gttttgccat atcaacgcca accggtgtat
      301 atttcatttg tggagtctcc agtctgagga cgctttatgc atcccgtatt gggcagtatg
      361 atgccggaaa gctgcgaggc tccacacctg ccagtttcac ccttttaacc atcatactac
      421 tccgtgttta attgtgtggg atgcgttcat tacattttaa cagtgtcacc ccgtcggcca
      481 cgaaagataa acaggtgacc agaaaaggga tcttcatcca gcacatgcta tatcttgaac
      541 gtaacaacct gaccctccgc acccgcatca agcggctggc acgtaaaaca atctgcttct
      601 cgcgttccgt ggaaatccac gaaaagtcat cggctccttc attgaaaaac acatattcta
      661 ctgattggaa gcgccacccg aaaaagtatc gtttttttac agtaaacttc atttagccga
      721 ctataatgta aaaaaataaa ttttttacca aaatataaca taaggaagta atatgcttac
      781 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      841 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      901 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      961 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
     1021 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaaa
     1081 tatatcaggg tttgttacta ataacttctg aaaacagaaa aaaatagagc agtaattcta
     1141 tttcagagag gagtaactcc atctatcctc gatacgtact gaacgttatc gtgatatatt
     1201 catcatgctt tcttcaccat tattcgagta tagggagaga cgcaatgacc ttaaatacct
     1261 ttttatggct gaattaccat tctcaggaat aacagcagac tccttcccat agacggctta
     1321 gggggaaagc aatacccgga gaaagggtac aaagcaaact gttttaaaat taataagcca
     1381 tattctattt aactagaatt cttatcgttg ataagaattc tagttataat gattaaatca
     1441 accaatatac aggcaatcgg ttctggcatt atgcatcaaa taaacaatgt atactcgtta
     1501 actccattat ctttacctat ggaactgact ccatcttgta atgaatttta tttaaaaacc
     1561 tggagcgaat gggaaaagaa cggtacccca ggcgagcaac gcaatatcgc cttcaatagg
     1621 ctgaaaatat gtttacaaaa tcaagaggca gaattaaatt tatctgagtt agatttaaaa
     1681 acattaccag atttaccgcc tcagataaca acactggaaa taagaaaaaa cctattaaca
     1741 catctccctg atttaccacc aatgcttaag gtaatacatg ctcaatttaa tcaactggaa
     1801 agcttacctg ccttacccga gacgttagaa gagcttaatg cgggtgataa caagataaaa
     1861 gaattaccat ttcttcctga aaatctaact catttacggg ttcataataa ccgattgcat
     1921 attctgccac tattgccacc ggaactaaaa ttactggtag tttctggaaa cagattagac
     1981 agcattcccc cctttccaga taagcttgaa gggctggctc tggctaataa ttttatagaa
     2041 caactaccgg aattaccttt tagtatgaac agggctgtgc taatgaataa taatctgaca
     2101 acacttccgg aaagtgtcct gagattagct cagaatgcct tcgtaaatgt tgcaggtaat
     2161 ccattgtctg gccataccat gcgtacacta caacaaataa ccaccggacc agattattct
     2221 ggtcctc
//
LOCUS       DBBBSB010000289         2218 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.296, whole genome shotgun sequence.
ACCESSION   DBBBSB010000289 DBBBSB010000000
VERSION     DBBBSB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.296"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACIUS0_004412"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACIUS0_004412"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGB0221868.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACIUS0_004413"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACIUS0_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGB0221869.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACIUS0_004414"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACIUS0_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGB0221870.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DBBBSB010000290         2208 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.297, whole genome shotgun sequence.
ACCESSION   DBBBSB010000290 DBBBSB010000000
VERSION     DBBBSB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.297"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..124
                     /locus_tag="ACIUS0_004415"
     CDS             <1..124
                     /locus_tag="ACIUS0_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HGB0221871.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="ACIUS0_004416"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="ACIUS0_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HGB0221872.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            2131..>2208
                     /locus_tag="ACIUS0_004417"
     CDS             2131..>2208
                     /locus_tag="ACIUS0_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221873.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          679 a          400 c          376 g          753 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ctaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgat atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaagag cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga
     2101 tggtgatttt aaggtgcttg cgtagcttcc atttccatca gatgtccttc ctgctccgct
     2161 actgaaggcg tggtgcgtaa cggcaaaagc actgccggac atcagcgc
//
LOCUS       DBBBSB010000291         2183 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.299, whole genome shotgun sequence.
ACCESSION   DBBBSB010000291 DBBBSB010000000
VERSION     DBBBSB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.299"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..203
                     /locus_tag="ACIUS0_004418"
     CDS             <1..203
                     /locus_tag="ACIUS0_004418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221874.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACIUS0_004419"
     CDS             293..694
                     /locus_tag="ACIUS0_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221875.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACIUS0_004420"
     CDS             696..1301
                     /locus_tag="ACIUS0_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HGB0221876.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACIUS0_004421"
     CDS             1288..>2183
                     /locus_tag="ACIUS0_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221877.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DBBBSB010000292         2176 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.300, whole genome shotgun sequence.
ACCESSION   DBBBSB010000292 DBBBSB010000000
VERSION     DBBBSB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.300"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            82..390
                     /locus_tag="ACIUS0_004422"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACIUS0_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..>2176
                     /locus_tag="ACIUS0_004423"
     CDS             362..>2176
                     /locus_tag="ACIUS0_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGB0221878.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQR"
BASE COUNT          562 a          476 c          605 g          533 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaggt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgc
//
LOCUS       DBBBSB010000293         2169 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.301, whole genome shotgun sequence.
ACCESSION   DBBBSB010000293 DBBBSB010000000
VERSION     DBBBSB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.301"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            156..341
                     /locus_tag="ACIUS0_004424"
                     /pseudo
     CDS             156..341
                     /locus_tag="ACIUS0_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(441..647)
                     /locus_tag="ACIUS0_004425"
                     /pseudo
     CDS             complement(441..647)
                     /locus_tag="ACIUS0_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(700..951)
                     /locus_tag="ACIUS0_004426"
     CDS             complement(700..951)
                     /locus_tag="ACIUS0_004426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221879.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            1403..1687
                     /locus_tag="ACIUS0_004427"
     CDS             1403..1687
                     /locus_tag="ACIUS0_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGB0221880.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1687..1962
                     /locus_tag="ACIUS0_004428"
     CDS             1687..1962
                     /locus_tag="ACIUS0_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGB0221881.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          602 a          538 c          521 g          508 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg ggatatatcg
      421 cagctctgac gactcactcc ttatttaccc gggaaatact tctccagcaa tgcatacagg
      481 atggcagcgg tttcagcatc cattatgccg tcataatttt tctgccggaa atgaagctga
      541 aaagcgcgga caagcgcctt atatccgtca tccgtctcca caccagaaat actgtatcca
      601 taggtcttca gccgtgacac gatatcaacc ttatccggaa tgccctgcga ttcaaaaaat
      661 aaatatttag tcagtaattt tatgtttttc ctgaaaaaat tactcttttt cgaactccag
      721 tgagcgttca cgactcatca gtgtgaaaat gatggcaaaa acagcagaca gtatcagtac
      781 gataaatgtg gccccccatc ccagataatc gacaacaagt cccattccca gtgtcgcaac
      841 aattgagccg aagatatagg aaaatgtgcc tgacagccct aacagaataa gtttacgcgt
      901 aaatcgccct gaatccagaa gagcattaag tatcacataa acaggttcca taccagtcct
      961 gaccgcatca ggacgccagt aaataaaatc tcgtagtcca ccggcatcag agacaagagt
     1021 aatcgtcgtt accggtattt cgactggttt gtactggtgt gagcggacaa aactactgta
     1081 cgtctctcat acacgctcgt aagcggccct cacggcccgc taacgcggag atacgccccg
     1141 actgcgggta aacccttgtc gggaccactc cgaccgcgca cagaggcatt ttcatcgctg
     1201 acaggccgca tggcctcgca gagccggagg gggactggtg atattcggac gaccgtaccg
     1261 gcttgcgccg ggcttcggcg gttttacacc tgtgctacat gtaaccaccg tatgccgcct
     1321 tatatgcgct gactcgcatt atttgccttg ttccgtctga aagtaatcac tatgattaaa
     1381 tatgattaac aactaatcgg gtatgcaaat gaaaaacaat accgcacaag caacaaaagt
     1441 aattaccgcg catgtgccat tacctatggc tgataaagtc gaccagatgg ccgccagact
     1501 ggaacgctcc cggggctggg ttatcaaaca ggcgctttct gcatggcttg cccaagagga
     1561 ggagcgtaat cgcctgacgc tggaagccct ggacgatgtg acatccggac aggttatcga
     1621 ccatcaggct gtacagtcct gggcggacag cctcagtact gaccatctgt taccggtgcc
     1681 acgctgatgg aactgaagtg gaccagtaag gcactttctg atttggcgcg gttatatgat
     1741 tttctggtgc tggccagtaa acctgctgcc gccagaacgg tgcagtccct gacacaggcg
     1801 ccggtcattc tgttaactca tccacgtatg ggagaacagt tgtttcagtt tgaacccagg
     1861 gaggtcagac ggatttttac tggcgagtac gaaatccgtt acgaacttac tggccagact
     1921 atttatgtat tgcgcctgtg gcacacacga gagaacaggt agattgggtg tggaggcaac
     1981 tttaacatag cctgcaggta ttaattctgg agcattgtta tcaggccaga tattttgcat
     2041 tccattaaat aatcttgtag gagataaaat aaattacgat gaaataacga aaattaccgc
     2101 tcgtgactgg cggcaatatc gcgctccggg ctggcaaata acccatcaga aacgttattg
     2161 ccagacatt
//
LOCUS       DBBBSB010000294         2157 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.302, whole genome shotgun sequence.
ACCESSION   DBBBSB010000294 DBBBSB010000000
VERSION     DBBBSB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.302"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(91..357)
                     /gene="ospE2"
                     /locus_tag="ACIUS0_004429"
     CDS             complement(91..357)
                     /gene="ospE2"
                     /locus_tag="ACIUS0_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HGB0221882.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(539..940)
                     /locus_tag="ACIUS0_004430"
                     /pseudo
     CDS             complement(539..940)
                     /locus_tag="ACIUS0_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1369..2157
                     /gene="virF"
                     /locus_tag="ACIUS0_004431"
     CDS             1369..2157
                     /gene="virF"
                     /locus_tag="ACIUS0_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HGB0221883.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          663 a          382 c          398 g          714 t
ORIGIN      
        1 tgctctactt tttctgtttc cagaaattat tagtaacaaa ccctgatata ttcttttgaa
       61 aatggagctt aaacgagtct gaaattaact tcagaaatca gaataccgtt ggctttgttt
      121 ttcatcaatt ccggcttcat ttatctttgt caaccattgc atttctgtag cagtgaattt
      181 caacgttttc gtccggttgg cagcaggtat ttgtaattcg tatatagctg cttttttatt
      241 gaaaacagta tatccatctg tagtaacagt agaagataat aatacaggat ttgatacttc
      301 aaggatgata ttttcttgtt tttgtggaag gcaagggaat atagtttgtg taagcatatt
      361 gcttccttat gttatatttt agtaaagaat ttatttttta cattatagtc ggctaaatga
      421 agtttactgt aaaaaaacga tacttttttc gtaagcgatg agatgtggag ccagaaaaat
      481 ggctgcgtta cgtcattgaa ctgtccttga cccacgatat tcagacagtc gttgtatctt
      541 aagttgacat tacccgctga cgtcgatatt cccttggtga gagataaccc aacgcactat
      601 gcggatggtt ttcattgtaa tgctcgatcg ccactgcaag gttatgcaat gccgttcttg
      661 gtttcggcat gaacgcgatg cagtcttcct tcatcgtttt catgaaccgt tctgctattc
      721 cattactctg agggctactg actgccgtcg tgcagggctc cagattcaac tctctggcga
      781 actgccgtgt ttcatgagcc gtataagccg aaccgttatc ggccactgga ttggccgttc
      841 cggtccttta tcgccatatc gctcctgaca tgacatcctg caccgttgct ttgtcataat
      901 ctcccgtgcc tgctgcccag tctatggctt cacgatcgca atatggaacg taacgctgtc
      961 atcgggagaa tcgtcaatgc ggcattcggt taacgcttac caatatcaac accaaccgga
     1021 tttgtggagc ctccagtctg aaggggcttt atgcgttccg tataggatat tatgatgctg
     1081 gagttttgcg aagctccact cctttccgaa catttacaca gatattgcct aagaaaagtg
     1141 aaaccataat atggttatag tccctttcag tgcaaatact tagcttgttg cacagagaaa
     1201 tagaagctgc ataagctctt tcttcaaaaa atgtaaataa agttaaatat aggaaaaatt
     1261 acttaatcta tcttaataac ggaaagattt tgtatacaat cactgttaca gaaatttctt
     1321 agttactctg taaacactaa atatagtttg gtatattctg ttgaatttat gatggatatg
     1381 ggacataaaa acaaaataga tataaaggtt cgcttgcata actatattat tttatatgca
     1441 aaaaggtgtt caatgacggt tagctcaggc aatgaaactt tgactatcga tgaagggcaa
     1501 attgctttta tagagcgaaa tatacaaata aacgtctcca taaaaaaatc tgatagcatt
     1561 aatccatttg agattataag ccttgacaga aatttattat taagcattat tagaataatg
     1621 gaaccaattt attcatttca acactcctat tctgaggaga aaagggggtt aaacaaaaaa
     1681 atattcctcc tctctgagga ggaggtttct atcgatttgt tcaaatctat aaaagagatg
     1741 cctttcggca aaagaaagat ctatagttta gcttgccttt tatcagctgt ttctgatgag
     1801 gaagctttat atacttcgat atcgatagct tcttctctta gtttttctga tcagataagg
     1861 aagattgttg aaaaaaacat cgagaagaga tggcgtcttt ctgatatttc aaataacttg
     1921 aatttatcag aaatagctgt tagaaaacga ttggagagtg aaaaattaac atttcaacaa
     1981 atccttcttg atattcgcat gcatcatgca gcaaagcttt tattgaatag tcaaagctat
     2041 attaatgatg tatcaagact tatcggaata tcaagcccat cttattttat aaggaaattt
     2101 aatgaatatt atggtataac tccaaagaaa ttttacttat atcataaaaa attttaa
//
LOCUS       DBBBSB010000295         2125 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.303, whole genome shotgun sequence.
ACCESSION   DBBBSB010000295 DBBBSB010000000
VERSION     DBBBSB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.303"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..128)
                     /locus_tag="ACIUS0_004432"
     CDS             complement(<1..128)
                     /locus_tag="ACIUS0_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HGB0221884.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDETFSV"
     gene            complement(67..390)
                     /locus_tag="ACIUS0_004433"
                     /pseudo
     CDS             complement(67..390)
                     /locus_tag="ACIUS0_004433"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(387..920)
                     /locus_tag="ACIUS0_004434"
     CDS             complement(387..920)
                     /locus_tag="ACIUS0_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGB0221885.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(986..1615)
                     /locus_tag="ACIUS0_004435"
     CDS             complement(986..1615)
                     /locus_tag="ACIUS0_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221886.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1619..1834)
                     /locus_tag="ACIUS0_004436"
     CDS             complement(1619..1834)
                     /locus_tag="ACIUS0_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGB0221887.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVPGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          544 a          564 c          469 g          548 t
ORIGIN      
        1 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
       61 ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg
      121 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc
      181 cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat
      241 ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt tgtaggtaat
      301 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
      361 agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc taatgcgctt
      421 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
      481 ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc
      541 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
      601 gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg ctccacccat
      661 gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt cgacagcttc
      721 attcccggta tgacaggctt accatccacc attgtggcac cacgacagat ggtccatatg
      781 ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag aaactggtca
      841 agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc cgacaggccg
      901 tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa taacgagcca
      961 acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc aggctctttc
     1021 ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca tccatgtctg
     1081 caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg caacggccaa
     1141 tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga ttaataattt
     1201 tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg acgaactcac
     1261 cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc ggtgcagacg
     1321 caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg acgtactcag
     1381 gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa
     1441 acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct
     1501 catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt ttattaagct
     1561 cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc ttcattattt
     1621 actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat
     1681 aagtcagaag ccccagaagc agacttcccg gcacaccaat cgcagcccac tgtgatggcc
     1741 tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag gtgccgtagg
     1801 caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg
     1861 gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg
     1921 aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag ttaatcggaa
     1981 taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct gcacagcaat
     2041 tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc
     2101 atcatcgcag actgaaagac tctga
//
LOCUS       DBBBSB010000296         2092 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.304, whole genome shotgun sequence.
ACCESSION   DBBBSB010000296 DBBBSB010000000
VERSION     DBBBSB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.304"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACIUS0_004437"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACIUS0_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGB0221888.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACIUS0_004438"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACIUS0_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGB0221889.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DBBBSB010000297         2085 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.305, whole genome shotgun sequence.
ACCESSION   DBBBSB010000297 DBBBSB010000000
VERSION     DBBBSB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.305"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..797
                     /locus_tag="ACIUS0_004439"
                     /pseudo
     CDS             1..797
                     /locus_tag="ACIUS0_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            912..1253
                     /locus_tag="ACIUS0_004440"
                     /pseudo
     CDS             912..1253
                     /locus_tag="ACIUS0_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1236..2073
                     /locus_tag="ACIUS0_004441"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="ACIUS0_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          504 c          631 g          435 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcct ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DBBBSB010000298         2071 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.306, whole genome shotgun sequence.
ACCESSION   DBBBSB010000298 DBBBSB010000000
VERSION     DBBBSB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.306"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..259)
                     /locus_tag="ACIUS0_004442"
     CDS             complement(<1..259)
                     /locus_tag="ACIUS0_004442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221890.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1410)
                     /locus_tag="ACIUS0_004443"
                     /pseudo
     CDS             complement(1012..1410)
                     /locus_tag="ACIUS0_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1413..1779)
                     /locus_tag="ACIUS0_004444"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="ACIUS0_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="ACIUS0_004445"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="ACIUS0_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          471 a          582 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gccgcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DBBBSB010000299         2061 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.307, whole genome shotgun sequence.
ACCESSION   DBBBSB010000299 DBBBSB010000000
VERSION     DBBBSB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.307"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..2061
                     /gene="sat"
                     /locus_tag="ACIUS0_004446"
                     /pseudo
     CDS             1..2061
                     /gene="sat"
                     /locus_tag="ACIUS0_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          753 a          363 c          409 g          536 t
ORIGIN      
        1 ggagattcag ggggccagtc tactcatacg gcatgatgat ttataaaaat gataaaacat
       61 ttcgtaatct tgaaatattc ggagacagcg gctctggagc atacttatat gataacaaac
      121 tagaaaaatg ggtattagtc ggaacaaccc atggtattgc cagcgttaat ggtgaccaac
      181 tgacatggat aacaaaatac aatgataaac tggttagtga gttaaaagat acctatagtc
      241 ataaaataaa tctgaatggc aataatgtaa ccattaaaaa cacagatata acattacacc
      301 aaaacaatgc agataccact ggtactcaag aaaaaataac taaagacaaa gatattgtgt
      361 tcacaaatgg gggaaatgtt ctgtttaagg ataatttgga ttttggttgc ggtggtatta
      421 tctttgacga aggccatgaa tataacataa acggtcagag atttacattt aaaggagcag
      481 gaattgatat cggaaaagaa agcattgtaa actggaatgc attgtattcc agtgatgatg
      541 ttttacacaa aataggcccc ggtactctga atgttcaaaa aaaacagggg gcaaatataa
      601 agataggtga aggaaatgtt attcttaatg aagaaggaac atttaacaat atataccttg
      661 caagcggaaa tggtaaggta atactaaata aagataattc ccttggcaat gatcaatatg
      721 cggggatatt ttttactaaa cgtggtggca cgctagattt aaatggacac aatcagactt
      781 ttactagaat tgccgccact gacgatggaa caacaataac taactcagat acaaagaaag
      841 aagccgttct ggcaatcaat aacgaagact cctacatata tcatgggaac ataaatggca
      901 atataaaact aacgcacaat attaattctc aggataagaa aactaatgca aaattaattc
      961 tggatggtag tgtcaacaca aaaaatgatg ttgaagtcag taatgccagt cttaccatgt
     1021 aaggccatgc aacagagcat gcaatattca gaagcacagc gagtcattgc tccctggtat
     1081 ttctttgtgg aacggactgg gtcaccgttt tgaaagaaac agagagttca tataataaaa
     1141 agttcaattc tgattacaaa agtaataatc agcagacctc atttgatcag cctgactgga
     1201 aaaccggggt gtttaaattt gatacattac acctgaacaa tgctgacttt tcaatatcac
     1261 gcaatgccaa tgttgaagga aatatatcag caaataaatc agctatcaca atcggcgata
     1321 aaaatgctta cattgataat cttgcaggga aaaatattac taataatggt tttgacttca
     1381 aacaaactat cagtactaat ctatccatag gagaaactaa atttacaggt ggcatcactg
     1441 cacataacag ccaaatagcc ataggtgatc aagctgtagt tacacttaat ggtgcaacct
     1501 ttctgaataa tactcctata agtatagata aaggagcaaa agttatagca caaaattcca
     1561 tgttcacaac aaaaggtatt gatatctccg gtgaactgac tatgatggga atccctgaac
     1621 agaatagtaa aactgtaacg ccgggtctcc actacgctgc tgatggattc aggctgagtg
     1681 gtggaaatgc aaatttcatt gccagaaata tggcatctgt caccggaaat atttatgctg
     1741 atgatgcagc aaccattact ctgggacagc ctgaaactga aacaccgact atatcgtctg
     1801 cttatcaggc atgggcagag actcttttgt atggctttga taccgcctat cgaggcgcaa
     1861 taacagcccc caaagctaca gttagcatga ataatgcgat ctggcatcta aatagccagt
     1921 catcaattaa tcgtctagaa acaaaagaca gtatggtgcg ttttactggt gacaatggga
     1981 agtttacaac ccttacagtg gacaacctta ctatagatga cagtgcattt gtgctgcgtg
     2041 caaatctggc ccaagcagat c
//
LOCUS       DBBBSB010000300         2053 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.308, whole genome shotgun sequence.
ACCESSION   DBBBSB010000300 DBBBSB010000000
VERSION     DBBBSB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.308"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            79..297
                     /gene="tdcR"
                     /locus_tag="ACIUS0_004447"
     CDS             79..297
                     /gene="tdcR"
                     /locus_tag="ACIUS0_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HGB0221891.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            553..1093
                     /gene="yhaB"
                     /locus_tag="ACIUS0_004448"
                     /pseudo
     CDS             553..1093
                     /gene="yhaB"
                     /locus_tag="ACIUS0_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            1115..2053
                     /locus_tag="ACIUS0_004449"
                     /pseudo
     CDS             1115..2053
                     /locus_tag="ACIUS0_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          723 a          356 c          338 g          636 t
ORIGIN      
        1 gttaatacaa aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca
       61 aaatttgagt taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca
      121 gcgataaata ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta
      181 ttttatcaat attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac
      241 gtcaactttt tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt
      301 cttctcccct cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg
      361 ctggtcgccg cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat
      421 cagcgtagga cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt
      481 gatagtgcgt aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca
      541 aggcaggcat caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca
      601 atgcacgcag tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag
      661 tttgcagaaa ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactc
      721 cggagagtat tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc
      781 atgattccga atccaaccgg aaaatatgag tccattcatc atagatattt ctacaggaga
      841 acatgatgat tatatcatac aaacattaga tgtaggcact tttgcacctt ttggtgaaca
      901 atgtacttgc tcagccgtca ataaaaaaga gctggaatgt attaaggaga cgatttctaa
      961 gtattgcgca aagttcaccc aaaaagaagc catcttaaca ccccctaggc actttaataa
     1021 aacctgcatt acttcagatt gttggcaaat tctctttttt tcacccgatc attttaataa
     1081 tgatttttat tgattattta taaaggataa aataatgttc cctgtttcat caattggtaa
     1141 tgatattagt agtgatttag tccgtcgaaa aatgaatgac cttcctgaaa gccccatagg
     1201 aaataacctc gaagcactag caccaggtat agaaaaacta aaacagacct ctattcaaat
     1261 ggtcacttta cttaacgcgt tacaaccaag tggaaaatgc attatcactg gtgattttca
     1321 aaaagaatta gcgtacttac aaaatgtaat tctttataat gactcgtctc ttcgtctgga
     1381 ttttttaggt tataacaccc aaattattca acgatcggac aatacctgcg aacttaccat
     1441 taatgaaccg ttaaaaaact aggaaatatc tacaggcaat atcaatgtta attgcccatt
     1501 aaaagatatt tacaatgaaa tcaggaggtt gaacgtaatc tttagttgtg ggactggaga
     1561 tatagttgat ctatcctctc tggacttacg taatgtcgat ttagattatt atgatttcac
     1621 agataaacat atggctaata ctattctaaa tcattttaaa ttggattcaa caaattttac
     1681 taatgccaac atgtttcagg ttaattttgt tagttcaaca caaaacgcca caatctcctg
     1741 ggattattta ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga
     1801 aaaaatcaag tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt
     1861 aaaaattatg agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt
     1921 agaaaatgaa ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa
     1981 cagaattaaa ttaccagaga tgaaagaatt ctcatcaaaa aaaacatatg attacatcga
     2041 tacaatcatt gaa
//
LOCUS       DBBBSB010000301         2032 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.309, whole genome shotgun sequence.
ACCESSION   DBBBSB010000301 DBBBSB010000000
VERSION     DBBBSB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.309"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..362)
                     /locus_tag="ACIUS0_004450"
     CDS             complement(<1..362)
                     /locus_tag="ACIUS0_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0221892.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACIUS0_004451"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACIUS0_004451"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HGB0221893.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DBBBSB010000302         2029 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.310, whole genome shotgun sequence.
ACCESSION   DBBBSB010000302 DBBBSB010000000
VERSION     DBBBSB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.310"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1824)
                     /locus_tag="ACIUS0_004452"
     CDS             complement(<1..1824)
                     /locus_tag="ACIUS0_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGB0221894.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACIUS0_004453"
     CDS             complement(1932..>2029)
                     /locus_tag="ACIUS0_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0221895.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DBBBSB010000303         2011 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.311, whole genome shotgun sequence.
ACCESSION   DBBBSB010000303 DBBBSB010000000
VERSION     DBBBSB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.311"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..492)
                     /locus_tag="ACIUS0_004454"
     CDS             complement(<1..492)
                     /locus_tag="ACIUS0_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HGB0221896.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACIUS0_004455"
     CDS             complement(523..1215)
                     /gene="fimC"
                     /locus_tag="ACIUS0_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGB0221897.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACIUS0_004456"
     CDS             complement(1435..1977)
                     /gene="fimA"
                     /locus_tag="ACIUS0_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGB0221898.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          587 a          426 c          426 g          572 t
ORIGIN      
        1 agcgctactc aacaacgcta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc
       61 aaagttaatt gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg
      121 ctgggctaac tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt
      181 aaaacagggc attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac
      241 gatatcctgt gaaccaatga actcatcatt acgccagaga tccacccgat acacgcccgc
      301 aggttgatga ttacctctct caaagcgcga taaatcagca acggatgtgc cattctctaa
      361 taaaaatgcc gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag
      421 agatgaataa caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt
      481 gggtattttc ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc
      541 tctgaccggg gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac
      601 tcctgagggt aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt
      661 ttggctgccc acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt
      721 gatatagtta ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg
      781 taattgaatt ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt
      841 cctgccgttc aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag
      901 agactcacga tctgccgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct
      961 tttgggttca ctaacaaata gtggcggtgt aatgatgaat gacttttctt ttacaccgct
     1021 gctgttttca atccacgaat tgaccagaaa gagctcattg gtatggctat ttttaatcca
     1081 taccgcagtc tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc
     1141 tccggcagca tgggcgctgg ccgaaatgat taaataaaat atcatgagca tcaataactt
     1201 tattttagtc atcatagtta atacgccttg tgtattcctg acggcaaaaa taaggagtat
     1261 aaagaaatga ttgctttggc ttcttacaac acatcattcc cttttcattt aataaagcgg
     1321 catgatacaa agtgaacgtg gtttcgattt acattgccac cataccgctt cagacaaccg
     1381 gtacattact ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc
     1441 gtatctcata ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt
     1501 gcctttataa cgtgcagaaa aatgaagaac gttggtcccg gggatcaagt tctggttggt
     1561 tgagaagcta ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc
     1621 gacacccgtt gctgttgttg tatttgtact ggatacaatg gccaataaat tatcattgat
     1681 tgcatcagcc tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc
     1741 gttcaactga atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc
     1801 ggtacgatat tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc
     1861 agcattcacc agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact
     1921 acccgtagcg gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata
     1981 tattccttca atctcattta tcgactccac a
//
LOCUS       DBBBSB010000304         2003 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.312, whole genome shotgun sequence.
ACCESSION   DBBBSB010000304 DBBBSB010000000
VERSION     DBBBSB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.312"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            274..549
                     /locus_tag="ACIUS0_004457"
     CDS             274..549
                     /locus_tag="ACIUS0_004457"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HGB0221899.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            747..>2003
                     /locus_tag="ACIUS0_004458"
     CDS             747..>2003
                     /locus_tag="ACIUS0_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGB0221900.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVD"
BASE COUNT          522 a          396 c          459 g          626 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcatc agggctgtca ggcacatttt cctatatctt cggctcaatt gttgcgacac
     1981 tgggaatggg acttgttgtc gat
//
LOCUS       DBBBSB010000305         1976 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.313, whole genome shotgun sequence.
ACCESSION   DBBBSB010000305 DBBBSB010000000
VERSION     DBBBSB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.313"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..837)
                     /locus_tag="ACIUS0_004459"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="ACIUS0_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          534 a          465 c          456 g          521 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtaaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcag
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DBBBSB010000306         1969 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.314, whole genome shotgun sequence.
ACCESSION   DBBBSB010000306 DBBBSB010000000
VERSION     DBBBSB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.314"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACIUS0_004460"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACIUS0_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACIUS0_004461"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACIUS0_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACIUS0_004462"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACIUS0_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HGB0221901.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACIUS0_004463"
     CDS             1708..>1969
                     /locus_tag="ACIUS0_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGB0221902.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DBBBSB010000307         1953 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.315, whole genome shotgun sequence.
ACCESSION   DBBBSB010000307 DBBBSB010000000
VERSION     DBBBSB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.315"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..85
                     /locus_tag="ACIUS0_004464"
     CDS             <1..85
                     /locus_tag="ACIUS0_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221903.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(102..461)
                     /locus_tag="ACIUS0_004465"
                     /pseudo
     CDS             complement(102..461)
                     /locus_tag="ACIUS0_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(461..1381)
                     /locus_tag="ACIUS0_004466"
     CDS             complement(461..1381)
                     /locus_tag="ACIUS0_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGB0221904.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..>1953
                     /gene="pgrR"
                     /locus_tag="ACIUS0_004467"
     CDS             1519..>1953
                     /gene="pgrR"
                     /locus_tag="ACIUS0_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGB0221905.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          483 a          483 c          455 g          532 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ccccccacac
      121 acacccagca cgccaatgcg ttcagcatca acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccaacgagg ctacccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgacat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcctggct tgatgcccga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcat aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cacgaggtgt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggcct ccttcattgg agccagtggc ataatggcgt
      841 attcttcgct gctaatatcg ctggttcggg cgtttgagcc agcttcaatg tgtggcagag
      901 catcaataga tttcacattg ttttcccaac cattgcggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgacgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag cttttcggca taggttccgg ccgtttgctc tttaacgccc ccacctgggt
     1261 gagataccac aattgccgga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttcagttcct catggagttg tcggttcgtt ttgacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac cctaaatcat gattagacct atcatttaaa tgattaatag
     1501 attgtatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgcgca gcagcccgcc tgagcatggt gcagtcagct
     1621 ttaagccaga tagtgcgccg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgtacccac tgaagcgggc gagcatcttt tgtctgttct tggccctata
     1741 ttgcatgaca tagattctgc cctgacatcc ctgagcgatc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gcaaaaacga tattgttacc agccatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctta ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtc
//
LOCUS       DBBBSB010000308         1913 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.316, whole genome shotgun sequence.
ACCESSION   DBBBSB010000308 DBBBSB010000000
VERSION     DBBBSB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.316"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(53..1702)
                     /locus_tag="ACIUS0_004468"
     CDS             complement(53..1702)
                     /locus_tag="ACIUS0_004468"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGB0221906.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DBBBSB010000309         1849 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.317, whole genome shotgun sequence.
ACCESSION   DBBBSB010000309 DBBBSB010000000
VERSION     DBBBSB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.317"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACIUS0_004469"
     CDS             complement(<1..257)
                     /gene="ydfV"
                     /locus_tag="ACIUS0_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HGB0221907.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="ACIUS0_004470"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="ACIUS0_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HGB0221908.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="ACIUS0_004471"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="ACIUS0_004471"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HGB0221909.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            880..1035
                     /gene="hokD"
                     /locus_tag="ACIUS0_004472"
     CDS             880..1035
                     /gene="hokD"
                     /locus_tag="ACIUS0_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGB0221910.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1253..1504
                     /gene="rem"
                     /locus_tag="ACIUS0_004473"
     CDS             1253..1504
                     /gene="rem"
                     /locus_tag="ACIUS0_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGB0221911.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1571..1849
                     /locus_tag="ACIUS0_004474"
                     /pseudo
     CDS             1571..1849
                     /locus_tag="ACIUS0_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          507 a          410 c          467 g          465 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttgacaggag aagcaggcta tgaagcagca aaaggcgatg
      901 ttaatcgccc tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa
      961 gacctctgcg aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac
     1021 gaacctgagg agtaagagac ccggcgagtg agaaatccct cgccacctct gatgtggcag
     1081 gcatcctcaa cgcacccgca cttaacccgc ttcggcggtt ttttgttttt attttcaacg
     1141 catttgaagt tctggatggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga
     1201 taaaagggat ggaccccgaa caggggagtg ctacttatct ggaaggattc tgatgatgaa
     1261 catcgaagaa ctgcgtaaaa ttttttgtga agatggcctc tatgctgtgt gcgttgaaaa
     1321 tggaaatatt gttagtcatt accgcattgt gtgtttgcaa aaaaatgggg ctgcgttaat
     1381 taattttgtg gatgcccgag tgacggacgg atttatcttg cgcgacggtg agtttgtcac
     1441 ttcattacag gcattgaaag agatcggaat aaaagctggc ttttctgctt tttcagaaga
     1501 ataaactcat ctacaatctt gcgcggggct gaactcccgc tgagtaacac cgtgccaccg
     1561 gagaaaaccg atggcacgca acgcaaaata ttacaatcat gataattcga ccgttcttgc
     1621 ccacacgcac gagcggtatt ctcacgcatt taagtcagac tggtaccagc atccctcatg
     1681 cactgaagaa caggccgaat ggcttattca gtgttaccgc aggcgcggat gcgaggttaa
     1741 aaaagccctt agcctcgact accgtcacta gataatctcc gtcaggctcc cttactccga
     1801 acagccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaa
//
LOCUS       DBBBSB010000310         1836 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.318, whole genome shotgun sequence.
ACCESSION   DBBBSB010000310 DBBBSB010000000
VERSION     DBBBSB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.318"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1147
                     /locus_tag="ACIUS0_004475"
     CDS             <1..1147
                     /locus_tag="ACIUS0_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGB0221912.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
     gene            1286..1798
                     /locus_tag="ACIUS0_004476"
     CDS             1286..1798
                     /locus_tag="ACIUS0_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221913.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
BASE COUNT          561 a          344 c          457 g          474 t
ORIGIN      
        1 gtctataacc acgaaccgtt atgcatcagg ttatgccaca ctgactgaag cagtaagtgc
       61 acaggatgaa cggaacagaa aaagagataa gaatagtcac gatggctcta cgatatcctt
      121 atcccagccc cttggtaata tcgggaattt gaacttcaat accacgaggt ataattcttc
      181 ccggggcaca ggaaacacgc ggtctaccag cctgagttat agtacggtat ggagaggcat
      241 aacattctcc attaactggg caaaaaacga cttgctgaca tcgcataaat ggaaagtaga
      301 tcggaaatta agcgtgggta tttcagtgcc attatcgctg ggtgacgaaa atcaaattta
      361 tgcgtcttca cagatgagtc gttcgggaga gcagggtaat aactatcagg tttcattatc
      421 aggtcagaat agtggtggtg tatggtggga tgtagcgaca aatataacta acgctcatca
      481 gtcacaaccg aagagcacga tgaacattgt gcaagttggc aaaaatggat catatggtca
      541 gtttagtagc cattacagct ccagcgaaaa catgaaacag ctaggtgcaa atttaagcgg
      601 tgggatactt attacaaggg atggacttac atttggtcaa aatgtggatg ggacattagc
      661 ccttatagag gcacccggag caacgggggt taacgttaat ggctggccgg gactgagtac
      721 tgattttcgt gggtatgcaa tactgccagt gcagccatac cgaagagacg atgttattct
      781 ggatgaaaaa acgataggta agaattacga cttaccacaa actagtcaat tggttgtacc
      841 gacagctgga gccgttgtgc ctgcgacatt ggcagtgaag agcggggata aaggattagt
      901 aaccttaaaa cagaaagagg gtaaacccat ccccttcggc gctgtgatta gttatagcaa
      961 agacactgaa aatatggccg gcattgtagg tgaagatggt attgcttatg tttctggctt
     1021 gagtgcggaa ggcgagttta atgtgaaatg gggttattct aaagatcaat cctgtatcgc
     1081 aaaataccaa cttcctgcga agaaaagtgc atcagggtta taccagatag cggcgacatg
     1141 tttatagttt ctaaattaag aaactcataa tttatttttg tgaagatgtt tccattattt
     1201 ggtggaagca ttttcgcgat agcacgtaaa tgcaaagaaa gaaactgcat tttgcagtgt
     1261 aaatgaaaat catggagaaa atataatgaa gaaaacattg ttagcaatca tgctggcggg
     1321 gacagctttt gcttctcagg cgggaacttt agtaagccag ggtactgaag cgtcagctaa
     1381 tcttactttg accaagccta tcgttgttaa taataccatt caaccagtga aaggggtata
     1441 tagcggtacg ttaactgcat ggacgccttt ggcaacaggt attgttggcg catctgatgg
     1501 gcagagccac gactatgctg taactttccc ggatgatatt tatgcggaga gcagtacttc
     1561 agcggatgca gtaatttctg gtgacaataa cccggaccat aaactgaaag tttccctgac
     1621 aacgcttgag caggatcctc cttcagctgc ctccgaagag attggcggta agcgttatat
     1681 gatgctgaaa aatactggaa caggtggtgc ctatcgcgtc gtttcccata tgaaagaaca
     1741 ggttgttgag ccagactctt acaccatccg tacccaggct tatatctacg cagaataatg
     1801 cagatgatta gttaatttca tggcgggatt tgatat
//
LOCUS       DBBBSB010000311         1832 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.319, whole genome shotgun sequence.
ACCESSION   DBBBSB010000311 DBBBSB010000000
VERSION     DBBBSB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.319"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..180)
                     /locus_tag="ACIUS0_004477"
     CDS             complement(<1..180)
                     /locus_tag="ACIUS0_004477"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221914.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="ACIUS0_004478"
     CDS             complement(321..875)
                     /locus_tag="ACIUS0_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGB0221915.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="ACIUS0_004479"
     CDS             complement(872..1162)
                     /locus_tag="ACIUS0_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGB0221916.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="ACIUS0_004480"
     CDS             complement(1162..1761)
                     /locus_tag="ACIUS0_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGB0221917.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          421 g          470 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa ta
//
LOCUS       DBBBSB010000312         1814 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.320, whole genome shotgun sequence.
ACCESSION   DBBBSB010000312 DBBBSB010000000
VERSION     DBBBSB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.320"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..347
                     /locus_tag="ACIUS0_004481"
     CDS             <1..347
                     /locus_tag="ACIUS0_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221918.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
     gene            complement(386..763)
                     /locus_tag="ACIUS0_004482"
                     /pseudo
     CDS             complement(386..763)
                     /locus_tag="ACIUS0_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            748..1752
                     /locus_tag="ACIUS0_004483"
                     /pseudo
     CDS             748..1752
                     /locus_tag="ACIUS0_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
BASE COUNT          443 a          509 c          456 g          406 t
ORIGIN      
        1 tggtgccagg tgaatattcc agtggaaaca gtattcgtcc cagaggaata acaaaagttg
       61 gaaacagcga actgagacgt ctgctttacg aagccgcctg gtcttatcgt acacctgcaa
      121 aagttggagc atggcttata tattaccgac cggactctgt aacacaatat tccaaagata
      181 ttgcatggaa agctcaacaa cgattgtgtt ctcgttaccg aactctgaca gcaaaaggga
      241 aaaaatcaca agtagccatt acggcggtgg ctcgtgagtt gactggattt atgtgggata
      301 ttgcacttgc tgcccaatca tcattcagtc agcagaagca aaattaaacc ctgaagcagt
      361 cagacacgga atgagtgatc ctcgaaaaag ttttcttccc gagacagact gcacgaagcg
      421 ctctttctgc tgcattatta tctacctcag ccaggccatc atcacagaag ttgcacaacg
      481 cagcccagtg cttcctgata taccggaacg catctcccag acgacatttc ttcgataacg
      541 tgtgttcttt ctcctgcatc agcttataca gggaagtcag gagcggtcta ctctgcattt
      601 gcctgaccgc caggcgttca gacaccggta acccgcgtat atcgtgctct atggcgtaca
      661 gttcaccgat tagtttcaga gcttcttccg ccgtcgcgct tttagtactg atatatacat
      721 cgtggatttt gcgtcgcgca tgagcccagc atcctgcttc cgtccgtcgg cgcgcagctc
      781 tccaatccgg ataatatcac cgacaacgcc gaccggatca gtgatgagtg gaaggccttc
      841 gaccgcgcca tcgaaagtaa ccacggtgtt cctgaggctg ccgcacgcct gaaagaacac
      901 ctccgggact tccgtaacac acatgccgcc gcacagctgg gcgtttctgc tgtggcggcc
      961 cttcccggtg atcgcctctc tgccgcactg atggttaaat cacacggcac cgtcagtgtc
     1021 gacggtaaag tctctgatgc tgacctcact tacctggaag aggtggcaaa cagcaccggg
     1081 caggaggtgg acaaaagccg cctgacatca caggcctgtg cccgcacggc actgatcact
     1141 gatgtgggta ttgctctggc tacggaactg gaaacggccg ggcagaaatg gtctctgggc
     1201 ttccccccca aattccagcg cgtcgacctg ttcaactaca acgtgctggt cagaaattat
     1261 gacagcagcg ccttcaaagg cgaccgctac cacaacacga aaaatggcat caacgccgac
     1321 atcggtgcca gtacggacct ggatgacaac tggacgctgg gactggtcgc acagaacctg
     1381 atctcccgta gtattgagac aaaagaagtg aacggtatca cggaaacctt caggatccgc
     1441 ccgcaggtga cggccggtgt ctcctggcac aacgcgatgt tcaccactgc atttgatgtg
     1501 gatctgaccc cggccagcgg tttcacctcc gacagcaacc gtcagtttgc cgccattggc
     1561 acagaattta atgcctggaa atgggcacag ctgcgcgccg gttaccgtca gaatctggcc
     1621 ggtaacgacg gcagtgcatt cacggccggg gtggggatct caccgtttga tgtggttcac
     1681 cttgatgttg caggcctgat cggcacggac aacacttacg gtgcggtcgc acagttccag
     1741 ttcaccttct gagttccacc tgcaaaacag catgcacatt atcgctgcat gttgtttcca
     1801 tcatcaaaag tctg
//
LOCUS       DBBBSB010000313         1809 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.321, whole genome shotgun sequence.
ACCESSION   DBBBSB010000313 DBBBSB010000000
VERSION     DBBBSB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.321"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            173..1198
                     /locus_tag="ACIUS0_004484"
     CDS             173..1198
                     /locus_tag="ACIUS0_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGB0221919.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACIUS0_004485"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACIUS0_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DBBBSB010000314         1804 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.322, whole genome shotgun sequence.
ACCESSION   DBBBSB010000314 DBBBSB010000000
VERSION     DBBBSB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.322"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACIUS0_004486"
     CDS             complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="ACIUS0_004486"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGB0221920.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1317..1469)
                     /locus_tag="ACIUS0_004487"
     CDS             complement(1317..1469)
                     /locus_tag="ACIUS0_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221921.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1487..1678
                     /locus_tag="ACIUS0_004488"
     CDS             1487..1678
                     /locus_tag="ACIUS0_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGB0221922.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          567 a          408 c          369 g          460 t
ORIGIN      
        1 attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca gggatgcgta
       61 aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg cgaacatttg
      121 gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact accgctcgct
      181 gacgagttgc cagtacagcc atataattga caataaaaga gaataccaga taactggagg
      241 aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt tgatagctgt
      301 ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa ccatagcgca
      361 ttgctcccca catcatcagg ggcagcaata atgacaaggt ataattggtg ctaaaaattg
      421 tgcttttttc atttaacggc atgcataaca gcaacaataa agcacctaat gccagtagcc
      481 agagcgcgaa ctcttttttg gtgactttgg catcaacctg ctgttttaat tgcgaataat
      541 agctacgtaa ataaaatgga tttcgcacta cccgaatgat gaagtagcac agcgggacac
      601 cgatcagatt acccaccagt aaggcctgat aattgattaa agtcccgagg ttaaaaggca
      661 tgacaccgac cagattttct ctgctcgcca gtaatcctac aaatgcagca aactggaaaa
      721 gtatcagaaa cagcgttgca ggaaacacta tctgccagaa tatacgctgg gaaattaaac
      781 gggtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac
      841 agagaatgat aatgataata aaattagccg ttaatgagag cgtttcgtaa aaacccagct
      901 ctgcatattt gcggacaaaa atccctaagg tgatccctgg caatgccgcc cagctgaaaa
      961 acatcatcat gctgatcatc agtgccaaag gcagataaaa gagaaaaacc tcaccggaag
     1021 aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca
     1081 gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg
     1141 ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt
     1201 cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata tgaaagttcc
     1261 aattgatata tatcaaacaa aataaccctg attaatgaat tattacgttt atcatgttaa
     1321 ttcatcatta ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat
     1381 gtatccactg acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta
     1441 atgtaagtac cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac
     1501 tagtatgcga aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag
     1561 ctttatcttc ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca
     1621 gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg
     1681 gttaccttct ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt
     1741 tgggattatc aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgactg
     1801 atgt
//
LOCUS       DBBBSB010000315         1797 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.323, whole genome shotgun sequence.
ACCESSION   DBBBSB010000315 DBBBSB010000000
VERSION     DBBBSB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.323"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            311..505
                     /locus_tag="ACIUS0_004489"
     CDS             311..505
                     /locus_tag="ACIUS0_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221923.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACIUS0_004490"
     CDS             866..994
                     /locus_tag="ACIUS0_004490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221924.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACIUS0_004491"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACIUS0_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          545 a          395 c          379 g          478 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctt
//
LOCUS       DBBBSB010000316         1761 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.324, whole genome shotgun sequence.
ACCESSION   DBBBSB010000316 DBBBSB010000000
VERSION     DBBBSB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.324"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..265)
                     /locus_tag="ACIUS0_004492"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="ACIUS0_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="ACIUS0_004493"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="ACIUS0_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HGB0221925.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1165..1350)
                     /locus_tag="ACIUS0_004494"
     CDS             complement(1165..1350)
                     /locus_tag="ACIUS0_004494"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221926.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1375..1647)
                     /locus_tag="ACIUS0_004495"
     CDS             complement(1375..1647)
                     /locus_tag="ACIUS0_004495"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HGB0221927.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          435 c          319 g          505 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gccagtgttg ttgcagcctt gctgccaacg aagcgccact
     1321 gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca gatgttatcc
     1381 gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt tcagtgtcct
     1441 ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct ccttaacgcc
     1501 gggttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg agcgacaacg
     1561 gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg aatgacagcg
     1621 cggacaatga acagtaacgg tagccatgga aaaccttaaa taccagtaga ttaccagaaa
     1681 cttctgtcga ttgaagacat cacccgaagt ttgaattcat tcgaatactt tttacgaggt
     1741 tcttttcgcc agtcctgtaa t
//
LOCUS       DBBBSB010000317         1746 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.325, whole genome shotgun sequence.
ACCESSION   DBBBSB010000317 DBBBSB010000000
VERSION     DBBBSB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.325"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            453..1301
                     /locus_tag="ACIUS0_004496"
     CDS             453..1301
                     /locus_tag="ACIUS0_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGB0221928.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1318..1599
                     /locus_tag="ACIUS0_004497"
                     /pseudo
     CDS             1318..1599
                     /locus_tag="ACIUS0_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1635..>1746
                     /locus_tag="ACIUS0_004498"
     CDS             1635..>1746
                     /locus_tag="ACIUS0_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221929.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGK"
BASE COUNT          498 a          398 c          411 g          439 t
ORIGIN      
        1 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
       61 tgagaacttc agtaaagata ccaccaaagc ccggaagtgg tgaaataaaa ccgggcacaa
      121 cacgaaggcg catttccggt atccataaag agtcggtctt gtctgtgaaa tttaaatggt
      181 gggagtgcgc ttccggttgt aaataacgac attgctgtgt gaagtaccag ttggcggcat
      241 cggtttaatt gctggctgat gtccgccctt tttaaagtga attttgtgat gcggtgaatg
      301 cggctatgcg cacgcggcac agttaaaagt catgttagtc cttattggtt tgggtgggaa
      361 agccaactgt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcaaca
      421 aagttcactt cggtgatgaa aggaaagaga aaatgttgaa tgtagctatt gaaaaccaga
      481 acgggtggaa ttatagtgca cctgcacctc ataaaacggg tgccgggatt gctaccccga
      541 cgatgactac tgcgcataac cgcgctcagg cggttttttt atgcgtcaag cacagccaca
      601 ttcagattat ggtggggcgt gcagggcagc cgcaaggctg gccggtttcg gtagtcaccg
      661 gttgtagcaa ccctgtgcgt ctcaccaccc atgagattgc tacctctggt ggtgagtcat
      721 ttaaactgac tatcgaggct gccatcatgg ctactatcct taccctttct caccctgacg
      781 caaccatcga aaatggtcgc gctgtcacta cgtctgttgc ggttgcggag ttcttccgga
      841 aaatgcacaa aaacgttatt cagaagatcg aaactctcga atgctcgccg gagtttaacc
      901 ggcttaattt taagcccgtc acctataccg acgccaaagg cgaaaaacgc ccaatgtacc
      961 aaatcaccaa aaacggcttc gttttcctgg tgatgggctt cactggcaaa aaagccgccg
     1021 cattcaagga agcctacatt gctgagttcg accgcatgga ggccgaactg cgccagaata
     1081 acaccccacc tgcagacaag atgattccgg gtgatggtcg cactctggtt gttcacttcg
     1141 acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg gtctgtaccc
     1201 tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac cggggcgcaa
     1261 ttaaaaatat gactgtggaa caattacttt ctttaaaata aaaatggagc tacggatatg
     1321 aaccctatgc ttgattattg ttttacctct gcacctcagc agaccataac acacaccgga
     1381 atcaaaaatt tgttatggat aatcaaaaaa aacctccagt agaaaggtga tcccgattta
     1441 attccctgtg cttatggcct gtgtgacaga caagtaagcc ctgataaggc ttacactgct
     1501 gaacttattt acatgcacgg aaccatgatg aggaaaaaga aacgcaaata ttgtagtgaa
     1561 atctgcgccg aaaaagacca gatggcacac gaactttaat taactgacta ttcgaaactg
     1621 aatttatgcc agcaatggca gggattcgct caaccttaat taaggagaaa aacatgaaca
     1681 ccaattatga agccactgtt gcaactactg ataacattgt tcacgaggtt tacctggaag
     1741 gaaagc
//
LOCUS       DBBBSB010000318         1744 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.326, whole genome shotgun sequence.
ACCESSION   DBBBSB010000318 DBBBSB010000000
VERSION     DBBBSB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.326"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..353)
                     /locus_tag="ACIUS0_004499"
     CDS             complement(<1..353)
                     /locus_tag="ACIUS0_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGB0221930.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACIUS0_004500"
     CDS             complement(350..940)
                     /locus_tag="ACIUS0_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221931.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACIUS0_004501"
     CDS             complement(927..1298)
                     /locus_tag="ACIUS0_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGB0221932.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACIUS0_004502"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACIUS0_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGB0221933.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          392 a          527 c          408 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DBBBSB010000319         1729 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.327, whole genome shotgun sequence.
ACCESSION   DBBBSB010000319 DBBBSB010000000
VERSION     DBBBSB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.327"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACIUS0_004503"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="ACIUS0_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HGB0221934.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="ACIUS0_004504"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="ACIUS0_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            821..>1729
                     /locus_tag="ACIUS0_004505"
     CDS             821..>1729
                     /locus_tag="ACIUS0_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HGB0221935.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          446 a          400 c          429 g          454 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggattt
//
LOCUS       DBBBSB010000320         1718 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.328, whole genome shotgun sequence.
ACCESSION   DBBBSB010000320 DBBBSB010000000
VERSION     DBBBSB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.328"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(110..577)
                     /locus_tag="ACIUS0_004506"
     CDS             complement(110..577)
                     /locus_tag="ACIUS0_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGB0221936.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACIUS0_004507"
     CDS             complement(643..882)
                     /locus_tag="ACIUS0_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HGB0221937.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACIUS0_004508"
     CDS             complement(1017..1622)
                     /locus_tag="ACIUS0_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0221938.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaa
//
LOCUS       DBBBSB010000321         1703 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.329, whole genome shotgun sequence.
ACCESSION   DBBBSB010000321 DBBBSB010000000
VERSION     DBBBSB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.329"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..83)
                     /locus_tag="ACIUS0_004509"
     CDS             complement(<1..83)
                     /locus_tag="ACIUS0_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGB0221939.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQW"
     gene            complement(241..582)
                     /locus_tag="ACIUS0_004510"
     CDS             complement(241..582)
                     /locus_tag="ACIUS0_004510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221940.1"
                     /translation="MHHSGVVNISSDNLQNILSKPREHTLNCLGWRFFVQRSVCVSWK
                     SDFAWTGIMLLFRGEELAFSSAFSPVTIRKIMKCPSGEEDRRKKDPPAKTLGMNSFRY
                     SDCWRVKFLMN"
     gene            complement(763..837)
                     /locus_tag="ACIUS0_004511"
     tRNA            complement(763..837)
                     /locus_tag="ACIUS0_004511"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(802..804),aa:Gly,seq:tcc)
     gene            complement(841..915)
                     /locus_tag="ACIUS0_004512"
     tRNA            complement(841..915)
                     /locus_tag="ACIUS0_004512"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(880..882),aa:Thr,seq:tgt)
     gene            complement(918..994)
                     /locus_tag="ACIUS0_004513"
     tRNA            complement(918..994)
                     /locus_tag="ACIUS0_004513"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(958..960),aa:Arg,seq:tct)
     gene            complement(996..1072)
                     /locus_tag="ACIUS0_004514"
     tRNA            complement(996..1072)
                     /locus_tag="ACIUS0_004514"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1262..>1703)
                     /locus_tag="ACIUS0_004515"
     CDS             complement(1262..>1703)
                     /locus_tag="ACIUS0_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGB0221941.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          466 a          449 c          349 g          439 t
ORIGIN      
        1 aaccactgca aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt
       61 gatatttttt ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga
      121 aaaaaggcca tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa
      181 aacacgcacg actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc
      241 ttaattcatg aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa
      301 tgtttttgct ggcgggtcct tttttctacg gtcctcctcc ccggaggggc atttcattat
      361 ttttctaatc gttactggtg agaaggcgga agaaaaagcc agttcttcgc ccctaaataa
      421 cagcatgatt ccagtccatg caaaatctga tttccagctc acacataccg agcgctgtac
      481 aaaaaaccgc cagccaaggc agttaagagt gtgttcccgg ggtttgctta gaatattttg
      541 taagttgtcc gaagagatat ttacaacacc agaatgatgc attatcagtc ccctgccaga
      601 ggttcactac gtaacctgac cgacaatgca ccattctggt gctgaaaaaa gagcactgga
      661 actgcaacaa aacaacatca cagaacagga gatggtaagg agtcagacat tcccacacaa
      721 tattgtgtca gtgcaaataa caaccttcgt ctcaaatctc gctggagcgg gcagcgggaa
      781 tcgaacccgc atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcata
      841 tggtgcgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctgcctg
      901 ctgagctaag tcggcgctgg cccaccaccg aggactcgaa cctcgcaccg tcaacttaga
      961 aggttgatgc tctatccgga tgagctagtg gtggttggtg gcccttgctg gacttgaacc
     1021 agcgacctgg cgattatgag tcgctcgctc tgaccaactg agctaaaggg ccggaggcag
     1081 aataataacc atatgtcatc acatctgcaa actcatctga tcaccagcac gtttaacgtc
     1141 ctgtgccgtt tttcaggtat aaaaaaaacc gcataaagcg ggctctttca aatgtccatg
     1201 tctgctattc gcctcgcggt acagctttgc gaagcgtaca agaattgaag cagtttttac
     1261 gtcaaaaagc aataactttt ttctctatac caaaagccat aaccattggt ttgtacaaaa
     1321 taaattctgc cacctttagc caatgctcaa tgcgtctttc acaggttctt aaactccatt
     1381 tcgggtgtgc atcattcagc agttcagcca ttttgcattt ggtcatcccc cgcccctcat
     1441 accgttgccg aaggacgcta atcaatcctg gatgctctgc cagcacctca cttatgaccc
     1501 gatcaataca taacgcctct gcatcagtac aatgcgccag ccagctcttt tgcttaccgt
     1561 taatcatatc ccgcaaaaaa gcctcaagtt cagacttgtt cagacctgct tttttcatcc
     1621 tccggagcgc ctcgttaatt gccgtttttg tcagcttttt agaggtcaac atcaggttaa
     1681 acatattccc cgtcttaccg cca
//
LOCUS       DBBBSB010000322         1685 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.330, whole genome shotgun sequence.
ACCESSION   DBBBSB010000322 DBBBSB010000000
VERSION     DBBBSB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.330"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..323)
                     /locus_tag="ACIUS0_004516"
     CDS             complement(<1..323)
                     /locus_tag="ACIUS0_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGB0221942.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPE"
     gene            complement(388..1338)
                     /gene="virK"
                     /locus_tag="ACIUS0_004517"
     CDS             complement(388..1338)
                     /gene="virK"
                     /locus_tag="ACIUS0_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HGB0221943.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSGYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1343..>1685)
                     /gene="capU"
                     /locus_tag="ACIUS0_004518"
     CDS             complement(1343..>1685)
                     /gene="capU"
                     /locus_tag="ACIUS0_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HGB0221944.1"
                     /translation="DNVFPAAPVYRVASLVVLPSENESFGMVLAEASAFSVPVVATQI
                     GGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKT
                     ALKILTLAKQK"
BASE COUNT          511 a          375 c          322 g          477 t
ORIGIN      
        1 ttttccggac cacgaattgc cagttctgcc atcatcacta tggattgcaa tgctgtgaca
       61 aacattttgt cgacaattat ttcccgttct gtatcactct tttctgggaa acaaagcgat
      121 aaattaatcg tcgccctgcg acgagctttt ttgctcaatc tgcccgccca acgccctatt
      181 tttgccaaga caggatctct gaatgaaggg ggaaacattg aaatacctgc aatcattccc
      241 aaaaggaacc atgtagacca gtaaacaggg gaaaggtaat ttttcttaaa ttcaggaata
      301 aactcagatt tatatttttt cattatttag tgcgaatgcc tctaccaaaa ttatcattac
      361 tgttttattc agagaataca tcaaacctta atttaaattc tgatgttctg agtgtacagg
      421 atatgcatcc aaagagcgga tagtagtttc atggatataa tccagcaaag cgtatcgttt
      481 tcggtaccca gagcgttttt tactggctat atcgcttagc ggctttcgaa tcacctgggt
      541 tggtaatctg taccagtcac cacaggtttt tcctgctaca gactcccaga aatcactata
      601 gaccgcaaca aaggttttgc gtttctgata ccagtatctc aattgccgga aaacatgact
      661 gtgctcactg actgccagac actcagagat attgcaagct ttcatcaacg cacaaaatgc
      721 ttcaaaaata atccgttttg ggaaaatccc ataacaggcc cgggttgcgc accgaataac
      781 atcaggtccg gtatttttag gggctccctg taatccgcca ataaaagcaa tgtgtccatt
      841 ttgagtcaga atgactgaaa atgttaaccg ggcaataact gttttattgt aaaacaatga
      901 caacatcaac tctccttccc ggtcaaaacc actgggagaa cactggatat caaaattctc
      961 tccatctttc ccgatgaatt ggactaatga taccgatttt tgtggaaaca aaaatttaga
     1021 atatttttct ggcaaatttt gcacaaagcg ataatgctca agaatatatt gtccacgggt
     1081 ccaggcgcgc ccccctttat gcaaataagg gcgatgaatt cttgctggca acgtggggtg
     1141 tattgccaga atatcctcaa tgcacggcaa ggaacgcagt tcataataat atcgaatact
     1201 gacaaccgga tttatagaac atcgcaaaat gtacatgaac cgaaattttc tgtgttccca
     1261 cttccccggt agtgaatctg aggaaaaaat aatcctttta aggaaaccga taaatgataa
     1321 gttacttata gaaaacatat tgttactttt gcttcgctaa tgtgagtatt tttaacgcag
     1381 ttttattaat atcaaaccgc tcttctatat cctgttttgc caggcgagcc atctgataaa
     1441 aacgcccagg gtcattaaaa aaatcattca gggcgcacat ccatgcgtgc ttattacttg
     1501 ctggtaacaa tgtcccggtc tggttgttct gaataacctc agggattcca ccaatctgag
     1561 tggccactac aggcacagaa aatgccgatg cttctgccag caccatacca aaagattcgt
     1621 tttctgaagg cagaaccacc agactggcaa cccgatatac gggggcggca ggaaaaacat
     1681 tgtca
//
LOCUS       DBBBSB010000323         1668 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.331, whole genome shotgun sequence.
ACCESSION   DBBBSB010000323 DBBBSB010000000
VERSION     DBBBSB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.331"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..454
                     /locus_tag="ACIUS0_004519"
     CDS             <1..454
                     /locus_tag="ACIUS0_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221945.1"
                     /translation="SISWSHYLSPDTNAATVNQTLKCDSKARADQPGACPTTYELYEG
                     DATYRAAIDKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWD
                     YIVTLYNPKTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            574..747
                     /locus_tag="ACIUS0_004520"
     CDS             574..747
                     /locus_tag="ACIUS0_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGB0221946.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            847..1668
                     /locus_tag="ACIUS0_004521"
     CDS             847..1668
                     /locus_tag="ACIUS0_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGB0221947.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          435 a          420 c          424 g          389 t
ORIGIN      
        1 atcaataagt tggagtcatt acctaagtcc tgacacaaac gccgccactg taaatcagac
       61 acttaaatgc gactcaaaag cccgggctga ccagcctggt gcatgtccaa caacgtacga
      121 gttatacgaa ggtgacgcta cctacagagc tgctatcgac aaagcattaa accctgttgg
      181 gttaaacgga atgtttgggg aagacggcta tatggatggt cctgatggtg gtgcgtatcc
      241 tgtaaacatt aacggaacga cctgggtaga aggtggcggt tgtaaagccc acgcctgcgg
      301 ctgggactat atcgtaacac tctataaccc aaaaacgcat aaagtcgttg gttactatta
      361 caacatcgac cctggttacc tgatttggtt tggtgaaact ggcgttcacg aattcgcgta
      421 cctggtcagg gactacgtaa acaaaacaaa ttaaaggcgt aagttatgcg gttcatcttc
      481 gcgttattaa tttattgata tcaaaagtaa atacggctct gccatgatga cagggactta
      541 tattttcagg gaaccaaata caggtctggt tgtatgtcac gtttattgcc cgaagtccct
      601 ttctcacgcg agcaggctgt tgtcatcaca acagcttacc gcaatgtgtt tatcgacgat
      661 gaccagggaa ctcatttccg tcgggttatc cgcaacgcag agactgacgc cggaaggttc
      721 gccagtgacg gtcttctcag acaataaacc tcattaattt cgtccatcag gccgcgtctt
      781 ctccgggaga cgtggctttt tcatttatta caccagtaaa tcttaaccac cgataaggag
      841 caaattatgc gactagccag tcgttttggt tatgctgcaa accagatacg ccgtgaccgt
      901 ccgctgacac acgaagaact gatgcactat gttcccagta ttttcggtga agaccggcat
      961 acttcccgca gtaaacgcta tgcgtacact cccaccatca ccgtactgga aagcctgcag
     1021 cgggaaggct ttcagccatt cttcgcctgc cagacacgtg tgcgtgaccc gggtcgccgg
     1081 gaatacacaa aacacatgct gcgtctgcgg cgggccggac agataaccgg tcagcatgtc
     1141 cctgaaatta ttctgctcaa ctctcatgac ggttcatcca gctaccagat gttacccgga
     1201 tattttcgtg ccatttgtac caatggactg gtctgcggtc agtctctggg agaattgcgt
     1261 gttccacacc ggggaaatgt ggtggagaaa gttattgaag gggcttacga ggtggtgggc
     1321 gtgtttgacc ggatagagga gaagcgtgat gccatgcagt cgctggtcct gccgccaccg
     1381 gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga acatcagcca
     1441 gtcaccaccg ctgacattct gacaccacga cgcagggagg attacggtaa agacctgtgg
     1501 agcgcatatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg ccgcagtgca
     1561 aaaggaaaac gtatccacac ccgtgccatt cacaacatcg acaccgatat taagctcaac
     1621 cgcgcattgt gggtaatggc agaaacgctg ctggagagcc tgcgctga
//
LOCUS       DBBBSB010000324         1657 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.332, whole genome shotgun sequence.
ACCESSION   DBBBSB010000324 DBBBSB010000000
VERSION     DBBBSB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.332"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(5..508)
                     /locus_tag="ACIUS0_004522"
     CDS             complement(5..508)
                     /locus_tag="ACIUS0_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGB0221948.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="ACIUS0_004523"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="ACIUS0_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          471 a          358 c          335 g          493 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgtggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1621 tatcacaact tgaaataaag tcagaaatta aatatta
//
LOCUS       DBBBSB010000325         1655 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.333, whole genome shotgun sequence.
ACCESSION   DBBBSB010000325 DBBBSB010000000
VERSION     DBBBSB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.333"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..190
                     /locus_tag="ACIUS0_004524"
     CDS             <1..190
                     /locus_tag="ACIUS0_004524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221949.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACIUS0_004525"
     CDS             complement(156..1391)
                     /locus_tag="ACIUS0_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGB0221950.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACIUS0_004526"
     tRNA            complement(1538..1614)
                     /locus_tag="ACIUS0_004526"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          450 c          394 g          391 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DBBBSB010000326         1603 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.334, whole genome shotgun sequence.
ACCESSION   DBBBSB010000326 DBBBSB010000000
VERSION     DBBBSB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.334"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(335..481)
                     /locus_tag="ACIUS0_004527"
                     /pseudo
     CDS             complement(335..481)
                     /locus_tag="ACIUS0_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            535..717
                     /locus_tag="ACIUS0_004528"
     CDS             535..717
                     /locus_tag="ACIUS0_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGB0221951.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(818..1147)
                     /locus_tag="ACIUS0_004529"
     CDS             complement(818..1147)
                     /locus_tag="ACIUS0_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGB0221952.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1319..>1603)
                     /locus_tag="ACIUS0_004530"
     CDS             complement(1319..>1603)
                     /locus_tag="ACIUS0_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221953.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          377 g          422 t
ORIGIN      
        1 atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag aataaccagt
       61 tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg gcgacggcgg
      121 aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc tctgtaatgc
      181 ggtatccaac ccgcgaatat cagcatgatt caccgtcgga tttactgcta cgtcgccctc
      241 agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg acacggaaag
      301 tcatatcagc gtcaacggag caccgtattg acgcttattt attggtgaga actacgttcc
      361 atggcaggag ttcgtcaaca cggttggagg gccattcagg cagtatgctc agaatatggc
      421 gcagatacgc ttccggatcg ataccgttca gacggcaggt gcctgctttt ccggcaacaa
      481 gcaggaatct cagcgctgaa gcaggaatta gaggtaaaaa cgccctaccg ggccatgaat
      541 cacccggtca ttggagtagt aaccaaagca gatttagcca gcatggaaca aatatctttg
      601 gtgaaaagtt ggttacggga agctggagcg cacaacgtgt tagtaaccag tgcagttaat
      661 aacaatggag ttacagaact ttttgccttg ctgcatacag aagaaggctg ttgttaattt
      721 cgattggttc tcagatgaga atgatgacgg tgatacacag ataagaatca atgagcaatg
      781 agggttgccg ggcaaccctc attgaataaa acgggaatta cttcgcttcg ccgtttttca
      841 tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag agctcggcat
      901 ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg gcgatgatgc
      961 cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg tccagcaaca
     1021 ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca cgttgcagtt
     1081 tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa ggcttggtag
     1141 tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa ccgctattcg
     1201 ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc ttttcactat
     1261 catacccttt atccttgctg aatcgaagca gcagcaagat gattctgaag ttcaggaatt
     1321 atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt gccgtttcca
     1381 tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca tggtggttat
     1441 cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg gcaaaaaccg
     1501 gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc tgcatcatgt
     1561 gctgggtatc acaaagtttt tgcgcgttca ataacacgaa atg
//
LOCUS       DBBBSB010000327         1581 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.335, whole genome shotgun sequence.
ACCESSION   DBBBSB010000327 DBBBSB010000000
VERSION     DBBBSB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.335"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACIUS0_004531"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACIUS0_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGB0221954.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACIUS0_004532"
     CDS             complement(1269..>1581)
                     /locus_tag="ACIUS0_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HGB0221955.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DBBBSB010000328         1565 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.336, whole genome shotgun sequence.
ACCESSION   DBBBSB010000328 DBBBSB010000000
VERSION     DBBBSB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.336"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..294
                     /locus_tag="ACIUS0_004533"
     CDS             <1..294
                     /locus_tag="ACIUS0_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGB0221956.1"
                     /translation="SAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFE
                     SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            300..1397
                     /locus_tag="ACIUS0_004534"
     CDS             300..1397
                     /locus_tag="ACIUS0_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGB0221957.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          484 a          355 c          387 g          339 t
ORIGIN      
        1 agcgcttatc cgatttttga tcgtattgaa gaaatggcat gggctcgtca ctaccagcag
       61 atcgttcgtg aagaaaaaga aacggaactg gcggacgacc tggaaaaagg tctgcctcag
      121 cacctgtttg aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca
      181 atcagccacg catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg
      241 tacatggctg aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta
      301 tggcgctttt tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt
      361 ttgctggcga cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta
      421 tgcggcaacg gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag
      481 gctctgactg ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa
      541 cccgctcgtt tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa
      601 tgatcatcga ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc
      661 gaaaccgtaa acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac
      721 gctttaccga tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat
      781 atgagtatga ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa
      841 aaatgaaagc agaaacagaa accggttatg aaccctccat ccggatccag atggagaagc
      901 aaatgaacat agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca
      961 cccgtattga tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg
     1021 agtctcttaa cctgggaggt gaacaccccg gcatcgatac ctcacgggat aacagtgaat
     1081 tatttgccaa cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac
     1141 ttgatgaaat tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac
     1201 gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag
     1261 gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg
     1321 ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca ggatacgatg
     1381 agaatattcc ggtgtaaaac caagtgcatt ttgggaatga accgccccgg gaatcctgga
     1441 gactaaactt cctgagaaag aggtaaacag gatgactaaa aatactcgtt tttcccccga
     1501 agtccgtcaa cgggcagtcc gtatggttct ggaaagtcag ggcgaatatg actcacaatg
     1561 ggcga
//
LOCUS       DBBBSB010000329         1561 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.337, whole genome shotgun sequence.
ACCESSION   DBBBSB010000329 DBBBSB010000000
VERSION     DBBBSB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.337"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            400..1149
                     /gene="phoC"
                     /locus_tag="ACIUS0_004535"
     CDS             400..1149
                     /gene="phoC"
                     /locus_tag="ACIUS0_004535"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HGB0221958.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1204..1308)
                     /locus_tag="ACIUS0_004536"
                     /pseudo
     CDS             complement(1204..1308)
                     /locus_tag="ACIUS0_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1319..>1561)
                     /locus_tag="ACIUS0_004537"
     CDS             complement(1319..>1561)
                     /locus_tag="ACIUS0_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221959.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          426 a          332 c          316 g          487 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta tttttcagct
     1561 c
//
LOCUS       DBBBSB010000330         1557 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.338, whole genome shotgun sequence.
ACCESSION   DBBBSB010000330 DBBBSB010000000
VERSION     DBBBSB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.338"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            624..1223
                     /locus_tag="ACIUS0_004538"
     CDS             624..1223
                     /locus_tag="ACIUS0_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HGB0221960.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          408 a          373 c          354 g          422 t
ORIGIN      
        1 tggcttcacc cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct
       61 gctcctgcaa ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt
      121 ataccttatg tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt
      181 atctggtacg ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata
      241 ccttcagcat ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga
      301 taatgtcagg ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt
      361 aaaacctgcg taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata
      421 aaggcctttc cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct
      481 gttacgtatg ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac
      541 acgttcaccc tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt
      601 gttacagaac acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg
      661 ctccccagct cctggagcac tttaaccagc atctggattc attattcggg gtctattcga
      721 aactgttacc gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg
      781 cctgcaggta cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac
      841 tggtcgggta tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg
      901 tgggctatct gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag
      961 atatctggag gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa
     1021 attatccggt aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt
     1081 tacgctatgc catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga
     1141 aatgcagcgg gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc
     1201 cggggatatg tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt
     1261 gctgtcggtt ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc
     1321 gtatgaacca tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga
     1381 tacagcacat cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca
     1441 ttgtgtgaca accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca
     1501 gattttaact gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggctg
//
LOCUS       DBBBSB010000331         1549 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.339, whole genome shotgun sequence.
ACCESSION   DBBBSB010000331 DBBBSB010000000
VERSION     DBBBSB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.339"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..490
                     /locus_tag="ACIUS0_004539"
     CDS             <1..490
                     /locus_tag="ACIUS0_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGB0221961.1"
                     /translation="RFQNGVAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRP
                     VVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHE
                     MYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVA
                     SI"
     gene            493..1038
                     /locus_tag="ACIUS0_004540"
     CDS             493..1038
                     /locus_tag="ACIUS0_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGB0221962.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            1062..>1549
                     /locus_tag="ACIUS0_004541"
     CDS             1062..>1549
                     /locus_tag="ACIUS0_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGB0221963.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLS"
BASE COUNT          429 a          348 c          420 g          352 t
ORIGIN      
        1 ccgctttcag aatggagtgg ctattgcttt taagaatatt caggccggaa ctgccagaaa
       61 attcacgtta tccagcgcca acaactccac gaaaaatgca gcgttttatt tgtggggtaa
      121 tccatccagg cctgttgttg cagagcttgg tgatgattca ggctggcatt ttttcagcca
      181 gagaaacccg gataacagca tagtgttcac tgttaacgga caggtaattc cgttaaatta
      241 cggaaacttc gatgcccgct ataaatatcg aacagagggg gtacaggatg tacggtatgg
      301 ccatgaaatg tattacagcc ccggcagtaa caccgtttcg tggagatttt gcgcaccttc
      361 gggacacggg ctgtcaggga tggcgatatc ggataccggc cgtaactcag cggataacgt
      421 tgacggtgtg tattaccgac cactgcaaaa actgattaat ggcacctggt ataacgtagc
      481 gagtatttaa caatgttgca tttaaaaaat attactgcgg gtaatccgaa aacagcagaa
      541 caatatcaga tgacaaaacg atattcggtc acctggcttt tttcagaaga cggaaaaaac
      601 tggtatgaag agctgaagaa tttcgccagc gacacaataa aaatagctta caccggagat
      661 ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcatcg agccacgcaa tgccagcgtt
      721 atcgaagttc cggatattac cgctaaccga cggattaccg cgccgggtta ctggttttac
      781 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
      841 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggttaatc
      901 agcgacgaag ataaagaaaa gctgaaagcc tgtcgtattt acgcgaaatc gctgcaggcg
      961 atggatttca gcaccatcac tgataaatcc tcatacaacg ccattgaatg gcccgcctct
     1021 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac aatgtctgta gtgatatcag
     1081 gtgcgctgac tgatggcgca ggtatcccca tgtccggata ccatattatt ctgaaatccc
     1141 gggtaaaccc cccggaagtg gtgatgaaca ctgttgctga tgtgatgaca ggaaacgatg
     1201 gtgaatactg tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc
     1261 gcaacgagta caacgttggc gacattgctg tatatgagga ctcaaagccc ggcacgctga
     1321 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgctttg
     1381 aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc agccggaaac gcacagcaga
     1441 cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc agcagagcag gccaaaaatg
     1501 acattgatgc tgcgctgacc ggcactctga aaacggctaa ccatctgtc
//
LOCUS       DBBBSB010000332         1503 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.340, whole genome shotgun sequence.
ACCESSION   DBBBSB010000332 DBBBSB010000000
VERSION     DBBBSB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.340"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..243
                     /locus_tag="ACIUS0_004542"
     CDS             <1..243
                     /locus_tag="ACIUS0_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0221964.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(280..411)
                     /locus_tag="ACIUS0_004543"
     CDS             complement(280..411)
                     /locus_tag="ACIUS0_004543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221965.1"
                     /translation="MARSAKSRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFD"
     gene            493..686
                     /locus_tag="ACIUS0_004544"
                     /pseudo
     CDS             493..686
                     /locus_tag="ACIUS0_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            829..1050
                     /locus_tag="ACIUS0_004545"
                     /pseudo
     CDS             829..1050
                     /locus_tag="ACIUS0_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1071..1133)
                     /locus_tag="ACIUS0_004546"
                     /pseudo
     CDS             complement(1071..1133)
                     /locus_tag="ACIUS0_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          467 a          330 c          334 g          372 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca accgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctagtct ccaggattcc cggggcggtt caatcaaagc gcagaacttc
      301 tgcgtcttct tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt
      361 gcttctttga gaggcgggtt tacgtttacg ggatttagct gaacgggcca tataaccacc
      421 tgaaagacaa tgacattgcc tgtttttata acggtaattg cagaccatga caaaccgcag
      481 ccgtcaggct gcctactcga gtcacaaggc tctttgtgtc cgatatgccg gcagcgcatt
      541 acccatgaaa ctggctggaa cgtacaccat aaggtgaaga aagttatggg ggcggtgaag
      601 aattatctaa ccttgtcttg ttacatccaa attgccatcg acagttacac agttgtgaag
      661 ccggctctct caaaaagggc ttataaaggc ttgagcggtg taccgggaaa ccggtatgca
      721 ccgttcttag gggagggcag tccagcaatg aactgtcctt acccgacaaa catccaaaac
      781 gaacggaacg tgctggaatc cgcatataac ccgttgtaaa gtgaggaaat ggacacctca
      841 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga cactatccac
      901 cagatggctg aatacaaccg cctgctctca cagcggatgg cggcttatgc ttccgaaatc
      961 aaccgactga aggcgctggt tgcgaaactg cagcgtatgc agttcggtaa aagctcagaa
     1021 aaacttcgcg caaaaaccga acggcagatt ccattttcac gggcgatcta cgccacacag
     1081 gctctgggtt gcagtgactg ctccattaag gcgattttga actcataagg gaagtaacaa
     1141 aaaaactgca aaagtacaat aattatatat ggccaaacta acctctatta aaaaacatca
     1201 gtttttcaca cttagttgag cttcatgaaa aaatcatcga ggatttcatt gcagaataca
     1261 cattccacta actggaagca ctccataaga ggaatcagag aaagtatcgt ttttttatag
     1321 taaaattcat tgctgttcaa taaaattgat atagttatta aattatgaaa ataatttcac
     1381 aaaaaagatt aacttgagtt cttaagcatc ggattttaga tattttgaaa tatctggcat
     1441 cacataacat caaaagtata taatactctt atctaattac atgcagtata ttgtatatat
     1501 act
//
LOCUS       DBBBSB010000333         1498 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.341, whole genome shotgun sequence.
ACCESSION   DBBBSB010000333 DBBBSB010000000
VERSION     DBBBSB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.341"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            495..664
                     /locus_tag="ACIUS0_004547"
                     /pseudo
     CDS             495..664
                     /locus_tag="ACIUS0_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            810..>1498
                     /locus_tag="ACIUS0_004548"
     CDS             810..>1498
                     /locus_tag="ACIUS0_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGB0221966.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          366 a          340 c          462 g          330 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagatgccaa cacaaaggca actaataaag atacagataa agatctaaaa acagatctaa
      121 cccatcccaa acccttccct tccggaaggg agtttcggga ttttgtggct ggagtgcttg
      181 aggggagatt atctggcggt actgcagcgg aattttgtaa ttctgcggtg gttgcgttgc
      241 aggctgctgg cctggatgtc tgtcgtgagt atccggtgcc agagcgtggt gacggttgcg
      301 gagggcggat tgatatcgtc gtgactgaca ggaacggtgt ccggtgtggg atcgagcttg
      361 accgaaattc tccgcgacag aaatcactgc tcaaaatcgg tgctgttgaa accgggatat
      421 gtgtcttgcg gcgcagtgat atcgcaaggc acaccgagca gggaattctg gttatcggtg
      481 gggctgttcg ccagaaaaaa tttgacccgt tgtcagttga tctgcccgac tggttgtcag
      541 aaacactctg gcatgagtgg gtccagttca ggcaggcatt gcgaaaaccg attcgaacgg
      601 agctgggcgc taacggggcg atacgggaac tggaaaaatt ccgtcagcag ggttttacac
      661 ctgagcaggt gattcgacac agcatcgcca atgaatacca gggtctgttc gcgccgaaag
      721 gtgttcggcc tgagacgttg ctccgacagg ttaacaccgt ctcgttgccg gacagtgcga
      781 tcccgccagg cttcaggggg taacagacca tgaaaaatat tgcgacagga ggcgttctgg
      841 agcgtatccg cagactgacc ccaccacatg taaccgcccc attcagaacg gttgcggagt
      901 ggcgcgagtg gcaacttgct gaaggccaga aacgttgcga ggagatcaac cgcctgaatc
      961 gtcagttgcg ggtggaaaaa atcctgaacc gctccggcat ccagccgttg caccggaagt
     1021 gttcgtttgc gaattaccgg gcgcagaacg acggtcagcg acatgcactg agtcaggcga
     1081 aatccatagc tgacgaactg atgacaggct gtacgaattt tgtgttcagc ggtaagcccg
     1141 gcaccggaaa aaatcatctt gcagccgcga ttggcaaccg gctgatggcg aaggggcgta
     1201 gcgtgattat cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc tacgacaacg
     1261 gcaaatccgg tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg gtcctggatg
     1321 aaattggcat gcagcgggat acgaaaaacg agcaggtggt actgaaccag attgttgatc
     1381 gccggacggc atcgttacgc ggtgtgggga tgctgacaaa tattaaccat gcagcgatga
     1441 atacacttct cggcgagcgg gtgatggatc gcatggtcat gaacggcggg cgctgggt
//
LOCUS       DBBBSB010000334         1429 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.342, whole genome shotgun sequence.
ACCESSION   DBBBSB010000334 DBBBSB010000000
VERSION     DBBBSB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.342"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..661
                     /gene="rfaY"
                     /locus_tag="ACIUS0_004549"
     CDS             <1..661
                     /gene="rfaY"
                     /locus_tag="ACIUS0_004549"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGB0221967.1"
                     /translation="NDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLK
                     VYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYY
                     IMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLI
                     DLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKS
                     K"
     gene            686..>1429
                     /locus_tag="ACIUS0_004550"
     CDS             686..>1429
                     /locus_tag="ACIUS0_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGB0221968.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEE"
BASE COUNT          515 a          191 c          254 g          469 t
ORIGIN      
        1 taatgatccc ttttatgagc aagttctaaa tgacttttta acttgcaggg ttaaaacatt
       61 aaaagttttt cgttctattg atgatactaa agtcattctt attgacactg ctcgtggacc
      121 tcttgtactt aaagtttatg cgccaaagca taaaatgacc gaacgtttct taaaatcttg
      181 cataaaaaaa gattattatg aaaatttaat ttatcaaacc gatcgggtac gtggtgaagg
      241 catccaatca attaatgact attttttgct agctgaacgt aaaacattaa atttcgctca
      301 ttattatatt atgctcattg agtatattga aggtgtcggg ttaaatgagt atttggaaat
      361 ttctgaagat ctgaaggatc aattatcaga atcgataaaa gagttgcatc agcatggaat
      421 ggtgtctggc gatcctcata aaggaaattt tatagtatcc gaaaaaggtt taagactaat
      481 tgacctttca ggtaaaaaaa caaccgctgt tttaaaagca aaagatcgca ttgatcttga
      541 acggcactat aatataaaaa atgaattgaa agactttggc tatacatatc ttatattcaa
      601 aaagaaaatt aaaaaagtaa tcagggatgt aaaagtcaaa ctaggattga agagcaaata
      661 atgattatga tgaagggtaa ttaaattgga ttttaaacat cttactcaat ttaaagatat
      721 aattgaactg gacaagcgcc ccgttaaact tgatgaacgg gaaacgttta atgtctcatg
      781 gggtattgat gagaactacc aggttggggc tgcgatttca attgcttcaa ttcttgaaaa
      841 gaataaacaa aacaaattta cctttcacat aatcgctgat tacttagaca aagagtatat
      901 tgaattatta tcacaattag caacgaagta tcaaacagta attaaattat atcatattga
      961 ttctgagcca ttgaaggcgc tacctcaatc aaatatctgg ccagtatcta tttattatcg
     1021 tttgctttca tttgattatt tttctgcgcg attggattca ttattatatc ttgatgctga
     1081 tatcgtctgt aagggttcat tgaacgagtt aatagcatta gagtttaaag atgaatatgg
     1141 ggcagtggta attgatgtag atgctatgca aagtaaaagc gctgagcgtt tgtgtaatga
     1201 ggattttaac ggtagctatt ttaactctgg tgtaatgtat attaatttac gggaatggtt
     1261 aaaacaaaga ctaacggaaa aattctttga tctattatca gatgagtcaa ttataaaaaa
     1321 attaaagtac ccggatcaag atattttaaa cttaatgttt ctacatcatg ctaaaatatt
     1381 accgagaaaa tataattgta tttatactat aaagtcagaa tttgaagaa
//
LOCUS       DBBBSB010000335         1404 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.343, whole genome shotgun sequence.
ACCESSION   DBBBSB010000335 DBBBSB010000000
VERSION     DBBBSB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.343"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..167
                     /locus_tag="ACIUS0_004551"
     CDS             <1..167
                     /locus_tag="ACIUS0_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGB0221969.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACIUS0_004552"
     CDS             152..790
                     /locus_tag="ACIUS0_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGB0221970.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="ACIUS0_004553"
     CDS             1018..>1404
                     /locus_tag="ACIUS0_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221971.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          256 c          347 g          394 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg tctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DBBBSB010000336         1356 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.344, whole genome shotgun sequence.
ACCESSION   DBBBSB010000336 DBBBSB010000000
VERSION     DBBBSB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.344"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(41..100)
                     /locus_tag="ACIUS0_004554"
     CDS             complement(41..100)
                     /locus_tag="ACIUS0_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGB0221972.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACIUS0_004555"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACIUS0_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            complement(1281..>1356)
                     /locus_tag="ACIUS0_004556"
     CDS             complement(1281..>1356)
                     /locus_tag="ACIUS0_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGB0221973.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DBBBSB010000337         1356 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.345, whole genome shotgun sequence.
ACCESSION   DBBBSB010000337 DBBBSB010000000
VERSION     DBBBSB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.345"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(3..485)
                     /locus_tag="ACIUS0_004557"
     CDS             complement(3..485)
                     /locus_tag="ACIUS0_004557"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGB0221974.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="ACIUS0_004558"
     CDS             complement(476..778)
                     /locus_tag="ACIUS0_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGB0221975.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="ACIUS0_004559"
     CDS             1050..1322
                     /locus_tag="ACIUS0_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221976.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          399 a          342 c          287 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtaccca gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DBBBSB010000338         1348 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.346, whole genome shotgun sequence.
ACCESSION   DBBBSB010000338 DBBBSB010000000
VERSION     DBBBSB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.346"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACIUS0_004560"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACIUS0_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACIUS0_004561"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACIUS0_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGB0221977.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          370 a          312 c          284 g          382 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcc
//
LOCUS       DBBBSB010000339         1336 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.347, whole genome shotgun sequence.
ACCESSION   DBBBSB010000339 DBBBSB010000000
VERSION     DBBBSB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.347"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACIUS0_004562"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACIUS0_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGB0221978.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACIUS0_004563"
     CDS             complement(590..1255)
                     /locus_tag="ACIUS0_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HGB0221979.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DBBBSB010000340         1286 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.348, whole genome shotgun sequence.
ACCESSION   DBBBSB010000340 DBBBSB010000000
VERSION     DBBBSB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.348"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            231..410
                     /locus_tag="ACIUS0_004564"
                     /pseudo
     CDS             231..410
                     /locus_tag="ACIUS0_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(427..1117)
                     /locus_tag="ACIUS0_004565"
                     /pseudo
     CDS             complement(427..1117)
                     /locus_tag="ACIUS0_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1196..1286
                     /locus_tag="ACIUS0_004566"
                     /pseudo
     CDS             1196..1286
                     /locus_tag="ACIUS0_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          354 a          274 c          313 g          345 t
ORIGIN      
        1 ccattttaca cataacagaa gttagttgta atttatttat ggaggttttc tatggaattc
       61 tttttttatt gtggctgcag tcaataataa cactgaattc aacattccag tgagcaagga
      121 aatgtaagcc gagtaagtat atttgtttta catcatattt tagtcaagct catttattcc
      181 tattatagaa tgacaaatta aaattactgt aaaaaaacga tacacattta atacgaggga
      241 ggccggcaga aaaacggctt gcggtcaggc aggagaaatg tgtgccgcga atgaagtcct
      301 tgtatgactg gataaagctc cagaggaaaa ctttgtcaaa ccatgctgaa atggtaaagg
      361 cgttcaacta tatcctgaat cactggaatg cactgaatga gttttgccgg gtagtacttc
      421 caatcattag aatatgtgct tttcaatgaa ggaaccgatg actttttcgt ggatttccac
      481 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggggc ggagggtcag
      541 gttgttacgt tcatacgttg cgtgaagatt ttgccggtca ggtgtttctc ctccggcact
      601 tctctggcgt agctgcccca gtcatcgctg gtcaccatgc cgatacagaa cggagtcaac
      661 agggctaaca gttcccgaca ggtctcatca ttccgtggcc cgcaagtgta agccaacaca
      721 ccgccagttt tagtgttgta cgcataccag ggccagtgtt gttgcgcctt gctgccaacg
      781 aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca
      841 gatgttatcc gccggggcga gagcttttta atgtccgtat gacggtattg atgccgattt
      901 tcagtgtcct ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct
      961 ccttaacgcc gagttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg
     1021 agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg
     1081 aattacagcg cggacaatga acagtaacgg tagtcatgga aaacctcaaa taccagtaga
     1141 ttaccagaaa cttctatcga ttgaagacat taccttttgc cgcaataggt ggggtaacat
     1201 tggtgaaaac gcattacgcc gggtggcagt tggaaaaaaa ttatcttttt tcggctctga
     1261 tcgtggtgga gaaaatgcgg caataa
//
LOCUS       DBBBSB010000341         1277 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.349, whole genome shotgun sequence.
ACCESSION   DBBBSB010000341 DBBBSB010000000
VERSION     DBBBSB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.349"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACIUS0_004567"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACIUS0_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HGB0221980.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DBBBSB010000342         1211 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.350, whole genome shotgun sequence.
ACCESSION   DBBBSB010000342 DBBBSB010000000
VERSION     DBBBSB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.350"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..730
                     /locus_tag="ACIUS0_004568"
     CDS             <1..730
                     /locus_tag="ACIUS0_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HGB0221981.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACIUS0_004569"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACIUS0_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          319 a          298 c          265 g          329 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggt acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
     1141 tctgaatcgc cacgggttta acagacacct cagagtcatt taagatggct taaagagagg
     1201 tgcccatgag c
//
LOCUS       DBBBSB010000343         1190 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.351, whole genome shotgun sequence.
ACCESSION   DBBBSB010000343 DBBBSB010000000
VERSION     DBBBSB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.351"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>1190)
                     /gene="ospC4"
                     /locus_tag="ACIUS0_004570"
     CDS             complement(<1..>1190)
                     /gene="ospC4"
                     /locus_tag="ACIUS0_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HGB0221982.1"
                     /translation="ILRVTNSSSSGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQG
                     TSFKSLNLSLEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINS
                     NFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISN
                     HQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNF
                     KEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNID
                     PVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKM
                     AHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGAN
                     YKVL"
BASE COUNT          382 a          191 c          192 g          425 t
ORIGIN      
        1 ctagcacttt atagttggca ccatctttac taagtaagta ttcaacatca tacagattac
       61 aatatctatt gtttttcatt ttctccatct ctgcgacatc ttgttttgta aaattaaatt
      121 tagatagtaa ataaagtgct atatcagctt ttttattgcc taaagaataa gcaagggcct
      181 gatgagccat ttttttatca gtcactttat tgttaatctc ttcataatta gatgctttaa
      241 tcgcatcctc caaagatatg tctctgagtt ttattttttt gaagttatct gtacttagca
      301 ttcttggtat atgatactca agctgaaaca caaagtttat aattctatcc agactaacag
      361 gatcaatatt tttgttataa caaaactctc tgaatccttg atctttactc tttcttataa
      421 aatcaataag atgcaaccca attccaagcc tcatatcttt agtgttaaat attggaacat
      481 cagttttccc ctgattacca tgcacatacc tgctaacttc atccacaacc tctttaaagt
      541 tatctaagaa aaatgattgg tgctcatggt aaaaaacagg tggaatagca ttatcaaagt
      601 gatcggttaa tgtcatatat ccatatggag agtcatgatc aatgatatac gcattggcgc
      661 caaaatctac agtatgatgt gttttattta gtgggagttt ttcttgatga ttggaaattt
      721 cgaccccaaa aaaaacaaaa tcgtggttag agatttcttt aatatcctca gagtatgtat
      781 tcttatgtgt ttgtatattt tcagactgaa ttttatcaaa ggatttaata ttaagaatat
      841 tatttgacag attagagtta ctttgatgtc tgaagttaaa attagaatta ataataatag
      901 aaagtaagtg acgttcgtct gaagaaagag gatgaacttt ttgtattatc tcagagatat
      961 agtgcctgga caccttatcg agatgctcaa gtgaccgaaa tgatgaccgt gctccagatg
     1021 gcgcttcaag agaaagattt agtgatttga aactggtccc ttgagaaaac attttgctat
     1081 aactttgcgc tgctatagac ttccttaaaa agtttttcac agtattagca cagtgatcta
     1141 aatgtttttc acttatgcca gaagaagatg agtttgttac gcgtaatata
//
LOCUS       DBBBSB010000344         1190 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.352, whole genome shotgun sequence.
ACCESSION   DBBBSB010000344 DBBBSB010000000
VERSION     DBBBSB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.352"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..316)
                     /gene="mobF"
                     /locus_tag="ACIUS0_004571"
     CDS             complement(<1..316)
                     /gene="mobF"
                     /locus_tag="ACIUS0_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HGB0221983.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEA"
     gene            398..625
                     /gene="vapB"
                     /locus_tag="ACIUS0_004572"
     CDS             398..625
                     /gene="vapB"
                     /locus_tag="ACIUS0_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGB0221984.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            625..1023
                     /locus_tag="ACIUS0_004573"
     CDS             625..1023
                     /locus_tag="ACIUS0_004573"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGB0221985.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          300 a          331 c          293 g          266 t
ORIGIN      
        1 gtgcttcaat aagacgtttg tcccccccga gcatggccat catggagata cttttgggag
       61 cggagaaggt caggtcgtag ccgggacgat gcctgttact gccatcctgc atgcggctta
      121 aatccgcccc gtccggcagc ctgccctcca gaaggcgggt aaagacatcc ttatcgacac
      181 tgccctgcag ccccagttgt tcagccccct ggccggccca gcgttctccc atgctgccca
      241 gcacatagta attatccttg tcggtataat aattaccggc acttccggcc gatctgacct
      301 gcgcgatact catcataaac gtatatccct ttgacatatc ccggtatcaa tcccacaata
      361 gatatacaca agacatatcc acataaggag gcaaataatg gaaaccaccg tatttctcag
      421 caaccgcagc caggcggtca gactgccaaa agcggttgca ttgccggaaa acgtaaaacg
      481 cgttgaagtg attgctgtcg gacgaaccag aatcattacg ccagccggag agacatggga
      541 cgaatggttc gacggacaca gcgtcagcac cgattttatg gataacaggg aacagcccgg
      601 catgcaagaa agggagtcat tctgatgctg aagtttatgc tcgataccaa catctgcatt
      661 tttacgataa agaacaaacc cgccagcgtc agggagcgtt ttaacctgaa ccaggggaaa
      721 atgtgtatca gttcggtcac cctgatggag ctgatatatg gtgcagaaaa aagccagatg
      781 cctgaacgta atctcgctgt gatcgaggga tttgtttccc gcattgatgt tctggattac
      841 gacgctgctg cagccacaca caccggccag ataagagcag aacttgcccg tcagggacgc
      901 cctgtcgggc catttgatca aatgatcgca ggtcatgccc gcagtcgggg gctgattatt
      961 gtgactaata acacccggga atttgaacgt gtgggcggcc tgagaactga agactggagc
     1021 tgacctgttc atcagaaatc atctcccggc tcaacatcct ccccgcgttc ccggtgcaca
     1081 ttgatgttca cctcttcacg acgctgcatc tgctgccagg tgtccggatg attttcctgc
     1141 tgccatgcct cataagcggc catatccacc acttcaccgg attcactgat
//
LOCUS       DBBBSB010000345         1159 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.353, whole genome shotgun sequence.
ACCESSION   DBBBSB010000345 DBBBSB010000000
VERSION     DBBBSB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.353"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACIUS0_004574"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACIUS0_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGB0221986.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DBBBSB010000346         1126 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.354, whole genome shotgun sequence.
ACCESSION   DBBBSB010000346 DBBBSB010000000
VERSION     DBBBSB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.354"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..318
                     /locus_tag="ACIUS0_004575"
     CDS             <1..318
                     /locus_tag="ACIUS0_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HGB0221987.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
     gene            complement(365..706)
                     /gene="symE"
                     /locus_tag="ACIUS0_004576"
     CDS             complement(365..706)
                     /gene="symE"
                     /locus_tag="ACIUS0_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGB0221988.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(927..>1126)
                     /locus_tag="ACIUS0_004577"
     CDS             complement(927..>1126)
                     /locus_tag="ACIUS0_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGB0221989.1"
                     /translation="IKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVCNGLISY
                     IGIYHKTQLHLADALTDAAIN"
BASE COUNT          304 a          218 c          279 g          325 t
ORIGIN      
        1 ttattcgatc atcttcgcga tgaagttatg cgcctggacg cagggatcac gcaggaagta
       61 ttgaagctat atattgcgtt taaagcggaa acgaattttg ttgatgttgt gccgcaaaaa
      121 agccgactgc gattgtcgct taatatgcag tttcatgaac tggtcgatcc gaaaggtatt
      181 gccaaagatg tgacaaatgt tgggcgctgg ggtaatggcg atgtagaaat tggtttcagc
      241 gacctcgcac aacttcctta cattatggga ttaattcgtc aggcatttga aaaacagatg
      301 gagagcgcgt tggtataacg cggcgaataa tacccggtag cactggcgct accgggtcaa
      361 tgtatcaggc gactttctgt ttacctgtaa ttaccccaat aaattcctgt acctgccttt
      421 gtttacgcgc cgacagctta cacaccttgc gtagtgactg catcagttcg ctctcttcgg
      481 cggcggcggg ttgggcggtg aggacaatac agccttccat cactttgaca tctaccgccg
      541 tgccggtggc aaaaccggcg gcttccagcc actgaccttt cagggtgatg gcgggaatac
      601 ggctgtaatc cgggtaacga ctcgcataac tcacggttaa ttgacgatta tttgccgggg
      661 agacttctgc ttctaacggt tgtgcaatag aatgcgtgtc agtcatgatt actattctct
      721 ataaatagtg attgtgatta gcggtgcgag tgtgttggcg cacatccgca ccgtgctaaa
      781 tacctggata catcatcagt aaatacaggg aaagtccagc taaaaattaa tgaaaataag
      841 atgaaaatta gcgtgaaata taaattttgg tgatgtgaat aaagcggcta tttagccgct
      901 ttattggttt tgtgagggcg agggaattag tttatcgctg cgtcagtgag tgcgtctgca
      961 agatgcagtt gggttttatg ataaatccca atataagaaa taagaccatt gcatactgct
     1021 tcaatagaat ttatcttatc aaatattgct tgttgttcat tatagggggg aatcgcaaca
     1081 ataatattgt aaaaactgtc agaattaagt tttggcattt ttattc
//
LOCUS       DBBBSB010000347         1110 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.355, whole genome shotgun sequence.
ACCESSION   DBBBSB010000347 DBBBSB010000000
VERSION     DBBBSB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.355"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1019
                     /locus_tag="ACIUS0_004578"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACIUS0_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="ACIUS0_004579"
     CDS             992..>1110
                     /locus_tag="ACIUS0_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221990.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DBBBSB010000348         1100 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.356, whole genome shotgun sequence.
ACCESSION   DBBBSB010000348 DBBBSB010000000
VERSION     DBBBSB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.356"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            55..>1100
                     /locus_tag="ACIUS0_004580"
     CDS             55..>1100
                     /locus_tag="ACIUS0_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0221991.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DBBBSB010000349         1095 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.357, whole genome shotgun sequence.
ACCESSION   DBBBSB010000349 DBBBSB010000000
VERSION     DBBBSB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.357"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..104)
                     /locus_tag="ACIUS0_004581"
     CDS             complement(<1..104)
                     /locus_tag="ACIUS0_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HGB0221992.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACIUS0_004582"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACIUS0_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGB0221993.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          309 a          249 c          189 g          348 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa ctttt
//
LOCUS       DBBBSB010000350         1095 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.358, whole genome shotgun sequence.
ACCESSION   DBBBSB010000350 DBBBSB010000000
VERSION     DBBBSB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.358"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(24..827)
                     /gene="dkgB"
                     /locus_tag="ACIUS0_004583"
     CDS             complement(24..827)
                     /gene="dkgB"
                     /locus_tag="ACIUS0_004583"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGB0221994.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(990..1066)
                     /locus_tag="ACIUS0_004584"
     tRNA            complement(990..1066)
                     /locus_tag="ACIUS0_004584"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1030..1032),aa:Asp,seq:gtc)
BASE COUNT          285 a          258 c          249 g          303 t
ORIGIN      
        1 cccggaggag ctgtcagaga ggcttaatcc cattcaggag ccagaccttc cgggctaacc
       61 aggcggtcgt tgcaatccag tgcggcgatc gcttttttat cttcggcatc aagctgtaaa
      121 ttttgtgcct taagattact ttccaggttt ttacgtttag tagaagaagg aattactgag
      181 taaccttccc ccatagccca cgccagaatc acttgtgccg gaatcgcatt gtgtttagct
      241 gcaatacgag caataacctc atctttcagt gccttaccat aagccagcgt catataggaa
      301 gtgatatgaa taccgtgctg tttagcccag gcaaccactt tacggttttg cagataagga
      361 gagagttcaa tctggttggt agcgatgttt tcagcgccaa cagcagcaat cactttttcc
      421 atcaatggga tcgtgaagtt ggaaataccg atctcacgcg tcagcccttg ttttttggct
      481 tccagcagcg cctgcataaa ctcttcaaca gagacttcat cgtttggtga cggccagtgg
      541 attagcgtca gatcaacata atcggtacgc aatttttgca ggctctcttt cagactcggg
      601 atcaatttgt ctttgctgag attttcaatc cagattttag tggtgatgta gagttcatga
      661 cgtggcacgc cactttctgc aatcgcctga cctactgcgg cttcgttatc atagatttgt
      721 gcggtatcaa ttgcgcgata accaagttca agcgccgttt tcacagatga aataacaacg
      781 tcgtctttca gacggaaagt acctaaacca aatgcaggga tagccataat atgcctcttt
      841 taatgctatt ttcgttaact gaggtgatta tgggcttcag actgttgcgg gaaaagagcg
      901 taaaacgcag aggatttttg cgattctagc aataatagat atccagacgc aaaaaaacat
      961 cgagcgttaa ctcgaggctt cttaataagt ggcggaacgg acgggactcg aacccgcgac
     1021 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1081 caaccaccgg tttta
//
LOCUS       DBBBSB010000351         1094 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.359, whole genome shotgun sequence.
ACCESSION   DBBBSB010000351 DBBBSB010000000
VERSION     DBBBSB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.359"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            296..>1094
                     /locus_tag="ACIUS0_004585"
     CDS             296..>1094
                     /locus_tag="ACIUS0_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGB0221995.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          355 a          239 c          211 g          289 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggaagcaata cctggagaaa gagtacaaag caaactgtat taaacttaat
      241 aagttatatt gtatttcact agagttctta tcgttgataa gaaattctgg ttataatgat
      301 taaatcaacg aatatacagg taatcggttc tggtattatg catcaaataa acaacataca
      361 ctcgttaact ctattttctt tgcctgtgag tttgagtcca tcttgtaatg aatattattt
      421 aaaagtctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca atatcgcctt
      481 caataggcta aaaatatgtt tgcaaaatca aggggcggaa ttaaatctat ctgagttaga
      541 tttaaaaaca ttacctgatt taccgcctca gataacaaca ctggaaataa gaaaaaacct
      601 attaacatat ctccctgatt tcccaccaat gcttaaggta atacatgctc aatttaatca
      661 actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgtgg gtgataacaa
      721 gataaaagaa ttaccatatc ttcctgaaac tctaactcat ttacggatcc ataataaccg
      781 attgcatatt ctgccactat tgccaccgga gctaaaatta ctgatagttt ctggaaacag
      841 attagacagc atccccccct ttccagataa gcttgaaggg ctggctctgg ctaataattt
      901 tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa tgaataataa
      961 tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg taaatgttgc
     1021 aggtaatcca ttgtctggcc ataccatgcg tacactacaa caaataacca ccggaccaga
     1081 ttattctggt cctc
//
LOCUS       DBBBSB010000352         1085 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.360, whole genome shotgun sequence.
ACCESSION   DBBBSB010000352 DBBBSB010000000
VERSION     DBBBSB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.360"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..227)
                     /locus_tag="ACIUS0_004586"
                     /pseudo
     CDS             complement(1..227)
                     /locus_tag="ACIUS0_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            211..469
                     /locus_tag="ACIUS0_004587"
                     /pseudo
     CDS             211..469
                     /locus_tag="ACIUS0_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(578..>1085)
                     /locus_tag="ACIUS0_004588"
     CDS             complement(578..>1085)
                     /locus_tag="ACIUS0_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0221996.1"
                     /translation="FNDVVRKAVLSGKINELNACHKVAIFLAEKDNEITKKDKAKIID
                     TLTENYSIEFQQLMNISERTLNSSLYITPGESGFVSFVNREGKICHTAYVKSSDNSMT
                     YYHANGSSIDKYITDMCGLICMRHIESTGIIFYMLDEKVLSAIAEFMNEKGWRAAFCS
                     AKNLYKCV"
BASE COUNT          307 a          253 c          191 g          334 t
ORIGIN      
        1 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
       61 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
      121 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
      181 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
      241 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
      301 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
      361 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
      421 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
      481 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
      541 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
      601 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
      661 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
      721 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
      781 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
      841 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
      901 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
      961 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     1021 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     1081 tgaac
//
LOCUS       DBBBSB010000353         1070 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.361, whole genome shotgun sequence.
ACCESSION   DBBBSB010000353 DBBBSB010000000
VERSION     DBBBSB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.361"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACIUS0_004589"
     CDS             complement(344..1039)
                     /gene="aqpZ"
                     /locus_tag="ACIUS0_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGB0221997.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          301 a          322 c          251 g          196 t
ORIGIN      
        1 tttaatccta tctgagtgtt tagcattgtt gcgctccttt catgtgcaat gccatataaa
       61 gccactggcg gagggagcgc gcagggggca gccagtcgcc gccgccccct gcacccccgg
      121 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg
      181 gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat gtgggctgcc
      241 ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc ggtttgtaat
      301 gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca cgcttttcca
      361 gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      421 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      481 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      541 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg tggatcacta
      601 acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      661 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      721 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      781 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      841 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc atcgtcagaa
      901 cggtcaaacc aaatgccagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      961 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
     1021 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
//
LOCUS       DBBBSB010000354         1068 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.362, whole genome shotgun sequence.
ACCESSION   DBBBSB010000354 DBBBSB010000000
VERSION     DBBBSB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.362"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..560
                     /locus_tag="ACIUS0_004590"
     CDS             <1..560
                     /locus_tag="ACIUS0_004590"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HGB0221998.1"
                     /translation="TKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDN
                     YQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKS
                     GLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMV
                     NEVETALFNARALALKIYSMSGWNL"
     gene            787..>1068
                     /locus_tag="ACIUS0_004591"
     CDS             787..>1068
                     /locus_tag="ACIUS0_004591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HGB0221999.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          314 a          202 c          257 g          295 t
ORIGIN      
        1 acaccaaagg cgatgccatg attggcgtca agcagccttt accaaaagca gttttaagaa
       61 ctcagcatga caaaaataaa gaagcaataa gtatcctgga ttttggtgtt attgatgatg
      121 gtgtgacaga taattaccag gcaatacaaa atgctataga tgccgttgct tcactaccct
      181 ccggcgggga gctgtttatc cctgcgagca accaagcggt ggggtatatt gttggatcca
      241 ctttgcttat tcctggcggt gttaacatca gaggagttgg taaggcatcg caactccgag
      301 caaaaagcgg acttacagga tctgtgttaa ggctgtctta tgattcagac actatcggcc
      361 gttatctgag aaatatacga gtaactggta ataacacctg caatggtatt gacacaaaca
      421 ttacagcaga agactctgtc atcagacagg tttatggctg ggtatttgat aatgtaatgg
      481 tgaatgaagt tgaaaccgca ttatttaatg caagggctct ggcactcaaa atttatagca
      541 tgtcaggctg gaacctgtag agtcggtctt cactttttag gccagtgcgt aagtgttagt
      601 gtcagctcct gccatttcag cagaggaaat tattctgctg atgaaagctt tggcatcagg
      661 attcagcctc aaacgtatgc gtggtcgtca gaggcagtta ggtcagaagc aataatttta
      721 gacagtgaga ccatgtgcat tggttttaaa aatgccgtct atgttcatga ttgccttgat
      781 ttgcatatgg aacaactgga tttagattat tgcggctcaa caggcgtggt tatagagaat
      841 gtaaacggag gattttcttt ctcaaactca tggatagcag cagatgccga tggcactgaa
      901 caatttacgg ggatatattt tagaacgccg gcctcaacgc agtcacataa aattgtcagt
      961 ggtgttcata tcaacactgc aaacaaaaac acggctgcaa acaatcaaag tatagcgata
     1021 gaacagtcgg cgatcttcgt ctttgtaagt ggttgtacgt taactggt
//
LOCUS       DBBBSB010000355         1068 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.363, whole genome shotgun sequence.
ACCESSION   DBBBSB010000355 DBBBSB010000000
VERSION     DBBBSB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.363"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(226..651)
                     /locus_tag="ACIUS0_004592"
     CDS             complement(226..651)
                     /locus_tag="ACIUS0_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGB0222000.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(648..1031)
                     /locus_tag="ACIUS0_004593"
     CDS             complement(648..1031)
                     /locus_tag="ACIUS0_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGB0222001.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          307 c          265 g          243 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcgat aaaaacatcg ccatatcctc
      421 aatgtaaggg tgggacttct ccggattcag caccacacag tccgccttct gttgctcact
      481 cagggcatgt aaatcatgct caaaccacgc cccctgagca tctgtctgca gaatcaaccg
      541 gccatggcag gaaaggcaga aacactgccg gatatttctg ctgagcgcag agtcggcact
      601 gataatgtgc tcacccggcg tgatccccag ccccgttttt ataccgttca ttctgctccc
      661 ccctcctcac tgaagcgccc ggtctggcag tgtgtgcagt actgctcccc ataataatcg
      721 tggtgacatt gcctgcagtg ccagctggct ttacgtacta cgggtaaggt atccgggacg
      781 aattgctgca gacgtttaat caaccgtatt tctctgcgtt ccggcctgac ataagggcac
      841 tcatgaccgt gctccgtcag cccttcgtca gcgtgttcaa accagggccg ttcagtgtcg
      901 tattgcgggt ggtatctgag tgcactgccg caaaggtggc aggtgtagct atcgtaaggt
      961 gcagtctgtg cggtacgggc accggtcaga cgtccgttgc catcaagtgc gagaaaagat
     1021 tttgcgtaca tagtatatgt tcctcagccg ccagacgaca cgcaggca
//
LOCUS       DBBBSB010000356         1066 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.364, whole genome shotgun sequence.
ACCESSION   DBBBSB010000356 DBBBSB010000000
VERSION     DBBBSB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.364"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(570..749)
                     /locus_tag="ACIUS0_004594"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACIUS0_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACIUS0_004595"
     CDS             843..>1066
                     /locus_tag="ACIUS0_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0222002.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DBBBSB010000357          984 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.365, whole genome shotgun sequence.
ACCESSION   DBBBSB010000357 DBBBSB010000000
VERSION     DBBBSB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.365"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..132
                     /locus_tag="ACIUS0_004596"
     CDS             <1..132
                     /locus_tag="ACIUS0_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HGB0222003.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACIUS0_004597"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACIUS0_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACIUS0_004598"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACIUS0_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          313 a          178 c          262 g          231 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat
      961 ttaaggagaa tataaaatga aagt
//
LOCUS       DBBBSB010000358          963 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.366, whole genome shotgun sequence.
ACCESSION   DBBBSB010000358 DBBBSB010000000
VERSION     DBBBSB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.366"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(282..357)
                     /locus_tag="ACIUS0_004599"
     tRNA            complement(282..357)
                     /locus_tag="ACIUS0_004599"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
BASE COUNT          298 a          213 c          149 g          303 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 ccc
//
LOCUS       DBBBSB010000359          935 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.368, whole genome shotgun sequence.
ACCESSION   DBBBSB010000359 DBBBSB010000000
VERSION     DBBBSB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.368"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(57..245)
                     /locus_tag="ACIUS0_004600"
     CDS             complement(57..245)
                     /locus_tag="ACIUS0_004600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222004.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(542..780)
                     /locus_tag="ACIUS0_004601"
                     /pseudo
     CDS             complement(542..780)
                     /locus_tag="ACIUS0_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          246 a          181 c          217 g          291 t
ORIGIN      
        1 taccgcgctg atggttctta tccgttttat tgaatcggag aacaaactgt gagctatcag
       61 cctggtggtc tggttttgcc tgatttaagc cgccgtgggt atttgtccaa gaatgcttca
      121 agctccaatc gttttggcct ggtgaatgga gatgggttaa gcaaagactc tcgttgctgg
      181 tgtacttttt cgcgctggcg ctcaagcatg gataatgcga ttgcctgtag ttcttcgtta
      241 gtcattgtct gcacctggaa agcaatttac cggagtacct cacactacgg caaaaatgtt
      301 ttattgttgc aacttgcgta attattcagg tcagtttgga acgcactgta taaacataaa
      361 taacctgata ctgttgtgtg ttttcaaaga aaacacaggt atttttcaca taaaccttcc
      421 ccttttcata ttcgaccact taatcaaaaa acggttttat acttgttgcc cccactgggg
      481 ttaaatgtga gacatgtcgc actttgtgcc atgtgtcctc gctgatttta ggcgcggttc
      541 attcattctt tctgttggca taaagtatgg tatcggttgg gcgaatgaaa gggactcggg
      601 tcatggtcag acaatacctg aggcgttaat tcgttgaaag agattgtgaa tgggggaata
      661 actgagtctt aactgctgtg cgatagcccg gacagatagt tgctcgtcga atctgagacg
      721 taatgattct ggcaaataag tgcgaaagcc ggtgatatct agtgttgttt gagagttcat
      781 cgttcgcgtc cattaataca gatggacgcg attgttgcca gacaggataa ttttcacaag
      841 acatcgcaga tagagtactt acgataattt tttattttat acaacagatg cccacgaaaa
      901 gaattatttt caatagacac ctgcattaca caaag
//
LOCUS       DBBBSB010000360          931 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.370, whole genome shotgun sequence.
ACCESSION   DBBBSB010000360 DBBBSB010000000
VERSION     DBBBSB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.370"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..269
                     /locus_tag="ACIUS0_004602"
     CDS             <1..269
                     /locus_tag="ACIUS0_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGB0222005.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="ACIUS0_004603"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="ACIUS0_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGB0222006.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>931
                     /locus_tag="ACIUS0_004604"
     CDS             613..>931
                     /locus_tag="ACIUS0_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGB0222007.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          252 a          238 c          259 g          182 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcgcca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac c
//
LOCUS       DBBBSB010000361          922 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.371, whole genome shotgun sequence.
ACCESSION   DBBBSB010000361 DBBBSB010000000
VERSION     DBBBSB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.371"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..199
                     /locus_tag="ACIUS0_004605"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACIUS0_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..>922
                     /locus_tag="ACIUS0_004606"
     CDS             299..>922
                     /locus_tag="ACIUS0_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HGB0222008.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          292 a          217 c          205 g          208 t
ORIGIN      
        1 ttggctacgt aattaaaaca gaaaataaag aaaccccatt cactgtagtt gatatcgacg
       61 gtctatcagg aaactttaaa actcttgttg aaggtgccac aaaaatgtgc ctagtttaca
      121 tcggaaataa tctgcccgca gaaaaaaagc cgaatttctg gcaaatctga ttgcaatgaa
      181 attaaaaggt gaaatctgaa aaaaagaaag cctgcacagt gtgcaggcct gagtgaagaa
      241 cctgggacat ttattcatca ctcgcagtaa ttttaatctg agttgaggtt aaaaaacaat
      301 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      361 cgtcctgtgg gcaagcgacg attttgaatc gaccagcacc actctggact atctgctcgt
      421 taagtcaggt aaaaaactga gcaactattt taaagctgtt gccacaaatt ttcctgtcgt
      481 taatgacctt ccccctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
      541 caaagactcc atgacatggg aactaaaacc gggagcagcg ccagacgacg ttcaccacca
      601 ggataatgct caagaaacca aagaactggc gggaggccag gaagaaaacg cgcaggcaga
      661 cgcccacgag gattgccagg attgcgaagt ctctgtagcc actttgcggt tcacacagcg
      721 tcttctgcac atttttacgt atgctgccgg ggatctgaaa tacctgcatc atgccacccg
      781 tgaacaacgc aaacacatta ctgctcttga gatggatcag gaaaacagct atgtccagaa
      841 tctgctgttg gccatacgcg gcatggcaga accgacaact ctggataatg ccgccctgct
      901 ccgcctgact gatgcaatta ag
//
LOCUS       DBBBSB010000362          887 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.372, whole genome shotgun sequence.
ACCESSION   DBBBSB010000362 DBBBSB010000000
VERSION     DBBBSB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.372"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            270..743
                     /locus_tag="ACIUS0_004607"
     CDS             270..743
                     /locus_tag="ACIUS0_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0222009.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          270 a          186 c          197 g          234 t
ORIGIN      
        1 aatcgctgga ttcggtcatt ctgtctccct tgtatatggg ttaaccagat aaccacgggg
       61 agcaggtacc ggcatccacg ttacagacgg tcccggcaaa agtgcctgac ctgaccccta
      121 ttagcggtta ccagcgcccc accagacccg gtgacatcac cccccggatc atggacgact
      181 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
      241 agtaacgggg gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc
      301 agtgcactga taataaaaag acggagacag atgccattta cgataaagtt cggagttctt
      361 atttgctttc atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat
      421 catttgtttc tgtgtctgaa aaaatcgcca gaattgtgat ggcaaattac tcccgtaact
      481 ggagtaattc cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa
      541 tgtacaaaga ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta
      601 tcaggaaatt acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca
      661 gttcaccatc gtatttttct aaagtcttct ttaaatattt aaagacatac cctcaaaaca
      721 taaggaagaa aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt
      781 acgatactga gatcaaacag acataatcta tgcggagatt tacagcaggg catggttcac
      841 aaatctgaca gcgatgaatt atcggcactg cgggcagaaa atgctcg
//
LOCUS       DBBBSB010000363          873 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.373, whole genome shotgun sequence.
ACCESSION   DBBBSB010000363 DBBBSB010000000
VERSION     DBBBSB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.373"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>873
                     /locus_tag="ACIUS0_004608"
     CDS             <1..>873
                     /locus_tag="ACIUS0_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGB0222010.1"
                     /translation="NLSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVF
                     SKDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRL
                     MGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDA
                     MKKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGS
                     ENGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVT
                     LAYRPLNDTQRKIV"
BASE COUNT          265 a          153 c          209 g          246 t
ORIGIN      
        1 taatttaagt tcagcagcag ttaaaacagc aggtgtactt atcagctttc aggctgtact
       61 atccgcttat cagcgagtaa tggaagttgg ccttcaacgt gcaagtgctg aacgttcaat
      121 tgatttcgta ttttctaaag atgcaaatca agtaaaagat ttcacaaagt cattagctca
      181 aacaacgggt ttagatatcg ctgaactgca atcacagttt gctggtttcg gtgcttcagc
      241 tagagagtct ctaggcattc aaggttctga agaacttttt agaaatatga ttggctattc
      301 tcgtctgatg gggcgaagtg aagaagagat taagcgagca ctgacagcat tatcacagat
      361 ggctggtaag ggtcagatta tggctgaaga gcttaagggg caactagctg aagctgttcc
      421 tggtatggtt caggtattcg ctaaagcaac cggaaagact gaacaggaat tatttgatgc
      481 tatgaagaag ggggctttaa aatctgccga tactcttcag aaggtcactc aggaactgaa
      541 taaacaaatc accgctaaag gtggctggaa agctatttca gaaagtacac aggcacaact
      601 agggaacttg aaaaactctt ggaataccac gcttgatagt atcttcagag gttctgaaaa
      661 tggtctgcaa gattttacaa gaagcttaac caatctttta aacgctcttg gtggaagtgg
      721 taagagtctt ggtgaatctc ttggtagtct catgacttct atgtcacatg gtgtcgattc
      781 attgaccgat attagttaca gggtaagggc gttctttgat gaggtgacat tagcttaccg
      841 tccactaaat gacactcaga ggaaaattgt tga
//
LOCUS       DBBBSB010000364          868 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.374, whole genome shotgun sequence.
ACCESSION   DBBBSB010000364 DBBBSB010000000
VERSION     DBBBSB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.374"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..534
                     /locus_tag="ACIUS0_004609"
                     /pseudo
     CDS             1..534
                     /locus_tag="ACIUS0_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          246 a          204 c          189 g          229 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      601 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      661 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      721 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      781 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      841 aagttagata atatattcta aataacca
//
LOCUS       DBBBSB010000365          851 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.375, whole genome shotgun sequence.
ACCESSION   DBBBSB010000365 DBBBSB010000000
VERSION     DBBBSB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.375"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            59..556
                     /locus_tag="ACIUS0_004610"
                     /pseudo
     CDS             59..556
                     /locus_tag="ACIUS0_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="ACIUS0_004611"
                     /pseudo
     CDS             597..788
                     /locus_tag="ACIUS0_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          250 g          172 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag g
//
LOCUS       DBBBSB010000366          847 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.376, whole genome shotgun sequence.
ACCESSION   DBBBSB010000366 DBBBSB010000000
VERSION     DBBBSB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.376"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..211
                     /locus_tag="ACIUS0_004612"
     CDS             <1..211
                     /locus_tag="ACIUS0_004612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222011.1"
                     /translation="AAGNFRIPFVINTYKPEDQFFVSGIHKERNIQVCATLNGIVKII
                     KAQNLSFFVTGSFKPSGLFPPAPQK"
     gene            242..523
                     /locus_tag="ACIUS0_004613"
                     /pseudo
     CDS             242..523
                     /locus_tag="ACIUS0_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(537..>847)
                     /locus_tag="ACIUS0_004614"
     CDS             complement(537..>847)
                     /locus_tag="ACIUS0_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGB0222012.1"
                     /translation="QELWGVLLAYNLVRYQMIKMAEHLKGYCPNQLSFSESCGMVMRM
                     LMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA
                     "
BASE COUNT          201 a          244 c          178 g          224 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcgta accggctcat ttaaaccgtc
      181 tggtctgttt cctccggctc cacaaaaata atgtccatca ttttgaagtg gacactatcg
      241 tatggaatac cggacctgga tcactgaagc tttacgcctt catttcgaag aacatttacc
      301 acgggttgta gccggacgtc gcctgggcgt accaaaatca actgtctgtg gtatgttcgt
      361 gcgcttccgc aacgctggcc tttcatggcc tctgcccgca ggtatgtcgg agcaggaact
      421 tgatgccctt ctttacgaaa gtgcctccac agtacctgtc gtacttactg aaagcaccgt
      481 tatgcccaaa ctcccggtgg taaaaaaacg tccccggcgg ccttaatgcc agtcagttaa
      541 gcaactgact ggctcttttt cggggctgtg gggtatttcc agggcctctc ctttaccact
      601 ctcgggaagg cccttcccct tctcgtcggt aatttcacaa gttgtcccat acttgcaaga
      661 tcgcgcatca gctccggtat acgtcccggt gaagcgccct gcaatgtcat cagcattctc
      721 atcaccattc cgcatgattc tgagaaactc agttgattcg ggcagtaacc tttcagatgt
      781 tccgccattt taatcatctg atatctcacc agattataag ccagtaagac accccacagc
      841 tcttgct
//
LOCUS       DBBBSB010000367          832 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.377, whole genome shotgun sequence.
ACCESSION   DBBBSB010000367 DBBBSB010000000
VERSION     DBBBSB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.377"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..327
                     /locus_tag="ACIUS0_004615"
     CDS             <1..327
                     /locus_tag="ACIUS0_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGB0222013.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACIUS0_004616"
     CDS             complement(370..570)
                     /gene="glgS"
                     /locus_tag="ACIUS0_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGB0222014.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          155 g          241 t
ORIGIN      
        1 gttagtgaga cgtttagtgt agtcatgaca aaagatcctg gtgcggcctg tactgagcaa
       61 ttcaatattt tagggagctt tttcactacg gatattttga gtgattatag ccatctggat
      121 atgggtaatg gactgctatt gaagatattt cataaagatg gaacagcaac ggaatttaac
      181 cgcttctcac aatttgcttc tttttcatcg tctagtgcac cttcggtcac cgcaccattc
      241 agggcagaac ttagtgcgaa cccggcagag actgttgttg agggaccgtt tagtaaagac
      301 gtaatcctca aaatcaccta taactagcat ctaatacaaa cactaaaacg ggccatcagg
      361 cccgttatat cagtgctcta actccagctc tcttgcctgc atcatctgtt ggcaatacca
      421 ggcataacgt gcgcaaaacc aagttctatg ttcttcatcc atgttaccag taacagccag
      481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcca ggaaatcgaa
      541 attattcaaa gaattaagac tatgctccat aagcacatcc tccggttatc gcataccttt
      601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
      661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaccatatt
      721 aaaatacttt ctcttccaga acttctctaa catgtgttat tattaacaaa gacaaatgta
      781 agcagggaaa ctattccaag gtaaatattg cgtcactaaa tggacattgg ag
//
LOCUS       DBBBSB010000368          807 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.378, whole genome shotgun sequence.
ACCESSION   DBBBSB010000368 DBBBSB010000000
VERSION     DBBBSB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.378"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="ACIUS0_004617"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="ACIUS0_004617"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGB0222015.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          203 a          210 c          177 g          217 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg aacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgtt
//
LOCUS       DBBBSB010000369          803 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.379, whole genome shotgun sequence.
ACCESSION   DBBBSB010000369 DBBBSB010000000
VERSION     DBBBSB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.379"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            46..803
                     /locus_tag="ACIUS0_004618"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACIUS0_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DBBBSB010000370          782 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.380, whole genome shotgun sequence.
ACCESSION   DBBBSB010000370 DBBBSB010000000
VERSION     DBBBSB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.380"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACIUS0_004619"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACIUS0_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HGB0222016.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          246 a          117 c          173 g          246 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 cc
//
LOCUS       DBBBSB010000371          782 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.381, whole genome shotgun sequence.
ACCESSION   DBBBSB010000371 DBBBSB010000000
VERSION     DBBBSB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.381"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..666)
                     /locus_tag="ACIUS0_004620"
     CDS             complement(<1..666)
                     /locus_tag="ACIUS0_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGB0222017.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACIUS0_004621"
     CDS             complement(656..>782)
                     /locus_tag="ACIUS0_004621"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HGB0222018.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DBBBSB010000372          760 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.382, whole genome shotgun sequence.
ACCESSION   DBBBSB010000372 DBBBSB010000000
VERSION     DBBBSB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.382"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>760
                     /locus_tag="ACIUS0_004622"
     CDS             <1..>760
                     /locus_tag="ACIUS0_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGB0222019.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          210 t
ORIGIN      
        1 tgagcgcatt ctgaccgacc gtgaaaatgg tgaaaaaggc gataattctc aagatacctc
       61 ttatgcccgt gtaggcgtga aaggtgaaac tcagatcaac cctgaaatga ctggttacgg
      121 tcagtttgaa ctagacctgg aagcatcaaa cagacacaac cctgaccaaa ctcgtttggc
      181 atatgctggt ttgagttaca aagattttgg ttcattcgac tacagtcgta atgtcggtgt
      241 cgcgtatgat gcagaagcat ttactgatat gtttgttgag tggggcggtg actcatgggc
      301 tggtactgac ctgttcatga ccaaccgtac caacggtgtt gcaacctatc gtaacacaga
      361 tttctttggc atggttgaag gtctaaattt tgctctgcaa tatcagggta aaaatgaagg
      421 tacaggcaac tacaaagcaa atggcgatgg ccatggttta tctgcaactt acactattga
      481 cggtttctca tttgctggtg cttatgcaaa ctctgaccgt actgattggc agtctggtga
      541 tggtaaaggc gaaagagccg aagtttgggc gttgtcaaca aaatatgacg ctaacaatgt
      601 ttatgctgct gtgatgtacg gtgaatctca caacatgaat tccgacgacg gtgatgttgt
      661 gaacaaaaca caaaacttcg aagctgttct gcaatatcag ttcgactttg gtctgcgtcc
      721 atccatcggt tactcctatt ctaaagcact ggacgtagca
//
LOCUS       DBBBSB010000373          756 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.383, whole genome shotgun sequence.
ACCESSION   DBBBSB010000373 DBBBSB010000000
VERSION     DBBBSB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.383"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..158)
                     /locus_tag="ACIUS0_004623"
     CDS             complement(<1..158)
                     /locus_tag="ACIUS0_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGB0222020.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACIUS0_004624"
     CDS             complement(308..469)
                     /locus_tag="ACIUS0_004624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222021.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACIUS0_004625"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACIUS0_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          201 a          218 c          147 g          190 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcag
//
LOCUS       DBBBSB010000374          755 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.384, whole genome shotgun sequence.
ACCESSION   DBBBSB010000374 DBBBSB010000000
VERSION     DBBBSB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.384"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            4..>755
                     /gene="ipaH3"
                     /locus_tag="ACIUS0_004626"
     CDS             4..>755
                     /gene="ipaH3"
                     /locus_tag="ACIUS0_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HGB0222022.1"
                     /translation="MPIMLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          224 a          197 c          128 g          206 t
ORIGIN      
        1 tggatgccca tcatgttacc gataaataat aacttttcat tgccccaaaa ttctttttat
       61 aacactattt ccggtacata tgctgattac ttttcagcat gggataaatg ggaaaaacaa
      121 gcgctccccg gtgaaaatcg gaatgaagcg gtctccctac ttaaagaatg tctcatcaat
      181 cagttcagtg agcttcaact gaatcgttta aatctgtcct cgctacctga caacttacca
      241 cctcaaatca ctgttctgga aattactcag aatgccctaa tatcattacc agaattgcca
      301 gcatcgctgg aataccttga cgcctgtgac aatcgcctgt caacacttcc tgaattaccc
      361 gcatctctga aacatcttga tgtagataac aaccaactaa ccatgcttcc tgaattgcct
      421 gcattgctgg aatatattaa tgcagataac aatcagctaa ccatgcttcc tgaattacct
      481 acatcgctgg aagtgctctc agtaagaaat aaccagctga catttctccc tgagttacct
      541 gaatcactgg aagcgctcga tgtaagtact aatcttctgg aaagcctacc agccgtacct
      601 gtaagaaatc atcactcaga ggaaaccgag atatttttcc ggtgccgcga gaatcgcatc
      661 acacacattc cggaaaatat acttagcctt gatccgacct gcactatcat cctcgaagac
      721 aatcctctgt cctcacggat cagggagtct ctgtc
//
LOCUS       DBBBSB010000375          721 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.385, whole genome shotgun sequence.
ACCESSION   DBBBSB010000375 DBBBSB010000000
VERSION     DBBBSB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.385"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..721)
                     /locus_tag="ACIUS0_004627"
                     /pseudo
     CDS             complement(1..721)
                     /locus_tag="ACIUS0_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          253 a          128 c          100 g          240 t
ORIGIN      
        1 ataaggacaa tggctaaatg ttttcttcat attctcaata aagtttttat taacaatatt
       61 gagaattttt aattgcatca ccagttttac tgccagaacg gatttcaata tcattgcatt
      121 atctggcaat gtatcaatca tttttataat gttattgata taaccatcga gatcttcatt
      181 cccaaatata tctgaataat caatagttaa ttccaattgt tctgtaatgc aatcttaaaa
      241 attccctgtc gacattcgca catgatcaaa cataacagct gtcaaattcg atttaataaa
      301 tgatatttct ttggcagaca gaaactctgc tctggtgaag tgcgaagcgt aaagataaca
      361 attatcgaag tttgaatttc taatctgaca ttcgcgaaaa ttactatgac caagttgact
      421 ctgcgagaag tcaactgtat caagaatatt ttggctaaaa tcaacacatt cgccactcgt
      481 tttgagaaaa atcacattgt gtaaacgtcg acatttaaat atgacattat caactgttgc
      541 cttagtaaaa ttgcaattct caatttcttt acattcgaaa gttacatgtt gtagatttga
      601 tgataaaaaa gacaccttag aaataacgtc acctttaaaa tgacaatttg ttaaatcaaa
      661 atcagacagg tcgatatacc cctgcgcatc tttatccatc agaaaattac aagcgcattc
      721 t
//
LOCUS       DBBBSB010000376          714 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.386, whole genome shotgun sequence.
ACCESSION   DBBBSB010000376 DBBBSB010000000
VERSION     DBBBSB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.386"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..407
                     /locus_tag="ACIUS0_004628"
                     /pseudo
     CDS             1..407
                     /locus_tag="ACIUS0_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(433..>714)
                     /locus_tag="ACIUS0_004629"
     CDS             complement(433..>714)
                     /locus_tag="ACIUS0_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HGB0222023.1"
                     /translation="LRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQA
                     NSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          163 a          177 c          180 g          194 t
ORIGIN      
        1 gtggcggaat gaccattctc agggcattca ttcgttctct ctcggttcct caggagcagg
       61 agcctgccgt tcggttcgaa actgaacccg gacgacagat gcaggttgac tggggcacta
      121 tgcgtaatgg tcgctcaccg cttcacgtgt tcgttgctgt tcccggatac agccgaatgt
      181 tgtacatcga attcactgac aatatgcgtt atgacacgct ggagacctgc catcgtaatg
      241 cgttccgctt ctttggtggt gtgtcgcgcg aagtgttgta tgacaatatg aaaactgtgg
      301 ttctgcaacg tgacgcatat cagaccggtc agcaccggtt ccatccttcg ctgtggcagt
      361 tcggcaagga gatgggcttc tctccccgac tgtgtcgccc cttcaggctg agggatcccc
      421 acaaaattac tgctaataaa ccagcaccat atttcggtag gttctggaca gatggtttag
      481 aactgtgctc agtactgttc gtcttaaaat aagtggcgag tgtcgaagaa cattcttcgc
      541 taacgtcaga tatgagataa cccgccggga tttaatgctg ttagcctgat atttcaggtg
      601 taacccttta ttttcagcgt gataaccgag taaccacatt acgatagtgc tcaacgttgc
      661 cagcagactc agaaccagaa ttcttcctgt tgaacgactt ttgctggccc gaag
//
LOCUS       DBBBSB010000377          705 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.387, whole genome shotgun sequence.
ACCESSION   DBBBSB010000377 DBBBSB010000000
VERSION     DBBBSB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.387"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..444)
                     /locus_tag="ACIUS0_004630"
     CDS             complement(<1..444)
                     /locus_tag="ACIUS0_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGB0222024.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDD"
BASE COUNT          215 a          172 c          131 g          187 t
ORIGIN      
        1 atcatctgga taatcggcat gagcaataac atgcttgccg gtgcgactgt gtacttcaag
       61 aataaagggc aaatgctgac atttttccag cgccgttttc gcttgtttct gttgattatc
      121 tgccagcgca ataaaccagt cgccgccatt catcaaccac aaagacatct gctggaatgc
      181 cagcgcatcc atcgccatct gttcatgatt gcctcttacc gcacgaaccc aatgttgttc
      241 cagtaactgc agacaacgta aactttgcgg cccacgatcg ataacgtctc ccactgagat
      301 aagtaaatct cgccacggat caaaacgaca atgccataat ttgcggcgca actgctcaag
      361 acaaccgtgt atatcgccag aaagccagat atgtcgccat tgatgacccg caattctctg
      421 ataaacgggc gcaggctgtt tcatcaatat tttcctcccg cgctaaagat cacataatct
      481 taacaagaat gttaaaaaac gctggactca gacagtagag tgtgtgttat ggttgactat
      541 aaagtcagcg aaggaaatgc ttctggcttt taacagataa aaagagaccg aacatgattc
      601 ctgttttcgt caacaaacaa taaaaccctt taaaattaat gcgttgaata aatttacgtt
      661 catcttttaa tccctgacac gtaccattgc atattaatac attca
//
LOCUS       DBBBSB010000378          694 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.388, whole genome shotgun sequence.
ACCESSION   DBBBSB010000378 DBBBSB010000000
VERSION     DBBBSB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.388"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            32..493
                     /locus_tag="ACIUS0_004631"
     CDS             32..493
                     /locus_tag="ACIUS0_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGB0222025.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>694)
                     /locus_tag="ACIUS0_004632"
     CDS             complement(534..>694)
                     /locus_tag="ACIUS0_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGB0222026.1"
                     /translation="LAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLA
                     ARQHVKKS"
BASE COUNT          204 a          151 c          159 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc tgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaa
//
LOCUS       DBBBSB010000379          694 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.389, whole genome shotgun sequence.
ACCESSION   DBBBSB010000379 DBBBSB010000000
VERSION     DBBBSB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.389"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..525
                     /locus_tag="ACIUS0_004633"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACIUS0_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>694
                     /locus_tag="ACIUS0_004634"
     CDS             589..>694
                     /locus_tag="ACIUS0_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGB0222027.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          194 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acca
//
LOCUS       DBBBSB010000380          685 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.390, whole genome shotgun sequence.
ACCESSION   DBBBSB010000380 DBBBSB010000000
VERSION     DBBBSB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.390"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            52..685
                     /locus_tag="ACIUS0_004635"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACIUS0_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DBBBSB010000381          683 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.391, whole genome shotgun sequence.
ACCESSION   DBBBSB010000381 DBBBSB010000000
VERSION     DBBBSB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.391"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..570
                     /locus_tag="ACIUS0_004636"
     CDS             <1..570
                     /locus_tag="ACIUS0_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HGB0222028.1"
                     /translation="HNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVIDY
                     VWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKAT
                     LPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTVE
                     ILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          210 a          138 c          157 g          178 t
ORIGIN      
        1 cacaataata gaaatgtgat ttttctggtt ccccatggct ggagcgtgga tattcctgca
       61 atgttgctgg cagctcaggg aaaaaaaatg gccgccatgt ttcatcagca acgaaatcca
      121 gtgattgatt atgtctggaa ttcagtacgg cgtaaattcg gggggcgctt acatgcccgg
      181 gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg atattacctc
      241 ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc gacctataaa
      301 gcgacattac caatcattgg acgtctgatg aacatcagtc aggctatgat tataccactt
      361 ttcccggttt atgatgaaaa aaaacatcta ctgactattg aaattcggcc accaatggat
      421 gcttgcattg ccagcgcgga caataaaacg attgcccgac aaatgaacaa aacagtggaa
      481 attttggtgg ggccacatcc ggaacagtat gtctgggttt taaaattgtt aaaaacacgc
      541 aaatcaaacg aagcggaccc gtacccttga aattataaag tacaagtatt tccactagtt
      601 gtttgtaagc gtacagcctg aaccgtctgg tcagaatctg acgaattaga caaagtggtg
      661 tccaccaaat aagtagtggg aac
//
LOCUS       DBBBSB010000382          680 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.392, whole genome shotgun sequence.
ACCESSION   DBBBSB010000382 DBBBSB010000000
VERSION     DBBBSB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.392"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..204
                     /locus_tag="ACIUS0_004637"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACIUS0_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(270..635)
                     /gene="istB"
                     /locus_tag="ACIUS0_004638"
                     /pseudo
     CDS             complement(270..635)
                     /gene="istB"
                     /locus_tag="ACIUS0_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT          171 a          156 c          170 g          183 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtcaacgga gcaccgtatt
      661 gacgcttatt tattggtgag
//
LOCUS       DBBBSB010000383          679 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.393, whole genome shotgun sequence.
ACCESSION   DBBBSB010000383 DBBBSB010000000
VERSION     DBBBSB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.393"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..251)
                     /locus_tag="ACIUS0_004639"
     CDS             complement(<1..251)
                     /locus_tag="ACIUS0_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGB0222029.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>679)
                     /locus_tag="ACIUS0_004640"
     CDS             complement(272..>679)
                     /locus_tag="ACIUS0_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0222030.1"
                     /translation="HRALSEHKTLSVSWSHYRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
BASE COUNT          155 a          156 c          152 g          216 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gtacttcggt aatgactcca acttactgat agtgttttat
      661 gttcagataa tgcccgatg
//
LOCUS       DBBBSB010000384          670 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.394, whole genome shotgun sequence.
ACCESSION   DBBBSB010000384 DBBBSB010000000
VERSION     DBBBSB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.394"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..141
                     /locus_tag="ACIUS0_004641"
     CDS             <1..141
                     /locus_tag="ACIUS0_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGB0222031.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            256..>670
                     /locus_tag="ACIUS0_004642"
     CDS             256..>670
                     /locus_tag="ACIUS0_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HGB0222032.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          170 a          179 c          174 g          147 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc
      181 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
      241 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
      301 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
      361 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
      421 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
      481 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
      541 gtaaaacatt ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg
      601 gcaataaaaa tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg
      661 ttgccgtctg
//
LOCUS       DBBBSB010000385          668 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.395, whole genome shotgun sequence.
ACCESSION   DBBBSB010000385 DBBBSB010000000
VERSION     DBBBSB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.395"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>668
                     /locus_tag="ACIUS0_004643"
     CDS             <1..>668
                     /locus_tag="ACIUS0_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HGB0222033.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DBBBSB010000386          665 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.396, whole genome shotgun sequence.
ACCESSION   DBBBSB010000386 DBBBSB010000000
VERSION     DBBBSB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.396"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(160..>665)
                     /locus_tag="ACIUS0_004644"
     CDS             complement(160..>665)
                     /locus_tag="ACIUS0_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGB0222034.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          175 a          165 c          146 g          179 t
ORIGIN      
        1 caccagcgcg tttaacgccc tgtgccattt ttcaggcaca aaaaaacccg cataaagcgg
       61 gttctttcaa gtgtccatgt ctgctattcg cctcgcgata cagctttgcg aagcttaccg
      121 taattgaagc agtttatgtg caaaaatgca agaacttttt taaagctgca tcaacctttc
      181 caccagttta tctctgcgaa caacaaacca accattggct ctcgccagtt ccagccatga
      241 ctcaagggaa ataacaatat catcatcccg taactgaatt gtggaaacag tgacaccgcc
      301 tctctgataa cagagaactc gcgtgtcgta acttttctgg catgaaactg gcgttgacgg
      361 atccctttga ctgaaatagc agtcttccag tttttcgaac acatcccacg cctgatcggt
      421 ttcgagcatt ttgccgtggc gtgctgcgcc tcgttctgtc cagaggatga gggagcgggc
      481 tttgggtgaa actggatttt gtgagtagtt taaagcgacc cgcaattctt taaggtcatt
      541 accaacaact ttgaaaaagt gtttcccttc aacgaagcgt actttgttct catgatgatt
      601 ctggcgaata cgcaccggct cagtgccgta aagctgcgcc aaaagttcgg tggtaataac
      661 aggaa
//
LOCUS       DBBBSB010000387          656 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.397, whole genome shotgun sequence.
ACCESSION   DBBBSB010000387 DBBBSB010000000
VERSION     DBBBSB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.397"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>656
                     /locus_tag="ACIUS0_004645"
     CDS             <1..>656
                     /locus_tag="ACIUS0_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222035.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DBBBSB010000388          652 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.398, whole genome shotgun sequence.
ACCESSION   DBBBSB010000388 DBBBSB010000000
VERSION     DBBBSB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.398"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(30..398)
                     /locus_tag="ACIUS0_004646"
                     /pseudo
     CDS             complement(30..398)
                     /locus_tag="ACIUS0_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          194 a          114 c          145 g          199 t
ORIGIN      
        1 gttgtctgga gattcagggg gccagtctaa tcagttgtca tgcactcgga ataaatcata
       61 ccgtgaatag tgctgttttt gattgtcaga ctattggcgt tggtattggt agaaataccg
      121 tcgtccagat agtaggtaga aatcacgccg ttaacggtag cgttattaat gaccgggtag
      181 atatcgccgt tgtaatagct atcagcggca tagttgttcc agcctacata accatcatag
      241 taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta agtttgacca
      301 gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca agccaatgct
      361 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
      421 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
      481 ttatgctgcg ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca
      541 tatataattt atggagaaaa acacgcatca aattgataaa aggacgatat ttcagtatgt
      601 tgcagataat gttatttttg atgcatacgc gttataaata tcaaaaatac aa
//
LOCUS       DBBBSB010000389          649 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.399, whole genome shotgun sequence.
ACCESSION   DBBBSB010000389 DBBBSB010000000
VERSION     DBBBSB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.399"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(24..>649)
                     /locus_tag="ACIUS0_004647"
     rRNA            complement(24..>649)
                     /locus_tag="ACIUS0_004647"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DBBBSB010000390          641 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.400, whole genome shotgun sequence.
ACCESSION   DBBBSB010000390 DBBBSB010000000
VERSION     DBBBSB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.400"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACIUS0_004648"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACIUS0_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HGB0222036.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACIUS0_004649"
     CDS             complement(202..567)
                     /gene="csgA"
                     /locus_tag="ACIUS0_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGB0222037.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          166 a          146 c          155 g          174 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta ctgaggtgta ctggcaatag cggacactac catttgttct tttttaagca
      601 gccatctgat gatatttttc cctgaaggct gccggggaga t
//
LOCUS       DBBBSB010000391          631 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.401, whole genome shotgun sequence.
ACCESSION   DBBBSB010000391 DBBBSB010000000
VERSION     DBBBSB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.401"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>631)
                     /locus_tag="ACIUS0_004650"
     CDS             complement(<1..>631)
                     /locus_tag="ACIUS0_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGB0222038.1"
                     /translation="STKNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLD
                     YQFSTNEIAAVVAERDIEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKA
                     IEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDT
                     VFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYC"
BASE COUNT          199 a          121 c           98 g          213 t
ORIGIN      
        1 cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta ctccacggag
       61 aagctgcttt ggcgatcaaa aaactacgtg aaaccggata atcagcccac tcatgccagg
      121 gttttgtagg tcccacatag tgtataaaca cggtgtcatc attgactgga ttatcaactt
      181 tgtcttttaa ttcatagtta atactatatc gggtattata tttctccgaa ataaacttca
      241 ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt ttacttaccc
      301 aatctgggtc acgcaacatt tcaatagctt ttgacgaaat gttatttaga ttccactcat
      361 caatatttat cagtaaaaaa ccagcattga aatatccaga agctaactgt ggtgtagtta
      421 ataccgaggc tcgattttgc caccattcaa tatctctttc agctacaact gcggcaattt
      481 cattagtaga aaattgataa tctaagagtt ctttaatact acccttgcaa gcaatatctg
      541 catcaagata tagtattttt tcatgtttat gatagaaata atctgcaatt ataaatcgaa
      601 aatatgttgc gtaagtccag tttttcgtgc t
//
LOCUS       DBBBSB010000392          604 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.402, whole genome shotgun sequence.
ACCESSION   DBBBSB010000392 DBBBSB010000000
VERSION     DBBBSB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.402"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..343)
                     /locus_tag="ACIUS0_004651"
     CDS             complement(<1..343)
                     /locus_tag="ACIUS0_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222039.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT          196 a          123 c           94 g          191 t
ORIGIN      
        1 ggagaatgtt atcattgcta caccctgtgc ttaataactg caactctccg ccgttcagcg
       61 atgctttgat aattctgttc ccatagatat cgttatcaaa ataaagcgta aatatcatat
      121 ttccgtaact gttatcatct ctttttttgc acgtccgaat aaacgcattc agtaaattac
      181 tctctctaag aaaataaata tcttcattgt tgttaatgac attaataacg tcgttcttaa
      241 aatcctcaaa gtcttgttgc ggtaacttct ggttacgctg cttactatca gcaagcggtg
      301 aatccggacg acaacaacat aggttttgta taacccccag cattcttttc acctccgtta
      361 acagtatatt cacgacactg aaacgagaga atgctgataa ttttctcggc gagatatcaa
      421 aaatcgctca ttataaaaca agtaacttca acgtcgcgta accgtggacg ataaaaaaac
      481 aataatgtca ttcatattta aataatttag ccttaaatga tttaaataat attagccacc
      541 tgataaatac atttttaatg atagagaggg tcaacaggct tgcggaagtc gtcacatctt
      601 catg
//
LOCUS       DBBBSB010000393          602 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.403, whole genome shotgun sequence.
ACCESSION   DBBBSB010000393 DBBBSB010000000
VERSION     DBBBSB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.403"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            18..>602
                     /locus_tag="ACIUS0_004652"
     CDS             18..>602
                     /locus_tag="ACIUS0_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HGB0222040.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          162 c          174 g          136 t
ORIGIN      
        1 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
       61 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      121 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      181 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      241 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      301 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      361 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      421 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      481 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      541 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      601 gg
//
LOCUS       DBBBSB010000394          594 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.404, whole genome shotgun sequence.
ACCESSION   DBBBSB010000394 DBBBSB010000000
VERSION     DBBBSB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.404"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            490..>594
                     /locus_tag="ACIUS0_004653"
     CDS             490..>594
                     /locus_tag="ACIUS0_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222041.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKN"
BASE COUNT          218 a          105 c          121 g          150 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 ggggggggga tgaaaatacc tgaagcagta aatcatatta atgttcagaa taatattgat
      541 cttgttgatg gaaaaacgaa tccgaataaa gcaacaaaag cattacaaaa aaat
//
LOCUS       DBBBSB010000395          566 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.405, whole genome shotgun sequence.
ACCESSION   DBBBSB010000395 DBBBSB010000000
VERSION     DBBBSB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.405"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACIUS0_004654"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACIUS0_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGB0222042.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACIUS0_004655"
     CDS             complement(405..>566)
                     /locus_tag="ACIUS0_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGB0222043.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DBBBSB010000396          555 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.406, whole genome shotgun sequence.
ACCESSION   DBBBSB010000396 DBBBSB010000000
VERSION     DBBBSB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.406"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>555
                     /locus_tag="ACIUS0_004656"
     CDS             <1..>555
                     /locus_tag="ACIUS0_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGB0222044.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETV"
BASE COUNT          164 a          135 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcag
//
LOCUS       DBBBSB010000397          553 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.407, whole genome shotgun sequence.
ACCESSION   DBBBSB010000397 DBBBSB010000000
VERSION     DBBBSB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.407"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>553)
                     /locus_tag="ACIUS0_004657"
     CDS             complement(<1..>553)
                     /locus_tag="ACIUS0_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HGB0222045.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          119 c          135 g          165 t
ORIGIN      
        1 ccatttaata tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc
       61 ttcctggacg atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc
      121 aagtgccgtg agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat
      181 ctgactgatc gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa
      241 tgtggctgtt ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa
      301 tttgtctgct tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac
      361 aacttcgggc ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt
      421 atttttgtca gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga
      481 taagtccgct accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt
      541 cgcttgctcc aca
//
LOCUS       DBBBSB010000398          536 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.408, whole genome shotgun sequence.
ACCESSION   DBBBSB010000398 DBBBSB010000000
VERSION     DBBBSB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.408"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>536)
                     /gene="rfbG"
                     /locus_tag="ACIUS0_004658"
     CDS             complement(<1..>536)
                     /gene="rfbG"
                     /locus_tag="ACIUS0_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HGB0222046.1"
                     /translation="IWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQ
                     TQFDYFFEAAGPGCTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSW
                     YIDRKPTMLYRQHENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNN
                     QLGKGYIGNLILALSFWK"
BASE COUNT          166 a          108 c           67 g          195 t
ORIGIN      
        1 acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat
       61 tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt cgataccact
      121 tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt
      181 ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat
      241 ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat
      301 ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg
      361 tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat
      421 caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg
      481 cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac caaata
//
LOCUS       DBBBSB010000399          519 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.409, whole genome shotgun sequence.
ACCESSION   DBBBSB010000399 DBBBSB010000000
VERSION     DBBBSB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.409"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..147
                     /locus_tag="ACIUS0_004659"
     CDS             <1..147
                     /locus_tag="ACIUS0_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222047.1"
                     /translation="VNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTYNYDL
                     LNAI"
BASE COUNT          163 a          117 c          103 g          136 t
ORIGIN      
        1 gttaataccc tcgagaaaga cgaaaattct ccggacaatg gctttgaggt cggggaatat
       61 aacacatatg aagcatataa ctcagagaat caatatttta ccagagagga ctatacgtat
      121 aattacgacc ttttaaatgc aatatagact taataacttc ttacaaagtc tattattaat
      181 accgtagata ttcatcttac cctgtgcacc aaaagcaaaa acttatcgac gcgggtttga
      241 aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct
      301 gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc
      361 atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg
      421 tgaaactgac cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt
      481 tctgcccctc caaaagcaaa aacccgccga agcgggttt
//
LOCUS       DBBBSB010000400          519 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.410, whole genome shotgun sequence.
ACCESSION   DBBBSB010000400 DBBBSB010000000
VERSION     DBBBSB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.410"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            368..>519
                     /locus_tag="ACIUS0_004660"
     CDS             368..>519
                     /locus_tag="ACIUS0_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGB0222048.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          137 c          113 g          140 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatggt ctctctatag gtctctctag ggtctcctct
       61 aggcattacc cccagaagct aactctatgg ccctctatgg tcctctatgg ccctgtacgt
      121 cattctttag gatgccctgt agtttcgtat agcctgatgc ctagagagac cctagagaga
      181 ccctagagtg gctcctagag agaccctccc agtagctccg cacagctaca tcgtcatcat
      241 catagtattc aatctcaaag tcattctgtt tgcatcctgt tgtcctgctg gtctcccttc
      301 gcttggggac tttgagtagg tcagtggggg tgcgacttca gggtgcaaac aaaaaggaaa
      361 tcaagcgatg tctcaacaac cacaacaacc acaacaagaa atcagcatca ttaacctcga
      421 ccagcttgtg tccatgacct cagttgaaat tgctgagctg acaggcaagg agcaccgaaa
      481 tgtccttcgt gatattcgta agatgctcga agaacttaa
//
LOCUS       DBBBSB010000401          511 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.411, whole genome shotgun sequence.
ACCESSION   DBBBSB010000401 DBBBSB010000000
VERSION     DBBBSB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.411"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..511
                     /locus_tag="ACIUS0_004661"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACIUS0_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DBBBSB010000402          501 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.412, whole genome shotgun sequence.
ACCESSION   DBBBSB010000402 DBBBSB010000000
VERSION     DBBBSB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.412"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            320..>501
                     /locus_tag="ACIUS0_004662"
     CDS             320..>501
                     /locus_tag="ACIUS0_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGB0222049.1"
                     /translation="MKTLSQNTTSSACAPETDLQQLVATLVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRL"
BASE COUNT          101 a          152 c          124 g          124 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaaacatt gtctcagaac accacctctt cggcctgtgc
      361 accggagact gacctgcaac aactggttgc caccctcgtc cctgatgaac agcgcatcag
      421 cttctggccg cagcactttg gcctcattcc acagtgggtg accctggagc cccgtgtctt
      481 cggctggatg gaccgtctgt g
//
LOCUS       DBBBSB010000403          498 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.413, whole genome shotgun sequence.
ACCESSION   DBBBSB010000403 DBBBSB010000000
VERSION     DBBBSB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.413"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..429
                     /locus_tag="ACIUS0_004663"
     CDS             <1..429
                     /locus_tag="ACIUS0_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGB0222050.1"
                     /translation="GVKDVYTCEIVGYAMGERMMKELTGKALFMALRSQRLPAGLIHH
                     TDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFKSRD
                     EAISVIREYIEIFYNRQRRHSRLGNISPAAFRIKYYQMTA"
BASE COUNT          137 a          116 c          133 g          112 t
ORIGIN      
        1 ggcgtcaaag atgtttatac gtgcgaaatt gtcggttacg ccatgggaga gcgcatgatg
       61 aaagagttga cgggtaaagc cctgtttatg gcgctcagga gccagcgcct acctgccggg
      121 ctaatccacc acaccgatcg aggttcacag tactgcgcat acgattacag ggtcatacag
      181 gagcagtctg gtctgaaaac atcaatgtcg cgtaaaggta actgttacga caacgctccg
      241 atggaaagct tctggggaac gctgaaaaat gaaagcctga gccactatcg ttttaagagc
      301 cgggatgagg ccatctcagt aatacgggaa tacattgaga ttttctacaa tcgtcagcgt
      361 cgtcactctc gtctggggaa tatctccccg gcagccttca ggataaaata ttatcagatg
      421 actgcttaga aaaagaacaa atggtggtgt ccgctattgc cagtacacct caggccatgt
      481 gcgcggtaac tgaccggg
//
LOCUS       DBBBSB010000404          486 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.414, whole genome shotgun sequence.
ACCESSION   DBBBSB010000404 DBBBSB010000000
VERSION     DBBBSB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.414"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
BASE COUNT          159 a           73 c           96 g          158 t
ORIGIN      
        1 tttcctgcta aaaatagccc tgctttacgg atggttgtat tgcaataaca cgaaagtagt
       61 aagagattaa aggtatctgt cagcgataac tctgcaagtg atgacatttt gtaaaccaaa
      121 taatgtaaat cagcaatgta tcttttcact taattataac aaatgtggta ttcactaaga
      181 atctgatacg agcttacatc acgactattt tagtctcatc gcgggataca gggatgcacc
      241 gatggatctc agcacaccgt aatgcaatac ctgaacaaat agaacagtaa agtggttcag
      301 tattaatgaa agctcttatt ttccgttggg aaaacttaaa atagatgaga aggctttttg
      361 atttcttatg tgatctcatt tcgagtattg agaaatttgt gatttttaaa tttttcaata
      421 agccctgtaa gggattgggg ttttatgaga aataaagaga cgtattgtct ttgtttaaac
      481 ggtacg
//
LOCUS       DBBBSB010000405          484 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.415, whole genome shotgun sequence.
ACCESSION   DBBBSB010000405 DBBBSB010000000
VERSION     DBBBSB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.415"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..484
                     /locus_tag="ACIUS0_004664"
                     /pseudo
     CDS             1..484
                     /locus_tag="ACIUS0_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DBBBSB010000406          473 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.416, whole genome shotgun sequence.
ACCESSION   DBBBSB010000406 DBBBSB010000000
VERSION     DBBBSB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.416"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..473
                     /locus_tag="ACIUS0_004665"
                     /pseudo
     CDS             1..473
                     /locus_tag="ACIUS0_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DBBBSB010000407          472 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.417, whole genome shotgun sequence.
ACCESSION   DBBBSB010000407 DBBBSB010000000
VERSION     DBBBSB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.417"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            3..>472
                     /locus_tag="ACIUS0_004666"
     CDS             3..>472
                     /locus_tag="ACIUS0_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HGB0222051.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DBBBSB010000408          472 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.418, whole genome shotgun sequence.
ACCESSION   DBBBSB010000408 DBBBSB010000000
VERSION     DBBBSB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.418"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..281)
                     /locus_tag="ACIUS0_004667"
                     /pseudo
     CDS             complement(1..281)
                     /locus_tag="ACIUS0_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(282..>472)
                     /locus_tag="ACIUS0_004668"
     CDS             complement(282..>472)
                     /locus_tag="ACIUS0_004668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222052.1"
                     /translation="HLFENLRRKGHHCHGLTHLSWAERKIFYRVLGKKFCGLFIYPGQ
                     SVRQVVAVYAPGDRGLNR"
BASE COUNT           84 a          153 c          136 g           99 t
ORIGIN      
        1 ttgttgttct cctgcttatg ccttttccgg gcgtccccct ggcggggcgg gctgtcgtga
       61 acggagccag cgaagctgtc tccgccctgg cgggcttcca tccctgacgc aaaaacccgg
      121 gtttgcagcg accggaacgg tcgcgccacc cttgtcgcgc cgtggcctgc gctcccggca
      181 tcccggcaaa agacgccggg atgtaaggag caacgccgtt tcgcaaaccg cgcttcacgt
      241 cgttgcggcg ggtccctgcc cgttgttctg tccggtgcca ttcacctgtt taagcccctg
      301 tcgccggggg catagacagc cacaacctgc cgcactgact gtccagggta aatgaacagc
      361 ccacaaaatt ttttacccaa aacccggtaa aaaattttgc gttctgccca ggacaggtgc
      421 gtcaggccgt ggcagtgatg ccccttgcgg cgaaggttct caaacaggtg at
//
LOCUS       DBBBSB010000409          471 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.419, whole genome shotgun sequence.
ACCESSION   DBBBSB010000409 DBBBSB010000000
VERSION     DBBBSB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.419"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..139
                     /locus_tag="ACIUS0_004669"
     CDS             <1..139
                     /locus_tag="ACIUS0_004669"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0222053.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..471
                     /locus_tag="ACIUS0_004670"
                     /pseudo
     CDS             172..471
                     /locus_tag="ACIUS0_004670"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          104 c          115 g          113 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt g
//
LOCUS       DBBBSB010000410          465 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.420, whole genome shotgun sequence.
ACCESSION   DBBBSB010000410 DBBBSB010000000
VERSION     DBBBSB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.420"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>465
                     /locus_tag="ACIUS0_004671"
     CDS             <1..>465
                     /locus_tag="ACIUS0_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGB0222054.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DBBBSB010000411          463 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.421, whole genome shotgun sequence.
ACCESSION   DBBBSB010000411 DBBBSB010000000
VERSION     DBBBSB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.421"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>463
                     /locus_tag="ACIUS0_004672"
     CDS             <1..>463
                     /locus_tag="ACIUS0_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGB0222055.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DBBBSB010000412          459 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.422, whole genome shotgun sequence.
ACCESSION   DBBBSB010000412 DBBBSB010000000
VERSION     DBBBSB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.422"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>459)
                     /locus_tag="ACIUS0_004673"
     CDS             complement(<1..>459)
                     /locus_tag="ACIUS0_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HGB0222056.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DBBBSB010000413          448 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.423, whole genome shotgun sequence.
ACCESSION   DBBBSB010000413 DBBBSB010000000
VERSION     DBBBSB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.423"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..382)
                     /locus_tag="ACIUS0_004674"
     CDS             complement(<1..382)
                     /locus_tag="ACIUS0_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222057.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLL"
BASE COUNT          135 a          106 c           96 g          111 t
ORIGIN      
        1 ttaacagact tgattcatcc tcacaaaata taagtttttc ctggaatgcc catgtctcat
       61 tgaggttcca gatatcaaat tcgaagtcgt gataacgaaa acggatccca ccaaacttat
      121 taaaacgcag ttgtttgacg ggcagttcag ccaatgtctg gaagagttcc tcacgggagc
      181 gaccgatgac gatatcaagg tctgaagaga atccatcgac gccatacaag cctaagtcac
      241 gcaccatccc gccaaaaatc ctggccggac aggaacaggc cagagtatca agtaatactc
      301 tggctttctt atgctcatca ctaagcgtaa aaaaatcggc gatacgctgt tcaagcagtt
      361 gggtgtttga gaccacattc atacagtgat tttagctcat cgtgaaggcg cattaaataa
      421 ctatccgtca tgcctgagat tgcgtcgg
//
LOCUS       DBBBSB010000414          443 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.424, whole genome shotgun sequence.
ACCESSION   DBBBSB010000414 DBBBSB010000000
VERSION     DBBBSB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.424"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            213..>443
                     /locus_tag="ACIUS0_004675"
     CDS             213..>443
                     /locus_tag="ACIUS0_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGB0222058.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DBBBSB010000415          442 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.425, whole genome shotgun sequence.
ACCESSION   DBBBSB010000415 DBBBSB010000000
VERSION     DBBBSB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.425"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            43..>442
                     /locus_tag="ACIUS0_004676"
     CDS             43..>442
                     /locus_tag="ACIUS0_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HGB0222059.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPR"
BASE COUNT           99 a          117 c          120 g          106 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccgcg at
//
LOCUS       DBBBSB010000416          434 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.426, whole genome shotgun sequence.
ACCESSION   DBBBSB010000416 DBBBSB010000000
VERSION     DBBBSB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.426"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            35..434
                     /locus_tag="ACIUS0_004677"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACIUS0_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DBBBSB010000417          432 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.427, whole genome shotgun sequence.
ACCESSION   DBBBSB010000417 DBBBSB010000000
VERSION     DBBBSB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.427"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            16..432
                     /locus_tag="ACIUS0_004678"
                     /pseudo
     CDS             16..432
                     /locus_tag="ACIUS0_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           93 a          122 c          118 g           99 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
      241 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
      421 ttgcgcacgt at
//
LOCUS       DBBBSB010000418          406 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.428, whole genome shotgun sequence.
ACCESSION   DBBBSB010000418 DBBBSB010000000
VERSION     DBBBSB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.428"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..406
                     /locus_tag="ACIUS0_004679"
                     /pseudo
     CDS             1..406
                     /locus_tag="ACIUS0_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          111 c          119 g           76 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaa
//
LOCUS       DBBBSB010000419          404 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.429, whole genome shotgun sequence.
ACCESSION   DBBBSB010000419 DBBBSB010000000
VERSION     DBBBSB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.429"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
BASE COUNT          130 a           72 c           83 g          119 t
ORIGIN      
        1 gaacttcagc acttttttat tataaaagga caaacaaaat tggctgtaaa aaaacgatac
       61 tgtttcagtt aactgtgaat agcttgcttg caccgtattg ttcttgtttt tcggttagtt
      121 aaacatcgta cttcatattt gaacgttctg ccggaatgca ttatcaatag aggtaaagtc
      181 gcaaccccaa atcgtaaagg aaaccgtagc acgtcgtatg caagaacgtg ccacggctgg
      241 ctgatggatg ttcgatagcg cgagtttgaa tgaaaatcag ccggagatga ttttacataa
      301 ttgctacgga attattcaat acaggaattg cttgcgtctg catggattga cctgaaatat
      361 tcccgaaaat ttctctaaaa aactcgaaaa aaatggtaac tggt
//
LOCUS       DBBBSB010000420          391 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.430, whole genome shotgun sequence.
ACCESSION   DBBBSB010000420 DBBBSB010000000
VERSION     DBBBSB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.430"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..361
                     /locus_tag="ACIUS0_004680"
     CDS             <1..361
                     /locus_tag="ACIUS0_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGB0222060.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLADSVMTPTY"
BASE COUNT          107 a           89 c           89 g          106 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcggattcg gtaatgactc caacttactg
      361 atagtgtttt atgttcagat aatgcccgat g
//
LOCUS       DBBBSB010000421          390 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.431, whole genome shotgun sequence.
ACCESSION   DBBBSB010000421 DBBBSB010000000
VERSION     DBBBSB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.431"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(42..>390)
                     /locus_tag="ACIUS0_004681"
     CDS             complement(42..>390)
                     /locus_tag="ACIUS0_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGB0222061.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DBBBSB010000422          381 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.432, whole genome shotgun sequence.
ACCESSION   DBBBSB010000422 DBBBSB010000000
VERSION     DBBBSB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.432"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(273..>381)
                     /locus_tag="ACIUS0_004682"
     CDS             complement(273..>381)
                     /locus_tag="ACIUS0_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HGB0222062.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          106 a           87 c           77 g          111 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt t
//
LOCUS       DBBBSB010000423          378 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.433, whole genome shotgun sequence.
ACCESSION   DBBBSB010000423 DBBBSB010000000
VERSION     DBBBSB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.433"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..246)
                     /locus_tag="ACIUS0_004683"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="ACIUS0_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           87 a          120 c           86 g           85 t
ORIGIN      
        1 gtctccgtta tggcgtacac cacatcttcc agtaatcgcc gcttcaccgg actcaggacc
       61 ccgtatcacc atccggaacc ccttatccgc cacatgccag gagaaccttt ccataaactc
      121 gtcgccactc atcacaaggt tttcttctcg ttttgtccgg tggctgttgt aacgcaggcc
      181 aatttcatcc tgaccggcat aatgttccag gcggctcatc tgcaccggct gcttctccag
      241 ccagttaagc catcggtttc cctgctgaaa aatatcggca aaacggggaa gcatcagaaa
      301 ggcggaataa cgccatccgg ccagtgaacc gtaccgcatt cagggcaata catgccgtcg
      361 gcgttgatac cggcagga
//
LOCUS       DBBBSB010000424          375 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.434, whole genome shotgun sequence.
ACCESSION   DBBBSB010000424 DBBBSB010000000
VERSION     DBBBSB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.434"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>375
                     /locus_tag="ACIUS0_004684"
     CDS             <1..>375
                     /locus_tag="ACIUS0_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGB0222063.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DBBBSB010000425          372 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.435, whole genome shotgun sequence.
ACCESSION   DBBBSB010000425 DBBBSB010000000
VERSION     DBBBSB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.435"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..311)
                     /locus_tag="ACIUS0_004685"
     CDS             complement(<1..311)
                     /locus_tag="ACIUS0_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGB0222064.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DBBBSB010000426          360 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.denovo.436, whole genome shotgun sequence.
ACCESSION   DBBBSB010000426 DBBBSB010000000
VERSION     DBBBSB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.denovo.436"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..359)
                     /locus_tag="ACIUS0_004686"
     CDS             complement(<1..359)
                     /locus_tag="ACIUS0_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222065.1"
                     /translation="MQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQCDCDEIA
                     ESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRIRDINEN
                     FVGMDFTCEFRPEKMTN"
BASE COUNT          114 a           75 c           56 g          115 t
ORIGIN      
        1 aaattcgtca ttttttcggg tcggaattca caggtaaaat ccatcccaac aaaattctca
       61 ttaatatctc ttatccgaaa ctcactgcca taaagattat taattatcac tctatattta
      121 acattgtcct gcagatacgt atcaaaacta agtgctggcg ctcctagctt tgaggcatca
      181 tagttaatgt tagacatata ggcaatcata cgattgaagt tcgactctgc tatttcatcg
      241 cagtcacatt gacccgttga tataaagaaa gtgagtgccg ccatggtaga ctcaagcctt
      301 tctttaaaag agtcgaaagc caggctttta atatttaaat caccataatt actctgtatt
//
LOCUS       DBBBSB010000427         9932 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.140, whole genome shotgun sequence.
ACCESSION   DBBBSB010000427 DBBBSB010000000
VERSION     DBBBSB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.140"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            110..1015
                     /locus_tag="ACIUS0_004687"
     CDS             110..1015
                     /locus_tag="ACIUS0_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HGB0222066.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACIUS0_004688"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACIUS0_004688"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HGB0222067.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACIUS0_004689"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACIUS0_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HGB0222068.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="ACIUS0_004690"
     CDS             complement(3327..4091)
                     /locus_tag="ACIUS0_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HGB0222069.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4318..4623)
                     /locus_tag="ACIUS0_004691"
     CDS             complement(4318..4623)
                     /locus_tag="ACIUS0_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGB0222070.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACIUS0_004692"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACIUS0_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HGB0222071.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="ACIUS0_004693"
     CDS             complement(5995..6198)
                     /locus_tag="ACIUS0_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGB0222072.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACIUS0_004694"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACIUS0_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HGB0222073.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7506)
                     /locus_tag="ACIUS0_004695"
     CDS             complement(7159..7506)
                     /locus_tag="ACIUS0_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HGB0222074.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7712..8500)
                     /locus_tag="ACIUS0_004696"
     CDS             complement(7712..8500)
                     /locus_tag="ACIUS0_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HGB0222075.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACIUS0_004697"
     CDS             complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACIUS0_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HGB0222076.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            9262..9891
                     /gene="intI1"
                     /locus_tag="ACIUS0_004698"
                     /pseudo
     CDS             9262..9891
                     /gene="intI1"
                     /locus_tag="ACIUS0_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
BASE COUNT         2084 a         2919 c         2863 g         2066 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     7441 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     7501 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7561 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcata
     7621 gttgagcggc gggcgcagcc cgtccgcttg aacgccgagt taggcatcag atgccctcgg
     7681 cgcgggtcga tgcacttttc gcacatgccg ctcaacgcaa gattctctca atcgttgctt
     7741 tggcatatcg aacgaacgcg gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc
     7801 tacccaggta cgccgcgcgt gccttgcaga tgaggggccg atgctcggca ggcaaacgct
     7861 ccgataccca tgcggcagca acgtccttag gagcaatgag accagttgaa gcgctgtacc
     7921 aaatgcgagc aagagcaaga acgacgttcc gctcgtcacc cttccaatcc gactctgcat
     7981 tccactgggc aatagtgtcg aaaagcgcct tggagaaatg ctccttcggc accggctcga
     8041 aaaacgtggc tgcggatggg cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca
     8101 aaatcgcaag atcgtgatcc agaacggcag gctcgaacgt tccggaaagg atgtcgtggc
     8161 ggagccactc accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc
     8221 ttcgagcgac aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg
     8281 agactttcag caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga
     8341 cggtcacgag caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga
     8401 acaggtggat tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg
     8461 acagctgctt gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca
     8521 gcggcagctt ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg
     8581 cgacttaaaa gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt
     8641 ttccaaatct ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg
     8701 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
     8761 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
     8821 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
     8881 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
     8941 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
     9001 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
     9061 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
     9121 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     9181 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     9241 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     9301 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     9361 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     9421 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     9481 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     9541 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     9601 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     9661 cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg
     9721 cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc
     9781 aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagctgtcg
     9841 cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt tgggcactgt
     9901 tgcaaatagt cggtggtgat aaacttatca tc
//
LOCUS       DBBBSB010000428         9713 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.145, whole genome shotgun sequence.
ACCESSION   DBBBSB010000428 DBBBSB010000000
VERSION     DBBBSB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.145"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(35..205)
                     /locus_tag="ACIUS0_004699"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACIUS0_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACIUS0_004700"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACIUS0_004700"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGB0222077.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACIUS0_004701"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACIUS0_004701"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGB0222078.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACIUS0_004702"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACIUS0_004702"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGB0222079.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACIUS0_004703"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACIUS0_004703"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGB0222080.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACIUS0_004704"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACIUS0_004704"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGB0222081.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /locus_tag="ACIUS0_004705"
     CDS             5100..6233
                     /locus_tag="ACIUS0_004705"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGB0222082.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="ACIUS0_004706"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="ACIUS0_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGB0222083.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACIUS0_004707"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACIUS0_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGB0222084.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACIUS0_004708"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="ACIUS0_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGB0222085.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACIUS0_004709"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="ACIUS0_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGB0222086.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACIUS0_004710"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="ACIUS0_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGB0222087.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..9415
                     /gene="epmB"
                     /locus_tag="ACIUS0_004711"
     CDS             8387..9415
                     /gene="epmB"
                     /locus_tag="ACIUS0_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGB0222088.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2485 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
     8881 cgatccgctg atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat
     8941 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
     9001 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
     9061 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
     9121 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
     9181 aacgttggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
     9241 tgtgctagat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
     9301 gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga
     9361 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
     9421 taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
     9481 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
     9541 tccggcaaga gcaacaaacg caagtacagc aaccactgag aaatttaatc tttgcacgtt
     9601 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     9661 taattaacct ttcttttcgg taatgactcc aacttactga tagtgtttta tgt
//
LOCUS       DBBBSB010000429         7053 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.175, whole genome shotgun sequence.
ACCESSION   DBBBSB010000429 DBBBSB010000000
VERSION     DBBBSB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.175"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            59..1102
                     /locus_tag="ACIUS0_004712"
     CDS             59..1102
                     /locus_tag="ACIUS0_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGB0222089.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALSIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACIUS0_004713"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACIUS0_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACIUS0_004714"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACIUS0_004714"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGB0222090.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACIUS0_004715"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACIUS0_004715"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGB0222091.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACIUS0_004716"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACIUS0_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGB0222092.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="ACIUS0_004717"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="ACIUS0_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGB0222093.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACIUS0_004718"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="ACIUS0_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGB0222094.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1717 a         1734 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gagcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DBBBSB010000430         5385 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.200, whole genome shotgun sequence.
ACCESSION   DBBBSB010000430 DBBBSB010000000
VERSION     DBBBSB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.200"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(50..1065)
                     /locus_tag="ACIUS0_004719"
                     /pseudo
     CDS             complement(50..1065)
                     /locus_tag="ACIUS0_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1460..2116
                     /locus_tag="ACIUS0_004720"
     CDS             1460..2116
                     /locus_tag="ACIUS0_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HGB0222095.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(2435..2863)
                     /locus_tag="ACIUS0_004721"
                     /pseudo
     CDS             complement(2435..2863)
                     /locus_tag="ACIUS0_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2896..4287)
                     /locus_tag="ACIUS0_004722"
     CDS             complement(2896..4287)
                     /locus_tag="ACIUS0_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HGB0222096.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4324..4896)
                     /locus_tag="ACIUS0_004723"
     CDS             complement(4324..4896)
                     /locus_tag="ACIUS0_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGB0222097.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(5033..>5385)
                     /locus_tag="ACIUS0_004724"
     CDS             complement(5033..>5385)
                     /locus_tag="ACIUS0_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HGB0222098.1"
                     /translation="FDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCISGHGMP
                     GATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRLDLAS
                     PVLEPEVIWLGRRR"
BASE COUNT         1269 a         1428 c         1288 g         1400 t
ORIGIN      
        1 tgcttaacgt gagttttcgt tccactgagc gtcagacccc taaaatcctg atgctgatgt
       61 agtcccgttt tatcgtcatc acgaagcttt ctgctatgcc attgctttcc gggcttcgca
      121 ctgctgtcgt acaaggctcc agtcccagca accgggcgaa cgccggcact gttggcaact
      181 gccttccgaa gcgtttttcc accgcaccca gcatgacatc ctgcaccgtt tctttgtcat
      241 agcctccggt gctcgctgcc cagtcaatga tctccctgtc gcagcagtcc tgcgcgaagg
      301 tcacccgcag cttttcgcca ttatcacagc ggaactcaaa gccatctgag caccagcgcc
      361 ggttactttc cgctactgct acgcgcccct tatgggcccg cttacggcaa ggatcagcgg
      421 gtttacgttc aagaagcaga ttatgcgttc tcataatgcg atacacccgc tttgcattaa
      481 ccacaggcag gccgtcccgc tccgactccc gtcgcagcag cgcccacaca cggcgataac
      541 catacgtcgg caggtcagcc accgccatgt ttatccggga caatacggtg gtatcgtcag
      601 aacggggctg ccgtctgcga tcctgtcagt cagatggccg gtgaacccga atgctcaact
      661 gcgcacgcga cacgccaaga cttctgcaaa cgtcgcttat tcgtcgttcc caggcaacga
      721 gggcgcatgc gcaatccatt tttttgcccg gccgaactcc acggcctctt tgagtatctc
      781 ggcttccatc gtctttttgc ccagaaggcg ctggagttcg cgaatttgct tattggcggc
      841 agcgagttcg gaggccggca ccacttcctc gcccgatgca acagccgtca gtgaaccgtc
      901 ttaatattgc ttgcgccatt tgaatatctg gtttgcattg atgccatgca ggcgcgcgac
      961 atgagacacg gtcataccgg gctccatagt ctgctgaata atggcaattt tttcctgcgg
     1021 tgtacgacgc cgacgccgct caggtcctga taacacttca accatcttat cgttctgact
     1081 ggtattaaac atagttccaa gactacctct tattttaaga gagtcgaagt gtctggtgat
     1141 ctatggggcc agtctagccc tcttccaagt gtagggagat atagtcaaag tttgcttctc
     1201 cgccgatgag tccgccctgc gtcggatagt ccgatttttt accggcgaag cacggctctc
     1261 ctaaccctat ctaaagcgaa taactttttt caacagggcc gcgtgccaat gcaagagcga
     1321 taggcgctct acgcgccaat ttgaccactt aaaacaggta aattgagggg ttgtaatgtg
     1381 ttgatgtaac aggcttttat tttaatgtct tggcatatgt ataatggtag tctagccctc
     1441 ctttcaacaa ggagtactca tggaaaccta caatcataca tatcggcacc acaacttttc
     1501 acataaagac ttaagtgatc tcaccttcac cgcttgcaca ttcattcgca gcgactttcg
     1561 acgtgctaac ttgcgtgata cgacattcgt caactgcaag ttcattgaac agggtgatat
     1621 cgaaggctgc cactttgatg tcgcagatct tcgtgatgca agtttccaac aatgccaact
     1681 tgcgatggca aacttcagta atgccaattg ctacggtata gagttccgtg cgtgtgattt
     1741 aaaaggtgcc aacttttccc gaacaaactt tgcccatcaa gtgagtaatc gtatgtactt
     1801 ttgctcagca tttatttctg gatgtaatct ttcctatgcc aatatggaga gggtttgttt
     1861 agaaaaatgt gagttgtttg aaaatcgctg gataggaacg aacctagcgg gtgcatcact
     1921 gaaagagtca gacttaagtc gaggtgtttt ttccgaagat gtctgggggc aatttagcct
     1981 acagggtgcc aatttatgcc acgccgaact cgacggttta gatccccgca aagtcgatac
     2041 atcaggtatc aaaattgcag cctggcagca agaactgatt ctcgaagcac tgggtattgt
     2101 tgtttatcct gactaattgc tttgatgtgt gattttaaac gctcaaattt ataaaacgaa
     2161 taatattgtc aaccttgcaa taatagacat agggacaacc atgtataaat ttttaccctc
     2221 aggcgtcttc acaatagagc catatagcag tgggtcactt agtgggctta atttttccat
     2281 taaagataat attaatattg ctcagtataa aacatcatat ggtagcccat cttggcaagt
     2341 aaacataagg ctgccattta taaggcactg ttgcaaaaat gtggaggtga taggggtact
     2401 acgccggaac cccagatttt tccgtccgtt caaattatcc acggttgagc agcttgatcc
     2461 cgtccgcccg cagttgacca acaggggaaa tatgcctgta caaagtttgc cgggttatcc
     2521 caagttcctg gcatagcgta ctgacctttg tttctgattg tcccattgat gccattgcca
     2581 gtcgcagttt gactggcgtc attttataag gtcggccgcc gttccgccca cgggctcttg
     2641 ctgatgccag acctgcagtc gttctttcag caatcagttc gcgctcaaac tccgccagtg
     2701 cggcaaagat accgaagaca agcttgccag cggccgttgt tgtgtcgata gtcgccccgt
     2761 gaccggtcag cactttcaga ccggtccccc tcgcggtcag gtcgtgcact gtattaatga
     2821 gatgacgaag atcacgaccg aggcggtcca gcttccatac gaccgagagc ataaaaaata
     2881 ttggggcagc gtgagctatg ctgccatcgc tggtcccttc ccgtcacttt gaaaacccgg
     2941 ataccagcag acaaatttat cctgcagttc gttattcaaa acggcggtcc gggtctgtaa
     3001 caaataatga gccccttttc tgctccactg catctgctgc tttttggcca ttcgtctggc
     3061 gatcacttca ttgatcgtgg attccacaaa cgcggttgat accggctctc cgtaccgacg
     3121 catttcgccg tagtttggga tcatcatctt attattccgg atgtaggtat acatttcatc
     3181 cagatgtttt tgcagggatt tcaggctagg atagctgagt tcagggtcat cgcaatacat
     3241 aacgcaatta tcaatatgtt ccagcgcagc gacaacattg ccatgccaga gatatcgttt
     3301 aatgctttcc agcagtgcca gaactttact ccccgcctct ggatctgata ccagcagtcc
     3361 ccgggcatat tgcatgagca ccttcagcct catggtgata tgaaaccagt ccagcacatg
     3421 cgttgactca gggtacatac cgaactggag gtccctgaga ttatccgcgc cgtcggacag
     3481 aaaaaatatc tgctggttgg cctgcattcc ctgcgctgaa aggtgggtca tgagcctgcg
     3541 ttccggatga cagtcatcct tctgaacaaa accgaagcgg cgggtgtcag caggcgcacc
     3601 aacggaaaaa gacttcccgg caataatttc aaaattacgc tttttgtcat cccgatcgcg
     3661 aacataacca ccatctatac ccaccacaag cggttttcct ggcctgggca ggttccccca
     3721 gtcgcggggg cagccggaaa gaaaacctga atgagcttca gcctcagcgt caagacgctg
     3781 cgccacctgg cataaatgat tcctcaccgt tgaggcattc aggctgtggc ccaccggcag
     3841 aatatctttc agcagacgag tcgtcatttc ataggagatc atcgaggccc agcgggtttc
     3901 aatatatttc agtgccgggt gagaataatc gccggcccag tcgctgagca aactgactgt
     3961 ctttgtatca ctctcttcac aacggcaccg gtacacccga agcccggata ccggaataac
     4021 gccaaacagc gtccggtacc gtattttctg tttgcctttg attctgcgcg cagcaaggca
     4081 gtgagggcac cggatatggt gttgagtata ttcgtctgcc tgcagctgga ccaccgactg
     4141 ctggaccgta ttcagcaaca gcttggattc tgacaccgat aatccgatgt cgttccgggt
     4201 ctcccctgat ttctggattg tcatcagttc ctccgttcga ctggagcctg attcatcggt
     4261 gatgactatc tggagcgtca gttgcatcat tgcctcctgc tgtggtggat gactatctcc
     4321 cggctactgg ttgttcatct ggtgacgaag cttataatat gtggaaacgc caaggcccgc
     4381 atggtcgcag gcgatcctga gcctgacccc tctgcttctg agtgcgtcaa tttcctggat
     4441 ctttttgata tcggcgccgg ggcgccctgt tttttcaccc cgtgcttttt tcgcggcgat
     4501 gccgtcggcc tgcctttcct tcagtaaggc tcgttcaaac tggctgaatg ccgacatcat
     4561 gtgcagctgc agttcctgca tcggattgtt tgtgccggcg ctgaacgtca gctgctcttt
     4621 cagaaaaata agggtaacgc cctggtcacg aagccgggtg gtgacagcgc acatatccga
     4681 catattccgg caaaggcgat cgatggaatg aaccagaagc gtatcaccag ggcgtaattg
     4741 ccccaggagt tcctgtaacc ccgggcggtt tgtgtccttc gcgctggcat gctcaacaca
     4801 aattttatca ggctgaaaac ccgcctttga caatgcctct tcctgacggg ccgtattctg
     4861 atcggccgag cttaccctga tgtaggcata ttgcacgcaa aacctccgga aggtctttaa
     4921 acatcataga gatttaaaga ataatccata aacactgatt tagggaggtt tatagacatt
     4981 ttttcgggga tctgagaggc atgctgaaaa cgtatacctt tatagacatc agtcagcgac
     5041 gacggcccag ccagataact tcaggctcca gtaccggaga tgcaaggtcg agcctgctca
     5101 gttgccggtt gattttgtgg cggttgaacc tgacagcatc caggtcgtca gcaaaagggc
     5161 ggatctcgcc gaccgttgtt tcatcatccg cattaacgat agcttcaaga aacgccaggg
     5221 ttttatcgaa ctcatccgca gctgtggctc cgggcatacc atggccgctt atacaaaacg
     5281 gcgctttcag cgtagagttt tcataaatgg cctcaacacg aatgtcatga agccagtgct
     5341 caaagaaatt gtattcatag gtaaagcgat cgccagcatc aaaag
//
LOCUS       DBBBSB010000431         4295 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.226, whole genome shotgun sequence.
ACCESSION   DBBBSB010000431 DBBBSB010000000
VERSION     DBBBSB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.226"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            558..797
                     /locus_tag="ACIUS0_004725"
     CDS             558..797
                     /locus_tag="ACIUS0_004725"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HGB0222099.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACIUS0_004726"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACIUS0_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004727"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HGB0222100.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACIUS0_004728"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACIUS0_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HGB0222101.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4282
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004729"
                     /pseudo
     CDS             3444..4282
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1207 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gttcactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgac
     4261 gccgcttcgc ggcgcggctt aactcaagcg ttaga
//
LOCUS       DBBBSB010000432         4292 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.227, whole genome shotgun sequence.
ACCESSION   DBBBSB010000432 DBBBSB010000000
VERSION     DBBBSB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.227"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            558..797
                     /locus_tag="ACIUS0_004730"
     CDS             558..797
                     /locus_tag="ACIUS0_004730"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HGB0222102.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACIUS0_004731"
                     /pseudo
     CDS             complement(985..1962)
                     /gene="intI2"
                     /locus_tag="ACIUS0_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004732"
     CDS             2294..2767
                     /gene="dfrA1"
                     /locus_tag="ACIUS0_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HGB0222103.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2862..3386
                     /gene="sat2"
                     /locus_tag="ACIUS0_004733"
     CDS             2862..3386
                     /gene="sat2"
                     /locus_tag="ACIUS0_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HGB0222104.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3444..4279
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004734"
                     /pseudo
     CDS             3444..4279
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         1204 a          943 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagctg tgggcggaca
       61 aaatagttgg gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt
      121 gacaaaatag atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca
      181 aaatagtttg gaactagatt tcacttatct ggttaactaa tctagctaac ctgccgctca
      241 gcttagtacg atcctatatc caactgatca taatcctttc atcagtatcc caaattaaat
      301 ccccctccta gactgcctca ccaacacttt acagtgatcc cgtatttccg gacaggtgat
      361 tgattttttt cgcttgtaca ggtgttaagc cacagttatc ttgatgtgga cattatgcat
      421 tgtaatagcc cattaacatg cgctaagagc ataaattaat atacagtaac aagcattacg
      481 aggtgctaat gtttggaaag cagcctcttc caactacaat aataagttaa ctatcctcca
      541 cccacaaata attacaaatg acccctttcc gctataactc cgatctcacc agcggctcgc
      601 tacaaacccg agagtgtcgc attattactg gcttgttact ccaagagctt gatgaagctg
      661 cctgggataa agccatgtat aaggaaaatg tgcttcagaa acgcacgcaa tctacggtaa
      721 gacgtatttc ttcagcgctt agaaaacgcc tagaacacct aagctctgac ttttgggctt
      781 ttgcgttttt atgttaggca ttgtctggca actggaggaa gtgtaatgac catgaatggt
      841 cactgtgatt gacagtatct tgcttaaact tatctgaaca atggtagaac tggctcatat
      901 ttatcccctc tatgattgtt tatctttttc accataaaaa gtatcagggg ctactcaggg
      961 ctacataaag atatttatct acttttactg attgataagt agcatcagtc catccgcagg
     1021 actggtggtg ccggcaaaat gctgacccaa cacatgcgta tagatttgcg tggtcttaac
     1081 atcgttatgc cctaagagtt cttgcacagt gcgaatatca cgccccgctt gtaatagatg
     1141 cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc ttgctaacga tgcctgcttt
     1201 ttgtacggct gccttcaatg cctttcgcgc aacggagtca tgcagatgat ggcggcataa
     1261 tttgccgtta tacgggtggt tgcagagcgt gctggaggga aagacaaaca tccacgccgc
     1321 ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa ggcagcgatg gccctacgcc
     1381 ttgtaagttg tcgtcttgct gaataagccg cgcttgctca atgagttatt ttattgctgg
     1441 gattaggcgc gtgggcagta ggctgtttct gcttttccca cccttaccgt catgcacagt
     1501 gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa cgcaagcatt cattaatgcg
     1561 caaacctgca ccatacagca gcgtaaaaat aacttggttg cgagtatcca taacctgcaa
     1621 aatgcgttgc acttcatttg cagagataac agagggtagc cgtctaggct tgcttgcagg
     1681 gatataatca atatcgccca acggctgttg taaaaacctg ttgtacaaaa aagctagggc
     1741 atttaaagcg attttctgcg tgtttatggc tacatgtctg ctgtttgcta agctggataa
     1801 aaacagcctg acctcttcac tgcccatggt ctgaggatga cgttttttgt gaaacagaat
     1861 aaaacgctta atccagtgca ggtaagtttt ttcagttttc agcgcataac ctttttgtcg
     1921 catatccgtg cgtatagaat ttaaaaatgg actgttagac ataaaacgct ccttgtcttg
     1981 caactgtctg cctatacagc ctattctagc tgggatttaa aaaagtgcct gttttttacg
     2041 cctagagatg cttgtttacc ggtagagttt taatttaatg ctaaataaat taaaatgtta
     2101 tgagttcttt gggtgagata atgtgcatcg tgcaagcagg atagacggca tgcacgattt
     2161 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
     2221 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
     2281 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
     2341 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
     2401 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
     2461 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
     2521 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
     2581 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
     2641 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
     2701 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
     2761 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
     2821 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
     2881 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
     2941 atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga
     3001 aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg
     3061 accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg
     3121 aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat
     3181 ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga
     3241 acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc
     3301 tgttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt
     3361 tctcgggagc acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg
     3421 gcttaattca ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg
     4141 gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     4201 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     4261 gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DBBBSB010000433         2619 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.282, whole genome shotgun sequence.
ACCESSION   DBBBSB010000433 DBBBSB010000000
VERSION     DBBBSB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.282"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..369
                     /locus_tag="ACIUS0_004735"
     CDS             <1..369
                     /locus_tag="ACIUS0_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGB0222105.1"
                     /translation="VSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITL
                     SGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLG
                     YREVTITEKGYAAFKTHFHI"
     gene            complement(347..970)
                     /gene="tetR(B)"
                     /locus_tag="ACIUS0_004736"
     CDS             complement(347..970)
                     /gene="tetR(B)"
                     /locus_tag="ACIUS0_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HGB0222106.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1052..2257
                     /gene="tet(B)"
                     /locus_tag="ACIUS0_004737"
     CDS             1052..2257
                     /gene="tet(B)"
                     /locus_tag="ACIUS0_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HGB0222107.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2370..>2619)
                     /locus_tag="ACIUS0_004738"
     CDS             complement(2370..>2619)
                     /locus_tag="ACIUS0_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HGB0222108.1"
                     /translation="IIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLL
                     NGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          672 a          507 c          555 g          885 t
ORIGIN      
        1 gtttccacgc cagtgcattt acgaaaagca cgtacttgct atgatcattt agctggcgaa
       61 gttgccgtta agatctatga ttccctttgt caacagcaat ggatcactga aaatggttca
      121 atgatcacat taagtggtat tcaatatttt catgaaatgg gaattgacgt tccttccaaa
      181 cattcacgta aaatctgttg tgcgtgttta gattggagtg aacgccgttt ccatttaggt
      241 gggtacgttg gagccgcatt attttcgctt tatgaatcta aagggtggtt aactcgacat
      301 cttggttacc gtgaagttac catcacggaa aaaggttatg ctgcttttaa gacccacttt
      361 cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg
      421 gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat
      481 cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc
      541 aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac
      601 cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc
      661 gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt
      721 tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat
      781 ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat
      841 gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac
      901 cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta
      961 atctagacat cattaattcc taatttttgt tgacactcta tcattgatag agttatttta
     1021 ccactcccta tcagtgatag agaaaagtga aatgaatagt tcgacaaaga tcgcattggt
     1081 aattacgtta ctcgatgcca tggggattgg ccttatcatg ccagtcttgc caacgttatt
     1141 acgtgaattt attgcttcgg aagatatcgc taaccacttt ggcgtattgc ttgcacttta
     1201 tgcgttaatg caggttatct ttgctccttg gcttggaaaa atgtctgacc gatttggtcg
     1261 gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg gattacttat tgctggcttt
     1321 ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt tcagggatca caggagctac
     1381 tggggctgtc gcggcatcgg tcattgccga taccacctca gcttctcaac gcgtgaagtg
     1441 gttcggttgg ttaggggcaa gttttgggct tggtttaata gcggggccta ttattggtgg
     1501 ttttgcagga gagatttcac cgcatagtcc cttttttatc gctgcgttgc taaatattgt
     1561 cgctttcctt gtggttatgt tttggttccg tgaaaccaaa aatacacgtg ataatacaga
     1621 taccgaagta ggggttgaga cgcaatcgaa ttcggtatac atcactttat ttaaaacgat
     1681 gcccattttg ttgattattt atttttcagc gcaattgata ggccaaattc ccgcaacggt
     1741 gtgggtgcta tttaccgaaa atcgttttgg atggaatagc atgatggttg gcttttcatt
     1801 agcgggtctt ggtcttttac actcagtatt ccaagccttt gtggcaggaa gaatagccac
     1861 taaatggggc gaaaaaacgg cagtactgct cggatttatt gcagatagta gtgcatttgc
     1921 ctttttagcg tttatatctg aaggttggtt agttttccct gttttaattt tattggctgg
     1981 tggtgggatc gctttacctg cattacaggg agtgatgtct atccaaacaa agagtcatca
     2041 gcaaggtgct ttacagggat tattggtgag ccttaccaat gcaaccggtg ttattggccc
     2101 attactgttt gctgttattt ataatcattc actaccaatt tgggatggct ggatttggat
     2161 tattggttta gcgttttact gtattattat cctgctatcg atgaccttca tgttaacccc
     2221 tcaagctcag gggagtaaac aggagacaag tgcttagtta tttcgtcacc aaatgatgtt
     2281 attccgcgaa atataatgac cctcttgata acccaagagg gcatttttta cgataaagaa
     2341 gatttagctt caaataaaac ctatctattt tatttatctt tcaagctcaa taaaaagccg
     2401 cggtaaatag caataaattg gcctttttta tcggcaagct cttttaggtt tttcgcatgt
     2461 attgcgatat gcataaacca gccattgagt aagtttttaa gcacatcatc atcataagct
     2521 ttaagttggt tctcttggat caatttgctg acaatggcgt ttaccttacc agtaatgtat
     2581 tcaaggctaa ttttttcaag ttcattccaa ccaatgata
//
LOCUS       DBBBSB010000434         2184 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.298, whole genome shotgun sequence.
ACCESSION   DBBBSB010000434 DBBBSB010000000
VERSION     DBBBSB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.298"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            175..1050
                     /locus_tag="ACIUS0_004739"
     CDS             175..1050
                     /locus_tag="ACIUS0_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-15"
                     /protein_id="HGB0222109.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQS
                     GGRLGVALINTADNSQILYRADERFAMCSTSKVMAAAAVLKKSESEPNLLNQRVEIKK
                     SDLVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGD
                     ETFRLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNT
                     TGAASIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESR
                     RDVLASAAKIVTDGL"
     gene            complement(1097..1441)
                     /locus_tag="ACIUS0_004740"
                     /pseudo
     CDS             complement(1097..1441)
                     /locus_tag="ACIUS0_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
     gene            1443..2184
                     /locus_tag="ACIUS0_004741"
                     /pseudo
     CDS             1443..2184
                     /locus_tag="ACIUS0_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
BASE COUNT          531 a          561 c          596 g          496 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccaa taatgttaca atgtgtgaga
       61 agcagtctaa attcttcgtg aaatagtgat ttttgaagct aataaaaaac acacgtggaa
      121 tttagggact attcatgttg ttgttatttc gtatcttcca gaataaggaa tcccatggtt
      181 aaaaaatcac tgcgccagtt cacgctgatg gcgacggcaa ccgtcacgct gttgttagga
      241 agtgtgccgc tgtatgcgca aacggcggac gtacagcaaa aacttgccga attagagcgg
      301 cagtcgggag gcagactggg tgtggcattg attaacacag cagataattc gcaaatactt
      361 tatcgtgctg atgagcgctt tgcgatgtgc agcaccagta aagtgatggc cgcggccgcg
      421 gtgctgaaga aaagtgaaag cgaaccgaat ctgttaaatc agcgagttga gatcaaaaaa
      481 tctgaccttg ttaactataa tccgattgcg gaaaagcacg tcaatgggac gatgtcactg
      541 gctgagctta gcgcggccgc gctacagtac agcgataacg tggcgatgaa taagctgatt
      601 gctcacgttg gcggcccggc tagcgtcacc gcgttcgccc gacagctggg agacgaaacg
      661 ttccgtctcg accgtaccga gccgacgtta aacaccgcca ttccgggcga tccgcgtgat
      721 accacttcac ctcgggcaat ggcgcaaact ctgcggaatc tgacgctggg taaagcattg
      781 ggcgacagcc aacgggcgca gctggtgaca tggatgaaag gcaataccac cggtgcagcg
      841 agcattcagg ctggactgcc tgcttcctgg gttgtggggg ataaaaccgg cagcggtggc
      901 tatggcacca ccaacgatat cgcggtgatc tggccaaaag atcgtgcgcc gctgattctg
      961 gtcacttact tcacccagcc tcaacctaag gcagaaagcc gtcgcgatgt attagcgtcg
     1021 gcggctaaaa tcgtcaccga cggtttgtaa tagcggaaac ggaatgggga aactcattcc
     1081 gtttttgttt atcgccttag acggcaaaag tgctgtcgcc cacctgcgct tgcgcatacc
     1141 aggccataag ctccgtggtt cctggttctc cttccgctgg agcccagtgc gcatagtcat
     1201 cggcagccac gggttgatag ccaccgtgtt ttacttcaaa aattatgcca ccggtatcca
     1261 gcgacagcac ggcatgccag gttcctgcgg ccatctccag caccgtacag gtttccccca
     1321 atatcgcccg atgggtgacg gtaccccgat cgtcaaaatt cagcaccacg aaacgacccc
     1381 ttaatggcaa cagtagctcg aaggtgtgag ggtgtcggtg cgggcgcacg taggtcccag
     1441 gtcatattcc ttccggcgtc cggcatttta ccgccagaca gctcgggatt cgtgatatca
     1501 ccgttcttgc agaatacggt cagagggaaa atacccgccg tgagcatgca gcgctgatac
     1561 gtcagcacta tcagtatcgt gaatttgcct ggccctggac atttcgcctt acccgtcttt
     1621 tatatacccg gagctggata agcaacgaac gtcctggcct gcttttcgat ctggcgacag
     1681 ggtggcttat gcaacatcgt attattctcc ccggagccac tacgctgacc cggttgattt
     1741 cagaggtaag ggaaaaggcg acgttgcgcc tgtggaacaa actggcactg ataccgtcag
     1801 ccgaacagcg ttcacagctg gagatgctgc tggggccaac tgattgcagc cgcctgtctt
     1861 tactggaatc actgaaaaag ggccctgtga ccatcagtgg tccggcgttt aatgaagcaa
     1921 ttgaacgctg gaaaactctg aacgattttg gcctgcatgc tgaaaacctg agtacactcc
     1981 cggctgtgcg cctgaaaaat ctcgcacgtt atgctggtat gacttcggtg ttcaatattg
     2041 ccaggatgtc accgcagaaa aggatggcgg ttctggttgc ctttgtcctt gcatgggaaa
     2101 cgctggcgct ggatgatgca ttggacgttc tggacgccat gctggccgtt atcatccgtg
     2161 acgccagaaa gattgggcag aaaa
//
LOCUS       DBBBSB010000435          936 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.367, whole genome shotgun sequence.
ACCESSION   DBBBSB010000435 DBBBSB010000000
VERSION     DBBBSB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.367"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            85..923
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004742"
                     /pseudo
     CDS             85..923
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          229 a          241 c          253 g          213 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccctc ccctcgctgg cgctcggcgc
       61 cccttatttc aaacgttaaa catcatgagg gaagtggtga tcgccgaagt atcgactcaa
      121 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      181 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
      241 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
      301 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
      361 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
      421 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
      481 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
      541 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
      601 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
      661 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
      721 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
      781 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
      841 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
      901 cgccgcttcg cggcgcggct taactcaagc gttaga
//
LOCUS       DBBBSB010000436          933 bp    DNA     linear   BCT 06-NOV-2024
DEFINITION  TPA_asm: Shigella flexneri 2a strain AUSMDU00099713
            SAMN44443322-rid23908073.guided.369, whole genome shotgun sequence.
ACCESSION   DBBBSB010000436 DBBBSB010000000
VERSION     DBBBSB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN44443322
            Sequence Read Archive: SRR31237581
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-NOV-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002457399.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002457399.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/06/2024 10:24:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,743
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,168
            CDSs (with protein)               :: 4,168
            Genes (RNA)                       :: 90
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 79
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 485
            CDSs (without protein)            :: 485
            Pseudo Genes (ambiguous residues) :: 0 of 485
            Pseudo Genes (frameshifted)       :: 218 of 485
            Pseudo Genes (incomplete)         :: 171 of 485
            Pseudo Genes (internal stop)      :: 171 of 485
            Pseudo Genes (multiple problems)  :: 68 of 485
            Pseudo Genes (short protein)      :: 1 of 485
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN44443322-rid23908073.guided.369"
                     /strain="AUSMDU00099713"
                     /serotype="2a"
                     /host="Homo sapiens"
                     /db_xref="taxon:42897"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2024-04-24"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            85..920
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004743"
                     /pseudo
     CDS             85..920
                     /gene="aadA1"
                     /locus_tag="ACIUS0_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          226 a          241 c          253 g          213 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccctc ccctcgctgg cgctcggcgc
       61 cccttatttc aaacgttaaa catcatgagg gaagtggtga tcgccgaagt atcgactcaa
      121 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
      181 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
      241 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
      301 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
      361 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
      421 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
      481 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
      541 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
      601 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
      661 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
      721 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctaggca ggcttatctt
      781 ggacaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
      841 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
      901 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
